Pattern of Protein Profiles of Reproductive Organs after Induced Bilateral Cryptorchidism in Albino Rats

he present work was designed to investigate the reproductive toxicity induced by oral administration of chlorpyrifos (CPF), cypermethrin (CYP) and their combination in adult male albino rats. Forty mature male albino rats were separated into four groups (10 each), the first group was used as control, while second, third and fourth groups received orally 1/20 LD50 of CPF (10 mg/kg b.wt), 1/20 LD50 of CYP (17.22 mg/kg b.wt) and 1/40 LD50 of CPF plus 1/40 LD50 of CYP (5 mg/kg b.wt CPF plus 8.61 mg/kg b.wt CYP) respectively for 26 days. The results revealed that exposure to CPF and/or CYP induced a significant decrease in the reproductive organs weight. Moreover, a significant decrease in spermatic picture (sperm cell concentration and viability) was observed with high percent of sperm abnormalities. Serum levels of testosterone and pituitary gonadotropins (FSH and LH) have been declined significantly in all treated groups. Significant elevations were observed in malondialdehyde and nitric oxide concentrations, while antioxidant enzymes superoxide dismutase and glutathione-S-transferase activities were decreased significantly as a result of induced oxidative stress. A significant drop in prostatic acid phosphatase activity was observed. Additionally, the results showed some histopathological alterations in the reproductive organs as well as neurological lesions in brain and pituitary glands. In conclusion, CPF and CYP induce deleterious effects on reproductive efficiency of male rats which reflect more obvious impacts when both combined

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Effect of induced bilateral cryptorchidism on the carbohydrate metabolism of reproductive tissues in albino rats

Archives Internationales de Physiologie et de Biochimie ◽

10.3109/13813458309067987 ◽

1983 ◽

Vol 91 (5) ◽

pp. 405-410 ◽

Cited By ~ 1

Author(s):

K. Venkatarami Reddy ◽

N. Geethanjali ◽

Y. Dhananjaya Reddy ◽

P. Reddanna ◽

S. Govindappa

Keyword(s):

Carbohydrate Metabolism ◽

Albino Rats ◽

Reproductive Tissues ◽

Bilateral Cryptorchidism

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Ameliorative Effects of Honey, Propolis, Pollen, and Royal Jelly Mixture against Chronic Toxicity of Sumithion Insecticide in White Albino Rats

Molecules ◽

10.3390/molecules25112633 ◽

2020 ◽

Vol 25 (11) ◽

pp. 2633 ◽

Cited By ~ 1

Author(s):

Atef M.K. Nassar ◽

Yehia M.M. Salim ◽

Khalid S.A. Eid ◽

Hazem M. Shaheen ◽

Abdullah A. Saati ◽

...

Keyword(s):

Royal Jelly ◽

Corn Oil ◽

Ethylenediaminetetraacetic Acid ◽

Reproductive Organs ◽

Protective Role ◽

Albino Rats ◽

Total Protein Content ◽

Glutathione S Transferase ◽

Significant Toxicity

Sumithion (Fenitrothion) (SUM) is an organophosphorus insecticide used to combat a wide variety of plant pests. Exposure to SUM causes significant toxicity to the brain, liver, kidney, and reproductive organs through, for example, binding to DNA, and it induces DNA damage, which ends with oxidative stress. Therefore, the present study aimed to examine the protective role of bee products: a mixture of honey, propolis, palm pollen, and royal jelly (HPPJ) against SUM-induced toxicity. Twenty-four male albino rats (Rattus norvegicus) were classified into four groups, each containing six rats: control (corn oil), SUM (85 mg/kg; 1/20 LD50), HPPJ, and SUM + HPPJ once daily for 28 consecutive days. Blood samples were gently collected in sterilized ethylenediaminetetraacetic acid (EDTA) tubes for blood picture analyses and tubes without anticoagulant for serum isolation. Serum was used for assays of enzymatic and biochemical characteristics. The results revealed that SUM increased the weights of the liver, kidney, and brain as well as the enzymatic activity of glutathione peroxidase (GP), serum superoxide dismutase (SOD), and glutathione-S-transferase (GST). Additionally, SUM significantly increased the activity of lactate dehydrogenase (LDH), alkaline phosphatase (ALP), and γ-glutamyltransferase (γ-GT) and glucose, uric acid, and creatinine contents, while decreasing the acetylcholine esterase (AChE) activity and total lipids and total protein content. Furthermore, because of the inclusion of phenolic, flavonoids, terpenoids, and sugars, the HPPJ mixture counteracted the hematological, renal, and hepatic toxicity of SUM exposure.

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Methotrexate and Leucovorin Exposure Modulates Biochemical Markers in Female Accessory Reproductive Organs of Albino Rats

Acta Endocrinologica (Bucharest) ◽

10.4183/aeb.2012.369 ◽

2012 ◽

Vol 8 (3) ◽

pp. 369-386

Author(s):

Sritulasi Karri

Keyword(s):

Biochemical Markers ◽

Reproductive Organs ◽

Albino Rats

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Testicular Morphological Changes Produced by Fluoroquinolones in Adult Male Albino Rats

Annals of King Edward Medical University ◽

10.21649/akemu.v23i2.1573 ◽

2017 ◽

Vol 23 (2) ◽

Author(s):

Rubina Iqbal ◽

Saud Iqbal ◽

Iram Atta

Keyword(s):

Adult Male ◽

Leydig Cells ◽

Morphological Changes ◽

Sperm Count ◽

Research Work ◽

Testicular Tissue ◽

Reproductive Organs ◽

Seminiferous Tubules ◽

Control Group ◽

Albino Rats

AbstractObjectives: The objective of this research work was to observe the testicular morphological changes produced by fluoroquinolones in the reproductive organs of adult male albino rats, and to see whether these changes are reversible after discontinuation of the drugs.Materials and Method: Eighty adult male albino rats weighing 200 – 300 gms were randomly selected and divided into four groups i.e. A, B, C & D, having 20 animals in each group. A, B & C, were the experimental groups & D served as control group. All the groups were further divided into sub groups 1 & 2. Three fluoroquinolones i.e. Ciprofloxacin (135 mg / kg / day), Ofloxacin (75 mg / kg / day) & Enoxacin (12.5 mg / kg/ day) were given to the groups A, B & C respectively for 42 days. Animals of group D received dis-tilled water only. Animals of sub groups A1, B1, C1 &D1 were sacrificed on 42nd day and testicular tissue was obtained for morphological study. Animals of subgroups A2, B2, C2 & D2 were sacrificed on 84th day and testicular tissue for morphological changes was taken. No of leydig cells, height of epithelium and diameter of seminiferous tubules were taken as experimental parameters for morphological changes.Results: The study indicated statistically significant (P < 0.05) decrease in height of epithelium, diameter of seminiferous tubules and no. of leydig cells in experimental groups as compared to the control groups.Conclusion: The changes observed in morphology could lead to decrease in sperm count and testosterone levels. This study suggests gonadotoxic potentials of fluoroquinolones and adds concern to the indiscriminate and widespread use of fluoroquinolones and recommends more rational use of these drugs.

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Evaluation of potentials of aqueous ethanolic extract of Psidium guajava on reproductive functions of male Albino rats

Sokoto Journal of Veterinary Sciences ◽

10.4314/sokjvs.v19i3.2 ◽

2021 ◽

Vol 19 (3) ◽

pp. 174-181

Author(s):

S.A. Abwage ◽

S.T. Agu ◽

S.A. Saganuwan ◽

A.H. Abu

Keyword(s):

Body Weight ◽

Leaf Extract ◽

Ethanolic Extract ◽

Psidium Guajava ◽

Reproductive Organs ◽

Sperm Parameters ◽

Albino Rats ◽

Reproductive Parameters ◽

Aqueous Ethanolic Extract ◽

Ethanolic Leaf Extract

The therapeutic utilization of plants for managing several diseases by people of all continents, especially Africa, is as old as tradition. The objective of this study was to evaluate the potentials of aqueous ethanolic leaf extract of Psidium guajava on male reproductive parameters. Twenty-four male Albino rats were randomly assigned into four groups of six rats per group. Rats in the control group were administered Tween 20®. Rats in groups 2, 3, and 4 were administered Psidium guajava aqueous ethanolic leaf extract orally at the doses of 100, 200, and 400 mg/kg body weight, respectively, once daily for 60 days. The body weights of the rats were determined at the beginning and end of the experiment. Sperm parameters and some reproductive organs weight of each rat was also determined, some organs were collected for histopathology. Assay for follicle-stimulating hormone, luteinizing hormone, and testosterone was done using Enzyme-Linked Immunosorbent methods. Some reproductive organs were collected for histopathological analyses. The results showed a dose-dependent increase (p<0.05) in the weight of male reproductive organs, sperm parameters, and hormones. The extract significantly increased (P < 0.05) serum testosterone in the group treated 400 mg/kg body weight when treated groups were compared with control. Furthermore, the results of FSH and LH revealed a significant (p < 0.05) increase when the treated groups were compared with the control. The histopathological analysis did not reveal any form of damage to the architectural integrity of the testis. However, there was degenerating germinal epithelium in the group administered 400 mg/kg of the aqueous ethanolic leaf extract of P. guajava. Conclusion: aqueous ethanolic extract of P. guajava has positive effects on male reproductive parameters.

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Effect of Phyllanthus amarus on Some Reproductive Indices of Male Albino Rats

Journal of Applied Life Sciences International ◽

10.9734/jalsi/2019/v20i130076 ◽

2019 ◽

pp. 1-8

Author(s):

P. B. Ekpo ◽

N. E. Edu ◽

A. J. Umoyen ◽

T. L. Thomas ◽

S. O. Abraham

Keyword(s):

Medicinal Plants ◽

Sperm Count ◽

Reproductive Organs ◽

Male Rats ◽

Albino Rats ◽

Phyllanthus Amarus ◽

Experimental Animals ◽

Treatment Groups ◽

Randomized Design ◽

Group B

Background: Medicinal plants have been a good source of drugs for humans, but chronic and prolong use of medicinal plants like Phyllanthus amarus for the treatment of malaria and other disorders are issues of concerns. This study evaluated the effect of Phyllanthus amarus on reproductive organs and sperm parameters in albino rats. Materials and Methods: Twenty-four healthy male albino rats of 12 weeks old were assigned into four groups with six rats in each group using a Completely Randomized Design (CRD). The experimental animals were orally treated with Phyllanthus amarus. Group A served as the control and was given only water and feed; Group B, C and D received 100 mg/kgBW, 200 mg/kgBW and 300 mg/kgBW of Phyllanthus amarus respectively. Data obtained were analyzed using analysis of variance (ANOVA). The treatments lasted for a period of 65 days after two weeks of acclimatization. Results: The results showed statistically significant (p<0.05) reduction in weight of testes and epididymes, sperm motility, sperm viability, sperm count and sperm head abnormalities in male rats treated with Phyllanthus amarus when compared to the control. The sperm pH was not significantly (p>0.05) affected by Phyllanthus amarus among the different treatment groups in the experimental animals. Conclusion: Findings from the present study indicate that Phyllanthus amarus possesses a dose-dependent anti-fertility activity in amale albino rats under a sub-chronic course of administration.

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