Hydroxyapatite-Bond Coat Systems for Improved Mechanical and Biological Performance of HIP Implants

1998 ◽  
Author(s):  
R.B. Heimann ◽  
H. Kurzweg ◽  
T.A. Vu
Keyword(s):  
Adhesion Strength ◽  
Bond Coat ◽  
Body Fluid ◽  
Peel Test ◽  
Ceramic Materials ◽  
Coating System ◽  
Bond Coats ◽  
Biological Performance ◽  
Bioinert Ceramic

Abstract Bond coats based on bioinert ceramic materials such as titania and zirconia were developed to increase the adhesion strength of the coating system hydroxyapatite/bond coat to Ti6A14V alloy surfaces used for hip endoprostheses and dental root implants. The bond coats improved the adhesion strength, measured by a modified ASTM D3167-76 peel test, by 50 to 100% and also the resorption resistance as determined by in vitro leaching in simulated body fluid (Hank's Balanced Salt Solution, HBSS) for up to 28 days.

Study of Phosphocalcic Glasses from SiO2-CaO-P2O5 System with and Without Silver II. The bioactivity analysis by FTIR, SEM methods and microbiological study of silver-doped glasses

2017 ◽  
Vol 68 (6) ◽  
pp. 1188-1192
Author(s):  
Daniela Avram ◽  
Nicolae Angelescu ◽  
Dan Nicolae Ungureanu ◽  
Ionica Ionita ◽  
Iulian Bancuta ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Body Fluid ◽  
Pathogenic Bacteria ◽  
De Novo ◽  
Microbiological Examination ◽  
Doped Glasses ◽  
P2o5 System ◽  
Scanning Electron

The study in vitro of the glass powders bioactivity was performed by soaking them in simulated body fluid for 3 to 21 days at a temperature of 37�C and pH = 7.20. The synthesis de novo of hydroxyapatite, post soaking was confirmed by Fourier Transform Infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). The study of the antimicrobial activity was performed by microbiological examination on two strains of pathogenic bacteria involved in postoperative nosocomial infections.

IN-VITRO BEHAVIOUR OF BIPHASIC CALCIUM PHOSPHATE WITH DIFFERENT RATIO OF SILICA CONTENT IN SIMULATED BODY FLUID

2015 ◽  
Vol 23 (1) ◽  
pp. 1-14
Author(s):  
Sudirman Sahid ◽  
◽  
Nor Shahida Kader Bashah ◽  
Salina Sabudin ◽  
◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Simulated Body Fluid ◽  
Body Fluid ◽  
Biphasic Calcium Phosphate ◽  
Silica Content

scholarly journals Tetracalcium Phosphate/Monetite/Calcium Sulfate Hemihdrate Biocement Powder Mixtures Prepared by the One-Step Synthesis for Preparation of Nanocrystalline Hydroxyapatite Biocement-Properties and In Vitro Evaluation

Materials ◽  
2021 ◽  
Vol 14 (9) ◽  
pp. 2137
Author(s):  
Lubomir Medvecky ◽  
Maria Giretova ◽  
Radoslava Stulajterova ◽  
Lenka Luptakova ◽  
Tibor Sopcak
Keyword(s):  
Simulated Body Fluid ◽  
Body Fluid ◽  
Calcium Sulfate ◽  
Powder Mixtures ◽  
Liquid Component ◽  
Tetracalcium Phosphate ◽  
Nanocrystalline Hydroxyapatite ◽  
One Step ◽  
Calcium Sulfate Hemihydrate

A modified one-step process was used to prepare tetracalcium phosphate/monetite/calcium sulfate hemihydrate powder cement mixtures (CAS). The procedure allowed the formation of monetite and calcium sulfate hemihydrate (CSH) in the form of nanoparticles. It was hypothesized that the presence of nanoCSH in small amounts enhances the in vitro bioactivity of CAS cement in relation to osteogenic gene markers in mesenchymal stem cells (MSCs). The CAS powder mixtures with 15 and 5 wt.% CSH were prepared by milling powder tetracalcium phosphate in an ethanolic solution of both orthophosphoric and sulfuric acids. The CAS cements had short setting times (around 5 min). The fast setting of the cement samples after the addition of the liquid component (water solution of NaH2PO4) was due to the partial formation of calcium sulfate dihydrate and hydroxyapatite before soaking in SBF with a small change in the original phase composition in cement powder samples after milling. Nanocrystalline hydroxyapatite biocement was produced by soaking of cement samples after setting in simulated body fluid (SBF). The fast release of calcium ions from CAS5 cement, as well as a small rise in the pH of SBF during soaking, were demonstrated. After soaking in SBF for 7 days, the final product of the cement transformation was nanocrystalline hydroxyapatite. The compressive strength of the cement samples (up to 30 MPa) after soaking in simulated body fluid (SBF) was comparable to that of bone. Real time polymerase chain reaction (RT-PCR) analysis revealed statistically significant higher gene expressions of alkaline phosphatase (ALP), osteonectin (ON) and osteopontin (OP) in cells cultured for 14 days in CAS5 extract compared to CSH-free cement. The addition of a small amount of nanoCSH (5 wt.%) to the tetracalcium phosphate (TTCP)/monetite cement mixture significantly promoted the over expression of osteogenic markers in MSCs. The prepared CAS powder mixture with its enhanced bioactivity can be used for bone defect treatment and has good potential for bone healing.

A study on using expanded perlite with hydroxyapatite: Reinforced bio-composites

2021 ◽  
pp. 095441192199656
Author(s):  
Erdoğan Karip ◽  
Mehtap Muratoğlu
Keyword(s):  
Body Fluid ◽  
Xrd Analysis ◽  
Cold Isostatic Pressing ◽  
Expanded Perlite ◽  
X Ray Diffraction ◽  
Pressing Method ◽  
X Ray ◽  
Infra Red ◽  
Ft Ir

People are exposed to different kinds of diseases or various accidents in life. Hydroxyapatite (HA) has been widely employed for bone treatment applications. In this study, HA was extracted from sheep bones. Bio-composites were doped with 1, 5, and 10 wt.% of expanded perlite and 5 wt.% of ZrO2–MgO-P2O5. The bio-composites were prepared by the cold isostatic pressing method (250 MPa) and sintered at 900°C for 1 h. In order to evaluate the characteristics of the bio-composites, microhardness, density, X-ray diffraction (XRD), Fourier transform infra-red spectroscopy (FT-IR), scanning electron microscopy (SEM), and energy dispersive spectroscopy (EDS) analyses were carried out on them. Additionally, the specimens whose characteristics were determined were kept in synthetic body fluid (SBF), and their in vitro behavior was examined. As a result, it was observed that microhardness increased as both the weight and the grain size of the expanded perlite were increased. Calcium silicate, tri-calcium phosphate, and hydroxyapatite were observed in the XRD analysis of all samples, and the formation of apatite structures was increased by addition of ZrO2–MgO–P2O5.

scholarly journals Preparation and Degradation Characteristics of MAO/APS Composite Bio-Coating in Simulated Body Fluid

Coatings ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 667
Author(s):  
Zexin Wang ◽  
Fei Ye ◽  
Liangyu Chen ◽  
Weigang Lv ◽  
Zhengyi Zhang ◽  
...  
Keyword(s):  
Simulated Body Fluid ◽  
Composite Coating ◽  
Body Fluid ◽  
Composite Coatings ◽  
Degradation Process ◽  
Ceramic Coatings ◽  
Immersion Test ◽  
Substrate Material ◽  
Atmospheric Plasma

In this work, ZK60 magnesium alloy was employed as a substrate material to produce ceramic coatings, containing Ca and P, by micro-arc oxidation (MAO). Atmospheric plasma spraying (APS) was used to prepare the hydroxyapatite layer (HA) on the MAO coating to obtain a composite coating for better biological activity. The coatings were examined by various means including an X-ray diffractometer, a scanning electron microscope and an energy spectrometer. Meanwhile, an electrochemical examination, immersion test and tensile test were used to evaluate the in vitro performance of the composite coatings. The results showed that the composite coating has a better corrosion resistance. In addition, this work proposed a degradation model of the composite coating in the simulated body fluid immersion test. This model explains the degradation process of the MAO/APS coating in SBF.

scholarly journals The in vitro assessment of the toxicity of volatile, oxidisable, redox-cycling compounds: phenols as an example

2021 ◽  
Author(s):  
Laia Tolosa ◽  
Teresa Martínez-Sena ◽  
Johannes P. Schimming ◽  
Erika Moro ◽  
Sylvia E. Escher ◽  
...  
Keyword(s):  
Cultured Cells ◽  
Degradation Products ◽  
Degradation Kinetics ◽  
Redox Cycling ◽  
In Vitro Toxicity ◽  
Cross Contamination ◽  
Physicochemical Changes ◽  
In Vitro Assessment ◽  
Biological Performance

AbstractPhenols are regarded as highly toxic chemicals. Their effects are difficult to study in in vitro systems because of their ambiguous fate (degradation, auto-oxidation and volatility). In the course of in vitro studies of a series of redox-cycling phenols, we found evidences of cross-contamination in several in vitro high-throughput test systems, in particular by trimethylbenzene-1, 4-diol/trimethylhydroquinone (TMHQ) and 2,6-di-tertbutyl-4-ethylphenol (DTBEP), and investigated in detail the physicochemical basis for such phenomenon and how to prevent it. TMHQ has fast degradation kinetics followed by significant diffusion rates of the resulting quinone to adjacent wells, other degradation products being able to air-diffuse as well. DTBEP showed lower degradation kinetics, but a higher diffusion rate. In both cases the in vitro toxicity was underestimated because of a decrease in concentration, in addition to cross-contamination to neighbouring wells. We identified four degradation products for TMHQ and five for DTBEP indicating that the current effects measured on cells are not only attributable to the parent phenolic compound. To overcome these drawbacks, we investigated in detail the physicochemical changes occurring in the course of the incubation and made use of gas-permeable and non-permeable plastic seals to prevent it. Diffusion was greatly prevented by the use of both plastic seals, as revealed by GC–MS analysis. Gas non-permeable plastic seals, reduced to a minimum compounds diffusion as well oxidation and did not affect the biological performance of cultured cells. Hence, no toxicological cross-contamination was observed in neighbouring wells, thus allowing a more reliable in vitro assessment of phenol-induced toxicity.

scholarly journals Influence of Different Annealing Atmospheres on the Mechanical Properties of Freestanding MCrAlY Bond Coats Investigated by Micro-Tensile Creep Tests

Metals ◽  
2019 ◽  
Vol 9 (6) ◽  
pp. 692 ◽  
Author(s):  
Sven Giese ◽  
Steffen Neumeier ◽  
Jan Bergholz ◽  
Dmitry Naumenko ◽  
Willem J. Quadakkers ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Creep Strength ◽  
Bond Coat ◽  
Oxide Dispersion Strengthened ◽  
Tensile Creep ◽  
Creep Tests ◽  
Bond Coats ◽  
Nial Phase ◽  
Lower Strain ◽  
Plasma Sprayed

The mechanical properties of low-pressure plasma sprayed (LPPS) MCrAlY (M = Ni, Co) bond coats, Amdry 386, Amdry 9954 and oxide dispersion strengthened (ODS) Amdry 9954 (named Amdry 9954 + ODS) were investigated after annealing in three atmospheres: Ar–O2, Ar–H2O, and Ar–H2–H2O. Freestanding bond coats were investigated to avoid any influence from the substrate. Miniaturized cylindrical tensile specimens were produced by a special grinding process and then tested in a thermomechanical analyzer (TMA) within a temperature range of 900–950 °C. Grain size and phase fraction of all bond coats were investigated by EBSD before testing and no difference in microstructure was revealed due to annealing in various atmospheres. The influence of annealing in different atmospheres on the creep strength was not very pronounced for the Co-based bond coats Amdry 9954 and Amdry 9954 + ODS in the tested conditions. The ODS bond coats revealed significantly higher creep strength but a lower strain to failure than the ODS-free Amdry 9954. The Ni-based bond coat Amdry 386 showed higher creep strength than Amdry 9954 due to the higher fraction of the β-NiAl phase. Additionally, its creep properties at 900 °C were much more affected by annealing in different atmospheres. The bond coat Amdry 386 annealed in an Ar–H2O atmosphere showed a significantly lower creep rate than the bond coat annealed in Ar–O2 and Ar–H2–H2O atmospheres.

THE STUDY ON GRANTING BIOACTIVITY OF Ti ALLOY BY SURFACE TREATMENT

2010 ◽  
Vol 17 (02) ◽  
pp. 153-157 ◽  
Author(s):  
N. R. HA ◽  
Z. X. YANG ◽  
G. C. KIM ◽  
K. H. HWANG ◽  
D. S. SEO ◽  
...  
Keyword(s):  
Surface Treatment ◽  
Body Fluid ◽  
Surface Effect ◽  
In Vitro Test ◽  
Ti Alloy ◽  
X Ray Diffraction ◽  
X Ray ◽  
Chemical Surface ◽  
Vitro Test

Titanium alloys are superior of biocompatibility, mechanical properties and chemical stability. The biocompatibility of Ti alloy is related to the surface effect between human tissue and implant. Therefore, the purpose of this study is to investigate the bioactivity of Ti alloy by alkali and acid chemical surface treatment; and the biocompatibility of Ti alloy was evaluated by in vitro test. Higher bone-bonding ability and bioactivity of the substrate were obtained by the formation of apatite layers on the Ti alloy in simulated body fluid. The microstructures of apatite layer were investigated by scanning electron microscope (SEM) and the formed phases were analyzed with X-ray diffraction (XRD).

scholarly journals The Sertoli-Spermatid Junctional Complex Adhesion Strength Is Affected In Vitro by Adjudin

2006 ◽  
Vol 27 (6) ◽  
pp. 790-794 ◽  
Author(s):  
K. M. Wolski ◽  
D. D. Mruk ◽  
D. F. Cameron
Keyword(s):  
Adhesion Strength ◽  
Junctional Complex
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