scholarly journals Effect of Mung Bean Sprout (Vigna radiata (L)) Extract on Physical Stress-Induced Atherosclerosis of Male Wistar Rat

1970 ◽  
pp. 26
Author(s):  
Azham Purwandhono ◽  
Rena Normasari
Keyword(s):  
Coronary Heart Disease ◽  
Heart Disease ◽  
Vigna Radiata ◽  
Mung Bean ◽  
Wistar Rat ◽  
Positive Control ◽  
Physical Stress ◽  
Negative Control ◽  
Bean Sprout ◽  
Tunica Intima

Coronary heart disease is an important health problem. It is estimated that the death rate from coronary heart disease by 23,4 millions in 20130 (WHO, 2009). Excessive physical activity can cause oxidative stress which lead to atherosclerosis. Mung bean sprouts (Vigna radiata (L)) contain vitamin E, vitamin C, fenol, flavonoid, fitosterol, and other minerals. The aim of this study is to determine the effect of mung beans sprout extract on on the thicknes of the tunica intima-media on physical stress-induced atherosclerosis of male wistar rat. This research is true experimental study using 20 male wistar rat were divided into five groups: negative control, positive control and treatment groups P1, P2, P3 (physical stress + bean sprout extract 50 mg/day, 100 mg/day, 200 mg/day for 10 days). There is significant decrease of MDA serum level in the treatment groups.Keywords : Vigna radiata, physical stress, thicknes of tunica intima-media

scholarly journals The Comparison of Bay Leaf and Celery Leaf Infusion Effect on Decreasing LDL Level in Dyslipidemic Wistar Rats Model

1970 ◽  
Vol 1 (1) ◽  
pp. 21
Author(s):  
Anjani Putri Retnaninggalih ◽  
Erfan Efendi ◽  
Hairrudin Hairrudin
Keyword(s):  
Cholesterol Level ◽  
High Fat Diet ◽  
Wistar Rats ◽  
Ldl Cholesterol ◽  
Wistar Rat ◽  
Positive Control ◽  
Negative Control ◽  
High Fat ◽  
Leaf P ◽  
One Way Anova

Coronary heart disease (CHD) is the cause of 7,3 million death globally in 2008. The main pathophysiology process that leads to CHD is atherosclerosis which is caused by high LDL cholesterol level. The aim of this study was to explore bay leaf and celery leaf infusion effect on reducing LDL cholesterol level in dyslipidemic rat model. The type of this study was true experimental research using wistar rat (Rattus novergicus) as the sample. The rats were induced by high fat diet and PTU for two weeks then each of the group were given either bay leaf infusion, celery leaf infusion, simvastatin (positive control), or aquadest (negative control) for a week. The measurement of LDL level used Friedewald formulation. Data was analyzed using paired sample t test and one way ANOVA. As the result there was a significance reduction on LDL level after treatment with bay leaf (p=0,013) and celery leaf infusion (p=0,035) but there was no significance difference between groups (p=0,293). It was concluded that bay leaf and celery leaf infusion could reduce the LDL level in dyslipidemic wistar rat but there was no difference on the capability of the two different leafs on reducing LDL level. Keywords: dyslipidemia, LDL, bay leaf, celery leaf, wistar rats

scholarly journals Cardioprotective Potential of Ethanol Extract of Sonchus Arvensis L. Leaves on Isoproterenol-Induced Myocardial Infarction in Rat

2018 ◽  
Vol 16 (1) ◽  
pp. 20
Author(s):  
Neng Fisheri Kurniati ◽  
Elin Yulinah Sukandar ◽  
Rian Pardilah ◽  
Nova Suliska ◽  
Dhyan Kusuma Ayuningtyas
Keyword(s):  
Myocardial Infarction ◽  
Creatine Kinase ◽  
Ethanol Extract ◽  
Wistar Rat ◽  
Positive Control ◽  
Negative Control ◽  
Albino Rats ◽  
Creatine Kinase Mb ◽  
Wistar Albino Rats ◽  
Sonchus Arvensis

Sonchus arvensis L. leaves have been used traditionally to treat various disease conditions. This study is designed to evaluate cardioprotective potential of ethanol extract of S. arvensis leaves on isoproterenol-induced myocardial infarction in Wistar rat. Male Wistar albino rats were divided into three main groups: negative control (saline only), positive control (isoproterenol only), and S. arvensis extract treated groups. S. arvensis extract was administered in three doses; 50, 100, and 200 mg/kg b.w. p.o for 14 days. On day 13 and 14, isoproterenol (85 mg/kg bw) was given intraperitoneally to positive control and extract treated groups. The parameters studied were cardiac biomarker enzymes which were Creatine Kinase (CK), Creatine Kinase-MB (CK-MB), Aspartate Transaminase (AST), Alanine Transaminase (ALT) and Lactate Dehydrogenase (LDH). The results showed that S. arvensis at dose of 50 mg/kg b.w. could significantly (P<0.05) reduce the level of CK, CK-MB, AST, ALT, and LDH in myocardial infarcted rats compared to positive control. The increase of the dose of S. arvensis extract was not followed by an increase of its cardioprotective activity. In conclusion, Sonchus arvensis L. leaves extract at dose of 50 mg/kg b.w. has potential to be developed as cardioprotective drug.

scholarly journals Effect of Sodium Carboxymethylcellulose and Sorbitol on Anti-Peptic Ulcer Activity of Anredera cordifolia Leaves Extract

2019 ◽  
Vol 4 (1) ◽  
pp. 16
Author(s):  
Maria Ulfah ◽  
Revika Rachmaniar ◽  
Egi MR. Sudrajat ◽  
Rida W. Fadla ◽  
Hary S. Pinuji
Keyword(s):  
Body Weight ◽  
Peptic Ulcer ◽  
Normal Control ◽  
Wistar Rat ◽  
Positive Control ◽  
Negative Control ◽  
Sodium Carboxymethylcellulose ◽  
Freeze Dried ◽  
Male Wistar Rats ◽  
Negative Controls

Anredera cordifolia or binahong is one of the Indonesian medicinal plants that is used to treat peptic ulcer. The purpose of this study was to evaluate the effect of the addition of sodium carboxymethylcellulose (CMC) and sorbitol on anti-peptic ulcer activity of A. cordifolia leaves extracts in male Wistar rats. The plants were extracted using decoction method and freeze dried. Three liquid formulas were used i.e., i) a combination of sodium CMC and sorbitol; ii) only sorbitol; iii) extract only. The rats were divided into 6 groups, i.e., positive control (sucralfate 35 mg/kg body weight); negative control (80% ethanol); normal control; and 3 formulas. After the administration of the liquid formula, all groups, except normal control, were given 80% ethanol (l5 ml/kg body weight) to induce peptic ulcer. Antipeptic ulcer activity was evaluated using direct observation on rats gastric mucosa, and histopathology assessment. The result showed that the strongest anti-peptic ulcer  was shown by sorbitol only (96.95% inhibition),  followed by the combination of sodium CMC and sorbitol (92.68% inhibition). The formula which only contained extract showed only  31.70% inhibition.  Statistical analysis showed significant differences between formula 1 and 2 with negative controls. In conclusion, A. cordifolia leaves extract with the addition of sorbitol showed the strongest anti-peptic ulcer activity. Keyword: Anredera cordifolia, peptic ulcer, suspense, Wistar rat.

scholarly journals ECG DISPERSION MAPPING PARAMETERS DURING THE TREADMILL TEST IN PATIENTS WITH CORONARY HEART DISEASE

2013 ◽  
Vol 12 (2) ◽  
pp. 41-45
Author(s):  
G. G. Ivanov ◽  
E. V. Agafoshina ◽  
A. A. Pecherskikh ◽  
G. Khalabi
Keyword(s):  
Coronary Heart Disease ◽  
Heart Disease ◽  
Complex Analysis ◽  
Stress Test ◽  
Signs And Symptoms ◽  
Physical Stress ◽  
Functional Class ◽  
Control Group ◽  
Physiological Status ◽  
Treadmill Test

Aim.The aim of the study was the complex analysis of the ECG-12 and ECG dispersion mapping (DM) parameters during the physical stress test in patients with coronary heart disease (CHD).Material and methods.The main group included 143 CHD patients with Functional Class I–III angina (99 men and 44 women; mean age 50,6±4,6 years). The control group included 37 healthy individuals (mean age 44,3±4,8 years). Patients with heart failure signs and symptoms were excluded. All participants underwent the graduated physical stress test.Results.Two variants of the ECG changes were observed, with normal and abnormal reaction of ECG DM parameters. The latter group was characterised by the following: ratio of heart rate and myocardial micro-alternation index (HRmax/MMImax) 30% at the peak HR.Conclusion.Using the ECG DM method during the treadmill test is a feasible and useful approach to gain additional diagnostic information on the myocardial electro-physiological status and, therefore, to select the patients who need further examination and treatment. 

scholarly journals EFFECT OF Aloe vera GEL ON SUPEROXIDE DISMUTASE (SOD) LEVEL IN STREPTOZOTOCIN (STZ)-INDUCED DIABETIC WISTAR Rattus novergicus LIVER

2015 ◽  
Vol 2 (1) ◽  
pp. 51
Author(s):  
Nia Lukita Ariani ◽  
Abdul Gofur ◽  
Dwi Listyorini ◽  
Hendra Susanto ◽  
Yuda Handaya
Keyword(s):  
Diabetes Mellitus ◽  
Superoxide Dismutase ◽  
Aloe Vera ◽  
Wistar Rat ◽  
Positive Control ◽  
Negative Control ◽  
Diabetic Rats ◽  
Wistar Strain ◽  
Aloe Vera Gel ◽  
One Way Anova

Diabetes mellitus is a metabolic disorder characterized by the increasing levels of blood glucose as a result of either impaired of insulin secretion, insulin action, or both. Persistent hyperglycemia causes oxidative stress. Aloe vera is known of having an antioxidant activity. One of intrinsic antioxidant enzyme is superoxide dismutase (SOD) which eliminates superoxide radical. This research aims to determine the effect of Aloe vera gel on SOD level in STZ-induced diabetic Wistar rat liver. Wistar strain of Rattus novergicus used in this research were grouped into eight groups, consist of negative control (K-), non diabetic groups (NDM) consist of NDM 1 (dose of 30 mg/day), NDM 2 (dose of 60 mg/day), NDM 3 (dose of 120 mg/day), positive control (DM), and the DM group consists of DM 1 (dose of 30 mg/day), DM 2 (dose of 60 mg/day), and DM 3 (dose of 120 mg/day), with three replications each. Diabetic rats were induced using an intra-peritoneal injection of 60 mg/kg BW STZ. Three milliliters of Aloe vera gel were given intragastrointestinally for 14 days started from three days after injection of STZ. The level of liver SOD was measured with spectrophotometer.The results of One-Way Anova showed that Aloe vera gel has a significant effect on SOD levels (p< 0.05). Further analysis using LSD showed that only the treatment of NDM with 60 mg/day of Aloe vera gel giving a significant decreasing of SOD level compared to (K-). The conclusion is that Aloe vera has potency as a natural antioxidant. Keywords: Aloe vera, diabetes mellitus, superoxide dismutase (SOD), liver, streptozotocin (STZ) 

scholarly journals ECG DISPERSION MAPPING PARAMETERS DURING THE TREADMILL TEST IN PATIENTS WITH CORONARY HEART DISEASE

2013 ◽  
Vol 12 (2) ◽  
pp. 41-45
Author(s):  
G. G. Ivanov ◽  
E. V. Agafoshina ◽  
A. A. Pecherskikh ◽  
G. Khalabi
Keyword(s):  
Coronary Heart Disease ◽  
Heart Disease ◽  
Complex Analysis ◽  
Stress Test ◽  
Signs And Symptoms ◽  
Physical Stress ◽  
Functional Class ◽  
Control Group ◽  
Physiological Status ◽  
Treadmill Test

Aim. The aim of the study was the complex analysis of the ECG-12 and ECG dispersion mapping (DM) parameters during the physical stress test in patients with coronary heart disease (CHD).Material and methods. The main group included 143 CHD patients with Functional Class I–III angina (99 men and 44 women; mean age 50,6±4,6 years). The control group included 37 healthy individuals (mean age 44,3±4,8 years). Patients with heart failure signs and symptoms were excluded. All participants underwent the graduated physical stress test.Results. Two variants of the ECG changes were observed, with normal and abnormal reaction of ECG DM parameters. The latter group was characterised by the following: ratio of heart rate and myocardial micro-alternation index (HRmax/MMImax) 30% at the peak HR.Conclusion. Using the ECG DM method during the treadmill test is a feasible and useful approach to gain additional diagnostic information on the myocardial electro-physiological status and, therefore, to select the patients who need further examination and treatment. 

scholarly journals MicroRNA-21 Negatively Regulates Treg Cells Through a TGF-β1/Smad-Independent Pathway in Patients with Coronary Heart Disease

2015 ◽  
Vol 37 (3) ◽  
pp. 866-878 ◽  
Author(s):  
Sihui Li ◽  
Qian Fan ◽  
Shaolin He ◽  
Tingting Tang ◽  
Yuhua Liao ◽  
...  
Keyword(s):  
Coronary Heart Disease ◽  
Heart Disease ◽  
Mononuclear Cells ◽  
Inflammatory Diseases ◽  
Pain Syndrome ◽  
Treg Cells ◽  
Protective Role ◽  
Negative Control ◽  
Peripheral Blood Mononuclear ◽  
Tgf Β1

Background: CD4+CD25+FoxP3+ regulatory T cells (Treg cells) play a protective role against the development and progression of the inflammatory disease atherosclerosis (AS). MicroRNA-21 (miR-21) is expressed in Treg cells and is up-regulated in the context of AS and other inflammatory diseases. Aims: This study aimed to determine the role of miR-21 in Treg cell regulation and gene expression during the development of AS in patients with coronary heart disease (CHD). Methods and Results: MiR-21 expression in peripheral blood mononuclear cells (PBMCs) was significantly up-regulated in patients with CHD (acute myocardial infarction (AMI) group, n=24; unstable angina (UA) group, n=21; stable angina (SA) group, n=24) compared with patients with chest pain syndrome (CPS, n=27), and miR-21 expression showed an increasing trend from SA to UA to AMI patients. Moreover, flow cytometry analysis indicated that the frequencies of circulating Treg cells decreased in a manner proportionate opposite with the level of miR-21. Quantitative real-time PCR (qRT-PCR) revealed a decrease in mRNA expression of forkhead box P3 (foxp3), transforming cell growth factor beta 1(TGF-β1) and smad7 (a known target gene of miR-21). ELISA analysis revealed a decrease in TGF-β1 secreted into the plasma. In addition, we transfected PBMCs with a miRNA negative control (NS-m), a miR-21 mimic (miR-21-m), a miRNA inhibitor negative control (NS-i), or a miR-21 inhibitor(miR-21-i). Up-regulation of miR-21 decreased the frequency of circulating Treg cells, decreased the expression levels of foxp3, TGF-β1 and smad7, and decreased the amount of TGF-β1 secreted into the plasma. Consistent with these observations, miR-21 down-regulation increased the frequency of circulating Treg cells, increased the expression of foxp3, TGF-β1 and smad7, and increased the amount of TGF-β1 secreted into the plasma. Conclusions: Because the smad7 expression pattern was similar to that of TGF-β, our study suggests that miR-21 can negatively regulate the frequency of circulating Treg cells through a TGF-β1/smad-independent signaling pathway in PBMCs.

Effect of short-term growth of mung bean and its soil incorporation on the density of the soybean cyst nematode, Heterodera glycines, in pot experiments

Nematology ◽  
2017 ◽  
Vol 19 (10) ◽  
pp. 1147-1155 ◽  
Author(s):  
Seiya Chikamatsu ◽  
Wang Xiaoman ◽  
Daisuke Ito ◽  
Emi Yamada ◽  
Koki Toyota
Keyword(s):  
Vigna Radiata ◽  
Mung Bean ◽  
Soybean Cyst Nematode ◽  
Heterodera Glycines ◽  
Cyst Nematode ◽  
Raw Material ◽  
Short Term ◽  
Bean Sprout ◽  
Dry Soil ◽  
Moisture Conditions

We previously reported that incorporation of bean sprout residue into soil stimulated hatching of the soybean cyst nematode (SCN), Heterodera glycines. The objective of this study was to evaluate the effects of short-term growth of mung bean (Vigna radiata), the raw material of bean sprout, and its incorporation into the soil on the density of SCN in soil. When mung bean was grown in pots for 2 and 4 weeks in a SCN-infested andosol, incorporated into the soil and further incubated for 2 weeks, the SCN density decreased markedly. Mung bean was also grown in pots under different moisture conditions. The SCN density decreased in the soils with 48-68 g water, but not with 39 g water (100 g dry soil)−1 in which mung bean grew the poorest. The present study suggests that short-term growth of mung bean may be useful to decrease the density of SCN in soil.

scholarly journals PENGARUH PEMBERIAN EKSTRAK KUNYIT TERHADAP GAMBARAN HISTOPATOLOGI AORTA TIKUS WISTAR (rattus novergicus) HIPERLIPIDEMIA

2015 ◽  
Vol 3 (1) ◽  
Author(s):  
Anggelina Thendry ◽  
Lily L. Loho ◽  
Poppy M. Lintong
Keyword(s):  
Wistar Rat ◽  
Foam Cells ◽  
Negative Control Group ◽  
Rat Aorta ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Turmeric Extract ◽  
Group B

Abstract: Atherosclerosis is a chronic inflammatory process that can be triggered by one of the risk factors of hyperlipidemia. This research was conducted to see the aorta associated with the provision of margarine that induce hyperlipidemia and administration of curcumin turmeric-containing compounds that act as antioxidants and anti-inflammatory. This research is experimental. The purpose of this study to determine the effect of extract of turmericon Wistar rat aorta histopathology induced hyperlipidemia with margarine. This study used 20 Wistar rats were divided into 5 groups, each group consisting of 4 rats. Group A (negative control group) is given only standard pellets, group B (positive control group) was given margarine 5gr/head /dayfor 28 days, group C was given margarine 5gr/head /day for 28 days followed by administration of standard pellets for 7 day, group D given margarine 5gr/head/day for 28 days followed by administration of turmeric extract 50 mg /head/day for 7 days, and group E are given margarine 5gr/head/day along with turmeric extract 50 mg/head/dayfor 28days. The results shows that microscopically visible aortic wall with foam cells in the tunica intima and tunica media in the positive control group and the administration of 50 mg of turmeric extract reduced foam cells. Conclusion: Turmeric extract showed foam cells in the intima and media less than that of margarine.Keywords: hyperlipidemia,turmeric, margarine, aorta histopathologyAbstrak: Aterosklerosis adalah proses radang kronik yang dapat dicetuskan oleh salah satu faktor resiko hiperlipidemia. Penelitian ini dilakukan untuk melihat pembuluh darah aorta berhubungan dengan pemberian margarin yang menginduksi hyperlipidemia dan pemberian kunyit yang mengandung senyawa kurkumin yang berperan sebagai antioksidan dan anti inflamasi. Penelitian ini bersifat eksperimental.Tujuan penelitian ini untuk mengetahui pengaruh pemberian ekstrak kunyit terhadap gambaran histopatologi aortatikus wistar hiperlipidemia yang diinduksi dengan margarin. Penelitian ini menggunakan 20 ekor tikus wistar yang dibagi ke dalam 5 kelompok yang masing-masing kelompok terdiri dari 4 ekor tikus. Kelompok A(kelompok kontrol negatif) hanya diberikan pelet standar, kelompok B(kelompok kontrol positif) diberikan margarin 5gr/ekor/hari selama 28 hari, kelompok C diberikan margarin 5gr/ekor/hari selama 28 hari kemudian dilanjutkan dengan pemberian pelet standar selama 7 hari, kelompok D diberikan margarin 5gr/ekor/hari selama 28 hari kemudian dilanjutkan dengan pemberian ekstrak kunyit 50mg/ekor/hari selama 7 hari, dan kelompok E yang diberikan margarin 5gr/ekor/hari bersamaan dengan pemberian ekstrak kunyit 50mg/ekor/hari selama 28 hari. Secara mikroskopik tampak dinding aorta dengan sel-sel busa pada tunika intima dan tunika media pada kelompok kontrol positif dan pada pemberian ekstrak kunyit 50 mg sel busa berkurang. Simpulan: Pemberian ekstrak kunyit menunjukan sel-sel busa pada tunika intima dan media lebih sedikit dibandingkan dengan pemberian margarin.Kata kunci: hiperlipidemia, kunyit, margarin, istopatologi aorta

scholarly journals Short Communication: Hepatoprotective effect of methanol extract of sambiloto leaves (Andrographis paniculata) against malondialdehyde levels in liver tissues of paracetamol-induced Wistar rat

2018 ◽  
Vol 10 (2) ◽  
pp. 87-90
Author(s):  
ANDRIANI ANDRIANI ◽  
VIRHAN NOVRIANY ◽  
GUNTUR SUSENO ◽  
EFFIANA EFFIANA ◽  
IIT FITRIANINGRUM
Keyword(s):  
Normal Control ◽  
Short Communication ◽  
Methanol Extract ◽  
Wistar Rat ◽  
Positive Control ◽  
Negative Control ◽  
Andrographis Paniculata ◽  
Hepatoprotective Effect ◽  
Control Group ◽  
Liver Tissues

Andriani, Suseno G, Effiana, Novriany V, Fitrianingrum I. 2018. Short Communication: Hepatoprotective effect of methanol extract of sambiloto leaves (Andrographis paniculata) against malondialdehyde levels in liver tissues of paracetamol-induced Wistar rat. Nusantara Bioscience 10: 87-90. Andrographis paniculata contains andrographolide and flavonoids, which have a hepatoprotective effect. This study was aimed to examine hepatoprotective effect of Andrographis paniculata extract against liver tissues malondialdehyde (MDA) levels of paracetamol induced Wistar rat (Rattus novegicus). The study was designed with posttest-only control group conducted at Tanjungpura University, Indonesia in June 2015. A total of 30 rats at age of three weeks were divided into 6 groups. They were given toxic dose paracetamol, curcumin and A. paniculata extract in different dose. Liver tissues were tested by Wills’s method. The data was analyzed using One-way ANOVA and LSD Post-hoc test. MDA levels of liver tissues were i.e, normal control (0.34 nmol mL-1), positive control (0.34 nmol mL-1), dose 1 (0.45 nmol mL-1), dose 2 (0.55 nmol mL-1), dose 3 (0.63 nmol mL- 1 ), negative control (1.32 nmol mL-1). Result showed that MDA levels of liver tissues of negative control group were significantly different with other groups (ANOVA, p≤ 0.05). There were no significant differences between MDA levels of liver tissues of Dose 1 with normal control and positive control (ANOVA, p>0.05). The methanol extract of A. paniculata has a hepatoprotective effect in (MDA) levels of paracetamol-induced Rattus novegicus with an effective dose of 500 mg kg-1.

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