scholarly journals Microbial analysis of locally prepared vended dishes in owerri municipality of imo state, Nigeria

2020 ◽  
Vol 3 (10) ◽  
pp. 239-243
Author(s):  
Mercy Adaramaja. F
Keyword(s):  
Total Coliform ◽  
Food Samples ◽  
Viable Count ◽  
Gas Production ◽  
Total Viable Count ◽  
Mould Growth ◽  
White Rice ◽  
Imo State ◽  
Microbial Analysis ◽  
High Sense

A study was carried out to access the microbial analysis of locally prepared vended dishes in Owerri municipality of Imo state. Five zones namely: Prefab and Aladinma areas are represented as Zone A while Ikenegbu and Wethdral areas, Douglas area, School road and Tetlow areas and Worldbank area represented Zone B, C, D and E respectively. The food samples collected includes; rice, spagehetti, beans and white stew. The result obtained revealed that the total viable count in zone A contained 11.42 × 103, 9.5 × 103, 4.4 × 104 and 10.0 × 105 (Cfu/mL) for white rice, spaghetti, beans and stew while those of zone B are 9.0× 103, 7.70 × 103, 2.5× 104 and 6.0 × 105 (Cfu/mL) for white rice, spaghetti, beans and stew respectively. Zone C stew (10.4 ×103 cfu/mL), spaghetti (2.0× 104 cfu/mL), beans (3.3× 104 cfu/mL) and stew (10.3× 104 cfu/mL). Zone D stew (8.0 × 103 cfu/mL), spaghetti (1.5× 104 cfu/mL), beans (1.4× 104 cfu/mL) and stew (4.0×105cfu/mL). Zone E stew (12.55 × 103 cfu/mL), spaghetti (6.3× 103 cfu/mL), beans (6.0× 104 cfu/mL) and stew (4.5×105cfu/mL). Total coliform count shows no gas production in Durbam tube in all the zones, no mould were also observed in all the samples. Generally, mould growth on food is evidence deterioration. It was concluded that the zones maintained high sense of hygiene.

Investigation of microbial contamination of Powdered Infant Formula during different storage periods after opening

2019 ◽  
Vol 10 (03) ◽  
Author(s):  
Mohammed Khalid Al-atrash
Keyword(s):  
Infant Formula ◽  
Environmental Protection Agency ◽  
Microbial Contamination ◽  
Total Coliform ◽  
Viable Count ◽  
Risk Level ◽  
Powdered Infant Formula ◽  
Total Viable Count ◽  
Coliform Count ◽  
Significant Difference

The present study was carried out to knowing effect different storage periods of the microbial quality for the Powdered Infant Formula (PIF) after opening the tin, and ensuring from the safety note (after opening, use within 3 weeks). Thirty (30) samples of (PIF) from category (1 – 6 months) in five different types are collected from pharmacies and local markets in Baquba city / Iraq, which are used as substitutes for breast milk during the first day of opening the tin powders such as Total viable count, Total coliform count, Salmonella count and Yeast and Molds count. These experiments repeated at each week of same samples within (5) weeks. Results were obtained at opening the tin, Total viable count (less than 0.05 1.0 x 103 ±1.5x10 CFU/g) were significantly higher than Total Coliform count (less than 0.05 ± 0.3 x 10 CFU/g) and Total Salmonella count (less than 0.05 0 x 10 CFU/g) and Yeasts and Molds (less than 0.05 ± 0.3 x 10 CFU/g). while results obtained at fifth week were (less than 0.05 8.8 x 103 ±5.5x102 CFU/g), (less than 0.05 0.9 x 102 ± 0.4x101 CFU/g), (less than 0.05 0 x 10 CFU/g), (less than 0.05 9.5 x 10 ± 1.2x101 CFU /g) respectively. All samples of (PIF) having non-significant difference. These results compared to Iraqi Quality Standards (IQS), all the results from the opening samples to fifth week were within the range of IQS and USA Environmental Protection Agency (USEPA) and as indicates the hygienic condition of (PIF) without risk level for human health. also observed increase in microbial contamination in each week because increase the moisture content for powdered milk. Can be used more than 3 weeks after opening if stored in good conditions with good hygienic practices during milk preparation.

scholarly journals Effect of Antibacterial Agents on the Microbial Flora of Some Fruits and Vegetables.

2020 ◽  
pp. 299-304
Author(s):  
Omorodion Nnenna ◽  
Oriji Gift
Keyword(s):  
Fruits And Vegetables ◽  
Antimicrobial Agents ◽  
Reduction Rate ◽  
Total Coliform ◽  
Viable Count ◽  
Microbiological Analysis ◽  
Total Viable Count ◽  
Coliform Count ◽  
Microbiological Methods ◽  
Bacillus Spp

Six samples of fruits (apple, banana, mango, orange, pineapple and watermelon) and six samples of vegetables (cabbage, carrot, cucumber, lettuce, potato and tomato) were purchased from wokem market in Choba town PH, Rivers State and microbiological analysis were carried out these fruits and vegetables samples and the effects of the antimicrobial agents (Ethanol and Hydrogen peroxide) on the microbial load. The Total viable count, Total coliform count and Escherichia count were determined via standard microbiological methods. The Total viable count ranged from 4.1x10106 to 1.0x107 cfu/g Total coliform count from 4.5x107 to 1.5x106 cfu/g nd the E.coli count ranged from 5.1x102 to 1.0x102 cfu/g. The Total viable count for apple ranged between to 5.1 x107 to 2.07x109 , Cabbage 1.45x109 to 4.9x107 , banana 1.10x109 to 5.9x107 , cucumber 1.82x108 to 6.0x107. The microorganisms isolated were Escherichia coli 42%, Staphylococcus aureus 30%, Pseudomonas spp 18%, Bacillus spp,6% and Serratia spp, 2%. Hydrogen perioxide had a high pathogen reduction rate than ethanol.The isolation of E coli and other pathogenic microorganisms indicates the presences of faecal contamination and the poor hygienic production, storage and transport system. Fruits and vegetables should be properly handled from their seeds planted in the farm to the matured ones sold in the market

scholarly journals Nutritional Benefit, Acceptability and Safety of Cookies from Blanched Coconut and Wheat Flour

2020 ◽  
pp. 94-102
Author(s):  
Abiodun Omowonuola Adebayo- Oyetoro ◽  
Okereke Jane A. ◽  
Ogunnubi, Ebunoluwa I. ◽  
Ukwenya, Anita A. ◽  
Ogundipe, Flora O. ◽  
...  
Keyword(s):  
Wheat Flour ◽  
Calorific Value ◽  
Viable Count ◽  
Total Viable Count ◽  
Crude Fibre ◽  
Standard Methods ◽  
Sensory Score ◽  
Microbial Analysis ◽  
Level Of Significance ◽  
Flour Blends

Cookies were made from wheat and blanched coconut flour blends in ratios 90:10, 85:15, 80:20, 75:25, 70:30, 60:40 and 55:45 respectively with 100% wheat as control. Proximate, sensory and microbial analysis of the cookies were assessed using standard methods and established level of significance difference (p<0.5). Results showed that moisture, crude fibre, fat and calorific value ranged between 5.72%-7.80%, 5.77%-7.80%, 9.81-17.21% and 197.08-312.85 Kcal/100g respectively showing increased parameters with increased substitution levels. Carbohydrate and protein ranged from 49.27%-78.21% and 12.69%-15.66% respectively. Mean sensory score for colour, crunchiness and flavor ranged between 7.30-8.50, 7.01-8.02 and 6.30-8.50 respectively while sample 70:30 was the most acceptable in most of the parameters assessed. Total viable count ranged from 1.4x104 cfu/g- 2.5x104 cfu/g between 2nd -5th week of storage while most samples had no fungi growth for the five weeks except for sample 60:40 and 55:45 with growth ranging from 4.0-7.1x104 Cfu/g. Safe and nutritious cookies can be made from wheat-blanched coconut flour.

scholarly journals Public Health Safety in Relation to Microbiological Quality of Freshly Drawn Cow’s Milk in Bangladesh

2014 ◽  
Vol 12 (2) ◽  
pp. 231-236
Author(s):  
R Khaton ◽  
MA Hasnat ◽  
S Rahman ◽  
MM Rahman
Keyword(s):  
Public Health ◽  
Escherichia Coli ◽  
Pathogenic Bacteria ◽  
Total Coliform ◽  
Viable Count ◽  
Microbial Quality ◽  
Total Viable Count ◽  
Coliform Count ◽  
Milk Samples

The aim of the present study was to determine the microbial quality of freshly drawn cow’s milk belonging to the arsenic affected and non-affected areas. In the present work 25 milk sample were collected from five different arsenic affected and non-affected areas. The analysis comprised enumeration of total viable count (TVC), total coliform count (TCC) and total staphylococcal count (TSC) for the determination of sanitary quality. The highest total viable count, total coliform count and total staphylococcus count were log 5.894 ± 0.221, log 2.832± 0.129 and log 2.898 ± 0.162 respectively. Staphylococcus spp, Escherichia coli, Pseudomonas spp and Bacillus spp were isolated from the milk samples. Among them Escherichia coli were isolated from the maximum milk samples. It is found that the level of microbial quality in terms of TVC, TCC and TSC were high in arsenic affected areas than arsenic non-affected area. It also found that TVC and TCC were positively correlated with each other. This survey indicates that most of the raw milk samples were not satisfactory in course of public health standard as some pathogenic bacteria were detected from these samples and it is necessary to improve the hygienic practices required for the handling and processing of milk.DOI: http://dx.doi.org/10.3329/bjvm.v12i2.21297 Bangl. J. Vet. Med. (2014). 12 (2): 231-236 

Evaluation of steam-ultrasound decontamination on naturally contaminated broilers through the analysis of Campylobacter, total viable count, and Enterobacteriaceae

2021 ◽  
Author(s):  
hanieh musavian ◽  
Tariq M. Butt ◽  
Aaron Ormond ◽  
David Keeble ◽  
Niels H. Krebs
Keyword(s):  
Viable Count ◽  
Ultrasound Treatment ◽  
Total Viable Count ◽  
Continuous Processing ◽  
Log Reduction ◽  
Surface Areas ◽  
Ultrasound Application ◽  
Poultry Processing ◽  
Microbial Analysis ◽  
Skin Samples

Combined steam-ultrasound process was investigated for decontamination of freshly slaughtered broilers. Combined steam-ultrasound was delivered simultaneously through specially designed nozzles. The nozzles were installed inside of a constructed machine that allowed for continuous processing. The aim of this study was to evaluate the decontamination effect of the steam- ultrasound application with a capacity of 10,500 birds/hour on naturally contaminated broilers, using three different skin sampling areas for microbial analysis (back, breast and the neck skin). Microbial analysis of Campylobacter , Enterobacteriaceae and Total Viable Count (TVC), was performed pre- and -post steam-ultrasound treatment. A total of 648 skin samples were analyzed for Campylobacter and a total of 216 samples were analyzed for Enterobacteriaceae and TVC. R esults showed significant (p&lt;0.001) Campylobacter reductions of 0.8 log, 1.1 log and 0.7 log, analyzed from skin samples taken from the back, breast and the neck, respectively. Significant reductions of Enterobacteriaceae (p&lt;0.001) by 1.6 log, 1.9 log and 1.1 log and significant reductions of TVC (p&lt;0.001) by 2.0 log, 2.4 log and 1.3 log were found on back, breasts and neck, respectively. Refrigeration effect on Campylobacter numbers pre- and post- steam-ultrasound treatment and incubation at 4°C for eight days was determined in a small trial with 12 samples. Results showed significant (p&lt;0.01) reductions of 0.9 log analyzed on breast skin samples, and 0.7 log reduction (p&lt;0.05) on neck skin samples. Results in this study showed that significant bacteria reduction was achieved on three different surface areas on broilers at a slaughter-speed of 10,500b/h with temperatures over 80°C. The rapid treatment of less than 1.5s exposure time per bird chamber, makes this technology potentially suitable for modern and fast poultry processing lines.

Validation of the Micro Biological Survey Method for Total Viable Count and E. coli in Food Samples

2011 ◽  
Vol 6 (11) ◽  
pp. 951-962 ◽  
Author(s):  
Giorgia Bottini ◽  
Francesca Losito ◽  
Alessio De Ascenti ◽  
Francesca Romana Priolisi ◽  
Alberto Mari ◽  
...  
Keyword(s):  
Food Samples ◽  
Viable Count ◽  
Survey Method ◽  
Total Viable Count ◽  
E Coli ◽  
Biological Survey

scholarly journals Microbial analysis of raw and pasteurized milk from selected areas of Dinajpur, Bangladesh

2015 ◽  
Vol 1 (2) ◽  
pp. 292-296 ◽  
Author(s):  
Md Amirul Hasan ◽  
Md Aminul Islam ◽  
Mohammad Showkat Mahmud ◽  
ASM Ashab Uddin ◽  
Shamim Ahmed
Keyword(s):  
Microbial Contamination ◽  
Raw Milk ◽  
Total Coliform ◽  
Viable Count ◽  
Hygienic Condition ◽  
Pasteurized Milk ◽  
Legal Standards ◽  
Milk Samples ◽  
Microbial Analysis ◽  
The Government

Milk is highly vulnerable to microbial contamination and consequently is easily perishable. The aim of the present study was microbial analysis of raw and pasteurized milk. For this, a total of 32 milk samples (12 from raw milk and 20 from pasteurized milk) were collected from 8 sources. Among them three were raw (R1 to R3) sources and rest five were pasteurized milk (P1 to P5) sources. Each of the collected samples was investigated during the period from May to December, 2012. All milk samples were subjected to total viable count (TVC), total coliform count (TCC), Total Staphylococcus count (TSC) and Gram’s staining to determine the loads of microbes in raw and pasteurized milk. Total viable counts (TVC) range of 12 raw milk samples (R, R2 and R3) were 1.3 x106 to 7.4 x105 cfu/ml. The presence of Escherichia coli in the raw milk samples were from 2.3 x102 to 9.4 x102 cfu/ml, but the presence of Staphylococcus were from 5.9 x102 to 7.9 x102 cfu/ml. Whereas, the range of TVC for five brands of pasteurized milk (P1, P2, P3, P4, and P5) were from 1.8 x104 to 9.8 x104 cfu/ml, TSC were from 2.8 x10 to 8.6 x102 cfu/ml and TCC from 1.01 x102 to 9.1 x10 cfu/ml. Therefore, it can be concluded that high counts of bacteria were found in raw milk and pasteurized milk. The government therefore should conduct frequent inspection of the marketed milks to check whether they meet the minimum legal standards and should monitor the overall hygienic condition surrounding the production and handling of milk. Realistic standards for the raw milks need to be devised and appropriate training should be given to the raw milk producers in hygienic handling of milk.Asian J. Med. Biol. Res. June 2015, 1(2): 292-296

scholarly journals Bacterial Analysis of Different Types of Ice Creams from Dhaka City

2016 ◽  
Vol 29 (1) ◽  
pp. 41-43
Author(s):  
Sabrina Akter ◽  
Sangita Ahmed
Keyword(s):  
Fecal Coliform ◽  
Ice Cream ◽  
Good Manufacturing Practice ◽  
Total Coliform ◽  
Viable Count ◽  
Dhaka City ◽  
Total Viable Count ◽  
Coliform Count ◽  
Different Types ◽  
Ice Creams

Different types of ice creams available in Dhaka were analyzed for their bacteriological quality. A total number of 24 ice cream samples from two different brands (Brand 1 and 2) were collected from street vendors and local shops of different parts of Dhaka city. Average total viable count in the Brand 1 samples ranged from 9.5x102 to 4.72 x104 cfu/ml, while total coliform count and fecal coliform counts were 2.3x101 to 2.43x103 cfu/ml and zero to 100 cfu/ml, respectively. In case of Brand 2, average total viable count, total coliform count and fecal coliform count were 9.5x103 to 4.43x104 cfu/ml; 10 to 2.49x103 cfu/ml and zero to 200 cfu/ml, respectively. Bacteria isolated from the ice cream samples included Staphylococcus sp. (96%), Bacillus sp (50%), Listeria sp. (41.66%), Micrococcus sp. (12.5%), Escherichia coli (8.33%) and Klebsiella sp (8.33%). Presence of these bacteria in the ice cream indicates the lack of good manufacturing practice in the production of ice cream, which imposes great risk to the consumers.Bangladesh J Microbiol, Volume 29, Number 1, June 2012, pp 41-43

scholarly journals Microbiological Quality and Adulteration of Pasteurized and Raw Milk Marketed in Dharan, Nepal

2020 ◽  
pp. 37-44
Author(s):  
Dhiren Subba Limbu ◽  
Kamana Bantawa ◽  
Dil Kumar Limbu ◽  
Mandira Devkota ◽  
Monika Ghimire
Keyword(s):  
Microbiological Quality ◽  
Raw Milk ◽  
Total Coliform ◽  
Viable Count ◽  
Total Viable Count ◽  
Pasteurized Milk ◽  
Coliform Count ◽  
Standard Guideline ◽  
Routine Monitoring

 This study was aimed to evaluate the quality of raw and pasteurized milk marketed in Dharan. Milk may be contaminated with pathogenic microorganisms and a mixture of several adulterants and such milk pose a risk to consumers. The study was carried out from September 2019 to January 2020. Collected samples were tested for adulterants (starch, formalin, neutralizer and table sugar) as well as microbial quality (Total Coliform count, Total Viable Count, Thermoduric Count, Escherichia. coli and Staphylococcus aureus) as per standard guideline. The adulterants starch, formalin and neutralizer were not detected in both raw and pasteurized milk. However, table sugar was present in 45% (9 out of 20) raw milk and 90% (18 out of 20) pasteurized milk. The average Total Viable Count, Total Coliform Count and Thermoduric Count of raw milk were, 59×105 CFU/ml, 14×104 CFU/ml and 5×103 CFU/ml respectively. Similarly, the average Total Viable Count, Total Coliform Count and Thermoduric Count of pasteurized milk were found to be 15×104 CFU/ml, 14×103CFU/ml and 4×103 CFU/ml respectively. E. coli was detected in 30% pasteurized milk whereas S. aureus was isolated from only 20%. Likewise, E.coli and S. aureus were found in 55% and 45% of raw milk respectively. The results of the study indicated that routine monitoring of dairy industries and raw milk vendors, awareness campaign and good hygienic practice should be promoted to upgrade the quality of raw and pasteurized milk.

scholarly journals Microbial Status and Multidrug Resistance Pattern of Pathogenic Bacteria Isolated from Street Food in Dhaka City, Bangladesh

2018 ◽  
pp. 1-13
Author(s):  
Avijit Banik ◽  
Maruf Abony ◽  
Suvamoy Datta ◽  
Syeda Tasneem Towhid
Keyword(s):  
Pathogenic Bacteria ◽  
Total Coliform ◽  
Viable Count ◽  
Plate Count ◽  
Diffusion Method ◽  
Dhaka City ◽  
Total Viable Count ◽  
Microbiology Laboratory ◽  
Street Food ◽  
Coliform Count

Aims: This study aims to evaluate the microbiological safety in street foods available in Dhaka city, Bangladesh. Study Design: Seven categories of street foods were collected aseptically in triplicates from 10 locations of the most populous areas of Dhaka city, transported to the microbiology laboratory of Center of Excellence, Primeasia University, Dhaka. The samples were analyzed for microbiological quality. Place and Duration of Study: The study was carried out in Dhaka city, Bangladesh, between November 2015 to March 2017. The microbiological analysis was done at the microbiology laboratory of the Center for Excellence Laboratory (CEL), Department of Microbiology, Primeasia University, Dhaka-1213, Bangladesh. Methodology: Ninety street food samples belonging to 7 different categories were collected aseptically from ten different places in Dhaka. All samples were tested according to the standard food analysis methods. Total viable count (TVC), total coliform count (TCC), total Salmonella-Shigella count (TSSC) and total S. aureus count (TSAC) were estimated by using Plate Count agar (PCA), MacConkey agar, Salmonella-Shigella agar and Mannitol Salt agar plates respectively. Kirby-Bauer disc diffusion method on Mueller Hinton agar was used to determine the sensitivity of the isolated strains to commonly prescribed antibiotics. Results: Fried Aubergine, sugarcane juice, potato balls, peanut, rice cake, sweetened coconut, local salty snacks nimki and chanachur, sesame cookies revealed high total viable count (1011 CFU/gm or /mL) and high total coliform count (109 CFU/g or /mL). Some street foods were found to contain potential pathogens such as Escherichia coli, Staphylococcus aureus, Salmonella spp., Vibrio spp. and Campylobacter spp. Some of the isolates of E. coli, S. aureus and Salmonella isolates were found to be resistant against azithromycin (15 µg), sulphomethoxazole (25 μg), penicillin (10 µg), nalidixic acid (30 µg), vancomycin (30 µg) and tetracycline (30 μg). Conclusion: This study reveals the presence of pathogenic bacteria in street foods of Dhaka, Bangladesh. Hence, there is a necessity for strict surveillance on microbial safety of street foods. There should be public engagement projects for.public awareness against consumption of low-quality and unhygienic street foods of Dhaka, Bangladesh.

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