Mung bean sprout extract suppresses Monosodium Glutamate (MSG) effect  on the reproductive hormones (FSH and Estrogen) in female Wistar rats

Objectives: The purpose of this study was to analyze the effects of mung bean sprout extracts on Follicle stimulating hormone (FSH) and estrogen hormone in female Wistar rats (Rattus norvegicus) exposed to monosodium glutamate (MSG).Materials and Methods: This true experimental study was conducted by using post-test only control group design in the laboratory for animal experimentation of Faculty of Veterinary Medicine - Airlangga University in which Wistar rats (Rattus norvegicus) aged 2 months weighing 150-200 grams were used in this experiment. The samples comprised of 5 rats distributed in each group, totaling 7 groups. I Control Group (P1) was provided with Aquades for 37 days; II (P2) was provided with Aquades for 7 days + MSG 0.03 mg/g of weight on day 8-37; III (P3) was provided with extract of mung bean sprouts 72mg/200g of weight on day 1-37 + MSG 0,03mg/g of weight; IV (P4) was provided with extract of mung bean sprouts 144mg/200g of weight on day 1-37 + MSG 0.03mg/g of weight; V (P5) was provided with Aquades for 7 days + MSG 0.7 mg/g of weight on day 8-37; VI (P6) was provided with extract of mung bean sprouts 72mg/200g of weight on day 1-37 + MSG 0.7mg/g of weight; VII (P7) was provided with extract of mung bean sprouts 144mg/200g of weight on day 1-37 + MSG 0.7mg/g of weight.Serum samples were taken for Follicle Stimulating Hormone (FSH) and Estrogen with ELISA method. Data analysis to test the differences between groups was done by using one way Anova statistical test.Results: MSG dosage 0,03mg/gBB or 0,7mg/g BB was significant difference in FSH (p = 0,011) and estrogen (p = 0,008).Conclusion: obtained from this research that giving green bean sprout extract influence to the level of FSH and estrogen hormone.

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Effects of Oral Administration of Monosodium Glutamate (MSG) on Obesity in Male Wistar Rats (Rattus Norvegicus)

BioScientia Medicina ◽

10.32539/bsm.v5i9.355 ◽

2021 ◽

Vol 5 (9) ◽

pp. 879-882

Author(s):

Mulyati Sri Rahayu ◽

Sri Wahyuni ◽

Yuziani

Keyword(s):

Body Weight ◽

Adult Male ◽

Rattus Norvegicus ◽

Wistar Rats ◽

Body Weight Gain ◽

Monosodium Glutamate ◽

Control Group ◽

Significant Difference ◽

Male Wistar Rats ◽

Group 2

Introduction: Monosodium glutamate (MSG) is one of the most widely employed food enhancers. Although the umami compound, controversy persists regarding the effects of MSG intake on body weight. Chronic MSG intake may result in excessive body weight gain and obesity. Consumption of MSG result in organ damage, cardiovascular disease, oxidative stress, and also risk factors for obesity. This study aims to determine the effect of oral MSG on obesity in adult male Wistar rats (Rattus norvegicus).Methods: This true experimental study used the post-test control group design. Twenty-four adult male Wistar rats were randomly divided into four groups: control (received distilled water), Group 1 (MSG 0.378 mg/gr BW), Group 2 (0.756 mg/gr BW) and Group 3 (1.512 mg/gr BW). The obesity parameter was obtained by the Lee index. Kruskal-Wallis test follows by Mann-Whitney test were used to compare the Lee index between groups.Results: Lee’s index mean for each group was 358.4%, 314.1%, 287.8%, and 320.9%, respectively. The Kruskal Wallis test showed a significant difference in the Lee index between groups (p = 0.043). A follow-up test using Mann-Whitney found a significant difference between group 2 and the control group (p = 0.043, p <0.05). The mean of Lee index of group 2 was 70.51% lower than the control group.Conclusion: This study concluded that Lee index was not increased in MSG-treated rats than in the control group after oral MSG intervention for 21 days.

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Pemberian suplemen Swissoats A3® meningkatkan kadar hormon estrogen pada tikus (Rattus norvegicus) betina menopause galur Wistar

JURNAL BIOMEDIK (JBM) ◽

10.35790/jbm.9.2.2017.16354 ◽

2017 ◽

Vol 9 (2) ◽

Author(s):

Trisna A. Widhayanthi ◽

Wimpie Pangkahila ◽

Gde Ngurah Indraguna Pinatih

Keyword(s):

Rattus Norvegicus ◽

Wistar Rats ◽

Indirect Elisa ◽

Control Group ◽

Medial Canthus ◽

Control Group Design ◽

Group Design ◽

Estrogen Level ◽

Female Wistar Rats ◽

The Mean

Abstract: Aging is a physiological process that occurs in all living creatures. Swissoats A3® is an anti-aging supplement that contains phytoestrogens. This study was aimed to prove that Swiss oats A3® supplementation could increase estrogen levels in menopausal female Wistar rats (Rattus norvegicus). This was an experimental study with a completely randomized pretest-posttest control group design. Subjects were 20 menopausal female rats (Rattus norvegicus), Wistar strain, healthy, aged 15-16 months, weighing 200 g, divided into 2 groups: the control group (P0), treated with distilled water for 21 days and the treatment group (P1), treated with Swissoats A3® of 34.2 mg/rat/day for 21 days. Before and after 21 days of treatment, 1 ml venous blood was drawn through the medial canthus of orbital sinus using microcapillary tube and the estrogen levels were meassured by using indirect ELISA method. The results showed that before treatment, the mean estrogen level in P0 group was 14.96±2.0468 pg/ml and in P1 group was 14.21±2.3905 pg/ml (P > 0.05). Meanwhile, after 21-day treatment, the mean estrogen level in P0 group was 15.07±1.8851 pg/ml, whereas in P1 group was 28.33±3.2052 pg/ml (P < 0.01). The analysis showed that the Swissoats A3® could elevate the estrogen levels significantly (P < 0.01) in group P1 (2.3905±14.21 vs 28.33±3.205). Conclusion: Swissoats A3® supplementation could increase the estrogen levels in menopausal female Wistar rats (Rattus norvegicus).Keywords: Swiss oats A3®, estrogen, female Wistar rats, menopauseAbstrak: Penuaan (aging) merupakan suatu proses fisiologik yang dialami oleh seluruh mahluk hidup. Swissoats A3® merupakan suplemen anti-aging yang mengandung fitoestrogen. Penelitian ini bertujuan untuk membuktikan bahwa pemberian suplemen Swissoats A3® dapat meningkatkan kadar estrogen pada tikus (Rattus norvegicus) betina menopause galur Wistar. Jenis penelitian ialah eksperimental dengan randomized pretest-posttest control group design. Subjek penelitian ialah 20 ekor tikus putih betina (Rattus norvegicus) galur Wistar, sehat, menopause, umur 15-16 bulan, berat badan 200 gr. Tikus dibagi menjadi 2 kelompok yaitu kelompok kontrol (P0) yang diberikan aquadest secara sonde selama 21 hari dan kelompok perlakuan yang diberikan suplemen Swissoats A3® dosis 34,2mg/ekor/hari secara sonde selama 21 hari. Sebelum dan sesudah 21 hari perlakuan, darah vena diambil melalui medial canthus sinus orbitalis sekitar 1 ml dengan menggunakan tabung mikrokapiler dan dilakukan pemeriksaan kadar estrogen menggunakan metode indirect ELISA. Hasil penelitian menunjukkan bahwa sebelum perlakuan, rerata kadar estrogen kelompok P0 14,96±2,0468 pg/ml dan kelompok P1 14,21±2,3905 pg/ml (P > 0,05). Setelah perlakuan selama 21 hari, rerata kadar estrogen kelompok P0 15,07±1,8851 pg/ml dan kelompok P1 28,33±3,2052 pg/ml (P < 0,01). Analisis efek perlakuan menunjukkan terjadinya peningkatan kadar hormon estrogen yang sangat bermakna (P < 0,01) pada kelompok P1 (14,21±2,3905 vs 28,33±3,205). Simpulan: Swissoats A3® dapat meningkatkan kadar estrogen pada tikus (Rattus norvegicus) betina menopause galur wistar.Kata kunci: Swissoats A3®, estrogen, tikus wistar betina, menopause

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Peran kecambah kacang hijau (Phaseolus radiatus (L.)) pada sel lemak retroperitoneal tikus Sprague Dawley yang diberi diet tinggi lemak

Jurnal Gizi Klinik Indonesia ◽

10.22146/ijcn.41676 ◽

2019 ◽

Vol 16 (1) ◽

pp. 31

Author(s):

Dwi Lestari ◽

Wiryatun Lestariana ◽

Lily Arsanti Lestari ◽

Arta Farmawati

Keyword(s):

Vitamin E ◽

Mung Bean ◽

Feeding Tube ◽

Cell Number ◽

Cell Diameter ◽

Sprague Dawley ◽

High Fat ◽

Bean Sprout ◽

Mung Bean Sprouts ◽

Bean Sprouts

The role of mung bean sprouts (Phaseolus radiatus (L.)) on retroperitoneal fat cells of Sprague Dawley rats fed a high-fat dietBackground: The tendency of current lifestyles with high consumption patterns of fat is one of the factors causing obesity which is a risk of cardiovascular disease. Prevention of cardiovascular disease can be done by adopting a dietary pattern that is rich in antioxidants including consumption of sources of vitamin E. Sprouts from mung beans contain vitamin E and phytochemicals rich in antioxidants, so they can be used as an alternative to prevent hyperlipidemia sourced from daily functional food.Objective: To assess the effects of mung bean sprouts on retroperitoneal fat weight, cell number and cell diameter in rats fed high-fat feed.Method: This research is an experimental study with a post-test design with the control group. Thirty-four male Sprague Dawley rats ± 2 months old were divided into five groups, group I (standard feed), group II (high-fat feed), group III (high-fat feed and intervention of feeding tube of mung bean sprout 0.67 g/ 200 g BW), group IV (high fat feed and intervention of feeding tube of mung bean sprout 1.34 g / 200 g BW), and group V (high-fat feed and 23 IU dose of vitamin E supplement with feeding tube). After adaptation for three days, the intervention was carried out for four weeks. Analysis of fat weight, fat cell number, and fat cell diameter were done after the treatments. Data analysis using the One-Way ANOVA test. Results: Mung bean sprout dose of 1.34 g given for 4 weeks did not significantly decrease fat weight and cell number, but it decreased the diameter of retroperitoneal fat cells.Conclusion: Mung bean sprouts dose 1.34 g was better than a dose of 0.67 g and vitamin E supplementation in reducing the diameter of retroperitoneal fat cells in rats fed a high-fat diet.

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The Effect of Propofol on Onset and Sedation in Diabetic Mellitus Rat (Rattus norvegicus)

Jurnal Medika Veterinaria ◽

10.21157/j.med.vet..v8i2.3354 ◽

2014 ◽

Vol 10 (2) ◽

Author(s):

Erwin Erwin ◽

Rusli Rusli ◽

Zuraidawati Zuraidawati ◽

Fadillah Irwansyah

Keyword(s):

Blood Glucose ◽

Rattus Norvegicus ◽

Wistar Rats ◽

Control Group ◽

Diabetic Mellitus ◽

Wistar Strain ◽

Female Wistar Rats ◽

Ad Libitum ◽

Group 2 ◽

Group 1

This research aimed to examine the time of onset and sedation on diabetic mellitus (DM) rat (Rattus norvegicus) by propofol. This study used 8 female wistar rats 2-3 months old and 150-200 grams of bodyweight, fed with standard feed and water ad libitum. Samples were divided into two groups. Group 1 (KI) as a control group was injected intraperitoneally with aloxan solvent and group 2 (KII) was injected intraperitoneally with 150 mg/kg bodyweight aloxan. Blood glucose was checked 10 days after aloxan injection, DM was categorized if the blood glucose over 150 mg/dl. All rats were injected intravenously with propofol 1% with the dose of 10 mg/kg bodyweight. Onset and sedation time was counted using stopwatch after propofol injection. The result showed that mean (±SD) of onset on KI and KII were 1.39±0.49 and 8.64±1.23 seconds, consecutively and they were significantly different at P0.01. Whilst mean (±SD) of sedation KI and KII were 12.12±1.47 minutes and 7.62±1.61 minutes, respectively. The onset and sedation time of KI was significantly different from KII P0.01. The conclusion of this research was DM affects time of onset and sedation when wistar strain rats were anesthetized by 1% propofol.Key words: rats, diabetic mellitus, propofol, onset, sedation

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Pengaruh pemberian kecambah kacang hijau (Phaseolus radiatus (L.)) terhadap kadar malondealdehid (MDA) plasma dan jaringan hati tikus Sprague Dawley yang diberi pakan lemak tinggi

Jurnal Gizi Klinik Indonesia ◽

10.22146/ijcn.22923 ◽

2016 ◽

Vol 13 (2) ◽

pp. 82

Author(s):

Novidiyanto Novidiyanto ◽

Arta Farmawati ◽

Lily Arsanti Lestari

Keyword(s):

Lipid Peroxidation ◽

Plasma Level ◽

High Fat Diet ◽

Mung Bean ◽

Liver Tissue ◽

Control Group ◽

Sprague Dawley ◽

High Fat ◽

Mung Bean Sprouts ◽

Bean Sprouts

Background: High-fat diet increases the levels of fat, especially cholesterol and triglycerides that cause hyperlipidemia. Polyunsaturated fatty acids are very easily oxidized by free radicals reactive oxygens species (ROS) called lipid peroxidation, to produce compounds that malondialdehyde (MDA), is toxic and can damage plasma membrane and liver tissue. Mung bean sprouts are known to contain antioxidants which act to inhibit lipid peroxidation process.Objective: Determine the effect of mung bean sprouts to the level of plasma MDA and liver tissue of rats with high-fat diets.Method: This research is an experimental study with an only post-test design with the control group. Thirty-five male Sprague-Dawley rats were divided into 5 groups. Group K1 received fed standard; K2 received high-fat diet without mung bean sprouts. Group P1 received high-fat diet and mung bean sprouts dose of 0.5 mL/gBW. Group P2 received high-fat diet and mung bean sprouts dose of 1 mL/gBW. Group P3 received high-fat diet and vitamin E doses of 23 IU. All group were treated for 28 days. Analysis of MDA plasma and liver tissue performed after treatment.Results: Group K1 has plasma MDA levels of 1.17 ± 0.14 nmol/mL Mean MDA plasma level in the group K1 Group of K1 Mean MDA plasma level in the group K2 (39.7±0.19 nmol/mL) was higher than the group of K1 (1,17±0,14 nmol/mL), group P1 (2.94±0.09 nmol/mL), group P2 (1.73±0.08 nmol/mL) and group P3 (1.53±0.07 nmol/mL). Group K1 has liver tissue MDA Levels of 1,64±0,11 nmol/g. Mean MDA liver tissue level in the group of K2 (4.90±0.29 nmol/g) was higher than the group of P1 (3.68±0.45 nmol/g), group P2 (2.27±0.10 nmol/g), and group P3 (2.01±0.11 nmol/g).Conclusion: Level of MDA plasma and liver tissue of rat in group P1 and P2 was lower than a level of MDA plasma and liver tissue of rat in group K1, but higher than the level of MDA plasma and liver tissue of rat in group P3. There was a very strong correlation between the level of MDA plasma and liver tissue of rat was given the mung bean sprouts and high-fat diet.

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PENGARUH KONSENTRASI KECAMBAH KACANG HIJAU TERHADAP SIFAT FISIK DAN KIMIA TEPUNG TALAS KIMPUL

Pro Food ◽

10.29303/profood.v6i1.135 ◽

2020 ◽

Vol 6 (1) ◽

pp. 634

Author(s):

Hariyadi Hariyadi ◽

Yeni Sulastri ◽

Zainuri Zainuri

Keyword(s):

Moisture Content ◽

Protein Content ◽

Mung Bean ◽

Chemical Properties ◽

Ash Content ◽

Bean Sprout ◽

Mung Bean Sprouts ◽

Taro Flour ◽

Significant Difference ◽

Bean Sprouts

ABSTRACT This study aims to find out the effect of mung bean sprout concentration on the physical and chemical properties of taro flour. The method used in this research was experimental method and was designed using Completely Randomized Design with a single factor experiment (the concentration of mung bean sprouts). There were 5 treatments (0%, 10%, 20%, 30% and 40% of mung bean sprouts), and each treatment were made into 4 replications. Data from the observations were analyzed using analysis of Variance (ANOVA) at 5% significant level using Co-Stat software. If there was significant difference, the data were further tested using the Honest Real Difference test at 5% significant level too. The results showed that the addition of mung bean sprout extract significantly affected the moisture content, protein content, ash content and oxalate content of taro flour. The treatment of 40% mung bean sprout extract is recommended as the best treatment for moisture content (10.53%), ash content (5.24%), protein content (3.84%), calcium oxalate content (0.0268%), water absorption (87.42%), and L value (88.14) of modified taro flour. Keywords: flour, taro, enzymes, mung bean sprouts ABSTRAK Tujuan penelitian ini adalah untuk mengetahui pengaruh konsentrasi kecambah kacang hijau terhadap sifat fisik dan kimia tepung talas kimpul. Metode yang digunakan dalam penelitian ini adalah metode eksperimental dan dirancang menggunakan Rancangan Acak Lengkap (RAL) dengan faktor tunggal (konsentrasi kecambah kacang hijau). Terdapat 5 perlakuan (konsentrasi 0%, 10%, 20%, 30% dan 40% kecambah kacang hijau), dan tiap perlakuan dibuat 4 ulangan. Data hasil pengamatan dianalisis dengan menggunakan analisis keragaman pada taraf nyata 5% dengan menggunakan software Co-Stat. Apabila terdapat beda nyata, maka akan diuji lanjut menggunakan uji Beda Nyata Jujur (BNJ) pada taraf 5% juga. Hasil penelitian menunjukkan bahwa penambahan ekstrak kecambah kacang hijau berpengaruh nyata terhadap kadar air, kadar protein, kadar abu dan kadar oksalat tepung talas. Perlakuan konsentrasi ekstrak kecambah kacang hijau 40% direkomendasikan sebagai perlakuan terbaik, yang menghasilkan tepung talas dengan karakteristik sebagai berikut: kadar air (10,53%), kadar abu (5,24%), kadar protein (3,84%), kadar kalsium oksalat (0,0268%), daya serap air (87,42%), dan nilai L (88,14) tepung talas termodifikasi. Kata kunci: tepung, talas, enzim, kecambah kacang hijau

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Effects of Oral Administration of Monosodium Glutamate (MSG) on Obesity in Male Wistar Rats (Rattus Norvegicus)

BioScientia Medicina ◽

10.32539/bsm.v5i4.355 ◽

2021 ◽

Vol 5 (4) ◽

pp. 851-854

Author(s):

Mulyati Sri Rahayu ◽

Sri Wahyuni ◽

Yuziani

Keyword(s):

Body Weight ◽

Adult Male ◽

Rattus Norvegicus ◽

Wistar Rats ◽

Body Weight Gain ◽

Monosodium Glutamate ◽

Control Group ◽

Significant Difference ◽

Male Wistar Rats ◽

Group 2

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Pencegahan hipertensi dan penebalan dinding aorta dengan pemberian kecambah kacang hijau (Phaseolus radiatus (L)) pada tikus putih Sprague Dawley

Jurnal Gizi Klinik Indonesia ◽

10.22146/ijcn.22454 ◽

2016 ◽

Vol 12 (3) ◽

pp. 116

Author(s):

Novian Swasono Hadi ◽

Arta Farmawati ◽

Ahmad Ghozali

Keyword(s):

Blood Pressure ◽

Vitamin E ◽

High Fat Diet ◽

Mung Bean ◽

Male Rats ◽

Sprague Dawley ◽

High Fat ◽

Bean Sprout ◽

Mung Bean Sprouts ◽

Bean Sprouts

Background: Lifestyle changes with high-fat food consumption is one of the factors the risks of cardiovascular diseases like of coronary heart disease and atherosclerosis. A healthy diet and a balanced diet and consume foods that contain lots of antioxidants is one of the effective ways to prevent hyperlipidemia. Mung bean sprouts have properties that neutralize free radicals cause Hyperlipidemia and cardiovascular diseases because it is an antioxidant compound.Objective: The aim of this study was to determinate the effect of mung bean sprouts (Phaseolus radiatus (L)) to blood pressure and histopathology aorta of Sprague-Dawley male rats.Method: The type of study was experimental research using pre-post test controlled group design for blood pressure variable and post test only controlled group design histopathology aorta. The thirty-five of Sprague-Dawley male rats was eight weeks divided into 5 groups. The first group was given standard diet, group 2 was given a hight fat diet, the third group was given a high-fat diet and mung bean sprout 0,67 gram, group 4 was given a high-fat diet and mung bean 1,34 gram, and group 5 was given a high-fat diet and vitamin E doses of 23 IU.Results: Result of this study showed that after 4 weeks of treatment, increased in blood pressure systole in the given of high fat diet higher than group who were given a high fat diet and mung bean sprout and also on group who were given high fat diet and vitamin E, but there is no difference effect a decrease in blood pressure between the provision of mung bean sprouts and vitamin E (p>0,05). Statistical analysis to thick the wall the aorta show the similarity meaningful in all the treatment group, it can be said that overall thick the wall the aorta in this research is not different.Conclusion: A dose of mung bean sprout 0,67 g is optimal doses in preventing a rise in blood pressure and prevent alterations histopathology Sprague-Dawley male rats.

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Evaluation of Commercial Kit Based on Loop-Mediated Isothermal Amplification for Rapid Detection of Low Levels of Uninjured and Injured Salmonella on Duck Meat, Bean Sprouts, and Fishballs in Singapore

Journal of Food Protection ◽

10.4315/0362-028x.jfp-14-535 ◽

2015 ◽

Vol 78 (6) ◽

pp. 1203-1207 ◽

Cited By ~ 11

Author(s):

HAZEL SIN YUE LIM ◽

QIANWANG ZHENG ◽

MARTA MIKS-KRAJNIK ◽

MATTHEW TURNER ◽

HYUN-GYUN YUK

Keyword(s):

Validation Study ◽

Mung Bean ◽

Isothermal Amplification ◽

International Organization ◽

Standardization Method ◽

Bean Sprout ◽

Mung Bean Sprouts ◽

Secondary Enrichment ◽

Commercial Kit ◽

Bean Sprouts

The objective of this study was to evaluate performance of the commercial kit based on loop-mediated isothermal amplification (LAMP) in comparison with the International Organization for Standardization method for detecting uninjured and sublethally injured Salmonella cells artificially inoculated at levels of 100 and 101 CFU/25 g on raw duck wing, raw mung bean sprouts, and processed fishballs. Injured cells were prepared by a heat treatment for duck wings and fishball samples and a chlorine treatment for bean sprout samples. Additionally, a validation study was performed on naturally contaminated food samples sold in Singapore. A total of 110 samples of each commodity were analyzed in this study. Regardless of inoculum levels, the detection by the commercial LAMP kit showed 100% sensitivity and specificity for both inoculated and uninoculated samples compared with the International Organization for Standardization method, with the exception of bean sprout samples. Only 20% of bean sprout samples inoculated with 100 CFU/25 g injured Salmonella cells were positive by using the commercial LAMP-based kit. However, all negative samples became positive following a secondary enrichment in Rappaport-Vassiliadis medium with soy broth or after concentration by centrifugation. These results suggest that secondary enrichment or centrifugation should be considered as an additional step to increase the sensitivity of the commercial LAMP-based kit with low numbers of injured target cells in samples with high background microflora (such as mung bean sprouts). The validation study also showed that the commercial LAMP-based kit provided 91% sensitivity and 95% specificity for naturally contaminated samples. Thus, this study demonstrates that the commercial LAMP-based kit might be a cost-effective method, as this system could provide rapid, accurate detection of both uninjured and injured Salmonella cells on raw duck wings, raw mung bean sprouts, and processed fishballs in less than 26 h.

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