scholarly journals Prevention of progression of cervical carcinogenesis at the stage of postoperative rehabilitation

2021 ◽  
Vol 6 ◽  
pp. 38-42
Author(s):  
C.M. Kartashov ◽  
T.V. Bazarinskaya
Keyword(s):  
Hyaluronic Acid ◽  
Antiviral Therapy ◽  
Plant Extracts ◽  
Comparison Group ◽  
Laser Vaporization ◽  
Low Grade ◽  
Squamous Intraepithelial Lesions ◽  
Basic Group ◽  
Flower Extract ◽  
Intraepithelial Lesions

The objective: a study the effect of a complex of hyaluronic acid with plant extracts and a polyphenolic component on cell proliferation and the progression of cervical carcinogenesis.Materials and methods. 74 patients with cervical pathology (average age is 29.8 years) formed the basic group and were examined in the clinic “Doctor Alex” (Kharkiv), 62 women of them had diathermoconization, 12 – laser vaporization of the cervix. Histologically, high-grade squamous intraepithelial lesions (HSIL) was detected in 20 patients, low-grade squamous intraepithelial lesions (LSIL) was found in 46 women, and epidermal endocervicosis – in 8 persons.The patients in the basic group during the postoperative period at the stage of healing additionally used vaginal suppositories of hyaluronic acid with plant extracts and polyphenolic component (aloe vera leaf juice, chamomile flower extract, Centella asiatica extract, sea buckthorn oil, tea tree oil, calendula flower extract, epigallocatechin gallate).The comparison group included 112 women (average age is 32.4 years), 31 of them were diagnosed HSIL, 67 – LSIL, 14 – epidermal endocervicosis; laser vaporization was performed in 21 women, diathermoconization – 91. These patients received antiviral therapy. Markers of proliferation p16 and Ki-67 and the presence of human papilloma virus (HPV) were determined in all women of both groups.Results. 6 months after the treatment, there was a decrease in the frequency of HPV in the basic group (from 55.4% to 5.4%) and in the comparison group (from 51.8% to 6.3%), proliferation markers – from 23.0% to 2.7% and from 20.5% to 6.3%, respectively. In the basic group, a more rapid epithelialization of the cervix was established, which after laser vaporization lasted 28.7 days, after diathermoconization – 36.9 days, and in the comparison group these indicators were, respectively, 30.2 and 44.6 days.Conclusions. The use of laser vaporization or diathermoconization of the cervix in the patients with background cervical pathology, LSIL and HSIL in combination with courses of vaginal suppositories containing hyaluronic acid with plant extracts and a polyphenolic component, can significantly reduce the incidence of HPV, leads to a faster recovery of its epithelium and can be comparable in the effectiveness to the combination of laser vaporization / diathermoconization with antiviral therapy.

scholarly journals Colposcopic characteristics and Lugol׳s staining differentiate anal high-grade and low-grade squamous intraepithelial lesions during high resolution anoscopy

2015 ◽  
Vol 1 ◽  
pp. 101-108 ◽  
Author(s):  
Naomi Jay ◽  
J. Michael Berry ◽  
Christine Miaskowski ◽  
Misha Cohen ◽  
Elizabeth Holly ◽  
...  
Keyword(s):  
High Resolution ◽  
Low Grade ◽  
High Grade ◽  
Squamous Intraepithelial Lesions ◽  
Intraepithelial Lesions

scholarly journals Oral contraceptive pills are associated with artifacts in ThinPrep Pap smears that mimic low-grade squamous intraepithelial lesions

Cancer ◽  
2002 ◽  
Vol 99 (2) ◽  
pp. 75-82 ◽  
Author(s):  
Carl Morrison ◽  
Patricia Prokorym ◽  
Clara Piquero ◽  
Paul E. Wakely ◽  
Gerard J. Nuovo
Keyword(s):  
Oral Contraceptive ◽  
Pap Smears ◽  
Low Grade ◽  
Squamous Intraepithelial Lesions ◽  
Oral Contraceptive Pills ◽  
Contraceptive Pills ◽  
Intraepithelial Lesions

scholarly journals Colposcopy, cervical cytology and human papillomavirus detection as screening tools for cervical cancer

2001 ◽  
Vol 7 (1-2) ◽  
pp. 100-105
Author(s):  
Al Alwan Al Alwan
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
Pap Test ◽  
Screening Tools ◽  
Low Grade ◽  
Squamous Intraepithelial Lesions ◽  
Sampling Device ◽  
Squamous Cells ◽  
The Bethesda System ◽  
Intraepithelial Lesions

A cohort of 77 women referred for routine screening or investigation of Pap test abnormality underwent colposcopic examination. Pap-stained liquid-based preparations were diagnosed and categorized according to the Bethesda system. Residual material on the sampling device was used to detect high-risk oncogenic human papillomavirus DNA. Although the colposcopic failure rate was higher than that of cytology, no lesion was missed when both methods were used together. High-risk types were recorded in 24% of patients with atypical squamous cells of undetermined significance, 45% with low-grade squamous intraepithelial lesions and 79% with high-grade squamous intraepithelial lesions-indicating that the efficacy of cytological screening can be improved by papillomavirus detection.

scholarly journals The effectiveness of acetic acid wash protocol and the interpretation patterns of blood contaminated cervical cytology ThinPrep® specimens

CytoJournal ◽  
2015 ◽  
Vol 12 ◽  
pp. 23 ◽  
Author(s):  
Nora K. Frisch ◽  
Yasin Ahmed ◽  
Seema Sethi ◽  
Daniel Neill ◽  
Tatyana Kalinicheva ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Statistical Significance ◽  
Cervical Cytology ◽  
Low Grade ◽  
Squamous Intraepithelial Lesions ◽  
Chi Square ◽  
Chi Square Test ◽  
Acid Wash ◽  
The Difference ◽  
Intraepithelial Lesions

Background: ThinPrep® (TP) cervical cytology, as a liquid-based method, has many benefits but also a relatively high unsatisfactory rate due to debris/lubricant contamination and the presence of blood. These contaminants clog the TP filter and prevent the deposition of adequate diagnostic cells on the slide. An acetic acid wash (AAW) protocol is often used to lyse red blood cells, before preparing the TP slides. Design: From 23,291 TP cervical cytology specimens over a 4-month period, 2739 underwent AAW protocol due to initial unsatisfactory smear (UNS) with scant cellularity due to blood or being grossly bloody. Randomly selected 2739 cervical cytology specimens which did not undergo AAW from the same time period formed the control (non-AAW) group. Cytopathologic interpretations of AAW and non-AAW groups were compared using the Chi-square test. Results: About 94.2% of the 2739 cases which underwent AAW were subsequently satisfactory for evaluation with interpretations of atypical squamous cells of undetermined significance (ASCUS) 4.9% (135), low-grade squamous intraepithelial lesions (LSIL) 3.7% (102), and high-grade squamous intraepithelial lesions (HSIL) 1% (28). From the 2739 control cases, 96.3% were satisfactory with ASCUS 5.5% (151), LSIL 5.1% (139), and HSIL 0.7% (19). The prevalence of ASCUS interpretations was similar (P = 0.33). Although there were 32% more HSIL interpretations in the AAW group (28 in AAW vs. 19 in non-AAW), the difference was statistically insignificant (P = 0.18). AAW category; however, had significantly fewer LSIL interpretations (P = 0.02). The percentage of UNS cases remained higher in the AAW group with statistical significance (P < 0.01). Conclusions: While AAW had a significantly higher percent of UNS interpretations, the protocol was effective in rescuing 94.2% of specimens which otherwise may have been reported unsatisfactory. This improved patient care by avoiding a repeat test. The prevalence of ASCUS and HSIL interpretations between AAW and non-AAW groups were comparable. Though not statistically significant, HSIL interpretations were relatively higher in the AAW group. LSIL interpretations showed lower prevalence in AAW group.

scholarly journals Prevalence of antibodies against a cyclic peptide mimicking the FG loop of the human papillomavirus type 16 capsid among Tunisian women

2020 ◽  
Author(s):  
Elham Hassen ◽  
Devendra Bansal ◽  
Randa Ghdira ◽  
Anouar Chaieb ◽  
Hedi Khairi ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Human Papillomavirus ◽  
Sex Workers ◽  
Synthetic Peptide ◽  
Cyclic Peptides ◽  
Cyclic Peptide ◽  
Low Grade ◽  
Squamous Intraepithelial Lesions ◽  
Intraepithelial Lesions ◽  
Peptide Antibodies

Abstract Background In the past decade, cervical cancer has gone from being the second to the fourth most common cancer in women worldwide, but remains the second most common in developing countries. This cancer is most commonly caused by high-risk types of human papillomavirus (HPV), mainly type 16 (HPV16), which are sexually transmitted. This study aimed to investigate the usefulness of a cyclic synthetic peptide designed from the major L1 capsid protein of HPV16 for detecting anti-HPV16 antibodies. Methods We designed and synthetized a peptide that corresponds to the full sequence of the surface-exposed FG loop. We tested the antigenicity of the linear and the cyclic peptides against HPV16 L1 monoclonal antibodies. We used ELISA to detect anti-peptide antibodies in sera and cervical secretions of 179 Tunisian women, and we applied polymerase chain reaction and direct sequencing methods to detect and genotype HPV DNA. Results Both the linear and the cyclic peptides were recognized by the same neutralizing monoclonal antibodies, but the cyclic peptide was more reactive with human sera. The prevalence of the anti-peptide antibodies in sera was higher in women with low-grade squamous intraepithelial lesions (LGSIL) than in women with high-grade squamous intraepithelial lesions (HGSIL) (44% and 15%, respectively). This contrasts with HPV16 DNA prevalence. Compared to women from the general population, systemic IgG prevalence was significantly higher among sex workers (25%; P=0.002) and women with LGSIL (44%; P=0.001). In addition, systemic IgA and cervical IgG prevalence was higher among sex workers only (p=0.002 and P=0.001 respectively). We did not observe anti-peptide IgG antibodies in women with a current HPV16 infection.Conclusion Anti-peptide IgG in sera or in cervical secretions could be markers of an effective natural immunization against HPV16. This may open novel perspectives for monitoring vaccinated women and for the design of synthetic peptide-based vaccines.

scholarly journals Human papillomavirus-associated anal squamous intraepithelial lesions in men who have sex with men and transgender women living with and without HIV in Karachi Pakistan: implications for screening and prevention

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Muslima Ejaz ◽  
Muhammad Mubarak ◽  
Tazeen Saeed Ali ◽  
Sören Andersson ◽  
Anna Mia Ekström
Keyword(s):  
Cox Regression ◽  
Transgender Women ◽  
Hiv Care ◽  
Low Grade ◽  
Squamous Intraepithelial Lesions ◽  
Transmitted Infection ◽  
Anal Cytology ◽  
Anal Squamous Intraepithelial Lesions ◽  
Sex With Men ◽  
Intraepithelial Lesions

Abstract Background Anal squamous intraepithelial lesions (ASIL), strongly related to human papilloma virus (HPV) infection, is more prevalent among men who have sex with men (MSM). However, no such data are available for Pakistan yet, and neither HPV vaccination nor anal-cytology screening is implemented in Pakistan. The purpose of this first ever study was to assess the prevalence of HPV-related anal cytological abnormalities among MSM and transgender women living with and without HIV infection in Pakistan. Methods We conducted a cross-sectional study from March 2016 to November 2017 at sexual health centers run by the Perwaaz Trust and the National AIDS Control Program in Karachi. The study enrolled MSM and transgender women aged greater-than-and-equal-to-18-years who reported anal sex in the preceding 6 months. We collected two anal samples for liquid-based cytology and HPV type testing by PCR, and socio-demographic and behavioral data were collected through face-to face interviews. ASIL and its associations with biological and behavioral risk factors were analyzed through Cox regression for prevalence ratios (PR) and corresponding 95% confidence intervals (CIs). Results Out of 271 qualifying participants, 79% were MSM and 21% transgender women. The mean age was 28.8 (± 8) years. Almost 35% (93/271) of the study population had ASIL detected, ASIL was significantly more common among participants living with HIV than in HIV negative ((50/118) 42.4%; vs. (43/153) 28.1%) (p ≤ 0.001). Among ASIL, 66% (61/93) had low-grade squamous intraepithelial lesions (LSIL), and 3.6% (3/93) had high-grade squamous intraepithelial lesions (HSIL). The overall, HPV16 positivity was 35.5% (33/93) among all abnormal anal lesions and all 3 HSIL were HPV16 positive, however, HPV16 positivity could show its association with ASIL detection in univariate model only (PRcrude: 2.11(1.39–3.18)). Moreover, any HR-HPV type (PR 3.04; 95% CI 1.75–5.26), concurrent sexually transmitted infection (STI) (2.13; (1.28–3.55)) and HIV + /HPV + coinfection (1.75; (1.07–2.88)) remained independently associated with ASIL in the multivariate model. Conclusions Abnormal anal cytology among MSM and transgender is prevalent enough to consider optimal screening regimens. Further studies are required to see if periodic anal cytology can be made part of HIV care and treatment programs among MSM in Pakistan.

scholarly journals Multiple types of human papillomavirus infection and anal precancerous lesions in HIV-infected men in Taiwan: a cross-sectional study

BMJ Open ◽  
2018 ◽  
Vol 8 (1) ◽  
pp. e019894 ◽  
Author(s):  
Shu-Hsing Cheng ◽  
Kuo-Sheng Liao ◽  
Chi-Chao Wang ◽  
Chien-Yu Cheng ◽  
Fang-Yeh Chu
Keyword(s):  
Human Papillomavirus ◽  
Cross Sectional Study ◽  
Low Grade ◽  
Sectional Study ◽  
Squamous Intraepithelial Lesions ◽  
Cross Sectional ◽  
Anal Cytology ◽  
Hpv Types ◽  
The Relationship ◽  
Intraepithelial Lesions

ObjectivesThis study aimed to assess the relationship between infection with multiple human papillomavirus (HPV) types and abnormal anal cytology in HIV-infected men.DesignAn observational, cross-sectional study.SettingA regional referral hospital in Taiwan.ParticipantsIn total, 714 HIV-infected men were enrolled between March 2011 and June 2016. Thin preparation anal Pap smears were interpreted according to the 2001 Bethesda System. Thirty-seven types of HPV were detected by reverse line blotting, including 13 oncogenic types and 24 non-oncogenic types.Outcome measuresThe relationship between anal HPV infection and abnormal anal cytology in people of Asian ethnicity and the coverage efficacy in HPV-vaccinated HIV-infected men.ResultsOn anal cytology, 175 (24.5%) subjects had atypical squamous cells of undetermined significance (ASCUS) or higher grades of dysplasia, including 87 (49.7%) with ASCUS, 73 (41.7%) with low-grade squamous intraepithelial lesions (LSILs) and 15 (8.6%) with high-grade squamous intraepithelial lesions (HSILs). A higher proportion of subjects with those without LSIL/HSIL (93.1% vs 67.3%, P<0.0001) had multiple HPV types. The odds of having LSIL/HSIL increased with an increasing number of HPV types: the ORs ranged from 1 for no HPV types to 6.96 (95% CI 2.38 to 20.37) for more than five types (Ptrend<0.0001). Multivariate logistic regression analysis showed a significant association between LSIL/HSIL and the number of HPV genotypes present (OR 1.20; 95% CI 1.02 to 1.42, P<0.05). HPV types covered by the nonavalent HPV vaccine (types 6/11/16/18/31/33/45/52/58) were detected in 70.1% of the patients in this study.ConclusionsThe odds of having anal LSIL/HSIL are approximately seventimes greater in HIV-infected men with than withoutsix or more types of HPV. Multiple HPV types in HIV-infected patients deserves aggressive follow-up, and HPV vaccination programme require scaling up.

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