BsmI AND HinfI POLYMORPHISMS OF THE FABP4 GENE OF DIFFERENT PIG BREEDS OF THE BELGOROD REGION IN RUSSIA

Leptin receptor is one of the components of the system of regulating energy homeostasis of the organism. Leptin receptor gene (LEPR) polymorphism is associated with pig carcass index of the content of intramus- cular fat in its valuable parts, which is particularly important when assessing the quality of their carcasses for processing. Intramuscular fat is associated with meat fl avor characteristics and partly determines its tenderness, juiciness, and other parameters. Aim. To analyze LEPR gene (SNP NM001024587.1, p. 1987 C > T) polymor- phism in populations of various pig breeds and to establish its relationship with the quality of both meat and fat of pigs of Large White breed of Ukrainian breeding. Methods. Genetic-population analysis of nine pig breeds, associative analysis on the search connection of LEPR gene polymorphism with quality of both meat and fat of pigs of Large White breed of Ukrainian breeding. LEPR locus genotyping was performed by High Resolution Melting (HRM). Results. All the studied breeds are characterized by polymorphism of the leptin receptor gene (SNP NM001024587.1, p. 1987 C > T), signifi cant breed specifi city in the distribution of frequencies of alleles was established. Statistically confi rmed effect (p < 0.05) of genotypes LEPR on the content of intramuscular fat, total dry matter and moisture in the meat, as well as the moisture content in the back fat of pigs of Ukrainian Large White breed was revealed. Higher content of intramuscular fat was found in the animals with genotype TT, while a smaller amount of intramuscular fat and more moisture in fat was revealed in heterozygotes. Conclusions. Genetic marker LEPR SNP NM001024587.1, p. 1987 C > T can be used in the marker-assisted selection to predict and improve the performance quality of the meat of pigs of Large White breed of the Ukrainian breeding. These results suggest that porcine leptin receptor gene controls the quality of fat comp- lex – inside muscles and in the dorsal part of the carcass.

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Integrated analysis of non-coding RNA and mRNA expression profiles of 2 pig breeds differing in muscle traits

Journal of Animal Science ◽

10.2527/jas2016.0867 ◽

2017 ◽

Vol 95 (3) ◽

pp. 1092 ◽

Cited By ~ 4

Author(s):

J. Sun ◽

M. Xie ◽

Z. Huang ◽

H. Li ◽

T. chen ◽

...

Keyword(s):

Mrna Expression ◽

Expression Profiles ◽

Integrated Analysis ◽

Pig Breeds ◽

Non Coding Rna

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Transcriptomic Profiling of Skeletal Muscle Reveals Candidate Genes Influencing Muscle Growth and Associated Lipid Composition in Portuguese Local Pig Breeds

Animals ◽

10.3390/ani11051423 ◽

2021 ◽

Vol 11 (5) ◽

pp. 1423

Author(s):

André Albuquerque ◽

Cristina Óvilo ◽

Yolanda Núñez ◽

Rita Benítez ◽

Adrián López-Garcia ◽

...

Keyword(s):

Candidate Genes ◽

Muscle Growth ◽

Rna Seq ◽

Next Generation Sequencing Technology ◽

Pig Breeds ◽

Nfkb Activation ◽

Main Gene ◽

Slow Type ◽

Longissimus Lumborum ◽

Generation Sequencing

Gene expression is one of the main factors to influence meat quality by modulating fatty acid metabolism, composition, and deposition rates in muscle tissue. This study aimed to explore the transcriptomics of the Longissimus lumborum muscle in two local pig breeds with distinct genetic background using next-generation sequencing technology and Real-Time qPCR. RNA-seq yielded 49 differentially expressed genes between breeds, 34 overexpressed in the Alentejano (AL) and 15 in the Bísaro (BI) breed. Specific slow type myosin heavy chain components were associated with AL (MYH7) and BI (MYH3) pigs, while an overexpression of MAP3K14 in AL may be associated with their lower loin proportion, induced insulin resistance, and increased inflammatory response via NFkB activation. Overexpression of RUFY1 in AL pigs may explain the higher intramuscular (IMF) content via higher GLUT4 recruitment and consequently higher glucose uptake that can be stored as fat. Several candidate genes for lipid metabolism, excluded in the RNA-seq analysis due to low counts, such as ACLY, ADIPOQ, ELOVL6, LEP and ME1 were identified by qPCR as main gene factors defining the processes that influence meat composition and quality. These results agree with the fatter profile of the AL pig breed and adiponectin resistance can be postulated as responsible for the overexpression of MAP3K14′s coding product NIK, failing to restore insulin sensitivity.

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Function of the Porcine TRPC1 Gene in Myogenesis and Muscle Growth

Cells ◽

10.3390/cells10010147 ◽

2021 ◽

Vol 10 (1) ◽

pp. 147

Author(s):

Yu Fu ◽

Peng Shang ◽

Bo Zhang ◽

Xiaolong Tian ◽

Ruixue Nie ◽

...

Keyword(s):

Transient Receptor Potential ◽

Growth Traits ◽

Expression Profiles ◽

Muscle Growth ◽

Receptor Potential ◽

Transient Receptor Potential Channel ◽

Nucleotide Polymorphisms ◽

Pig Breeds ◽

Muscle Tissues ◽

Transient Receptor

In animals, muscle growth is a quantitative trait controlled by multiple genes. Previously, we showed that the transient receptor potential channel 1 (TRPC1) gene was differentially expressed in muscle tissues between pig breeds with divergent growth traits base on RNA-seq. Here, we characterized TRPC1 expression profiles in different tissues and pig breeds and showed that TRPC1 was highly expressed in the muscle. We found two single nucleotide polymorphisms (SNPs) (C-1763T and C-1604T) in TRPC1 that could affect the promoter region activity and regulate pig growth rate. Functionally, we used RNAi and overexpression to illustrate that TRPC1 promotes myoblast proliferation, migration, differentiation, fusion, and muscle hypertrophy while inhibiting muscle degradation. These processes may be mediated by the activation of Wnt signaling pathways. Altogether, our results revealed that TRPC1 might promote muscle growth and development and plays a key role in Wnt-mediated myogenesis.

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