scholarly journals The effectiveness of honey supplementation in feed for improving goldfish fingerling Carassius auratus immune system against Aeromonas hydrophila bacteria attack

2019 ◽  
Vol 18 (1) ◽  
pp. 89-100
Author(s):  
Rosidah Rosidah ◽  
Ujang Subhan ◽  
Yuniar Mulyani ◽  
Rifai Dermawan

ABSTRACT The attack of Aeromonas hydrophila bacteria can cause mortality in goldfish approximately 100%. The controlling of this bacterial attack can be done through increased fish immunity. Honey is one of the natural ingredients that increases body immune system. This study aimed to determine the effective dose of honey supplemented in feed to increase goldfish fingerling resistance for disease prevention. Fish used in this study were goldfish fingerlings with 3.5 g average weight. This study was done using experimental complete randomized design method with five treatments and three replications. Treatments given were honey supplementation in feed with 0 ml/kg (A) as control treatment, 150 ml/kg (B), 200 ml/kg (C), 250 ml/kg (D), and 300 ml/kg (E). The result showed that honey supplementation in feed was effective to improve goldfish fingerlings resistance against Aeromonas hydrophila bacterial attack. The supplementation of honey in feed with 200 ml/kg was the best treatment for inducing goldfish fingerlings against A. hydrophila. This was proven by the increased white blood cells (leucocytes) (27.84 ± 5.07%) followed with no apparent clinical symptoms after attacked by A.hydrophila, such as hemorrhage, necrosis, exophthalmia or dropsy, besides showing the highest survival rate with 73.33 ± 11.5%. Keywords : Aeromonas hydophila, goldfish, honey, leucocyte, resistance ABSTRAK Serangan bakteri Aeromonas hydrophila dapat menyebabkan kematian ikan mas koki hingga mencapai 100%. Penanggulangan serangan bakteri tersebut dapat dilakukan dengan meningkatkan ketahanan tubuh (imun) ikan.  Madu merupakan salah satu bahan alami yang dapat meningkatkan ketahanan tubuh. Penelitian ini bertujuan untuk menentukan dosis efektif penambahan madu pada pakan untuk meningkatkan daya tahan tubuh benih ikan mas koki dalam upaya pencegahan penyakit aeromonasis. Benih yang digunakan adalah benih ikan mas koki berukuran 3.5 gram. Metode penelitian yang digunakan adalah rancangan acak lengkap (RAL) dengan lima perlakuan dan tiga ulangan. Perlakuan yang digunakan adalah penambahan madu pada pakan dengan dosis 0 mL/kg (A) sebagai kontrol, 150 mL/kg (B), 200 mL/kg (C), 250 mL/kg (D), 300 mL/kg (E). Hasil penelitian menunjukkan bahwa penambahan madu ke dalam pakan efektif dalam meningkatkan ketahanan tubuh ikan mas koki terhadap serangan Aeromonas hydrophila. Dosis 200 ml/kg pakan memberikan hasil terbaik dalam meningkatkan ketahanan tubuh ikan mas koki terhadap serangan A. hydrophila terlihat dari peningkatan jumlah sel darah putih terbesar (27.84 ± 5.07%), tidak nampak adanya gejala klinis ikan terserang A. hydrophila seperti hemoragi, necrosis, exophthalmia maupun dropsy dan menghasilkan kelangsungan hidup benih ikan mas koki tertinggi yaitu sebesar 73.33 ± 11.5%. Kata kunci: Aeromonas hydrophila, ketahanan tubuh, ikan mas koki, madu, sel darah putih. 

Author(s):  
Ida N Jamal ◽  
Reiny A Tumbol ◽  
Remy E.P Mangindaan

Motile Aeromonas Septicaemia disease (MAS) attacking tilapia has increased in recent years as a consequence of intensive aquaculture activities, which led to losses in aquaculture industry. The agent causing MAS disease is Aeromonas hydrophila. The disease can be controlled with the β-glucan. As immunostimulants, β-glucans can also increase resistance in farmed tilapia. Studies on the use of β-glucan extracted from baker's yeast Saccharomyces cerevisiae was intended to evaluate the non-specific immune system of tilapia that were challenged with Aeromonas hydrophila. The method used was an experimental method with a completely randomized design consisting of four treatments with three replicats. The dose of β-glucan used as treatments were 0 mg.kg-1 fish (Control), 5 mg.kg-1 fish (B), 10 mg.kg-1 fish (C) and 20 mg.kg-1 fish (D), each treatment as injected three times at intervals of 3 days, the injection volume of 0.5 ml/fish for nine days and resistance surveillance for seven days. The results showed that the difference in the amount of β-glucan and the frequency of the injected real influence on total leukocytes, phagocytic activity and resistance. Total leukocytes, phagocytic activity and resistance to treatment was best achieved by the administration of C a dose of  10 mg.kg-1 of the fish© Penyakit Motil Aeromonas Septicaemia (MAS) yang menyerang ikan nila mengalami peningkatan selama beberapa tahun terakhir sebagai konsekuensi dari kegiatan akuakultur intensif, yang menyebabkan kerugian dalam industri budidaya. Agen utama penyebab penyakit MAS adalah Aeromonas hydrophila. Untuk mengendalikan penyakit tersebut dapat dilakukan dengan pemberian β-glukan. Sebagai imunostimulan, β-glukan juga dapat  meningkatkan resistensi pada ikan nila yang dibudidayakan. Pengkajian mengenai pemanfaatan β-glukan yang diekstrak dari ragi roti Saccharomyces cerevisiae dimaksudkan untuk menguji sistem imun non spesifik ikan nila yang diuji tantang dengan bakteri Aeromonas hydrophila. Metode yang digunakan yaitu metode eksperimen dengan rancangan acak lengkap yang terdiri dari empat perlakuan dan tiga ulangan. Dosis β-glukan  yang digunakan sebagai perlakuan sebesar 0 mg.kg-1 ikan (Kontrol), 5 mg.kg-1 ikan (B), 10 mg.kg-1 ikan (C) dan 20 mg.kg-1 ikan (D), masing-masing perlakuan diinjeksi sebanyak 3 kali dengan interval waktu 3 hari selama 9 hari, volume injeksi 0,5 mL/ekor ikan dan pengamatan resistensi selama tujuh hari. Hasil penelitian menunjukkan perbedaan jumlah β-glukan dan frekuensi pemberian yang diinjeksikan memberikan pengaruh nyata terhadap total leukosit, aktivitas fagositosis dan resistensi. Total leukosit, aktivitas fagositosis dan resistensi terbaik dicapai pada perlakuan C dengan dosis 10 mg.kg-1 ikan©


2022 ◽  
Vol 6 (6) ◽  
Author(s):  
Feren Feren ◽  
Sri Wahyuni ◽  
RH Fitri Faradilla

ABSTRACT The purpose of this study was to determine the interaction effect of storage time and concentration of plantain peel extract and lemongrass on organoleptic characteristics (color, aroma, texture, and elasticity) and total microbes in chicken meatballs. This study used a 2-Factorial Completely Randomized Design method. The first factor was storage time with three levels, namely 0 days (T0), 2 days (T1), and 4 days (T2. Meanwhile, the second factor was the concentration of plantain peel and lemongrass extract with three levels, namely 0% (C0), 30% plantain peel extract + 20% lemongrass extract (C1), 20% banana peel extract + 30% lemongrass extract (C2), and 25% banana peel extract + 25% lemongrass extract (C3). Observation variables were organoleptic tests which included color, aroma, texture, elasticity, and shelf life after treatment based on the results of the total plate count (TPC) calculation. The results show that the 2-day storage period with the addition of 25% plantain peel extract and 25% lemongrass extract (T1C3) was the best treatment for organoleptic characteristics (color, aroma, texture, and elasticity). Based on the results of the study, it can be concluded that the administration of plantain peel extract and lemongrass was able to maintain the quality of chicken meatballs for two different days compared to the control. Treatment with a storage period of 2 days with 25% plantain peel extract and 25% lemongrass extract (T1C3) was the best treatment that was most favored by the panelists and had the least number of microbial colonies.Keywords: Meatballs, preservatives, banana peel extract and lemongrass, storage time.ABSTRAKTujuan penelitian ini adalah untuk mengetahui pengaruh interaksi lama penyimpanan dan konsentrasi ekstrak kulit pisang raja dan serai terhadap uji organoleptik (warna, aroma, tekstur dan kekenyalan) dan total mikroba pada bakso ayam. Penelitian ini menggunakan metode Rancangan Acak Lengkap Faktorial 2 Faktor. Faktor Pertama adalah lama penyimpanan yang terdiri dari tiga taraf yaitu 0 hari (T0), 2 hari (T1) dan 4 hari (T2), faktor kedua yaitu perlakuan ekstrak kulit pisang raja yang terdiri atas tiga taraf yaitu konsentrasi 0% (C0), konsentrasi ekstrak kulit pisang raja 30% + konsentrasi ekstrak serai 20% (C1), konsentrasi ekstrak kulit pisang raja 20% + konsentrasi ekstrak serai 30% (C2), konsentrasi ekstrak kulit pisang raja 25% + konsentrasi serai 25% (C3). Variabel pengamatan yaitu uji organoleptik yang meliputi warna, aroma, tekstur, kekenyalan dan daya simpan setelah perlakuan berdasarkan hasil perhitungan jumlah total plate count (TPC). Hasil penelitian menunjukkan bahwa perlakuan lama penyimpanan 2 hari dengan penambahan ekstrak kulit pisang raja 25% dab serai 25% (T1C3) merupakan perlakuan terbaik untuk uji organoleptik (warna, aroma, tekstur dan kekenyalan). Berdasarkan hasil penelitian dapat disimpulkan bahwa pemberian ekstrak kulit pisang raja dan serai mampu mempertahankan kualitas bakso ayam selama dua hari yang berbeda dengan kontrol. Perlakuan dengan lama penyimpanan 2 hari dengan pemberianekstrak kulit pisang raja 25% dan serai 25% (T1C3) merupakan perlakuan terbaik yang paling disukai oleh panelis dan memiliki jumlah koloni mikroba paling sedikit.Kata kunci: Bakso, pengawet, ekstrak kulit pisang dan serai, lama penyimpanan


2021 ◽  
Vol 21 (3) ◽  
pp. 277-290
Author(s):  
Yuli Andriani ◽  
Zahidah Zahidah ◽  
Rosidah Rosidah ◽  
Iskandar Iskandar

This research aims to determine the effect of fine bubble technology with different pressures on Siamese catfish seed's growth and feed efficiency in an aquaponic system. This research used a completely randomized design with four treatments and three replications. Treatment A (Control), Treatment B (FBs with a pressure of 4.5 atm), Treatment C (FBs with a pressure of 5 atm and treatment D (FBs with a pressure of 5.5 atm). The parameters observed were Specific Growth Rate, Feed Conversion Ratio, water quality (temperature, pH, DO, and ammonia), and plant productivity.  The seeds used were catfish seeds aged 16-36 days, 1-2 inches long, and weighed 0.26-0.29 grams. The container used is a fiber tub of 16 units with a size of 70 cm x 70cm x 70 cm. The feed used is commercial feed PF 1000 content crude protein 35%. The data were analyzed using variance with the F test at the 95% confidence level. The best treatment for catfish growth was treatment D (pressure 5.5 atm), which gave the highest SGR value of 7.24 ± 0.5 and FCR value of 1.19 ± 0.09. The value of water quality parameters is in suitable condition for the growth of catfish seed. The best plant productivity was found in treatment D with an average weight of 60.75 g.


2017 ◽  
Vol 4 (1) ◽  
pp. 37
Author(s):  
Muhammad Fazil ◽  
Saiful Adhar ◽  
Riri Ezraneti

Penelitian ini dilakukan di Laboratorium Hatchery dan Teknologi Budidaya Program Studi Budidaya Perairan Fakultas Pertanian Universitas Malikussaleh, dimulai pada bulan Desember 2015 sampai dengan Januari 2016. Ikan uji yang digunakan adalah ikan mas koki yang berukuran 3-4 cm. Tujuan penelitian ini adalah untuk mengetahui pengaruh bahan filter dari ijuk, jerami padi dan ampas tebu sebagai filter air pada pemeliharaan ikan mas koki. Penelitian ini menggunakan metode eksperimental dengan Rancangan Acak Lengkap (RAL) non faktorial dengan empat perlakuan dan tiga kali ulangan. Pertambahan panjang terbesar terdapat pada bahan filter ijuk yaitu 1,14 cm. Pertambahan berat terbesar terdapat pada bahan filter ijuk yaitu 1,29 gram dan terkecil pada perlakuan kontrol yaitu 0,42 gram. Nilai kisaran parameter kualitas air pada saat penelitian yaitu suhu berkisar 25,7-29,7 oC, pH berkisar 7,1-7,6, DO berkisar 3,6-5,8 mg/L, kekeruhan berkisar 1,14-22,15 dan amonia berkisar 0,022-2,056.This research was conducted at the Laboratory of Aquaculture Hatchery and Technology Studies Program Aquaculture Faculty of Agriculture, University of Malikussaleh, started in December 2015 and January 2016. The fish samples used is a goldfish measuring 3-4 cm. The purpose of this study was to determine the effect of filter material from fibers, rice straw and bagasse as a water filter on the maintenance of a goldfish. This research used experimental method with a completely randomized design (CRD) non factorial with four treatments and three replications. Added greatest long fibers present in the filter material is 1.14 cm. The weight gain fibers contained in the filter material is 1.29 grams and the smallest in the control treatment that is 0.42 grams. Value range of water quality parameters at the time of the study ranged from 25.7 to 29.7 ° C as temperature, pH ranges from 7.1 to 7.6, DO ranged from 3.6 to 5.8 mg / L, turbidity ranges from 1.14 to 22 , 15 and ammonia ranged from 0.022 to 2.056.


2020 ◽  
Vol 4 (2) ◽  
pp. 48-53
Author(s):  
Muhammad Nur Hidayat ◽  
Amina Hajah Thaha ◽  
Reny Mayanti

This study aims to determine the effect of noni leaf (Morinda citrifolia lignosae) extract as a natural disinfectant on the percentage of hatchability and the hatchability weight of quail (Coturnix-coturnix japonica). This study used 1,500 quail hatch eggs with a male and female parent ratio of 1: 4. This study used a Completely Randomized Design (CRD) method, with five treatments and three replications each repetition consisting of 100 quail eggs with 10-gram weight. The treatments used were: T0 (control treatment), T1 (commercial disinfectant), T2 (noni leaf extract 10%), T3 (noni leaf extract 20%) and T4 (noni leaf extract 30%). The results of variance showed the average percentage of hatchability produced during the study, namely T0 (85%), T1 (83%), T2 (86%), T3 (84%), and T4 (93%). Furthermore, the average weight of hatchability produced, namely T0 (6.67 grams), T1 (6.76 grams, T2 (6.89 grams), T3 (6.84 grams) and T4 (6.89 grams). Overall administration of noni leaf extract had no significant effect (P> 0.05) on the percentage of hatchability and DOQ hatching weight of the quail.


2019 ◽  
Vol 1 (1) ◽  
pp. 13-18
Author(s):  
Satria Wati Pade

Pineapple is desirable to be consumed fresh, but it taking too long in stripping the pineapple's outer skin. Therefore, currently, the technology of processing horticultural products is minimally applied. Minimum fruit processing can speed up product shelf life. One way to overcome this is coating. Hydrocolloid edible coating is one type of coating with starch as the base material found in several types of tubers, one of which is cassava tuber. This study used a completely randomized design method consisting of one control sample and 3 treatments, cassava starch concentrations were repeated three times, namely A1: 3% cassava starch, A2: 4% cassava starch, A3: 5% cassava starch with 8 days observation. The variables observed were vitamin C, weight loss and color. The results showed that the highest vitamin C content was in the treatment of 5% cassava starch at 3.20 mg/100 g and the lowest was the control treatment of 3.15 mg/100 g. The smallest weight loss was A3 treatment of 73.75% and the biggest was control treatment 79.67%. The average value of the highest color was found in A3 treatment, which was around 66.92º showed yellow-red.


2019 ◽  
Vol 40 (6Supl3) ◽  
pp. 3595
Author(s):  
Andrezza Kyarelle Bezerra de Moura ◽  
Renata Nayhara de Lima ◽  
Kátia Tatiana de Lima Lopes ◽  
João Artur de Lima Neto ◽  
Vítor Lucas de Lima Melo ◽  
...  

The objective of this study was to evaluate the performance of calves (Holstein × No Defined Breed Standard) fed powdered cheese whey associated with discarded powdered milk up to 60 days of age. Forty calves (35 kg initial average weight) were used, distributed in a completely randomized design with four treatments and ten repetitions per treatment: whole milk (control), 100% milk powder, 80% milk powder + 20% cheese whey powder, 60% milk powder + 40% cheese whey powder. Consumption, performance, and economic analysis were evaluated. Data were analyzed for variance and comparison of orthogonal contrasts (P < 0.05). No significant differences were observed between the treatments tested regarding the evaluated variables. The animals from all treatments had a final average weight of 50.28 kg as the result of an average weight gain of 0.255 ± 0.03 kg/day and a total dry matter intake of 34.28 ± 1.47 kg/day per calf. The examined diets offered the lowest cost compared to the control treatment, generating an average additional profit of 113.19 R$/animal. Diets containing whey cheese powder are the most economically advantageous options, as replacing 100% of milk with these by-products did not impair animal performance. The use of discarded industrial milk powder combined with whey powder in a ratio of up to 80:20 is a viable option for feeding male calves in dairy farms.


2019 ◽  
Vol 9 (2) ◽  
Author(s):  
Mulyana Mulyana ◽  
Anjas Friyana Sukandar ◽  
Fia Sri Mampuni

The experimental research was carried out on March to August, 2018, at The Fisheries Laboratory, Djuanda University, Bogor. The research is aimed to know and analyze the blood picture of hard-lipped barb that infected by Aeromonas hydrophila.  The experimental research used complete randomized design with 7 treatments and each 2 replications. The treatments are Control (without A. hydrophila injection), A (with 104 cfu/mL injection), B (with 105 cfu/mL injection),  C (with 106 cfu/mL injection), D (with 107 cfu/mL injection), E (with 108 cfu/mL injection), and F (with 109 cfu/mL injection). The other than Control treatment, each fish in the experimental research was injected via intramuscular with a virulen strain Aeromonas hydrophila bacteria according to the dose of treatment as much as 0.1 mL. The results of research showed that total erythrocytes 1,01 x 106 sel/mm3-1,31 x 106 sel/mm3 and the hemoglobin 2,3 g%-5,2 g% levels have experienced decrease, while total leucocytes 4,32 x 104 sel/mm3-7,53 x 104 sel/mm3 have experienced increase and  hematocrite 19,2%-21,4% levels were stable.


AGRIFOR ◽  
2018 ◽  
Vol 17 (2) ◽  
pp. 345
Author(s):  
Sopialena Sopialena

Research on the effect of Trichoderma sp. In tomato plants on the factors of production is a study carried out for 4 months starting from June to October 2017. The research was carried out at the Green House of the Faculty of Agriculture, Mulawarman University, Samarinda.This research was conducted using a completely randomized design (CRD) with each treatment repeated 3 times. The treatment is Po: Without Trichoderma sp. / Control treatment; P1: 25 g culture of Trichoderma sp. per polybag; P2: 30 g of mushroom culture Trichoderma sp. per polybag; P3: 35 g of mushroom culture Trichoderma sp. Per polybag; and P4: 40 g culture of Trichoderma sp. per polybag As the second factor, tomato varieties include V1: Lentana; V2: Permata and V3: Ratna. The data observed included the number of planting fruit (calculated from the first harvest to the last harvest for each crop); Average diameter of planting fruit and. The average weight of fresh fruit plantations.The results showed that the dose of 40g Trichoderma sp. most effective in controlling F. oxysporum wilt disease on tomato plants, which can increase tomato crop production by 293.48 g. Variety treatment was not significantly different from all treatments. So that there is no interaction between Trichoderma sp. and tomato varieties.


2021 ◽  
Vol 1 (1) ◽  
pp. 29-39
Author(s):  
Ani Lestari ◽  
Nurliah ◽  
Baiq Hilda Astriana

Research on the effect of garlic extract (allium sativum) to treat tilapia (oreochromis niloticus) seeds which are infected with aeromonas hydrophila bacteria. This research was conducted for 14 days from 18 December to 31 December 2019 which took place at the Wet Laboratory of the Aquaculture Study Program, Mataram University, West Nusa Tenggara (NTB). This study used a completely randomized design (CRD) with an experimental method consisting of 5 treatments and 3 replications. The container used in this study was a 15 liter jar with a volume of 10 liters. The containers used are 15 units, where in treatment one (P1) using without soaking garlic extract and without being infected with Aeromonas hydrophila bacteria, treatment two (P2) without soaking garlic extract and being infected with Aeromonas hydrophila bacteria, (P3) using soaking onion extract white with a concentration of 1% and infected with Aeromonas hydrophila bacteria, (P4) using garlic extract immersion with a concentration of 1.5% and infected with Aeromonas hydrophila bacteria, (P5) using garlic extract immersion with a concentration of 2% and infected with Aeromonas hydrophila bacteria. The parameters observed during the study included survival rate (SR), relative percent survival (RPS), observation of clinical symptoms, and water quality. The results showed that giving garlic extract with different concentrations had a significantly different effect on survival rates and relative percent survival (RPS).


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