scholarly journals Effects of genetic variation in the 20th intron of Sansui duck ATP2A2 gene on eggshell quality

2021 ◽  
Vol 3 (2) ◽  
Author(s):  
Junhan Mei ◽  
Yiyu Zhang ◽  
Minfang You ◽  
Chaomei Liao ◽  
Guanghui Tan ◽  
...  
Keyword(s):  
Direct Sequencing ◽  
Strong Linkage Disequilibrium ◽  
Strong Linkage ◽  
Weinberg Equilibrium ◽  
Atp2a2 Gene ◽  
Eggshell Quality ◽  
Hardy Weinberg Equilibrium ◽  
Intron Region ◽  
The Relationship

In order to explore the influence of the polymorphism in the 20 intron region of the Sansui duck ATP2A2 gene on the eggshell quality, this study used Primer Premier 5 software to design and synthesize a pair of primers in the 20 intron region, two-way direct sequencing and sequence alignment to mine SNPs Sites, SPSS 18.0 software was used to analyze the relationship between SNP sites and eggshell quality of Sansui duck. Results Three SNP sites were found in the 20 intron region of the ATP2A2 gene: g.40874 T>C, g.40920 G>A and g.40990 T=C, all of which were moderately polymorphic, at the site g.40874 T ＞C and g.40920 G＞A both deviated significantly from Hardy-Weinberg equilibrium (P>0.05), position g.40990 T=C accords with Hardy-Weinberg equilibrium (P<0.05), and position g.40874 T＞C There is a strong linkage disequilibrium between g.40990 T=C; a total of 4 haplotypes and 9 double types were detected at 3 SNP loci; the results of association analysis show that g.40874 T＞C mutation has an effect on eggshell strength The eggshell strength of CC genotype was significantly higher than that of TC and TT genotypes (P<0.05), and the eggshell weight of CC genotype was significantly higher than that of TC genotype (P<0.05), g. The 40990 T=C mutation has a significant effect on the eggshell strength, and the eggshell strength of the TC genotype is significantly higher than that of the TT genotype (P<0.05). In summary, the g.40874 T>C and g.40990 T=C found in the 20th intron region of the Sansui duck ATP2A2 gene may be the marker sites that affect the quality of the eggshell.

scholarly journals Novel Mutation of Exon 5 Prolactin Gene in IPB-D1 Chicken

2020 ◽  
Vol 25 (4) ◽  
pp. 173
Author(s):  
Lailatul Rohmah ◽  
Sri Darwati ◽  
Niken Ulupi ◽  
Isyana Khaerunnisa ◽  
Cece Sumantri
Keyword(s):  
Amino Acids ◽  
Dna Sequences ◽  
Egg Production ◽  
Novel Mutation ◽  
Direct Sequencing ◽  
Dna Amplification ◽  
Weinberg Equilibrium ◽  
Synonymous Mutations ◽  
Prolactin Gene ◽  
Hardy Weinberg Equilibrium

<p class="JITV-ReferenceIsi">The prolactin gene (PRL) is a gene that controls the incubation and egg production in laying chickens. The nature of incubation will reduce egg production and disrupt the reproductive system in local chickens. The purpose of this study was to identify the polymorphism of prolactin genes in IPB-D1 chickens using the direct sequencing method. The polymorphism of the exon 5 prolactin gene was carried out on 46 samples of IPB-D1 chicken DNA which was a collection of the Division of Animal Genetics and Breeding, Faculty of Animal Science IPB. DNA sequences as a reference for designing exon 5 primers were obtained from the National Center for Biotechnology Information (NCBI) with the GenBank access code: AF288765.2. DNA extraction was carried out using the phenol-chloroform technique. DNA amplification resulted in a PCR product with a size of 557 bp. In this study, the genotype frequency, allele frequency, heterozygosity value and Hardy-Weinberg equilibrium were calculated. The results of the study found 5 SNPs in exon 5, namely g.7823A&gt;G, g.7835A&gt;G, g.7886T&gt;A, g.8052T&gt;C, and g.8069T&gt;C. All SNPs are polymorphic and in Hardy-Weinberg equilibrium except g.8052T&gt;C. The g.7823A&gt;G, g.7835A&gt;G, g.8052T&gt;C SNPs are synonymous mutations that do not change amino acids, while the g.7886T&gt;A and g.8069T&gt;C SNPs are non-synonymous that change amino acids. Both g.7886T&gt;A and g.8069T&gt;C SNPs are potential as a marker assisted selection for the characteristics of egg production in IPB-D1 chickens.</p>

scholarly journals Polymorphism of Insulin-induced gene 1 (INSIG1) of three local beef cattle in Indonesia

2021 ◽  
Vol 888 (1) ◽  
pp. 012026
Author(s):  
S D Volkandari ◽  
E T Margawati ◽  
A S Wulandari ◽  
Indriawati
Keyword(s):  
Beef Cattle ◽  
Regulatory Element ◽  
Growth Trait ◽  
Weinberg Equilibrium ◽  
Pcr Rflp ◽  
Hardy Weinberg Equilibrium ◽  
Sterol Regulatory Element ◽  
Dna Fragment ◽  
Genotype And Allele Frequencies

Abstract Insulin-induced gene 1 (INSIG1) encoded protein that blocked of proteolysis activity from protein sterol regulatory element binding proteins (SREBP). The role gene plays of cholesterol, fatty acid, and glucose metabolism. Indonesia has many several beef cattle which has good quality of growth trait. The aim of this study was to identify polymorphism of INSIG1 gene (SNP 4366 (A>G) and 4534 (T>C)) of three local beef cattle in Indonesia (Bali, Pasundan and Ongole Decendent). One hundred and twenty seven samples were used in this study consisting of Bali cattle (46) from Pulukan Bali Island, Pasundan (36) from UPTD BPPT Beef cattle Ciamis West Java and Ongole Grade (OD) (45). DNA was extracted from whole blood using High Salt method then genotyping used PCR-RFLP method with RsaI and TaqI enzymes. Parameters in this study were genotype and allele frequencies, heterozigosity observed (Ho), expected (He), Hardy-Weinberg Equilibrium (HWE) and Polymorphism Information Content (PIC). Result showed that a 428 bp of DNA fragment was successfully amplified and digested. Three variant of genotypes with two alleles were identified. For SNP 4366 (A>G) were AA, AG and GG with dominant of G allele while SNP 4534 (T>C) were CC, CT and TT with dominant C allele. Both of SNPs in each of breed populations was in Hardy-Weinberg Equilibrium and polymorphic. Additionally, PIC value reached moderate. In conclusion, polymorphism was found in beef cattle and it could be early study for genetic diversity

scholarly journals Body size characteristics and polymorphism in GH and GHRH genes of Simeulue Buffalo of Aceh, Indonesia

2018 ◽  
Vol 20 (1) ◽  
pp. 236-242 ◽  
Author(s):  
KARTINI ERIANI ◽  
RAHMI RAHMI ◽  
IKHWAN JAMIL ◽  
ROSNIZAR ROSNIZAR ◽  
AL AZHAR
Keyword(s):  
Body Size ◽  
Coefficient Of Variation ◽  
Molecular Data ◽  
Mean Value ◽  
Weinberg Equilibrium ◽  
Genotype Frequencies ◽  
Size Characteristic ◽  
Hardy Weinberg Equilibrium ◽  
The Relationship ◽  
Age Category

Eriani K, Rahmi R, Jamil I, Rosnizar R, Azhar A. 2019. Body size characteristics and polymorphism in GH and GHRH genes of Simeulue Buffalo of Aceh, Indonesia. Biodiversitas 20: 236-242. This study was done to determine body size characteristics, to identify the presence of polymorphism in the GH/MspI and GHRH/HaeIII genes, and to analyze the relationship between GH and GHRH gene polymorphisms to body size characteristics in Simeulue buffalo. Experimental animals used were 45 Simeulue buffalo randomly sampled from three subdistricts, i.e., Salang, Alafan and Simeulue Cut, fifteen from each subdistrict. Body size characteristics measured were withered height, body length, chest circumference, chest depth, and hip height. A 5 mL whole blood was collected from fifteen out of forty five by using EDTA as an anticoagulant. Extraction of genomic DNA and analysis of GH/MspI and GHRH/HaeIII polymorphisms was done using GeneAid DNA extraction kit and polymerase chain reaction-restriction length fragment polymorphism (PCR-RFLP) method, respectively. The data of body size characteristics which includes mean value, standard deviation, and coefficient of variation were analyzed descriptively. The molecular data represented by DNA banding pattern was used to calculate allele and genotype frequencies, expected (He) and observed (Ho) heterozygosities, and Hardy-Weinberg equilibrium. The relationship between genotype and body size characteristic was analyzed using t-test. The results showed that coefficient of variation in body size characteristics was low, ranging from 5.39-16.06% in the age category of 4-6 years old and ranging from 5.25-11.27% in the age category of 2- 4 years old. The results of the molecular analysis indicated that GH/MspI locus was monomorphic by the presence of one (+/+) genotype. GHRH/HaeIII locus was polymorphic as shown by the presence of 3 genotypes namely AA (0.13), AB (0.80) and BB (0.07). Expected heterozygosity (0.80) was higher than observed heterozygosity (0.49). The frequency of GHRH/HaeIII agreed with Hardy Weinberg equilibrium. GHRH/HaeIII polymorphism did not significantly influence (P>0.05) body size characteristics of Simeulue buffalo.

The Relationship Between Interoception and Metacognition

2016 ◽  
Vol 30 (2) ◽  
pp. 76-86 ◽  
Author(s):  
Judith Meessen ◽  
Verena Mainz ◽  
Siegfried Gauggel ◽  
Eftychia Volz-Sidiropoulou ◽  
Stefan Sütterlin ◽  
...  
Keyword(s):  
Memory Performance ◽  
Memory Task ◽  
Demographic Characteristics ◽  
Metacognitive Awareness ◽  
Interoceptive Awareness ◽  
Future Research ◽  
Heartbeat Perception ◽  
Interoceptive Accuracy ◽  
The Relationship

Abstract. Recently, Garfinkel and Critchley (2013) proposed to distinguish between three facets of interoception: interoceptive sensibility, interoceptive accuracy, and interoceptive awareness. This pilot study investigated how these facets interrelate to each other and whether interoceptive awareness is related to the metacognitive awareness of memory performance. A sample of 24 healthy students completed a heartbeat perception task (HPT) and a memory task. Judgments of confidence were requested for each task. Participants filled in questionnaires assessing interoceptive sensibility, depression, anxiety, and socio-demographic characteristics. The three facets of interoception were found to be uncorrelated and interoceptive awareness was not related to metacognitive awareness of memory performance. Whereas memory performance was significantly related to metamemory awareness, interoceptive accuracy (HPT) and interoceptive awareness were not correlated. Results suggest that future research on interoception should assess all facets of interoception in order to capture the multifaceted quality of the construct.

The relationship between optimism, personal control and quality of life of patients with COPD

2002 ◽  
Author(s):  
R. Arnold ◽  
A. V. Ranchor ◽  
N. H. T. ten Hacken ◽  
G. H. Koeter ◽  
V. Otten ◽  
...  
Keyword(s):  
Quality Of Life ◽  
Personal Control ◽  
The Relationship

Using the alveograph in developing requirements for the quality of flour for wafer sheet production

2020 ◽  
Vol 29 (12) ◽  
pp. 52-58
Author(s):  
E.P. Meleshkina ◽  
◽  
S.N. Kolomiets ◽  
A.S. Cheskidova ◽  
◽  
...  
Keyword(s):  
Rheological Properties ◽  
Quality Indicators ◽  
Wheat Flour ◽  
Mathematical Statistics ◽  
Intended Purpose ◽  
The Future ◽  
Relationship Of ◽  
The Relationship

Objectively and reliably determined indicators of rheological properties of the dough were identified using the alveograph device to create a system of classifications of wheat and flour from it for the intended purpose in the future. The analysis of the relationship of standardized quality indicators, as well as newly developed indicators for identifying them, differentiating the quality of wheat flour for the intended purpose, i.e. for finished products. To do this, we use mathematical statistics methods.

HUBUNGAN KUALITAS KEHIDUPAN KERJA TERHADAP KINERJA PERAWAT PELAKSANA

2019 ◽  
Vol 9 (4) ◽  
pp. 413-420
Author(s):  
Amella Gusty ◽  
Dachriyanus Dachriyanus ◽  
Leni Merdawati
Keyword(s):  
District Hospital ◽  
Simple Random Sampling ◽  
Work Life ◽  
Quality Of Work Life ◽  
P Value ◽  
Work Context ◽  
Cross Sectional ◽  
Quality Of Work ◽  
The Relationship

Kinerja perawat merupakan hasil yang dicapai dalam melaksanakan asuhan keperawatan di rumah sakit. Terciptanya asuhan keperawatan yang optimal sangat diperlukan dukungan dari pihak rumah sakit salah satunya adalah menciptakan kualitas kehidupan kerja baik bagi perawat. Penelitian ini bertujuan untuk mengetahui hubungan antara kualitas kehidupan kerja terhadap kinerja perawat pelaksana di RSUD Teluk Kuantan. Jenis penelitian kuantitatif dengan pendekatan cross sectional. Jumlah sampel 120 responden yang diperoleh melalui proportionate simple random sampling. Hasil penelitian menunjukkan kualitas kehidupan kerja perawat RSUD Teluk Kuantan berada pada kategori sedang, kinerja perawat berada pada kategori kurang baik. Berdasarkan analisis kedua variabel teridentifikasi bahwa tidak ada hubungan antara kualitas kehidupan kerja dengan kinerja perawat pelaksana dengan  p value 0,817. Dimensi work context merupakan komponen yang memiliki hubungan dengan kinerja perawat pelaksana dengan p value 0,008, dimensi work life  tidak berhubungan secara signifikan dengan kinerja perawat. Kesimpulan penelitian diketahui tidak ada hubungan antara kualitas kehidupan kerja dengan kinerja perawat pelaksana. Ada hubungan dimensi work context dengan kinerja perawat pelaksana. Tidak ada hubungan dimensi work life dengan kinerja perawat.   Kata kunci: kualitas kehidupan kerja, kinerja, perawat pelaksana   THE RELATIONSHIP BETWEEN THE QUALITY OF WORK LIFE AND THE PERFORMANCE OF IMPLEMENTERS NURSES   ABSTRACT Nurse performance is the result achieved in implementing nursing care in a hospital. The creation of optimal nursing care is very much needed support from the hospital, one of which is to create a good quality of work life for nurses. This study aims to determine the relationship between the quality of work life and the performance of nurses at the Kuantan District Hospital. This type of quantitative research with cross sectional approach. The number of samples of 120 respondents obtained through proportionate simple random sampling. The results showed that the quality of work life of nurses at the Kuantan District Hospital was in the medium category, the nurses' performance was in the unfavorable category. Based on the analysis of the two variables, it was identified that there was no relationship between the quality of work life with the performance of implementing nurses with a p value of 0.817. The work context dimension is a component that has a relationship with the performance of nurses implementing with p value 0.008, the dimension of work life does not significantly correlate with nurse performance. The conclusion of the research is that there is no relationship between the quality of work life and the performance of the implementing nurses. There is a relationship between the dimensions of the work context and the performance of the nurses. There is no relationship between the dimensions of work life and nurse performance.   Keywords: quality of work life, performance, implementers nurse

Management of Economics and State "Bottom"

2020 ◽  
Vol 17 (6) ◽  
pp. 76-91
Author(s):  
E. D. Solozhentsev
Keyword(s):  
Quality Of Life ◽  
Artificial Intelligence ◽  
Probabilistic Models ◽  
Human Life ◽  
Special Software ◽  
Human Quality ◽  
Relationship Of ◽  
The Relationship

The scientific problem of economics “Managing the quality of human life” is formulated on the basis of artificial intelligence, algebra of logic and logical-probabilistic calculus. Managing the quality of human life is represented by managing the processes of his treatment, training and decision making. Events in these processes and the corresponding logical variables relate to the behavior of a person, other persons and infrastructure. The processes of the quality of human life are modeled, analyzed and managed with the participation of the person himself. Scenarios and structural, logical and probabilistic models of managing the quality of human life are given. Special software for quality management is described. The relationship of human quality of life and the digital economy is examined. We consider the role of public opinion in the management of the “bottom” based on the synthesis of many studies on the management of the economics and the state. The bottom management is also feedback from the top management.

THE IMPLEMENTATION OF ISO 9001:2015 TO IMPROVE QUALITY OF SERVICE AT PUSAT KESIHATAN UNIVERSITI (PKU), UNIVERSITI UTARA MALAYSIA(UUM)

2018 ◽  
Vol 13 (Number 2) ◽  
pp. 67-77
Author(s):  
Anis Syamimi Abd Rahim ◽  
Mohd Norhasni Mohd Asaad
Keyword(s):  
Quality Of Service ◽  
Customer Satisfaction ◽  
Descriptive Statistics ◽  
Iso 9001 ◽  
Quality Of Services ◽  
Research Questions ◽  
Spss Software ◽  
The Relationship ◽  
Universiti Utara Malaysia

The purpose of this study is to review the implementation of ISO 9001:2015 in order to improve the quality of services at Pusat Kesihatan Universiti (PKU), Universiti Utara Malaysia. The respondents of this study were customers at the PKU, UUM. The questionnaire was distributed to 50 respondents. The data were analyzed using SPSS software version 24. The data were tested using descriptive statistics, and correlation analysis to answer the research questions and to achieve the objectives. The findings show that customers agree that implementation of ISO 9001:2015 give service at PKU, UUM is good and satisfied. Through the correlation test, the results showed that the relationship between the implementation of ISO 9001:2015 has a positive and significant impact on customer satisfaction and the effect of implementing ISO 9001:2015 has a positive and significant impact in improving quality of service at PKU, UUM.Through mean and standard deviation tests, results show that tangible dimensions are the main dimensions of customer satisfaction while dimensions with low values are dimensions of responsiveness.Therefore, all aspects of service in PKU, UUM will be strengthened and all aspects of the weaknesses could be addressed to improve the service in order to maintain good quality services.

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