Polymorphisms of genes of DNA repair enzymes and indicators of genome instability in coal mine workers

2020 ◽  
pp. 12-18
Author(s):  
A. V. Ryzhkova ◽  
V. I. Minina ◽  
A. O. Sokolova ◽  
M. L. Bakanova ◽  
R. A. Titov ◽  
...  
Keyword(s):  
Dna Damage ◽  
Dna Repair ◽  
Coal Mining ◽  
Chromosomal Aberrations ◽  
Negative Impact ◽  
Coal Industry ◽  
Genetic Characteristics ◽  
Conflict Of Interests ◽  
Industrial Enterprises ◽  
Polymorphic Variants

Introduction. Coal industry is one of the main economy sectors of many countries. However, it poses a danger to the environment and human health. Since the Kemerovo region is a region with a developed industry, the problem of maintaining genetic homeostasis is highly relevant. Miners are exposed to various harmful factors that can act as genotoxicants and cause various DNA damage.The aim of the study was to explore the associations of polymorphic variants of DNA repair genes with chromosomal instability in coal mining workers.Materials and methods. Polymorphic variants of genes of enzymes of reparation DNA (XPD (rs13181), XPG (rs17655), XRCC2 (rs3218536), XRCC3 (rs861536), XRCC4 (rs2075685), XRCC4 (rs1805377)) and chromosomal aberrations in blood lymphocytes of 307 miners of coal mines in Kuzbass, 338 residents of the Kemerovo region who did not work in industrial enterprises were analyzed.Results. The frequency of chromosomal aberrations in the group of miners (4.01±0.14) was significantly higher than in the group of individuals who did not work in production (1.67±0.06, p<0.0000005). We found that allelic variants in genes XPD (rs13181), XRCC3 (rs861536), XRCC4 (rs2075685), are associated with the increased chromosomal damage in miners.Conclusions. Coal production has a negative impact on the genome of workers and can lead to the formation of cytogenetic disorders, which determines the need to develop measures for the comprehensive prevention of diseases caused by the accumulation of DNA damage. The results of the study will expand the existing understanding of the formation of the human genetic apparatus individual sensitivity to the effects of genotoxic factors, as well as formulate recommendations for coal mining workers in accordance with their genetic characteristics.Conflict of interests. The authors declare no conflict of interests. 

scholarly journals Polymorphism of TGF-β1, TР53, CHEK2, ATM genes аnd chromosomal aberrations in workers at coal thermal electric power station

2021 ◽  
Vol 100 (1) ◽  
pp. 42-48
Author(s):  
Varvara I. Minina ◽  
Yulia A. Nelyubova ◽  
Anastaiya O. Sokolova ◽  
Yana A. Savchenko ◽  
Anastaiya V. Ryzhkova ◽  
...  
Keyword(s):  
Chromosomal Aberrations ◽  
Power Plants ◽  
Thermal Power ◽  
Chromosomal Damage ◽  
Genetic Characteristics ◽  
Industrial Enterprises ◽  
Increased Risk ◽  
Thermal Electric Power Station ◽  
Polymorphic Variants ◽  
Tgf Β1

Introduction. Working conditions at coal-fired power plants cause an increased risk of the formation of chromosomal damage in workers. The contribution of the variability of the structure of the genes controlling the cell cycle and apoptosis to the occurrence of cytogenetic disorders induced by such mutagens has been little studied. Material and methods. The polymorphic variants of the genes: TP53 (rs1042522), CHEK2 (rs555607708), ATM (rs1801516), TGF-β1 (rs1800469), and the level of chromosomal aberrations (CA) in the lymphocytes of the blood were studied. Three hundred twenty-six workers of coal-fired power plants and 267 individuals who did not work in industrial enterprises were examined. All individuals were Caucasians, residents of Kemerovo (Western Siberia, Russia). Results. The level of CA in blood lymphocytes in thermal power plant workers was established to be statistically significantly higher than that of residents of the same locality who never works in industrial enterprises (3.05 ± 0.09% versus 1.67 ± 0.07%, p = 0, 000001). The prevalence of the polymorphic variants of the TP53 (rs1042522), CHEK2 (rs555607708), ATM (rs1801516), TGF-β1 (rs1800469) genes in the group of workers was the same to those in the comparison group and corresponded to the global data obtained in Caucasians. In the group of workers with the TT genotype of the TGF-β1 gene, the frequency of metaphases with chromosome damage was statistically significantly higher than in individuals with the CC genotype (4.07 ± 0.41% versus 2.85 ± 0.10%, p = 0.006). Discussion. For the first time a significant contribution of polymorphic variants of TGF-β1 genes to the formation of chromosomal damage in working coal-fired power plants was revealed. This may be associated with an increased level of expression of the multifunctional cytokine TGF-β1 in owners of the TT genotype and its ability to influence proliferation, apoptosis, telomerase activity, and other critical cellular processes. Conclusion. The results of the study I, ndicate that not only the complex of industrial genotoxicants, but also individual genetic characteristics of the organism can influence on the formation of cytogenetic disorders in workers at thermal power plants.

ENVIRONMENTAL IMPACT OF COAL MINING

2020 ◽  
Author(s):  
Наталья Алексеевна Бойко ◽  
Наталья Владимировна Ромашева
Keyword(s):  
Environmental Impact ◽  
Organizational Structure ◽  
Coal Mining ◽  
Negative Impact ◽  
Coal Industry ◽  
Coal Production ◽  
Atmospheric Air

Представлена характеристика угольной отрасли России по таким направлениям как организационная структура, объем и регионы добычи, потребители угля. Выявлены положительные тенденции, определены проблемы в развитии угольной промышленности. Исследованы негативное воздействие угольного производство на такие компоненты окружающей среды, как атмосферный воздух, водные ресурсы, земная поверхность. The characteristic of the Russian coal industry in such areas as the organizational structure, volume and regions of production, coal consumers has been presented. Positive trends and problems in the development of the coal industry have been identified. The negative impact of coal production on environmental components such as atmospheric air, water, the earth’s surface has been investigated.

scholarly journals Extraction and exploitation of fossil fuels as the foundation stone of concept of geoecology in Russia

2020 ◽  
Vol 13 (4) ◽  
pp. 550-560
Author(s):  
M. S. Goncharov ◽  
I. M. Rozhkov ◽  
I. A. Larionova
Keyword(s):  
Fossil Fuels ◽  
Environmental Problem ◽  
Negative Impact ◽  
Management Information Systems ◽  
Life Quality ◽  
Coal Industry ◽  
Close Attention ◽  
Industrial Enterprises ◽  
Strategic Solution ◽  
Foundation Stone

Energy sector is considered to be the basis for economy’s growth, improvement of the population’s life quality, maintenance of energy security of the country. However, the activities of some enterprises of the fuel and energy complex have a negative impact on the environment. The problems of geoecology in the field of fuel and energy require close attention. In this article, by the example of coal industry, gives an evaluation of effects industrial enterprises on the environment. Based on the analysis of official statistical sources, the key trends in this area are highlighted. The strategic solution to the environmental problem in the coal industry, according to the authors, is the creation of energy technology and coal-chemical clusters. Geoecology management information systems should be developed for each cluster. The simplest structure of these systems is given in this paper. The next stage of the study should be to set and solve the problem of optimizing the functioning of each cluster and calculate rating estimations.

Perfecting DNA double-strand break repair on transcribed chromatin

2020 ◽  
Vol 64 (5) ◽  
pp. 705-719 ◽  
Author(s):  
Xin Yi Tan ◽  
Michael S.Y. Huen
Keyword(s):  
Dna Damage ◽  
Dna Repair ◽  
Chromosomal Aberrations ◽  
Chromatin Structure ◽  
Genome Stability ◽  
Transcriptional Control ◽  
Strand Break ◽  
Double Strand Break ◽  
Dna Double Strand Break ◽  
Molecular Bases

Abstract Timely repair of DNA double-strand break (DSB) entails coordination with the local higher order chromatin structure and its transaction activities, including transcription. Recent studies are uncovering how DSBs trigger transient suppression of nearby transcription to permit faithful DNA repair, failing of which leads to elevated chromosomal aberrations and cell hypersensitivity to DNA damage. Here, we summarize the molecular bases for transcriptional control during DSB metabolism, and discuss how the exquisite coordination between the two DNA-templated processes may underlie maintenance of genome stability and cell homeostasis.

scholarly journals The Role of Investment and Innovation Activities of Coal Mining Enterprises in Increasing the Level of Environmental Safety of Country and Region

2020 ◽  
Vol 174 ◽  
pp. 04008
Author(s):  
Oksana Sheveleva ◽  
Ekaterina Slesarenko ◽  
Natalia Kudrevatykh ◽  
Maria Kumaneeva
Keyword(s):  
Coal Mining ◽  
Production Efficiency ◽  
Negative Impact ◽  
Environmental Safety ◽  
Coal Industry ◽  
Technical Standards ◽  
Innovation Activities ◽  
Fixed Assets ◽  
Business Entities

The key role of investment and innovation activities of coal industry enterprises in improving the environmental safety of regions specializing in coal mining is substantiated in the article. The coal industry enterprises are recognized by the world scientific community as the absolute leader in the field of negative impact on the environment and industrial production waste generation. In this regard, one of the most important tasks of increasing the investment and innovation activities of coal companies, the introduction of modern technologies should be not only an increase in labor productivity and production efficiency, but also a reduction of anthropogenic impact on the environment. Despite the obviousness of the presented thesis, the trends characterizing the coal industry as the most inert and low-innovative sphere, which follows the path of extensive development, continue to dominate. The insignificant increase in investments in fixed assets, periodically followed by a recession, the reduction of trapped and neutralized air polluting substances, the lack of demand for innovations in the coal industry, as well as their low impact on ensuring compliance with modern technical standards, procedures and rules, bear evidence of low responsibility of business entities and inefficient measures of state economic policy in the field of improving environmental safety in the commodity-dependent regions.

BCR/ABL Kinase Elevates ROS-Mediated Oxidative DNA Damage in CML Stem/Progenitor Cells and Affects the Efficiency and Fidelity of DNA Repair To Induce Genetic Instability.

Blood ◽  
2007 ◽  
Vol 110 (11) ◽  
pp. 34-34
Author(s):  
Margaret Nieborowska-Skorska ◽  
Artur Slupianek ◽  
Tomasz Stoklosa ◽  
Tomasz Poplawski ◽  
Kimberly Cramer ◽  
...  
Keyword(s):  
Dna Damage ◽  
Dna Repair ◽  
Genomic Instability ◽  
Chromosomal Aberrations ◽  
Oxidative Dna Damage ◽  
Point Mutations ◽  
Dna Lesions ◽  
Leukemia Cells ◽  
Abl Kinase ◽  
Imatinib Resistant

Abstract BCR/ABL kinase transforms hematopoietic stem cells (HSCs) to induce chronic myelogenous leukemia in chronic phase (CML-CP), which eventually evolves into fatal blast crisis (CML-BC). CML is a stem cell-derived but a progenitor-driven disease. In CML-CP leukemia stem (LSCs) and progenitor (LPCs) cells reside in CD34+CD38− and CD34+CD38+ populations, respectively, whereas in CML-BC LSCs are found also in CD34+CD38+ population. BCR/ABL kinase stimulates genomic instability causing imatinib-resistant point mutations and chromosomal aberrations associated with progression to CML-BC. Genomic instability may result from enhanced DNA damage and/or aberrant DNA repair mechanisms. We showed that CD34+ stem/progenitor CML cells contain higher levels of reactive oxygen species (ROS) than these from healthy donors (CML-BC>CML-CP>Normal). In addition, ROS were elevated in CD34+CD38− and CD34+CD38+ sub-populations isolated from CML-BC and CML-CP patients in comparison to cells from healthy donor. Higher ROS levels induced more oxidative DNA lesions such as oxidized bases (e.g., 8-oxoG) and DNA double-strand breaks (DSBs). ROS and oxidative DNA damage in CML stem/progenitor cells could be diminished by an antioxidant N-acetyl-cysteine. Moreover, inhibition of ROS by vitamin E reduced the frequency of imatinib-resistant BCR/ABL point mutants and chromosomal aberrations in leukemia cells in SCID mice. Cellular DNA repair systems act to remove DNA damage and ultimately preserve the informational integrity of the genome. Base excision repair (BER) and mismatch repair (MMR) are responsible for removal of oxidized bases. BER was assessed using single- and double-stranded DNA substrates containing 5-OH-U (a derivative of ROS-damaged hydroxy-deoxycytidine). MMR activity was measured by restoration of the expression of GFP from the construct containing T-G mismatch in the start codon. BCR/ABL kinase severely inhibited BER and MMR in cell lines and CD34+ CML cells, and promoted accumulation of point mutations in genes encoding BCR/ABL kinase and Na+/K+ ATPase. Inhibition of BCR/ABL kinase by imatinib restored BER and MMR activities. Oxidized bases, if not repaired, may lead to accumulation of DSBs observed in LSCs and LPCs. DSBs may be processed by homologous recombination (HR), non-homologous and-joininig (NHEJ), and single-strand annealing (SSA). HR represents faithful repair, NHEJ usually produces small deletions, and SSA causes very large deletions. Genome-integrated repair-specific reporter cassettes containing two disrupted fragments of the gene encoding GFP were used where a single DSB induced by I-SceI endonuclease in one of the fragments stimulated HR, NHEJ, or SSA. In general, BCR/ABL kinase enhanced DSBs repair activities, however at the expense of their fidelity. Numerous point mutations were introduced in HR repair products. NHEJ generated larger than usual deletions. SSA, rather rare but very unfaithful, was also induced in BCR/ABL-positive leukemia cells. In summary, BCR/ABL kinase enhanced ROS-mediated oxidative DNA damage in LSCs and LPCs. In addition, BCR/ABL inhibited BER and MMR of usually non-lethal oxidized DNA lesions leading to accumulation of point mutations. Moreover, BCR/ABL kinase stimulated HR, NHEJ and SSA of lethal DSBs, but compromised the fidelity of repair.

scholarly journals The research of environmental-and-economic risks of the coal mining enterprise impact on water resources

2019 ◽  
Vol 134 ◽  
pp. 01019
Author(s):  
Vladimir Mikhailov ◽  
Natalia Kudrevatykh ◽  
Tatiana Tyuleneva
Keyword(s):  
Environmental Management ◽  
Water Resources ◽  
Coal Mining ◽  
Negative Impact ◽  
Practical Importance ◽  
Management Decision ◽  
High Tech ◽  
Protection Measures ◽  
Industrial Enterprises ◽  
Mining Enterprise

Coal mining enterprise is a high-tech complex with a significant negative impact on water resources in the form of wastewater discharge. To improving the environmental safety of enterprises having a negative impact on the aquatic ecosystem, it is necessary to detail its features in order to increase the efficiency of environmental management. The article analyzes known approaches to the definition and features of formation of systems of assessing the negative impact on water resources. On the basis of synthesis of theoretical developments of domestic and foreign researchers` study and analysis the actual data of an industrial enterprise authors researched its negative impact on water resources to form a program of effective water protection measures. This researching has practical importance for large industrial enterprises that have a negative impact on water resources for effective environmental management decision-making.

Associations between DNA Damage, DNA Base Excision Repair Gene Variability and Alzheimer's Disease Risk

2016 ◽  
Vol 41 (3-4) ◽  
pp. 152-171 ◽  
Author(s):  
Dominik Kwiatkowski ◽  
Piotr Czarny ◽  
Monika Toma ◽  
Natalia Jurkowska ◽  
Agnieszka Sliwinska ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Dna Damage ◽  
Dna Repair ◽  
Base Excision Repair ◽  
Excision Repair ◽  
Dna Lesions ◽  
Polymorphic Variants ◽  
Base Excision

Background: Increased oxidative damage to DNA is one of the pathways involved in Alzheimer's disease (AD). Insufficient base excision repair (BER) is in part responsible for increased oxidative DNA damage. The aim of the present study was to assess the effect of polymorphic variants of BER-involved genes and the peripheral markers of DNA damage and repair in patients with AD. Material and Methods: Comet assays and TaqMan probes were used to assess DNA damage, BER efficiency and polymorphic variants of 12 BER genes in blood samples from 105 AD patients and 130 controls. The DNA repair efficacy (DRE) was calculated according to a specific equation. Results: The levels of endogenous and oxidative DNA damages were higher in AD patients than controls. The polymorphic variants of XRCC1 c.580C>T XRCC1 c.1196A>G and OGG1 c.977C>G are associated with increased DNA damage in AD. Conclusion: Our results show that oxidative stress and disturbances in DRE are particularly responsible for the elevated DNA lesions in AD. The results suggest that oxidative stress and disruption in DNA repair may contribute to increased DNA damage in AD patients and risk of this disease. In addition, disturbances in DRE may be associated with polymorphisms of OGG1 and XRCC1.

Long-term efficacy of a new medical device containing Fernblock® and DNA repair enzyme complex in the treatment and prevention of cancerization field in patients with actinic keratosis.

2019 ◽  
Vol 2 (02) ◽  
pp. 80-89
Author(s):  
Blanca De Unamuno Bustos ◽  
Natalia Chaparr´´o Aguilera ◽  
Inmaculada Azorín García ◽  
Anaid Calle Andrino ◽  
Margarita Llavador Ros ◽  
...  
Keyword(s):  
Dna Damage ◽  
Dna Repair ◽  
Medical Device ◽  
Actinic Keratosis ◽  
Statistical Significance ◽  
Dna Lesions ◽  
Enzyme Complex ◽  
Repair Enzyme ◽  
P53 Expression ◽  
Cancerization Field

Actinic keratosis (AKs) are part of the cancerization field, a region adjacent to AKs containing subclinical and histologically abnormal epidermal tissue due to Ultraviolet (UV)-induced DNA damage. The photoproducts as consequence of DNA damage induced by UV are mainly cyclobutane pyrimidine dimers (CPDs). Fernblock® demonstrated in previous studies significant reduction of the number of CPDs induced by UV radiation. Photolyases are a specific group of enzymes that remove the major UV-induced DNA lesions by a mechanism called photo-reactivation. A monocentric, prospective, controlled, and double blind interventional study was performed to evaluate the effect of a new medical device (NMD) containing a DNA-repair enzyme complex (photolyases, endonucleases and glycosilases), a combination of UV-filters, and Fernblock® in the treatment of the cancerization field in 30 AK patients after photodynamic therapy. Patients were randomized into two groups: patients receiving a standard sunscreen (SS) andpatients receiving the NMD. Clinical, dermoscopic, reflectance confocal microscopy (RCM) and histological evaluations were performed. An increase of AKs was noted in all groups after three months of PDT without significant differences between them (p=0.476). A significant increase in the number of AKs was observed in SS group after six (p=0.026) and twelve months of PDT (p=0.038); however, this increase did not reach statistical significance in the NMD group. Regarding RCM evaluation, honeycomb pattern assessment after twelve months of PDT showed significant differences in the extension and grade of the atypia in the NMD group compared to SS group (p=0.030 and p=0.026, respectively). Concerning histopathological evaluation, keratinocyte atypia grade improved from baseline to six months after PDT in all the groups, with no statistically significant differences between the groups. Twelve months after PDT, p53 expression was significantly lower in the NMD group compared to SS group (p=0.028). The product was well-tolerated, with no serious adverse events reported. Our results provide evidence of the utility of this NMD in the improvement of the cancerization field and in the prevention of the development of new AKs.  

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