EFFECT OF CURCUMIN FEEDING IN FEED FOR TREATING JAMBAL SIAM FISH (Pangasius hypopthalmus) INFECTED BACTERIA Aeromonas hydrophila

Curcumin is one of the secondary metabolite compounds products that can increase the immune system of fish against bacterial attack. The purpose of this research was to find out the changes in the erythrocytes of a striped fish that were infectious bacteria A. hydrophila and treated with feeding containing curcumin and know the best dose of curcumin extract in the feed. This research was conducted in September to November 2019. The test fish used was the seed of a striped fish that measures 8-10 cm by 150 tails. Method used was the method of experimental and complete random design a factor of 5 levels of treatment and 3 times repeated, the treatment was Kn (feeding without the extract of curcumin and not infected with bacteria A. hydrophila), Kp (feeding without being given curcumin extract and infectious bacteria (A. hydrophila), P1 (addition of curcumin in feed at a dose of 0.7 g/kg), P2 (0.9 g/kg), P3 (of 1.1g/kg). The results shown that P3 treatment was the best treatment with a < 0.05 P where in a total of erythrocytes was 2.46 x 106 cells/mm3. Hemoglobin 9.4 g/dl, hematocrit value of 32.3%, and survival rate was 90%. Curcumin was able to treat fish from an A. hydrophila infection.

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The Effect of Potassium Diformate as Feed Additive on Immune Performances of Nilem (Osteochilus hasselti Valenciennes, 1842) Under Infection of Aeromonas hydrophila

Omni-Akuatika ◽

10.20884/1.oa.2020.16.1.734 ◽

2020 ◽

Vol 16 (1) ◽

pp. 11

Author(s):

Ibnu Bangkit Bioshina Suryadi ◽

Desty Nurul Ulfa ◽

Ayi Yustiati ◽

Rosidah Rosidah

Keyword(s):

Water Quality ◽

Survival Rate ◽

Experimental Method ◽

Aeromonas Hydrophila ◽

Clinical Symptoms ◽

The Body ◽

Effective Dosage ◽

Feed Additive ◽

Random Design ◽

Body Resistance

This research aims to determine the most effective dosage of potassium diformate additives in feed to increase the body resistance of nilem carp (Osteochilus hasselti). This research used Complete Random Design (CRD) experimental method with 4 treatments and 4 replications. The treatment used was the addition of potassium diformate on feed (0 %, 0.1 %, 0.3 % and 0.5 %). After 28 days of treatment, nilem fingerlings were challenged with Aeromonas hydrophila bacteria with a density of 108 cfu ml-1. Parameters observed were the number of leukocytes, erythrocytes, survival rate, clinical symptoms and water quality. The result showed that leukocytes and erythrocytes number were significantly different in each treatment. Meanwhile, leukocytes and erythrocytes number of nilem fingerlings in challenged test were increased and decreased due to the addition of potassium diformate and A. hydrophila infection. Clinical symptoms after challenged test were relatively similar such as haemorhagic, ulceration, exopthalmia, dropsy and abnormal swimming movements. Therefore, survival rate of nilem fingerlings with 0.3 % of potassiun diformate was the highest compared to other treatments, namely 81.67 %.

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Duration of feed application of mixed powders of Phyllanthus niruri and Allium sativum for the prevention of Aeromonas hydrophila infection in catfish Clarias sp.

Jurnal Akuakultur Indonesia ◽

10.19027/jai.11.179-189 ◽

2013 ◽

Vol 11 (2) ◽

pp. 179

Author(s):

Dinamella Wahjuningrum ◽

Iis Widiani ◽

Sri Nuryati

Keyword(s):

Survival Rate ◽

Experimental Design ◽

Aeromonas Hydrophila ◽

Allium Sativum ◽

Phyllanthus Niruri ◽

Random Design ◽

And Control

<p>Motile Aeromonad Septicaemia (MAS) caused by Aeromonas hydrophila induced serious epidemics of disease in catfish Clarias sp. The purposed of this research was to determine optimum durations of feed application of mixed powders Phyllanthus niruri and Allium sativum to prevent MAS. Experimental design that used was Complete Random Design which consist of three treatments and three replications. This research tested on three different of durations of feed application of P. niruri and A. sativum, namely A (powder of P. niruri and A. sativum feed application during 21 days and infected with A. hydrophila 0,1 mL IP), B (powder of P. niruri and A. sativum feed application during 14 days and injected IP with A. hydrophila 0,1 mL), C (powder of P. niruri and A. sativum feed application during seven days and injected IP with A. hydrophila 0,1 mL), and control, namely K- (without P. niruri and A. sativum feed application and injected IP with PBS 0,1 mL) and K+ (without P. niruri and A. sativum powder application and injected IP with A. hydrophila 0,1 mL). The treatments was given for 21 days before challenging test, at 22th day test in vivo carried out by injecting A. hydrophila (108 CFU/mL) into the fish by intramuscular and observed for 10 days. The highest value of survival rate that consist in treatment K- was 100±0.00%, treatment A was 93.3±11.55%, and treatment B was 73.33±30.55%. While treatment K+ and C have the same survival rate that is, 26.67±11.55%. The application P. niruri and A. sativum powder during 21 days was optimum for preventing MAS in catfish.</p><p>Keywords: Phyllanthus niruri, Allium sativum, Aeromonas hydrophila, Clarias sp.</p>

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The effectiveness of honey supplementation in feed for improving goldfish fingerling Carassius auratus immune system against Aeromonas hydrophila bacteria attack

Jurnal Akuakultur Indonesia ◽

10.19027/jai.18.1.89-100 ◽

2019 ◽

Vol 18 (1) ◽

pp. 89-100

Author(s):

Rosidah Rosidah ◽

Ujang Subhan ◽

Yuniar Mulyani ◽

Rifai Dermawan

Keyword(s):

Immune System ◽

Aeromonas Hydrophila ◽

Carassius Auratus ◽

Clinical Symptoms ◽

Design Method ◽

White Blood Cells ◽

Control Treatment ◽

Average Weight ◽

Randomized Design ◽

Bacterial Attack

ABSTRACT The attack of Aeromonas hydrophila bacteria can cause mortality in goldfish approximately 100%. The controlling of this bacterial attack can be done through increased fish immunity. Honey is one of the natural ingredients that increases body immune system. This study aimed to determine the effective dose of honey supplemented in feed to increase goldfish fingerling resistance for disease prevention. Fish used in this study were goldfish fingerlings with 3.5 g average weight. This study was done using experimental complete randomized design method with five treatments and three replications. Treatments given were honey supplementation in feed with 0 ml/kg (A) as control treatment, 150 ml/kg (B), 200 ml/kg (C), 250 ml/kg (D), and 300 ml/kg (E). The result showed that honey supplementation in feed was effective to improve goldfish fingerlings resistance against Aeromonas hydrophila bacterial attack. The supplementation of honey in feed with 200 ml/kg was the best treatment for inducing goldfish fingerlings against A. hydrophila. This was proven by the increased white blood cells (leucocytes) (27.84 ± 5.07%) followed with no apparent clinical symptoms after attacked by A.hydrophila, such as hemorrhage, necrosis, exophthalmia or dropsy, besides showing the highest survival rate with 73.33 ± 11.5%. Keywords : Aeromonas hydophila, goldfish, honey, leucocyte, resistance ABSTRAK Serangan bakteri Aeromonas hydrophila dapat menyebabkan kematian ikan mas koki hingga mencapai 100%. Penanggulangan serangan bakteri tersebut dapat dilakukan dengan meningkatkan ketahanan tubuh (imun) ikan. Madu merupakan salah satu bahan alami yang dapat meningkatkan ketahanan tubuh. Penelitian ini bertujuan untuk menentukan dosis efektif penambahan madu pada pakan untuk meningkatkan daya tahan tubuh benih ikan mas koki dalam upaya pencegahan penyakit aeromonasis. Benih yang digunakan adalah benih ikan mas koki berukuran 3.5 gram. Metode penelitian yang digunakan adalah rancangan acak lengkap (RAL) dengan lima perlakuan dan tiga ulangan. Perlakuan yang digunakan adalah penambahan madu pada pakan dengan dosis 0 mL/kg (A) sebagai kontrol, 150 mL/kg (B), 200 mL/kg (C), 250 mL/kg (D), 300 mL/kg (E). Hasil penelitian menunjukkan bahwa penambahan madu ke dalam pakan efektif dalam meningkatkan ketahanan tubuh ikan mas koki terhadap serangan Aeromonas hydrophila. Dosis 200 ml/kg pakan memberikan hasil terbaik dalam meningkatkan ketahanan tubuh ikan mas koki terhadap serangan A. hydrophila terlihat dari peningkatan jumlah sel darah putih terbesar (27.84 ± 5.07%), tidak nampak adanya gejala klinis ikan terserang A. hydrophila seperti hemoragi, necrosis, exophthalmia maupun dropsy dan menghasilkan kelangsungan hidup benih ikan mas koki tertinggi yaitu sebesar 73.33 ± 11.5%. Kata kunci: Aeromonas hydrophila, ketahanan tubuh, ikan mas koki, madu, sel darah putih.

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NATURAL FEEDING OF SLUDGE WORMS (Tubifex sp.) WITH DIFFERENT FEEDING RATE ON GROWTH AND SURVIVAL RATE OF CTENOPOMA (Ctenopoma acutirostre) FISH SEEDS

Jurnal Mina Sains ◽

10.30997/jms.v5i2.2357 ◽

2019 ◽

Vol 5 (2) ◽

Author(s):

Muhammad Atilah Setiawan ◽

Muarif Muarif ◽

Fia Sri Mumpuni

Keyword(s):

Survival Rate ◽

Feeding Rate ◽

Specific Growth ◽

Feeding Frequency ◽

Initial Weight ◽

Growth And Survival ◽

Randomized Design ◽

Absolute Length ◽

Test Fish ◽

Completely Randomized Design

The aim of this study was to determine the growth and survival rate of Ctenopoma fish seeds. This study used a completely randomized design with 3 treatments and 3 replications. The treatment in this study was a different feeding rate using natural feed of Tubifex sp namely A (8%), B (10%), and C (12%) ). The test fish used was Ctenopoma fish seeds with an initial length of 2.40 ± 0.10 cm and an initial weight of 0.32 ± 0.05 g. Fish seeds are cultivated for 28 days with feeding frequency twice a day. The results of research showed that the use of different feeding rate had an significantly different (P<0.05) on the specific growth rate and absolute length growth with the best results in the treatment C. The survival rate was not significantly different between treatments (P>0.05). Key Words: Ctenopoma, feeding rate, growth, survival rate

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The effect of vaccination in controling bacterial disease caused by Aeromonas hydrophila in nile tilapia (Oreochromis niloticus)

AQUATIC SCIENCE & MANAGEMENT ◽

10.35800/jasm.0.0.2014.7302 ◽

2014 ◽

pp. 27

Author(s):

Arne A Ratulangi ◽

Reiny Tumbol ◽

Hengky Manoppo ◽

Henneke Pangkey

Keyword(s):

Survival Rate ◽

Aeromonas Hydrophila ◽

Oreochromis Niloticus ◽

Nile Tilapia ◽

Bacterial Disease ◽

Immersion Method ◽

Nile Tilapia Oreochromis Niloticus ◽

Different Ages ◽

Motile Aeromonas Septicaemia

This study aims to apply vaccination against bacterial disease. The purpose of vaccination is to trigger the immune respone both non-specific and specific of fish against bacteria Motile Aeromonas Septicaemia (MAS) caused by Aeromonas hydrophila. The vaccination for fish with different ages: 2-3 weeks and 5 weeks were done using immersion method. The fish were re-vaccinated (booster) after two weeks of the first vaccination. The survival rate was < 50 % for juveniles 2-3 weeks and > 50% for juveniles 5 weeks. Survival rate for juveniles 5 weeks was higher than juveniles of 2-3 weeks. This shows that organs of juveniles of 5 weeks were more complete than the 2-3 weeks juveniles. The age of fish is one of the important factors for successfully vaccination. Penelitian ini bertujuan untuk melakukan penerapan vaksinasi terhadap penyakit bakterial. Vaksinasi ditujukan untuk merangsang respon kekebalan non- spesifik dan spesifik pada tubuh ikan terhadap penyakit Motile Aeromonas Septicaemia (MAS) yang disebabkan oleh bakteri Aeromonas hydrophila. Vaksin diberikan pada benih ikan nila yang berbeda umur yaitu 2-3 minggu dan 5 minggu dengan menggunakan metode perendaman. Ikan divaksinasi ulang (booster) setelah 2 (dua) minggu dari vaksinasi yang pertama. Prosentase kelangsungan hidup < 50 % untuk benih umur 2-3 minggu dan > 50% untuk benih umur 5 minggu. Jumlah kematian benih umur 2-3 minggu lebih tinggi dari 5 minggu. Hal ini menunjukkan bahwa fungsi organ benih umur 5 minggu telah lebih lengkap dari pada benih umur 2-3 minggu. Umur ikan merupakan salah satu faktor penting penentu keberhasilan suatu kegiatan vaksinasi.

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The use of β-glucan extracted from baker’s yeast (Saccharomyces cerevisiae) to increase non-specific immune system and resistence of tilapia (Oreochromis niloticus) to Aeromonas hydrophila

AQUATIC SCIENCE & MANAGEMENT ◽

10.35800/jasm.0.0.2013.2288 ◽

2013 ◽

pp. 92

Author(s):

Ida N Jamal ◽

Reiny A Tumbol ◽

Remy E.P Mangindaan

Keyword(s):

Saccharomyces Cerevisiae ◽

Immune System ◽

Aeromonas Hydrophila ◽

Phagocytic Activity ◽

Baker’S Yeast ◽

Baker's Yeast ◽

Yeast Saccharomyces Cerevisiae ◽

Randomized Design ◽

The Difference ◽

Motile Aeromonas Septicaemia

Motile Aeromonas Septicaemia disease (MAS) attacking tilapia has increased in recent years as a consequence of intensive aquaculture activities, which led to losses in aquaculture industry. The agent causing MAS disease is Aeromonas hydrophila. The disease can be controlled with the β-glucan. As immunostimulants, β-glucans can also increase resistance in farmed tilapia. Studies on the use of β-glucan extracted from baker's yeast Saccharomyces cerevisiae was intended to evaluate the non-specific immune system of tilapia that were challenged with Aeromonas hydrophila. The method used was an experimental method with a completely randomized design consisting of four treatments with three replicats. The dose of β-glucan used as treatments were 0 mg.kg-1 fish (Control), 5 mg.kg-1 fish (B), 10 mg.kg-1 fish (C) and 20 mg.kg-1 fish (D), each treatment as injected three times at intervals of 3 days, the injection volume of 0.5 ml/fish for nine days and resistance surveillance for seven days. The results showed that the difference in the amount of β-glucan and the frequency of the injected real influence on total leukocytes, phagocytic activity and resistance. Total leukocytes, phagocytic activity and resistance to treatment was best achieved by the administration of C a dose of 10 mg.kg-1 of the fish© Penyakit Motil Aeromonas Septicaemia (MAS) yang menyerang ikan nila mengalami peningkatan selama beberapa tahun terakhir sebagai konsekuensi dari kegiatan akuakultur intensif, yang menyebabkan kerugian dalam industri budidaya. Agen utama penyebab penyakit MAS adalah Aeromonas hydrophila. Untuk mengendalikan penyakit tersebut dapat dilakukan dengan pemberian β-glukan. Sebagai imunostimulan, β-glukan juga dapat meningkatkan resistensi pada ikan nila yang dibudidayakan. Pengkajian mengenai pemanfaatan β-glukan yang diekstrak dari ragi roti Saccharomyces cerevisiae dimaksudkan untuk menguji sistem imun non spesifik ikan nila yang diuji tantang dengan bakteri Aeromonas hydrophila. Metode yang digunakan yaitu metode eksperimen dengan rancangan acak lengkap yang terdiri dari empat perlakuan dan tiga ulangan. Dosis β-glukan yang digunakan sebagai perlakuan sebesar 0 mg.kg-1 ikan (Kontrol), 5 mg.kg-1 ikan (B), 10 mg.kg-1 ikan (C) dan 20 mg.kg-1 ikan (D), masing-masing perlakuan diinjeksi sebanyak 3 kali dengan interval waktu 3 hari selama 9 hari, volume injeksi 0,5 mL/ekor ikan dan pengamatan resistensi selama tujuh hari. Hasil penelitian menunjukkan perbedaan jumlah β-glukan dan frekuensi pemberian yang diinjeksikan memberikan pengaruh nyata terhadap total leukosit, aktivitas fagositosis dan resistensi. Total leukosit, aktivitas fagositosis dan resistensi terbaik dicapai pada perlakuan C dengan dosis 10 mg.kg-1 ikan©

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