GENETICS AND POPULATION PRACTICABILITY OF USING SNP (C. 232Т>А) OF LEPR GENE AS A MARKER FOR FURTHER SELECTION FOR LARGE WHITE AND MYRGOROD PIG BREEDS

2016 ◽  
Vol 52 ◽  
pp. 176-180 ◽  
Author(s):  
N. K. Sarantseva ◽  
V. M. Balatsky ◽  
V. Y. Nor ◽  
Ye. K. Oliinychenko
Keyword(s):  
Selection Pressure ◽  
Leptin Receptor ◽  
Receptor Gene ◽  
Large White ◽  
Weinberg Equilibrium ◽  
Lepr Gene ◽  
Large White Breed ◽  
White Breed ◽  
Hardy Weinberg Equilibrium ◽  
Selection For

Leptin is an important regulator of energy metabolism and reproduction and is mainly synthesized in the adipocytes and then secreted into bloodstream. Leptin receptor is one of regulating components of organism energetic homeostasis. Receptor influences on leptin effects by regulating feed intake, body weight and fat deposition. Leptin receptor gene (LEPR) is located in the sixth chromosome in the region that correlates with content of intramuscular fat, thickness of back fat, growth rate and pig carcass parameters. Due to these correlations, LEPR is known to be gene candidate that controls quantitative traits. Leptin receptor gene consists of 20 exons; not less than 25 single nucleotide polymorphisms (SNPs) were found in gene structure in different gene sites (exons, introns, 5’ and 3’ regions). SNPs of LEPR gene can be chosen as useful markers for predicting breeding value in pigs. For the experiment SNP c.232T>A was chosen; it is located in the second exon of LEPR gene. The aim of work was to study spreading of SNP c.232Т>А in LEPR gene of breeds under Ukrainian selection; to estimate if marker selection for proving meat quality is possible using chosen SNP as a marker. Materials and methods. For genetic population analysis, DNA samples of Large White breed (bred in Stepne farm, Poltava region, Ukraine) and Mirgorod breed (bred in Dekabristy farm, Poltava region, Ukraine) were used; 50 samples of each breed were taken for the research. Samples were genotyped using PCR-RFLP method. Deviations from genetic equilibrium found using the Hardy-Weinberg coefficient were signified with chi-square criterium, the frequency of alleles, estimation of gene frequencies, determination of heterozygosity were counted using GenAlex 6.0. Results. Genetic researches showed polymorphism c. 232Т>А in LEPR gene to be spread in population of Large White breed and Mirgorod breed under Ukrainian selection. Polymorphism with AA genotype was shown to be spread the most. In studied Large White population highly probable deviation of the actual distribution of genotypes of the expected value for the Hardy-Weinberg equilibrium (χ2 = 15.759, p ≤ 0.001) was found. The deviation was caused by increasing homozygotes (АА = 0.680). Small amount of heterozygotes (АТ = 0.160) and alternative homozygotes (ТТ = 0.160) was found. Positive designation of Rayt index (0.561) and the advantage of expected heterozygosis (0.365) on the actual (0.160) also show existence of selection pressure of LEPR in this herd. In Myrgorod pig population big amount of animals turned out to be homozygotes АА (0,720), small amount of heterozygotes was found (АТ=0.280), alternative homozygotes TT were not found. Deviation from spreading of genotypes of the expected value for the Hardy-Weinberg equilibrium was not significant and did not have a significant nature (χ2 = 1.325); SNP variety (c. 232Т>А) in LEPR gene is not spread, so this SNP in Mirgorod breed wasn’t under selection pressure. The fact of low selection pressure of (c. 232Т>А) in LEPR gene in Mіrgord breed can also be proved of negative designation of Rayt index (-0,163) and domination of heterozygotes (0.280). Allele A is found to be dominative above allele T in both studied populations. Conclusions. After DNA analysis of two breeds under Ukrainian selection (Mirgorod and Large White breeds) polymorphism c. 232Т>А in LEPR gene SNP was found to be spread; chosen SNP can be used for further researches in association analysis for finding correlation between SNP and meat traits.

Association between leptin receptor gene polymorphism and quality of both meat and back fat in large white pigs of ukrainian breeding

2016 ◽  
Vol 3 (2) ◽  
pp. 42-48
Author(s):  
V. Balatsky ◽  
I. Bankovska ◽  
A. Saienko
Keyword(s):  
Gene Polymorphism ◽  
Leptin Receptor ◽  
Receptor Gene ◽  
Intramuscular Fat ◽  
Large White ◽  
Pig Breeds ◽  
Lepr Gene ◽  
Large White Breed ◽  
White Breed

Leptin receptor is one of the components of the system of regulating energy homeostasis of the organism. Leptin receptor gene (LEPR) polymorphism is associated with pig carcass index of the content of intramus- cular fat in its valuable parts, which is particularly important when assessing the quality of their carcasses for processing. Intramuscular fat is associated with meat fl avor characteristics and partly determines its tenderness, juiciness, and other parameters. Aim. To analyze LEPR gene (SNP NM001024587.1, p. 1987 C > T) polymor- phism in populations of various pig breeds and to establish its relationship with the quality of both meat and fat of pigs of Large White breed of Ukrainian breeding. Methods. Genetic-population analysis of nine pig breeds, associative analysis on the search connection of LEPR gene polymorphism with quality of both meat and fat of pigs of Large White breed of Ukrainian breeding. LEPR locus genotyping was performed by High Resolution Melting (HRM). Results. All the studied breeds are characterized by polymorphism of the leptin receptor gene (SNP NM001024587.1, p. 1987 C > T), signifi cant breed specifi city in the distribution of frequencies of alleles was established. Statistically confi rmed effect (p < 0.05) of genotypes LEPR on the content of intramuscular fat, total dry matter and moisture in the meat, as well as the moisture content in the back fat of pigs of Ukrainian Large White breed was revealed. Higher content of intramuscular fat was found in the animals with genotype TT, while a smaller amount of intramuscular fat and more moisture in fat was revealed in heterozygotes. Conclusions. Genetic marker LEPR SNP NM001024587.1, p. 1987 C > T can be used in the marker-assisted selection to predict and improve the performance quality of the meat of pigs of Large White breed of the Ukrainian breeding. These results suggest that porcine leptin receptor gene controls the quality of fat comp- lex – inside muscles and in the dorsal part of the carcass.

Indexes of reproductive ability and their repeatability in sows of different genotypes, taking into account the polymorphism g.1426g> a of the Mc4r gene

2020 ◽  
pp. 49-62 ◽  
Author(s):  
Viktor Khalak ◽  
Vasyl Voloshchuk ◽  
Konstiantyn Pochernyaev ◽  
Serhii Smyslov ◽  
Maria Ilchenko
Keyword(s):  
Receptor Gene ◽  
Live Weight ◽  
Multiple Births ◽  
Large White ◽  
Mc4r Gene ◽  
Litter Weight ◽  
Reproductive Ability ◽  
Large White Breed ◽  
White Breed ◽  
Consolidation Coefficient

The results of studies of the reproductive ability of sows of different genotypes are given taking into account the polymorphism g.1426G> A of the MC4R gene, the factor of repeatability of characters is determined, and the economic efficiency of the research results is calculated. The experimental part of the research was carried out in the conditions of the «Druzhba-Kaznacheevka» LTD in Dnipropetrovsk region. The objects of research were sows of the Large White breed. It was determined that sows of the main herd, according to signs of reproductive ability, belong to class I and class elite. The experiment showed that sows of genotype AG exceeded their peers of others (GG and AA) in terms of “born piglets total, heads” by 1.6 and 0.7 heads, “Live piglets born (multiple births), heads - by 1 6 and 0.9 heads, “the mass of the litter at the time of birth, kg” - by 2.7 - 1.3 kg., “the mass of the litter at the time of weaning at the age of 28-35 days, kg” - by 5, 1 - 3.9 kg. The difference between animals of the indicated genotypes is: for large-foetus - 0.06-0.01 kg, the index "evenness of the sow’s litter in live weight of piglets at birth, points" - 1.05-1.84 points, index of N. D. Berezovsky - 3.75 -2.36 points. The index of the safety of piglets before weaning ranged from 84.4 to 93.0 %. It was proved that the minimum value of the index “evenness of the sow’s littar in live weight of piglets at the time of birth, points (7.76 ± 1,082 points) are characterized by sows of genotype AA, in which the rate of multiple pregnancy ranged from 8 to 14 pigs, and the value of the sign“ large-foetus, kg "equaled 1.37 kg. The repeatability coefficient for sows of the main herd and sows of the Large White breed of different genotypes for the melanocortin 4 (MC4R) receptor gene ranges from -0.916 ± 0.1418 (І-IV farrow, genotype - GG, sign - “piglet safety before weaning, %) to 0.978 ± 0.0738 (I-V farrow, genotype - GG, sign - “large foetus”, kg ”). The maximum value of the phenotypic consolidation coefficient was found in sows of genotype AA according to the following quantitative characteristics: “live piglets (multiple births), heads.”, “litter weight at the time of birth, kg”, “litter weight at weaning age of 28-35 days, kg "," the safety of piglets before weaning ,% ". The sows of the genotype GG belong to the unconsolidated group according to the signs of reproductive qualities. The use of sows of the genotype for the melanocortin 4 (MC4R) AG receptor gene provides additional products at the level of 5.15 %, and its cost is 165.41 UAH /head. Keywords: sow, breed, reproductive ability, MC4R gene, genotypic consolidation coefficient, variability, correlation, repeatability.

scholarly journals Polygenic hereditary traits of young pigs and their association with the melanocortin receptor gene – 4 (MC4R)

2020 ◽  
Vol 22 (93) ◽  
pp. 84-89
Author(s):  
V. I. Khalak ◽  
B. V. Gutyj
Keyword(s):  
Receptor Gene ◽  
Live Weight ◽  
Melanocortin Receptor ◽  
Thoracic Vertebrae ◽  
Large White ◽  
Large White Breed ◽  
Young Pigs ◽  
Melanocortin 4 Receptor Gene ◽  
White Breed ◽  
Fat Thickness

The article presents the results of studies of fattening and meat qualities of young pigs of different genealogical lines of large white breed and genotypes by the gene of melanocortin receptor – 4 (MC4R). The research was conducted in “Druzhba-Kaznacheyivka” LLC, the genetics laboratory of the Institute of Pig Breeding and APV NAAS and the animal husbandry laboratory of the State Institution Institute of Grain Crops NAAS. The work was performed according to the research program of NAAS №30 “Innovative technologies of breeding, industrial and organic production of pig products” (“Pig Breeding”). DNA-typing of the melanocortin-4 receptor gene (MC4R) was performed by Kim K., Larsen N., Short T. et al. (2000). Evaluation of young pigs for fattening and meat qualities was carried out taking into account the following quantitative characteristics: the average daily increase in live weight during the period of control fattening, g; age of live weight 100 kg, days; fat thickness at the level of 6–7 thoracic vertebrae, mm; the length of the cooled carcass cm; the length of the bacon half of the cooled half-carcass, cm. The length of the cooled carcass was measured with a measuring tape from the edge of the fusion of the pubic bones to the anterior surface of the first cervical vertebra; the length of the bacon half of the cooled half-carcass - from the anterior edge of the pubic bone to the middle of the anterior edge of the first rib (Berezovsky, Khatko, 2005). Conditions for feeding and keeping young pigs of the experimental groups were identical and complied with zootechnical standards. Economic efficiency of use of animals of various genotypes (МС4RАА, МС4RАG) and biometric processing of the received data (Lakin, 1990). calculated according to generally accepted methods. It is established that young pigs of large white breed of controlled population at the age of reaching live weight of 100 kg exceed the minimum requirements of the elite class by 6.73, fat thickness at the level of 6–7 thoracic vertebrae – 30.84, length of chilled carcass – 3.52 %. Significant difference between animals of different genotypes by melanocortin 4 receptor gene (МС4RАА, МС4RАG) was established by the average daily gain of live weight during the control period of fattening, the age of reaching live weight of 100 kg, fat thickness at the level of 6–7 thoracic vertebrae, length half of the cooled half-carcass and a complex index of fattening and meat qualities (B. Tyler's index). Young pigs of large white breed line Kotilo UA 8819347 outperformed peers line Azuro UA 8800557 on average daily live weight gain during the control period of fattening by 5.29%, the age of 100 kg live weight – 3.29 %, fat thickness at the level of 6–7 breasts vertebrae – 7.14 %. The longer length of chilled carcass (by 1.13 %) and the length of bacon half of chilled half carcass (by 2.68 %) were characterized by animals of Azuro UA 8800557. The correlation coefficient between fattening and meat qualities in young pigs of large white breed of the controlled herd varies ranging from –0.865 (tr = 22.26, P < 0.001) to +0.913 (tr = 35.43, P < 0.001). The maximum increase in additional products was obtained from young pigs of the МС4RАG genotype (+2.57 %) and the genealogical line Kotilo UA 8819347 (+4.86 %), and its cost is +694.26 – 1312.88 UAH/head. in accordance.

scholarly journals Fattening and meat qualities of store pigs of large white breed of different intra-breed differentiation by melanocortin-4 receptor gene (MC4R)

2020 ◽  
Vol 23 (9) ◽  
pp. 30-37
Author(s):  
Viktor Khalak
Keyword(s):  
Weight Gain ◽  
Receptor Gene ◽  
Live Weight ◽  
Thoracic Vertebrae ◽  
Large White ◽  
Large White Breed ◽  
Melanocortin 4 Receptor ◽  
Melanocortin 4 Receptor Gene ◽  
White Breed ◽  
Fat Thickness

The study presents the results of research on fattening and meat qualities of store pigs of large white breed of different intra-breeding differentiation by the gene of melanocortin-4 receptor (MC4R). The study calculated the economic efficiency of animals of different genotypes. The following fattening and meat qualities were evaluated: average daily increase in live weight during the period of control fattening, g; age of live weight 100 kg, days; fat thickness at the level of 6-7 thoracic vertebrae, mm; the length of the cooled carcass, cm; length of bacon half of chilled half-carcass, cm. It was established that store pigs of controlled population at the age of reaching live weight of 100 kg, fat thickness at the level of 6-7 thoracic vertebrae, and length of chilled carcass exceed the minimum requirements of the elite class by 13.69%. A significant difference between animals of different genotypes by the gene of the melanocortin-4 receptor (MC4RAA, MC4RAG) was established according to the average daily live weight gain during the control fattening period (4.24%, P <0.01), the age of reaching a live weight of 100 kg (2.18%, P <0,01), fat thickness at the level of 6–7 thoracic vertebrae (8,45%, P <0,05), length of chilled carcass (2,26%, P <0,001), length of bacon half of chilled half carcass (3.36%, P <0.001) and selection index (SI) (18.92%, P <0.05). Significant correlations in animals of different genotypes by the melanocortin-4 receptor gene (MC4RAA, MC4RAG) were identified regarding the following features: average daily live weight gain during the control period of fattening × age of 100 kg live weight (-0.475 – -0.853), average daily live weight for the period of control fattening × SI (+0.686 - +0.770), SI × age of reaching live weight 100 kg (+0.515 – -0.721), SI × fat thickness at the level of 6-7 thoracic vertebrae (-0.944 – -0.885), length of chilled carcass × length of bacon half of chilled carcass (+0.899 – +0.861). The maximum increase in additional products was obtained from the sale of young pigs by the gene for the melanocortin-4 receptor MC4RAG – +2.02%.

scholarly journals Hybridization as a method of improving quality of pork

2020 ◽  
pp. 31-35
Author(s):  
B. P. Kovalenko ◽  
◽  
O. B. Shevchenko ◽  
Keyword(s):  
Chemical Composition ◽  
Physical Properties ◽  
Ph Value ◽  
Longissimus Dorsi ◽  
Large White ◽  
Group Iii ◽  
Large White Breed ◽  
White Breed ◽  
A Share

Studies were carried out on the main indicators of the chemical composition and physical properties of muskulus longissimus dorsi of pigs of different genotypes during hybridization. The indicators of the chemical composition and physical properties of muskulus longissimus dorsi were determined by conventional methods. The materials of experimental studies were processed using methods of variation statistics. According to the amount of moisture, no significant difference between the control and experimental groups was established. According to the amount of protein in meat, a tendency to a slight decrease, compared with the control group by 1.4 (group III) … 1.9 % (group II) was established. The use of boars of Poltava meat breed led to a decrease in intramuscular fat in descendants by 0.25 % compared with peers of a large white breed (P <0.95), and precocious meat - to a real increase of this indicator by 0.43 % at P> 0 , 95. A tendency towards an increase in the amount of tryptophan (by 0.04 ... 0.05) and oxyproline (by 0.004 ... 0.005) in the muscles of hybrid animals was revealed in comparison with peers of pure breed of large white breed. In terms of the biological usefulness of meat proteins, animals of group III were the best - in terms of the quality of the protein of the longest muscle of the back, their advantage over peers of group II was 0.07, and compared with purebred peers. Group I - 0.12 (p <0.95). It was found that with the same area of the meat spot both in purebred large white breeds of animals and hydrides of different genotypes, there is a tendency to increase the latter compared to the control in a humid place: with a «share» of «blood» ½ pm by 2.2 %, and with a «share» of blood ½ cm - 1.4 %. A tendency has been established to increase the pH value of the longest back muscle in animals with a «share» of blood of specialized meat breeds compared with a large white breed. In hybrid animals with a «share» of blood of Poltava meat and precocious meat breeds, there is a tendency to increase the pH value in comparison with the large white breed by 0.15 (2.8 %) and by 0.17 (3.1 %), i.e. the meat of hybrid pigs, the pH of which has a shift towards alkalinity due to less accumulation of lactic acid, has a worse preservation.

scholarly journals ЗВ'ЯЗОК G.143C>T ПОЛІМОРФІЗМУ ГЕНА CTSL ІЗ ВІДГОДІВЕЛЬНИМИ І М’ЯСНИМИ ЯКОСТЯМИ СВИНЕЙ ВЕЛИКОЇ БІЛОЇ ПОРОДИ УКРАЇНИ

2014 ◽  
pp. 88-90
Author(s):  
К. Ф. Почерняєв ◽  
В. М. Балацький ◽  
І. К. Лядський
Keyword(s):  
Impact Force ◽  
Large White ◽  
Meat Yield ◽  
Large White Breed ◽  
White Breed ◽  
Different Populations ◽  
The Impact

Викладені результати досліджень зв’язку гена ctslіз відгодівельними і м’ясними якостями свиней вели-кої білої породи України. Проаналізовано особливостіалелофонду гена ctsl у різних популяціях свиней ВБпороди; проведено аналіз частот і розповсюдженнярізних генотипів; вивчено зв'язок g.143C>T полімор-фізму цього гена з товщиною відкладення сала посередині спини та в ділянці 6–7-го ребер, а також ізсередньодобовими приростами,товщиною найдовшо-го м’яза спини й виходом м’яса. Визначено силу впли-ву g.143C>T поліморфізму гена ctsl на ці ознаки.Встановлено достовірні відмінності (P<0,05) за де-якими з вищезгаданих ознак у свиней із різними гено-типами гена ctsl. На підставі проведених дослідженьзроблені висновки та подані пропозиції виробництву. The results of researches of the association of gene ctsl   with the formation of fattening and meat qualities in pigs of the Large White breed of Ukraine are given. It has been analysed the features of gene ctsl alelofund in different populations of pigs of LW breed, it was carried out the analysis of frequencies and distribution of different genotypes. The association g.143C>T polymorphism of this gene with thick deposits of fat in the middle of the back and in area of 6-7 ribs, as well as average daily gains , thickness of the longest muscles and meat yield was studied. It has been determined the impact force g.143C>T polymorphism of gene ctsl on these traits. It was found out significant differences (P <0.05) for some of the above traits in pigs with different genotypes of gene ctsl. On the basis of the researches it was made conclusions and advanced proposals to producers.

scholarly journals EFFICIENCY OF INTRAUTERINE INSEMINATION IN BREEDING SOWS

2017 ◽  
Vol 53 ◽  
pp. 254-259
Author(s):  
V. O. Melnik ◽  
O. O. Kravchenko ◽  
О. S. Kohut
Keyword(s):  
Artificial Insemination ◽  
Intrauterine Insemination ◽  
Live Weight ◽  
Reproductive Technologies ◽  
Breeding Value ◽  
Large White ◽  
Large White Breed ◽  
Significant Difference ◽  
White Breed ◽  
Breeding Farms

Improving of fertility indicators and reproductive qualities of sows during artificial insemination in farms of different specializations is very topical issue. The introduction of artificial insemination of sows on breeding farms by the spermdoses of optimum volume, by the frozen-thawed and sex sperm requires the introduction of innovative reproductive technologies. The use of economical methods of artificial insemination of sows using a minimum number of sperm in a small volume of spermdose in order to achieve high rates of fertility and prolificacy was proven in numerous experiments of the authors. Significantly reduced spermdose may be sufficient if the sperm enters deep enough into the uterus. Vitality of sperm does not depend on the size of spermdose, but the best place for sperm to survive one oviducts where they keep the fertilizing capacity from 9 to 27 hours. So deep intrauterine insemination of sows improves conditions for sperm survival The aim was to study the feasibility and justification for widespread implementation in to production on breeding farms of intrauterine insemination of sows morder to increase their fertilization and prolificacy and  to save the boar sperm with the highest index of breeding values. Experiments were conducted in terms of selection and genetic center of Agrofirm "Mig-Service-Agro" in Mykolaiv region. In the experiment used 65 sows of live weight of 280-320 kg wiht 2-4 farrowing were. Sows in sexual hunting were showed once daily in the morning using a boar-prober. Artificial insemination was performed twice: the first time - in the afternoon and at 14-16 p.m. The second time – in the morning of the next day at 9-10 am. For artificial insemination of sows were ed using experimental spermodes with volume of 40 ml which contained 1.5 billion of a Active sperm. For the dilution of sperm was used Durasperm - KRUUSE (Denmark) the period of sperm perpetuation is 5-7 days. To enter the were semen used catheters Magaplus S, (Spain) for intrauterine insemination of sows. Analysis shows that the period from weaning to insemination has significant difference comparing sows of large White breed with Landrace breed (p <0.001), with genotype sows F1 (p <0.01) and sows of the Duroc breed (p <0.05). For all selected 65 sows duration of suckling period, was estimated which averaged 32.2 days and the average time from weaning of pigs to their sexual inclination and the first intrauterine insemination 6.8 days that meets the physiological norm. After intrauterine insemination of sows of then 48 farrowed, which wich made for 73.9%. іncluding live 5 emergency farrow  were obtained, representing 10.4% of all amount. Percentage of farrow is considered physiologically normal – 80%, or more of total insemined sows. The very low percentage of farrow 53.3% had of sows F1, and the highest percentage was found by sows of the Duroc breed – 85.7%. Pregnency of sows were received just 17, which made 26.1% and highest percentage – 46.7% was set by sows F1. Analysis of the pregnancy sows shows that on average it is 116.2 days was the longest – 117.1 days was set in Landrace breed sows and the short est 115.5 days in Large White breed, but the difference is not significant. 571 pigs were received, іncluding live 451 head, which is 78.9%. The largest percentage of іncluding live piglets obtained from sows F1 – 82.5%, and the lowest in Landrace breeds – 77.0% and Large White – 77.3%. Exit of all piglets per sow without emergency farrowings is 12.2, іncluding live – 9,8. The highest yield were obtained piglets from sows F1 – 13.1, іncluding live – 10.9, the lowest yield of sows of the Duroc breed – 10.6, іncluding live 9.1, which has significant difference compared with the control (IDPs) and other breeds.  After intrauterine insemination 8 sows showed cyclic deregulation in 20-25 days, ie repeated sexual hunt took place on average 22.3 days. These sows were inseminated by not fractional way, they farrowed and an average litter just 13.1 piglets per sow, іncluding live - 11.3 was obtained. Repeating after intrauterine insemination on 45-48-49 day in the sexual hunt came about three sows for artificial insemination by not fractional method 3 farrowed and was obtained output – 13.3 piglets, іncluding live – 11.7. It should be noted that the best sow Large White breed №12 after intrauterine insemination bore 16 pigs, іncluding live 11, sow of Landrace breed №1556 – 18 pigs, іncluding live 12, Duroc №5888 – 13 pigs, іncluding live 11, sow F1 №167 – 20 pigs, іncluding live 14 pigs. Breeding requires more careful handling with major sows taking into account their breeding value and cost, that’s why we believe that there is no need to risk causing injury genitals with intrauterine insemination if a sufficient number of spermdoses of boars-sires exist.

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