Uji Aktivitas Antioksidan Ekstrak Metanol Biji Kesumba Keling (Bixa orellana L)

Kesumba keling (Bixa orellana L) has been widely used as a natural dye on lips, hair, and cloth. The red pigment in kesumba keling seeds comes from a bixin and norbixin compound which have many conjugated double bonds, so it has the potential of antioxidants. This study aims to determine the antioxidant compound and an antioxidant activity of methanol extract of kesumba keling seeds. The moisture content of kesumba keling seeds is 78.74%. The powder of kesumba keling seeds was extracted using maceration method with 80% of methanol. Phytochemical test results of methanol extract positively contained flavonoid compound. An antioxidant activity test of methanol extract of kesumba keling seeds was carried out by determining DPPH free radical deterrent activity. The absorbance measurement were made using a UV-Vis spectrophotometer at a wavelength of 517 nm. Kesumba keling seeds extract has antioxidant activity which is indicated by IC50 value of 69.425 ppm, so it is classified as a strong antioxidant.

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ISOLATION OF ANTIOXIDANT COMPOUNDS OF METHANOL EXTRACT OF NUTMEG LEAVES (Myristica fragrans Houtt)

Jurnal Natural ◽

10.24815/jn.v17i1.6998 ◽

2017 ◽

Vol 17 (1) ◽

pp. 49

Author(s):

Nurmilasari Nurmilasari ◽

Binawati Ginting ◽

Hira Helwati

Keyword(s):

Antioxidant Activity ◽

Methanol Extract ◽

Chloroform Extract ◽

Antioxidant Compounds ◽

Test Results ◽

Antioxidant Assay ◽

Myristica Fragrans ◽

Ic50 Value ◽

High Antioxidant Activity ◽

Myristica Fragrans Houtt

Abstract.The antioxidant activity from methanol extract of nutmeg leaves (Myristica fragrans Houtt) was performed using 2,2-diphenyl-1-picrylhydrazil (DPPH). Antioxidant assay using DPPH of the methanol extract at a concentration of 25 ppm, 50 ppm and 100 ppm, showed high antioxidant activity with IC50 = 36.31 ppm and Vitamin C showed antioxidant activity with IC50 = 3.657 ppm. The methanol extract of nutmeg leaves were partitioned with chloroform to obtain chloroform extract of the leaves with IC50 = 28.30 ppm. isolation of the active compounds from the chloroform extract of the nutmeg leaf using column chromatography yielded 6 mix fractions MFMD 1, MFMD 2, MFMD 3, MFMD 4, MFMD 5 and MFMD 6. The test results of antioxidant activity in very strong category is fractions MFMD 4, MFMD 3, MFMD 2, MFMD 6 and MFMD 5 with IC50 of each are 26.590 ppm, 27.239 ppm, 29.639 ppm, 39.766 ppm and 55.436 ppm, while antioxidant activity in a weak catagory is fraction MFMD 1 with IC50 is 126.270 ppm. This shows that the fraction of the leaf has a better inhibitory activity, because some of the active compound contained in the fraction can synergize in inhibiting free radicals, so that the IC50 value of the fraction is stronger than the extract. Keywords: Antioxidant, chromathography, Nutmeg (Myristica fragransHoutt), methanol extract, leaves

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FITOKIMIA DAN AKTIVITAS ANTIOKSIDAN EKSTRAK KULIT BATANG Chisocheton sp. (C.DC) Harms (Meliaceae)

CHEMISTRY PROGRESS ◽

10.35799/cp.13.2.2020.31672 ◽

2020 ◽

Vol 13 (2) ◽

Author(s):

Dewa Gede Katja

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Methanol Extract ◽

Ethyl Acetate Extract ◽

Stem Bark ◽

Bark Extract ◽

Test Results ◽

Ic50 Value ◽

Dpph Method ◽

Stem Bark Extract

Tujuan penelitian ini untuk mengetahui golongan senyawa metabolit sekunder dan aktivitas antioksidan dari setiap ekstrak kulit batang Chisocheton sp. C.DC Harms (Meliaceae). Hasil ekstraksi 200 g serbuk kulit batang Chisocheton sp. C.DC Harms (Meliaceae) dengan n-heksana, etil asetat dan metanol masing-masing dengan 2000 mL berturut-turut menghasilkan 7,193 g ekstrak pekat n-heksana, 8,798 g ekstrak pekat etil asetat dan 18,683 g ekstrak pekat metanol. Hasil uji fitokimia menunjukkan adanya golongan senyawa flavonoid, triterpenoid dan tanin. Hasil uji aktivitas antioksidan dengan metode DPPH menunjukkan bahwa ekstrak n-heksana memberikan nilai IC50 sebesar 337,28 µg/mL, ekstrak metanol sebesar 216,73 µg/mL, dan ekstrak etil asetat sebesar 199,89 µg/mL yang berarti etil asetat memiliki kemampuan yang paling besar dalam menangkap radikal bebas.ABSTRACTThe objective of this study was determine the class of secondary metabolite compounds and antioxidant activity of each stem bark extract of Chisocheton sp. C.DC Harms (Meliaceae). The results of the extraction of 200 g Chisocheton sp. C.DC Harms (Meliaceae) with n-hexane, ethyl acetate and methanol with 2000 mL each yielded 7,193 g of concentrated extract n-hexane, 8,798 g of concentrated extract of ethyl acetate and 18,683 g of concentrated methanol extract. Phytochemical test results showed the presence of flavonoid, triterpenoid and tannin compounds. The results of the antioxidant activity test using the DPPH method showed that the n-hexane extract gave an IC50 value of 337,28 µg/mL, methanol extract was 216,73 µg/mL, and ethyl acetate extract was 199,89 µg/mL which means that ethyl acetate has the ability to greatest in capturing free radicals.

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Uji Aktivitas Antioksidan Ekstrak Daun Karet Kebo (Ficus Elastica) dengan Metode Peredaman Radikal Bebas Dpph (1,1-Diphenyl-2-Picrylhydrazil)

Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) ◽

10.22487/j24428744.2020.v6.i1.15022 ◽

2020 ◽

Vol 6 (1) ◽

pp. 141-150

Author(s):

Selpida Handayani ◽

Ida Kurniawati ◽

Faradiba Abdul Rasyid

Keyword(s):

Antioxidant Activity ◽

Methanol Extract ◽

Radical Scavenging ◽

Water Extract ◽

Free Radical Scavenging ◽

Water Extracts ◽

Activity Test ◽

Yield Value ◽

Ic50 Value ◽

Tlc Plates

Ficus elastica leaf is one of the Ficus species assumed to be rich in polyphenols so that it can act as an antioxidant. The research aimed to determine the antioxidant activity and IC50 value of methanol and water extracts of rubber leaf. In this research, the extraction was conducted by maceration using methanol and obtained the yield value of 7,03%, while the infundation using water obtained the yield value of 2,82%. The antioxidant activity test was conducted by DPPH free radical scavenging (1,1-diphenyl-2-phycrilhydrazyl) using a quercetin comparator. The qualitative tests were conducted by TLC method using dicloromethane:methanol eluent with a ratio of 99:1 for methanol extracts, and a ratio of 95:5 for water extracts, where all extracts of both methanol and water positively container antioxidants characterized by the formation of yellow spots with a purple background on TLC plates. The results of the quantitative test shower that the methanol extract had medium antioxidant activity with an IC50 value of 78,39 µg/mL, and water extract had an inactive antioxidant activity with an IC50 value of 319,11 µg/mL. While quercetin was classified as a very strong antioxidant with an IC50 value of 7,62 µg/mL.

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UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAUN BELUNTAS (Pluchea indica L.) DENGAN METODE DPPH (2, 2-DIFENIL-1-PIKRILHIDRAZIL)

Indonesian Chemistry and Application Journal ◽

10.26740/icaj.v2n2.p25-28 ◽

2019 ◽

Vol 2 (2) ◽

pp. 25

Author(s):

Defitiana Wanita

Keyword(s):

Antioxidant Activity ◽

Free Radical ◽

Chemical Compound ◽

Radical Reaction ◽

Ethanol Extract ◽

Free Radical Reaction ◽

Test Results ◽

Activity Test ◽

Ic50 Value ◽

Ethanol Ethanol

Antioxidant is chemical compound that can give one or more electron to free radical which can obstruct free radical reaction. Beluntas (Pluchea indica L.) is one of plant which potentially as antioxidant. The aim of this research is to determine the antioxidant activity of etanol extract of beluntas leaves. Antioxidant activity test was done by using DPPH (2, 2-diphenyl-1- picrylhydrazil) method. The dry powder of beluntas’s leaves was macerated by using ethanol. Ethanol extract obtained was tested antioxidant activity to get IC-50 value by using UV-Vis spectrophotometry at 517 nm. Antioxidant activity test results showed that ethanol extract of beluntas’s leaves had IC50 value of 37,25 ppm. So it had antioxidant activity in strong category.

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Phytochemical and Antioxidant Test of Binahong (Anredera cordifolia (Tenore) Steenis) Leaves Ethanol Extract

Fullerene Journal of Chemistry ◽

10.37033/fjc.v6i1.248 ◽

2021 ◽

Vol 6 (1) ◽

pp. 28

Author(s):

Fensia Analda Souhoka ◽

Imanuel Berly Delvis Kapelle ◽

Elisabeth Sihasale

Keyword(s):

Antioxidant Activity ◽

Phenolic Compounds ◽

Ethanol Extract ◽

Chemical Compounds ◽

Absorbance Measurement ◽

Activity Test ◽

Ic50 Value ◽

Dpph Method

Binahong Anredera cordifolia (Tenore) Steenis leaves contain chemical compounds that can be used as antioxidants. This study aims to examine the phytochemical and antioxidant activity of the ethanol extract of binahong leaves. Binahong leaves were extracted using the maceration method with an ethanol solvent. The phytochemical test showed that the ethanol extract of binahong leaves contained phenolic compounds, flavonoids, alkaloids, and tannins to have potential antioxidants. The antioxidant activity test was carried out using the DPPH method and absorbance measurement with a UV-Vis spectrophotometer at a wavelength of 517 nm. The results showed that the ethanol extract of binahong leaves had strong antioxidant activity, as evidenced by the IC50 value of 87.423 µg/mL.

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Antioxidant Activity, Inhibition α-Glucosidase of Ethanol Extract of Strychnos nitida G. Don and Identification of Active Compounds

Current Biochemistry ◽

10.29244/cb.5.3.11-20 ◽

2019 ◽

Vol 5 (3) ◽

pp. 11-20

Author(s):

Stefani Dhale Rale ◽

Hasim Hasim ◽

Syamsul Falah

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Ethanol Extract ◽

Ethyl Acetate Fraction ◽

Inhibition Activity ◽

Active Compounds ◽

Activity Inhibition ◽

Activity Test ◽

Ic50 Value ◽

Treatment Of Diabetes

This study aims to find the treatment of diabetes using natural materials by exploring plants in the province of East Nusa Tenggara. his research was conducted out by extracting the Strychnos nitida G.Don stem using a method of maceration by ethanol 70%. Ethanol extract was then fractionated using n-hexane and ethyl acetate. Simplicia from maceration and fractionation results were then tested for antioxidant activity, α-glucosidase inhibition activity and identification of active compounds. The results showed that ethyl acetate fraction had the lowest IC50 value of 86.83 μg / ml. Results of the α-glucosidase activity test showed that ethyl acetate fraction and n-heksan fraction at 900 ppm had the highest percentage of inhibition of 34.23% and 33.89%. Identification using LCMS/MS method showed that ethyl acetate fraction consist of Benzenemethamine, N, N-dioctyl- as an antioxidantcompound and compound 24-methyl-5-cholestone-hexol as an antidiabetic compound. From the results of this study, we concluded that the extract of kayu ular Strychnos nitida G.Don stem has inhibition activity toward α-glucosidase enzyme.

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Ekstraksi Protein dari Rhodophyta dan Chlorophyta Dari Perairan Pulau Pari Sebagai Alternatif Antioksidant

Jurnal Jaring SainTek ◽

10.31599/jaring-saintek.v1i1.186 ◽

2020 ◽

Vol 1 (1) ◽

pp. 24-31

Author(s):

Elvi Kustiyah ◽

Bungaran Saingin ◽

Hernowo Widodo ◽

Viriya Piti

Keyword(s):

Antioxidant Activity ◽

Low Temperature ◽

Protein Content ◽

Green Algae ◽

Red Algae ◽

Marine Resources ◽

Activity Test ◽

Ic50 Value ◽

Dpph Method ◽

Lowry Method

Indonesia has millions island and big part of Indonesia is sea that is rich in marine biological resources and has the potential to be developed and optimized. One of the abundant marine resources in Indonesia is algae. Algae are plant-like protists. Algae have several important compounds, including protein, carbohydrates, fats, minerals and other useful elements. Proteins contained in algae have the potential to be used as antioxidants. In this study, the levels of protein in red and green algae were tested by using the lowry method and testing the antioxidant activity of red and green algae extracts using the Diphenylpicrylhydrazyl (DPPH) method. Algae extraction was done by maceration, which is soaking the sample in low temperature with phosphate buffer saline (PBS) pH 7. From the extraction results it can be concluded that the red algae (Rhodophyta) has the highest protein content of 5.115 ± 0.126% and the lowest protein content in green algae (Chloropytha) as big as 1.686 ± 0.430%. And from the results of the antioxidant activity test showed that all positive algae showed antioxidant activity but the green algae (Chlorophyta) had the highest antioxidant activity of 71.5946 ± 0.01612% with IC50 value 1.6114.

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GALLIC ACID: A PHENOLIC ACID AND ITS ANTIOXIDANT ACTIVITY FROM STEM BARK OF CHLOROFORM EXTRACTS OF SYZYGIUM LITORALE (BLUME) AMSHOFF (MYRTACEAE)

Molekul ◽

10.20884/1.jm.2016.11.2.215 ◽

2016 ◽

Vol 11 (2) ◽

pp. 180 ◽

Cited By ~ 1

Author(s):

Tukiran Tukiran ◽

Fitriyatul Mahmudah ◽

Nurul Hidayati ◽

Kuniyoshi Shimizu

Keyword(s):

Antioxidant Activity ◽

Gallic Acid ◽

Phenolic Acid ◽

Methanol Extract ◽

Authentic Sample ◽

Stem Bark ◽

Spectroscopic Studies ◽

Chloroform Fraction ◽

Ic50 Value ◽

Isolated Compound

A phenolic acid had been isolated from chloroform soluble fractions of a methanol extract of stem bark of Syzygium litorale, Fam. Myrtaceae. The structure of the isolated compound was elucidated and established as gallic acid through extensive spectroscopic studies (UV-Vis, FTIR, and NMR) and by comparison with literature data and authentic sample. This is the first report of the isolation of compound from this plant, although it has previously been found in Myrtaceae family such as S. aromaticum, S. cumini, S. polyanthum, S. cordatum, etc. The chloroform fraction, isolated compound, and vitamin C showed very strong antioxidant activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) with IC50 value of 23.2, 7.5, and 12.5 mg/mL, respectively.

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Aktivitas Antioksidan Ekstrak Etanol Buah, Daun, Batang Dan Rimpang Pada Tanaman Wualae (Etlingera Elatior (Jack) R.M Smith)

Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) ◽

10.22487/j24428744.2019.v5.i2.13671 ◽

2019 ◽

Vol 5 (2) ◽

pp. 189-197 ◽

Cited By ~ 1

Author(s):

Asriullah Jabbar ◽

Wahyuni Wahyuni ◽

Muh Hajrul Malaka ◽

Apriliani Apriliani

Keyword(s):

Antioxidant Activity ◽

Vitamin C ◽

Typhoid Fever ◽

Skin Diseases ◽

Activity Test ◽

Ic50 Value ◽

Dpph Method ◽

Ic50 Values ◽

Ethanol Extracts ◽

Etlingera Elatior

Wualae (Etlingera elatior (Jack) R.M Smith) is one of the plants in the Zingiberaceae family, widely used in medicine ranging from rhizomes, fruits, and flowers. Empirically Wualae are usually used by people for treating skin diseases, flavoring food, a natural soap, and in Southeast Sulawesi, it is used to treat typhoid fever. This study aims to examine the antioxidant activity of Wualae (Etlingera elatior) plant. The plant extracts were extracted by maceration method, antioxidant activity test was performed by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) method. The results showed that ethanol extracts of fruits, leaves, stems and rhizomes of Wualae (Etlingera elatior) has the potential antioxidant activity with the IC50 values of 72.518 mg/L, 99.890 mg/L, 52.345 mg/L and 58.638 mg/L, respectively. and Vitamin C has IC50 value of 3,787 mg/L.

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The radical scavenging activity of 2-2’ diphenyl -1- picrylhydrazil (DPPH) on the methanol extracts and Ethyl acetate fractions of red dragon fruit peel (Hylocereus polyrhizus (F.A.C.Weber) Britton dan Rose)

International Journal of Phytomedicine ◽

10.5138/09750185.1945 ◽

2017 ◽

Vol 9 (1) ◽

pp. 79

Author(s):

Sri Wahdaningsih ◽

Subagus Wahyuono ◽

Sugeng Riyanto ◽

Retno Murwanti

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Methanol Extract ◽

Soluble Fraction ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Insoluble Fraction ◽

Fruit Peel ◽

Activity Test ◽

Dragon Fruit

Red dragon fruit (H. Polyrhizus) is one of the the plants that has a great potential as natural antioxidant. This study tested the activity of radical scavenging of 2-2' diphenyl -1- pikril hidrazil (DPPH) in the methanol extract, as well as in the soluble and insoluble fractions of ethyl acetate of red dragon fruit peel. This research is carried out through various stages, such as: extraction and fractionation to obtain both insoluble fraction and soluble fractions of ethyl acetate. Antioxidant activity test is conducted by the method of thin layer chromatography and spectrophotometry. Antioxidant activity test, IC50 values of methanol extract, ethyl acetate soluble fraction, and insoluble fraction of ethyl acetate had been obtained consecutively as much as 241.19 µg /mL, 8.34 µg/mL, 46.84 µg/mL. The soluble fraction of ethyl acetate had greater antioxidant activity compared to the methanol extract and the insoluble fractions of ethyl acetate.

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