scholarly journals Polygenic hereditary traits of young pigs and their association with the melanocortin receptor gene – 4 (MC4R)

Author(s):  
V. I. Khalak ◽  
B. V. Gutyj

The article presents the results of studies of fattening and meat qualities of young pigs of different genealogical lines of large white breed and genotypes by the gene of melanocortin receptor – 4 (MC4R). The research was conducted in “Druzhba-Kaznacheyivka” LLC, the genetics laboratory of the Institute of Pig Breeding and APV NAAS and the animal husbandry laboratory of the State Institution Institute of Grain Crops NAAS. The work was performed according to the research program of NAAS №30 “Innovative technologies of breeding, industrial and organic production of pig products” (“Pig Breeding”). DNA-typing of the melanocortin-4 receptor gene (MC4R) was performed by Kim K., Larsen N., Short T. et al. (2000). Evaluation of young pigs for fattening and meat qualities was carried out taking into account the following quantitative characteristics: the average daily increase in live weight during the period of control fattening, g; age of live weight 100 kg, days; fat thickness at the level of 6–7 thoracic vertebrae, mm; the length of the cooled carcass cm; the length of the bacon half of the cooled half-carcass, cm. The length of the cooled carcass was measured with a measuring tape from the edge of the fusion of the pubic bones to the anterior surface of the first cervical vertebra; the length of the bacon half of the cooled half-carcass - from the anterior edge of the pubic bone to the middle of the anterior edge of the first rib (Berezovsky, Khatko, 2005). Conditions for feeding and keeping young pigs of the experimental groups were identical and complied with zootechnical standards. Economic efficiency of use of animals of various genotypes (МС4RАА, МС4RАG) and biometric processing of the received data (Lakin, 1990). calculated according to generally accepted methods. It is established that young pigs of large white breed of controlled population at the age of reaching live weight of 100 kg exceed the minimum requirements of the elite class by 6.73, fat thickness at the level of 6–7 thoracic vertebrae – 30.84, length of chilled carcass – 3.52 %. Significant difference between animals of different genotypes by melanocortin 4 receptor gene (МС4RАА, МС4RАG) was established by the average daily gain of live weight during the control period of fattening, the age of reaching live weight of 100 kg, fat thickness at the level of 6–7 thoracic vertebrae, length half of the cooled half-carcass and a complex index of fattening and meat qualities (B. Tyler's index). Young pigs of large white breed line Kotilo UA 8819347 outperformed peers line Azuro UA 8800557 on average daily live weight gain during the control period of fattening by 5.29%, the age of 100 kg live weight – 3.29 %, fat thickness at the level of 6–7 breasts vertebrae – 7.14 %. The longer length of chilled carcass (by 1.13 %) and the length of bacon half of chilled half carcass (by 2.68 %) were characterized by animals of Azuro UA 8800557. The correlation coefficient between fattening and meat qualities in young pigs of large white breed of the controlled herd varies ranging from –0.865 (tr = 22.26, P < 0.001) to +0.913 (tr = 35.43, P < 0.001). The maximum increase in additional products was obtained from young pigs of the МС4RАG genotype (+2.57 %) and the genealogical line Kotilo UA 8819347 (+4.86 %), and its cost is +694.26 – 1312.88 UAH/head. in accordance.


2020 ◽  
Vol 23 (9) ◽  
pp. 30-37
Author(s):  
Viktor Khalak

The study presents the results of research on fattening and meat qualities of store pigs of large white breed of different intra-breeding differentiation by the gene of melanocortin-4 receptor (MC4R). The study calculated the economic efficiency of animals of different genotypes. The following fattening and meat qualities were evaluated: average daily increase in live weight during the period of control fattening, g; age of live weight 100 kg, days; fat thickness at the level of 6-7 thoracic vertebrae, mm; the length of the cooled carcass, cm; length of bacon half of chilled half-carcass, cm. It was established that store pigs of controlled population at the age of reaching live weight of 100 kg, fat thickness at the level of 6-7 thoracic vertebrae, and length of chilled carcass exceed the minimum requirements of the elite class by 13.69%. A significant difference between animals of different genotypes by the gene of the melanocortin-4 receptor (MC4RAA, MC4RAG) was established according to the average daily live weight gain during the control fattening period (4.24%, P <0.01), the age of reaching a live weight of 100 kg (2.18%, P <0,01), fat thickness at the level of 6–7 thoracic vertebrae (8,45%, P <0,05), length of chilled carcass (2,26%, P <0,001), length of bacon half of chilled half carcass (3.36%, P <0.001) and selection index (SI) (18.92%, P <0.05). Significant correlations in animals of different genotypes by the melanocortin-4 receptor gene (MC4RAA, MC4RAG) were identified regarding the following features: average daily live weight gain during the control period of fattening × age of 100 kg live weight (-0.475 – -0.853), average daily live weight for the period of control fattening × SI (+0.686 - +0.770), SI × age of reaching live weight 100 kg (+0.515 – -0.721), SI × fat thickness at the level of 6-7 thoracic vertebrae (-0.944 – -0.885), length of chilled carcass × length of bacon half of chilled carcass (+0.899 – +0.861). The maximum increase in additional products was obtained from the sale of young pigs by the gene for the melanocortin-4 receptor MC4RAG – +2.02%.



Author(s):  
Віктор Халак

The results of studies of fattening and meat qualities of young pigs of large white breed, some biochemical parameters of blood serum (urea content, aspartate aminotransferase (AsAT) activity, alanine aminotransferase (AlAT) activity are presented), as well as the economic efficiency of research results is calculated. The study was conducted in LLC "Druzhba-Kaznacheyivka"of Dnipropetrovsk region, in the Research center of biosafety and ecological control of agricultural resources of Dnipropetrovsk state agrarian and economic university, meat processing plant «Jazz» and Laborator Livestock y of the State institution Institute of grain crops of NAAS of Ukraine. The work was performed according to the research program of NAAS of Ukraine №30 "Innovative technologies of breeding, industrial and organic production of pig products" ("Pig breeding"), state registration number 0116U001247. The object of the study was young pigs of large white breed. Evaluation of animals for fattening and meat qualities was carried out taking into account the following indicators: average daily live weight gain during the control period of fattening, g, age of live weight 100 kg, days, fat thickness at the level of 6-7 thoracic vertebrae, mm, length of chilled carcass , cm, the length of the bacon half of the cooled half-carcass, cm (M.D. Berezovsky, I.V. Kha’tko, 2005). A comprehensive assessment of young pigs for fattening and meat qualities was calculated according to the B. Tailer index (P.A. Vashchenko, 2019), biometric indicators - according to the methods of G.F. Lakin (1990). Economic efficiency of research results was calculated according to the generally accepted technique (Methodology for determining the economic ..., 1983). It was found that the urea content, activity of aspartate aminotransferase (AsAT) and alanine aminotransferase (AlAT) in the serum of young pigs of the experimental group corresponds to the physiological norm of clinically healthy animals and amount to 4.77±0.576 mol/l, 68.11±6.445 units/l, and 4.22±3.209 units/l, respectively. According to the age of reaching a live weight of 100 kg (days), the thickness of the fat at the level of 6-7 thoracic vertebrae (mm) and the length of the chilled carcass (cm) of the specified production group and breed correspond to class I and class "elite". The maximum values of "average daily gain of live weight during the period of control fattening, kg", "length of chilled carcass, cm", "length of bacon half of chilled carcass, cm" and minimum values of "age of live weight 100 kg, days" and "fat thickness per levels of 6-7 thoracic vertebrae, mm "are characterized by animals in which the complex index of fattening and meat qualities (B. Tailer's index) ranges from 157.68 to 182.36 points. The number of reliable relationships between the biochemical parameters of blood serum, fattening and meat qualities of young white pigs is 26.67 %. The cost of additional products obtained from young pigs with B. Tailer index of 157.68 – 182.36 points is equal to +1732.04 UAH/head.



Author(s):  
V. I. Khalak ◽  
B. V. Gutyj ◽  
O. I. Stadnits’ka

The results of studies of fattening and meat qualities of young pigs of different origin and intensity of formation in early ontogenesis are presented.  The economic efficiency of the results of the studies is calculated. The study was conducted under the conditions of pedigree reproducer for breeding pigs of the large white breed of LTD “Druzhba-Kaznacheevka” of Dnipropetrovsk region. The object of study was young pigs of English and Hungarian origin, as well animals of varying intensity of formation in early ontogenesis. Estimation of young pigs by absolute and integrated indicators of fattening and meat qualities was carried out taking into account the following quantitative characteristics: average daily weight gain for the period of control fattening, kg, age of reaching live weight of 100 kg, days, thickness of the lard at the level of 6–7 thorax , mm, length of cooled carcass, cm, length of bacon half carcass, cm, index “intensity of formation” (∆t), selection index (SI) and “T-factor”. The economic efficiency of the results of the research was calculated according to the indicator "average daily increase of live weight during the period of control fattening, kg". The analysis of the results of the research shows that the young pigs of the large white breed of the control herd by the age of reaching the live weight of 100 kg were outweighed by the minimum requirements of the Instruction for pig testing by 9.78%, by the thickness of the lard at the level of 6–7 thoracic vertebrae – by 30.33% and by length of the cooled carcass – by 3.52%. Taking into account the class of origin and index “intensity of formation, points” (∆t), the difference between the groups by age of reaching live weight of 100 kg, the thickness of the pelvis at the level of 6–7 thoracic vertebrae and the length of the cooled carcass ranges from 2.24 to 8.03%. It was found that in terms of the main indicators of fattening and meat quality, the young pigs of the large white breed of Hungarian breeding are more consolidated (К1 = 0.085–0.344, К2 = 0.086–0.338). The maximum increment of additional production (average daily weight gain during the control fattening period, kg) was obtained from young pigs of large white breed of English origin (2.39%) and animals with index ∆t at the level of 0.619–0.748 points (1.38%). To evaluate the animals of the main herd (brood boars, sows) according to the fattening and meat characteristics of their offspring, we suggest the use of SI and T-factor breeding indices.



Author(s):  
Viktor Khalak ◽  
Vasyl Voloshchuk ◽  
Konstiantyn Pochernyaev ◽  
Serhii Smyslov ◽  
Maria Ilchenko

The results of studies of the reproductive ability of sows of different genotypes are given taking into account the polymorphism g.1426G> A of the MC4R gene, the factor of repeatability of characters is determined, and the economic efficiency of the research results is calculated. The experimental part of the research was carried out in the conditions of the «Druzhba-Kaznacheevka» LTD in Dnipropetrovsk region. The objects of research were sows of the Large White breed. It was determined that sows of the main herd, according to signs of reproductive ability, belong to class I and class elite. The experiment showed that sows of genotype AG exceeded their peers of others (GG and AA) in terms of “born piglets total, heads” by 1.6 and 0.7 heads, “Live piglets born (multiple births), heads - by 1 6 and 0.9 heads, “the mass of the litter at the time of birth, kg” - by 2.7 - 1.3 kg., “the mass of the litter at the time of weaning at the age of 28-35 days, kg” - by 5, 1 - 3.9 kg. The difference between animals of the indicated genotypes is: for large-foetus - 0.06-0.01 kg, the index "evenness of the sow’s litter in live weight of piglets at birth, points" - 1.05-1.84 points, index of N. D. Berezovsky - 3.75 -2.36 points. The index of the safety of piglets before weaning ranged from 84.4 to 93.0 %. It was proved that the minimum value of the index “evenness of the sow’s littar in live weight of piglets at the time of birth, points (7.76 ± 1,082 points) are characterized by sows of genotype AA, in which the rate of multiple pregnancy ranged from 8 to 14 pigs, and the value of the sign“ large-foetus, kg "equaled 1.37 kg. The repeatability coefficient for sows of the main herd and sows of the Large White breed of different genotypes for the melanocortin 4 (MC4R) receptor gene ranges from -0.916 ± 0.1418 (І-IV farrow, genotype - GG, sign - “piglet safety before weaning, %) to 0.978 ± 0.0738 (I-V farrow, genotype - GG, sign - “large foetus”, kg ”). The maximum value of the phenotypic consolidation coefficient was found in sows of genotype AA according to the following quantitative characteristics: “live piglets (multiple births), heads.”, “litter weight at the time of birth, kg”, “litter weight at weaning age of 28-35 days, kg "," the safety of piglets before weaning ,% ". The sows of the genotype GG belong to the unconsolidated group according to the signs of reproductive qualities. The use of sows of the genotype for the melanocortin 4 (MC4R) AG receptor gene provides additional products at the level of 5.15 %, and its cost is 165.41 UAH /head. Keywords: sow, breed, reproductive ability, MC4R gene, genotypic consolidation coefficient, variability, correlation, repeatability.



2020 ◽  
Vol 60 ◽  
pp. 5-11
Author(s):  
I. V. Verbuch ◽  
H. V. Bratkovska

Goal. To accelerate the selection process and create populations of pigs with high level of productivity to assess the most effective modern methods breeding value of large white pigs in the SE "DG" Pasichna "Institute of Feed and Agriculture Podillya NAAS" Starosinyavsky district of Khmelnytsky region on the basis of a combined assessment of repair young for their own productivity and assessment meat qualities by MD Berezovsky and B. Tyler and the most promising method BLUP. Methods. Comparison, zootechnical and biometric analyzes. Results. The breeding value of pigs of large white breed of the breeding farm of SE "Pasichna" ICSGP NAAS "Starosinyavsky district of Khmelnytsky region by modern effective methods, namely: BLUP. According to the average indicators of the combined assessment of own productivity of animals at control fattening, taking into account their live weight on the date of birth and weaning, average daily gain, live weight and age of 100 kg, body length and fat thickness, boars significantly outweighed pigs. Compared with pigs, their live weight at the date of birth and weaning (at the age of 30 days) was higher by 0.1 and 0.81 kg. The age of reaching a live weight of 100 kg exceeded by 5.43 days, the average daily gain of 0.016 kg. Live weight and body length of pigs on the date of fat thickness measurement was greater by 4.04 kg and 1.03 cm. Lifetime fat thickness at three measurement points at the level of 6–7 thoracic vertebrae, on the buttocks and in the midpoint of the back between withers and buttocks was thinner by 0.92; 0.94 and 1.06 mm. Evaluation indices of fattening and meat qualities by growth energy and fat thickness (Ia) and average daily gain and fat thickness (Iv) in boars indicate their higher level by 2.07 and 7.67 points compared to pigs and are equal to, respectively, 95.12 ± 1.95 and 152.96 ± 2.38 points. The coefficient of variability (Cv, %) of indicators of own productivity and estimated indices of fattening and meat qualities of repair boars ranged from 1.92% (body length on the date of fat thickness measurement, cm) to 15.12% (age of live weight 100 kg, days), repair pigs – from 4.63% (body length on the date of fat thickness measurement, cm) to 28.19% (index of assessment for growth energy and fat thickness, (Ia), points). The best average values of indicators of breeding value of animals are found on the basis of modern, most promising method BLUP, because it is considered in terms of mathematics, statistics and selection is the most accurate, well-founded, has the best theoretical basis and the lowest error variance. The average value of the BLUP index (parent lines) for boars of large white breed is 101.71 ± 1.681, pigs – 96.94 ± 0.724 points. At the same time, boars outweighed pigs by 4.77 points. The most positive correlation between live weight of piglets at weaning with their body length (r = 0.16), average daily gain (r = 0.19) and fat thickness (r = 0.24), the average correlation between the indices of fattening and meat qualities according to MD Berezovsky and B. Tyler (r = 0.56) P > 0.99 and the weak correlation between live weight of piglets at weaning and the BLUR index (r = 0,21) P > 0.99. It should be noted that there are almost no correlations between the fattening and meat quality indices of pigs and the BLUP index. Conclusions. In the conditions of breeding and specialized farms for the production of commercial pork in the region, to determine the breeding value of animals for further reproduction of their own herd and sale to other breeding farms of Ukraine, to systematically assess repair young animals for their own productivity and clear qualities of pigs and the most perfect BLUP index.



Author(s):  
В. О. Горобець

У статті викладені результати оцінювання сви-ней іноземного походження та кровності за відгоді-вельними й м’ясними ознаками з визначенням кращихпоєднань батьківських генотипів для одержанняефекту гетерозису у потомків. Дослідженнями вста-новлено, що найвищу інтенсивність росту під часвідгодівлі мав гібридний молодняк, одержаний у разісхрещування двохпородних свиноматок (велика білафранцузького походження х дюрок німецького поход-ження) з кнурами породи п’єтрен англійського поход-ження. Найбільш відчутне зниження у потомків тов-щини шпику на рівні 6–7-го грудних хребців та підви-щення маси задньої третини півтуші забезпечитьвикористання на заключному етапі трьохпородногосхрещування кнурів породи п’єтрен англійського по-ходження. Чистопородне розведення свиней великоїбілої породи не сприяє підвищенню відгодівельних ім’ясних ознак у молодняка, навіть за об’єднання в їхгенотипі спадковості тварин французького, німецько-го й англійського походження. Прибутковість відго-дівлі молодняка свиней в умовах сучасного промисло-вого виробництва повинно ґрунтуватися на поєдну-ваності порід, особливо, коли використовуютьсятварини іноземного походження. The article presents the results of the evaluation of pigs of foreign origin and crownest of  fattening and meat traits with determining of the best combinations of parental genotypes to obtain the effect of heterosis in the descendants. Research has shown that a hybrid  young stock had the highest growth rate during feeding, who was obtained by crossing dohorty sows (large white of French origin x Duroc of German origin) with grunts of Pietrain breed of English origin. The largest declines of the young stock’s backfat thickness at 6-7 thoracic vertebrae and increase the mass of the posterior third of the side will ensure three times Pietrain boars’ cross breeding of English origin at the final stage. Purebred breeding of pigs of large white breed does not promote the fattening and meat traits in young stock, even for the unification of their genotype heredity animals of French, German and English origin. The profitability of fattening of young pigs in the conditions of modern industrial production should be based on associations of species, particularly when animals of foreign origin are used.



Author(s):  
М. С. Небилиця

Наведено перші результати оцінки молодняку сви-ней великої білої породи племінних господарств об-ластіBLUPметодом. Визначено коефіцієнти коре-ляції між показниками різних методів оцінки та основними селекційними ознаками у ході зняття тварин із контрольного вирощування. Встановлено, що показник оцінки молодняку свиней вітчизняної селекції BLUP методом вірогідно корелює з показ-никами індексної, рангової, а також оцінки за неза-лежними рівнями. Однак найвищі коефіцієнти коре-ляції встановлено з оціночними індексами за серед-ньодобовим приростом і товщиною шпику (r = 0,52) та енерґією росту й товщиною шпику (r = 0,40). The first assessment results of young pigs of large white breed of pedigree farms of the region by BLUP method are presented.  The correlation coefficients between indicators of   different methods of assessment and core breeding signs, when   animals are removed from the   control of cultivationare defined.  It is  found that the rate of assessment of pigs by BLUP method is strongly correlated with the indicators  of  the  index,  the  rank  and  evaluation by  independent  levels.  However,    higher correlation coefficients with estimated indices of the daily increase  of live weight of pigs and thickness of pork fat (r = 0,52) and energy growth and thickness of pork fat (r = 0,40) is installed.



Author(s):  
V. V. Bondarenko

The research was conducted on two groups–analogues of young pigs of large white breed with 10 pigs each. Weaned piglets at the age of 28 days were fed with the protein–vitamin mineral supplement «Minaktyvit» – starter in the amount of 25% of grain feed (33 days), while young pigs in growing were given the protein–vitamin mineral supplement «Minaktyvit» – grower in the amount of 15% (50 days) and those in fattening the protein–vitamin mineral supplement «Minaktyvit» – finisher in the amount of 10% (62 days). The control group received protein–vitamin mineral supplement. At the end of growing with the live weight of 100 – 110 kg a control slaughter was carried out and samples of stomach, small and large intestines for laboratory tests were picked out. Studies have shown that feeding of young pigs with the protein–vitamin mineral supplement «Minaktyvit» increases the average daily gains by 95 g or 15.68%, while their level is 701 g in the experimental group of animals and 606 g in the control one. The protein–vitamin mineral supplement «Minaktyvit» incredibly affected the morphological parameters of the stomach in the experimental group of pigs. However, the thickening of the walls by 4.3%, 5% and 10.9% respectively is observed in cardiac, fundic and pyloric areas of the experimental group, compared to the control one. The thickening of the walls in stomach areas under the influence of feed factor is associated with increasing of the mucosa thickness. Mucosa increased by 0.1 mm in the cardiac area, 0.36 mm in fundic and 0.53 mm in the pyloric one. The change in the structure of the investigated jejunum is observed while consuming the protein–vitamin mineral supplement «Minaktyvit» in the diet. The wall thickness increased by 17.57% (P < 0.05), including the thickening of mucosa by 16.7% and that of serous–muscular shell by 18.8%. No significant difference in the structure of the colon in the experimental group of animals was determined.



2021 ◽  
Vol 273 ◽  
pp. 02012
Author(s):  
Olga Tretyakova ◽  
Anna Degtyar ◽  
Alexander Avdeyev ◽  
Dmitry Ovchinnikov ◽  
Irina Morozyuk

The indicators of growth and development, fattening and meat qualities of pigs of the breeding center “Lozovoe” CJSC “Plemzavod-Yubileyny” of the Tyumen region were evaluated. The indicators that characterize the growth and development of young animals were taken into account: live weight, age, average daily growth. When the live weight of 100 kg was reached, an ultrasound device was used to evaluate the thickness of bacon, the depth of muscles and the yield of lean meat, which are in the database of breeding records for 2011-2020. To characterize the meat qualities, 1144 Landrace piglets were slaughtered, 275 - large white breed, 129 - Pietren breed, 339 hybrids (LxKB), 159 hybrids (LxD), 460 hybrids obtained from boars of foreign selection. A comparative analysis of commercial hybrids of various variants of crossing pigs of domestic and foreign selection is carried out. Processing of the research results was carried out in the laboratory of Molecular diagnostics and Biotechnology of the Don State Agrarian University. The influence of the breed is established.



2016 ◽  
Vol 3 (2) ◽  
pp. 42-48
Author(s):  
V. Balatsky ◽  
I. Bankovska ◽  
A. Saienko

Leptin receptor is one of the components of the system of regulating energy homeostasis of the organism. Leptin receptor gene (LEPR) polymorphism is associated with pig carcass index of the content of intramus- cular fat in its valuable parts, which is particularly important when assessing the quality of their carcasses for processing. Intramuscular fat is associated with meat fl avor characteristics and partly determines its tenderness, juiciness, and other parameters. Aim. To analyze LEPR gene (SNP NM001024587.1, p. 1987 C > T) polymor- phism in populations of various pig breeds and to establish its relationship with the quality of both meat and fat of pigs of Large White breed of Ukrainian breeding. Methods. Genetic-population analysis of nine pig breeds, associative analysis on the search connection of LEPR gene polymorphism with quality of both meat and fat of pigs of Large White breed of Ukrainian breeding. LEPR locus genotyping was performed by High Resolution Melting (HRM). Results. All the studied breeds are characterized by polymorphism of the leptin receptor gene (SNP NM001024587.1, p. 1987 C > T), signifi cant breed specifi city in the distribution of frequencies of alleles was established. Statistically confi rmed effect (p < 0.05) of genotypes LEPR on the content of intramuscular fat, total dry matter and moisture in the meat, as well as the moisture content in the back fat of pigs of Ukrainian Large White breed was revealed. Higher content of intramuscular fat was found in the animals with genotype TT, while a smaller amount of intramuscular fat and more moisture in fat was revealed in heterozygotes. Conclusions. Genetic marker LEPR SNP NM001024587.1, p. 1987 C > T can be used in the marker-assisted selection to predict and improve the performance quality of the meat of pigs of Large White breed of the Ukrainian breeding. These results suggest that porcine leptin receptor gene controls the quality of fat comp- lex – inside muscles and in the dorsal part of the carcass.



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