ANATOMICAL STUDY OF MORINGA LEAF (MORINGA OLEIFERA LAM.) UNDER IRON FERTILIZATION

The effect of different levels of nano- and mineral-Iron and their method of application on anatomical properties of the upper and lower epidermis of Moringa (Moringa oleifera Lam.) leaves was studied. Seeds of Moringa were sown in 15 kgs soil capacity pots during the growing season of 2018/2019 arranged as a factorial experiment within Completely Randomized Design, with three replicates. The factorswere a method of fertilization(soil or foliar application),three levels i.e. 0,180 and 360mg.l-1 of each of mineral and nano-Iron. The total number of experimental units was 2×3×3×3 for method, nano-Iron, mineral-Iron, and replicates respectively.The application method caused an increase in the mean dimensions of normal epidermis cells, stomatoes, and hairs.These traits were increased as Iron levels increased. Where 360 mg.l-1 of both types of Iron gave the highest dimensions. Soil application gave higher values of epidermis dimensions than that obtained from the foliar application. The number of stomatoesincreased with increasing the level of Iron. The number of normal epidermis cells was conversely proportioned with their volume.

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Application of Moringa in the Removal of Salts from the Desalinator Reject

Current Journal of Applied Science and Technology ◽

10.9734/cjast/2019/v36i130212 ◽

2019 ◽

pp. 1-13

Author(s):

A. J. Gomes Filho ◽

S. C. de Paiva ◽

G. M. C. Takaki ◽

A. S. Messias

Keyword(s):

Contact Time ◽

Moringa Oleifera ◽

Chemical Elements ◽

Saline Water ◽

Physical Chemical ◽

Randomized Design ◽

Completely Randomized Design ◽

Whole Seed ◽

Semi Arid ◽

Moringa Oleifera Lam

This study aims to analyze the reject produced by the desalinators implanted in the semi-arid Pernambuco in contact with the seeds of Moringa oleifera Lam. For this, Moringa seeds were collected and prepared with the following treatments: reject (control), reject with whole seed with and without husks, reject with crushed seed with and without husks, seed residue with and without husks, in five replicas. Each replica was constituted with doses equivalent to 2.0 g of Moringa seeds for 200 mL of reject, with contact time corresponding to 30, 60, 120 and 180 minutes, in a completely randomized design, under laboratory conditions, total of 140 experimental units. The physical-chemical and statistical analyses were performed through analysis of variance (ANOVA), and the F test, at confidence interval of 95%. It has been found through laboratory tests that seeds with crushed or ground Moringa husks are equally effective at adsorbing sodium from 1,868,0 mg/L to 24,6 mg/L (98,7 %), calcium from 1,005.0 mg/L to 894.6 mg/L (11%), magnesium from 741.0 mg/L to 728.3 mg/L (1.7%) and chloride from 6,997.5 mg/L to 6,782.4 mg/L (1.8%). Therefore, Moringa seed can be considered promising in the adsorption of chemical elements of saline water desalinizers.

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The Effect of Using Different Levels of Thinner Olive Oil (Extra Virgin Olive Oil) on the Viability and Abnormality of Spermatozoa and pH of the Semen Duroc Pigs

Journal of Tropical Animal Science and Technology ◽

10.32938/jtast.v3i2.1024 ◽

2021 ◽

Vol 3 (2) ◽

pp. 55-66

Author(s):

Stefanus Nahak ◽

Agustinus Agung Dethan ◽

Paulus Klau Tahuk

Keyword(s):

Olive Oil ◽

Ph Value ◽

Virgin Olive Oil ◽

Extra Virgin Olive Oil ◽

North Central ◽

Randomized Design ◽

The Mean ◽

Completely Randomized Design ◽

Different Levels

This study aims to determine the effect of using different levels of thinner olive oil (extra virgin olive oil) on the viability and abnormality of spermatozoa and the pH of the semen of duroc pigs. The research took place in September 2020 in Noenebu, Tapenpah Village, Insana District, North Central Timor Regency, and the Laboratory of the Faculty of Agriculture, University of Timor. This study used a completely randomized design (CRD) with 4 treatments and 5 replications. Treatment R1 consist of 10% tris solution, 10% olive oil, 80% semen; R2 treatment consist of 15% tris solution, 15% olive oil, 70% semen; R3 treatment consist of 20% tris solution, 20% olive oil, 60% semen, and R4 treatment consist of 25% tris solution, 25% olive oil, 50% semen. The results showed that the effect of different treatments was very significant (P <0.01) on the viability of spermatozoa. The mean spermatozoa viability of each treatment was R1 of 89 ± 2.57%, R2 of 76.9 ± 12.51%, R3 of 62.8 ± 3.11%, and R4 treatment of 41.2 ± 15, 38%. The spermatozoa abnormalities were significantly different (P <0.05). Where the abnormality of the spermatozoa in treatment R1 was 8.8 ± 1.30%, R2 was 10.4 ± 1.67%, R3 was 11.6 ± 1.81%, and R4 was 14.4 ± 3.78%. The degree of acidity (pH) of the semen was not significantly different between treatments. The pH value of each treatment was R1 of 7.92 ± 0.83, R2 of 8.04 ± 0.54, R3 of 7.96 ± 0.39; and R4 treatment of 8.06 ± 0.64. It can be concluded that the addition of 10% to 20% olive oil can maintain the viability and abnormality of duroc boar male spermatozoa. However, the use of olive oil up to 20% does not affect to degree of acidity of the semen produced.

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Pengaruh Berbagai Macam Media terhadap Pertumbuhan Bibit Kelor (Moringa oleifera Lam.) Asal Stek Batang

Jurnal Sains Teknologi & Lingkungan ◽

10.29303/jstl.v4i1.65 ◽

2018 ◽

Vol 4 (1) ◽

Author(s):

Sawaludin Sawaludin ◽

Aluh Nikmatullah ◽

Bambang Budi Santoso

Keyword(s):

Experimental Design ◽

Experimental Method ◽

Sea Level ◽

Moringa Oleifera ◽

Stem Cutting ◽

Soil Charcoal ◽

Randomized Design ◽

The Media ◽

Completely Randomized Design ◽

Moringa Oleifera Lam

This study aimedseedling derived from stem cutting to determine the effect of various media on the growth of drum stick (Moringa oleifera Lam.). Experiment was conducted from March to May 2017 in the nursery located in Dasan Agung, Mataram, with altitude of 16 meters above sea level. The method was experimental method with 6 media treatments: soil, soil+sawdust, soil+cocopeat, soil+corncob, soil+bamboo leaf, and soil+charcoal rice husk. The experimental design was Completely Randomized Design with 5 replications and 5 unit series. The result shows that the media significantly effect on the growth ofsedling stem cutting. The best media forseedling derived from stem cutting were mixture media of soil+ricecharcoal andsoil+cocopeat

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Pertumbuhan Bibit Tanaman Kelor (Moringa oleifera Lam.) Pada Beberapa Macam Media Organik dengan Teknik Pembibitan Tidak Langsung

Jurnal Sains Teknologi & Lingkungan ◽

10.29303/jstl.v4i2.80 ◽

2018 ◽

Vol 4 (2) ◽

pp. 132

Author(s):

Rasmani Rasmani ◽

I Gusti Made Arya Parwata ◽

Bambang Budi Santoso

Keyword(s):

Leaf Litter ◽

Experimental Research ◽

Seedling Growth ◽

Moringa Oleifera ◽

Organic Media ◽

Randomized Design ◽

Soil Media ◽

Completely Randomized Design ◽

Nursery Field ◽

Moringa Oleifera Lam

The experimental research is aimed to find out the kind of nursery organic media for better growth of drumstick seedling. It was implemented in January-April 2017 in the nursery field in Dasan Agung, Mataram. The treatments studied were kinds of nursery media including soil media, mixture of soil-sawdust, mixture of soil-cocopeat, mixture of soil-crushed corncobs, mixture of soil-bamboo leaf litter. The experiment design was arranged in a Completely Randomized Design with 5 replications and each content of 5 serial experiment units. The result shows that the kind of seedling media significantly affected the growth to Moringa seedling growth. The soil-cocopeat mixture medium, soil-crushed corn cobs, and soil-bamboo leaf litter were the best medium and more suitable for drumstick seedling compared to soil media and soil-sawdust mixture media

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PENGARUH SUBTITUSI TEPUNG DAUN KELOR (Moringa oleifera Lam.) TERHADAP SIFAT ORGANOLEPTIK DAN KADAR KALSIUM SNACK BAR

Jurnal Gizi dan Pangan Soedirman ◽

10.20884/1.jgipas.2020.4.2.2794 ◽

2020 ◽

Vol 4 (2) ◽

pp. 216

Author(s):

Nur Fahlia

Keyword(s):

Wheat Flour ◽

Moringa Oleifera ◽

Substitution Effect ◽

Proximate Analysis ◽

Test Results ◽

Randomized Design ◽

High Calcium ◽

Completely Randomized Design ◽

Serving Size ◽

Moringa Oleifera Lam

The application of moringa leaves flour is finite. This research aims to analyze the substitution effect of Moringa leaves flour on snack bars. This experimental research design uses a Completely Randomized Design (CRD) with three substitution levels including F1 (95% wheat flour and 5% Moringa leaves flour), F2 (90% wheat flour and 10% Moringa leaves flour) and F3 (85% wheat flour and 15% moringa leaves flour). Data from organoleptic test results were analyzed using the ANOVA test if there are significant differences followed by Duncan's further tests. While the results of proximate levels and calcium levels were analyzed using an independent t-test. The organoleptic analysis of the hedonic test showed that the selected snack bar formula was F2. The result of proximate analysis of selected snack bars contains 12.52% water, 1.65% ash, 19.61% fat, 9.23% protein, 56.99% carbohydrate, and 344.14 mg /100g calcium. The contribution of selected snack bar energy is 441.37 kcal per 100 grams and 203.03 kcal per serving size (46 gram). This research concludes that proximate levels in the form of water, ash, fat, protein, carbohydrate have significant differences (p <0.05) in each analysis. Selected calcium snack bar levels have high calcium claims.

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Effect Concentration of Moringa (Moringa oleifera Lam) Leaf Extract in Citrate-Egg Yolk in Maintaining Motility and Viability of Spermatozoa of Kacang Goat

Jurnal Sain Peternakan Indonesia ◽

10.31186/jspi.id.16.4.340-346 ◽

2021 ◽

Vol 16 (4) ◽

pp. 340-346

Author(s):

R. R. Dapawole ◽

I. P. Sirappa

Keyword(s):

Leaf Extract ◽

Moringa Oleifera ◽

Sperm Concentration ◽

Egg Yolk ◽

Progressive Motility ◽

Randomized Design ◽

Effect Concentration ◽

Completely Randomized Design ◽

Artificial Vagina ◽

Moringa Oleifera Lam

This study aimed to determine the effectiveness and the best concentration of Moringa leaf extract (MLE) in the citrate-egg yolk (C-EY) to maintain the motility and viability of spermatozoa kacang goat. Semen was collected from 3 goats aged two years; by using the artificial vagina method. The semen was evaluated macroscopically and microscopically. The semen that had >70% sperm motility and >250x106/ml sperm concentration was divided into 4 equal tubes, each diluted with100% C-EY (P1), 10% MLE+ 90%C-EY (P2), 20% MLE +80% C-EY (P3), and 30% MLE+70% C-EY (P4). The diluted samples were then stored in a refrigerator (3-5?C) and evaluated for motility and viability every 24 hours. The study was designed using a completely randomized design (CRD) consisting of four treatments and five replications. The results showed that the addition of MLE in C-EY significantly affected goat spermatozoa's progressive motility and viability. The data showed that the spermatozoa kept during four days in a diluent of P2 had higher (P<0.05) motility 44.67±4.80% and viability 74.24±4.46%than the other three diluents of P1(36.00±4.70%; 70.10±3.6%), P3(33.67±0.42%; 66.85±4.99%) and P4 (29.67±3.99%; 63.96±5.44%). This study concluded that adding 10% MLE was the best concentration as source energy in 90% C-EY diluents, which effectively maintained the motility and viability of kacang goat spermatozoa for four days of storage at a temperature of 3-5oC.

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Pengaruh Pemberian Moringa oleifera Terhadap Kuantitas dan Kualitas Semen Sapi Peranakan Ongole

Jurnal Ilmu dan Teknologi Peternakan Tropis ◽

10.33772/jitro.v7i2.10886 ◽

2020 ◽

Vol 7 (2) ◽

pp. 119

Author(s):

Lukman Affandhy ◽

Muchamad Luthfi ◽

Dian Ratnawati ◽

Frediansyah Firdaus

Keyword(s):

Data Analysis ◽

Analysis Of Variance ◽

Research Method ◽

Moringa Oleifera ◽

The Third ◽

Randomized Design ◽

Leaf Powder ◽

Completely Randomized Design ◽

Second Period

ABSTRAK Penelitian bertujuan untuk mengetahui pengaruh pemberian bubuk daun Moringa oleifera (MO) terhadap kuantitas dan kualitas semen sapi peranakan ongole (PO). Metode penelitian menggunakan percobaan lapang dengan rancangan acak lengkap dengan tiga perlakuan pemberian MO sebesar 0 kg/ekor/hari (P1); 0,05 kg/ekor/hari (P2) dan 0,1 kg/ekor/hari (P3). Analsis data menggunakan one way analysis of variance. Masing-masing perlakuan terdiri atas empat ekor dengan tiga periode pengamatan sebagai ulangan, yaitu periode pertama dan ketiga tanpa MO, dan periode kedua diberikan MO pada P2 dan P3. Hasil penelitian menunjukan bahwa volume dan pH semen perlakuan P1, P2 dan P3 tidak berbeda semua periode pengamatan. Motilitas massa spermatozoa P2 dan P3 periode kedua dan ketiga menunjukan nilai +++ berbeda nyata (P<0,05) dibandingkan dengan P1 (++); motilitas individu perlakuan P1 menunjukkan nilai terendah 60,56±2,94 % (P<0,05) dibandingkan P2 dan P3 pada periode kedua dan ketiga (75,50±2,29 dan 72,50±2,34%). Konsentrasi spermatozoa P2 dan P3 periode kedua dan ketiga menunjukkan rata-rata >1.350 juta/ml berbeda nyata (P<0,05) daripada P1 (876±152juta/ml), sedangkan total spermatozoa motil menunjukkan nilai tertinggi 5.647±829 juta/ml pada P3 periode kedua.Viabilitas spermatozoa P2 dan P3 menunjukkan nilai >88-89 % beda nyata (P<0,05) daripada P1 (<84 %) pada periode kedua dan ketiga, sedangkan nilai abnormalitas spermatozoa yang terbaik adalah P2 sebesar 4,30% pada periode kedua dan P3 sebesar 5,33% pada periode ketiga. Disimpulkan pemberian bubuk daun MO dengan dosis 0,1 kg/ekor/hari dapat memperbaiki kuantitas dan kualitas semen (viabilitas dan total spermatozoa motil) sapi pejantan PO.Kata kunci: kuantitas dan kualitas semen, Moringa oleifera, sapi pejantan ABSTRACTThe research aimed to determine the effect of Moringa oleifera (MO) leaf powder on the quantity and quality of semen of ongole crossbreed. The research method used was a field experiment with a completely randomized design with three treatments giving MO of 0 kg/head/day (P1); 0.05 kg/head/day (P2) and 0.1 kg/head/day (P3). Each treatment consisted of four heads with three periods of observation as replications, namely the first and third periods without MO, and the second period was given MO on P2 and P3. Data analysis used a one-way analysis of variance. The results showed that the volume and pH of semen treated P1, P2, and P3 didn’t different in all observation periods. The second and third period spermatozoa mass motility of P2 and P3 showed that the value of +++ was significantly different (P<0.05) compared to P1 (++); Individual motility of P1 treatment showed the lowest value 60,56±2,94 % (P<0.05) compared to P2 and P3 in the second and third periods (75,50±2,29 and 72,50±2,34%). The second and third periods of P2 and P3 spermatozoa concentrations showed an average of >1,350 million/cc significantly different (P<0.05) than P1 (876±152 million/cc), while the total motile spermatozoa showed the highest value of 5,647±829 million/cc in the second period P3. The spermatozoa viability of P2 and P3 showed values >88-89% significantly different (P<0.05) than P1 (<84%) in the second and third periods, while the best spermatozoa abnormalities were P2 at 4.30% in the second period and P3 at 5.33% in the third period. It was concluded that the giving of MO leaf powder at a dose of 0,1 kg/head/day could improve the quantity and quality of semen (viability and total motile spermatozoa) of Ongole Crossbreed bulls. Keywords: bulls, Moringa oleifera, quantity and quality of semen

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Nutrient and inorganic solute (Na+ and Cl-) content in green onion plants under hydroponic cultivation using brackish water

Ciência e Agrotecnologia ◽

10.1590/1413-7054202044013320 ◽

2020 ◽

Vol 44 ◽

Author(s):

Carlos Donato da Silva Souza ◽

Geronimo Ferreira da Silva ◽

Sirleide Maria de Menezes ◽

José Edson Florentino de Morais ◽

José Amilton Santos Júnior ◽

...

Keyword(s):

Nutrient Solution ◽

Brackish Water ◽

Hydroponic System ◽

Brackish Waters ◽

Randomized Design ◽

Hydroponic Cultivation ◽

Salinity Levels ◽

Completely Randomized Design ◽

Harmful Effects ◽

Different Levels

ABSTRACT Cultivation using brackish waters can result in nutritional and metabolic imbalances in several plant species, consequently reducing the production of dry matter (DM) and accumulation of toxic ions (Na+ and/or Cl-) in plants. We evaluated the DM production, and nutrient and inorganic solute (Na+ and Cl-) content in green onion plants (cv. Todo Ano Evergreen - Nebuka) under different levels of nutrient solution salinity in combination with circulation frequencies of this solution. Two experiments were conducted in a hydroponic system, using a completely randomized design, in a 6 × 2 factorial scheme, with five replicates: six levels of nutrient solution salinity (1.5, 3.0, 4.5, 6.0, 7.5, and 9.0 dS m-1) and two solution circulation frequencies (twice and thrice a day). In Experiment I, the evapotranspired depth was replaced using brackish water that was used to prepare each of the salinity levels (used exclusively), whereas in Experiment II, brackish water was used only to prepare each of the salinity levels and public water was used (electrical conductivity [ECw] = 0.12 dS m-1) for replacement in all treatments. The increase in the nutrient solution salinity reduced the production of DM and accumulation of nutrients; the reductions were more pronounced when brackish waters were used exclusively (Experiment I). However, the circulation of solutions thrice a day resulted in the harmful effects of the salinity effect. Replacing the evapotranspirated blade with water supply (Experiment II) mitigated the deleterious effects of salinity. Moreover, three circulations of the nutrient solution daily resulted in lower accumulation of inorganic Na+ and Cl- solutes and increased accumulation of nutrients N, P, K+, Ca2+, Mg2+, and S in the culture.

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The quality properties, thiobarbituric acid (TBA) values and microstructure of chicken sausage with local red beetroot powder

Food Research ◽

10.26656/fr.2017.5(s2).019 ◽

2021 ◽

Vol 5 (S2) ◽

pp. 113-119

Author(s):

W. Swastike ◽

E. Suryanto ◽

Rusman ◽

C. Hanim ◽

Jamhari ◽

...

Keyword(s):

Peroxide Value ◽

Room Temperature ◽

Thiobarbituric Acid ◽

Quality Properties ◽

Randomized Design ◽

Powder Addition ◽

Completely Randomized Design ◽

Different Levels ◽

Temperature Storage

This research was aimed to determine the quality properties, the microstructure of chicken sausage and Thiobarbituric acid (TBA) values with locally Indonesia red beetroot powder. The main ingredients of chicken sausage-making in this research were broiler chicken, filler, binder, beetroot powder, and spices. Red beetroot powder function as a filler was substituted tapioca starch in chicken sausage batter in three different levels. The combination of red beetroot powder with level 0, 1.0, 2.0 and 3.0% of total batter and shelf life at room temperature for 0, 1, 2 and 3 days. Each treatment consisted of five replications. The variables observed using quality properties (moisture, ash, fat, protein, crude fiber and calorie), microstructure and peroxide value of chicken sausage. The data of quality properties and peroxide value were analyzed by using one-way analysis (ANOVA) of Completely Randomized Design. The differences between means were analyzed by Duncan's New Multiple Ranges Test. The data of microstructure was analyzed by descriptive analyses. The moisture, protein, fat and ash contents for chicken sausages were significantly different (p<0.05). The chicken sausage with 2% substitution of beet powder produced chicken sausages with a high protein content of 14.77±0.02% while a low-fat content is 0.42±0.01%. Thiobarbituric acid (TBA) values of chicken sausages increased throughout the three days of room temperature storage (38°C). Chicken sausage formulated with red beetroot powder showed a significantly lower TBA value compared to the samples without red beetroot powder (p<0.05). In conclusion, a higher level of beetroot powder will improve the quality of chicken sausage and also the microstructure. The best level of beetroot powder addition was 2.0%. The addition of beetroot powder able to maintain fresh sausage conditions up to 2 days of storage at room temperature.

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Physicochemical, Microbiological and Sensory Properties of Fermented Whey using Kombucha Inoculum

Buletin Peternakan ◽

10.21059/buletinpeternak.v43i1.31496 ◽

2019 ◽

Vol 43 (1) ◽

Author(s):

Fitri Suciati ◽

Nurliyani Nurliyani ◽

Indratiningsih Indratiningsih

Keyword(s):

Green Tea ◽

Black Tea ◽

Acetic Acid Bacteria ◽

Solid Content ◽

Sensory Properties ◽

Whey Protein Concentrate ◽

Plate Count ◽

Randomized Design ◽

Completely Randomized Design ◽

Different Levels

This research aimed to determine physicochemical, microbiological and sensory properties of fermented whey using kombucha inoculum. The material used were kombucha, black tea, green tea, and whey protein concentrate. The research used factorial completely randomized design. The first factor was kombucha inoculum using different mediums (black and green tea) at different levels (5; 10; 15; dan 20%) as a second factor. The whey was fermented at 37oC for 39 hours. Research showed the use of kombucha inoculum using different medium (black and green tea) at different levels (5; 10; 15; dan 20%) did not affect (p>0.05) on microbiological properties (the number of Total Plate Count, total lactic acid bacteria, total acetic acid bacteria, and total yeast), dissolved protein content, lactose content, viscosity and acceptability. Black and green tea kombucha inoculum can be used in whey fermentation. Black tea kombucha fermented whey with 20% level addition has the best solid content. Relatively, kombucha fermented whey is quite acceptable.

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