scholarly journals Flock level seroprevalence of and risk factors for parainfluenza type 3 virus in small ruminants in northern Jordan

2020 ◽  
Vol 1 (1) ◽  
pp. 22-29
Author(s):  
Waleed Al-Momani ◽  
Mahmoud N. Abo-Shehada

Background. Infectious diseases of the respiratory tract of farm animals are caused by a combination of infectious agents and predisposing factors. Parainfluenza virus type 3 is usually nominated as one of the causes. Seroprevalence and animal risk factors for PI3 infections were investigated in northern Jordan. Methods. The study involved 104 small ruminant flocks (18 sheep, 27 goats, and 59 mixed flocks sampled in northern Jordan. Indirect ELISA was used to test 678 blood samples used in this study. Flocks were identified as infected if at least 1 animal has been detected as positive by the ELISA test. Information regarding production and health management practices was collected in a questionnaire. Statistical analysis was conducted using the Statistical Package for Social Sciences software SPSS 23 (SPSS Inc., Chicago, IL, USA). Results. Flock-level and individual-level seroprevalences were 97%, 37%, and 76%, 11% in sheep and goats respectively. Multivariable logistic regression applied for production and health management practices showed significant risk factors for: Climatic zone (OR= 0.3) was decreasing risk factor for the PI3 seropositivity. Young separated from dams (OR= 4), neonatal deaths (OR= 3), and milking manual (OR= 37.5) were increasing risk factors for the seropositivity of PI3. Conclusions. PI3 virus has a high prevalence in sheep than goats and two risk factors could increase the prevalence of PI3 in both sheep and goats.

2021 ◽  
Vol 2 (1) ◽  
pp. 14-20
Author(s):  
Waleed Al-Momani ◽  
Robin R.A.J. Nicholas ◽  
Mahmoud N. Abo-Shehada

During 2002-2003 the seroprevalence of Mycoplasma capricolum subsp. capricolum was studied among 104 small ruminant’s flocks (18 sheep, 27 goat and 59 mixed flocks) in northern Jordan. At least 5 serum samples/flock were tested using iELISA test. The true flock-level seroprevalences of Mycoplasma capricolum subsp. capricolum were 56%, 39%, 28% in small ruminant (sheep and goats), sheep and goats respectively. There was no significant difference (X2 = 2, d.f. =1, p = 0.15) between seroprevalences in sheep and goats at the flock level. A total of 29 variables including production and health management practices were tested as risk factors for seropositive flocks and analyzed using logistic regression analysis. The use of communal grazing was found to be a risk factor for Mycoplasma capricolum subsp. capricolum seropositivity with odds ratio of 5.2 and drinking the animals with spring water was a protective factor with odds ratio of 0.27. More than half of the examined flocks were seropositive to Mycoplasma capricolum subsp. capricolum indicating a role for Mycoplasma capricolum subsp. capricolum in contagious agalactiae in small ruminants. The education of farmers regarding the use of communal grazing and housing the newborn in separate barns is expected to help reducing the Mycoplasma capricolum subsp. capricolum infections in their flocks.


2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Aziz-ul-Rahman ◽  
Muhammad Abubakar ◽  
Muhammad Hidayat Rasool ◽  
Shumaila Manzoor ◽  
Muhammad Saqalein ◽  
...  

Peste des petits ruminants virus (PPRV) is causing infectious disease with high morbidity and mortality rate in domestic and wild small ruminants of Pakistan with valuable economical losses. The present study was carried out to investigate risk factors of PPRV in domestic small ruminants which were present in the vicinity of wildlife parks. A total of 265 sera samples (27 wild ruminants and 238 domesticated small ruminants) from apparently healthy animals from two different wildlife parks were collected and analysed for PPRV antibodies. Also, 20 nasal swabs from domestic small ruminants showing respiratory signs were collected to check for presence of PPRV antigen. Competitive ELISA revealed highest proportions of anti-PPRV antibodies in domestic small ruminants around the Wildlife Park at Lahore (35%) as compared to Faisalabad (13%), with no existence of PPRV antibodies in tested serum of wild ruminants at these parks. Higher seropositivity was observed in females (25.6%) than in males (5.1%) and in goats (34.5%) compared to sheep (11.2%). The results of N-gene based RT-PCR highlight the absence of PPRV due to lack of current PPR outbreak in the region during study period. Even though grazing was not a significant risk factor, there is still a possibility of wildlife-livestock interactions for feed and water reservoirs, resulting in spillover of PPR to wildlife. Keeping in view the high seropositivity and risk of PPR, vaccination should be adopted to avoid circulation of PPRV among wild and domestic small ruminants (sheep and goats).


2020 ◽  
Author(s):  
Tulsi Ram Gompo ◽  
Rubina Shah ◽  
Ishwari Tiwari ◽  
Yam Bahadur Gurung

Abstract Background Brucellosis is a zoonotic disease of animals and humans caused by the Brucella spps. In Nepal, the presence of brucellosis in small ruminants, e.g., sheep and goats, has impacted the farmers' livelihood and people's food safety. A cross-sectional study was conducted at Rupandehi districts of Nepal to the discover the seroepidemiology and associated risk factors of brucellosis in sheep and goat population. Altogether, 19 sheep and 60 goat farms located in the districts' local units were visited, and the owners were interviewed to get the information on animal characteristics, management, and movement patterns. Three hundred fifty-seven serum samples (80 sheep and 277 goat’s samples) were collected from selected herd based on the probability proportional to their sizes. Each serum sample was tested for Rose Bengal Plate Test (RBPT) and ELISA to estimate the seropositivity. Bivariate analysis followed by multivariable logistic regression was applied to calculate corresponding odds ratios of each variable associated with the brucellosis. Results Out of 80 sheep samples, 12 (15%; 95%CI: 8.79%-24.41%, P<0.001) and out of 277 goat samples 3 (1.1%; 0.37%-3.14%, P<0.001) were tested positive to brucellosis. Age of greater than 1.5 years (OR= 6.39, 95%CI: 1.23, 54.67, p= 0.04) was identified as the significant risk factor for brucellosis in sheep population. While in the goat population, none of the variables were identified as the significant risk factors in multivariable regression analysis. However, the goat from the frequent grazing herds had borderline significance (OR = 8.81, 95%CI: 0.44, 174.56, p<0.15). It might be because of the regular movement of sheep herds that get mixed up with the goat populations. Conclusion The study provides evidence that the burden of brucellosis in sheep is significantly higher than goats. The brucellosis control program in sheep should be applied immediately, as the contiguous herds of sheep and goats keep mixing while grazing and selling. Also, the strict biosecurity and biosafety measures should be implemented among the sheepherders to prevent infection of Brucella in them. We suggest further study on both small ruminants and the sheep owners to reveal the transmission dynamics through one health approach.


2019 ◽  
Vol 147 ◽  
Author(s):  
A. F. A. Pires ◽  
L. Patterson ◽  
E. A. Kukielka ◽  
P. Aminabadi ◽  
N. Navarro-Gonzalez ◽  
...  

Abstract Diversified farms are operations that raise a variety of crops and/or multiple species of livestock, with the goal of utilising the products of one for the growth of the other, thus fostering a sustainable cycle. This type of farming reflects consumers' increasing demand for sustainably produced, naturally raised or pasture-raised animal products that are commonly produced on diversified farms. The specific objectives of this study were to characterise diversified small-scale farms (DSSF) in California, estimate the prevalence of Salmonella enterica and Campylobacter spp. in livestock and poultry, and evaluate the association between farm- and sample-level risk factors and the prevalence of Campylobacter spp. on DSSF in California using a multilevel logistic model. Most participating farms were organic and raised more than one animal species. Overall Salmonella prevalence was 1.19% (95% confidence interval (CI95) 0.6–2), and overall Campylobacter spp. prevalence was 10.8% (CI95 = 9–12.9). Significant risk factors associated with Campylobacter spp. were farm size (odds ratio (OR)10–50 acres: less than 10 acres = 6, CI95 = 2.11–29.8), ownership of swine (OR = 9.3, CI95 = 3.4–38.8) and season (ORSpring: Coastal summer = 3.5, CI95 = 1.1–10.9; ORWinter: Coastal summer = 3.23, CI95 = 1.4–7.4). As the number of DSSF continues to grow, evaluating risk factors and management practices that are unique to these operations will help identify risk mitigation strategies and develop outreach materials to improve the food safety of animal and vegetable products produced on DSSF.


2008 ◽  
Vol 85 (1-2) ◽  
pp. 1-8 ◽  
Author(s):  
Ahmad M. Al-Majali ◽  
Nazmi O. Hussain ◽  
Nadim M. Amarin ◽  
Aggrey A. Majok

2017 ◽  
Vol 38 (4) ◽  
pp. 1875
Author(s):  
José Romero Alexandre Alves ◽  
Clécio Henrique Limeira ◽  
Geilson Manoel de Souza Lima ◽  
Raymundo Rizaldo Pinheiro ◽  
Francisco Selmo Fernandes Alves ◽  
...  

Animal agglomerations at commercial events such as trade fairs represent an important facilitator in infectious disease transmission. Thus, it is crucial to understand the epidemiology of infectious diseases in small ruminants. The objective of this study was to examine lentiviral presence in goats and sheep traded at the animal fair of Tabira city (Sertão region of Pernambuco) and identify possible risk factors associated with infection. We collected serum samples from 233 crossbred goats and 119 crossbred sheep, belonging to 12 breeders, at the Tabira livestock fair from November 2014 to June 2015. An epidemiological questionnaire was used to analyze the risk factors. Agar gel immunodiffusion (AGID) and western blotting (WB) were performed to diagnose small ruminant lentivirus (SRLV) infection. In the AGID test, we used maedi-visna virus (MVV) antigens for sheep and caprine arthritis/encephalitis virus (strain Cork) (CAEV-Co) antigen for goats. The WB analysis used CAEV-Co antigen for both species. Variables from the questionnaire were analyzed with univariate and multivariate statistics. One seropositive goat but no sheep was identified via AGID. According to the WB results, 15/233 goats (6.44%; CI95% = 3.94 - 10.35%) and 8/119 sheep (6.72%; CI95% = 3.45 - 12.71%) were seropositive, totaling 23/352 reactive animals (6.53%; CI95% = 4.39 - 9.61%) from 12 herds. Annual vermifugation of the animals was a significant risk factor (odds ratio = 5.9; CI95% = 1.7-19.8; p = 0.04) for disease in goats, but no variables associated with infection risks were identified in sheep. We concluded that SRLV was present in goats and sheep at the animal fair. Western blots were more sensitive than AGID for SRLV diagnosis. Practices aiming to improve sanitary management may reduce the risk of infection in goats. All studied herds included animals identified as seropositive for SRLV. Therefore, we recommend adopting measures that increase disease diagnosis while intensifying traffic control and surveillance of animal agglomerations.


2020 ◽  
Author(s):  
Po-Han Chen ◽  
Chun-Fang Tung ◽  
Yen-Chung Peng ◽  
Hong-Zen Yeh ◽  
Chi-Sen Chang ◽  
...  

Abstract BackgroundWe investigated whether duodenal major papilla morphology could be a risk factor for failure of selective biliary cannulation (SBC) and post endoscopic retrograde cholangiography and pancreatography (ERCP) complications.MethodsA prospectively recorded database was reviewed retrospectively. Patients were included if they received therapeutic ERCP and had naïve major duodenal papilla. We used Haraldsson’s classification for papilla morphology, as follows: Regular (Type 1), Small (Type 2), Protruding or Pendulous (Type 3) and Creased or Ridged (Type 4). Risk factors for failing SBC and post-ERCP complications were analyzed by multivariate analysis.ResultsA total of 286 cases were included. Age, gender, indications and therapeutic procedures were not different among the four types of papillae. The failure rates of SBC with Type 3 papilla and Type 4 papilla were 11.11% and 6.25%, respectively. In the multivariate analysis, Type 2 papilla (odd ratio 7.18, p= 0.045) and Type 3 papilla (odd ratio 7.44, p= 0.016) were associated with greater SBC failure compared with Type 1 papilla. Malignant obstruction compared to stone (odds ratio 4.45, p=0.014) and age (odd ratio=1.06, p=0.010) were also risk factors for cannulation failure. Type 2 papilla was correlated with a higher rate of post-ERCP pancreatitis (20%, p=0.020) compared to the other types of papilla However, papilla morphology was not a significant risk factor for any complications in the multivariate analysis.ConclusionSmall papilla and protruding or pendulous papilla are more difficult to cannulate compared to regular papilla. Small papilla is associated with a higher rate of post-ERCP pancreatitis.


2009 ◽  
Vol 72 (3) ◽  
pp. 490-496 ◽  
Author(s):  
JOHANNE ELLIS-IVERSEN ◽  
ALASDAIR J. C. COOK ◽  
RICHARD P. SMITH ◽  
GEOFF C. PRITCHARD ◽  
MIRJAM NIELEN

Escherichia coli O157 and Campylobacter jejuni and Campylobacter coli are zoonotic pathogens originating from farm animals. Cattle are the main reservoir for E. coli O157 and also contribute to human cases of campylobacteriosis through contaminated milk, direct contact, and environmental contamination. Thirty groups of young cattle on 30 farms were observed for 7 months and sampled on 4 to 6 separate occasions for E. coli O157 and C. jejuni/coli to characterize shedding patterns and identify risk factors. The within herd prevalence of E. coli O157 per sampling occasion ranged from 0 to 60% (mean = 24%) and average Campylobacter spp. within herd prevalence was 47% ranging from 0 to 100%. The prevalence of E. coli O157–positive herds declined with a linear trend throughout the study from 100 to 38% (OR: 0.5, P &lt; 0.01), whereas time in the study was not significantly associated with Campylobacter prevalence (P = 0.13). Larger herds were more likely to be positive with either or both agents, whereas the number of suckler calves on the farm reduced the risk of both organisms (OR: 0.4/0.6, P &lt; 0.01). Poultry on the premises reduced the risk of E. coli O157, but was not associated with Campylobacter. Emptying and cleaning the water troughs more often than once monthly reduced the risk of detecting Campylobacter and cattle sourced by private water supplies were more likely to be Campylobacter positive. No drinking water management practices were associated with E. coli O157. The risk of detecting both organisms were almost five times higher when the cattle were housed indoors (OR: 4.9, P = 0.03).


Facilities ◽  
2015 ◽  
Vol 33 (7/8) ◽  
pp. 428-453 ◽  
Author(s):  
Ernest Effah Ameyaw ◽  
Albert P.C Chan

Purpose – This paper aims to identify and evaluate the most significant risk factors that strongly affect the implementation of public–private partnership (PPP) water supply projects. PPP for water supply infrastructure services has seen continued growth over the past two decades, following public sector’s budgetary constraints and inability to provide infrastructure-based water services efficiently and cost effectively. However, these projects are often subjected to major risks leading to failures. Design/methodology/approach – Following extensive literature review and case study analyses, an international questionnaire survey was conducted with practicing and experienced PPP experts to establish the significant risks in PPP water projects. Both the probability of occurrence and severity of 40 risks were evaluated by the expert panel to determine their significance and impact on water projects procured under the PPP arrangement. Findings – The paper presents a derived risk factor list, ranks the factors and describes the “top-ranked” risk factors as: poor contract design, water pricing and tariff review uncertainty, political interference, public resistance to PPP, construction time and cost overrun, non-payment of bills, lack of PPP experience, financing risk, faulty demand forecasting, high operational costs and conflict between partners. Originality/value – This factor list broadens PPP stakeholders’ view of important project risks, rather than relying on culture-dependent studies – an area that has received less attention in PPP risk management research. The identified risk factors would provide governments and investors a useful tool in implementing constructive water PPPs by facilitating the development of risk mitigation strategies, particularly for developing countries with poor risk management practices.


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