scholarly journals PRODUCTIVITY OF RAINBOW TROUT DEPENDING ON PROTEIN LEVELS IN PRODUCTIVE FEED

2020 ◽  
Vol 11 (4) ◽  
pp. 45-53
Author(s):  
V. M. Kondratiuk

The article considers the efficiency of using complete feeds with different levels of protein in rearing commercial rainbow trout. The purpose of the experiment was to establish the effect of different protein levels in nutrition of two-year old trout on its productivity. For this purpose, five experimental groups were formed by the method of analogues. During the equalizing period, the experimental fish consumed feed of the control group. In the main period, the protein level in trout feed ranged from 44 to 52 % per 1 kg. As a result of the studies it was established that the increase of protein content in compound feed from 48 to 52 % leads to an increase in the weight of commercial fish by 13.0 % (p < 0.001), an increase in its growth intensity – by 7.4-16.7 %, while the reduction of protein in feed to 44 % leads to a reliable (p < 0.05) reduction in trout weight by 9.4 %, and a decrease in its growth intensity - by 6.5-12.1 %. It is proved that different levels of experimental trout’s protein nutrition have a highly reliable (p < 0.001) effect on the growth of marketable fish - the share of this factor’s influence is 89.8 %. Analysis of the feed costs, proves that using combined feed with 52 % protein for feeding two-year old trout, leads to a decrease in feed costs per 1 kg of weight gain by 5.1 %, whereas reducing this index to 44% causes increase of feed costs by 6.4 % per a unit of product, compared to feeding fish with production feed having the protein content of 48 %. At this, the preservation of experimental fish was high enough and made 94.3 to 96.2 %. In rearing trouts according to the criteria of maximum productivity, it is recommended to feed commercial trout with production combined feeds with the protein level of 52 %, whereas, in production according to the economic optimization criteria, this index should make 48 %.

2020 ◽  
Vol 11 (3) ◽  
pp. 34-42
Author(s):  
V. M. Kondratiuk

The article considers the efficacy of using complete feed stuffs with different protein levels for rearing rainbow trout juvenile fish up to 10 g. The purpose of the experiment was to establish the influence of different protein nutrition levels on trout productivity indices. For this purpose, five experimental groups of this year juvenile fish were formed by the method of analogues. The control group of fish consumed feed stuffs with the protein level of 52%. During the comparative period, the experimental fish consumed feed of the control group. In the main period, the level of crude protein in trout feed stuffs ranged from 48 to 56% per 1 kg. As a result of the study performed, it was found that the use of feed stuffs with high protein nutrition (56 %) in feeding of this year trout is accompanied by an increase in their weight by 12.3 % (p < 0.01) and their growth intensity - by 7.1-15.7 % , compared to the analogues who consumed feed with the protein level of 52 %. Reduction of this index to the level of 48% contributes to a probable reduction (p < 0.05) in weight by 11.5 %, and a decrease in growth intensity by 5.8-13.8 %. It has been proved that the cost of feed per 1 kg of weight gain in this year trout, which consumed feed stuff with a protein content of 56 %, was lower by 5.5 %, and with its content at 48 % - by 7.4 % higher, compared to fish that consumed feed stuff with the protein content of 52 %. At the same time, the safety of the experimental fish throughout the experiment was compliant with the normative indices in trout farming and was within the range of 75.2-78.1 %. The analysis of the obtained fish farming results showed that the most economically feasible is rearing of this year trout juvenile fish, who consume feed stuff with the protein nutritional value of 52 %, compared to a decrease or increase in this index to 48 and 56 %, respectively.


2020 ◽  
Vol 11 (2) ◽  
pp. 48-55
Author(s):  
V. M. Kondratiuk

The article considers the effectiveness of the use of mixed feeds with different levels of metabolic energy for the cultivation of larvae and fry of rainbow trout. The aim of the experiment was to establish the influence of different levels of energy supply of trout on its performance. For this purpose, five experimental groups were formed by the method of analogues. During the equalization period, the experimental fish consumed feed of the control group. In the main period, the level of metabolic energy in trout feed ranged from 14 to 18 MJ. As a result of the conducted researches it is established that the increase of energy content in compound feed for trout larvae and fry from 16 to 18 MJ contributes to the increase of their weight by 11.1 % (p < 0.05) and growth intensity - by 5.6-14.2 %, while the reduction of energy content to 14 MJ / kg contributes to a probable decrease (p < 0.05) in mass by 11.9%, and a decrease in growth intensity by 6.0-14.7 %. It is proved that the feed consumption per 1 kg of weight gain in trout larvae and fry, which received feed with an energy content of 18 MJ was lower by 5.1 %, and with its content of 14 MJ - 4.8 % higher compared to fish consuming feed with an energy content of 16 MJ / kg. The safety of the experimental fish throughout the experiment was close and ranged from 83.1 to 84.9 %. However, it is most economically feasible to grow trout that consume feed with a nutritional value of 16 MJ of metabolic energy, compared with a decrease or increase of this indicator to 14 or 18 MJ, respectively.


Author(s):  
Vadym Kondratiuk

The article considers the effectiveness of complete feed with different levels of lysine and methionine for growing rainbow trout up to 10 g. The experiment aimed to establish the effect of different levels of amino acid nutrition of trout on its productivity. For this purpose, five experimental groups of this year were formed by the method of analogues. The experiment lasted 45 days and was divided into two periods: equalization (5 days) and main (40 days). During the equalization period, the experimental fish consumed feed of the control group. In the main period, the level of lysine and methionine in experimental feeds for different experimental groups of trout ranged from 2.8 to 3.2 % and from 0.90 to 1.10 %, respectively. As a result of research, it was found that the use of trout weighing up to 10 g of feed with high amino acid nutrition (3.1 % lysine, 1.05 % methionine) in the feeding of this year is accompanied by an increase in their weight by 9.2 % (p <0.05) and growth intensity - by 4.1-9.3 %, compared with analogues who consumed feed with the content of lysine of 3.0% and methionine of 1.0%. The reduction of these amino acids to the levels of 2.8 and 0.9 %, respectively, contributes to a probable decrease (p <0.01) in the weight of fish by 14.1 %, and a decrease in growth intensity by 8.7-17.3 %. It was proved that different levels of amino acid nutrition of young trout significantly (p <0.01) affected the weight of experimental fish. The share of influence of this factor is 61.4 %, which is 22.8 % more than the influence of other factors. In the course of research, it was determined that when growing rainbow trout this year up to 10 g, feeding them compound feeds with lysine and methionine content of 3.1 and 1.05% helps to reduce feed costs per unit weight gain by 12.5 %, compared with using feeds with levels of these amino acids of 2.8 and 0.9 %, respectively. At the same time, the safety of the experimental fish throughout the experiment corresponded to the normative indicators in trout farming and was in the range of 76.0-77.3 %. The analysis of the obtained fish farming results showed that for the production of trout products according to the indicators of maximum productivity and economic optimization criteria it is recommended to use complete feed with a level of 3.1% lysine and 1.05% methionine for feeding rainbow trout.


2000 ◽  
Vol 80 (4) ◽  
pp. 633-642 ◽  
Author(s):  
Palle V. Rasmussen ◽  
Christian F. Børsting

The effect of different and shifting dietary protein levels on hair growth and the resulting pelt quality in mink was studied. Two groups of pastel female mink were fed either 59% (high protein, HP) or 40% (low protein, LP) of metabolisable energy (ME) from protein during pregnancy and lactation. Shortly after weaning, kits from females fed the LP diet were put on a new LP diet (21% protein of ME). Kits from females fed HP were randomly distributed to four experimental groups fed a new HP diet (34% protein of ME) and three of these groups were shifted to diets with 21% protein at different times during June until September. Skin biopsies were taken at 4, 6, 23 and, 29 wk of age. Histological techniques and computer-assisted light microscopy were used to determine the ratio of activity (ROA) of underfur and guard hairs, respectively, defined as the number of growing hairs as a percentage of the total number of hairs. The hair fibre length and thickness were determined by morphometric methods and correlated with fur properties of dried pelts judged by sensory methods. It was documented that 40% of ME from protein during pregnancy and lactation was sufficient for mink kits to express their genetic capacity to produce hair follicles. In males, a reduced protein level from the age of 15 wk or 22 wk until pelting disturbed moulting, indicated by a low ROA of underfur hairs at 23 wk, and consequently reduced the growth and development of the winter coat. A constantly low protein level from conception until the age of 29 wk did not disturb moulting, but led to a reduction of primeness and especially of the underfur length and fibre thickness of the winter coat. A low protein level from the age of 9 wk only reduced the thickness of the underfur fibres. Hair growth, final fur volume, and general quality of the winter coat of males were influenced negatively and to the same degree in all groups fed the LP diet in part of the growth period. The number of underfur hairs per area (hair density) of the winter coat was not influenced by the dietary treatment meaning that the protein content of 21% of ME in the LP diet was high enough for the mink to express its genetic capacity to develop hair follicles. However, this low protein content led to a reduction of hair fibre length and hair fibre thickness of the underfur. Overall, this study demonstrated that hair growth and hair properties in pelts are very dependent on the dietary protein supply in the period from 22 wk of age until pelting, irrespective of the supply in the preceding periods. Key words: Fur properties, hair fibres, nutrition, pelage, protein requirement


Author(s):  
V. Kondratiuk ◽  

The article considers the efficiency of using complete compound feeds with different levels of lysine and methionine in rearing larvae and juveniles of rainbow trout. The purpose of the experiment was to establish the effect of different amino acid levels in nutrition of juvenile trout on its productivity. For this purpose, five experimental groups were formed by the method of analogues. The experiment lasted 35 days and was divided into two periods: equalizing (5 days) and main (30 days). During the equalizing period, the experimental fish consumed feed of the control group. In the main period, the levels of lysine and methionine in experimental feeds for different experimental groups of trout ranged from 2.9 to 3.3% and from 0.95 to 3.15%, respectively. It is proved that increasing the content of lysine and methionine in feed for larvae and juveniles of rainbow trout to the levels of 3.2 and 1.1%, respectively, promotes increase in their weight by 10.7% (p <0.01) and growth intensity - by 6.4-11.1%, while further increase in these amino acids content in the feed reduces the productivity of fish. Polynomial equations for determining the juvenile fish weight at any stage of rearing at a high level of determination for each of the experimental groups have been calculated. It was found that with an increase in the content of lysine and methionine in feed to the levels of 3.2 and 1.1%, respectively, reduces feed costs per 1 kg of weight gain in trout larvae and juveniles by 4.1%, and a further increase in levels of these amino acids to 3.3 and 1.15%, respectively, increase this index by 1.1%. The experimental fish survival ranged from 83.9 to 85.1%. In the production of trout according to the criteria of maximum productivity and to the economic optimization criteria, it is recommended to use complete feed with the level of lysine - 3.2% and methionine - 1.1% for feeding trout larvae and juveniles.


2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 405-405
Author(s):  
Vinícius C De Souza ◽  
Juliana Messana ◽  
Erick Batista ◽  
Paulo Henrique Colovate ◽  
Maria Júlia Ganga ◽  
...  

Abstract The goal of this study was to evaluate the effects of different levels (10 and 13% crude protein) and protein source on ruminal fermentation of feedlot Nellore steers fed high-concentrate diets. The protein sources tested were urea, dried distillers grains (DDG) and corn gluten meal. The DDG and gluten were used as RUP sources. We used 6 cannulated Nellore steers, rumen, duodenum, and ileum arranged in 6×6 Latin square designed in factorial (2 nitrogen levels and 3 protein sources) balanced for residues. The pH was measured immediately after rumen fluid sampling at 0, 2, 4, 6, 8 and 10h after feeding. The NH3-N concentrations were determined with micro-Kjeldahl apparatus. The concentration of volatile fatty acids (VFA) was assessed by gas chromatography. Data were evaluated using the MIXED procedure of SAS, with repeated measures over time. There was no interaction between sampling time and treatments for NH3-N and pH (P &gt; 0.05). Animals fed diets containing Urea had a higher concentration of NH3-N (20.6 mg/dL) than animals supplemented with Gluten (16.2 mg/dL) or DDG (12.7 mg / dL). There was an increase in the NH3-N concentration (P = 0.049) with the increase of the protein level 10 to 13% CP with 14.7 and 18.3 mg/dL, respectively. The ruminal pH was affected by the dietary protein level (P = 0.016), in which animals fed a level of 10% CP had a higher pH (6.80) compared to the level of 13% CP (6.43). Total and individual VFA concentration was not affected by protein levels and sources (P &gt; 0.05). The reduction of N level and the use of RUP did not adversely affect the ruminal fermentation. Although significant differences were observed for pH, they were small and adequate for microbial protein synthesis in the rumen.


2017 ◽  
Vol 119 (2) ◽  
pp. 131-142
Author(s):  
María E. Oliva ◽  
Agustina Creus ◽  
María R. Ferreira ◽  
Adriana Chicco ◽  
Yolanda B. Lombardo

AbstractThis study investigates the effects of replacing dietary casein by soya protein on the underlying mechanisms involved in the impaired metabolic fate of glucose and lipid metabolisms in the heart of dyslipidaemic rats chronically fed (8 months) a sucrose-rich (62·5 %) diet (SRD). To test this hypothesis, Wistar rats were fed an SRD for 4 months. From months 4 to 8, half the animals continued with the SRD and the other half were fed an SRD in which casein was substituted by soya. The control group received a diet with maize starch as the carbohydrate source. Compared with the SRD-fed group, the following results were obtained. First, soya protein significantly (P<0·001) reduced the plasma NEFA levels and normalised dyslipidaemia and glucose homoeostasis, improving insulin resistance. The protein levels of fatty acid translocase at basal state and under insulin stimulation and the protein levels and activity of muscle-type carnitine palmitoyltransferase 1 were normalised. Second, a significant (P<0·001) reduction of TAG, long-chain acyl CoA and diacylglycerol levels was observed in the heart muscle. Third, soya protein significantly increased (P<0·01) GLUT4 protein level under insulin stimulation and normalised glucose phosphorylation and oxidation. A reduction of phosphorylated AMP protein kinase protein level was recorded without changes in uncoupling protein 2 and PPARα. Fourth, hydroxyproline concentration decreased in the left ventricle and hypertension was normalised. The new information provided shows the beneficial effects of soya protein upon the altered pathways of glucose and lipid metabolism in the heart muscle of this rat model.


1968 ◽  
Vol 19 (2) ◽  
pp. 203
Author(s):  
KB Balaam ◽  
B Dettmann ◽  
HJ Moss

The influence of dough-moulding techniques on the volumes of test loaves has been investigated. The effects of variety of wheat, locality of growth, and season have also been studied. Because of the strong influence of variety, locality, and seasonal conditions on the protein content of wheat and flour, and in turn the effect of protein level on loaf volume and baking quality, an analysis of covariance with protein content was performed. Where protein levels in the flour were high, hand-moulded loaves had greater volume than those moulded by machine, the reverse being the case where protein levels were low. Flours of Spica variety tended to be overrated and of Heron variety underrated by hand moulding relative to machine moulding but the manner of moulding did not affect the overall ranking of varieties. Variety, season, and locality each influenced volume even after allowing for the effect of protein level. Varieties should be evaluated over a range of environments because of variability from trial to trial.


1968 ◽  
Vol 8 (30) ◽  
pp. 85 ◽  
Author(s):  
HJ Moss

Mottling in wheat is the condition whereby starchy and vitreous sections are found together in a grain or a sample. The occurrence of mottling in nine varieties was studied in relation to flour protein content, proportion of large starch granules, and gluten strength. When protein levels fell below 10 per cent the varieties differed in mottling behaviour. Some varieties lost vitreousness but became opaque rather than mottled; others became mottled. It was concluded that although low protein level is a predisposing factor, mottling is aggravated by factors associated with high dough stability and a high proportion of large starch granules. The interaction of these factors is used to explain the differing susceptibilities of varieties to mottling.


Nutrients ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 2529
Author(s):  
Anna Rafało-Ulińska ◽  
Ewa Poleszak ◽  
Aleksandra Szopa ◽  
Anna Serefko ◽  
Magdalena Rogowska ◽  
...  

Zinc (Zn) was found to enhance the antidepressant efficacy of imipramine (IMI) in human depression and animal tests/models of depression. However, the underlying mechanism for this effect remains unknown. We measured the effect of intragastric (p.o.) combined administration of IMI (60 mg/kg) and Zn (40 mg Zn/kg) in the forced swim test (FST) in mice. The effect of Zn + IMI on serum, brain, and intestinal Zn concentrations; Zn transporter (ZnT, ZIP) protein levels in the intestine and ZnT in the brain; including BDNF (brain-derived neurotrophic factor) and CREB (cAMP response element-binding protein) protein levels in the brain were evaluated. Finally, the effect of IMI on Zn permeability was measured in vitro in colon epithelial Caco-2 cells. The co-administration of IMI and Zn induced antidepressant-like activity in the FST in mice compared to controls and Zn or IMI given alone. This effect correlated with increased BDNF and the ratio of pCREB/CREB protein levels in the prefrontal cortex (PFC) compared to the control group. Zn + IMI co-treatment increased Zn concentrations in the serum and brain compared to the control group. However, in serum, co-administration of IMI and Zn decreased Zn concentration compared to Zn alone treatment. Also, there was a reduction in the Zn-induced enhancement of ZnT1 protein level in the small intestine. Zn + IMI also induced an increase in the ZnT4 protein level in the PFC compared to the control group and normalized the Zn-induced decrease in the ZnT1 protein level in the hippocampus (Hp). The in vitro studies revealed enhanced Zn permeability (observed as the increased transfer of Zn through the intestinal cell membrane) after IMI treatment. Our data indicate that IMI enhances Zn transfer through the intestinal tract and influences the redistribution of Zn between the blood and brain. These mechanisms might explain the enhanced antidepressant efficacy of combined IMI/Zn treatment observed in the FST in mice.


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