Comparison of Manual Microscopic and Computer-Assisted Methods for Analysis of Sperm Count and Motility

1996 ◽  
Vol 36 (1) ◽  
pp. 1-7 ◽  
Author(s):  
G. M. Centola
Keyword(s):  
Sperm Count ◽  
Computer Assisted

Impact of supplementing commercial rabbit pellets with fresh Moringa oleifera leaves on semen characteristics in rabbit bucks under tropical climate in Trinidad

2021 ◽  
Author(s):  
Krishna Mohan Kumar
Keyword(s):  
Sperm Motility ◽  
Moringa Oleifera ◽  
Sperm Count ◽  
Control Group ◽  
Computer Assisted ◽  
Group Iii ◽  
Group I ◽  
Significant Difference ◽  
Moringa Oleifera Leaves ◽  
The Impact

Objective This study aimed to evaluate the impact of the dietary supplement of Moringa oleifera leaves (MOL) on semen quality and characteristics in rabbits. Methods Eighteen (n=18) breeding bucks of New Zealand white, of similar age group, were used for the study. Three feeding regimes, (i) 100% commercial rabbit pellets (CRP)-Group I (ii) 90% CRP + 10% fresh MOL on a dry matter (DM) basis – Group II and (iii) 80% CRP + 20% fresh MOL on a DM basis – Group III, were adopted and the trial continued for 21 days. After adaptation to the diet, semen was collected from each buck and subjected to evaluation using a computer-assisted semen analyser. Results In Group III, the sperm count, normal sperm morphology, and sperm motility increased (52.0%) in comparison with the control (Group I; 50.1%). The inclusion of 20% Moringa oliefera in the diet (Group III) caused a significant increase (P<0.05) in semen concentration (Control =136.2 M/mL; Group III=297.2 M/mL). There was no significant difference (P>0.05) in sperm motility and semen volume among the groups. Conclusion The results suggest that supplementing commercial rabbit pellets with 20% fresh Moringa oliefera leaves on a DM basis can improve the quality and characteristics of semen in breeding bucks.

Effect of Panax notoginseng Extracts on Inferior Sperm Motility in vitro

1999 ◽  
Vol 27 (01) ◽  
pp. 123-128 ◽  
Author(s):  
Jung-Chou Chen ◽  
Ming-Xiong Xu ◽  
Leih-Der Chen ◽  
Yan-Nian Chen ◽  
Tsan Hung Chiu
Keyword(s):  
Sperm Motility ◽  
Sperm Count ◽  
Semen Analysis ◽  
Panax Notoginseng ◽  
World Health ◽  
Computer Assisted ◽  
Butanol Extract ◽  
The World ◽  
Health Organization

The purpose of this study was to investigate the effects of Panax notoginseng extracts on inferior sperm motility in vitro. Semen samples were collected from 23 patients with sperm motility between 20% and 40%. The sperm count was over 20 × 106/ml in accordance with the World Health Organization standard. 1.0 mg/ml and 2.0 mg/ml of Panax notoginseng extracts including aqueous extract, n-butanol extract, and polysaccharide fraction on sperm motility and progression were evaluated by computer assisted semen analysis. The results demonstrated that sperm motility as well as progression on inferior sperm motility were enhanced at 1 hour and 2 hours after incubation with all three types of extracts.

scholarly journals Effect of Croton caudatus Geiseler Aqueous Root Extract on Reproductive and Biochemical Parameters in Male Wistar Rats

2020 ◽  
Vol 12 (3) ◽  
pp. 251-60
Author(s):  
Razif Dasiman ◽  
Mastura Abd Malek ◽  
Ebby Anuar Bahari ◽  
Fatin Nadzirah Zakaria ◽  
Nina Keterina Hashim ◽  
...  
Keyword(s):  
Biochemical Parameters ◽  
Sperm Count ◽  
Male Rats ◽  
Seminiferous Tubules ◽  
Computer Assisted ◽  
Root Extract ◽  
Rheumatic Arthritis ◽  
Male Wistar Rats ◽  
Histological Results ◽  
Aqueous Root Extract

BACKGROUND: Croton caudatus Geiseler (CCG), a local herb that empirically has been used as traditional medicine for malaria, fever, convulsions, and rheumatic arthritis. This study aimed to investigate the effect of CCG on reproductive and biochemical parameters in male Wistar rats.METHODS: Twenty-four Wistar male rats were divided randomly into four groups, which were treated with CCG aqueous root extract at the concentration of 0-16 mg/kg, via oral gavage. After 21 days of treatment, blood, sperm, and testes were harvested for analyses. Sperm parameters were evaluated using computer-assisted sperm analyser (Hamilton Thorne Sperm Analyzer), and morphometric analyses of histological changes were performed.RESULTS: CCG extract at 8 and 16 mg/kg significantly (p<0.05) increased the total sperm count, concentration, and motility; whereby, only 16 mg/kg significantly decreased the percentage of abnormal sperm morphology. Testosterone hormones levels were the highest in 4 mg/kg CCG but were slightly decreased in 8 and 16 mg/kg CCG. The histological results showed a significant increase in the germinal epithelial height, decrease of lumen size at 16 mg/kg of CCG extract. No significant changes of alanine aminotransferase (ALT) and creatinine levels in serum from all CCG extract groups. However, the aspartate aminotransferase (AST) level in 8 mg/kg CCG was elevated, and the blood urea nitrogen (BUN) level was decreased in all CCG extract groups.CONCLUSION: In conclusion, the CCG extract at 4 mg/kg has the potential to enhance spermatogenesis activity within the seminiferous tubules in rats without any hepatotoxicity and nephrotoxicity effect on the liver and kidney.KEYWORDS: Croton caudatus Geiseler, rats, spermatogenesis, seminiferous tubules, testis, testosterone

scholarly journals New Insight into Sperm Capacitation: A Novel Mechanism of 17β-Estradiol Signalling

2018 ◽  
Vol 19 (12) ◽  
pp. 4011 ◽  
Author(s):  
Tereza Bosakova ◽  
Antonin Tockstein ◽  
Natasa Sebkova ◽  
Ondrej Simonik ◽  
Hana Adamusova ◽  
...  
Keyword(s):  
Kinetic Analysis ◽  
High Performance ◽  
Acrosome Reaction ◽  
Sperm Count ◽  
Membrane Surface ◽  
Sperm Maturation ◽  
Computer Assisted ◽  
Sperm Capacitation ◽  
17Β Estradiol ◽  
Elevated Rate

17β-estradiol (estradiol) is a natural estrogen regulating reproduction including sperm and egg development, sperm maturation—called capacitation—and sperm–egg communication. High doses can increase germ cell apoptosis and decrease sperm count. Our aim was to answer the biological relevance of estradiol in sperm capacitation and its effect on motility and acrosome reaction to quantify its interaction with estrogen receptors and propose a model of estradiol action during capacitation using kinetic analysis. Estradiol increased protein tyrosine phosphorylation, elevated rate of spontaneous acrosome reaction, and altered motility parameters measured Hamilton-Thorne Computer Assisted Semen Analyzer (CASA) in capacitating sperm. To monitor time and concentration dependent binding dynamics of extracellular estradiol, high-performance liquid chromatography with tandem mass spectrometry was used to measure sperm response and data was subjected to kinetic analysis. The kinetic model of estradiol action during sperm maturation shows that estradiol adsorption onto a plasma membrane surface is controlled by Langmuir isotherm. After, when estradiol passes into the cytoplasm, it forms an unstable adduct with cytoplasmic receptors, which display a signalling autocatalytic pattern. This autocatalytic reaction suggests crosstalk between receptor and non-receptor pathways utilized by sperm prior to fertilization.

scholarly journals Genetic resistance to DEHP-induced transgenerational endocrine disruption

2018 ◽  
Author(s):  
Ludwig Stenz ◽  
Rita Rahban ◽  
Julien Prados ◽  
Serge Nef ◽  
Ariane Paoloni-Giacobino
Keyword(s):  
Endocrine Disruption ◽  
Corn Oil ◽  
Sperm Count ◽  
Inbred Mice ◽  
Genetic Resistance ◽  
Computer Assisted ◽  
Prenatally Exposed ◽  
Transcriptional Responses ◽  
Testicular Dysgenesis Syndrome ◽  
Sperm Analysis

AbstractDi(2-ethylhexyl)phthalate (DEHP) interferes with sex hormones signaling pathways (SHP). C57BL/6J mice prenatally exposed to DEHP develop a testicular dysgenesis syndrome (TDS) at adulthood, but similarly-exposed FVB/N mice are not affected. Here we aim to understand the reasons behind this drastic difference that should depend on the genome of the strain. In both backgrounds, pregnant female mice received per os either DEHP or corn oil vehicle and the male filiations were examined. Computer-assisted sperm analysis showed a DEHP-induced decreased sperm count and velocities in C57BL/6J. Sperm RNA sequencing experiments resulted in the identification of the 62 most differentially expressed RNAs. These RNAs, mainly regulated by hormones, produced strain-specific transcriptional responses to prenatal exposure to DEHP; a pool of RNAs was increased in FVB, another pool of RNAs was decreased in C57BL/6J. In FVB/N, analysis of non-synonymous SNP impacting SHP identified rs387782768 and rs387782768 respectively associated with absence of the Forkhead Box A3 (Foxa3) RNA and increased expression of estrogen receptor 1 variant 4 (NM_001302533) RNA. Analysis of the role of SNPs modifying SHP binding sites in function of strain-specific responses to DEHP revealed a DEHP-resistance allele in FVB/N containing an additional FOXA1-3 binding site at rs30973633 and four DEHP-induced beta-defensins (Defb42, Defb30, Defb47 and Defb48). A DEHP-susceptibility allele in C57BL/6J contained five SNPs (rs28279710, rs32977910, rs46648903, rs46677594 and rs48287999) affecting SHP and six genes (Svs2, Svs3b, Svs4, Svs3a, Svs6 and Svs5) epigenetically silenced by DEHP. Finally, targeted experiments confirmed increased methylation in the Svs3ab promoter with decreased SEMG2 persisting across generations, providing a molecular explanation for the transgenerational sperm velocity decrease found in C57BL/6J after DEHP exposure. We conclude that the existence of SNP-dependent mechanisms in inbred mice may confer resistance to transgenerational endocrine disruption.

422. Data mining: relationship of sperm kinetics and DNA integrity

2008 ◽  
Vol 20 (9) ◽  
pp. 102 ◽  
Author(s):  
K. Osman ◽  
S. Ibrahim ◽  
M. Ismail ◽  
S. Das ◽  
M. Abd Rahman ◽  
...  
Keyword(s):  
Dna Damage ◽  
Damage Identification ◽  
Sperm Count ◽  
Dna Integrity ◽  
Computer Assisted ◽  
Sperm Dna Damage ◽  
Damage Category ◽  
Neutral Comet Assay ◽  
Minimal Damage ◽  
Damage Condition

Intracytoplasmic sperm injection (ICSI) is a popular technique in treating infertile male that bypasses sperm natural selection. Due to this, the occasional and unintentional use of spermatozoa in ICSI with high amount of DNA fragmentation seems to be unpreventable. The objective of this study was to develop single sperm selection technique and in the process to determine the relationship between sperm kinetic parameters and sperm DNA damage. Semen from sexually matured male Boer buck cross species were collected and cryo-preserved. After taking into consideration semen sperm count, sperm were isolated individually in an ELISA plate by diluting semen in extender. Then every sperm’s kinetics was assessed by computer-assisted sperm analyzer (CASA) while neutral comet assay was used to quantitate and categorise its DNA damage condition. DNA damage was categorised from minimal damage (category 0) to extensive damage (category 4). Relationship between CASA parameters and DNA damage category of 490 sperms was determined using a Classification and Regression modelling (C&R). A total of 208 sperm data was used to generate a suitable C&R model. A further 250 sperm data was then used to determine accuracy of the model. Results obtained indicated that VSL, WOB and VCL were important factors in determining the overall condition of a particular sperm. A low value of VSL would indicate minimal DNA damage. Identification of higher category of DNA damage would require combination assessment of VSL, WOB and VCL. Accuracy of the developed C&R model was at 83.6%. Based on the above procedure it has been shown that sperm kinetics and DNA integrity can be considered together in selecting potential sperm for ICSI procedure.

scholarly journals A collective tracking method for preliminary sperm analysis

2019 ◽  
Vol 18 (1) ◽  
Author(s):  
Sung-Yang Wei ◽  
Hsuan-Hao Chao ◽  
Han-Ping Huang ◽  
Chang Francis Hsu ◽  
Sheng-Hsiang Li ◽  
...  
Keyword(s):  
Image Processing ◽  
Sperm Count ◽  
Semen Analysis ◽  
Least Square ◽  
Image Processing Technique ◽  
Video Frame ◽  
Computer Assisted ◽  
Tracking Method ◽  
Sperm Analysis ◽  
Point Tracking

Abstract Background Total motile sperm count (TMSC) and curvilinear velocity (VCL) are two important parameters in preliminary semen analysis for male infertility. Traditionally, both parameters are evaluated manually by embryologists or automatically using an expensive computer-assisted sperm analysis (CASA) instrument. The latter applies a point-tracking method using an image processing technique to detect, recognize and classify each of the target objects, individually, which is complicated. However, as semen is dense, manual counting is exhausting while CASA suffers from severe overlapping and heavy computation. Methods We proposed a simple frame-differencing method that tracks motile sperms collectively and treats their overlapping with a statistical occupation probability without heavy computation. The proposed method leads to an overall image of all of the differential footprint trajectories (DFTs) of all motile sperms and thus the overall area of the DFTs in a real-time manner. Accordingly, a theoretical DFT model was also developed to formulate the overall DFT area of a group of moving beads as a function of time as well as the total number and average speed of the beads. Then, using the least square fitting method, we obtained the optimal values of the TMSC and the average VCL that yielded the best fit for the theoretical DFT area to the measured DFT area. Results The proposed method was used to evaluate the TMSC and the VCL of 20 semen samples. The maximum TMSC evaluated using the method is more than 980 sperms per video frame. The Pearson correlation coefficient (PCC) between the two series of TMSC obtained using the method and the CASA instrument is 0.946. The PCC between the two series of VCL obtained using the method and CASA is 0.771. As a consequence, the proposed method is as accurate as the CASA method in TMSC and VCL evaluations. Conclusion In comparison with the individual point-tracking techniques, the collective DFT tracking method is relatively simple in computation without complicated image processing. Therefore, incorporating the proposed method into a cell phone equipped with a microscopic lens can facilitate the design of a simple sperm analyzer for clinical or household use without advance dilution.

Semen collection from mice: electroejaculation

2002 ◽  
Vol 14 (6) ◽  
pp. 363 ◽  
Author(s):  
R. T. Tecirlioglu ◽  
E. S. Hayes ◽  
A. O. Trounson
Keyword(s):  
Sperm Count ◽  
Sine Wave ◽  
Computer Assisted ◽  
Probe Type ◽  
Sound Card ◽  
Function Generator ◽  
Cba Mice ◽  
Semen Collection ◽  
Device Type ◽  
Pentobarbitone Sodium

The effects of device type (electrostimulator, function generator or computer-generated waveforms), waveform (square, triangle or sine wave), probe type (ring or strip) and anaesthetic compound (ketamine/xylazine combination or pentobarbitone sodium) were investigated on electroejaculation (EEJ) responses of C57Bl × CBA and C57Bl/6J mice. Ejaculates were analysed for total sperm count and motility variables using computer-assisted sperm analyses. Automated computer-generated waveforms delivered through a sound card were more effective and reproducible compared with waveforms generated by function generator and electrostimulator. Sine waves and triangle waves were found to be more effective in producing ejaculate than square waves. As an anaesthetic, pentobarbitone sodium tended to outperform ketamine/xylazine across waveforms and strains. Strip probes failed to produce any ejaculate regardless of the device or waveform employed. Sperm obtained by EEJ exhibited poor motility and C5Bl/6J mice had lower motility variables than C57Bl × CBA mice.

Effects of Shao-Fu-Zhu-Yu-Tang on Motility of Human Sperm

2003 ◽  
Vol 31 (04) ◽  
pp. 573-579 ◽  
Author(s):  
Ching-Chiang Yang ◽  
Jung-Chou Chen ◽  
Guang-Wei Chen ◽  
Yi-Shuan Chen ◽  
J.G. Chung
Keyword(s):  
Chronic Prostatitis ◽  
Sperm Count ◽  
Semen Analysis ◽  
Human Sperm ◽  
Computer Assisted ◽  
Activity Levels ◽  
Human Semen ◽  
Before And After ◽  
Acrosin Activity

Shao-Fu-Zhu-Yu-Tang (SFZYT) is reportedly beneficial to sperm. In this study, we examined sperm acrosomal activity and serum free radical changes to evaluate the possible mechanism of SFZYT. A clinical study evaluated the sperm count and motility in 36 patients with chronic prostatitis before and after treatment for 60 days. The results revealed a significant increase in sperm motility after treatment as evaluated by computer-assisted semen analysis (17.27 ± 9.00 versus 28.29 ± 10.00, p < 0.01). An increase in sperm quantity and quality was observed by count and morphology with a high-powered intravital microscope. To gain an understanding of the mechanisms that caused this effect, we assessed sperm acrosin activity levels before (10.6 μ lu /106) and after medication (28.6 μ lu /106)( p < 0.01). The levels of the free radicals was relatively higher before medication, 2144, compared to a normal value of 780 after medication ( p < 0.01). In conclusion, SFZYT increased the motility and quality of human semen and this increase is related to an increase in sperm acrosin activity. SFZYT also works as a sperm antioxidant and antiaging agent.

P–123 How to develop accurate Computer Assisted Sperm Analysis (CASA) AI in the absence of protocol standardization and abundance of human error when performing semen analyses?

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
Z Simon ◽  
R Maillot ◽  
M Monteiro ◽  
S Rogers ◽  
A Mania ◽  
...  
Keyword(s):  
Relative Error ◽  
Human Error ◽  
Sperm Count ◽  
Semen Analysis ◽  
Robotic System ◽  
Semen Sample ◽  
Computer Assisted ◽  
Who Guidelines ◽  
Manual Analysis ◽  
Protocol Standardization

Abstract Study question How can an automation & artificial intelligent tools be developed to perform according to WHO recommendations? Summary answer Developing CASA performs at &lt; 20% error margin requires AI trained with high quality datasets and a robotic system adheres to WHO guidelines. What is known already A survey of 40 andrology laboratories, in 22 countries, revealed that &gt; 90% had nonconformities in correct use of equipment, standardisation of protocols and quality control, leading to a lack of compliance to WHO protocols. Conventional CASA systems can standardize analysis, but controversy has occurred due to differences between manual and automated analyses stemming from: 1) all cells in a semen sample are detected including debris; 2) protocol variation when compared to top-notch manual analysis. The first point can be addressed by AI. The second point can be addressed by robotics designed to adhere to WHO guidelines. Study design, size, duration A mojo AISA (AI-powered semen analysis) system was placed in four clinical laboratories mentioned above capturing images of over 300 samples, one million images were generated over a course of 2 years. Mojo AISA’s AI was trained on data collected from the four clinics using robotic system is developed according to WHO guidelines. Participants/materials, setting, methods For an AI to detect sperm accurately, sperm samples were captured using mojo AISA smart microscopy and then the extracted sperm images expertly annotated. To evaluate the system-ability for semen analysis, fresh sample were analysed for concentration and motility by a manual operator and compared to a mojo AISA test. Main results and the role of chance To train the sperm detection AI, representative sperm images were carefully captured using mojo AISA and processed according to the following criteria: the number of images and videos to train and to test the model: 50,000 spermatozoon head and tails with various variations the variety of images: data used to train the AI has to be representative of the population that will undergo the analysis: 1) wide concentration ranges from 0 to 300 M/ml, 2) high and low density of debris and cells, 3) Presence of slight aggregations careful and precise annotation: expert andrology scientists annotated sperm images and identify objects to exclude, such as debris in seminal plasma, Mojo AISA is an attempt strictly build CASA AI system to WHO-guidelines. The marriage of AI and robotics automation has shown a promising results to mimic humans when measuring a semen sample and attempt to obtain results comparable to the manual analysis. mojo AISA’s performance improved three-fold (from 0,85 to 0,95 Pearson sperm count correlation and from &gt;100% means relative error to 25% mean relative error). Limitations, reasons for caution Lack of standardization for semen analysis laboratory process globally is a bottleneck towards building a robust multi-center study, on-site CASA testing and generating an actionable data pool for studying the causes behind male fertility declineWider implications of the findings: Key learnings for parties advancing developing AI based on images and videos for application in the fertility space. Trial registration number Not applicable

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