scholarly journals POTENTIAL MICROALGA Chlorella vulgaris FOR BIOREMEDIATION OF HEAVY METAL Pb

2020 ◽  
Vol 2 (3) ◽  
pp. 224-234
Author(s):  
Asyatul Halima ◽  
Nursyirwani Nursyirwani ◽  
Irwan Effendi ◽  
Hanies Ambarsar

This research was conducted from April to July 2019 at the Center for Environmental Technology Laboratory (PTL), Geostech 820 Building, Serpong Region Puspitek, South Tangerang. The aim of this research was to determin growth of Chlorella vulgaris on media added with Pb at different concentrations, and to determine the ability to absorb Pb. The experimental method was conducted by using concentrations of Pb at 3 different levels consisting of 1 ppm, 5 ppm, 10 ppm in triplicate and control treatment without the addition of Pb. Each sample was analyzed by ICP-OES (Inductivly Coupled Plasma – Optical Emission Spectrometer). Data was analyzed by using ANOVA followed by LSD test. The growth of C. vulgaris biomass during the cultivation were Pb 1 ppm (10.38 g / l), k (9.10 g / l), Pb 5 ppm (8.36 g/l) and Pb 10 ppm (7.13) g/l). ANOVA test showed that different concentrations of Pb gave a very significant difference (Sig. <0.05) on the growth of C. vulgaris. Reduction in the concentration of Pb metal in culture media were Pb 10 ppm (96.8%), Pb 5 ppm (96.2%), Pb 1 ppm (90%) and there is no Pb found in control. ANOVA test results showed that C. vulgaris had a very significant effect (Sig. <0.05) on the decrease of Pb metal concentration. This shows that C. vulgaris has the capacity as bioremediation of Pb with different concentrations.

2021 ◽  
Vol 4 (1) ◽  
pp. 6
Author(s):  
Alivy Aulia Az Zahra ◽  
Natasha Winona Audrey ◽  
Devalna Siwi Ichyana ◽  
Tania Saskianti ◽  
Seno Pradopo ◽  
...  

Background: Down Syndrome children have better ability in capturing instructions visually. The audio-visual learning method can be applied to Down Syndrome children. Electric toothbrushes were made to make it easier for them to brush their teeth, besides those electric toothbrushes have a handle that is comfortable to hold and control. Purpose: The purpose of this study is to determine the effectiveness of electric and manual toothbrushes on Down Syndrome children OHI-S scores and determine the effect of education on tooth brushing with animated videos on Down Syndrome children OHI-S scores. Methods: Subjects of the study were 34 Down Syndrome children aged 6-19 years in Surabaya, East Java, Indonesia. OHI-S scores were recorded before and after the subjects were educated to brush their teeth with animated videos or dental phantoms. OHI-S scores were also recorded before and after the subjects brushed their teeth using manual toothbrushes or electric toothbrushes. Results: The test results Independent T-test (p> 0.05) showed there was no significant difference between tooth brushing using a manual toothbrush and an electric toothbrush to the decline of OHI-S score of Down Syndrome children. The results of the Mann-Whitney statistical test were p> 0.05, that there was no significant difference in the reduction in OHI-S scores between Down Syndrome children who were educated with animated videos and dental phantoms. Conclusion: There was no significant difference between manual toothbrushes and electric toothbrushes and there was no significant influence of Down Syndrome OHI-S children who were educated with animated videos or dental phantoms.


Author(s):  
Cenikli U ◽  
◽  
Bir LS ◽  
Degirmenci E ◽  
Ardıç FN ◽  
...  

Background: Essential Tremor (ET) is the most common movement disorder, yet the location of the primary disease substrate continues to be a matter of debate. In this study, we aimed to evaluate ocular movement abnormalities with Electrooculography (EOG) in patients with ET to find a possible location of disease pathology. Methods: Electrooculographic evaluation including saccade, tracking, optokinetic, gaze and positional tests were performed to 36 ET patients and 36 healthy subjects. Patient age on the onset of the tremor, duration of the disease, characteristics and the location of the tremor were also investigated. Fahn- Tolosa-Marin tremor rating scale was used to determine the tremor severity. Differences of abnormal test results between patient and control groups were analysed with Pearson’s and Fisher’s Exact and correlation analyses of EOG tests and clinical data were performed with Spearman’s and Pearson’s correlation tests. Results: There was not any significant difference in EOG tests between the ET patients and controls. Significant correlation was only found between EOG abnormality and patient age in correlation analyses. Conclusions: Our results showed that ET patients may not have specific EOG test abnormalities. These tests would be used especially in the different diagnosis of other movement disorders.


Zygote ◽  
2020 ◽  
Vol 28 (6) ◽  
pp. 447-452
Author(s):  
Seungbum Hong ◽  
Binoy S. Vettical ◽  
Nisar Ahmad Wani

SummaryExperiments were conducted to study in vitro maturation of prepubertal goat oocytes and their developmental potential after chemical activation. In Experiment 1, cumulus–oocytes complexes collected from the ovaries of prepubertal goats slaughtered at a local abattoir were matured in vitro in TCM-199-based medium supplemented with 10 µg/ml luteinizing hormone (LH) (treatment 1) or 10 µg/ml LH + 0.1 mM l-cysteine (treatment 2). In Experiment 2, mature oocytes were activated with either 5 µM ionomycin or 7% ethanol. After 18 h, some oocytes were randomly fixed and stained to evaluate their chromatin status, while others were cultured in embryo culture medium to study their further development. In Experiment 3, oocytes activated with 5 µM ionomycin were cultured for 7 days in one of the four different culture media [Charles Rosenkrans medium (CR-1), TCM-199, potassium simplex optimization medium (KSOM) and synthetic oviductal fluid (SOF)] to study their developmental potential. The maturation rate in control, treatment 1, and treatment 2 media did not differ from each other (P > 0.05). However, the lowest degeneration of oocytes was observed in treatment 3 (P < 0.05) when compared with the other two groups. The proportion of activated oocytes was higher, while non-activated oocytes were lower in ionomycin group when compared with the group activated with ethanol (P < 0.05). The proportions of oocytes cleaved were 65.7, 56.8, 61.0 and 54.4% in CR-1, TCM-199, KSOM and SOF medium, respectively, with no significant difference. However, further development of cleaved oocytes was better in KSOM followed by SOF.


2020 ◽  
Author(s):  
Domina Delač ◽  
Paulo Pereira ◽  
Ivica Kisić

&lt;p&gt;&lt;strong&gt;The effects of mulch (&lt;em&gt;Olea europea&lt;/em&gt; and &lt;em&gt;Pinus halepensis&lt;/em&gt;) on burned soils: A preliminary study in Adriatic coast (Croatia)&lt;/strong&gt;&lt;/p&gt;&lt;p&gt;Delac, Domina&lt;sup&gt;1*&lt;/sup&gt;; Pereira, Paulo&lt;sup&gt;2*&lt;/sup&gt;; Kisic, Ivica&lt;sup&gt;1&lt;/sup&gt;&lt;/p&gt;&lt;p&gt;&lt;sup&gt;1&lt;/sup&gt;University of Zagreb, Faculty of Agriculture, Department of General Agronomy, Svetosimunska cesta 25, 10000 Zagreb, Croatia. (*[email protected])&lt;/p&gt;&lt;p&gt;&lt;sup&gt;2&lt;/sup&gt;Mykolas Romeris University, Environmental Management Laboratory, Ateitis street 20, LT-08303, Vilnius, Lithuania (*[email protected])&lt;/p&gt;&lt;p&gt;&amp;#160;&lt;/p&gt;&lt;p&gt;In the recent decades the frequency and intensity of summer drought periods is increasing in Adriatic coast. These changes in climate increase the vulnerability to wildfires. Wildfires can change soil physical and chemical properties. However, these effects can be mitigated by mulching. The aim of this work is to study the effects of mulch (&lt;em&gt;Olea europea&lt;/em&gt; and &lt;em&gt;Pinus halepensis&lt;/em&gt;) on fire affected soils. The wildfire occurred on 28 July 2019 and affected an area of about 900 ha in Dalmatia, near Adriatic Coast (43&amp;#176;45'06.0&quot;N 15&amp;#176;56'02.9&quot;E with an elevation of 105 m a.s.l.). &amp;#160;The mean annual temperature is 15.8 &amp;#176;C, and the annual precipitation is 800 mm. It was affected agricultural land with dominant culture &lt;em&gt;Olea europea&lt;/em&gt; and abandoned grassing where dominates &lt;em&gt;Pinus halepensis&lt;/em&gt;. Soils are classified as &lt;em&gt;calcocambisols&lt;/em&gt;. Twenty-five days after the fire, two plots (5 treatments per plot) were established and covered with &lt;em&gt;Olea europea&lt;/em&gt; and &lt;em&gt;Pinus halepensis&lt;/em&gt; mulch. A control plot was established as well. Soil were sampled (0 &amp;#8211; 5 cm), twenty- days after fire (August, 2019), before mulch application, and then 3 months after fire (November, 2019). A total of 15 samples were collected per treatment (45 each sampling date). The soil properties analysed were soil pH, soil organic matter (SOM), mean weight diameter (MWD) to express aggregate stability, and soil water repellency (SWR) measured with water drop penetration time method (WDPT) in different fractions (2 &amp;#8211; 1 mm; 1 &amp;#8211; 0.5 mm, 0.5 &amp;#8211; 0.25 mm, and &lt;0.25 mm). Soil pH was not significantly different among sampling dates and treatments. SOM was significantly different among sampling dates for &lt;em&gt;Olea europeae&lt;/em&gt; treatment and control. &lt;em&gt;Olea europeae&lt;/em&gt; treatment had a significantly higher SOM then &lt;em&gt;Pinus halepensis&lt;/em&gt; and control treatment. MWD was significantly higher within &lt;em&gt;Olea europeae&lt;/em&gt; treatment. Within &lt;em&gt;Pinus halepensis&lt;/em&gt; and control treatment no significant difference was observed. The soil was classified as slightly water repellent (5 &amp;#8211; 60 seconds) in &lt;em&gt;Olea europeae&lt;/em&gt; soil finer fraction (0.5 &amp;#8211; 0.25 mm and &lt;0.25) in both sampling dates. In &lt;em&gt;Pinus halepensis&lt;/em&gt; treatments and control, soil was wettable (&lt;5 seconds), and no significant difference was observed among sampling date. Future sampling and analysis will be conducted during one year to estimate the effect of &lt;em&gt;Olea europeae&lt;/em&gt; and &lt;em&gt;Pinus halepensis&lt;/em&gt; mulch on soil properties.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Keywords: &lt;/strong&gt;Wildfire, &lt;em&gt;Olea europeae&lt;/em&gt;&lt;strong&gt;, &lt;/strong&gt;&lt;em&gt;Pinus halepensis&lt;/em&gt;, mulch.&lt;/p&gt;&lt;p&gt;&amp;#160;&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Acknowledgments&lt;/strong&gt;&lt;/p&gt;&lt;p&gt;This work was supported by Croatian Science Foundation through the project &quot;Influence of Summer Fire on Soil and Water Quality&amp;#8221; (IP-01-2018-1645).&lt;/p&gt;


2015 ◽  
Vol 27 (2) ◽  
Author(s):  
Mohammad Azrul Izzudin ◽  
Elin Karlina ◽  
Ratna Indriyanti

Introduction: Acidulated phosphate fluoride (APF) gels are commonly used as preventive caries materials in paediatric dentistry while glass ionomer cements (GICs) are widely used as a restorative material. The purpose of this study was to determine the effect of acidulated phosphate fluoride application towards the microhardness of GIC. Methods: This study was an experimental laboratory study where ten specimens were prepared from high viscosity GIC, which was Fuji IX. Specimens were stored in 50 ml distilled water at first 24 hours at 37°C, and then specimens were divided into fluoride group which was immersed in 25 ml of 1.23 % APF gel and control group which was stored 25 ml distilled water for 24 hours at 37°C. The Micromet II Microhardness Tester, Buehler, IL, USA that was standardised for Vickers hardness test was used to test the specimens at 100-gram load. Data were analysed using the t-test comparison test. Results: The level of microhardness of the fluoride group (14.34) was much lower compared to the control group (43.21) with a highly significant difference (p<0.01). Conclusion: The application of 1.23% APF gel on high viscosity GIC reduces the microhardness level compared to the control group.


2018 ◽  
Vol 146 (9-10) ◽  
pp. 492-497 ◽  
Author(s):  
Ljiljana Bjelovic ◽  
Jelena Krunic ◽  
Nikola Stojanovic ◽  
Jelena Eric ◽  
Tatjana Kanjevac

Introduction/Objective. This study was aimed at evaluating dentin permeability after irrigation with sodium hypochlorite (NaOCl) and final rinse with chlorhexidine (CHX), ethylenediamine tetraacetic acid (EDTA) + CHX, and new combination products: QMiX or MTAD. Methods. Roots of 60 maxillary incisors were randomly divided into five groups (n = 12) before instrumentation and irrigation with NaOCl according to the final irrigation regimen: CHX (2% CHX), EDTA + CHX (17% EDTA + 2% CHX), QMiX, MTAD, and control group (distilled water). After final irrigation, ten roots of each group were horizontally sectioned and dye penetration was evaluated in the coronal, middle, and apical thirds. Remaining samples were subjected to scanning electron microscopy. Data were analyzed with ANOVA/Tukey?s test. Results. Less dye penetration was found in CHX group compared with control as well as with QMiX and MTAD group in all thirds (p < 0.05). A significant difference between the control and EDTA + CHX, QMiX or MTAD group was observed only in the apical root third (p < 0.05). Conclusion. Dentin permeability was significantly reduced after final irrigation with CHX, but not after use of other final irrigation solutions, except in the apical third of the root canal.


2016 ◽  
Vol 17 (3) ◽  
pp. 233
Author(s):  
Nanang Kusnandar

<p>Abstrak<br />Salah satu klausul uji dalam SNI kompor gas dengan sistem pemantik ialah pengukuran asupan panas. Dalam makalah ini, eksperimen pengukuran asupan panas pada suatu kompor gas dua tungku dilakukan dengan memvariasikan tekanan input dan lama waktu pengujian untuk melihat pengaruhnya terhadap nilai asupan panas yang dihitung berdasarkan formula dalam SNI 7368:2011 dan SNI 7469:2013. Hasil uji ANOVA pada tingkat keyakinan 95% menunjukkan bahwa terdapat pengaruh yang signifikan dari tekanan input gas pada saat pengujian terhadap asupan panas kompor yang dihasilkan, sedangkan lama waktu pengujian tidak berpengaruh signifikan. Selain itu, dengan uji Mann-Whitney dihasilkan bahwa tidak ada perbedaan signifikan antara nilai asupan panas kompor gas dua tungku yang diperoleh dari penjumlahan nilai asupan panas pada kedua tungku yang diuji secara terpisah dengan hasil dari kedua tungku yang diuji secara bersamaan.<br />Kata Kunci: asupan panas, kompor gas sistem pemantik, SNI 7368:2011, SNI 7469:2013.</p><p><br />Abstract<br />One clause in the SNI of gas stove with lighter system is the measurement of heat input. In this paper, experimental measurements of the heat input on a two-burner gas stove done by varying the input pressure and duration of testing to see its effect on the heat input value calculated based on a formula in SNI 7368: 2011 and SNI 7469: 2013. ANOVA test results at the 95% confidence level indicates that there is significant influence of input pressure to heat input of gas stove obtained, while the duration of the test has no significant effect. Additionally, the Mann-Whitney test result revealed that there is no significant difference between the value of the heat input of two burner gas stove obtained from the sum of the heat input in both burner that is tested separately with the result that is tested simultaneously.<br />Keywords: heat input, gas stove with lighter system, SNI 7368:2011, SNI 7469:2013.</p>


2019 ◽  
Vol 22 (2) ◽  
pp. 103
Author(s):  
Chrisna Adhi Suryono ◽  
Agus Sabdono ◽  
Subagiyo Subagiyo

Organophosphate pesticides were widely used in agriculture and OPP which was less accumulative and degradable but It has been found in an aquatic environment. The purpose of this study was to determine the level of organophosphate pesticide residues in bivalve which was fishing in North Coast of Java specifically the Demak and Surabaya.  Bivalvia, sediment and seawater samples were analysed using GC-MS. The results showed that the bivalves of A inaequivalvis, P viridis, A pectinata captured in Demak and Surabaya were contaminated with organophosphate pesticide of chlorpyrifos, fenitrothion and profenofos. The chlorpyrifos was found in all species of bivalves, but the highest concentrations of OPP were profenophos> chlorpyrifos > fenitrothion respectively. ANOVA test results show that there was a very significant difference in OPP residues in bivalves p = 0.009, but there was no difference in OPP residues between locations.Organoposfat pestisida (OPP) banyak digunakan secara meluas dalam pertanian dan OPP tersebut kurang akumulatif dan mudah terurai namun keberdaanya telah di temukan dala lingkungan perairan. Tujuan dari penelitian adalah untuk mengetahui tingkat akumulasi residu pestisida organoposfat yang terdapat di bivalvia yang ditangkap di pesisir Utara Jawa khususnya wilayah Demak dan Surabaya.  Sampel bivalvia, sedimen dan air laut dianalisa menggunakan GC-MS. Hasil penelitian menunjukan bahwa bivalvia A inaequivalvis, P viridis, A pectinata yang ditangkap di Demak dan Surabaya terkontaminasi pestisida organoposfat jenis Klorpirifos, Fenitrotion dan Profenofos. Klorpirifos ditemukan pada semua bivalvia, namun konsentrasi tertinggi OPP secara berurutan profenofos > klorpirifos > fenitrotion.  Hasil uji ANOVA  menunjukan adanya perbedaan yang sangat nyata residu OPP dalam bivalvia p= 0.009,  namun tidak ada berbedaan residu OPP antar wilayah lokasi.


1970 ◽  
Vol 50 (2) ◽  
pp. 311-318 ◽  
Author(s):  
L. C. SEIER ◽  
R. J. KIRK ◽  
T. J. DEVLIN ◽  
R. J. PARKER

Forty-five male Sapphire mink kits were allotted to three treatment groups and fed for approximately 100 days (August 1–November 7). The control diet (treatment 1) consisted of horse-meat 10%, cooked chicken by-products 15%, raw cereal 25%, and whole frozen fish 50%. The frozen fish was replaced by either herring meal or soybean meal on an equivalent dry matter basis for treatments 2 and 3. Average mink weights on November 7 were 1.78, 1.87, and 1.92 kg for the soybean meal, herring meal, and control treatment groups, respectively, and were not significantly different (P > 0.05). The feeding period was divided into a growing phase (period 1), a growing and furring phase (period 2), and a maintenance and a priming phase (period 3). The average daily weight gains during these periods were: period 1: 15.2, 18.0 and 11.8 g; period 2: 6.1, 6.7 and 7.8 g; period 3: +0.8, −2.6 and −0.7 g, for the control, herring- and soybean meal-fed mink, respectively. Digestibility trials were carried out during each period. Dry matter and energy digestibilities were significantly higher (P < 0.05) for the herring-fed and control kits than for the soybean meal-fed kits. There was no significant difference (P > 0.05) in nitrogen balance among treatments. Skin biopsies were taken at pelting time to compare fur density, with values of 19.6, 20.6, and 19.5 hairs per pore, for the soybean-, herring-fed and control kits, respectively.


2020 ◽  
Vol 19 (1) ◽  
Author(s):  
Favour O. Omeiza ◽  
George O. Ademowo ◽  
Funmilola A. Ayeni

Abstract Background The menace of resistance to anti-malarial drugs is a great challenge to malaria control, necessitating the search for new anti-malarial agents. This search has led to the exploration of natural products for efficacy in malaria therapy. Omidun is the supernatant of fermenting maize (ogi) slurry that has been widely investigated and reported to possess several health benefits and it is used traditionally as solvent for preparing anti-malarial herbs. However, there is no information on the anti-malarial activity of omidun itself. This study was conducted to investigate the prophylactic, curative and suppressive anti-malarial potential of omidun. Methods Experimental mice in the curative group were infected with 1 × 106 cells of Plasmodium berghei strain ANKA and treated with either 0.2 ml of omidun containing 3 × 109 cfu/ml of viable lactic acid bacteria or 0.2 ml of 5 mg/kg of chloroquine (positive control) or 0.2 ml of saline (negative control) for 4 days from day 3 post infection. The prophylactic group of mice were pre-treated with either omidun, chloroquine or saline for 4 days before infection with P. berghei, while the suppressive group was treated with omidun or chloroquine or saline and infected with P. berghei simultaneously. A group of mice were uninfected but treated (with omidun and control samples), while a final group was uninfected and untreated (controls). Parasitaemia and histopathology analysis were done in all groups. Results The curative and suppressive groups showed a significant difference between the omidun-treated mice (100% parasitaemia reduction) and the untreated mice (54.5% parasitaemia increase). There was no significance difference between the omidun treatment and chloroquine (positive control) treatment in suppressive group as both treatment had 100% parasitaemia reduction. The omidun prophylactic treatment however did not show any parasitaemia suppression, but a significant difference was observed between the omidun treatment (85% increase) and the chloroquine (positive control) treatment (100% reduction) in the group. Omidun treatment is non-toxic to the kidney. Conclusion This study provides scientific evidence supporting omidun usage in the treatment of malaria. Consequently, further work may yield the specific component of omidun responsible for the anti-malarial activity.


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