Evaluation of steam-ultrasound decontamination on naturally contaminated broilers through the analysis of Campylobacter, total viable count, and Enterobacteriaceae

2021 ◽  
Author(s):  
hanieh musavian ◽  
Tariq M. Butt ◽  
Aaron Ormond ◽  
David Keeble ◽  
Niels H. Krebs
Keyword(s):  
Viable Count ◽  
Ultrasound Treatment ◽  
Total Viable Count ◽  
Continuous Processing ◽  
Log Reduction ◽  
Surface Areas ◽  
Ultrasound Application ◽  
Poultry Processing ◽  
Microbial Analysis ◽  
Skin Samples

Combined steam-ultrasound process was investigated for decontamination of freshly slaughtered broilers. Combined steam-ultrasound was delivered simultaneously through specially designed nozzles. The nozzles were installed inside of a constructed machine that allowed for continuous processing. The aim of this study was to evaluate the decontamination effect of the steam- ultrasound application with a capacity of 10,500 birds/hour on naturally contaminated broilers, using three different skin sampling areas for microbial analysis (back, breast and the neck skin). Microbial analysis of Campylobacter , Enterobacteriaceae and Total Viable Count (TVC), was performed pre- and -post steam-ultrasound treatment. A total of 648 skin samples were analyzed for Campylobacter and a total of 216 samples were analyzed for Enterobacteriaceae and TVC. R esults showed significant (p<0.001) Campylobacter reductions of 0.8 log, 1.1 log and 0.7 log, analyzed from skin samples taken from the back, breast and the neck, respectively. Significant reductions of Enterobacteriaceae (p<0.001) by 1.6 log, 1.9 log and 1.1 log and significant reductions of TVC (p<0.001) by 2.0 log, 2.4 log and 1.3 log were found on back, breasts and neck, respectively. Refrigeration effect on Campylobacter numbers pre- and post- steam-ultrasound treatment and incubation at 4°C for eight days was determined in a small trial with 12 samples. Results showed significant (p<0.01) reductions of 0.9 log analyzed on breast skin samples, and 0.7 log reduction (p<0.05) on neck skin samples. Results in this study showed that significant bacteria reduction was achieved on three different surface areas on broilers at a slaughter-speed of 10,500b/h with temperatures over 80°C. The rapid treatment of less than 1.5s exposure time per bird chamber, makes this technology potentially suitable for modern and fast poultry processing lines.

scholarly journals Nutritional Benefit, Acceptability and Safety of Cookies from Blanched Coconut and Wheat Flour

2020 ◽  
pp. 94-102
Author(s):  
Abiodun Omowonuola Adebayo- Oyetoro ◽  
Okereke Jane A. ◽  
Ogunnubi, Ebunoluwa I. ◽  
Ukwenya, Anita A. ◽  
Ogundipe, Flora O. ◽  
...  
Keyword(s):  
Wheat Flour ◽  
Calorific Value ◽  
Viable Count ◽  
Total Viable Count ◽  
Crude Fibre ◽  
Standard Methods ◽  
Sensory Score ◽  
Microbial Analysis ◽  
Level Of Significance ◽  
Flour Blends

Cookies were made from wheat and blanched coconut flour blends in ratios 90:10, 85:15, 80:20, 75:25, 70:30, 60:40 and 55:45 respectively with 100% wheat as control. Proximate, sensory and microbial analysis of the cookies were assessed using standard methods and established level of significance difference (p<0.5). Results showed that moisture, crude fibre, fat and calorific value ranged between 5.72%-7.80%, 5.77%-7.80%, 9.81-17.21% and 197.08-312.85 Kcal/100g respectively showing increased parameters with increased substitution levels. Carbohydrate and protein ranged from 49.27%-78.21% and 12.69%-15.66% respectively. Mean sensory score for colour, crunchiness and flavor ranged between 7.30-8.50, 7.01-8.02 and 6.30-8.50 respectively while sample 70:30 was the most acceptable in most of the parameters assessed. Total viable count ranged from 1.4x104 cfu/g- 2.5x104 cfu/g between 2nd -5th week of storage while most samples had no fungi growth for the five weeks except for sample 60:40 and 55:45 with growth ranging from 4.0-7.1x104 Cfu/g. Safe and nutritious cookies can be made from wheat-blanched coconut flour.

scholarly journals Microbial analysis of locally prepared vended dishes in owerri municipality of imo state, Nigeria

2020 ◽  
Vol 3 (10) ◽  
pp. 239-243
Author(s):  
Mercy Adaramaja. F
Keyword(s):  
Total Coliform ◽  
Food Samples ◽  
Viable Count ◽  
Gas Production ◽  
Total Viable Count ◽  
Mould Growth ◽  
White Rice ◽  
Imo State ◽  
Microbial Analysis ◽  
High Sense

A study was carried out to access the microbial analysis of locally prepared vended dishes in Owerri municipality of Imo state. Five zones namely: Prefab and Aladinma areas are represented as Zone A while Ikenegbu and Wethdral areas, Douglas area, School road and Tetlow areas and Worldbank area represented Zone B, C, D and E respectively. The food samples collected includes; rice, spagehetti, beans and white stew. The result obtained revealed that the total viable count in zone A contained 11.42 × 103, 9.5 × 103, 4.4 × 104 and 10.0 × 105 (Cfu/mL) for white rice, spaghetti, beans and stew while those of zone B are 9.0× 103, 7.70 × 103, 2.5× 104 and 6.0 × 105 (Cfu/mL) for white rice, spaghetti, beans and stew respectively. Zone C stew (10.4 ×103 cfu/mL), spaghetti (2.0× 104 cfu/mL), beans (3.3× 104 cfu/mL) and stew (10.3× 104 cfu/mL). Zone D stew (8.0 × 103 cfu/mL), spaghetti (1.5× 104 cfu/mL), beans (1.4× 104 cfu/mL) and stew (4.0×105cfu/mL). Zone E stew (12.55 × 103 cfu/mL), spaghetti (6.3× 103 cfu/mL), beans (6.0× 104 cfu/mL) and stew (4.5×105cfu/mL). Total coliform count shows no gas production in Durbam tube in all the zones, no mould were also observed in all the samples. Generally, mould growth on food is evidence deterioration. It was concluded that the zones maintained high sense of hygiene.

Investigation of microbial contamination of Powdered Infant Formula during different storage periods after opening

2019 ◽  
Vol 10 (03) ◽  
Author(s):  
Mohammed Khalid Al-atrash
Keyword(s):  
Infant Formula ◽  
Environmental Protection Agency ◽  
Microbial Contamination ◽  
Total Coliform ◽  
Viable Count ◽  
Risk Level ◽  
Powdered Infant Formula ◽  
Total Viable Count ◽  
Coliform Count ◽  
Significant Difference

The present study was carried out to knowing effect different storage periods of the microbial quality for the Powdered Infant Formula (PIF) after opening the tin, and ensuring from the safety note (after opening, use within 3 weeks). Thirty (30) samples of (PIF) from category (1 – 6 months) in five different types are collected from pharmacies and local markets in Baquba city / Iraq, which are used as substitutes for breast milk during the first day of opening the tin powders such as Total viable count, Total coliform count, Salmonella count and Yeast and Molds count. These experiments repeated at each week of same samples within (5) weeks. Results were obtained at opening the tin, Total viable count (less than 0.05 1.0 x 103 ±1.5x10 CFU/g) were significantly higher than Total Coliform count (less than 0.05 ± 0.3 x 10 CFU/g) and Total Salmonella count (less than 0.05 0 x 10 CFU/g) and Yeasts and Molds (less than 0.05 ± 0.3 x 10 CFU/g). while results obtained at fifth week were (less than 0.05 8.8 x 103 ±5.5x102 CFU/g), (less than 0.05 0.9 x 102 ± 0.4x101 CFU/g), (less than 0.05 0 x 10 CFU/g), (less than 0.05 9.5 x 10 ± 1.2x101 CFU /g) respectively. All samples of (PIF) having non-significant difference. These results compared to Iraqi Quality Standards (IQS), all the results from the opening samples to fifth week were within the range of IQS and USA Environmental Protection Agency (USEPA) and as indicates the hygienic condition of (PIF) without risk level for human health. also observed increase in microbial contamination in each week because increase the moisture content for powdered milk. Can be used more than 3 weeks after opening if stored in good conditions with good hygienic practices during milk preparation.

scholarly journals Evaluation of non-thermal effect of microwave radiation and its mode of action in bacterial cell inactivation

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Priyanka Shaw ◽  
Naresh Kumar ◽  
Sohail Mumtaz ◽  
Jun Sup Lim ◽  
Jung Hyun Jang ◽  
...  
Keyword(s):  
Thermal Effect ◽  
Microwave Radiation ◽  
Bacterial Cell ◽  
Mode Of Action ◽  
Surface Damage ◽  
Peak Frequency ◽  
Viable Count ◽  
Bacterial Inactivation ◽  
Bacterial Strains ◽  
Log Reduction

AbstractA growing body of literature has recognized the non-thermal effect of pulsed microwave radiation (PMR) on bacterial systems. However, its mode of action in deactivating bacteria has not yet been extensively investigated. Nevertheless, it is highly important to advance the applications of PMR from simple to complex biological systems. In this study, we first optimized the conditions of the PMR device and we assessed the results by simulations, using ANSYS HFSS (High Frequency Structure Simulator) and a 3D particle-in-cell code for the electron behavior, to provide a better overview of the bacterial cell exposure to microwave radiation. To determine the sensitivity of PMR, Escherichia coli and Staphylococcus aureus cultures were exposed to PMR (pulse duration: 60 ns, peak frequency: 3.5 GHz) with power density of 17 kW/cm2 at the free space of sample position, which would induce electric field of 8.0 kV/cm inside the PBS solution of falcon tube in this experiment at 25 °C. At various discharges (D) of microwaves, the colony forming unit curves were analyzed. The highest ratios of viable count reductions were observed when the doses were increased from 20D to 80D, which resulted in an approximate 6 log reduction in E. coli and 4 log reduction in S. aureus. Moreover, scanning electron microscopy also revealed surface damage in both bacterial strains after PMR exposure. The bacterial inactivation was attributed to the deactivation of oxidation-regulating genes and DNA damage.

Biodegradation Potential of Abattoir Wastewater Microbiota in Nigeria

2020 ◽  
Vol 15 (3) ◽  
pp. 70-77
Author(s):  
J.D Bala ◽  
F. A Kuta ◽  
N.U Adabara ◽  
O.P Abioye ◽  
H.S Auta ◽  
...  
Keyword(s):  
Physicochemical Parameters ◽  
Oxygen Demand ◽  
Total Suspended Solid ◽  
Sole Source ◽  
Suspended Solid ◽  
Viable Count ◽  
Total Viable Count ◽  
Slaughter House ◽  
Abattoir Wastewater ◽  
Total Dissolved Solid

Water used for washing carcasses of slaughtered animals and slaughter house is referred to as abattoir wastewater. This study was designed to investigate the microorganisms associated with abattoir wastewater and to establish the biodegradation potential of abattoir wastewater microbiota. Isolation of the microbes was carried out using pour plate technique. The total viable count for the microbes’ ranges from 2.5×104 - 4.6×105 cfu/mL. Results revealed that all the physicochemical parameters exceeded the permissible limits (total dissolved solid (TDS) 1748mg/L, total suspended solid (TSS) 176mg/L, biochemical oxygen demand (BOD5) 91 mg/L and chemical oxygen demand (COD) 227 mg/L). Microorganisms isolated include Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis, Bacillus anthracis, Aspergillus niger, A. flavus, Mucor sp, Trichophyton quickeanum and Penicillium sp. Some of the microbes were observed to have biodegradation potential by their ability to grow on mineral salt media (MSM) incorporated with starch, cellulose, crude oil, kerosene and diesel as the sole source of carbon and energy. This study suggests that abattoir wastewater harbors microorganisms that could be hazardous to public health when discharged into the environment untreated hence the need for strict monitoring. These microbes isolated could be employed as agent of bioremediation of wastewaters. Key words: Abattoir; Biodegredation; Isolation; Microbiota; Wastewater

scholarly journals Quality of ultra-high temperature treated milk available in Gazipur and Mymensingh of Bangladesh

2016 ◽  
Vol 44 (3) ◽  
pp. 132-136
Author(s):  
MY Arafat ◽  
R Habib ◽  
MSR Siddiki ◽  
MF Imam
Keyword(s):  
High Temperature ◽  
Specific Gravity ◽  
Viable Count ◽  
Total Viable Count ◽  
Total Solids ◽  
Uht Milk ◽  
Coliform Count ◽  
Milk Samples ◽  
Ultra High Temperature

This experiment was conducted to evaluate the quality of existing ultra-high temperature (UHT) treated milk available in two selected town markets of Bangladesh. A total of 27 UHT milk samples from three different brands–Pran Dairy (A), Aarong Dairy (B) and Farm Fresh (C) were collected from local markets at Mymensingh and Gazipur districts during the period of 1stSeptember to 29th November, 2014. Parameters studied in this experiment were organoleptic (flavour, consistency, colour and appearance), physic-chemical (specific gravity, acidity, total solids, solids-not-fat, fat, protein, lactose, and ash) and microbiological (Total Viable Count and Coliform Count). All the samples were similar in respect of color, flavor, taste and texture, and no significant difference was observed. However, significant differences were revealed (p<0.05) in case of specific gravity, acidity, total solids, solids-not-fat, and protein content. On the other hand, insignificant differences were found (p>0.05) in case of fat, lactose, and ash content. Total Viable Count and Coliform count were found to be nil in all the milk samples. Milk samples of Farm Fresh UHT milk were superior to other brands of UHT Milk in terms of contents of total solids (119.23±0.57 g/kg),fat (34.97±0.35g/kg), lactose (43.23±0.51g/kg), and ash (7.00±0.26g/kg). Though there were some fluctuations in all the parameters studied, all the milk samples conformed to the standard values for UHT milk, and in general all of the UHT milk samples studied in this experiment was of good quality.Bang. J. Anim. Sci. 2015. 44 (3):132-136

ATP bioluminescence rapid detection of total viable count in soy sauce

Luminescence ◽  
2011 ◽  
Vol 27 (1) ◽  
pp. 34-38 ◽  
Author(s):  
Shou-Lei Yan ◽  
Su-Na Miao ◽  
Shao-Ya Deng ◽  
Min-Juan Zou ◽  
Fo-Sheng Zhong ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Viable Count ◽  
Soy Sauce ◽  
Total Viable Count ◽  
Atp Bioluminescence

scholarly journals Effect of adding essential oils of caraway and rosemary on volatile aroma compounds derived from stored vacuum packaged minced turkey meat

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Magdalena Michalczyk ◽  
Paweł Satora ◽  
Joanna Banaś ◽  
Grzegorz Fiutak
Keyword(s):  
Beneficial Effect ◽  
Essential Oils ◽  
Solid Phase ◽  
Control Sample ◽  
Acid Value ◽  
Fat Oxidation ◽  
Viable Count ◽  
Gas Chromatography Mass Spectrometry ◽  
Total Viable Count ◽  
Turkey Meat

AbstractChanges in the odor of meat during its storage are one of the basic indicators affecting its assessment and possible disqualification. The aim of the study was to determine whether the addition of essential oils may affect the composition and concentration of volatile compounds included in the aroma of stored turkey meat. We investigated the effect of adding essential oil (EO) of caraway (0.02% v/w), rosemary (0.02% v/w) and a mixture of the two (0.01% each) on the composition of volatile compound fractions formed during 10-day storage of vacuum-packed minced turkey meat. The EOs used were also evaluated for their influence on microbial contamination (total viable count and lactic acid bacteria count), sensory quality (odor and taste) and the level of fat oxidation (acid value, peroxide value and p-anisidine value) and pH in chill-stored samples. In terms of sensory indicators, the greatest beneficial effect of adding oils was noted in the odor of raw meat. Use of the HS-SPME/GC-MS (headspace-solid phase microextraction/gas chromatography-mass spectrometry) method showed that the addition of oils significantly reduced the amounts of benzeneacetaldehyde, 2-octenal and ethyl 2-methyloctanoate compared with the control sample. In addition, in the presence of essential oils of rosemary and caraway, decreases were noted in benzaldehyde and 9-octadecenal, respectively. These changes may potentially affect the reception of the odor. There was a beneficial effect of the oils in reducing the levels of fat oxidation indicators, including peroxide. However, the oils at applied concentrations had no significant effect on the total viable count and LAB count.

scholarly journals Effect of Antibacterial Agents on the Microbial Flora of Some Fruits and Vegetables.

2020 ◽  
pp. 299-304
Author(s):  
Omorodion Nnenna ◽  
Oriji Gift
Keyword(s):  
Fruits And Vegetables ◽  
Antimicrobial Agents ◽  
Reduction Rate ◽  
Total Coliform ◽  
Viable Count ◽  
Microbiological Analysis ◽  
Total Viable Count ◽  
Coliform Count ◽  
Microbiological Methods ◽  
Bacillus Spp

Six samples of fruits (apple, banana, mango, orange, pineapple and watermelon) and six samples of vegetables (cabbage, carrot, cucumber, lettuce, potato and tomato) were purchased from wokem market in Choba town PH, Rivers State and microbiological analysis were carried out these fruits and vegetables samples and the effects of the antimicrobial agents (Ethanol and Hydrogen peroxide) on the microbial load. The Total viable count, Total coliform count and Escherichia count were determined via standard microbiological methods. The Total viable count ranged from 4.1x10106 to 1.0x107 cfu/g Total coliform count from 4.5x107 to 1.5x106 cfu/g nd the E.coli count ranged from 5.1x102 to 1.0x102 cfu/g. The Total viable count for apple ranged between to 5.1 x107 to 2.07x109 , Cabbage 1.45x109 to 4.9x107 , banana 1.10x109 to 5.9x107 , cucumber 1.82x108 to 6.0x107. The microorganisms isolated were Escherichia coli 42%, Staphylococcus aureus 30%, Pseudomonas spp 18%, Bacillus spp,6% and Serratia spp, 2%. Hydrogen perioxide had a high pathogen reduction rate than ethanol.The isolation of E coli and other pathogenic microorganisms indicates the presences of faecal contamination and the poor hygienic production, storage and transport system. Fruits and vegetables should be properly handled from their seeds planted in the farm to the matured ones sold in the market

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