The Evaluation of Trichoderma consortia against Fusarium udum causing  wilt of Pigeonpea

Pigeonpea is one of the important pulse crop of Madhya Pradesh, a State of India. The plant gets infected by the pathogen Fusarium udum causing wilt disease, which is one of the major constraints in the production and productivity of pigeonpea. The present study aimed to carry out in vitro condition to assess the possible use of biocontrol consortia in field conditions. Six Trichoderma consortia viz., T1- T. viride + T. harzianum (JC-I), T2- T. viride + T. virens (JC-2), T3:- T. harzianum +T. virens (JC-3), T4- T. hamatum +T. viride (JC-4), T5- T. hamatum + T. harzianum (JC-5), T6- T. hamatum + T. virens (JC-6) and T0- Control were evaluated for their antagonistic activity against F. udumunder in vitro conditions. The consortia of T4- T. hamatum +T. viride (JC-4) was found most effective(58.82 %) in inhibiting the radial growth of Fusarium udum. The volatile compound from consortium of T5-T. hamatum + T. harzianum (JC-5) exhibited maximum growth inhibition (81.84%) and sporulation of Fusarium udum followed by T1-T. viride + T. harzianum (JC-I) (55.49% inhibition) . The culture filtrate of consortia of T4- T. hamatum +T. viride (JC-4) showed 100% inhibition of test pathogen followed by T5-T. hamatum + T. harzianum (JC-5) (82.89%) at 5 % concentration. It was also observed that with an increase in the concentration of culture filtrates of all the Trichoderma species, the radial mycelial growth of the test pathogen was proportionally decreased. The Trichodema consortium viz., T4- T. hamatum +T. viride (JC-4) may be tried in the field to manage wilt of pigeonpea because they worked synergistically and gave the high impact of their use.

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Management of collar rot disease in chickpea by Trichoderma species

Journal of AgriSearch ◽

10.21921/jas.v7i03.18694 ◽

2020 ◽

Vol 7 (03) ◽

Author(s):

PREM PANDEY ◽

G. C. SAGAR ◽

SUNDARMAN SHRESTHA2 ◽

HIRAKAJI MANANDHAR ◽

RITESH K. YADAV ◽

...

Keyword(s):

Growth Inhibition ◽

Mycelial Growth ◽

Sclerotium Rolfsii ◽

Maximum Growth ◽

Pot Experiment ◽

Trichoderma Spp ◽

Trichoderma Species ◽

Collar Rot ◽

Rot Disease

Nine isolates of Trichoderma spp. were isolated from different agro- ecological regions of Nepal viz; Jumla, Palpa, Chitwan, Tarahara, Banke, Illam and Salyan and screened against Sclerotium rolfsii Sacc. Adreded soil borne phytopathogen causing collar rot of chickpea in chickpea; In-vitro efficacy of nine fungal antagonist (Trichoderma spp.) against Sclerotium rolfsii were screened. Pot experiment was done to find out the effective management of S. rolfsi through Tricoderma using different methods i.e. Seed treatment, soil drenching and soil application. All the tested isolates of Trichoderma spp. were found effective on mycelial growth inhibition and sclerotial parasitization of S. rolfsii. Trichoderma isolated from Palpa district showed maximum growth inhibition (%) of pathogen periodically after 48(93.78%), 72(96.00%), 96(97.96%) and 120(100.00%) hours of inoculation. Parasitized sclerotium showed minimum sclerotial germination on agar plates. Moreover, Trichoderma species isolated from Palpa districts showed second best percent mycelial growth inhibition periodically at 72(25.00%), 120(29.16%), 168(29.16%) and 216(29.16%).In pot experiment at 40 days after sowing, Seedling height was maximum in soil drenching with 30g per 100ml of water (22.27cm) and Mortality percentage of seedlings was least or highest disease control was observed in seed treated with 109cfu/ml (0.000%).

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Bio-efficacy of Trichoderma species against Pigeonpea wilt pathogen

Journal of Applied and Natural Science ◽

10.31018/jans.v9i4.1531 ◽

2017 ◽

Vol 9 (4) ◽

pp. 2327-2331 ◽

Cited By ~ 1

Author(s):

Balkishan Chaudhary ◽

Sanjeev Kumar ◽

Shiva Kant Kushwaha

Keyword(s):

Volatile Compounds ◽

Trichoderma Harzianum ◽

Mycelial Growth ◽

Biocontrol Agent ◽

Trichoderma Viride ◽

Control Agent ◽

Trichoderma Virens ◽

Trichoderma Species ◽

Fusarium Udum

Three biocontrol agent viz., Trichoderma viride, Trichoderma virens and Trichoderma harzianum were evaluated to test the antagonism against Fusarium udum under in vitro conditions. All the three biocontrol agents have the potential of parasitizing the growth of Fusarium udum in vitro. The rate of parasitism was found fastest in T. viride (61.12% over growth in 96 hrs) than T. virens and T. harzianum. The volatile compounds from Trichoderma viride suppressed the mycelial growth of Fusarium udum by 43.13% and found effective when compared to Tricho-derma virens and Trichoderma harzianum. Non-volatile compounds or culture filtrate from Trichoderma virens at 15% concentration shows complete mycelial inhibition of the test fungi. The antagonist T. virens was chosen to be the most promising bio-control agent for F. udum.

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Effects of Trichoderma Species on the Growth of Fusarium Verticillioides

Bangladesh Journal of Botany ◽

10.3329/bjb.v50i2.54101 ◽

2021 ◽

Vol 50 (2) ◽

pp. 423-425

Author(s):

Ramesh Kumar ◽

Sanjeev Kumar ◽

Balkishan Chaudhary

Keyword(s):

Trichoderma Harzianum ◽

Mycelial Growth ◽

Fusarium Verticillioides ◽

Trichoderma Viride ◽

Maximum Growth ◽

Culture Technique ◽

Dual Culture ◽

Trichoderma Virens ◽

Trichoderma Species

Six biocontrol treatments viz., Trichoderma viride, Trichoderma virens, Trichoderma harzianum, T. harzianum + T. viride, T. harzianum + T. virens and T. viride + T. virens were evaluated to test the antagonism against Fusarium verticillioides under in vitro conditions. The maximum growth inhibition (90.6%) was recorded in consortium of T. harzianum + T. viride in dual culture technique . The volatile and non volatile compounds from the consortium of T. harzianum + T. viride also found best and suppressed the mycelial growth of F. verticillioides to the tune of 83.90 and 84.61 %, respectively. Bangladesh J. Bot. 50(2): 423-425, 2021 (June)

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Management of wilt and dry root rot diseases of redgram [Cajanus cajan (L.) Millsp.] by using actinomycetes

INTERNATIONAL JOURNAL PLANT SCIENCES ◽

10.15740/has/ijps/15.2/96-100 ◽

2020 ◽

Vol 15 (2) ◽

pp. 96-100

Author(s):

S. Malathi

Keyword(s):

Root Rot ◽

Cajanus Cajan ◽

Mycelial Growth ◽

Growth Parameters ◽

Macrophomina Phaseolina ◽

Antagonistic Activity ◽

Fusarium Udum ◽

Root Rot Disease ◽

Rot Disease

Actinomycetes were tested for their antagonistic activity against Fusarium udum and Macrophomina phaseolina under in vitro condition. Among the tested isolates of Actinomycetes AC (5) reported highest 82.85 per cent reduction of mycelial growth of Fusarium udum and 85.13 per cent reduction of mycelial growth of Macrophomina phaseolina. In the field experiment, five treatments were tested for the management of wilt and dry root rot disease, T3- ST+ SA with Actinomycetes (AC 5) significantly recorded 71.92 and 70.38 per cent reduction of the wilt and dry root rot diseases, respectively. These biocontrol agents were used an alternative to the chemical fungicide for controlling the wilt and dry root rot incidence and enhanced the plant growth parameters and there by increased yield in redgram.

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Biocontrol potential of Trichoderma harzianum in controlling wilt disease of pistachio caused by Verticillium dahliae

Journal of Plant Protection Research ◽

10.1515/jppr-2017-0025 ◽

2017 ◽

Vol 57 (2) ◽

pp. 185-193 ◽

Cited By ~ 5

Author(s):

Zeinab Fotoohiyan ◽

Saeed Rezaee ◽

Gholam Hosein Shahidi Bonjar ◽

Amir Hossein Mohammadi ◽

Mohammad Moradi

Keyword(s):

Verticillium Dahliae ◽

Trichoderma Harzianum ◽

Pathogenic Fungus ◽

Antagonistic Activity ◽

Wilt Disease ◽

Dual Culture ◽

Volatile Metabolites ◽

Biocontrol Potential

Abstract Verticillium wilt caused by Verticillium dahliae, is one of the most devastating diseases in pistachio orchards in the world including Iran. In search for an effective non-chemical strategy for the management of this disease, we evaluated the biocontrol potential of Trichoderma harzianum isolates obtained from the rhizosphere of healthy pistachio trees in different locations of the Kerman province of Iran against V. dahliae under laboratory and greenhouse conditions. Dual culture tests in the laboratory were conducted in a completely randomized design using 72 T. harzianum isolates. Twenty isolates showed the highest in vitro antagonistic activity. The results indicated that all 20 isolates were capable of inhibiting the mycelial growth of V. dahliae significantly. Among them, isolates Tr8 and Tr19 were the most effective by 88.89% and 85.12% inhibition, respectively. Extracted cell free metabolites of all effective isolates also inhibited the growth of V. dahliae in the culture medium significantly. According to the results, isolates Tr4 and Tr6 inhibited fungal pathogen growth by 94.94% and 88.15% respectively, through production of non-volatile metabolites. In the evaluation of volatile metabolites, isolates Tr5 and Tr4 were the most effective by 26.27% and 24.49% growth inhibition, respectively. Based on the results of the in vitro experiments, the five most effective isolates were selected for evaluation under greenhouse conditions for their biocontrol potential in controlling Verticillium wilt of pistachio. Results of the greenhouse, (in vivo) experiments were positive and indicated that the occurrence of wilt disease in plants treated with the antagonists alone or in combination with pathogenic fungus was lower than in plants inoculated with pathogen alone. The overall results of this study suggest that Trichoderma fungal antagonist may be an effective biocontrol agent for the control of Verticillium wilt of pistachio.

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PROSPECÇÃO E AVALIAÇÃO DO POTENCIAL BIOTECNOLÓGICO DE LINHAGENS COMERCIAIS DE SHIITAKE

ENERGIA NA AGRICULTURA ◽

10.17224/energagric.2020v35n4p640-649 ◽

2021 ◽

Vol 35 (4) ◽

pp. 640-649

Author(s):

Dalvan Pereira Abilio ◽

Olívia Gomes Martins ◽

Giovana Silva ◽

Meire Andrade

Keyword(s):

Wheat Bran ◽

Radial Growth ◽

Mycelial Growth ◽

Culture Medium ◽

Lentinula Edodes ◽

Culture Media ◽

Biotechnological Potential ◽

E Mail ◽

Eucalyptus Sawdust

PROSPECÇÃO E AVALIAÇÃO DO POTENCIAL BIOTECNOLÓGICO DE LINHAGENS COMERCIAIS DE SHIITAKE DALVAN PEREIRA ABÍLIO1, OLÍVIA GOMES MARTINS2, GIOVANA CRISTINA PINTO ALVES DA SILVA3, MEIRE CRISTINA NOGUEIRA DE ANDRADE4 1 Graduando em Ciências Biológicas, Centro Universitário Sagrado Coração – Unisagrado, R. Irmã Arminda, 10-50 - Jardim Brasil, 17011-160 Bauru, SP, Brasil. E-mail: [email protected] 2 Doutoranda em Agronomia – Energia na Agricultura, Faculdade de Ciências Agronômicas, Universidade Estadual Paulista "Julio de Mesquita Filho" (UNESP), Av. Universitária, 3780 - Altos do Paraíso, 18610-034, Botucatu, SP, Brasil. E-mail: [email protected] 3 Mestre em Agronomia – Energia na Agricultura, Faculdade de Ciências Agronômicas, Universidade Estadual Paulista "Julio de Mesquita Filho" (UNESP), Av. Universitária, 3780 - Altos do Paraíso, 18610-034, Botucatu, SP, Brasil. E-mail: [email protected] 4 Docente da Faculdade Gran Tietê, Av. 15 de Novembro, 125 - Centro, 17340-000, Barra Bonita, SP, Brasil. E-mail: [email protected] RESUMO: O objetivo deste trabalho foi avaliar e comparar o crescimento micelial in vitro de quatro linhagens comerciais de Lentinula edodes (LE-241, LE-242, LE-243 e LE-244) em meio de cultura à base de serragem de eucalipto, suplementado com bagaço de malte ou farelo de trigo. O delineamento experimental foi inteiramente casualizado, em esquema fatorial 2x4 (substratos x linhgens), totalizando oito tratamentos, cada um com cinco repetições. Os substratos foram preparados com 20% de suplementação, acrescidos de calcário (tamponante), umidificados e esterilizados. Os meios de cultura foram preparados a partir da infusão dos substratos, filtração, adição de ágar e esterilização. As placas foram inoculadas com as linhagens e o crescimento radial do micélio na superfície do meio de cultura foi mensurado com paquímetro. O menor desenvolvimento micelial ocorreu com a linhagem LE-241 e com a linhagem LE-243 no substrato suplementado com farelo de trigo. Os maiores desenvolvimentos foram obtidos com as linhagens LE-242 e LE-244 no substrado suplementado com bagaço de malte. O desenvolvimento de todas as linhagens foi maior no substrato suplementado com bagaço de malte. Sendo assim, a utilização do bagaço de malte para o crescimento micelial é uma alternativa viável à suplementação tradicional com farelo de trigo. Palavras-chave: fungos, cogumelo, Lentinula edodes, micélio, bagaço de malte. PROSPECTING AND EVALUATING THE BIOTECHNOLOGICAL POTENTIAL OF SHIITAKE COMMERCIAL STRAINS ABSTRACT: This study aimed to evaluate and compare the in vitro mycelial growth of four commercial Lentinula edodes strains (LE-241, LE-242, LE-243 and LE-244) in a culture medium based on eucalyptus sawdust, supplemented with malt bagasse or wheat bran. The experimental design was completely randomized, in a 2x4 factorial scheme (substrates x strains), totaling eight treatments, each with five repetitions. The substrates were prepared with 20% supplementation, added with lime (buffer), humidified and sterilized. The culture media were prepared from the infusion of substrates, filtration, addition of agar and sterilization. The plates were inoculated with the strains and the radial growth of the mycelium on the surface of the culture medium was measured using a caliper. The smallest mycelial growth occurred with the LE-241 strain and with the LE-243 strain on the substrate supplemented with wheat bran. The greatest growths were obtained with the LE-242 and LE-244 strains in the substrate supplemented with malt bagasse. The development of all strains was greater in the substrate supplemented with malt bagasse. Therefore, the use of malt bagasse for mycelial growth is a viable alternative to traditional wheat bran supplementation. Keywords: fungi, mushroom, Lentinula edodes, mycelium, malt bagasse.

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PENGENDALIAN PENYAKIT LAYU BAKTERI NILAM MENGGUNAKAN PSEUDOMONAD FLUORESEN

Jurnal Penelitian Tanaman Industri ◽

10.21082/jlittri.v11n1.2005.19-24 ◽

2020 ◽

Vol 11 (1) ◽

pp. 19

Author(s):

NASRUN NASRUN ◽

CHRISTANTI CHRISTANTI ◽

TRIWIDODO ARWIYANTO ◽

IKA MARISKA

Keyword(s):

Plant Growth ◽

Bacterial Wilt ◽

Block Design ◽

Antagonistic Activity ◽

Wilt Disease ◽

Plant Production ◽

Fluorescent Pseudomonad ◽

Bacterial Wilt Disease ◽

Pogostemon Cablin

ABSTRAK Penelitian pengendalian penyakit layu bakteri nilam (Ralstonia solanacearum) menggunakan pseudomonad fluoresen di kebun petani nilam Desa Situak Pasaman Barat, Sumatera Barat telah dilakukan pada bulan Oktober 2003 sampai dengan Juni 2004. Penelitian ini bertujuan untuk mendapatkan pseudomonad fluoresen yang berpotensi untuk mengendalikan penyakit layu bakteri, dan meningkatkan pertumbuhan dan produksi nilam. Isolat pseudomonad fluoresen Pf 63, Pf 90, Pf 91, Pf 147, dan Pf 180 sebagai perlakuan diisolasi dari rizosfer nilam sehat, dan diseleksi berdasarkan kemampuan antagonistik terhadap R. solanacearum secara in vitro di Laboratorium Bakteriologi Tumbuhan Fakultas Pertanian UGM. Isolat pseudomonad fluoresen tersebut diintroduksikan ke nilam dan diadaptasikan selama 1 minggu sebelum ditanam. Tanaman yang telah diperlakukan dengan isolat pseudomonad fluoresen ditanam pada kebun yang telah terinfeksi dengan patogen pada bulan Oktober 2003. Percobaan disusun dalam rancangan acak kelompok (RAK) dengan 6 ulangan. Parameter pengamatan adalah masa inkubasi, intensitas penyakit, pertumbuhan tanaman dan produksi minyak nilam. Hasil penelitian menunjukkan bahwa isolat pseudomonad fluoresen dapat mengendalikan penyakit layu bakteri dengan perpanjangan masa inkubasi 6-52 hari dan penekanan intensitas penyakit 31,11 – 50,56%. Disamping itu isolat pseudomonad fluoresen dapat mempengaruhi peningkatan pertumbuhan tanaman yaitu tinggi tanaman (6,7 – 26,3 cm), jumlah daun (4,6 – 30,1 daun/tanaman) dan berat kering daun (24,5 – 154,3 g/tanaman), dan produksi minyak nilam terutama jumlah minyak (4,8 – 22,3 ml/tanaman). Hasil percobaan ini menunjukkan bahwa isolat Pf 91 mempunyai kemampuan antagonistik tertinggi dalam mengen- dalikan penyakit layu bakteri di lapangan. Kata kunci : Nilam, Pogostemon cablin Benth, penyakit layu, bakteri, pengendalian hayati, pseudomonad fluoresen ABSTRACT Controlling bacterial wilt disease on patchouli plant with fluorescent pseudomonad The study of controlling bacterial wilt disease on patchouli plant (Ralstonia solanacearum) with fluorescent pseudomonad was carried out in a farmer’s field in Situak Village West Pasaman, West Sumatera from October 2003 to June 2004. The aims of the study were to find out the effectiveness of fluorescent pseudomonad for controlling bacterial wilt disease, increasing plant growth and production. Isolates of fluorescent pseudomonad Pf 63, Pf 90, Pf 91, Pf 147 and Pf 180 as treatments were isolated from the rhizosphere of healthy patchouli plant, and selected based on antagonistic activity on R. solanacearum in vitro at the Laboratory of Plant Bacteriology, Faculty of Agriculture, UGM. The isolates were inoculated on patchouli plant and adapted for one week before planting. The plants treated with fluorescent pseudomonad isolates were planted in the field infected with pathogen on October 2003. The experiment was arranged in a randomized block design (RBD) with six replications. The assessment parameters were incubation period, disease intensity, plant growth and production of patchouli plants. The results showed that fluorescent pseudomonad isolates could control the bacterial wilt disease and delay the incubation period 6-52 days and decrease the disease intensity 31,11–50,56%. In addition fluorescent pseudomonad isolates could affect the increase of plant growth, i.e. plant height ( 6,7 – 26,3 cm ), leaf numbers (4,6 – 30,1 leaves/plant) and dry weight of leaves (24,5 – 154,3 g/plant), and plant production, especially oil content (4,8 – 22,3 ml/plant). The results of the experiment showed that Pf 91 isolate had the highest antagonistic activity on controlling the bacterial wilt disease on field. Key words : Patchouli, Pogostemon cablin Benth, wilt disease, bacterial, biological control, fluorescent pseudomonad

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Trichoderma cyanodichotomus sp. nov., a new soil-inhabiting species with a potential for biological control

Canadian Journal of Microbiology ◽

10.1139/cjm-2018-0224 ◽

2018 ◽

Vol 64 (12) ◽

pp. 1020-1029

Author(s):

Jishun Li ◽

Yuanzheng Wu ◽

Kai Chen ◽

Yilian Wang ◽

Jindong Hu ◽

...

Keyword(s):

Rna Polymerase Ii ◽

Plant Pathogens ◽

Elongation Factor ◽

Antagonistic Activity ◽

Hunan Province ◽

Dual Culture ◽

Trichoderma Species ◽

Biodiversity Survey ◽

Close Relatives

During a biodiversity survey of Trichoderma (Ascomycota, Hypocreales, Hypocreaceae) in coastal and lake wetlands of China, a new species, Trichoderma cyanodichotomus, was isolated from Dongting Lake wetland of Hunan province. The strain TW21990-1 was characterized as having two types of conidia and producing a distinct blue–green pigment on potato dextrose agar and cornmeal dextrose agar. The taxonomic position was analyzed using three molecular markers, internal transcribed spacer rDNA, translation elongation factor 1-alpha, and RNA polymerase II subunit B, revealing less than 95.0% homology with all known Trichoderma species. The combined phylogenetic tree further identified T. cyanodichotomus as an independent subgroup belonging to Section Pachybasium, with no close relatives. In vitro antagonistic activity by dual-culture assay exhibited broad inhibition against various plant pathogens, including Botryosphaeria dothidea, Pythium aphanidermatum, Rhizoctonia solani, and Verticillium dahliae. In addition, TW21990-1 demonstrated moderate hydrolase activity of cellulase, chitinase, β-1,3-glucanase, and protease, which might be involved in mycoparasitism. Greenhouse experiments showed strong biocontrol effects against tomato damping-off incited by P. aphanidermatum, together with increased seedling height and weight gain. The identification of T. cyanodichotomus will provide useful information for sufficient utilization of fungal resources.

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In Vitro efficacy of essential oils on mycelial growth of Magnaporthe griseaca using blast in basmati rice

International Journal of Agricultural Invention ◽

10.46492/ijai/2016.1.2.8 ◽

2016 ◽

Vol 1 (02) ◽

pp. 170-173

Author(s):

Vipin Kumar ◽

Prashant Mishra ◽

Joginder Singh ◽

Raju .

Keyword(s):

Essential Oils ◽

Radial Growth ◽

Mycelial Growth ◽

Basmati Rice ◽

Pyricularia Grisea ◽

Syzygium Aromaticum ◽

Clove Oil ◽

Neem Oil ◽

Poison Food Technique

Management of Pyriculari agrisea by eco-friendly approaches is promising. In this study the essential oils from Coriander sativum (Coriander), Azadirachta indica (Neem) and Syzygium aromaticum (Clove) were tested against Pyricularia grisea in vitro by poison food technique. The pure culture of P. grisea was allowed to grow at 300ppm, 500ppm and 1000ppm concentration of essential oils on PDA medium. Clove oil was found most effective to inhibit mycelial growth completely at 300, 500 and 1000ppm after72 hrs, 96 hrs and 120 hrs. Next effective oil was observed neem oil followed by coriander oil. This indicated that clove oil is most effective against P. oryzae pathogen. Neem and coriander oil is less effective. The results of the current study indicated that each of the three essential oils tested, reduced the radial growth of P. oryzae.

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Trichoderma Biological Control to Protect Sensitive Maize Hybrids Against Late Wilt Disease in the Field

Journal of Fungi ◽

10.3390/jof7040315 ◽

2021 ◽

Vol 7 (4) ◽

pp. 315

Author(s):

Ofir Degani ◽

Shlomit Dor

Keyword(s):

Biological Control ◽

Growth Parameters ◽

Crop Protection ◽

Antagonistic Activity ◽

Trichoderma Spp ◽

Maize Crop ◽

Trichoderma Species ◽

Maize Cultivars ◽

Pathogen Dna

Late wilt, a disease severely affecting maize fields throughout Israel, is characterized by the relatively rapid wilting of maize plants from the tasseling stage to maturity. The disease is caused by the fungus Magnaporthiopsis maydis, a soil and seed-borne pathogen. The pathogen is controlled traditionally through the use of maize cultivars having reduced sensitivity to the disease. Nevertheless, such cultivars may lose their immunity after several years of intensive growth due to the presence of high virulent isolates of M. maydis. Alternative effective and economical chemical treatment to the disease was recently established but is dependent on the use of a dripline assigned for two adjacent rows and exposes the risk of fungicide resistance. In the current work, eight marine and soil isolates of Trichoderma spp., known for high mycoparasitic potential, were tested as biocontrol agents against M. maydis. An in vitro confront plate assay revealed strong antagonistic activity against the pathogen of two T. longibrachiatum isolates and of T. asperelloides. These species produce soluble metabolites that can inhibit or kill the maize pathogen in submerged and solid media culture growth assays. In greenhouse experiments accompanied by real-time PCR tracking of the pathogen, the Trichoderma species or their metabolites managed to improve the seedlings’ wet biomass and reduced the pathogen DNA in the maize roots. A follow-up experiment carried out through a whole growth session, under field conditions, provided important support to the Trichoderma species’ beneficial impact. The direct addition of T. longibrachiatum and even more T. asperelloides to the seeds, with the sowing, resulted in a yield improvement, a significant increase in the growth parameters and crops, to the degree of noninfected plants. These bioprotective treatments also restricted the pathogen DNA in the host tissues (up to 98%) and prevented the disease symptoms. The results encourage more in-depth research to uncover such biological agents’ potential and the methods to implement them in commercial fields. If adequately developed into final products and combined with other control methods, the biological control could play an important role in maize crop protection against Late wilt.

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