scholarly journals In vivo test of Litopenaeus vannamei infected by Vibrio using Moringa oleifera leaf extract

DEPIK ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 86-90
Author(s):  
Iko Imelda Arisa ◽  
Cut Mutia ◽  
Sri Agustina ◽  
Nurfadillah Nurfadillah ◽  
Sofyatuddin Karina
Keyword(s):  
Litopenaeus Vannamei ◽  
Antibacterial Agent ◽  
Leaf Extract ◽  
Moringa Oleifera ◽  
In Vivo Test ◽  
Weight Growth ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Performance Results

This study aimed to determine the optimal concentration of Moringa oleifera leaf extract as an antibacterial agent on Litopenaeus vannamei larvae that are infected by Vibrio sp.. It was conducted in 15 March - 15 April 2019 at PT. Global Gain Superior Seeds, Pantai Cermin, North Sumatera. The research used a completely randomized design (CRD) method with five treatments and four replications. The shrimps infected by Vibrio sp. were immersed into M. oleifera leaf extract in various concentration of (A) 0 ppm, (B) 400 ppm, (C) 600 ppm, (D) 800 ppm, and (E) 1000 ppm for ±15 minutes. The size of shrimp used was post-larvae 8 (length of 3.5-5 mm/ind). The best performance results of vannamei shrimps were obtained in treatment E (1000 ppm), namely length growth of 16.75 ± 1.16 cm, weight growth of 0.92 ± 0.89 g and survival rate of 91±7.87%.Keywords:Moringa oleiferaVibrio sp.Immersion

scholarly journals Utilization of Paku Uban (Nephrolepis biserrata) Extract as a Molting Stimulant of Mud Crabs (Scylla spp.) in Traditional Ponds

2020 ◽  
Vol 12 (1) ◽  
pp. 113
Author(s):  
Diana Maulianawati ◽  
Rukisah Rukisah ◽  
Awaludin Awaludin ◽  
Muhammad Iswan Guntur
Keyword(s):  
Survival Rate ◽  
Leaf Extract ◽  
Economic Value ◽  
Highest Weight ◽  
Weight Growth ◽  
Molting Process ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Different Doses ◽  
Mud Crabs

HighlightsThe Paku Uban (Nephrolepis biserrata) was identified and analyzedThe ability of paku uban to stimulated molting process of Scylla sp was analyzedThe paku uban (Nephrolepis biserrata) could increase the presentation of molting and accelerate molting processAbstractMud crab (Scylla spp.) is a fishery commodity with high economic value. For this reason, efforts to increase production are required with a more effective applicative technology. This study aims to determine the molting response and survival rate of mud crabs injected with paku uban leaf  extract (N. biserrata) at different doses. The study was conducted for 30 days, located in Tibi Island, Tanah Lia District, Tanah Tidung. The design used in this study was a completely randomized design (CRD) with five treatments and eight replicates. Mud crabs weighing 80 - 150 g were tested in K1 (without injection), K2 (controlled injection), P1 (100 ppm extract), P2 (125 ppm extract) and P3 (150 ppm extract). The effectivity of paku uban (N. biserrata) extract with the highest percentage of molting of 50% took place at P3, 37.5% at P2, and 25% at P1. The lowest levels of molting percentage were at K1 and K2 by 0%. The fastest molting period was ten days at P2, and the longest was 29 days at P1. The highest weight growth reached 33.75 g at P3, followed by P2 and P1 with 31 and 18.75 g, respectively. The survival rate of mud crabs for all treatments reached 100%. The results of the analysis of variance indicated a significant bet effect (P < 0.05) on the molting and weight growth percentage. The BNT test suggested that a dose of 150 ppm was the optimum treatment. 

scholarly journals UNTILIZATION OF Moringa oleifera LEAF EXTRACT ON DECREASING INFESTATION OF Argulus japonicus IN GOLDFISH (Carassius auratus)

AQUASAINS ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 769
Author(s):  
Febri Setyawati ◽  
Kismiyati Kismiyati ◽  
Sri Subekti
Keyword(s):  
Carassius Auratus ◽  
Leaf Extract ◽  
Moringa Oleifera ◽  
Optimal Concentration ◽  
Percentage Decrease ◽  
Goldfish Carassius Auratus ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Range Test ◽  
Moringa Leaf Extract

Abstrak Goldfish (Carassius auratus) is one of the fishes that can be infected by parasites. Commonly, this fish is often attacked by Argulus japonicus ectoparasites. Control of infestation in A. japonicus can be done by utilizing one of the natural ingredients, Moringa oleifera leaf extract, which contains of alkaloids, tannins, saponins and flavonoids. This study aims to determine the effect of M. oleifera leaf extract in reducing the infestation of A. japonicus and the optimal concentration of M. oleifera leaf extract in reducing the infestation of A. japonicus in goldfish (C. auratus). This study used a completely randomized design (CRD) with treatments namely control, 600 ppm, 700 ppm, 800 ppm and 900 ppm with four times of replication. The main parameter is the percentage decrease in infestation of A. japonicus. The results of the research data were analyzed by using ANOVA and followed with the Ducan Multiple Range Test. The results showed that Moringa leaf extract could reduce the infestation of A. japonicus. The optimal concentration of Moringa leaf extract in reducing the infestation of A. japonicus was obtained from a concentration of 700 ppm.

scholarly journals Addition of bidara leaves (Ziziphus spina – christi L.) in drinking water on production and mortality of broiler chickens

2021 ◽  
Vol 902 (1) ◽  
pp. 012045
Author(s):  
F H Yusup ◽  
N Frasiska ◽  
N Rahayu
Keyword(s):  
Broiler Chickens ◽  
Leaf Extract ◽  
The Body ◽  
Production Performance ◽  
Physiological Processes ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Usage Level ◽  
Average Body

Abstract The bidara plant is known as a plant that contains active ingredients and phytochemicals that have the benefit of stimulating physiological processes in the body to run well and have a healthy effect. This study aimed to determine the effect of giving bidara leaf extract on the production performance and mortality of broiler chickens. A total of 60 DOC broiler chickens were used in this study with an average body weight of 45 g which was divided into 4 treatments, including T0: control, T1: 10% bidara leaf extract, T2: 15% bidara leaf extract, T3: 20% bidara leaf extract. Each treatment was repeated 5 times with 3 research units per plot. The study was carried out experimentally in vivo using the Completely Randomized Design (CRD) research method. The data was processed by analysis of variance with the F test at the 5% level and continued with Duncan’s test. The results showed that the treatment had a significant effect (P<0.05) on production performance and mortality in broiler chickens at the 10% bidara leaf usage level. The use of bidara leaf extract can be used as a supplement in the maintenance of broiler chickens as a substitute for synthetic supplements.

scholarly journals Effect of synthetic or herbal preservatives on the quality of beef meatballs at different shelf life periods

2018 ◽  
Vol 16 (1) ◽  
pp. 23-34
Author(s):  
F Islam ◽  
MA Hossain ◽  
MF Rahman ◽  
MA Hashem ◽  
M Rahman ◽  
...  
Keyword(s):  
Leaf Extract ◽  
Moringa Oleifera ◽  
Safety Evaluation ◽  
Nutrient Quality ◽  
Overall Acceptability ◽  
Randomized Design ◽  
Microbial Analysis ◽  
Completely Randomized Design ◽  
Moringa Leaf Extract

The experiment was conducted to compare the effect of Moringa oleifera leaf extract with synthetic antioxidant on beef meatball. Five types of beef meatballs were formulated for this purpose. Meatballs were made with control (0%), 0.1% Beta Hydroxyl Anisole (BHA), 0.1, 0.2 and 0.3% Maringa oleifera leaf extract, respectively. Quality and safety evaluation of meatballs were determined by sensory, physiochemical, biochemical and microbiological tests. After preparation meatballs were preserved at -20˚C. The analyses were conducted at 0, 15t, 30 and 60 days of interval. An ANOVA of a 5x4 factorial experiment in completely randomized design having three replications per treatment was used for the analyses of data. Considering CP, tenderness, juiciness, overall acceptability, cooking loss, FFA, POV and TBARS value it can be concluded that Moringa oleifera leaf extract up to a level of 0.3% may replace BHA for meatball preservation without deteriorating its quality. In case of sensory evaluation 0.2% Moringa leaf extract shows better results. But on the basis of nutrient quality, physicochemical properties, biochemical analysis and microbial analysis 0.3% Moringa leaf extract group is more satisfactory than other treatment groups.SAARC J. Agri., 16(1): 23-34 (2018)

scholarly journals KAJIAN EFEKTIVITAS EKSTRAK GEL IKAN TOMAN (Channa micropeltes) PADA LUKA BAKAR MENCIT (Mus musculus)

2019 ◽  
Vol 15 (2) ◽  
pp. 184
Author(s):  
Firlianty Firlianty ◽  
Hermansyah Hermansyah ◽  
Windarina Samosir
Keyword(s):  
Data Analysis ◽  
Experimental Method ◽  
Mus Musculus ◽  
Control Treatment ◽  
Test Results ◽  
Male Mice ◽  
In Vivo Test ◽  
Randomized Design ◽  
Completely Randomized Design

This study aims to determine the study of the effectiveness of toman fish (Channa micropeltes) gel extract on healing burns of mice (Mus musculus). This research was conducted for 4 months, starting from September 2018 to January 2019. This study used the experimental method and data analysis using a completely randomized design with 4 children and three replications: treatment A as control, treatment B giving toman fish extract gel (Channa micropeltes ) 1.5%, treatment C 3% and treatment D 4.5%. The results showed that on day 4, day 8 and day 12 showed that the administration of gel extract had a significant effect on changes in the length of burns of mice (Mus musculus) (P>0.05). In vivo test results on male mice with toman fish gel extract can capture the healing of burns in mice.

scholarly journals Effect Concentration of Moringa (Moringa oleifera Lam) Leaf Extract in Citrate-Egg Yolk in Maintaining Motility and Viability of Spermatozoa of Kacang Goat

2021 ◽  
Vol 16 (4) ◽  
pp. 340-346
Author(s):  
R. R. Dapawole ◽  
I. P. Sirappa
Keyword(s):  
Leaf Extract ◽  
Moringa Oleifera ◽  
Sperm Concentration ◽  
Egg Yolk ◽  
Progressive Motility ◽  
Randomized Design ◽  
Effect Concentration ◽  
Completely Randomized Design ◽  
Artificial Vagina ◽  
Moringa Oleifera Lam

This study aimed to determine the effectiveness and the best concentration of Moringa leaf extract (MLE) in the citrate-egg yolk (C-EY) to maintain the motility and viability of spermatozoa kacang goat. Semen was collected from 3 goats aged two years; by using the artificial vagina method. The semen was evaluated macroscopically and microscopically. The semen that had >70% sperm motility and >250x106/ml sperm concentration was divided into 4 equal tubes, each diluted with100% C-EY (P1), 10% MLE+ 90%C-EY (P2), 20% MLE +80% C-EY (P3), and 30% MLE+70% C-EY (P4). The diluted samples were then stored in a refrigerator (3-5?C) and evaluated for motility and viability every 24 hours. The study was designed using a completely randomized design (CRD) consisting of four treatments and five replications. The results showed that the addition of MLE in C-EY significantly affected goat spermatozoa's progressive motility and viability. The data showed that the spermatozoa kept during four days in a diluent of P2 had higher (P<0.05) motility 44.67±4.80% and viability 74.24±4.46%than the other three diluents of P1(36.00±4.70%; 70.10±3.6%), P3(33.67±0.42%; 66.85±4.99%) and P4 (29.67±3.99%; 63.96±5.44%). This study concluded that adding 10% MLE was the best concentration as source energy in 90% C-EY diluents, which effectively maintained the motility and viability of kacang goat spermatozoa for four days of storage at a temperature of 3-5oC.

scholarly journals Fungi associated with Paspalum guenoarum seeds: their impact on physiology and control

2021 ◽  
Vol 51 (9) ◽  
Author(s):  
Bruno Frosi Gasparetto ◽  
Lauri Lourenço Radunz ◽  
Rodrigo Ramos Lopes ◽  
Lúcia Brandão Franke ◽  
José Antônio Martinelli
Keyword(s):  
In Vivo Test ◽  
Chemical Treatments ◽  
Tukey Test ◽  
In Vitro Sensitivity ◽  
Randomized Design ◽  
Qualitative Variables ◽  
Completely Randomized Design ◽  
And Control

ABSTRACT: Of the natural pastures grown in southern Brazil, those of the genus Paspalum are the most important. One of the factors that hinder their cultivation is the availability of quality seeds, that are often compromised by the presence of fungi. This study determined the in vitro sensitivity of Paspalum guenoarum ecotype azulão seed-associated fungus to certain fungicides and to measure the efficiency of chemical treatments for fungal control and seed physiological performance. Bipolaris micropus, Epicoccum sorghinum, Curvularia geniculata and Fusarium incarnatum associated with seeds were tested in vitro against Carbendazim; Tiram, Fludioxonil; Metalaxil-M, Carboxina; Tiram and Tiofanato-metílico at 0, 1, 2.5, 5, 10 and 30 µg/mL. This experiment was conducted in a completely randomized design (factorial 4 × 4 × 6). Qualitative variables were compared using the Tukey test (P ≤ 0.05) and quantitative variables were subjected to regression analysis. Carbendazim; Tiram and Fludioxonil; Metalaxil-M had the best fungicidal performances, each inhibiting three of the four fungi with LD50s below 1 µg/mL. Subsequently, these two fungicides were used alone or in combination in the in vivo test. A completely randomized design was used and the means were compared using the Tukey test (P < 0.05). The chemical treatment of the seeds resulted in improvement of five of the six evaluated physiological parameters. The identification of the primary fungi associated with Paspalum seeds reported in this research, as well as damage done to them, can be diminished using appropriate measures such as seed treatments.

scholarly journals OTIMIZAÇÃO DAS CONDIÇÕES DE ENSAIO IN VIVO DA ENZIMA REDUTASE DO NITRATO EM CUCURBITÁCEAS

Nativa ◽  
2021 ◽  
Vol 9 (1) ◽  
pp. 09-15
Author(s):  
Biank Amorim Rodrigues ◽  
Alessandro Carlos Mesquita
Keyword(s):  
Nitrate Reductase ◽  
Plant Material ◽  
Leaf Tissue ◽  
In Vivo Test ◽  
Test Conditions ◽  
Randomized Design ◽  
Leaf Tissues ◽  
Completely Randomized Design ◽  
Different Cultures

As cucurbitáceas abrangem espécies de importância para o Nordeste brasileiro, dentre elas o melão, a melancia e o pepino são as que possuem maior expressividade de cultivo. O sistema de produção dessas hortaliças requer a presença de nitrogênio, que, sendo que a redução do nitrato por meio da atividade da redutase do nitrato no citosol da célula é uma parte importante do processo de incorporação do N em aminoácidos. Contudo, as metodologias utilizadas são diversas e aplicadas em diversas culturas de forma geral, havendo a necessidade de uma maior especificidade. O presente trabalho teve como objetivo padronizar as condições mais apropriadas para a determinação da atividade da RN, in vivo, no tecido foliar de melancia, melão e pepino. O experimento foi conduzido em delineamento inteiramente casualizado em fatorial 3x3, compreendendo três metodologias in vivo e três propostas de infiltração do material vegetal (banho-maria, estufa e a vácuo), com quatro repetições.  As plantas foram conduzidas em casa de vegetação e os tecidos foliares completamente expandidos foram coletados, após 20 dias da germinação, para a condução dos métodos enzimáticos e avaliar a atividade da enzima redutase do nitrato (NR). Os resultados obtidos demonstraram que a maior atividade da NR no tecido foliar de melancia e melão foi obtida utilizando a Metodologia 1 submetido ao vácuo, enquanto que para o pepino o melhor ensaio foi o Metodologia 2, quando também submetido ao vácuo. Palavras-chave: nitrogênio; metabolismo; enzimologia.   Optimization of in vivo test conditions of nitrate enzyme reductase in Cucurbitacea   ABSTRACT: Cucurbits include species of importance to the Northeast of Brazil, among them melon, watermelon and cucumber are those that have the most expressive cultivation. The production system of these vegetables requires the presence of nitrogen, which, whereas the reduction of nitrate through the activity of nitrate reductase in the cell's cytosol is an important part of the process of incorporating N into amino acids. However, the methodologies used are diverse and applied in different cultures in general, with the need for greater specificity. The present study aimed to standardize the most appropriate conditions for determining the activity of RN, in vivo, in the leaf tissue of watermelon, melon and cucumber. The experiment was conducted in a completely randomized design in a 3x3 factorial, comprising three in vivo methodologies and three proposals for infiltration of plant material (water bath, greenhouse and vacuum), with four replications. The plants were conducted in a greenhouse and the fully expanded leaf tissues were collected, after 20 days of germination, to conduct the enzymatic methods and evaluate the activity of the nitrate reductase (NR) enzyme. The results obtained demonstrated that the greatest activity of NR in the watermelon and melon leaf tissue was obtained using Methodology 1 submitted to vacuum, while for cucumber the best test was Methodology 2, when also submitted to vacuum. Keywords: nitrogen; metabolismo; enzimology.

scholarly journals Pengaruh jenis pelarut pada ekstrak daun kelor (Moringa oleifera) terhadap mortalitas larva Aedes aegypti

2019 ◽  
Vol 4 (02) ◽  
pp. 101-109
Author(s):  
Restiani Sih Harsanti ◽  
Ratna Mustika Yasi
Keyword(s):  
Aedes Aegypti ◽  
Leaf Extract ◽  
Moringa Oleifera ◽  
Mortality Data ◽  
Leaf Extracts ◽  
Alternative Ingredients ◽  
Randomized Design ◽  
Research Findings ◽  
Completely Randomized Design ◽  
Moringa Leaf Extract

Innovation is needed using alternative ingredients as natural larvacide instead of temefos. One of the plants that can be used for making natural larvacide is Moringa (Moringa oleifera) which contains alkaloids and flavonoids. This study aims to determine the effect of the type of solvent on Moringa (M. oleifera) extract on the mortality of Aedes aegypti larvae and determine the most effective solvent in extracting Moringa leaves  as A. aegypti larvacide. This study uses a completely randomized design with 5 replications. Moringa leaf extract is obtained by maceration with aquades, ethanol, methanol, ethyl acetate, and n-hexane. Moringa leaf extracts were tested for phytochemistry and larvacidal power was tested for A. aegypti larvae. Mortality data were analyzed by ANOVA and Tukey tests. The results showed that the type of solvent in moringa leaf extract (M. oleifera) did not significantly influence the mortality of A. aegypti larvae and the most effective type of solvent for extraction of Moringa leaves were aquades and ethanol. Based on the research findings it can be concluded that the type of solvent in moringa leaf extract does not significantly influence the mortality of A. aegypti larvae. However, the most effective types of solvents for extraction of Moringa leaves are aquades and ethanol.

Pengaruh Pemberian Moringa oleifera Terhadap Kuantitas dan Kualitas Semen Sapi Peranakan Ongole

2020 ◽  
Vol 7 (2) ◽  
pp. 119
Author(s):  
Lukman Affandhy ◽  
Muchamad Luthfi ◽  
Dian Ratnawati ◽  
Frediansyah Firdaus
Keyword(s):  
Data Analysis ◽  
Analysis Of Variance ◽  
Research Method ◽  
Moringa Oleifera ◽  
The Third ◽  
Randomized Design ◽  
Leaf Powder ◽  
Completely Randomized Design ◽  
Second Period

ABSTRAK  Penelitian bertujuan untuk mengetahui pengaruh pemberian bubuk daun Moringa oleifera (MO) terhadap kuantitas dan kualitas semen sapi peranakan ongole (PO). Metode penelitian menggunakan percobaan lapang dengan rancangan acak lengkap dengan tiga perlakuan pemberian MO sebesar 0 kg/ekor/hari (P1); 0,05 kg/ekor/hari (P2) dan 0,1 kg/ekor/hari (P3). Analsis data menggunakan one way analysis of variance. Masing-masing perlakuan terdiri atas empat ekor dengan tiga periode pengamatan sebagai ulangan, yaitu periode pertama dan ketiga tanpa MO, dan periode kedua diberikan MO pada P2 dan P3. Hasil penelitian menunjukan bahwa volume dan pH semen perlakuan P1, P2 dan P3 tidak berbeda semua periode pengamatan. Motilitas massa spermatozoa P2 dan P3 periode kedua dan ketiga menunjukan nilai +++ berbeda nyata (P<0,05) dibandingkan dengan P1 (++); motilitas individu  perlakuan P1 menunjukkan nilai terendah 60,56±2,94 % (P<0,05) dibandingkan P2 dan P3 pada periode kedua dan ketiga (75,50±2,29 dan 72,50±2,34%). Konsentrasi spermatozoa P2 dan P3 periode kedua dan ketiga menunjukkan rata-rata  >1.350 juta/ml berbeda nyata (P<0,05) daripada P1 (876±152juta/ml), sedangkan total spermatozoa motil menunjukkan nilai tertinggi 5.647±829 juta/ml pada P3 periode kedua.Viabilitas spermatozoa P2 dan P3 menunjukkan nilai >88-89 % beda nyata (P<0,05) daripada P1 (<84 %) pada periode kedua dan ketiga, sedangkan nilai abnormalitas spermatozoa yang terbaik adalah P2 sebesar 4,30% pada periode kedua dan P3 sebesar 5,33% pada periode ketiga. Disimpulkan pemberian bubuk daun MO dengan dosis 0,1 kg/ekor/hari dapat memperbaiki kuantitas dan kualitas semen (viabilitas dan total spermatozoa motil) sapi pejantan PO.Kata kunci:  kuantitas dan kualitas semen, Moringa oleifera, sapi pejantan ABSTRACTThe research aimed to determine the effect of Moringa oleifera (MO) leaf powder on the quantity and quality of semen of ongole crossbreed. The research method used was a field experiment with a completely randomized design with three treatments giving MO of 0 kg/head/day (P1); 0.05 kg/head/day (P2) and 0.1 kg/head/day (P3). Each treatment consisted of four heads with three periods of observation as replications, namely the first and third periods without MO, and the second period was given MO on P2 and P3. Data analysis used a one-way analysis of variance. The results showed that the volume and pH of semen treated P1, P2, and P3 didn’t different in all observation periods. The second and third period spermatozoa mass motility of P2 and P3 showed that the value of +++ was significantly different (P<0.05) compared to P1 (++); Individual motility of P1 treatment showed the lowest value 60,56±2,94 % (P<0.05) compared to P2 and P3 in the second and third periods (75,50±2,29 and 72,50±2,34%). The second and third periods of P2 and P3 spermatozoa concentrations showed an average of >1,350 million/cc significantly different (P<0.05) than P1 (876±152 million/cc), while the total motile spermatozoa showed the highest value of 5,647±829 million/cc in the second period P3. The spermatozoa viability of P2 and P3 showed values >88-89% significantly different (P<0.05) than P1 (<84%) in the second and third periods, while the best spermatozoa abnormalities were P2 at 4.30% in the second period and P3 at 5.33% in the third period. It was concluded that the giving of MO leaf powder at a dose of 0,1 kg/head/day could improve the quantity and quality of semen (viability and total motile spermatozoa) of Ongole Crossbreed bulls. Keywords: bulls, Moringa oleifera, quantity and quality of semen

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