Bidirectional Selection for Nitrogenase Activity and Shoot Dry Weight Among Late Generation Progenies of a Virginia x Spanish Peanut Cross1

1986 ◽  
Vol 13 (2) ◽  
pp. 86-89 ◽  
Author(s):  
S. Arrendell ◽  
J. C. Wynne ◽  
G. H. Elkan ◽  
T. J. Schneeweis
Keyword(s):  
Nitrogen Fixation ◽  
Plant Growth ◽  
Growth Habit ◽  
Nitrogenase Activity ◽  
Dry Weight ◽  
Fruit Weight ◽  
Shoot Dry Weight ◽  
Plant Growth Habit ◽  
The Mean ◽  
Selection For

Abstract Improvement of the host contribution to nitrogen fixation has been proposed as a method of increasing nitrogen fixation. Significant variability and generally high broad-sense heritability estimates (.60 ± .27 to .82 ± .26 for nitrogenase activity and .53 ± .29 to .85 ± .26 for shoot dry weight) have been reported for F2-derived families from a cross between the Virginia (Arachis hypogaea L. ssp. hypogaea var. hypogaea) cultivar NC 6 and the Spanish (ssp. fastigiata Waldron vulgaris Harz.) breeding line 922, indicating selection for increased nigtogen fixation should be effective in this population. Lines from this population were chosen randomly from F2-derived families selected for high and low nitrogenase activity and high and low shoot dry weight after evaluation at three dates and two locations in each of 2 years (F5 and F6 generations). This study's objectives were to evaluate the N2-fixing ability of the selected lines and to evaluate the association between plant growth habit and N2 fixation. Twenty-four lines in each of the four selection groups and the parents, NC 6 and 922, were evaluated at two sampling dates and two locations. Mean nitrogenase activity of lines selected for increased nitrogenase activity was significantly greater than the mean of the lines selected for low nitrogenase activity. Improved nitrogenase activity was associated with increased fruit weight. The fruit weight mean of the group selected for increased fruit weight. The fruit weight mean of the group selected for increased nitrogenase activity was 39% greater than the mean of the group selected for low nitrogenase activity. Mean shoot dry weight of lines selected for increased shoot dry weight was significantly greater than the mean of the lines selected for low shoot dry weight; however, the fruit weight means of these two groups did not differ. It was hypothesized that selection for increased N2 fixation in a population derived from a cross between Virginia and Spanish types would eliminate genotypes with Spanish growth habit. Groups selected for high nitrogenase activity and for high shoot dry weight had longer and wider leaflets, longer cotyledonary laterals and greater main stem height than did their respective low selection groups. However, these traits chosen to characterize plant growth habit were inadequate in discriminating parental growth habits. Consequently, the data neither substantiated nor refuted the hypothesis.

Inheritance of Symbiotic Nitrogen Fixation in Two Peanut Crosses1

1989 ◽  
Vol 16 (2) ◽  
pp. 66-70 ◽  
Author(s):  
T. D. Phillips ◽  
J. C. Wynne ◽  
G. H. Elkan ◽  
T. J. Schneeweis
Keyword(s):  
Nitrogen Fixation ◽  
Symbiotic Nitrogen Fixation ◽  
Specific Activity ◽  
Nitrogenase Activity ◽  
Dry Weight ◽  
Dominance Model ◽  
Nodule Number ◽  
Shoot Dry Weight ◽  
Arachis Hypogaea L ◽  
Reciprocal Differences

Abstract Symbiotic nitrogen fixation in peanut (Arachis hypogaea L.) may be improved by genetically manipulating the host plant. This requires an understanding of the inheritance of the traits involved in nitrogen fixation. The objectives of this study were to determine the inheritance of several N2 fixation-related traits for two peanut crosses based on Mather and Jink's fixation-related traits for two peanut crosses based on Mather and Jink's additive-dominance model, and to determine if epistasis was important in the inheritance of these traits. A generation means analysis usingparents, reciprocal F1s and F2s, and two back-cross generations was conducted for both crosses. Plants of different generations were grown in modified Leonard jars in the greenhouse for about 60 days at which time nodule number and dry weight, shoot dry weight, nitrogenase activity, and specific activity were measured. Means of the traits for the generations from both crosses (Robut 33-1 x NC 4 and Robut 33-1 x Argentine) showed significant differences. Reciprocal differences were found for most traits measured in the cross of Robut 33-1 x Argentine, a cross of Virginia x Spanish botanical types. Lack of fit of the additive-dominance model indicated significant epistasis for inheritance of nodule number, nodule weight, top dry weight, and nitrogenase activity in both crosses. Three types of digenic interactions (additive x additive, additive x dominance and dominance x dominance) were found. The presence of nonadditive genetic effects suggests that early generation selection would be ineffective.

scholarly journals Applications of plant growth promoting bacteria and Trichoderma spp. for controlling Orobanche crenata in faba bean

2020 ◽  
Vol 44 (1) ◽  
Author(s):  
Mahmoud Ahmed Touny El-Dabaa ◽  
Hassan Abd-El-Khair
Keyword(s):  
Plant Growth ◽  
Faba Bean ◽  
Growth Parameters ◽  
Dry Weight ◽  
Research Centre ◽  
Plant Growth Promoting Bacteria ◽  
Fresh Weight ◽  
Orobanche Crenata ◽  
Trichoderma Spp ◽  
Shoot Dry Weight

Abstract Background Orobanche crenata is an obligate root parasite belonging to Orbanchaceae. Broomrape causes great damage to the faba bean. Several attempts were applied for controlling parasitic weeds. So, the aim of this work is to study the application of Trichoderma spp. as well as three rhizobacteria species in comparison to herbicidal effect of Glyphosate (Glialka 48% WSC) for controlling broomrape infesting faba bean (Vicia faba). Materials and methods Three pot experiments were carried out in the greenhouse of the National Research Centre, Dokki, Giza, Egypt during two successive winter seasons. Trichoderma inocula were adjusted to 3.6 × 108 propagules/ml and the bacterium inocula were adjusted at 107–109 colony-forming unit (CFU)/ml. All treatments were applied, before 1 week of sowing, at rate of 50 ml per pot in experiments I and II, while 100 ml per pot in experiment III. Results Trichoderma spp. (T. harzianum, T. viride and T. vierns) as well as three rhizobacteria species (Pseudomonas fluorescens, Bacillus subtilis and Bacillus pumilus) enhanced the growth parameters in faba bean plants, i.e. shoot length, shoot fresh weight, shoot dry weight and leaf number in the first experiment when applied without O. crenata infection. In the second experiment, all bio-control could protect plants against O. crenata infection, where it had better juvenile number reduction, than glyphosate after 2 months of application. Both B. subtilis and B. pumilus had the highest reduction to juvenile fresh weight, while their effect was equal to herbicide for juvenile dry weight, respectively. The bio-control agents had high effects until the 4th month, but it was less than that of the herbicide. In experiment III, the bio-control agents could highly reduce the juvenile parameters after 2 months, as well as juvenile fresh weight and juvenile dry weight after 4 months, than the herbicide, respectively. The bio-control agents were effective until 6 months, but less than the herbicide effect. All bio-control treatments highly increased the plant growth parameters, than the herbicide. Conclusion The application of Trichoderma spp. as well as rhizobacteria species could play an important role in controlling broomrape in faba bean as a natural bioherbicide.

High-temperature-adaptedAzospirillum brasilensestrains: growth and interaction response on associative nitrogen fixation, mineral uptake and yield of cheena (Panicum miliaceumL.) genotypes in calcareous soil

1988 ◽  
Vol 110 (2) ◽  
pp. 321-329 ◽  
Author(s):  
R. Rai
Keyword(s):  
Nitrogen Fixation ◽  
High Temperature ◽  
Calcareous Soil ◽  
Nitrogenase Activity ◽  
Dry Weight ◽  
Cross Resistance ◽  
Mineral Uptake ◽  
Associative Nitrogen Fixation ◽  
Seed Inoculation ◽  
Resistance To Penicillin

SummaryHigh-temperature-adapted strains RAU 1, RAU 2 and RAU 3 ofAzospirillum brasilenseC 7 were isolated from stepwise transfer to higher temperature (30 to 42 °C). One of the strains (RAU 1) showed more growth, greater nitrogenase and hydrogenase activities at 30 and 42 °C than parental and other temperature-adapted strains. This strain also showed growth and more nitrogenase activity from pH 6·5 to 8·0. Strain RAU 1 showed cross-resistance to penicillin (300/µg/ml) but not to streptomycin, kanamycin, viomycin and polymixin B at 30 and 42 °C. It was demonstrated in field plots in calcareous soil that seed inoculation with RAU 1 enhanced mineral uptake of cheena. Inoculation with RAU 1 led to a significant increase in associative nitrogen fixation, dry weight of roots, grain and straw yield of cheena compared with the uninoculated control with or without applied N, but the effect of seed inoculation with high-temperature-adapted strains was variable with different genotypes of cheena.

scholarly journals The Root Endophytic Fungus Serendipita indica Decreased the Phytotoxicity of Zinc Oxide Nanoparticles to Alfalfa (Medicago sativa L.)

2021 ◽  
Author(s):  
Leila Tabande ◽  
Mozhgan Sepehri ◽  
Jafar Yasrebi ◽  
Mehdi Zarei ◽  
Reza Ghasemi-Fasaei ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Plant Growth ◽  
Zinc Oxide Nanoparticles ◽  
Sinorhizobium Meliloti ◽  
Dry Weight ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
Shoot Dry Weight ◽  
Nano Zinc Oxide ◽  
Nano Zinc

Abstract Zinc oxide nanoparticles (ZnO-NPs) are among the most commonly used nano-fertilizers (NF). However, elevated levels of ZnO-NPs in soil may affect plant growth and development due to its potential toxicity when accumulated in large amounts in plant tissues. This research was conducted using an in situ rhizobox system with the aims of evaluating Zinc uptake from nano-zinc oxide amended rhizosphere soil by alfalfa plant and the effect of plant growth promoting microorganisms on alleviating the phytotoxicity of ZnO-NPs. Treatments included microbial inoculations (Sinorhizobium meliloti, Serendipita indica) and different ZnO-NPs concentrations (0, 400 and 800 mg Kg− 1) with three replications. The results indicated that S. indica minimized the phytotoxicity of ZnO-NPs to alfalfa by enhancing growth rate and decreasing Zinc (Zn) translocation from root to shoot. Compared with plants inoculated with S. meliloti, co-inoculation with S. indica increased the shoot dry weight by 18.33% and 8.05% at 400 and 800 mg Kg− 1ZnO-NPs. However, at the highest level of ZnO-NPs (800 mg kg− 1), root inoculation of S. indica and S. indica + S. meliloti decreased Zn transfer factor by 60.2% and 44.3% compared to S. meliloti, respectively. Furthermore, a distinct relation between tolerance of S. indica-colonized plant to ZnO-NPs and the ability of S. indica in inhibiting or retarding degradation of polyunsaturated lipids through prevention of excess reactive oxygen species formation was observed. Malondialdehyde content of inoculated plants with S. indica either alone or in combination with S. meliloti was significantly lower than non-inoculated plants (p < 0.01). Zn-induced oxidative stress was mitigated by S. indica through enhanced activities of catalase and peroxidase enzymes. The findings of the present study indicate the potential use of endophytes fungus S. indica for ensuring food safety and security, and human health in heavy metal–polluted soil by reducing the phytoavailability of heavy metals in the aerial parts of the host plants.

Nitrogen Fixation on the Tropical Volcano, La Soufrière (Guadeloupe): Nitrogen Fixation, Photosynthesis and Respiration During the Prevailing Cloud/Shroud Climate By Stereocaulon Virgatum

1988 ◽  
Vol 20 (1) ◽  
pp. 41-61 ◽  
Author(s):  
R. P. Fritz-Sheridan ◽  
D. S. Coxson
Keyword(s):  
Nitrogen Fixation ◽  
Ethylene Production ◽  
Nitrogenase Activity ◽  
Lava Flows ◽  
The West ◽  
Recovery Pattern ◽  
Light Intensities ◽  
Major Variable ◽  
The Mean ◽  
Photosynthesis And Respiration

AbstractStereocaulon virgatum Ach. has colonized lava flows deposited on the west flank of the volcano La Soufrière. The mean annual rate of acetylene reduction was 43·4 nmol C2H4 gdw−1 h−1 with maximum rates during the prevalent cloud/shroud meteorology of 101 and minimum rates during rare high insolation events of 0·63 nmol gdw−1 h−1. Percentage thallus moisture was the major variable controlling nitrogenase activity. During cloud/shroud conditions the upper 90% of the lichen canopy reduced 85% of the acetylene. Canopy shading reduced intra-canopy temperatures allowing the basal 10% of the canopy to fix nitrogen during insolation shocks. Basal portions of pseudopodetia exhibited reduced rates of ethylene production when exposed to canopy surface light intensities during cloud/shroud conditions. The recovery pattern of nitrogenase following desiccation during an insolation shock is presented. Rates of photosynthesis during cloud/shroud conditions were high, reaching 50% of those attained during saturating light intensities.

scholarly journals Grafted eggplant yield, quality and growth in infested soil with Verticillium dahliae and Meloidogyne incognita

2009 ◽  
Vol 44 (12) ◽  
pp. 1673-1681 ◽  
Author(s):  
Sebahattin Çürük ◽  
H. Yıldız Dasgan ◽  
Sedat Mansuroğlu ◽  
Şener Kurt ◽  
Meltem Mazmanoğlu ◽  
...  
Keyword(s):  
Plant Height ◽  
Verticillium Dahliae ◽  
Meloidogyne Incognita ◽  
Solanum Melongena ◽  
Dry Weight ◽  
Fruit Weight ◽  
Infested Soil ◽  
Solanum Torvum ◽  
Shoot Dry Weight ◽  
Soil Infestation

The objective of this work was to evaluate the effect of grafting (onto Solanum torvum Sw.) on plant growth, yield and fruit quality of the Pala and Faselis eggplant (Solanum melongena L.) cultivars, grown in a soil infested with Verticillium dahliae Kleb. and Meloidogyne incognita, or in noninfested soil. Soil infestation decreased yield, plant height, final above-ground biomass, and also reduced fruit mean weight and shoot dry weight depending on cultivar or grafting. Grafting decreased fruit oxalic acid and the soluble solid contents, and increased mean fruit weight, depending on cultivar and soil infestation. Grafting also reduced the negative effects of the pathogens on disease index, plant height and shoot dry weight. Cultivar Pala was more vigorous than Faselis, and S. torvum was a vigorous rootstock. The combination of a vigorous rootstock with a weak cultivar (Faselis) is more profitable than that of a vigorous rootstock and a vigorous cultivar (Pala). Using S. torvum as a rootstock for cultivar Faselis, grown in soil infested with the pathogens, is most likely to be useful in conventional and low-input sustainable horticulture, since grafting increases protection against the pathogens, and reduces the losses in quality and yield.

Effect of weed management on weeds, and on the nodulation, nitrogenase activity, growth and yield of pea (Pisum sativum)

2006 ◽  
Vol 54 (4) ◽  
pp. 469-485 ◽  
Author(s):  
G. Singh ◽  
D. Wright
Keyword(s):  
Pisum Sativum ◽  
Nitrogen Content ◽  
Weed Management ◽  
Plant Biomass ◽  
Nitrogenase Activity ◽  
Strong Relationship ◽  
Dry Weight ◽  
Growth And Yield ◽  
N Content ◽  
Shoot Dry Weight

Effects of one pre-emergence herbicide (terbutryn/terbuthylazine) and one post-emergence herbicide (bentazone) along with unweeded and hand-weeded controls on weeds and on the nodulation, nitrogenase activity, nitrogen content, growth and yield of pea (Pisum sativum) were studied. Terbutryn/terbuthylazine was applied pre-emergence @ 1.40, 2.80 and 5.60 kg/hawhereas bentazone was sprayed 6 weeks after sowing @ 1.44, 2.88 and 5.76 kg/h. Terbutryn/terbuthylazine controlled all the weeds very effectively, whereas bentazone did not control some weeds such as Polygonum aviculare, Poa annua and Elymus repens. The herbicides decreased the number of nodules, the dry weight of nodules, the nitrogenase activity, the shoot dry weight, the nitrogen content in the straw and seeds, and the seed yield of peas, the effects generally being higher at higher rates of application. The adverse effects of herbicides on these parameters might be due to their effects on plant growth, as both the herbicides are known to adversely affect photosynthesis. Nitrogenase activity did not correlate well with plant-N content or shoot dry weight. However, there was a strong relationship between plant biomass and plant-N content, which suggests that researchers can rely on these parameters for studying the effects of treatments on nitrogen fixation, rather than measuring nitrogenase activity.

scholarly journals Root Microbiome Modulates Plant Growth Promotion Induced by Low Doses of Glyphosate

mSphere ◽  
2020 ◽  
Vol 5 (4) ◽  
Author(s):  
Dario X. Ramirez-Villacis ◽  
Omri M. Finkel ◽  
Isai Salas-González ◽  
Connor R. Fitzpatrick ◽  
Jeffery L. Dangl ◽  
...  
Keyword(s):  
Plant Growth ◽  
Root Growth ◽  
Growth Inhibition ◽  
Growth Promotion ◽  
Action Spectrum ◽  
Dry Weight ◽  
Agricultural Fields ◽  
Low Doses ◽  
Shoot Dry Weight ◽  
Root Microbiome

ABSTRACT Glyphosate is a commonly used herbicide with a broad action spectrum. However, at sublethal doses, glyphosate can induce plant growth, a phenomenon known as hormesis. Most glyphosate hormesis studies have been performed under microbe-free or reduced-microbial-diversity conditions; only a few were performed in open systems or agricultural fields, which include a higher diversity of soil microorganisms. Here, we investigated how microbes affect the hormesis induced by low doses of glyphosate. To this end, we used Arabidopsis thaliana and a well-characterized synthetic bacterial community of 185 strains (SynCom) that mimics the root-associated microbiome of Arabidopsis. We found that a dose of 3.6 × 10−6 g acid equivalent/liter (low dose of glyphosate, or LDG) produced an ∼14% increase in the shoot dry weight (i.e., hormesis) of uninoculated plants. Unexpectedly, in plants inoculated with the SynCom, LDG reduced shoot dry weight by ∼17%. We found that LDG enriched two Firmicutes and two Burkholderia strains in the roots. These specific strains are known to act as root growth inhibitors (RGI) in monoassociation assays. We tested the link between RGI and shoot dry weight reduction in LDG by assembling a new synthetic community lacking RGI strains. Dropping RGI strains out of the community restored growth induction by LDG. Finally, we showed that individual RGI strains from a few specific phyla were sufficient to switch the response to LDG from growth promotion to growth inhibition. Our results indicate that glyphosate hormesis was completely dependent on the root microbiome composition, specifically on the presence of root growth inhibitor strains. IMPORTANCE Since the introduction of glyphosate-resistant crops, glyphosate has become the most common and widely used herbicide around the world. Due to its intensive use and ability to bind to soil particles, it can be found at low concentrations in the environment. The effect of these remnants of glyphosate in plants has not been broadly studied; however, glyphosate 1,000 to 100,000 times less concentrated than the recommended field dose promoted growth in several species in laboratory and greenhouse experiments. However, this effect is rarely observed in agricultural fields, where complex communities of microbes have a central role in the way plants respond to external cues. Our study reveals how root-associated bacteria modulate the responses of Arabidopsis to low doses of glyphosate, shifting between growth promotion and growth inhibition.

scholarly journals Genome-Wide Association Mapping of Prostrate/Erect Growth Habit in Winter Durum Wheat

2020 ◽  
Vol 21 (2) ◽  
pp. 394 ◽  
Author(s):  
Daniela Marone ◽  
Monica Rodriguez ◽  
Sergio Saia ◽  
Roberto Papa ◽  
Domenico Rau ◽  
...  
Keyword(s):  
Plant Growth ◽  
Association Mapping ◽  
Durum Wheat ◽  
Candidate Genes ◽  
Zinc Finger ◽  
Growth Habit ◽  
Genome Wide Association ◽  
Genome Wide ◽  
Plant Growth Habit ◽  
Tiller Angle

By selecting for prostrate growth habit of the juvenile phase of the cycle, durum wheat cultivars could be developed with improved competitive ability against weeds, and better soil coverage to reduce the soil water lost by evaporation. A panel of 184 durum wheat (Triticum turgidum subsp. durum) genotypes, previously genotyped with DArT-seq markers, was used to perform association mapping analysis of prostrate/erect growth habit trait and to identify candidate genes. Phenotypic data of plant growth habit were recorded during three consecutive growing seasons (2014–2016), two different growth conditions (field trial and greenhouse) and two sowing periods (autumn and spring). Genome-wide association study revealed significant marker-trait associations, twelve of which were specific for a single environment/year, 4 consistent in two environments, and two MTAs for the LSmeans were identified across all environments, on chromosomes 2B and 5A. The co-localization of some MTAs identified in this study with known vernalization and photoperiod genes demonstrated that the sensitivity to vernalization and photoperiod response are actually not only key components of spring/winter growth habit, but they play also an important role in defining the magnitude of the tiller angle during the tillering stage. Many zinc-finger transcription factors, such as C2H2 or CCCH-domain zinc finger proteins, known to be involved in plant growth habit and in leaf angle regulation were found as among the most likely candidate genes. The highest numbers of candidate genes putatively related to the trait were found on chromosomes 3A, 4B, 5A and 6A. Moreover, a bioinformatic approach has been considered to search for functional ortholog genes in wheat by using the sequence of rice and barley tiller angle-related genes. The information generated could be used to improve the understanding of the mechanisms that regulate the prostrate/erect growth habit in wheat and the adaptive potential of durum wheat under resource-limited environmental conditions.

Effects of Barnyardgrass (Echinochloa crus-galli) on Growth, Yield, and Nutrient Status of Transplanted Tomato (Lycopersicon esculentum)

Weed Science ◽  
1988 ◽  
Vol 36 (6) ◽  
pp. 775-778 ◽  
Author(s):  
Prasanta C. Bhowmik ◽  
Krishna N. Reddy
Keyword(s):  
Unit Area ◽  
Tomato Fruit ◽  
Growth Yield ◽  
Dry Weight ◽  
Nutrient Status ◽  
Field Studies ◽  
Fruit Weight ◽  
Fresh Weight ◽  
Shoot Dry Weight ◽  
Marketable Fruit

Field studies were conducted to determine the effects of various barnyardgrass populations on growth, yield, and nutrient concentration of transplanted “Jetstar’ tomato. Barnyardgrass densities at 16, 32, and 64 plants/m tomato row were tested in 1982 and 1983. Barnyardgrass shoot fresh weights/unit area increased as density increased. Fresh weight of barnyardgrass shoots ranged from 17 100 kg/ha at 16 plants/m of row to 35 500 kg/ha at 64 plants/m of row. At the vegetative stage, tomato shoot dry weight was unaffected by barnyardgrass. As crop growth progressed, tomato shoot dry weight decreased at all barnyardgrass densities. Season-long interference of barnyardgrass reduced marketable tomato fruit number and fruit weight at all densities compared to weed-free plots. Reductions in marketable fruit weight ranged from 26% to 16 plants/m row to 84% at 64 plants/m row. In 1982, concentrations of N, P, K, Ca, and Mg in tomato shoots were unaffected by season-long interference of barnyardgrass at all densities. However, in 1983, concentrations of N and K decreased and concentration of P increased in tomato leaves as the density of barnyardgrass increased. Concentrations of Ca and Mg in tomato leaves were unaltered by barnyardgrass density.

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