scholarly journals Cultivation of mycelium of the edible fungus Pleurotus ostreatus (oyster mushroom) with the use of wet sparging grain, the waste of the brewing industry.

2021 ◽  
Author(s):  
Georgy V. Pestsov ◽  
Anastasia V. Glazunova ◽  
Yuriy M. Atroshchenko ◽  
Roman A. Sidorov
Keyword(s):  
Pleurotus Ostreatus ◽  
Waste Product ◽  
Fruit Body ◽  
Oyster Mushroom ◽  
Brewing Industry ◽  
Nutrient Media ◽  
Mycelium Growth ◽  
Edible Fungus ◽  
The Russian Federation ◽  
Intensive Growth

Abstract Wet sparging grain, a waste product of brewery, was shown to be successfully utilized as a supplement to the nutrient media and substrates for both isolation of the vegetative mycelium of the Pleurotus ostreatus mushroom, as well as for its further recovery and growing the carposomes. We studied the influence of the wet sparging grain on mycelium growth. Cultivation results demonstrated that wet sparging grain is an efficient additional component to the minimal agarized nutrient media for P. ostreatus growth and isolation and to its common fruit body cultivation substrates. Even the depleted agaric media and substrates with addition of the sparging grain allowed intensive growth of the P. ostreatus mycelium and its fructifying. Practical Application: Addition of wet sparging grain to poor cheap substrates increases their quality for rearing the edible oyster mushroom mycelium to the level of reach substrates, and allows utilization of agricultural wastes. The technique is ready for application and patented in the Russian Federation.

scholarly journals Pemanfaatan Limbah Serbuk Teh sebagai Substitusi Serbuk Gergaji terhadap Pertumbuhan Miselium dan Produksi Jamur Tiram Putih (Pleurotus Ostreatus)

2018 ◽  
Vol 3 (1) ◽  
pp. 27
Author(s):  
Siti Mardiana ◽  
Ellen L Panggabean ◽  
Retno A Kuswardani ◽  
Muhammad Usman
Keyword(s):  
Fruiting Body ◽  
Pleurotus Ostreatus ◽  
Fruit Body ◽  
Oyster Mushroom ◽  
Combined Treatments ◽  
Mycelium Growth ◽  
Growing Media ◽  
Randomized Design ◽  
Tea Waste ◽  
Completely Randomized Design

<p class="HowToCite"><em>The waste of tea powder is abundant and has not been widely used optimally. The alternative use is to use the waste as an additional medium or substitution for the white oyster mushroom growing media. The purpose of the study was to determine the effect of mycelium growth and the production of the fruit of the white oyster mushroom on combination media / tea powder substitution with sawdust. The research design used was a completely randomized design (CRD) with 4 replications. Combined treatments were M0 = 100% sawdust; M1 = 100% Tea waste powder; M2 = 75% tea powder + 25% sawdust; M3 = 50% tea powder + 50% sawdust and M4 = 25% tea powder + 75% sawdust. All treatments used the composition of the manufacture of mushroom growing media consisting of each combination of treatments, bran 10% by weight of the material and lime 0.5% by weight of the material. The parameters observed were the growth of white oyster mushroom mycelium, the rate of mycelium growth, the time the mycelium closed the substrate, the time of appearance of the fruiting body, the weight of the fruit body and the diameter of the fruit hood. The results showed that all parameters differed significantly. The best treatment is in treatment M4, followed by treatment of M3, M0 and M2.</em></p>

scholarly journals Pengaruh penambahan serbuk jagung pada komposisi media tanam terhadap pertumbuhan miselium bibit F1 jamur tiram putih (Pleurotus ostreatus)

2021 ◽  
Vol 10 (2) ◽  
pp. 158-170
Author(s):  
Muhaeming Muhaeming ◽  
Jamilah Jamilah ◽  
Zulkarnaim Zulkarnaim
Keyword(s):  
Experimental Study ◽  
Analysis Of Variance ◽  
Pleurotus Ostreatus ◽  
Medium Composition ◽  
Oyster Mushroom ◽  
Control Treatment ◽  
Mycelium Growth ◽  
Randomized Design ◽  
Significant Difference ◽  
Completely Randomized Design

Penambahan nutrisi perlu dilakukan untuk meningkatkan produktivitas pertumbuhan jamur tiram putih (Pleurotus ostreatus). Salah satu bahan yang dapat digunakan adalah serbuk jagung. Penelitian ini bertujuan untuk mengetahui pengaruh penambahan serbuk jagung pada komposisi media tanam terhadap pertumbuhan miselium bibit F1 jamur tiram putih (Pleurotus ostreatus) dan mengetahui konsentrasi serbuk jagung yang paling baik digunakan pada pertumbuhan  miselium bibit F1 jamur tiram putih (Pleurotus ostreatus). Penelitian ini mengunakan Rancangan Acak Lengkap (RAL) 4 perlakuan dan 3 ulangan sehingga diperoleh 12 satuan percobaan; P0 (perlakuan kontrol), P1 (25%), P2 (50%), dan P3 (100%). Variabel yang diukur adalah persentase pertumbuhan miselium yang diamati pada hari ke-6, 12 dan 18 dan waktu penyebaran miselium Hari Setelah Inokulasi (HSI). Data hasil penelitian dianalisis menggunakan analysis of variance (ANOVA) dengan uji lanjut menggunakan uji Beda Nyata Terkecil (BNT). Hasil penelitian menunjukkan bahwa ada pengaruh pada pertumbuhan miselium bibit F1 jamur tiram putih (Pleurotus ostreatus) dengan penambahan serbuk jagung pada komposisi media tanam. Selain Itu, konsentrasi serbuk jagung yang memberikan hasil yang paling baik terhadap pertumbuhan miselium adalah P2 dengan konsentrasi 50%. Kata kunci : miselium F1; Pleurotus ostreatus; serbuk jagung  ABSTRACTAdditional nutrition needs to be done to increase the productivity of oyster mushroom (Pleurotus ostreatus) growth. One of the materials that can be used is corn powder. This study aimed to determine the effect of corn powder on the planting medium composition towards mycelium growth of oyster mushroom F1 seeds (Pleurotus ostreatus) and to find the best concentration of corn powder to grow mycelium seeds F1 oyster mushroom (Pleurotus ostreatus). This research was an experimental study using a Completely Randomized Design (CRD) with 4 treatments namely; P0 (control treatment), P1 (25%), P2 (50%), and P3 (100%), and 3 repetitions so that obtained 12 experimental units. The variables measured were the mycelium growth percentage on days 6, 12, and 18, and the mycelium widespread time after the day of inoculation. The data were analyzed using ANOVA then continued on the Least Significant Difference (LSD) test. The results showed that the addition of corn powder on oyster mushroom growing media affected mycelium growth of the oyster mushroom F1 seeds (Pleurotus ostreatus). It also found that the P2 with a 50% concentration of corn powder gave the widest mycelium growth.  Keywords: corn powder; mycelium; Pleurotus ostreatus

scholarly journals Produksi dan kualitas jamur tiram (Pleurotus ostreatus) pada beberapa konsentrasi limbah sludge pabrik kertas Production and quality of oyster mushroom (Pleurotus ostreatus) on selected concentration of sludge of paper industry

2016 ◽  
Vol 76 (2) ◽  
Author(s):  
Happy WIDIASTUTI ◽  
. TRI-PANJI
Keyword(s):  
Pleurotus Ostreatus ◽  
Paper Industry ◽  
Fruit Body ◽  
Raw Material ◽  
Oyster Mushroom ◽  
Biological Efficiency ◽  
Recycled Paper ◽  
Mushroom Production ◽  
Fe Content

Summary An experiment has been conducted to study the effect of sludge concentration,  waste of paper industry using raw material of recycled paper, as media on oyster mushroom production and quality. Twelve treatment tested are combination of two oyster mushroom strains are oyster mushroom of Bogor (JTB) and oyster mushroom of Taiwan (JTT), three media composition (sawdust, sludge, and sawdust+ sludge (50/50, v/v), and two levels of supplement addition (with rice bran+gypsum+ lime and without) with 10 replications. The production of the  mushroom was conducted  in bag log capacity of 1 kg fresh weight (water content 50%). The result showed that sludge can be used as mixture of oyster mushroom production with the composition 50:50 v/v of sawdust and sludge. Since the higher number of contamination, addition of supplement reduce oyster mushroom production as well as biological efficiency, but increased protein content of fruiting body. The content of Cd, and Pb were below the permissible limits, Cu was higher than the limits but still in the range. The Fe content of mushroom fruit body was higher both in sawdust (147.92 – 149.56 ppm) and sawdust+sludge (295.82 – 335.12 ppm) as media. However, the uptake of Fe of JTT was less (49.08-59.64 ppm) compared to that of JTB (147.92-335.12 ppm).Ringkasan Penelitian dilakukan untuk mempelajari pengaruh konsentrasi sludge limbah pabrik kertas berbahan baku karton bekas sebagai medium terhadap produksi dan kualitas jamur tiram. Dua belas perlakuan yang diuji merupakan kombinasi dua galur jamur tiram, yaitu Jamur Tiram Bogor (JTB) dan Jamur Tiram Taiwan (JTT), tiga jenis komposisi medium (serbuk gergaji, sludge, dan sludge+ serbuk gergaji), dan dua tingkat suplemen (dengan dan tanpa) yang diulang 10 kali untuk masing-masing perlakuan. Produksi jamur tiram dilakukan menggunakan bag log  berkapasitas 1 kg basah (kadar air 50%). Hasil percobaan menunjukkan bahwa sludge dapat digunakan sebagai campuran serbuk gergaji dalam produksi jamur tiram dengan per-bandingan 50:50 (v/v). Pemberian suplemen menurunkan produksi jamur tiram demikian pula efisiensi biologi namun meningkatkan kadar protein tubuh buah. Di dalam tubuh buah JTB, kandungan logam Cd, dan Pb berada di bawah batas yang diijinkan, sedangkan kandungan Cu di atas ambang walaupun masih dalam kisaran. Kandungan  Fe dalam tubuh buah jamur relatif tinggi baik yang ditumbuhkan pada serbuk gergaji sebagai medium standar (147,92 - 149,56 ppm) maupun yang ditumbuhkan pada medium campuran sludge+serbuk gergaji (295,82 - 335,12 ppm). Serapan Fe tubuh buah JTT jauh lebih rendah (49,08- 59,64 ppm) dibandingkan dengan serapan Fe JTB (147,92-335,12 ppm).  

scholarly journals USE OF ZPT AND PLANTING MEDIA ON THE GROWTH OF MYCELIUM OYSTER MUSHROOM (Pleurotus ostreatus)

2020 ◽  
Vol 7 (1) ◽  
pp. 2125-2131
Author(s):  
Joshua H.L. Tobing ◽  
Donn Ricky ◽  
Meyria K Situmeang
Keyword(s):  
Acetic Acid ◽  
Pleurotus Ostreatus ◽  
Water Extract ◽  
Boiling Water ◽  
Oyster Mushroom ◽  
Naphthalene Acetic Acid ◽  
Duncan Multiple Range Test ◽  
Green Beans ◽  
Mycelium Growth ◽  
Range Test

White Oyster mushroom (Pleurotus ostreatus) is an alternative food for the society because of its high nutrients content. This study uses of ZPT and media to see the grow of the mycelium white oyster mushroom. Media used are the boiling water extract of nuts (green beans, soy bean, peanuts, beans of string bean, and beans of nut snaps) and ZPT (Naphthalene Acetic Acid and Kinetin). Anova was used to analyze the data at a significant level of α = 0.05. The results shows that: (1) There’s a significant effect of ZPT and Non-ZPT on the mycelium growth with p=0.000, Duncan Multiple Range Test (DMRT) shows that Kinetin is highest contribution to the significancy of Anova; (2) planting media used in the study shows a significant differences on the mycelium growth with p=0039, Duncan Multiple Range Test shows that beans of long bean and soy bean are the highest contribution to the significancy of Anova; (3) time/days of observation  done in the study shows a significant differences on the mycelium growth with p=0000, Duncan Multiple Range Test shows that T6 or day 14 of observation shows the highest contribution to the significancy of Anova; (4) the interaction of ZPT, Non-ZPT and planting media show a significant differences on the mycelium growth p=0000; (5) the interaction of ZPT, Non-ZPT and time show a significant differences on the mycelium growth with p=0000; (6) the interaction of media and planting time show a significant differences on the mycelium growth with p=0000; and (7) the interaction of ZPT, Non-ZPT and planting media and time do not affect significantly the mycelium growth with  p=0053.

The Growth Of White Oyster Mushroom Mycelium (Pleurotus ostreatus) (Jacq) (P. Kumm) From Liquid And Solid Inoculum

Biocelebes ◽  
2019 ◽  
Vol 13 (3) ◽  
pp. 279-287
Author(s):  
Nisfaun Safitriana ◽  
Umrah ◽  
Orryani Lambui
Keyword(s):  
Liquid Medium ◽  
Incubation Time ◽  
Pleurotus Ostreatus ◽  
Solid Medium ◽  
Oyster Mushroom ◽  
Mycelium Growth ◽  
Average Growth ◽  
Solid Media ◽  
Randomized Design ◽  
Department Faculty

The study about  the growth of white oyster mushroom mycelium (Pleurotus ostreatus) (Jacq) (P. Kumm) from liquid and solid inoculum conducted on January until July 2018 in Biotechnology laboratory, Biology Department, Faculty of Math and Science, Tadulako University. This study was aimed to find out the mycelium growth of source of stem inoculum and hood in liquid and solid media. This study was designed by Complete Randomized Design (CRD) which consisted of four treatments and five replications. The treatment were M1 (source of stem inoculum in liquid medium), M2 (source of stem inoculum in solid medium), M3 (source of hood inoculum in liquid medium), and M4 (source of hood inoculum in solid medium). The parameters were (a) incubation time till mycelium fills medium, (b) CFU, and (c) the viability of inoculum on producing medium. The result showed that the faster incubation were M1 and M3 (for 2 days, higher CFU was M3 with an average number 8,2 x 10 10 CFU /ml. The faster viability of mycelium growth were M3 with an average growth of mycelium 6,97 cm/days.

scholarly journals Effects of Crude Oil on the Growth of Oyster Mushroom; Pleurotus ostreatus (Jacaum ex.fr. Kummer)

2019 ◽  
Vol 23 (10) ◽  
pp. 1787-1793
Author(s):  
F.A. Chukunda ◽  
W.S. Simbi-Wellington
Keyword(s):  
Crude Oil ◽  
Pleurotus Ostreatus ◽  
Oil Pollution ◽  
Fruit Body ◽  
Morphological Characteristics ◽  
Morphological Features ◽  
Oyster Mushroom ◽  
Mushroom Species ◽  
Crude Oil Pollution ◽  
Randomized Design

: The effect of crude oil on polluted sawdust on the growth of Pleurotus ostreatus was evaluated. The experiment was laid in a completely randomized design (CRD) with five replicates. Data collected were subjected to analysis of variance (ANVOA) and the mean separation was done using Duncan Multiple Range test (DMRT) at the probability of 5%. Result showed that micro-morphological characteristics of P. ostreatus grown on sawdust polluted with crude oil at varying concentrations of 50 ml, 100ml and 150ml and allowed to be contaminated for 8, 10 and 12 day respectively before sowing the mycelia in the polluted sawdust. Marco-Morphological at 10ml level of crude oil polluted sawdust significantly reduced the marco-morphological features of the P. ostreatus mushroom harvest at different days compared to 50ml crude oil polluted sawdust, in 8 days, the cap length measurement are (4.43cm±1.10 – 6.55cm±1.10), for stipe length (3.31cm ± 0.01 - 7.41cm ± 0.02) while the fresh fruit body weight (115.2g ± 2.01 - 175.3g ± 2.01). It is observed that the growing of P. ostreatus using wastes will help to reduce the huge agricultural wastes within our environment that sometimes when not properly managed caused serious environmental pollution. The research therefore recommended for the use of sawdust in the growing of Pleurotus ostreatus mushroom species, since sawdust and mushroom are good remediators of crude oil pollution. The summary of the result indicated that increase in crude oil pollution significantly (P≤0.5) affected the macro morphological features of the oyster mushroom production.Keywords: Oyster mushroom, Pleurotus ostreatus, morphological parameters and crude oil.

scholarly journals Pengaruh Konsentrasi dan Interval Penyiraman Air Kelapa terhadap Pertumbuhan dan Hasil Jamur Tiram Putih (Pleurotus ostreatus)

2020 ◽  
Vol 4 (1) ◽  
pp. 1-12
Author(s):  
Nur Azizah ◽  
Rita Hayati ◽  
Nurhayati Nurhayati
Keyword(s):  
Water Treatment ◽  
Pleurotus Ostreatus ◽  
Day Treatment ◽  
Coconut Water ◽  
Growth And Yield ◽  
Oyster Mushroom ◽  
Initial Growth ◽  
Fresh Weight ◽  
Mycelium Growth ◽  
Unit Unit

Abstrak. Penelitian ini bertujuan untuk mengetahui pengaruh konsentrasi dan interval penyiraman air kelapa yang tepat serta mengetahui pengaruh interaksi antara konsentrasi air kelapa dan interval penyiraman air kelapa terhadap pertumbuhan dan hasil jamur tiram putih. Penelitan ini dilaksanakan di UPTD Balai Benih Hortikultura Saree Kabupaten Aceh Besar, pada bulan mei sampai juli 2018. Unit-unit penelitian disusun berdasarkan rancangan acak lengkap pola faktorial 4x4 dengan 3 ulangan dan dilanjutkan dengan uji beda nyata jujur taraf 5% pada hasil uji F yang signifikan. Faktor pertama adalah pemberian konsentrasi air kelapa dengan konsentrasi: 0, 25, 50 dan 75% dan faktor kedua adalah interval penyiraman air kelapa dengan interval: 1 hari sekali, 2 hari sekali, 3 hari sekali dan 4 hari sekali. Hasil penelitian menunjukkan bahwa perlakuan konsentrasi air kelapa berpengaruh sangat nyata terhadap parameter pertumbuhan awal miselium,diameter tudung jamur, jumlah tubuh buah, bobot segar tubuh buah per baglog dan bobot segar tubuh buah selama 3 kali panen, berpengaruh tidak nyata pada parameter laju pertumbuhan miselium dan panjang tangkai tudung buah. Konsentrasi terbaik terdapat pada perlakuan konsentrasi air kelapa 50%. Interval penyiraman air kelapa berpengaruh sangat nyata pada parameter diameter  tudung jamur, jumlah tubuh buah, bobot segar tubuh buah per baglog dan bobot segar tubuh buah selama 3 kali panen, berpengaruh nyata pada parameter panjang tangkai tudung buah dan berpengaruh tidak nyata pada parameter  pertumbuhan awal miselium, laju pertumbuhan miselium dan jumlah tubuh buah. Interval penyiraman  terbaik terdapat pada perlakuan interval penyiraman 2 hari sekali. Interaksi antara konsentrasi air kelapa dan interval penyiraman berpengaruh nyata terhadap diameter tudung buah. Kombinasi perlakuan terbaik terdapat pada konsentrasi air kelapa 50% dan interval penyiraman 2 kali sehari.The Effect of Concentrations And The Coconut Watering Intervals on The Growth And Yields of White Oyster Mushroom (Pleurotus ostreatus)Abstract. This research aims to determine the effect of concentrations and the intervals of watering of coconut water on the growth and yields of white oyster mushroom. This research was carried out at Saree UPTD Horticulture Seed Centre, Aceh Besar regency from Mei to July 2018. The research units were compiled based on a Completely Randomized Design-factorial  4x4 with 3 replications and continued with Honestly Significance Difference test (Turkey test) level of 5% on a significant F test result. The first factors were the concentrations of coconut water  : 0, 25, 50, and 75 %. The second factors were the interval of watering: once a day, twice a day, three times a day and four times a day. The result of the research showed that the concentrations of coconut water treatment were highly significant on initial growth of mycelium,  mushroom hood diameter, the number of mushrooms, mushroom fresh weight per baglog and mushroom fresh weight for 3 times harvest and it was not significant on the rate of mycelium growth and long fruit hood stalk. 50% concentration of coconut water treatment gives the best result on the growth and yield. The intervals of watering  of coconut water was highly significant on mushroom hood diameter, the number of mushrooms, mushroom fresh weight per baglog and mushroom fresh weight for 3 times harvest and they significant on long fruit hood stalk and not significant on initial growth of mycelium, the rate of mycelium growth and the number of mushrooms. The interval of watering twice a day treatment shows the best result on the growth and yields of white oyster mushroom. The interaction of concentrations of coconut water and the interval of watering was significant on mushroom hood diameter. 50% concentration of coconut water and the interval of watering twice a day shows the best result on growth and yields of white oyster mushroom.

scholarly journals PENGARUH MEDIA TANAM SABUT KELAPA DAN SERBUK KAYU JATI TERHADAP PERTUMBUHAN MISELIUM JAMUR TIRAM PUTIH (PLEUROTUS OSTREATUS)

2021 ◽  
Vol 9 (2) ◽  
pp. 49-60
Author(s):  
Ahmad Yani ◽  
◽  
Nurul Mutmainnah ◽  
Haerunnisa Haerunnisa
Keyword(s):  
Data Analysis ◽  
Pleurotus Ostreatus ◽  
Oyster Mushroom ◽  
Initial Time ◽  
Wood Powder ◽  
Coconut Fiber ◽  
Mycelium Growth ◽  
Coconut Husk ◽  
Teak Wood

The purpose of this study to determine the effect of planting media of coconut husk and teak sawdust on the growth of white oyster mushroom mycelium (Pleurotus ostreatus) and its implementation on fungi class X SMA. The data analysis used was ANOVA and BNJ tests. This study used 4 treatments, namely P0 as much as 1000 grams of bran, P1 as much as 700 grams of teak wood powder + 300 grams of bran, P2 as much as 700 grams of coconut fiber + 300 grams of bran, P3 as much as 350 grams of coconut husk + 350 grams of teak wood powder + 300 grams bran. The results showed that the planting media of coconut husk and teak sawdust on the growth of the white oyster mushroom mycelium (Pleurotus ostreatus) did not significantly affect the initial time of growth with the fastest mycelium growth in Treatment P3 which is day 4 HSI, no significant effect on the length of the mycelium with an average of 19.5 cm in the P3 treatment and significantly affected the mycelium fulfillment time in the P1 treatment, which is day 49 HSI.

scholarly journals Wood decomposition activity of oyster mushroom (Pleurotus ostreatus) isolate in situ

2013 ◽  
Vol 59 (No. 1) ◽  
pp. 28-33 ◽  
Author(s):  
M. Pavlík ◽  
Š. Pavlík
Keyword(s):  
Pleurotus Ostreatus ◽  
Fruit Body ◽  
Mineral Elements ◽  
Mean Value ◽  
Ash Content ◽  
Oyster Mushroom ◽  
Biological Efficiency ◽  
Wood Decomposition ◽  
Body Formation

&nbsp;The purpose of the research was to examine the decomposition rate of beech (Fagus sylvatica) and aspen (Populus tremula) logs after an oyster mushroom (Pleurotus ostreatus) infection as well as the effect of fruit body formation on the chemical composition of wood. The highest mean value of biological efficiency (B.E.) was found on beech logs (29.05%), but relatively high values of B.E. were recorded also on aspen logs (21.69%). The average content of N was about 37% higher in inoculated logs than in the control (not inoculated) ones. The ash content was about 90% higher in inoculated logs. The content of the mineral elements Ca, Mn, Zn, Cu, Pb, Al, B, and Mg had an increasing tendency in inoculated logs compared with control ones. The logs with minimal production of fruit bodies had the highest content of the mineral elements Pb, Al, Fe, Mg and P.

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