scholarly journals Effect Concentration of Moringa (Moringa oleifera Lam) Leaf Extract in Citrate-Egg Yolk in Maintaining Motility and Viability of Spermatozoa of Kacang Goat

2021 ◽  
Vol 16 (4) ◽  
pp. 340-346
Author(s):  
R. R. Dapawole ◽  
I. P. Sirappa

This study aimed to determine the effectiveness and the best concentration of Moringa leaf extract (MLE) in the citrate-egg yolk (C-EY) to maintain the motility and viability of spermatozoa kacang goat. Semen was collected from 3 goats aged two years; by using the artificial vagina method. The semen was evaluated macroscopically and microscopically. The semen that had >70% sperm motility and >250x106/ml sperm concentration was divided into 4 equal tubes, each diluted with100% C-EY (P1), 10% MLE+ 90%C-EY (P2), 20% MLE +80% C-EY (P3), and 30% MLE+70% C-EY (P4). The diluted samples were then stored in a refrigerator (3-5?C) and evaluated for motility and viability every 24 hours. The study was designed using a completely randomized design (CRD) consisting of four treatments and five replications. The results showed that the addition of MLE in C-EY significantly affected goat spermatozoa's progressive motility and viability. The data showed that the spermatozoa kept during four days in a diluent of P2 had higher (P<0.05) motility 44.67±4.80% and viability 74.24±4.46%than the other three diluents of P1(36.00±4.70%; 70.10±3.6%), P3(33.67±0.42%; 66.85±4.99%) and P4 (29.67±3.99%; 63.96±5.44%). This study concluded that adding 10% MLE was the best concentration as source energy in 90% C-EY diluents, which effectively maintained the motility and viability of kacang goat spermatozoa for four days of storage at a temperature of 3-5oC.

Author(s):  
A. J. Gomes Filho ◽  
S. C. de Paiva ◽  
G. M. C. Takaki ◽  
A. S. Messias

This study aims to analyze the reject produced by the desalinators implanted in the semi-arid Pernambuco in contact with the seeds of Moringa oleifera Lam. For this, Moringa seeds were collected and prepared with the following treatments: reject (control), reject with whole seed with and without husks, reject with crushed seed with and without husks, seed residue with and without husks, in five replicas. Each replica was constituted with doses equivalent to 2.0 g of Moringa seeds for 200 mL of reject, with contact time corresponding to 30, 60, 120 and 180 minutes, in a completely randomized design, under laboratory conditions, total of 140 experimental units. The physical-chemical and statistical analyses were performed through analysis of variance (ANOVA), and the F test, at confidence interval of 95%. It has been found through laboratory tests that seeds with crushed or ground Moringa husks are equally effective at adsorbing sodium from 1,868,0 mg/L to 24,6 mg/L (98,7 %), calcium from 1,005.0 mg/L to 894.6 mg/L (11%), magnesium from 741.0 mg/L to 728.3 mg/L (1.7%) and chloride from 6,997.5 mg/L to 6,782.4 mg/L (1.8%). Therefore, Moringa seed can be considered promising in the adsorption of chemical elements of saline water desalinizers.


2020 ◽  
Vol 21 (supplement 1) ◽  
Author(s):  
Jawad Kadhim Obaid Al-Hujayri ◽  
Abdoun H. Alwan ◽  
Neepal Imtair Al-garaawi

The effect of different levels of nano- and mineral-Iron and their method of application on anatomical properties of the upper and lower epidermis of Moringa (Moringa oleifera Lam.) leaves was studied. Seeds of Moringa were sown in 15 kgs soil capacity pots during the growing season of 2018/2019 arranged as a factorial experiment within Completely Randomized Design, with three replicates. The factorswere a method of fertilization(soil or foliar application),three levels i.e. 0,180 and 360mg.l-1 of each of mineral and nano-Iron. The total number of experimental units was 2×3×3×3 for method, nano-Iron, mineral-Iron, and replicates respectively.The application method caused an increase in the mean dimensions of normal epidermis cells, stomatoes, and hairs.These traits were increased as Iron levels increased. Where 360 mg.l-1 of both types of Iron gave the highest dimensions. Soil application gave higher values of epidermis dimensions than that obtained from the foliar application. The number of stomatoesincreased with increasing the level of Iron. The number of normal epidermis cells was conversely proportioned with their volume.


AQUASAINS ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 769
Author(s):  
Febri Setyawati ◽  
Kismiyati Kismiyati ◽  
Sri Subekti

Abstrak Goldfish (Carassius auratus) is one of the fishes that can be infected by parasites. Commonly, this fish is often attacked by Argulus japonicus ectoparasites. Control of infestation in A. japonicus can be done by utilizing one of the natural ingredients, Moringa oleifera leaf extract, which contains of alkaloids, tannins, saponins and flavonoids. This study aims to determine the effect of M. oleifera leaf extract in reducing the infestation of A. japonicus and the optimal concentration of M. oleifera leaf extract in reducing the infestation of A. japonicus in goldfish (C. auratus). This study used a completely randomized design (CRD) with treatments namely control, 600 ppm, 700 ppm, 800 ppm and 900 ppm with four times of replication. The main parameter is the percentage decrease in infestation of A. japonicus. The results of the research data were analyzed by using ANOVA and followed with the Ducan Multiple Range Test. The results showed that Moringa leaf extract could reduce the infestation of A. japonicus. The optimal concentration of Moringa leaf extract in reducing the infestation of A. japonicus was obtained from a concentration of 700 ppm.


2018 ◽  
Vol 4 (1) ◽  
Author(s):  
Sawaludin Sawaludin ◽  
Aluh Nikmatullah ◽  
Bambang Budi Santoso

This study aimedseedling derived from stem cutting to determine the effect of various media on the growth of drum stick (Moringa oleifera Lam.). Experiment was conducted from March to May 2017 in the nursery located in Dasan Agung, Mataram, with altitude of 16 meters above sea level. The method was experimental method with 6 media treatments: soil, soil+sawdust, soil+cocopeat, soil+corncob, soil+bamboo leaf, and soil+charcoal rice husk. The experimental design was Completely Randomized Design with 5 replications and 5 unit series. The result shows that the media significantly effect on the growth ofsedling stem cutting. The best media forseedling derived from stem cutting were mixture media of soil+ricecharcoal andsoil+cocopeat


2018 ◽  
Vol 4 (2) ◽  
pp. 132
Author(s):  
Rasmani Rasmani ◽  
I Gusti Made Arya Parwata ◽  
Bambang Budi Santoso

The experimental research is aimed to find out the kind of nursery organic media for better growth of drumstick seedling. It was implemented in January-April 2017 in the nursery field in Dasan Agung, Mataram. The treatments studied were kinds of nursery media including soil media, mixture of soil-sawdust, mixture of soil-cocopeat, mixture of soil-crushed corncobs, mixture of soil-bamboo leaf litter. The experiment design was arranged in a Completely Randomized Design with 5 replications and each content of 5 serial experiment units. The result shows that the kind of seedling media significantly affected the growth to Moringa seedling growth. The soil-cocopeat mixture medium, soil-crushed corn cobs, and soil-bamboo leaf litter were the best medium and more suitable for drumstick seedling compared to soil media and soil-sawdust mixture media


2018 ◽  
Vol 16 (1) ◽  
pp. 23-34
Author(s):  
F Islam ◽  
MA Hossain ◽  
MF Rahman ◽  
MA Hashem ◽  
M Rahman ◽  
...  

The experiment was conducted to compare the effect of Moringa oleifera leaf extract with synthetic antioxidant on beef meatball. Five types of beef meatballs were formulated for this purpose. Meatballs were made with control (0%), 0.1% Beta Hydroxyl Anisole (BHA), 0.1, 0.2 and 0.3% Maringa oleifera leaf extract, respectively. Quality and safety evaluation of meatballs were determined by sensory, physiochemical, biochemical and microbiological tests. After preparation meatballs were preserved at -20˚C. The analyses were conducted at 0, 15t, 30 and 60 days of interval. An ANOVA of a 5x4 factorial experiment in completely randomized design having three replications per treatment was used for the analyses of data. Considering CP, tenderness, juiciness, overall acceptability, cooking loss, FFA, POV and TBARS value it can be concluded that Moringa oleifera leaf extract up to a level of 0.3% may replace BHA for meatball preservation without deteriorating its quality. In case of sensory evaluation 0.2% Moringa leaf extract shows better results. But on the basis of nutrient quality, physicochemical properties, biochemical analysis and microbial analysis 0.3% Moringa leaf extract group is more satisfactory than other treatment groups.SAARC J. Agri., 16(1): 23-34 (2018)


2020 ◽  
Vol 4 (2) ◽  
pp. 216
Author(s):  
Nur Fahlia

The application of moringa leaves flour is finite. This research aims to analyze the substitution effect of Moringa leaves flour on snack bars. This experimental research design uses a Completely Randomized Design (CRD) with three substitution levels including F1 (95% wheat flour and 5% Moringa leaves flour), F2 (90% wheat flour and 10% Moringa leaves flour) and F3 (85% wheat flour and 15% moringa leaves flour). Data from organoleptic test results were analyzed using the ANOVA test if there are significant differences followed by Duncan's further tests. While the results of proximate levels and calcium levels were analyzed using an independent t-test. The organoleptic analysis of the hedonic test showed that the selected snack bar formula was F2. The result of proximate analysis of selected snack bars contains 12.52% water, 1.65% ash, 19.61% fat, 9.23% protein, 56.99% carbohydrate, and 344.14 mg /100g calcium. The contribution of selected snack bar energy is 441.37 kcal per 100 grams and 203.03 kcal per serving size (46 gram). This research concludes that proximate levels in the form of water, ash, fat, protein, carbohydrate have significant differences (p <0.05) in each analysis.  Selected calcium snack bar levels have high calcium claims.      


DEPIK ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 86-90
Author(s):  
Iko Imelda Arisa ◽  
Cut Mutia ◽  
Sri Agustina ◽  
Nurfadillah Nurfadillah ◽  
Sofyatuddin Karina

This study aimed to determine the optimal concentration of Moringa oleifera leaf extract as an antibacterial agent on Litopenaeus vannamei larvae that are infected by Vibrio sp.. It was conducted in 15 March - 15 April 2019 at PT. Global Gain Superior Seeds, Pantai Cermin, North Sumatera. The research used a completely randomized design (CRD) method with five treatments and four replications. The shrimps infected by Vibrio sp. were immersed into M. oleifera leaf extract in various concentration of (A) 0 ppm, (B) 400 ppm, (C) 600 ppm, (D) 800 ppm, and (E) 1000 ppm for ±15 minutes. The size of shrimp used was post-larvae 8 (length of 3.5-5 mm/ind). The best performance results of vannamei shrimps were obtained in treatment E (1000 ppm), namely length growth of 16.75 ± 1.16 cm, weight growth of 0.92 ± 0.89 g and survival rate of 91±7.87%.Keywords:Moringa oleiferaVibrio sp.Immersion


2021 ◽  
Vol 2 (3) ◽  
pp. 192-195
Author(s):  
Anggraeni ◽  
Ristika Handarini ◽  
Deden Sudrajat ◽  
Burhanudin Malik ◽  
Viona Oktavia

Duck egg is known to have an unpleasant fishy odor which often becomes a factor reducing its acceptance in public.  This odor is resulted from lipid oxidation which can be avoided by using an antioxidant.  This study was aimed at assessing the effects of the inclusion of star gooseberry (Sauropus androgynus) dried leaf extract in ration on egg organoleptic quality of local duck.  The study was conducted in a completely randomized design with 4 treatments and 4 replicates.  Sensory test by 60 semi-trained panelists and hedonic quality and hedonic tests were conducted.  Data were subjected to a Kruskal-Wallis test.  Results showed that treatments gave significant effects (P <0.05) on aroma (hedonic), yolk color, and aroma (hedonic quality).  It was concluded that the inclusion of star gooseberry dried leaf extract by 1,5% in ration improved the quality of egg yolk color, egg aroma, and consumers’ preference.  A further study on the effects of DSGLE on meat quality and protein digestibility in ducks was recommended.


2020 ◽  
Vol 7 (2) ◽  
pp. 85-89
Author(s):  
Isye Jean Liur

Hen egg is easy to get and affordable. Besides having a complete nutritional content, chicken eggs are also easily damaged due to microbial activity during storage so that it can reduce egg quality. Therefore, a preservation process is needed to maintain egg quality. This study aims to determine the effect of soursop leaf extract concentrations on decreasing the quality of eggs. This research used a completely randomized design with two factors, the first factor was the concentration of soursop leaf extract (20, 30, 40%) and the second was the shelf life (0, 7, 14, 21 and 28 days). Observation included egg white index, egg yolk index, haugh unit, and weights of eggs. The results indicate that eggs with soursop leaf extract immersion treatment can maintain egg quality. The concentration of soursop leaf extract as much as 40% gives better results in maintaining the quality of eggs.


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