scholarly journals Cloning of Phenylalanine Ammonia-lyase and its Role in Enzymatic Browning of Mung Bean Sprout during Cold Storage

2016 ◽  
Vol 22 (2) ◽  
pp. 255-260 ◽  
Author(s):  
Nana Sameshima ◽  
Midori Nishimura ◽  
Koko Murakami ◽  
Yukako Kogo ◽  
Yuko Shimamura ◽  
...  
2018 ◽  
Vol 24 (4) ◽  
pp. 573-581 ◽  
Author(s):  
Yukako Kogo ◽  
Nana Sameshima ◽  
Yuki Ukena ◽  
Satomi Tsutsuura ◽  
Masatsune Murata

2012 ◽  
Vol 18 (3) ◽  
pp. 413-417 ◽  
Author(s):  
Midori NISHIMURA ◽  
Nana SAMESHIMA ◽  
Keiko JOSHITA ◽  
Masatsune MURATA

Foods ◽  
2022 ◽  
Vol 11 (2) ◽  
pp. 160
Author(s):  
Francisca Hernández ◽  
Lucía Andreu-Coll ◽  
Andreia Bento-Silva ◽  
Ana Teresa Serra ◽  
Pedro Mena ◽  
...  

This research analyzed the phytochemical profile of prickly pear fruits from ‘Orito’ cultivar stored under cold conditions (2 °C, 85–90% RH) and shelf-life conditions at room temperature (stored at 20 °C for three days after cold storage) for 28 days, mimicking the product life cycle. A total of 18 compounds were identified and quantitated through HPLC-DAD-MS/MS (High-Performance Liquid Chromatographic -Diode Array Detector- Mass Spectrometry) analyses. Phenolic acids such as eucomic acid and betalains such as indicaxanthin were the predominant chemical families, and piscidic acid was the most abundant compound. During cold storage, the content of eucomic acid isomer/derivative and syringaresinol increased, and citric acid decreased, which could be caused by the cold activation of the phenylalanine ammonia-lyase (PAL) and polyphenol oxidase (PPO) enzymes. However, no significant differences were found in the content of these compounds during shelf-life storage. These results showed that ‘Orito’ fruit marketability would be possible up to 28 days after harvesting, retaining its profile, which is rich in bioactive compounds.


2007 ◽  
Vol 54 (6) ◽  
pp. 247-252 ◽  
Author(s):  
Isao Hayami ◽  
Yoshie Motomura ◽  
Takashi Nishizawa

2019 ◽  
Vol 27 (1) ◽  
pp. 24 ◽  
Author(s):  
Widati Fatmaningrum ◽  
Woro Setia Ningtyas

Objectives: The purpose of this study was to analyze the effects of mung bean sprout extracts on Follicle stimulating hormone (FSH) and estrogen hormone in female Wistar rats (Rattus norvegicus) exposed to monosodium glutamate (MSG).Materials and Methods: This true experimental study was conducted by using post-test only control group design in the laboratory for animal experimentation of Faculty of Veterinary Medicine - Airlangga University in which Wistar rats (Rattus norvegicus) aged 2 months weighing 150-200 grams were used in this experiment. The samples comprised of 5 rats distributed in each group, totaling 7 groups. I Control Group (P1) was provided with Aquades for 37 days; II (P2) was provided with Aquades for 7 days + MSG 0.03 mg/g of weight on day 8-37; III (P3) was provided with extract of mung bean sprouts 72mg/200g of weight on day 1-37 + MSG 0,03mg/g of weight; IV (P4) was provided with extract of mung bean sprouts 144mg/200g of weight on day 1-37 + MSG 0.03mg/g of weight; V (P5) was provided with Aquades for 7 days + MSG 0.7 mg/g of weight on day 8-37; VI (P6) was provided with extract of mung bean sprouts 72mg/200g of weight on day 1-37 + MSG 0.7mg/g of weight; VII (P7) was provided with extract of mung bean sprouts 144mg/200g of weight on day 1-37 + MSG 0.7mg/g of weight.Serum samples were taken for Follicle Stimulating Hormone (FSH) and Estrogen with ELISA method. Data analysis to test the differences between groups was done by using one way Anova statistical test.Results: MSG dosage 0,03mg/gBB or 0,7mg/g BB was significant difference in FSH (p = 0,011) and estrogen (p = 0,008).Conclusion: obtained from this research that giving green bean sprout extract influence to the level of FSH and estrogen hormone.


Horticulturae ◽  
2021 ◽  
Vol 7 (10) ◽  
pp. 373
Author(s):  
Geerada Kaewjumpol ◽  
Surasak Srisamlee ◽  
Diane M. Beckles ◽  
Kietsuda Luengwilai

Banana blossoms are rich in fiber and nutrients and are a popular plant-based, vegan alternative to fish. However undesirable browning, usually visible at the peduncle cut-end, negatively impacts consumer acceptability of banana blossoms. The aim of this work was to develop safe alternatives to prevent browning in banana blossoms. First, the activities of primary enzymes associated with tissue browning, i.e., polyphenol oxidase (PPO), peroxidase (POD) and phenylalanine ammonia lyase (PAL), were assayed. Our data showed that PPO and POD were the key enzymes responsible for blossom browning as they increased in activity, reaching a maximum at pH 7, as browning developed. In contrast, PAL activity decreased, and total phenolic content did not change as browning progressed, indicating PAL was not induced by cutting and may not be involved in blossom browning. Second, to find antibrowning agents for banana blossoms that can substitute for the use of sodium metabisulfite (SMS), different organic acids of varying concentrations were tested. Among organic acids studied, treatment with 3% (w/v) oxalic acid was the most effective method and thus could be a safe substitution for SMS to prevent browning in banana blossoms.


Author(s):  
Azham Purwandhono ◽  
Rena Normasari

Coronary heart disease is an important health problem. It is estimated that the death rate from coronary heart disease by 23,4 millions in 20130 (WHO, 2009). Excessive physical activity can cause oxidative stress which lead to atherosclerosis. Mung bean sprouts (Vigna radiata (L)) contain vitamin E, vitamin C, fenol, flavonoid, fitosterol, and other minerals. The aim of this study is to determine the effect of mung beans sprout extract on on the thicknes of the tunica intima-media on physical stress-induced atherosclerosis of male wistar rat. This research is true experimental study using 20 male wistar rat were divided into five groups: negative control, positive control and treatment groups P1, P2, P3 (physical stress + bean sprout extract 50 mg/day, 100 mg/day, 200 mg/day for 10 days). There is significant decrease of MDA serum level in the treatment groups.Keywords : Vigna radiata, physical stress, thicknes of tunica intima-media


2019 ◽  
Vol 1241 ◽  
pp. 012015
Author(s):  
M Ilmi ◽  
L K Putri ◽  
A A K Muhamad ◽  
A Cholishoh ◽  
S A Ardiansyah

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