scholarly journals Breeding and Larval Rearing of Asian Moon Scallop Amusium pleuronectes in Eastern Samar, Philippines

2020 ◽  
Vol 27 (1) ◽  
Author(s):  
Nonita Cabacaba ◽  
Ed-Marie Boiser ◽  
Kimberly Badocdoc ◽  
Cristan Joy Campo

Asian moon scallop, Amusium pleuronectes, is among the most common commercially harvested scallop in the Philippines. This study investigated suitable conditioning methods, induced spawning, and documented scallop larval development at the Marine Fisheries Research and Development Center (MFRDC) Guiuan, Eastern Samar. Scallops held in tanks with flow-through water system without substrate yielded high survival rate of 48.06 ± 5.95%. Optimum water temperature was 28-29°C for maintaining scallops. Isochrysis galbana and Chaetoceros calcitrans were suitable microalgal feed for A. pleuronectes consumed at 7,388,888 cells min-1 per scallop. These significant results were applied in maintaining scallops at the hatchery. As a result, scallops with an average 19.8 mm shell length (SL) reached 37.5 mm SL within four months with a survival rate of 96.57 ± 2.04%, average daily growth rate (ADGR) of 0.13 ± 0.04 mm.day-1, and specific growth rate (SGR) of 3.92 ± 1.31%.d-1. Natural spawning was successful under controlled conditions while induced spawning trial through thermal stimulation, food shock, sexual stimulation, and serotonin injection resulted unsuccessful release of sperm and eggs. The estimated number of fertilized eggs per spawning ranged from 0.22-1.4 million. Fertilized eggs appeared spherical and dark in color with 54.2-62.57 µm in diameter. After nine hours, the larvae developed into trochophore stage with 59.08-84.4 µm in length. D-veliger with 120.37-157.07µm shell length developed after 24 hours. Development of the early umbone stage was reached on day 5; and on day 7, the umbo larvae become well-developed with shell length of 135.45-173.36 µm. On day 9, pedi-veligers were observed in the culture. Spat grew 312.41-509.48 µm on day 16 and survived until four months with final shell length of 4-10 mm. For the larval rearing, stocking density of A. pleuronectes larvae observed highest survival rate of 0.04 ± 0.03% at 200 larvae/L, while no larvae survived at 800 larvae/L due to contamination of protozoans in the culture medium.

2018 ◽  
Vol 13 (1) ◽  
pp. 39
Author(s):  
Fitriyah Husnul Khotimah ◽  
Gusti Ngurah Permana ◽  
Ibnu Rusdi ◽  
Bambang Susanto

Masalah utama yang umum terjadi pada produksi benih abalon adalah kematian yang tinggi (> 90%) setelah abalon menempel pada plate pemeliharaan. Penggunaan pakan dalam bentuk tepung untuk mengganti diatom sebagai pakan postlarva beberapa spesies ikan, udang, dan abalon sudah dilakukan. Penelitian ini bertujuan untuk mendapatkan jenis pakan dalam bentuk tepung yang sesuai dan efektif untuk mendukung sintasan dan pertumbuhan larva abalon Haliotis squamata. Percobaan terdiri atas lima perlakuan pakan pada pemeliharaan larva abalon yaitu tepung Spirulina sp., Ulva sp., Chaetoceros sp., Gracilaria sp., dan diatom (kontrol). Masing-masing perlakuan terdiri atas empat ulangan. Pakan berupa tepung yang digunakan pada masing-masing perlakuan, terlebih dahulu dicampur merata dengan larutan tepung agar (7,5 mg/mL dalam air laut; suhu 40°C) dengan konsentrasi tepung 40 mg/mL larutan agar. Pemberian pakan dilakukan setiap tiga hari dengan cara menyemprotkan larutan pakan pada permukaan plate pemeliharaan larva. Penelitian dilakukan selama 30 hari. Hasil penelitian menunjukkan bahwa sintasan larva abalon yang diberi pakan tepung Spirulina sp. paling tinggi dan berbeda nyata (P<0,05) dengan yang diberi diatom, tepung Chaetoceros sp., dan Ulva sp., yaitu masing-masing 81,49%; 79,25%; 76,57%; dan 76,46%; tetapi tidak berbeda nyata dengan yang diberi pakan tepung Gracilaria sp. 81,37% (P>0,05). Laju pertumbuhan harian panjang cangkang larva abalon tertinggi diperoleh pada larva yang diberi pakan tepung Gracilaria sp. (203,81 ± 1,23 µm/hari) dan Spirulina sp. (205,59 ± 1,71 µm/hari). Nilai laju pertumbuhan harian panjang cangkang larva abalon yang paling rendah dijumpai pada larva yang diberi pakan tepung Ulva sp. (146,07 ± 1,73 µm/hari).The most common problem in abalone seed production is the high mortality occurrence (> 90%) after postlarvae settlement to the rearing plates. The use of microparticle diets to replace the natural feed of postlarval has been performed on various species of fish, shrimp, and abalone. This research aims to determine the most effective and suitable powder-based feed to support the survival and growth of abalone Haliotis squamata larvae. The experiments consisted of five feed treatments, i.e., Spirulina sp., Ulva sp., Chaetoceros sp., and Gracilaria sp. Flour, and diatoms (as control). Each treatment had four replicates. The powder-based feed used in each treatment was firstly mixed with a solution of agar powder (7.5 mg/mL sea water, 40°C) with a concentration of 40 mg of flour/mL of agar solution. Feeding was done every three days by spraying the feed solution onto the surface of the larval rearing plate. The study was conducted for 30 days. The results showed that survival rate of abalone larvae fed with Spirulina sp. flour was the highest and significantly different (P<0.05) compared with those given diatoms, Chaetoceros sp. and Ulva sp. flours, which were 81.49%, 79.25%, 76.57%, and 76.46%, respectively, and not significantly different from those fed with Gracilaria sp. 81.37% (P>0.05). The highest daily growth rate of the shell length of abalone larvae was achieved by larvae fed with Gracilaria sp. (203.81 ± 1.23 ¼m/day) and Spirulina sp. flours (205.47 ± 1.71 µm/day). The lowest daily growth rate of shell length was found on abalone larvae fed with Ulva sp. flour (146.07 ± 1.73 µm/day).


2009 ◽  
Vol 8 (2) ◽  
pp. 199 ◽  
Author(s):  
S. Sarah ◽  
. Widanarni ◽  
Agus Oman Sudrajat

<p>This experiment was conducted to study the stocking density effect on growth and survival rate of giant gouramy seed in the aquarium. Fish were stocked at the density of 2.5; 5.0; 7.5 and 10 individual/l with average initial weight and length were 0.013 g and 5.56 mm, respectively. Fish were fed with silkworm (<em>Tubifex</em> sp.) <em>ad libitum</em> in the morning and evening for 22 days of experiment. Aquarium was siphoned and water was changed everyday for maintaining good water quality. Result showed that survival rate remain high in all treatment (93.5 - 95.5%). Increasing stocking density from 2.5 to 10 individual/I did not affect survival rate. On the other hand growth rate and feed efficiency decreased, while fish yield increased. Stocking density of 2,5 individual/l gave  the highest daily growth rate, individual growth rate, growth in length and feed efficiency of 12.94%, 0.0081 g/day, 16.84 mm and 12.51%; respectively. The highest value for fish yield (0,0360 g/l/day) was obtained from stocking density of 10 individual/l</p> <p>Key words: stocking density, seed, survival rate, growth rate, giant gouramy <em>Osphrenemus goramy</em></p> <p> </p> <p>ABSTRAK</p> <p>Keterbatasan pengadaan benih ikan gurame (<em>Osphronemus gourame </em>Lac.) disebabkan oleh tidak seimbangnya jumlah benih yang tersedia dengan kebutuhan usaha pembesaran. Teknik pembenihan yang digunakan sampai saat ini relatif sederhana dengan cara tradisional. Pemeliharaan gurame secara terkontrol di akuarium dapat menjawab tantangan dalam teknologi pembenihan gurame sekaligus sebagai sarana pola budidaya secara bertahap yang sedang berkembang saat ini. Produksi yang tinggi akan dicapai dengan pemeliharaan pada kepadatan yang tinggi. Pada keadaan lingkungan yang baik dan pakan yang mencukupi, peningkatan kepadatan akan disertai dengan peningkatan hasil. Jumlah ikan yang ditebar pada penelitian ini disesuaikan berdasarkan perlakuan yaitu 2,5; 5,0; 7,5 dan 10 ekor/l. Pengamatan terhadap ikan dilakukan sampai hari ke-22. Hasil pengamatan menunjukkan bahwa peningkatan kepadatan 2,5 - 10 ekor/l pada pemeliharaan benih gurame di akuarium mempengaruhi pertumbuhan, hasil dan efisiensi pakan, namun tidak berpengaruh terhadap kelangsungan hidup. Dengan meningkatnya padat penebaran, laju pertumbuhan dan efisiensi pakan semakin menurun, sedangkan hasil (<em>yield</em>) semakin meningkat. Padat penebaran 2,5 ekor/l menghasilkan laju pertumbuhan harian, laju pertumbuhan individu, pertumbuhan panjang mutlak dan efisiensi pakan tertinggi, masing-masing mencapai 12,94 %, 0,0081 g/hari, 16,84 mm dan 12,51 %. Sedangkan nilai tertinggi untuk hasil (<em>yield</em>) sebesar 0,0360 g/l/hari diperoleh pada padat penebaran 10,0 ekor/l.</p> <p>Kata kunci : padat tebar, pembenihan, gurame dan <em>Osphronemus gouramy</em></p>


Author(s):  
Ayi Yustiati ◽  
Mahmud Sa’id ◽  
. Junianto ◽  
Ibnu Bangkit Bioshina Suryadi

This research aimed to determine the optimal dosage of potassium diformate addition to commercial feed in order to increase the growth and survival rate of common carp seeds. The method used in this research was experimental in a completely randomized design (CRD) consisting of five treatments and four replications. The treatments used were A (control), B with addition of potassium diformate (0.2%), C (0.3%), D (0.4%), and E (0.5%). For test fish, there were 200 of Common carp seeds with a size of 2-3 cm. The media used was 20 aquariums with a size of 40x30x40 cm3 and a stocking density of 10 individuals/aquarium. The rearing period was 35 days and the amount of feed given was 3% of the fish body weight. The parameters observed included water quality (temperature, pH, and dissolved oxygen) which were observed every 7 days for 35 days. Other parameters  monitored were daily growth rate, survival rate, feed efficiency, and the acidity level of the intestine. The results showed that 0.3% addition of potassium diformate gave the best results with a daily growth rate value of 3.21%, highest survival of 98%, efficiency of feed utilization of 64.83%, and a decrease in intestinal pH which helped the process of protein absorption in the fish body. In conclution, the addition of potassium diformate has good effect on growth and survival rates of common carp.


Author(s):  
Ayi Yustiati ◽  
Esa Jaya Dinata ◽  
Herman Hamdani ◽  
Ibnu Bangkit

This research was conducted to determine the optimal density of gorami (Osphronemus goramy Lac 1801) and nilem (Osteochilus hasselti C.V) polyculture with varios nilem density. The research are carried out in the maintenance pool of the Kawungsari Fish Farmers Group, Kertayasa Village, Pangandaran Regency, Indonesia. The research method used is an experimental method with 4 treatments and 4 replications using Completely Randomized Design (CRD). Variation of stocking densities carried out with the ratio of gorami: nilem is 30 : 0 fish m2 -1 (control), 30:10 fish m2 -1, 30: 20 fish m2 -1 and 30: 30 fish m2 -1. The fish are kept for 40 days in a pond with an area of 6 x 8 m and a height of 1.2 m. The fish used are gorami with 6-8 cm long and nilem with 4-6 cm long, gorami fish from the PangandaranKertayasa farmer group and nilem fish come from the seed hall which triggers gorami stock and nilem (BPPSIGN) Tasikmalaya. Obtained data are done at the beginning of the study and every 10 days, namely on day 10th, 20th, 30th and 40th. The parameters observed included daily growth rate, survival rate, feed efficiency, periphyton abundance and water quality. The results showed the stocking density of 30 fish m2 -1gorami with 20 fish m2 -1nilem was the most optimal stocking density with survival rate of 93.5%, daily growth rate of 1.19%, and gorami’s feed efficiency is 32.66%. Pond water quality including temperature, pH and dissolved oxygen is still within optimum range in accordance with the Indonesian National Standard for maintenance of gorami and nilem.


2020 ◽  
Vol 20 (2) ◽  
pp. 221-230
Author(s):  
Nguyen Xuan Thanh ◽  
Le Duc Cuong ◽  
Le Minh Hiep ◽  
Dao Thi Anh Tuyet

This study examined the effect of stocking density on growth and survival of tilapia cultured in biofloc technology system. Three different stocking densities cultured in biofloc technology were 6 fish/m3, 8 fish/m3 and 10 fish/m3 for 86 days in triplicate for each treatment. The stocking density of the control lot was 3 fish/m3 cultured without biofloc technology. Initial stocking weight ranged from 2–3 g/fish. The water quality parameters were monitored and regulated in the suitable ranges for biofloc technology and for the growth and development of tilapia. The results showed that specific growth rate of fish cultured at a density of 6 fish/m3 was higher than that in the treatments of 8 fish/m3 and 10 fish/m3 with the average values of 5.72%; 5.62% and 5.43%, respectively, and the specific growth rate of fish in the control treatment was 5.71%. Daily growth rate of fish cultured at a density of 6 fish/m3 was higher than that cultured at densities of 8 fish/m3 and 10 fish/m3 with average values of 3.19 g/day, 2.98 g/day, and 2.55 g/day, respectively; and the daily growth rate of the control treatment was 3.27 g/day. Survival rate of tilapia cultured at densities of 6 fish/m3 and 8 fish/m3 was 100%, whereas survival rate of tilapia cultured at a density of 10 fish/m3 was 95.75%, and it was 88.9% for the control lot. The research results provide a scientific basis to propose tilapia culture technique in biofloc technology in brackish water, with the density of 6–8 fish/m3.


AQUASAINS ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 903
Author(s):  
Pratiwi Pratiwi ◽  
Muhammad Marzuki ◽  
Bagus Dwi Hari Setyono

Litopenaeus vannamei is a commercial shrimp that has high economic value. In recent years, the main commodity contributing to the aquaculture sector in Indonesia is vaname shrimp. Vaname shrimp cultivation aims to meet the increasing market demand. One of the factors that influence the success of vaname shrimp cultivation is the stocking density. This study aims to determine the growth and viability of PL-10 vaname shrimp (Litopenaeus vannamei) fry at different stocking densities. This study used a completely randomized design (CRD). There were 4 treatments, namely P1 with a stocking density of 75 heads / 40 liters, P2 with a stocking density of 150 heads / 40 liters, P3 with a stocking density of 225 heads / 40 liters, and P4 with a stocking density of 300 heads / 40 liters, respectively. 4 repetitions. The research data were analyzed using statistical analysis Univariate One Way Analysis of Variance at 5% level with a 95% confidence interval. The results showed that the highest daily growth rate was obtained in treatment P1 with an average value of 26.836 ± 0.745% / day, the highest daily length growth rate was obtained in treatment P1 with an average value of 6.719 ± 0.103% / day, the highest absolute weight growth was obtained at treatment P1. P1 treatment with an average value of 0.031 ± 0.027 gram, absolute length growth was obtained in treatment P1 with an average value of 23.025 ± 0.667 mm, the highest survival rate was obtained in treatment P2 with an average value of 96.333 ± 3.549%, but not different real with treatment P2 (93.333 ± 3.443%) and treatment P4 (92.417 ± 2.062). The conclusion of this study is that stocking density has a significant effect on the growth of vannamei shrimp which are being maintained at the PL-10 stage. The best stocking density in this study was P1 treatment (75 fish / 40 liters).


2016 ◽  
Vol 8 (3) ◽  
pp. 26
Author(s):  
Ma Hoseop ◽  
Ettagbor Hans Enukwa ◽  
Kang Wonseok ◽  
Kim Choonsig

The purpose of this research was to investigate the use of drilled slanted holes filled with different substrates in the revegetation of rock slopes. Slanted holes were drilled on rock slopes using pneumatic drillers in an abandoned limestone quarry area. These holes were filled with twelve different substrates, treated with Trichoderma harzianum. One climbing plant of Ivy (Hedera rhombea), a native woody plant (Lespedeza cyrtobotrya), three native herbaceous plants (Dianthus barbatus, Siberian chrysanthemum and Aster koraiensis) and one exotic grass plant (Festuca arundinacea) were planted in each of the soils. Six months after planting, only Ivy, Festuca arundinacea, Lespedeza cyrtobotrya and Dianthus barbatus were still observed growing on the soils, while the other plant species had already died in all the 12 soil treatments. Leaf mold soil + 50% Trichoderma harzianum provide the best conditions for revegetation of Festuca arundinacea, as this plant had its highest mean height, high survival rate, with impressive growth rate on this soil. Lespedeza cyrtobotrya also had its most favorable growth on Leaf mold soil + 50% T. harzianum. Mixed soil + 100% T. harzianum provide the best conditions for revegetation of Ivy. Leaf mold soil + 100% T. harzianum provide the best condition for revegetation of Dianthus barbatus as it had its highest mean height, highest growth rate and high survival rate on this soil. Siberian chrysanthemum and Aster koraiensis had the worse results as all of the plants died within a short period of time after planting. Ivy had the highest mean height, followed by Festuca arundinacea. The planted Ivy and Festuca arundinacea now form the vegetation of the research site which together with the substrate is now providing habitat to some wildlife like some species of centipedes, earthworms, insects and wild plants. Less than one year after planting, F. arundinacea already produced flowers and seeds which would further increase the plant population after dispersal and germination. Drilling slanted holes on rock slopes is therefore a feasible method to be used for revegetation and restoration of degraded quarries.The revegetation of steep rock slopes of quarries is a very challenging activity. However, the method of drilling slanted holes on rock slopes is a feasible method to be used for restoration of degraded quarries. These holes could serve in soil deposition and water retention that will further enhance plant growth.Methods of improving seed germination as well as enhancing growth of plants are also imperative in revegetation success of such degraded landscapes. Different percentage mixtures of T. harzianum in different soils could enhance plant growth depending on the target plant species. Different substrates could thus be used in such revegetation process depending on the target plant species.Revegetation would provide habitat and improve landscape beauty and stability, thus it is important for successful restoration of rock slopes 


2017 ◽  
Vol 8 (2) ◽  
pp. 747
Author(s):  
Fazril Saputra ◽  
Dinamella Wahjuningrum ◽  
Kustiariyah Tarman ◽  
Irzal Effendi

<em>This study aimed to determine the best dose of administration of marine fungal <span style="text-decoration: underline;">Nodulisporium</span> sp. KT29 metabolites to improve the production performance of marine culture of white shrimp. The experimental animals used were white shrimp <span style="text-decoration: underline;">Litopenaeus</span> <span style="text-decoration: underline;">vannamei</span> post larvae 12, which were reared in the sea using floating net cages with a stocking density of 700 post larvae/net. Experimental design used was a completely randomized design consisting of 3 treatments with 3 replications; control without the administration of <span style="text-decoration: underline;">Nodulisporium</span> sp. KT29 metabolites, dietary of feed containing <span style="text-decoration: underline;">Nodulisporium</span> sp. KT29 metabolites at doses of 20 mL/kg of feed and 40 mL/kg of feed. The results showed that survival rate, absolute length growth, weight gain, daily growth rate and feed conversion ratio in treatment groups were better than the control (P&lt;0.05). The administration of marine fungal <span style="text-decoration: underline;">Nodulisporium</span> sp. KT29 metabolites at a dose of 20 mL/kg of feed could improve the production performance of white shrimp cultivated in the sea with survival rate, daily growth rate and feed conversion ratio: 66.61±6.94%, 20.18±0.39 %/day and 3.20±0.22, respectively.</em>


2018 ◽  
Vol 47 ◽  
pp. 02007
Author(s):  
Lies Setijaningsih ◽  
Dewi Puspaningsih ◽  
Imam Taufik

Fulfill needs of Siamese gourami still rely on the catch from nature, so the population in nature has become decreased. The increase of Siamese gourami fish production from culture is still constrained by the survival rate and slow growth rate in the seed phase. The purpose of this research was to determine the effect of Siamese gourami stocking density on the application of optimum temperature and salinity to growth performance for optimization of production capacity. Application of optimum temperature and salinity refers to the results of previous research, i.e., temperature of 28 °C and salinity 3 ‰. Siamese gourami seed with length of 2.96 ± 0.42 cm and weight of 5.27 ± 0.10 g used in the research. The experimental design used completely randomized design with four treatments, i.e. 1 fish L-1, 2 fish L-1, 3 fish L-1 and 4 fish L-1. The results showed that there were an effect of different stocking density on absolute growth weight and length, survival rate, specific growth rate and productivity. This is shown in the C treatment which growth was 4.95 ± 0.037 g, length 3.51 ± 0.01 cm, survival rate 93.47%, specific growth rate 1.22% and fish productivity of 625.76 g.


2016 ◽  
Vol 5 (2) ◽  
pp. 98-106
Author(s):  
MA Hossain ◽  
MJ Islam ◽  
MM Uddin ◽  
MM Hossain ◽  
M Kunda

This study was conducted to investigate and compare the growth performance of four Tilapia strains in hapa nursing with the aim to find out the best strain of tilapia. The research was conducted by four treatments with five replicates each. The tilapia fry were collected from, BFRI source from BFRI HQ, Mymensingh stocked in Treatment-1; unknown source from Hi-Tech Tilapia Hatchery Dagoanbhuiya, Feni in Treatment-2, Philipine strain from Green Field Tilapia Hatchery, Laxmipur in Treatment-3 and fry from Breeding Cohort, Sonar Bangla Hatchery, Bamna, Barguna in Treatment-4. The Individual stocking weight (g) of fry was average 0.01g. The fries were stocked in 12 hapa at same stocking density of 1200 fish/m2. Each of the hapa was 2m × 1m × 1m size and fixed in an earthen ponds. Supplementary feed with 31.29% of protein level consisting of rice bran (25%), mustard oil cake (15%), wheat flour (30%) and fish meal (30%) was given at the rate of 40% of their body weight per day manually, four times a day at the beginning of the experimental period and reduced to 25% of their body weight and continued up to harvesting period. The results showed that fry of Treatment-4 strain was the best strain and had significantly (p < 0.05) higher final mean body weight (FMBW), mean body weight gain (MBWG), specific growth rate (SGR) (9.75%), gross yield (GY) (2.13) and Net yield (NY) (2.12) than the Treatment-1 , Treatment-2 strains and Treatemnt-3 strain. Treatment-4 strain had the fastest growth rate, whereas the lowest performing strain was that from Treatment-2. We also analyzed survival rate of the fish from stocking to harvest, survival rate was highest in Treatment-4 strain (80%) where lowest is Treatment-3. Treatment-4 stain also showed the best feed conversion ratio (FCR) 1.62 than others. Considering the overall performance it can be concluded that tilapia strain collected from Breeding Cohort source is best among the four strains. However, further research may still be needed to compare more strains from different water body to evaluate the genetic potential for Tilapia.International Journal of Natural Sciences (2015), 5(2) 98-106


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