scholarly journals Assessment of anti bacterial, anti inflammation and wound healing activity in Wistar albino rats using green silver nanoparticles synthesized from Tagetes erecta leaves

2021 ◽  
Vol 13 (1) ◽  
pp. 343-351
Author(s):  
S. IruthayaKalai Selvam ◽  
S. Marian Bara Joicesky ◽  
A. Amolorpava Dashli ◽  
A. Vinothini ◽  
K. Premkumar
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Wound Healing ◽  
Silver Nanoparticles ◽  
Albino Rats ◽  
Ag Nps ◽  
Anti Inflammatory ◽  
Wistar Albino Rats ◽  
Wound Healing Activity ◽  
Scanning Electron

Silver nanoparticles synthesized from plant material have superior bioactivities. The purpose of this current study was to synthesis, characterize and to explore the bioactive efficacy of silver nanoparticles (Ag-NPs) using aqueous leaf extract of Tageteserecta. The biosynthesized Ag-NPs were characterized using ultraviolet-visible spectroscopy, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction and Scanning electron microscopy. Ag-NPs were studied for in-vivo anti-inflammatory and wound healing activities performed in female Wistar albino rats. UV –Vis absorption spectrum of the T.erecta leaves extract was obtained at 428nm due to excitation of surface plasmon vibration in nanoparticles and confirms the synthesis of silver nanoparticles. The FTIR analysis showed the presence of sulfate, alkene and alcohol in the AgNP of T.erectaleaves. The average crystallite size of AgNP synthesized was found to be 27.2 nm. The spherical silver grain of 15.5 nm average size has been depicted with high-resolution scanning electron microscopy. Maximum activity (15mm) of T.erecta leaves silver nanoparticles was observed against Salmonella typhi (15mm) followed by Escherichia coli (12mm). Ag-NPs exhibited significant wound healing activity and anti-inflammatory activity in carrageenan-induced paw volume tests performed in female Wistar albino rats. Colloidal Ag-NPs can be synthesized by simple, nonhazardous methods, and biosynthesized Ag-NPs using T.erectaleaves extract have significant therapeutic properties.This work evidently confirmed that silver nanoparticles mediated T.erecta could be considered as a potential source for anti-inflammatory and wound healing drug.

Loading AKBA on surface of silver nanoparticles to improve their sedative-hypnotic and anti-inflammatory efficacies

Nanomedicine ◽  
2019 ◽  
Vol 14 (21) ◽  
pp. 2783-2798
Author(s):  
Ajmal Khan ◽  
Ahmed Al-Harrasi ◽  
Najeeb Ur Rehman ◽  
Rizwana Sarwar ◽  
Touqeer Ahmad ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Silver Nanoparticles ◽  
Water Solubility ◽  
Boswellic Acid ◽  
Transmission Electron ◽  
Anti Inflammatory ◽  
Compound Materials ◽  
Dose Dependent ◽  
Scanning Electron

Aim: Acetyl-11-keto- β-boswellic acid (AKBA) is a potent anti-inflammatory compound limited by its low water solubility and bioavailability. To load AKBA on silver nanoparticles (AgNPs) to improve bioavailability and water solubility of the compound. Materials & methods: AKBA-AgNPs were chemically synthesized and characterized by UV–Vis spectrophotometry, Fourier transform infrared spectroscopy, scanning electron microscopy and transmission electron microscopy. AKBA and AKBA-Ag were studied for their sedative-hypnotic and anti-inflammatory efficacies. Results: Pretreatment with AKBA or AKBA-Ag caused significant dose-dependent sedative-hypnotic effects at 5 and 10 mg/kg intraperitoneal. The effects of AKBA-loaded AgNPs caused pronounced changes in mice compared with those of AKBA, and the AKBA-AgNPs demonstrated anti-inflammatory effects that were superior to those of AKBA. Conclusion: The loading of AKBA on nanoparticles improved its pharmacokinetic effects, and capacity for drug delivery.

scholarly journals Silver Nanoparticles: Green Synthesis, Characterization, Blood Compatibility and Protoscolicidal Efficacy against Echinococcus granulosus

2021 ◽  
Vol 41 (03) ◽  
pp. 393-399
Author(s):  
Parwin Jalal Jalil
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Silver Nanoparticles ◽  
Echinococcus Granulosus ◽  
Blood Compatibility ◽  
Ag Nps ◽  
X Ray ◽  
Hydatid Fluid ◽  
Scanning Electron

Spillage of protoscoleces within hydatid fluid during surgery for hydatid cyst is the main reason for its recurrence. Therefore, to inactivate the protoscoleces, various scolicidal substances have been tested. However, novel and more efficient agents are needed owing to several associated complications. This study focused on the effects of green synthetic Silver Nanoparticles (AgNPs) from Zizyphus spina- christi leaves on Echinococcus granulosus protoscoleces. Also, to evaluate the blood compatibility of Ag NPs. The Ag NPs were identified by ultraviolet-visible (UV-Visible) spectrophotometer, X-ray diffraction (XRD), Scanning electron microscopy imaging, and Energy-dispersive X-ray spectroscopy (EDX). Hydatid fluid was aspirated aseptically from cysts of infected sheep liver. The protoscoleces were exposed to Ag NPs at several concentrations. Also, scanning electron microscopy for ultrastructural changes and in vitro erythrocytes lysis was performed. The Ag NPs were spherical; the particles' size reached 50 nm, and presented a surface plasmon peak around 460 nm. The current study's findings indicated the powerful in vitro scolicidal efficacy of the green biosynthesized AgNPs. Several morphological alterations were observed on the protoscoleces by optical and scanning electron microscopy. Lysis of RBCs at different doses of Ag NPs was significantly (P≤0.05) less than the positive control value, thus proposing its biocompatibility. This work suggests that chemicals like polyphenols present in the extract of Z. spina- christi act as reducing and stabilizers agents to create Ag NPs Nevertheless, further investigations are needed to investigate the Ag NPs scolicidial effects in animal models.

scholarly journals Synthesis of silver nanoparticles using marine macroalgae Padina sp. and its antibacterial activity towards pathogenic bacteria

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Prakash Bhuyar ◽  
Mohd Hasbi Ab. Rahim ◽  
Sathyavathi Sundararaju ◽  
Rameshprabu Ramaraj ◽  
Gaanty Pragas Maniam ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Marine Algae ◽  
Marine Alga ◽  
Ag Nps ◽  
X Ray ◽  
Uv Visible ◽  
Scanning Electron

Abstract Background Marine algae used as a food source for ocean life and range in color from red to green to brown grow along rocky shorelines around the world. The synthesis of silver nanoparticles by marine alga Padina sp. and its characterization were fulfilled by using UV-visible spectrophotometer, Fourier transform infrared spectroscopy, scanning electron microscopy and field emission scanning electron microscopy, and energy-dispersive X-ray spectroscopy. Results UV-visible absorption spectrum revealed that the formation of Ag nanoparticles was increased by the addition of marine algae and the spectral peak observed between a wavelength of ~ 420 nm and 445 nm. In addition, SEM and FESEM images examined the surface morphology and the size of the synthesized NPs was relatively uniform in size ~ 25–60 nm. Energy-dispersive X-ray spectroscopy analysis confirmed the purity of Ag NPs with atomic percentage of 48.34% Ag. The synthesized Ag NPs showed highly potent antibacterial activity. The Staphylococcus aureus and Pseudomonas aeruginosa were found to be more susceptible to silver nanoparticles by forming 15.17 ± 0.58 mm and 13.33 ± 0.76 mm of diameter of the inhibition zone, respectively. Conclusions The study suggested that marine alga Padina sp. could be an alternative source for the production of Ag nanoparticles and are efficient antimicrobial compounds against both gram-negative and gram-positive bacteria which can be a promising material against infectious bacteria.

An in vitro study on the burn wound healing activity of cotton fabrics incorporated with phytosynthesized silver nanoparticles in male Wistar albino rats

2017 ◽  
Vol 100 ◽  
pp. 187-196 ◽  
Author(s):  
Balashanmugam Pannerselvam ◽  
Mukesh Kumar Dharmalingam Jothinathan ◽  
Murugan Rajenderan ◽  
Palani Perumal ◽  
Kalaichelvan Pudupalayam Thangavelu ◽  
...  
Keyword(s):  
Wound Healing ◽  
Silver Nanoparticles ◽  
In Vitro Study ◽  
Cotton Fabrics ◽  
Albino Rats ◽  
Burn Wound ◽  
Burn Wound Healing ◽  
Wistar Albino Rats ◽  
Wound Healing Activity

scholarly journals Enhancement of Photoconductive Detector Based on Carbon Nanotubes Decorated with Silver Nanoparticles by Adding Conductive Polymer

2021 ◽  
Vol 19 (50) ◽  
pp. 84-93
Author(s):  
Taqwa Yousif ◽  
Asama Naje
Keyword(s):  
Electron Microscopy ◽  
Carbon Nanotubes ◽  
Scanning Electron Microscopy ◽  
Silver Nanoparticles ◽  
Forward Bias ◽  
X Ray Diffraction ◽  
Ag Nps ◽  
X Ray ◽  
Vis Spectroscopy ◽  
Scanning Electron

In this work, wide band range photo detector operating in UV, Visible and IR was fabricated using carbon nanotubes (MWCNTs, SWCNTs) decorated with silver nanoparticles (Ag NPs). Silicon was used as a substrate to deposited CNTs/Ag NPs by the drop casting technique. Polyamide nylon polymer was used to coat CNTs/Ag NPs to enhance the photo-response of the detector. The electro-exploding wire technology was used to synthesize Ag NPs. Good dispersion of silver NPs achieved by a simple chemistry process on the surface of CNTs. The optical, structure and electrical characteristic of CNTs decorated with Ag NPs were characterized by X-Ray diffraction and Field Emission Scanning Electron Microscopy.  X-ray diffraction patterns of Ag NPs exhibited 2θ values (38.1°,44.3°) corresponding to the Ag nanocrystal, while the XRD pattern of MWCNTs and SWCNTs /Ag NPs peaks appeared at 2θ = 26.2° corresponding to the (002) and at 2theta=44° which corresponds with miller indices (100) for CNTs and (200) for Ag NPs. The optical properties measured by UV-Vis. Spectroscopy. Broad and strong surface plasmon resonance (SPR) peak was detected at 420 nm, for Ag NPs. The absorption of CNTs/Ag NPs increased significantly from UV to near IR region (300-1000 nm).  Ag NPs decorated CNTs without any impurities, according to field mission scanning electron microscopy examination, with typical particle sizes of (50-80nm) for Ag-NPs, 44nm for MWCNTs/Ag-NPs, and 30nm for SWCNTs/Ag NPs. ֹThe I-V characteristics at forward bias voltage (0.5-10) volt were studied. The figure of merits (responsivity, photocurrent gain, NEP and detectivity) after coating with polymer of the detector were measured in the dark and after illumination with UV LED (365 nm), Tungsten lamp (500-800 nm) and Laser diode (808 nm).

scholarly journals GREEN TECHNOLOGY MEDIATED SYNTHESIS OF SILVER NANOPARTICLES FROM MOMORDICA CHARANTIA FRUIT EXTRACT AND ITS BACTERICIDAL ACTIVITY

2017 ◽  
Vol 10 (3) ◽  
pp. 196 ◽  
Author(s):  
S C Joshi ◽  
Utkarsh Kaushik ◽  
Aproova Upadhyaya ◽  
Priyanka Sharma
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Silver Nanoparticles ◽  
Atomic Force Microscope ◽  
Momordica Charantia ◽  
Diffusion Method ◽  
Fruit Extract ◽  
Atomic Force ◽  
Vis Spectroscopy ◽  
Scanning Electron

ABSTRACTObjective: The synthesis of nanoparticles from biological processes is evolving a new era of research interests in nanotechnology. Silver nanoparticlesare usually synthesized by chemicals and physical method, which are quite toxic and flammable in nature. This study deals with an environmentfriendly biosynthesis process of antibacterial silver nanoparticles using Momordica charantia fruit.Methods: AgNO3 (5 mM) was allowed to react with fruit extract of M. charantia. Biosynthesis of AgNPs was optimized by changing temperature,pH, and solvent. The silver nanoparticles so formed were characterized using ultraviolet-visible (UV-VIS) spectroscopy, Fourier transform infraredspectroscopy (FTIR), dynamic light scattering (DLS), atomic force microscope (AFM), and scanning electron microscopy (SEM).Results: UV-VIS spectra show absorption peak between 420 and 430 nm. The FTIR analysis showed the alcoholic, lactam, and nitro group presentin the plant extract, which were responsible for the reduction in AgNPs. The SEM images showed the size distribution of the nanoparticles and theaverage size was found to be 50-100 nm. By DLS analysis and AFM analysis, average sizes of the silver nanoparticles were of 150 nm. The results ofthese analyses confirmed the formation of silver nanoparticles. Silver nanoparticles were tested against Bacillus cereus and Staphylococcus epidermidisstrains using disc diffusion method and were found to be effective.Conclusion: Silver nanoparticles so synthesized in this study using fruit extract of M. charantia are simple, easy, and effective technique of nanoparticlesproduction.Keywords: Silver nanoparticles, Momordica charantia, Optimization, Antibacterial, Atomic force microscope, Scanning electron microscopy.

scholarly journals EVALUATION OF WOUND HEALING ACTIVITY OF METHANOLIC EXTRACT OF SMILAX WIGHTII (A. DC.) IN WISTAR ALBINO RATS

2014 ◽  
Vol 1 (1) ◽  
pp. 73-77
Author(s):  
Uma Maheswari P ◽  
Shalimol A ◽  
Arumugasamy A ◽  
Udhaya Sankar M.R
Keyword(s):  
Wound Healing ◽  
Dry Weight ◽  
Wound Contraction ◽  
Albino Rats ◽  
Standard Drug ◽  
Excision Wound ◽  
Wound Model ◽  
Wistar Albino Rats ◽  
Incision Wound ◽  
Wound Healing Activity

The present experimental study was designed to evaluate the wound healing activity of methanolic extract of Smilax wightii A. DC. on incision and excision wound models in Wistar albino rats. The parameters studied were wound breaking strength, wound contraction area, epithelialization period, granulation tissuewet, dry weight and hydroxyproline content in incision wound model, percentage of wound contraction and period of epithelialization in excision wound model. The rats were administered topically with 100mg/kg b.wt. (low dosage), 200mg/kg b.wt. (moderate dosage) and 500mg/kg b.wt. (high dosage) of methanolicextract of Smilax wightii (MESW). The activity of the extract treated groups were compared with that of the control 1% Spirit. Framycetin sulphate 0.2% w/w was used as the standard drug. In incision wound model, there was a significant increase in the wound breaking strength in all the experimental groups treated withMESW than that of the control. Similarly, significant (P<0.001) decrease in wound contraction area and period of epithelialization were also observed in the test group animals treated with MESW and the standard drug treated groups when compared to that of the control. , a significant increase was observed in granulation tissue wet and dry weight and hydroxyproline content in the test groups treated with MESW compared to the control. In exicision wound model, there was a significant increase (P<0.01) in the percentage of wound contraction and decrease in period of epithelialization in the experimental groups treated with 200mg/kgb.wt. (moderate dosage) and 500mg/kgb.wt (high dosage) of MESW. The extract treated groups showed significant improvement in all the wound healing parameters of incision, and excision wound models.

scholarly journals HIBISCUS ROSA SINENSIS LOADED SOLID LIPID NANOPARTICLES AND IN VIVO WOUND HEALING ACTIVITY IN WISTAR ALBINO RATS

2020 ◽  
pp. 78-83
Author(s):  
VIJAYANAND P. ◽  
JYOTHI V. ◽  
MOUNIKA A.
Keyword(s):  
Wound Healing ◽  
Solid Lipid Nanoparticles ◽  
Lipid Nanoparticles ◽  
Albino Rats ◽  
Herbal Extracts ◽  
Solid Lipid ◽  
Hibiscus Rosa Sinensis ◽  
Wistar Albino Rats ◽  
Wound Healing Activity

Objective: The objective of the present research was to investigate the wound-healing potency of solid lipid nano particles of Hibiscus rosa sinensis extract. Crude herbal extracts and rudimentary formulations containing herbal extracts are good for demonstrating the feasibility of the concept; however, such formulations suffer with poor oral bioavailability and variability within groups of subjects. Converting herbal extracts into novel drug delivery systems may prove effective in addressing some of these problems. Methods: In the present study an attempt was made to develop Hibiscus rosa sinensis extract loaded solid lipid nanoparticles (HSLNs) using lipids glycerol monostearate (GMS) or beeswax. The prepared HSLNs were characterised for their size, surface charge and morphology. The optimized HSLNs were incorporated into Carbopol gel and tested for wound healing activity in male Wistar albino rats using excision wound model. Results: HSLNs of ~175 nm in size carrying negative charge were obtained with the optimised procedure using beeswax. The shape of the HSLNs was nearly spherical. The HSLNs (10 mg/ml) treated wounds healed much faster compared to raw crude extract and healing was comparable to marketed preparation. Conclusion: It is concluded that converting crude herbal extracts into SLNs can be an effective way to enhance the effectiveness of herbal extracts and their in vivo activity.

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