scholarly journals USE OF ZPT AND PLANTING MEDIA ON THE GROWTH OF MYCELIUM OYSTER MUSHROOM (Pleurotus ostreatus)

2020 ◽  
Vol 7 (1) ◽  
pp. 2125-2131
Author(s):  
Joshua H.L. Tobing ◽  
Donn Ricky ◽  
Meyria K Situmeang
Keyword(s):  
Acetic Acid ◽  
Pleurotus Ostreatus ◽  
Water Extract ◽  
Boiling Water ◽  
Oyster Mushroom ◽  
Naphthalene Acetic Acid ◽  
Duncan Multiple Range Test ◽  
Green Beans ◽  
Mycelium Growth ◽  
Range Test

White Oyster mushroom (Pleurotus ostreatus) is an alternative food for the society because of its high nutrients content. This study uses of ZPT and media to see the grow of the mycelium white oyster mushroom. Media used are the boiling water extract of nuts (green beans, soy bean, peanuts, beans of string bean, and beans of nut snaps) and ZPT (Naphthalene Acetic Acid and Kinetin). Anova was used to analyze the data at a significant level of α = 0.05. The results shows that: (1) There’s a significant effect of ZPT and Non-ZPT on the mycelium growth with p=0.000, Duncan Multiple Range Test (DMRT) shows that Kinetin is highest contribution to the significancy of Anova; (2) planting media used in the study shows a significant differences on the mycelium growth with p=0039, Duncan Multiple Range Test shows that beans of long bean and soy bean are the highest contribution to the significancy of Anova; (3) time/days of observation  done in the study shows a significant differences on the mycelium growth with p=0000, Duncan Multiple Range Test shows that T6 or day 14 of observation shows the highest contribution to the significancy of Anova; (4) the interaction of ZPT, Non-ZPT and planting media show a significant differences on the mycelium growth p=0000; (5) the interaction of ZPT, Non-ZPT and time show a significant differences on the mycelium growth with p=0000; (6) the interaction of media and planting time show a significant differences on the mycelium growth with p=0000; and (7) the interaction of ZPT, Non-ZPT and planting media and time do not affect significantly the mycelium growth with  p=0053.

scholarly journals Pertumbuhan Lili (Lilium longiflorum) Pada Berbagai Komposisi Media Tanam dan Zat Pengatur Tumbuh Naphthalene Acetic Acid (NAA) pada Tahap Aklimatisasi

2019 ◽  
Vol 4 (2) ◽  
pp. 144-151
Author(s):  
Lulu Fatikhatul Maryamah ◽  
Florentina Kusmiyati ◽  
Syaiful Anwar
Keyword(s):  
Acetic Acid ◽  
Lilium Longiflorum ◽  
Naphthalene Acetic Acid ◽  
Duncan Multiple Range Test ◽  
Range Test

Zat Pengatur Tumbuh (ZPT) merupakan senyawa organik yang dapat mengatur proses fisiologi tanaman. Penelitian ini bertujuan untuk mengkaji pengaruh komposisi media dan konsentrasi NAA serta kombinasi media dan NAA terhadap pertumbuhan lili pada tahap aklimatisasi. Penelitian dilakukan di rumah plastik di kecamatan Tembalang dan Laboratorium Fisiologi dan Pemuliaan Tanaman, Fakultas Peternakan dan Pertanian Universitas Diponegoro, Semarang. Rancangan yang digunakan dalam penelitian adalah Rancangan Acak Lengkap (RAL) Faktorial 3 x 5 dengan ulangan 3 kali. Faktor pertama adalah berbagai komposisi media tanam yaitu arang sekam dan kompos, yang terdiri dari M1 (1 : 1), M2 (2 : 1) dan M3 (3 : 1). Faktor kedua adalah konsentrasi NAA terdiri dari D0 (0 ppm), D1 (2,5 ppm), D2 (5 ppm), D3 (7,5 ppm), D4 (10 ppm). Analisis data menggunakan uji anova dengan taraf signifikan 5%, jika ada perbedaan akan diuji lanjut dengan uji Duncan Multiple Range-Test (DMRT) pada taraf α = 5%.  Hasil penelitian menunjukkan berbagai komposisi media menghasilkan umur bertunas, tinggi tanaman, panjang akar dan bobot segar yang berbeda nyata, tapi jumlah akar yang tidak berbeda nyata. Komposisi terbaik didapatkan pada arang sekam dan kompos pada perbandingan 3:1. Konsentrasi NAA menghasilkan umur bertunas, tinggi tanaman, jumlah daun, panjang akar dan bobot segar yang tidak berbeda nyata. Kata kunci : lili, komposisi media, konsentrasi dan NAA

scholarly journals Pengaruh penambahan serbuk jagung pada komposisi media tanam terhadap pertumbuhan miselium bibit F1 jamur tiram putih (Pleurotus ostreatus)

2021 ◽  
Vol 10 (2) ◽  
pp. 158-170
Author(s):  
Muhaeming Muhaeming ◽  
Jamilah Jamilah ◽  
Zulkarnaim Zulkarnaim
Keyword(s):  
Experimental Study ◽  
Analysis Of Variance ◽  
Pleurotus Ostreatus ◽  
Medium Composition ◽  
Oyster Mushroom ◽  
Control Treatment ◽  
Mycelium Growth ◽  
Randomized Design ◽  
Significant Difference ◽  
Completely Randomized Design

Penambahan nutrisi perlu dilakukan untuk meningkatkan produktivitas pertumbuhan jamur tiram putih (Pleurotus ostreatus). Salah satu bahan yang dapat digunakan adalah serbuk jagung. Penelitian ini bertujuan untuk mengetahui pengaruh penambahan serbuk jagung pada komposisi media tanam terhadap pertumbuhan miselium bibit F1 jamur tiram putih (Pleurotus ostreatus) dan mengetahui konsentrasi serbuk jagung yang paling baik digunakan pada pertumbuhan  miselium bibit F1 jamur tiram putih (Pleurotus ostreatus). Penelitian ini mengunakan Rancangan Acak Lengkap (RAL) 4 perlakuan dan 3 ulangan sehingga diperoleh 12 satuan percobaan; P0 (perlakuan kontrol), P1 (25%), P2 (50%), dan P3 (100%). Variabel yang diukur adalah persentase pertumbuhan miselium yang diamati pada hari ke-6, 12 dan 18 dan waktu penyebaran miselium Hari Setelah Inokulasi (HSI). Data hasil penelitian dianalisis menggunakan analysis of variance (ANOVA) dengan uji lanjut menggunakan uji Beda Nyata Terkecil (BNT). Hasil penelitian menunjukkan bahwa ada pengaruh pada pertumbuhan miselium bibit F1 jamur tiram putih (Pleurotus ostreatus) dengan penambahan serbuk jagung pada komposisi media tanam. Selain Itu, konsentrasi serbuk jagung yang memberikan hasil yang paling baik terhadap pertumbuhan miselium adalah P2 dengan konsentrasi 50%. Kata kunci : miselium F1; Pleurotus ostreatus; serbuk jagung  ABSTRACTAdditional nutrition needs to be done to increase the productivity of oyster mushroom (Pleurotus ostreatus) growth. One of the materials that can be used is corn powder. This study aimed to determine the effect of corn powder on the planting medium composition towards mycelium growth of oyster mushroom F1 seeds (Pleurotus ostreatus) and to find the best concentration of corn powder to grow mycelium seeds F1 oyster mushroom (Pleurotus ostreatus). This research was an experimental study using a Completely Randomized Design (CRD) with 4 treatments namely; P0 (control treatment), P1 (25%), P2 (50%), and P3 (100%), and 3 repetitions so that obtained 12 experimental units. The variables measured were the mycelium growth percentage on days 6, 12, and 18, and the mycelium widespread time after the day of inoculation. The data were analyzed using ANOVA then continued on the Least Significant Difference (LSD) test. The results showed that the addition of corn powder on oyster mushroom growing media affected mycelium growth of the oyster mushroom F1 seeds (Pleurotus ostreatus). It also found that the P2 with a 50% concentration of corn powder gave the widest mycelium growth.  Keywords: corn powder; mycelium; Pleurotus ostreatus

The Growth Of White Oyster Mushroom Mycelium (Pleurotus ostreatus) (Jacq) (P. Kumm) From Liquid And Solid Inoculum

Biocelebes ◽  
2019 ◽  
Vol 13 (3) ◽  
pp. 279-287
Author(s):  
Nisfaun Safitriana ◽  
Umrah ◽  
Orryani Lambui
Keyword(s):  
Liquid Medium ◽  
Incubation Time ◽  
Pleurotus Ostreatus ◽  
Solid Medium ◽  
Oyster Mushroom ◽  
Mycelium Growth ◽  
Average Growth ◽  
Solid Media ◽  
Randomized Design ◽  
Department Faculty

The study about  the growth of white oyster mushroom mycelium (Pleurotus ostreatus) (Jacq) (P. Kumm) from liquid and solid inoculum conducted on January until July 2018 in Biotechnology laboratory, Biology Department, Faculty of Math and Science, Tadulako University. This study was aimed to find out the mycelium growth of source of stem inoculum and hood in liquid and solid media. This study was designed by Complete Randomized Design (CRD) which consisted of four treatments and five replications. The treatment were M1 (source of stem inoculum in liquid medium), M2 (source of stem inoculum in solid medium), M3 (source of hood inoculum in liquid medium), and M4 (source of hood inoculum in solid medium). The parameters were (a) incubation time till mycelium fills medium, (b) CFU, and (c) the viability of inoculum on producing medium. The result showed that the faster incubation were M1 and M3 (for 2 days, higher CFU was M3 with an average number 8,2 x 10 10 CFU /ml. The faster viability of mycelium growth were M3 with an average growth of mycelium 6,97 cm/days.

Resource Utilization Residue of Pleurotus ostreatus

2013 ◽  
Vol 807-809 ◽  
pp. 2048-2053
Author(s):  
Yong Xiong ◽  
Chun Yan Zhao ◽  
Yi Jian Chen ◽  
Wan Song Yue
Keyword(s):  
Pleurotus Ostreatus ◽  
Ganoderma Lucidum ◽  
Water Extract ◽  
Ethanol Extract ◽  
Flammulina Velutipes ◽  
Edible Fungi ◽  
Mycelium Growth ◽  
Extract Concentration ◽  
Ethanol Extracts ◽  
Better Than

The allelopathy of spent substrate extracts including water extract and ethanol extract ofPleurotus ostreatuson the mycelium growth of six edible fungi, i.e.Flammulina velutipes,Ganoderma lucidum Karst,Pleurotus ostreatusandCordyceps (three species), were investigated using Petri dishes approach. The results indicated that the spent substrate extracts have different effects on their mycelium growth. The mycelium ofFlammulina velutipesgrows better than control check with increasing water extract concentration. The mycelia ofGanoderma lucidum Karstgrow first promotion after inhibition with increasing water extract concentration. The mycelia ofFlammulina velutipes, Ganoderma lucidum Karst, Pleurotus ostreatusare promoted by ethanol extracts. The mycelia of Cordyceps (three species) grow first promotion after inhibition with ethanol extracts. The results can provide reference values for rational utilization of the spent mushroom substrate.

scholarly journals PENGARUH PENGGUNAAN ASAM ASAM ASETAT, ASAM KLORIDA DAN ASAM SITRAT TERHADAP KARAKTERISTIK LEM DARI LIMBAH SISIK IKAN KAKAP MERAH (Lutjanus argentimaculatus)

2020 ◽  
Vol 3 (2) ◽  
Author(s):  
Mariani Mariani ◽  
Haslianti Haslianti ◽  
Suwarjoyowirayatno Suwarjoyowirayatno
Keyword(s):  
Acetic Acid ◽  
Citric Acid ◽  
Hydrochloric Acid ◽  
Water Content ◽  
Wood Surface ◽  
Surface Damage ◽  
Red Snapper ◽  
Fish Scales ◽  
Duncan Multiple Range Test ◽  
Range Test

ABSTRACT         The aim of this study was to determine the effect of acetic acid, hydrochloric acid, and citric acid on the physical characteristics of glue from red snapper scales. This study used a completely randomized design (CRD) with one factor of the acid solvent type (5% acetic acid, 5% hydrochloric acid, and 5% citric acid with 150 g of fish scales. Observation data were analyzed using ANOVA (Analysis of Variance) at the 95% level, if there is a significant difference (p <0.05) then a further test is carried out with the DMRT (Duncan Multiple Range Test) tests at the 95% significant level. The results obtained showed that the effect of acetic acid, hydrochloric acid, and citric acid on the characteristics of glue from red snapper fish scales had a significant effect on the adhesive strength value of fish glue, wood surface damage, viscosity, water content but not the pH of fish glue. The best results for the stickiness of fish glue, wood surface damage, fish glue viscosity, pH, and water content were obtained on extraction using acetic acid 6.61; 40.75; 3.60; 4.46; and 48,26 respectively. The results obtained showed that the effect of using acetic acid, hydrochloric acid, and citric acid on the characteristics of glue from red snapper fish scales had a significant effect on the adhesive strength value of fish glue, wood surface damage, viscosity, water content but not the pH of fish glue. Keywords: Acetic acid, citric acid,  fish glue, fish scales, hydrochloric acid.ABSTRAKPenelitian ini bertujuan untuk mengetahui pengaruh penambahan asam asetat, asam klorida, dan asam sitrat terhadap karakteristik fisik lem dari sisik ikan kakap merah dan untuk mengetahui pengaruh penambahan asam asetat, asam klorida dan asam sitrat terhadap karakteristik kimia lem dari sisik ikan kakap merah. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan satu faktor yaitu jenis pelarut asam (asam asetat 5%, asam klorida 5%, dan asam sitrat 5% dengan sisik ikan sebanyak 150 g. Data hasil pengamatan dianalisa menggunakan ANOVA (Analysis of Variance) pada taraf 95%, apabila terdapat beda nyata (p<0,05) maka dilakukan uji lanjut dengan uji DMRT (Duncan Multiple Range Test) pada taraf nyata 95%. Hasil penelitian yang diperoleh menunjukkan bahwa pengaruh penggunaan asam asetat, asam klorida dan asam sitrat terhadap karakteristik lem dari limbah sisik ikan kakap merah memberikan pengaruh nyata terhadap nilai keteguhan rekat lem ikan, kerusakan permukaan kayu, viskositas, kadar air namun tidak dengan pH lem ikan. Hasil terbaik untuk uji keteguhan rekat lem ikan, kerusakan permukaan kayu, viskositas, pH lem ikan dan kadar air lem ikan terdapat pada ekstraksi menggunakan asam asetat dengan nilai masing-masing 6.61; 40.75; 3.60; 4.46 dan 48.26.Kata kunci: Asam asetat, asam klorida, asam sitrat, lem ikan, sisik ikan

scholarly journals Pengaruh Konsentrasi dan Interval Penyiraman Air Kelapa terhadap Pertumbuhan dan Hasil Jamur Tiram Putih (Pleurotus ostreatus)

2020 ◽  
Vol 4 (1) ◽  
pp. 1-12
Author(s):  
Nur Azizah ◽  
Rita Hayati ◽  
Nurhayati Nurhayati
Keyword(s):  
Water Treatment ◽  
Pleurotus Ostreatus ◽  
Day Treatment ◽  
Coconut Water ◽  
Growth And Yield ◽  
Oyster Mushroom ◽  
Initial Growth ◽  
Fresh Weight ◽  
Mycelium Growth ◽  
Unit Unit

Abstrak. Penelitian ini bertujuan untuk mengetahui pengaruh konsentrasi dan interval penyiraman air kelapa yang tepat serta mengetahui pengaruh interaksi antara konsentrasi air kelapa dan interval penyiraman air kelapa terhadap pertumbuhan dan hasil jamur tiram putih. Penelitan ini dilaksanakan di UPTD Balai Benih Hortikultura Saree Kabupaten Aceh Besar, pada bulan mei sampai juli 2018. Unit-unit penelitian disusun berdasarkan rancangan acak lengkap pola faktorial 4x4 dengan 3 ulangan dan dilanjutkan dengan uji beda nyata jujur taraf 5% pada hasil uji F yang signifikan. Faktor pertama adalah pemberian konsentrasi air kelapa dengan konsentrasi: 0, 25, 50 dan 75% dan faktor kedua adalah interval penyiraman air kelapa dengan interval: 1 hari sekali, 2 hari sekali, 3 hari sekali dan 4 hari sekali. Hasil penelitian menunjukkan bahwa perlakuan konsentrasi air kelapa berpengaruh sangat nyata terhadap parameter pertumbuhan awal miselium,diameter tudung jamur, jumlah tubuh buah, bobot segar tubuh buah per baglog dan bobot segar tubuh buah selama 3 kali panen, berpengaruh tidak nyata pada parameter laju pertumbuhan miselium dan panjang tangkai tudung buah. Konsentrasi terbaik terdapat pada perlakuan konsentrasi air kelapa 50%. Interval penyiraman air kelapa berpengaruh sangat nyata pada parameter diameter  tudung jamur, jumlah tubuh buah, bobot segar tubuh buah per baglog dan bobot segar tubuh buah selama 3 kali panen, berpengaruh nyata pada parameter panjang tangkai tudung buah dan berpengaruh tidak nyata pada parameter  pertumbuhan awal miselium, laju pertumbuhan miselium dan jumlah tubuh buah. Interval penyiraman  terbaik terdapat pada perlakuan interval penyiraman 2 hari sekali. Interaksi antara konsentrasi air kelapa dan interval penyiraman berpengaruh nyata terhadap diameter tudung buah. Kombinasi perlakuan terbaik terdapat pada konsentrasi air kelapa 50% dan interval penyiraman 2 kali sehari.The Effect of Concentrations And The Coconut Watering Intervals on The Growth And Yields of White Oyster Mushroom (Pleurotus ostreatus)Abstract. This research aims to determine the effect of concentrations and the intervals of watering of coconut water on the growth and yields of white oyster mushroom. This research was carried out at Saree UPTD Horticulture Seed Centre, Aceh Besar regency from Mei to July 2018. The research units were compiled based on a Completely Randomized Design-factorial  4x4 with 3 replications and continued with Honestly Significance Difference test (Turkey test) level of 5% on a significant F test result. The first factors were the concentrations of coconut water  : 0, 25, 50, and 75 %. The second factors were the interval of watering: once a day, twice a day, three times a day and four times a day. The result of the research showed that the concentrations of coconut water treatment were highly significant on initial growth of mycelium,  mushroom hood diameter, the number of mushrooms, mushroom fresh weight per baglog and mushroom fresh weight for 3 times harvest and it was not significant on the rate of mycelium growth and long fruit hood stalk. 50% concentration of coconut water treatment gives the best result on the growth and yield. The intervals of watering  of coconut water was highly significant on mushroom hood diameter, the number of mushrooms, mushroom fresh weight per baglog and mushroom fresh weight for 3 times harvest and they significant on long fruit hood stalk and not significant on initial growth of mycelium, the rate of mycelium growth and the number of mushrooms. The interval of watering twice a day treatment shows the best result on the growth and yields of white oyster mushroom. The interaction of concentrations of coconut water and the interval of watering was significant on mushroom hood diameter. 50% concentration of coconut water and the interval of watering twice a day shows the best result on growth and yields of white oyster mushroom.

scholarly journals PENGARUH MEDIA TANAM SABUT KELAPA DAN SERBUK KAYU JATI TERHADAP PERTUMBUHAN MISELIUM JAMUR TIRAM PUTIH (PLEUROTUS OSTREATUS)

2021 ◽  
Vol 9 (2) ◽  
pp. 49-60
Author(s):  
Ahmad Yani ◽  
◽  
Nurul Mutmainnah ◽  
Haerunnisa Haerunnisa
Keyword(s):  
Data Analysis ◽  
Pleurotus Ostreatus ◽  
Oyster Mushroom ◽  
Initial Time ◽  
Wood Powder ◽  
Coconut Fiber ◽  
Mycelium Growth ◽  
Coconut Husk ◽  
Teak Wood

The purpose of this study to determine the effect of planting media of coconut husk and teak sawdust on the growth of white oyster mushroom mycelium (Pleurotus ostreatus) and its implementation on fungi class X SMA. The data analysis used was ANOVA and BNJ tests. This study used 4 treatments, namely P0 as much as 1000 grams of bran, P1 as much as 700 grams of teak wood powder + 300 grams of bran, P2 as much as 700 grams of coconut fiber + 300 grams of bran, P3 as much as 350 grams of coconut husk + 350 grams of teak wood powder + 300 grams bran. The results showed that the planting media of coconut husk and teak sawdust on the growth of the white oyster mushroom mycelium (Pleurotus ostreatus) did not significantly affect the initial time of growth with the fastest mycelium growth in Treatment P3 which is day 4 HSI, no significant effect on the length of the mycelium with an average of 19.5 cm in the P3 treatment and significantly affected the mycelium fulfillment time in the P1 treatment, which is day 49 HSI.

scholarly journals Pemanfaatan Limbah Serbuk Teh sebagai Substitusi Serbuk Gergaji terhadap Pertumbuhan Miselium dan Produksi Jamur Tiram Putih (Pleurotus Ostreatus)

2018 ◽  
Vol 3 (1) ◽  
pp. 27
Author(s):  
Siti Mardiana ◽  
Ellen L Panggabean ◽  
Retno A Kuswardani ◽  
Muhammad Usman
Keyword(s):  
Fruiting Body ◽  
Pleurotus Ostreatus ◽  
Fruit Body ◽  
Oyster Mushroom ◽  
Combined Treatments ◽  
Mycelium Growth ◽  
Growing Media ◽  
Randomized Design ◽  
Tea Waste ◽  
Completely Randomized Design

<p class="HowToCite"><em>The waste of tea powder is abundant and has not been widely used optimally. The alternative use is to use the waste as an additional medium or substitution for the white oyster mushroom growing media. The purpose of the study was to determine the effect of mycelium growth and the production of the fruit of the white oyster mushroom on combination media / tea powder substitution with sawdust. The research design used was a completely randomized design (CRD) with 4 replications. Combined treatments were M0 = 100% sawdust; M1 = 100% Tea waste powder; M2 = 75% tea powder + 25% sawdust; M3 = 50% tea powder + 50% sawdust and M4 = 25% tea powder + 75% sawdust. All treatments used the composition of the manufacture of mushroom growing media consisting of each combination of treatments, bran 10% by weight of the material and lime 0.5% by weight of the material. The parameters observed were the growth of white oyster mushroom mycelium, the rate of mycelium growth, the time the mycelium closed the substrate, the time of appearance of the fruiting body, the weight of the fruit body and the diameter of the fruit hood. The results showed that all parameters differed significantly. The best treatment is in treatment M4, followed by treatment of M3, M0 and M2.</em></p>

Morphological and Molecular Identification of Oyster Mushroom [Pleurotus Ostreatus (Jacq.) P. Kumm]

2021 ◽  
Author(s):  
Urvashi Kotadiya ◽  
Jayadipkmuar Talaviya ◽  
Kalpit Shah ◽  
Sandip Lathiya
Keyword(s):  
Pleurotus Ostreatus ◽  
Smooth Surface ◽  
Gene Bank ◽  
Oyster Mushroom ◽  
Its Sequencing ◽  
Accession Number ◽  
Edible Fungi ◽  
Mycelium Growth ◽  
The Third ◽  
The World

Abstract Oyster mushroom (Pleurotus ostreatus) is fleshy, edible fungi and used throughout the world. Among the most well-known species of mushrooms, Pleurotus sp. is the third most popularly grown mushroom in the world and rank second in India. The most well-known species of Pleurotus are P. ostreatus, P. florida, P. eryngii, P. cystidiosis, P. flabellatus, P. cornucopie, and P. sajor-caju. According to the morphological, molecular character and ITS sequencing, the Pleurotus ostreatus was distinguish from the other species. The cap (pileus) of the oyster mushroom has whitish to grey color and fleshy. Stipe cream and smooth surface. Spores are whitish to lilac grey in mass, cylindrical to oblong shape. Spore print was white in color. Whitish mycelium growth in PDA plate. The ITS sequence was submitted to gene bank NCBI and it was accepted by NCBI with accession number MW446165.

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