Examining the Expression of miR-205 and CEA mRNA in Peripheral Blood of Patients with OSCC(Oral Squamous Cell Carcinomas) and Comparing them with Healthy People

Introduction: Cancer is one of the most important causes of mortality in the world. So, in this study the changes of expressing miR-205 and CEA in oral cancer in peripheral blood were examined for early detection and better treatment. Methods: In this study, we selected the number of 30 patient people and 30 healthy people. We measured their blood miR-205 and CEA using Real-Time PCR technique and evaluated the relationship between the expression of these biomarkers with tumor staging and cancer progression. Findings: there is no a significant difference in mean age by comparing these two groups using t-test. The CEA mRNA biomarker was positive in 24 out of 30 people of the patient people group and was positive in 4 out of 30 people of the healthy people group. Statistical comparison represented a statistically significant difference between the two groups (P-value <0.001). The miR-205 biomarker was positive in 9 out of 30 people of the patient people group and was positive in 22 out of 30 people of the healthy people group. Statistical comparison represented a statistically significant difference the two groups (P-value <0.001). Conclusion: In general, the research result can be considered as a screening test for early detection of the disease in the early stages. It is recommended to conduct more extensive studies with larger sample sizes to further proof of the research results.

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Over-expression of IL-6 coding gene in the peripheral blood of migraine with aura patients

Human Antibodies ◽

10.3233/hab-210442 ◽

2021 ◽

pp. 1-5

Author(s):

Mahdi Ramezani ◽

Alireza Komaki ◽

Mohammad Mahdi Eftekharian ◽

Mehrdokht Mazdeh ◽

Soudeh Ghafouri-Fard

Keyword(s):

Migraine With Aura ◽

Peripheral Blood ◽

Healthy Subjects ◽

Migraine Without Aura ◽

P Value ◽

Healthy Controls ◽

Over Expression ◽

Significant Difference ◽

Male Patients ◽

Years Lived With Disability

Migraine is a common disorder which is placed among the top ten reasons of years lived with disability. Cytokines are among the molecules that contribute in the pathophysiology of migraine. In the current study, we evaluated expression levels of IL-6 coding gene in the peripheral blood of 120 migraine patients (54 migraine without aura and 66 migraine with aura patients) and 40 healthy subjects. No significant difference was detected in expression of IL-6 between total migraine patients and healthy controls (Posterior beta = 0.253, P value = 0.199). The interaction effect between gender and group was significant (Posterior beta =-1.274, P value = 0.011), therefore, we conducted subgroup analysis within gender group. Such analysis revealed that while expression of this gene is not different between male patients and male controls (Posterior beta =-0.371, P value > 0.999), it was significantly over-expressed in female patients compared with female controls (Posterior beta = 0.86, P= 0.002). Expression of IL-6 was significantly higher in patients with aura compared with controls (Posterior beta = 0.63, adjusted P value = 0.019). However, expression of this cytokine coding gene was not different between patients without aura and healthy subjects (Posterior beta = 0.193, adjusted P value = 0.281). Therefore, IL-6 might be involved in the pathophysiology of migraine among females and migraine with aura among both sexes.

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SMOKING GENOTOXICITY IN PERIPHERAL BLOOD LYMPHOCYTES USING THE COMET ASSAY

Journal of Health and Allied Sciences NU ◽

10.1055/s-0040-1703675 ◽

2013 ◽

Vol 03 (03) ◽

pp. 038-041

Author(s):

Shobha S. Shetty ◽

Hrishikesh Nachane

Keyword(s):

Dna Damage ◽

Comet Assay ◽

Peripheral Blood ◽

Peripheral Blood Lymphocytes ◽

The Body ◽

P Value ◽

Tail Moment ◽

Blood Lymphocytes ◽

Significant Difference ◽

Almost All

Abstract Background: Smoking has been shown to have a positive effect on DNA damage in almost all the cells of the body. Quantitative analysis of this damage will help in assessing the etiopathogenesis of various nicotine induced damage to the body. Comet assay has been an emerging tool in this regard and hence was applied by us to estimate the severity of DNA damage in smokers. Aims & Objectives: To evaluate the DNA genotoxicity in peripheral blood lymphocytes in smokers and their comparison with non smokers & assess the quantitative damage. Materials and methods: 30 smokers & 20 non smokers were recruited & their peripheral blood was taken for the comet assay to look for Olive moment & Tail moment to quantitatively assess the DNA damage due to cigarette smoking. Results: In our study there was no significant difference in the analysis of DNA damage (with regard to tail moment & olive moment) in smokers versus non smokers (P value: more than 0.05). Conclusions: Though smoking is known to cause DNA damage, we did not find significant differences between the two groups probably due to other multifactorial etiologies for genotoxicity.

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PEMBERIAN KOMPRES JAHE DALAM MENGURANGI NYERI SENDI PADA LANSIA DI UPT PSTW KHUSNUL KHOTIMAH PEKANBARU

Photon: Jurnal Sain dan Kesehatan ◽

10.37859/jp.v6i02.437 ◽

1970 ◽

Vol 6 (02) ◽

pp. 13-16

Author(s):

Tri Siwi KN

Keyword(s):

Joint Pain ◽

Research Result ◽

The Elderly ◽

Pain Scale ◽

P Value ◽

Average Intensity ◽

Significant Difference ◽

Before And After ◽

Scale Test ◽

Pain In The Elderly

Gout and rheumatoid arthritis is a disease that is often encountered in the community, one of them on the elderly (the elderly) are characterized by pain that occurs repeatedly in the joints. Ginger compress can be used as a nonpharmacological measures to deal with the pain. This study aims to determine the effectiveness of ginger compress in reducing joint pain in the elderly in UPT PSTW Khusnul Khotimah Pekanbaru. This design of research is a quasi experiment with sampling total sampling with 16 respondents. Data collection tool using pain scale test pre and post administration of ginger compress. The research result shows that the average intensity of joint pain before and after the ginger compress is 2.500. The test results obtained statistical p value = 0.000 where p <α 0:05. this means that there are differences in average significant difference between the intensity of joint pain in the elderly before and after the ginger compress. Is expected to more elderly for routine use as a ginger compress nonpharmacological measures pain in the joints.

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Transcript levels of cytokine coding genes in peripheral blood and tissues of patients with periodontitis

Human Antibodies ◽

10.3233/hab-211507 ◽

2021 ◽

pp. 1-9

Author(s):

Mohammad Taheri ◽

Leila Gholami ◽

Fwad Nicknafs ◽

Bashdar Mahmud Hussen ◽

Shahram Arsang-Jang ◽

...

Keyword(s):

Peripheral Blood ◽

Periodontal Diseases ◽

Age Groups ◽

Public Health Problem ◽

Microbial Pathogens ◽

Blood Levels ◽

P Value ◽

Tissue Destruction ◽

Host Immune Responses ◽

Significant Difference

Periodontal diseases are common conditions in almost all age groups and a public health problem. Numerous risk factors have been demonstrated for this condition. The main mechanism of tissue destruction in the periodontitis is the functional interactions between microbial pathogens and host immune responses, thus cytokines have crucial roles in the pathogenesis periodontitis. Our previous study has demonstrated the susceptibility role of HLA-DRB1*04 allele in development of this disease. So, the individuals who were positive for HLA-DRB1*04 allele were excluded. We aimed to appraise the function of cytokines in the pathogenesis of periodontitis via assessment of tissue and blood levels of a number of cytokine coding genes, namely IL-1B, CXCL8, IL-17, IFNG, TGFB and TNFA1. Expressions of IFNG, IL-17, TGFB and TNFA1 were significantly higher in the peripheral blood of individuals with periodontitis compared with unaffected persons (Posterior beta = 1.91, P value = 0.043; Posterior beta = 1.84, P value = 0.033; Posterior beta = 0.713, P value = 0.009 and Posterior beta = 2.85, P value = 0.001, respectively). Moreover, expression of IL-17 was higher in females compared with males (Posterior beta = 1.47, P value = 0.036). As the interaction effect between gender and group was remarkable for IL-17 expression, we further conducted subgroup analysis within gender group. Expression of IL-17 was higher in male patients compared with unaffected males (Posterior beta = 1.9, P value = 0.048). We did not detect any significant difference in the expression of these cytokines in tissues obtained from affected individuals and unaffected controls. Therefore, our results imply dysregulation of cytokine coding genes in patients with periodontitis and warrant further mechanistical studies.

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Enzyme Activity and De Ritis Ratio in Alcoholic and Non Alcoholic Fatty Liver Patients based on Ultrasonography

Journal of Universal College of Medical Sciences ◽

10.3126/jucms.v8i1.29808 ◽

2020 ◽

Vol 8 (1) ◽

pp. 9-13

Author(s):

Sanju Rawal ◽

Aakash Shahi ◽

Narayan Gautam ◽

Archana Jayan ◽

Uday Sharma

Keyword(s):

Enzyme Activity ◽

Liver Disease ◽

Early Detection ◽

Fatty Liver ◽

Fatty Liver Disease ◽

P Value ◽

Alcoholic Fatty Liver ◽

Asymptomatic Patients ◽

Significant Difference ◽

Serum Glutamate

INTRODUCTION: Fatty liver disease (FLD) refers to a wide clinical and histological spectrum from simple hepatic steatosis to steatohepatitis or cirrhosis, and FLD has been classiﬁed as nonalcoholic FLD (NAFLD) and alcoholic FLD (AFLD) based on etiology and ultrasonography (USG). MATERIAL AND METHODS: This Cross-sectional study was undertaken in the Department of Radiology, Universal College of Medical Sciences (UCMS), Bhairahawa, Nepal from March 2019 to February 2020. A total of 100 subjects involved where ultrasonographically graded for fatty liver and enzyme activity were assessed to observe their association. RESULTS: In 100 cases, 66% were male and 34% were female. Ultrasonography (USG) guided FLD grade 1 patients were 81%, FLD grade 2 patients were 19% where as no cases was observed for FLD grade 3. Fifty Six percent (56%) of the total cases presented with AFLD while remaining 44% with NAFLD. There was significant difference in serum glutamate oxaloacetate transaminase (SGOT) (p-value: 0.003), serum glutamate pyruvate transaminase (SGPT) (p-value: 0.011) and alkaline phosphatase (ALP) (p-value: 0.003) in AFLD and NAFLD. However, there was no significant association of enzyme activity with FLD grade (p-value >0.05). There was significant difference in SGOT (p-value: 0.004), SGPT (p- value: 0.025) between AFLD grade 1 (AFLDG1) and NFLD grade 1 (NAFLDG1), SGOT (p-value: 0.016) between AFLDG2 and NAFLDG2 and ALP (p-value: 0.01) between AFLDG1 and NAFLDG1. However, De Ritis ratio was not significantly associated with fatty liver disease. CONCLUSION: USG is a non-invasive simple tool for early detection of fatty liver in asymptomatic patients enabling clinicians to achieve early detection in conjunction with hepatic enzymes.

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PERBEDAAN EFEK MINUMAN BERKARBONASI DENGAN MINUMAN PROBIOTIK TERHADAP PERUBAHAN GAYA Z-SPRING

JURNAL FARMASIMED (JFM) ◽

10.35451/jfm.v3i2.596 ◽

2021 ◽

Vol 3 (2) ◽

pp. 86-92

Author(s):

Jean Saragih ◽

Zulfan Muttaqin ◽

LINA LINA HADI

Keyword(s):

Stainless Steel ◽

Research Result ◽

Control Group ◽

P Value ◽

Orthodontic Wire ◽

Significant Difference ◽

Before And After ◽

Carbonated Beverage ◽

Carbonated Drinks ◽

The Wire

The effect of the drink can cause changes in the resilience of the wire which is usually due to carbonated drinks or probiotics Carbonated beverage is a daily drinks with carbonic acid and has pH of 2.32. Carbonated beverage with lower pH may cause the releasing of nickel (Ni) and chromium (Cr) ions on stainless steel orthodontic wire in oral cavity and result in alteration of resilience. Purpose: The aim of this research is to know the change of resilience of stainless steel removable orthodontic wire before and after immersion with ,probiotic,carbonated beverages and saline solution. Material and methods: This study is true experimental study using pre and post test with control group design, consist of 9 treatment groups and control groups were obtained from preliminary test, each group using stainless steel ortodontic wire with the diameter is 0.6 mm and given a scratch along the wire then perform the resilience before and after the immersion using a UTM. Each sample was immersed and put in an incubator at 37oC for the first 4 days then measured with a UTM device then soaked again for the next 4 days and measured again. Research result: The results showed that there were differences in the resilience of the wires on day 0 and day 4 when immersed in carbonated drinks (p = 0.047) compared to soaking saliva and probiotics. Then z-spring stainless steel orthodontic wire soaked in probiotic obtained p value = 0.038. This shows that there is a difference in the resilience of the 8th day compared to the 0th day.Conclusion: Results Soaking stainless steel orthodontic wire changes its resilience. There was a significant difference between day 4 and day 8 for the wire soaked in carbonated drinks (p = 0.001). there is no significant difference in the wire soaked with probiotic drinks.

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Comparison of Self-Coherence and Impulsiveness between People with Irritable Bowel Syndrome and Healthy People

Social Behavior Research & Health ◽

10.18502/sbrh.v3i1.1038 ◽

2019 ◽

Author(s):

Saeideh Khaki ◽

Hajar Torkan ◽

Vahid Sebghatollahi

Keyword(s):

Irritable Bowel Syndrome ◽

Healthy People ◽

P Value ◽

Third Wave ◽

Healthy Individuals ◽

Social And Emotional ◽

Significant Difference ◽

Self Knowledge ◽

Irritable Bowel ◽

The City

Background: Irritable Bowel Syndrome (IBS) is a serious syndrome, which damages people’s psychological, communicative, social, and emotional lives. Therefore, the present study was conducted to compare integrative self-knowledge and impulsivity in patients with Irritable IBS and healthy individuals. Methods: This case-control study was conducted on patients with IBS referring to gastroenterologists and healthy individuals in the city of Isfahan in 2018. The sample size included 150 patients with IBS and 150 healthy people in Isfahan selected using non-random convenient sampling method. The integrative self-knowledge and impulsivity questionnaires were applied to collect the information. After collecting the questionnaires and extracting the raw data, SPSS23 software was used and t-test was run to analyze the data. Results: A significant difference was observed between patients with IBS and healthy participants with regard to integrative self-knowledge and impulsivity (P-value < 0.001), so that people with IBS had lower integrative self-knowledge and higher impulsivity in comparison with the healthy individuals. Conclusion: According to the findings, people with IBS had lower integrative self-knowledge and higher impulsivity due to their therapeutic and psychological involvement in the disease. So, third-wave psychological treatments such as mindfulness treatment and positivist psychotherapy are required in this area.

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Implementation of Flow Cytometric Monocyte Repartition Assay for Assessment of Chronic Myelomonocytic Leukaemia (CMML) in Bone Marrow

Blood ◽

10.1182/blood-2020-140932 ◽

2020 ◽

Vol 136 (Supplement 1) ◽

pp. 3-4

Author(s):

Sharon A Allen ◽

Eugene Ng ◽

Suzanne Edwards ◽

Uwe H Hahn ◽

Tatjana Banovic ◽

...

Keyword(s):

Bone Marrow ◽

Myeloid Leukaemia ◽

Peripheral Blood ◽

Linear Regression Analysis ◽

Myeloproliferative Neoplasm ◽

P Value ◽

Monocyte Subsets ◽

Roc Curve Analysis ◽

Flow Cytometric ◽

Significant Difference

Background: CMML is a myelodysplastic/myeloproliferative overlap syndrome associated with persistent monocytosis. Previous studies have shown a characteristic phenotype of peripheral blood (PB) monocytes in CMML that may have diagnostic utility. There is expansion of the classical monocyte (Mo1) population (CD14+CD16-) with concomitant reduction in the intermediate (Mo2; CD14+16+) and non-classical (Mo3; CD14-16+) populations. It is unclear whether an increased Mo1 population or a reduced Mo2/Mo3 population is a better discriminator in detecting CMML by flow cytometry. Diagnostic thresholds for these populations have most often been established by comparison with normal donors using PB samples. The objective of this study was to determine the optimal cut-off limits for flow cytometric monocyte repartition (FMR) in bone marrow (BM) samples and to assess the diagnostic accuracy of this method in patients undergoing BM evaluation for other causes of monocytosis using a novel gating strategy. Patients/Methods: 71 BM aspirates were analysed comprising 35 confirmed CMML (21 proliferative CMML , 14 dysplastic CMML), 13 other myeloid neoplasm (MN) disease controls and 23 normal bone marrow aspirates. The MN cohort was identified through local samples with a known peripheral blood monocytosis of ≥1.0x109 and peripheral blast count of <20% not fulfilling criteria for CMML. The MN cohort comprised of 4 cases of chronic myeloid leukaemia (CML), 2 cases of myelodysplasia/myeloproliferative neoplasm unspecified (MDS/MPN-U), two cases of atypical CML (aCML), 2 cases of primary myelofibrosis, 1 case of juvenile myelomonocytic leukaemia (JMML), 1 case of chronic neutrophilic leukaemia (CNL) and 1 case of therapy-related acute myeloid leukaemia (T-AML). The median age of CMML patients within our cohort was 74 years (range 44- 89 years) and 77% were male. Stored flow cytometric data were reanalysed to assess monocyte subsets. After initial removal of doublets and non-cellular events, sequential gating selected a CD45+CD33bright population with low side scatter to select monocytes and exclude granulocytes. The lymphocyte gate as determined on the CD45 vs CD33 plot was used to establish a threshold for CD16 positivity. Immature double negative monocytes (CD14-CD16-) were excluded as in published gating strategies. Results: Linear regression analysis of the percentage of both the Mo1 and Mo2 populations demonstrated a significant difference between the CMML, MN, and normal cohorts (global P<0.0001). Post hoc analysis demonstrated a significant increase in the Mo1% fraction (mean 92.5%; 95% Confidence Interval [CI]: 88.1%, 96.9%) in CMML cases when compared to the normal BM cohort (mean 63.5%; 95% CI: 58.1%, 69.0%; comparison P <0.0001) and the MN cohort (mean 79.9%; 95% CI: 72.7%,87.2%; comparison P = 0.004). Conversely, the Mo2% was significantly decreased (mean 6.8% 95% CI: 2.7%, 10.9%) in CMML cases when compared to the normal cohort (mean 33.0%; 95% CI: 27.9%; 38.1%; comparison P value <0.0001) and the MN cohort (mean 17.6%; 95% CI 10.8%; 24.3%; comparison P value 0.008). Receiver Operator Curve (ROC) curve analysis of the Mo1 population demonstrated an area under the curve (AUC) of 0.8738 (95% CI: 0.7897, 0.9579; P<0.0001) with a marginally lower AUC for Mo2% (Figure 1A). Priority was given to selecting a cut-off value with higher specificity, considering that the BM examination is not a screening test. A cut-off value for Mo1% of >88.5% (Figure 1B) gave a sensitivity of 77.1% (95% CI: 60.98%, 87.93%) and specificity of 88.9% (95% CI: 74.69%, 95.59%) (Figure 1A). A Mo2% cut-off value of <12.25% (Figure 1C) gave a sensitivity of 78.8% (95% CI: 62.25%, 89.32%) with a specificity of 80.6% (95% CI: 64.97%, 90.25%) (Figure 1A). Eight patients with CMML were misclassified by the Mo1/Mo2 criteria. Conclusion: Our study confirms that analysis of monocyte subsets on BM samples provides good discrimination of CMML cases when compared to normal controls. There was significant overlap with other MN samples. FMR may be a useful adjunct to BM morphology in cases with subtle dysplasia. Further study is required to understand the overlap in monocyte phenotype between CMML and other myeloid neoplasms. Disclosures Allen: Amgen: Other: Travel Support; Roche: Other: Travel Support; Novartis: Other: Travel Support. Hahn:Roche: Honoraria; Astra Zeneca: Honoraria.

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THE EMPOWERMENT OF CADRES AND TRADITIONAL BIRTH ATTENDANTS IN THE EARLY DETECTION AND PREVENTION OF STUNTING IN NORTH BOGOR DISTRICT, BOGOR, WEST JAVA

The Indonesian Journal of Public Health ◽

10.20473/ijph.v15i2.2020.153-161 ◽

2020 ◽

Vol 15 (2) ◽

pp. 153

Author(s):

Evi Martha ◽

Nindy Audia Nadira ◽

Trini Sudiarti ◽

Aisyah Putri Mayangsari ◽

Embun Ferdina Enjaini ◽

...

Keyword(s):

Early Detection ◽

Traditional Birth Attendant ◽

Traditional Birth Attendants ◽

Mental Condition ◽

P Value ◽

Screening Methods ◽

Birth Attendants ◽

Increased Risk ◽

Significant Difference ◽

Early Detection And Prevention

Stunting associated to the increased risk of morbidity and mortality, reduced physical capacity, impaired development and function of children’s motor and mental condition. Efforts have been made by the Government through Integrated Healthcare Center (IHC), however it is less optimal as it didn’t involved all aspects of the community. Cadres and TBAs are important parts of the community that are strategic enough to be involved in this activity, because they are very close to mothers and the community.This program aims to improve the knowledge and practice of 31 cadres and traditional birth attendants (TBA) in early detection and prevention of stunting and implement their knowledge to the community by using lecture, discussion, practice, and film screening methods. There is a significant difference in the knowledge level of cadres and TBA between pre and post training (p value = 0.0005) with knowledge improvement of 30.68%. Furthermore, cadre and TBA did a Follow-Up Plan (FUP) in the form of stunting socialization and education, particularly to the expectants and mothers, with the results of about 124 people exposed to stunting. To monitor FUP, team formed a total of 3 Whatsapp Groups for each village as a forum of communication and discussion between fellow participants to PHC and Research Team. As the result, almost all trainees informed the FUP through Whatsapp group, in the form of socialization and education to other cadres and mothers during IHC, recitations, village activities, sports activities, as well as family or neighbors individually, etc. Through the empowerment of cadres and TBA, education on early detection and prevention of stunting goes according to expectations and is quite effective to the role of cadres and TBA in the community.Keywords: stunting, cadre, traditional birth attendant, empowerment

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Serum ferritin, serum LDH and d-dimer in correlation with the outcome in Covid-19.

International Journal of Medical Research and Review ◽

10.17511/ijmrr.2021.i01.07 ◽

2021 ◽

Vol 9 (1) ◽

pp. 46-53

Author(s):

Dr. J. Hari Kishan ◽

◽

Dr. Karthikeya Byalya ◽

Dr. V. Sharvan Kumar ◽

◽

...

Keyword(s):

Platelet Count ◽

Critical Illness ◽

Early Detection ◽

Respiratory Distress ◽

Serum Ferritin ◽

Individual Treatment ◽

P Value ◽

Significant Difference ◽

Wbc Count ◽

D Dimer

BACKGROUND: Early detection of comorbidities, severe patients with SARS-COV-2 and helpfuldiagnostic tools is very important for individual treatment and helps us in assessing the outcome ofmoderate to severe patients. METHOD: In our study we have included 108 patients with RT-PCRpositive for SARS COV-2 and the samples for D-dimer, Sr. LDH and Sr.ferritin were sent andanalyzed. RESULTS: the effect of D-dimer, serum LDH and serum ferritin individually andcombinedly showed in our study there was no significant difference between D-dimer and theoutcome however there was a significant p-value of <0.001 with serum LDH and the outcome, andp-value of 0.016 with serum ferritin and outcome. CONCLUSIONS: When the markers individuallyanalyzed they showed a significant association between outcome and markers, in elevated O2andnormal patients. Mean D dimer, serum ferritin and serum LDH were high among subjects whorequired NIV and low among subjects who required O2, on Room air and intubated respectively. Inhospitalized patients with respiratory distress, we recommend clinicians closely monitor serumferritin, serum LDH and D-dimer and also other markers of severity, WBC count, lymphocyte count,platelet count, IL-6 as markers for potential progression to critical illness, has been under the study,however our study sample being 108, a study with a larger number of samples required to predictthe outcome.

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