scholarly journals Editorial: Seminal Transferrin in the Seminal Quality Evaluation of Hemodialytic Patients

2018 ◽  
Vol 2 (5) ◽  
pp. 01-01
Author(s):  
Gilmar Silva
Keyword(s):  
Iron Metabolism ◽  
Sertoli Cells ◽  
Quality Evaluation ◽  
Biological Function ◽  
Seminal Fluid ◽  
Secretory Product ◽  
Iron Ion ◽  
Protective Function ◽  
Ion Concentrations ◽  
Antioxidative Function

The Transferrin is an iron ion transport protein with a biological function that is important to avoid toxic effects due to high intra and extracellular iron ion concentrations [1]. The cellular protective function attributed to transferrin, especially to the germ and support cells (Sertoli cells), is due to its possible antioxidative function exerted together with another protein linked to iron metabolism, ferritin. Seminal transferrin (ST) is an isoform of plasma transferrin, abundant in seminal fluid, secretory product of Sertoli cells (80%)p.

Study on the Antioxidation Activity of Peanut Antioxidant Peptide

2011 ◽  
Vol 140 ◽  
pp. 446-450 ◽  
Author(s):  
Li Na Yu ◽  
Qing Li Yang ◽  
Jie Sun ◽  
Jie Bi ◽  
Chu Shu Zhang ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Free Radical ◽  
Reducing Power ◽  
Peanut Protein ◽  
Raw Material ◽  
Iron Ion ◽  
Antioxidant Peptides ◽  
Antioxidant Peptide ◽  
Antioxidative Function

Use peanut protein powder as the raw material, the five kinds of peanut antioxidant peptides (abbreviated as AP, FP, PP1, NP and PP2, respectively) were obtained through steps of Viscozyme pretreatment and Alcalase, Flavourzyme, Protamex, Neutral protease and Papain hydrolysis, respectively. Four types of antioxidation activities evaluation methods in vitro including scavenging of DPPH free radical, reducing power, iron ion chelation and anti-lipid peroxidation were presented to evaluate the antioxidation activities of peanut antioxidant peptides. The order of antioxidation activities of five antioxidant peptides was PP2>AP>FP>PP1>NP by comprehensive analysis of the antioxidation experimental results. The results indicated that the optimum proteolytic enzyme for preparing antioxidant peptide was papain. Among the five antioxidant peptides, PP2 had the most antioxidation activities of scavenging of DPPH free radical, reducing power, anti-lipid peroxidation. Therefore, the research and development of antioxidant peptide with the antioxidative function by using papain is an effective approach to further exploit peanut protein.

scholarly journals Day and Night GSH and MDA Levels in Healthy Adults and Effects of Different Doses of Melatonin on These Parameters

2011 ◽  
Vol 2011 ◽  
pp. 1-5 ◽  
Author(s):  
Shilpa Chakravarty ◽  
Syed Ibrahim Rizvi
Keyword(s):  
Oxidative Stress ◽  
Membrane Lipid ◽  
Free Radical Scavenger ◽  
Radical Scavenger ◽  
Secretory Product ◽  
Therapeutic Implications ◽  
Antioxidative Function ◽  
Reliable Marker

The pineal secretory product melatonin (chemically, N-acetyl-5-methoxytryptamine) acts as an effective antioxidant and free-radical scavenger and plays an important role in several physiological functions such as sleep induction, immunomodulation, cardiovascular protection, thermoregulation, neuroprotection, tumor-suppression and oncostasis. Membrane lipid-peroxidation in terms of malondialdehyde (MDA) and intracellular glutathione (GSH) is considered to be a reliable marker of oxidative stress. The present work was undertaken to study the modulating effect of melatonin on MDA and GSH in human erythrocytes during day and night. Our observation shows the modulation of these two biomarkers by melatonin, and this may have important therapeutic implications.In vitrodose-dependent effect of melatonin also showed variation during day and night. We explain our observations on the basis of melatonin's antioxidative function and its effect on the fluidity of plasma membrane of red blood cells. Rhythmic modulation of MDA and GSH contents emphasized the role of melatonin as an antioxidant and its function against oxidative stress.

Role of ergothioneine on S-nitrosoglutathione catabolism

2001 ◽  
Vol 356 (3) ◽  
pp. 799-804 ◽  
Author(s):  
Francesco MISITI ◽  
Massimo CASTAGNOLA ◽  
Cecilia ZUPPI ◽  
Bruno GIARDINA ◽  
Irene MESSANA
Keyword(s):  
Biological Function ◽  
Seminal Fluid ◽  
Molecular Event ◽  
Anaerobic Conditions ◽  
High Concentration ◽  
End Products ◽  
Induced Decomposition ◽  
Aerobic And Anaerobic ◽  
Aerobic And Anaerobic Conditions

Ergothioneine (ESH) is a low-molecular-mass thiol present in millimolar concentrations in a limited number of tissues, including erythrocytes, kidney, seminal fluid and liver; however, its biological function is still unclear. In the present study we investigated the role of ESH in the catabolism of S-nitrosoglutathione (GSNO). The results show that: (1) GSNO decomposition is strongly influenced by ESH (k′′ = 0.178±0.032M−1·s−1); (2) ammonia is the main nitrogen-containing compound generated by the reaction; and (3) nitrite is practically absent under both aerobic and anaerobic conditions. These findings are markedly different from those reported for the GSH-induced decomposition of GSNO, in which the nitrogen-containing end products are nitrite, ammonia and nitrous oxide (N2O) under aerobic conditions but nitrite, ammonia, nitric oxide (NO) and small quantities of hydroxylamine under anaerobic conditions. Considering the high concentration of ESH in specific cells, the reaction with GSNO should be considered as an important molecular event occurring in the cell.

scholarly journals Oxidative degradation of rat mast-cell heparin proteoglycan

1990 ◽  
Vol 272 (1) ◽  
pp. 51-57 ◽  
Author(s):  
D D Metcalfe ◽  
H L Thompson ◽  
S J Klebanoff ◽  
W R Henderson
Keyword(s):  
Mast Cell ◽  
Anticoagulant Activity ◽  
Oxidative Degradation ◽  
Human Neutrophils ◽  
Fenton’S Reagent ◽  
Iron Ion ◽  
Fenton's Reagent ◽  
Ion Concentrations ◽  
Normal Human

The susceptibility of rat mast-cell heparin to oxidative degradation was examined. Heparin as a component of intact mast-cell granules (MCG) was degraded following ingestion by normal human neutrophils. In contrast, neutrophils from patients with chronic granulomatous disease (CGD), which do not respond to stimulation with respiratory-burst activity, exhibited a greatly diminished ability to degrade phagocytosed MCG heparin. MCG-associated heparin also was cleaved by H2O2 plus Fe2+ (Fenton's reagent). Isolated heparin proteoglycan (average Mr approx. 750,000) was rapidly cleaved to smaller molecules similar in size to commercial pig heparin upon exposure to Fenton's reagent. This cleavage was inhibited by catalase and by the hydroxyl-radical (OH.)-scavenger mannitol, but not by superoxide dismutase (SOD). The cleavage products retained approx. 26% of the anticoagulant activity of the native molecule. The heparin proteoglycan was also cleaved by acetaldehyde/xanthine oxidase/FeSO4, a system that generates superoxide (O2.-), H2O2 and OH.. Whereas the cleavage at relatively high iron ion concentrations was inhibited by catalase and mannitol but not by SOD, at lower iron ion concentrations the cleavage was inhibited by catalase, mannitol and SOD. These findings suggest the involvement of OH., which at high Fe2+ concentrations is generated by Fenton's reagent (H2O2 plus Fe2+), and at low iron ion concentrations is generated by the iron-ion-catalysed interaction between O2.- and H2O2 (Haber-Weiss reaction). These studies suggest that oxygen radicals generated by activated phagocytes may contribute to the degradation in vivo of both solubilized and granule-associated proteoglycan heparin.

scholarly journals Iron detection and remediation with a functionalized porous polymer applied to environmental water samples

2019 ◽  
Vol 10 (27) ◽  
pp. 6651-6660 ◽  
Author(s):  
Sumin Lee ◽  
Adam Uliana ◽  
Mercedes K. Taylor ◽  
Khetpakorn Chakarawet ◽  
Siva Rama Satyam Bandaru ◽  
...  
Keyword(s):  
Water Samples ◽  
Direct Determination ◽  
Environmental Water ◽  
Porous Polymer ◽  
Environmental Water Samples ◽  
Iron Ion ◽  
Ion Concentrations

Simple and direct determination of iron ion concentrations in water samples is achieved.

scholarly journals Regulation of the Intracellular Free Iron Pool by Dpr Provides Oxygen Tolerance to Streptococcus mutans

2004 ◽  
Vol 186 (18) ◽  
pp. 5997-6002 ◽  
Author(s):  
Yuji Yamamoto ◽  
Kôichi Fukui ◽  
Naoko Koujin ◽  
Hiroaki Ohya ◽  
Kazuhiko Kimura ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
Iron Status ◽  
Iron Concentration ◽  
Iron Ion ◽  
Intracellular Iron ◽  
Free Iron ◽  
Ion Concentrations ◽  
Oxygen Tolerance ◽  
Before And After ◽  
Iron Pool

ABSTRACT Dpr is an iron-binding protein required for oxygen tolerance in Streptococcus mutans. We previously proposed that Dpr could confer oxygen tolerance to the bacterium by sequestering intracellular free iron ions that catalyze generation of highly toxic radicals (Y. Yamamoto, M. Higuchi, L. B. Poole, and Y. Kamio, J. Bacteriol. 182:3740-3747, 2000; Y. Yamamoto, L. B. Poole, R. R. Hantgan, and Y. Kamio, J. Bacteriol. 184:2931-2939, 2002). Here, we examined the intracellular free iron status of wild-type (WT) and dpr mutant strains of S. mutans, before and after exposure to air, by using electron spin resonance spectrometry. Under anaerobic conditions, free iron ion concentrations of WT and dpr strains were 225.9 ± 2.6 and 333.0 ± 61.3 μM, respectively. Exposure of WT cells to air for 1 h induced Dpr expression and reduced intracellular free iron ion concentrations to 22.5 ± 5.3 μM; under these conditions, dpr mutant cells maintained intracellular iron concentration at 230.3 ± 28.8 μM. A decrease in cell viability and genomic DNA degradation was observed in the dpr mutant exposed to air. These data indicate that regulation of the intracellular free iron pool by Dpr is required for oxygen tolerance in S. mutans.

scholarly journals Emotional Concept "Ekel" (Disgust) and its Functional Properties in the Modern German Language

2019 ◽  
Vol 21 (2) ◽  
pp. 529-539
Author(s):  
Izabella V. Korogodina
Keyword(s):  
Functional Properties ◽  
Mental Representation ◽  
Biological Function ◽  
German Language ◽  
Protective Function ◽  
Personal Attitude ◽  
Subjective Assessments ◽  
The Social ◽  
Linguistic Representations

The present research reveals functional properties of emotional concept "Ekel" (disgust) and its linguistic representations in the modern German language. The emotion "disgust" can be defined as a basic emotion that introduces negative personal attitude to the fragments of reality. The mental representation of this emotion is an emotional concept with negative subjective assessments in its structure. The paper describes linguistic representations of disgust, defines its denomination, and outlines the paradigmatic relations of the lexeme Ekel and its linguistic contexts. The author believes that the emotion behind the lexeme "Ekel" fulfils three functions: biological, social, and protective. The biological function is aimed at removal of the object of disgust. The social function lies in the negative characterization of events and the actions of agents. The protective function is in the rejection of potentially dangerous fragments of reality.

scholarly journals Decreased Iron Ion Concentrations in the Peripheral Blood Correlate with Coronary Atherosclerosis

Nutrients ◽  
2022 ◽  
Vol 14 (2) ◽  
pp. 319
Author(s):  
Heyu Meng ◽  
Yueying Wang ◽  
Jianjun Ruan ◽  
Yanqiu Chen ◽  
Xue Wang ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Peripheral Blood ◽  
Coronary Atherosclerosis ◽  
The Body ◽  
Control Group ◽  
Iron Ions ◽  
Iron Ion ◽  
Ion Concentrations ◽  
Obesity And Diabetes

(1) Background: Obesity and diabetes continue to reach epidemic levels in the population with major health impacts that include a significantly increased risk of coronary atherosclerosis. The imbalance of trace elements in the body caused by nutritional factors can lead to the progression of coronary atherosclerosis. (2) Methods: We measured the concentrations of sodium (Na), potassium (K), magnesium (Mg), calcium (Ca), Zinc (Zn), and iron (Fe) in peripheral blood samples from 4243 patients and performed baseline analysis and propensity matching of the patient datasets. The patients were grouped into acute myocardial infarction (AMI, 702 patients) and stable coronary heart disease (SCAD1, 253 patients) groups. Both of these groups were included in the AS that had a total of 1955 patients. The control group consisted of 2288 patients. The plasma concentrations of calcium, magnesium, and iron were measured using a colorimetric method. For comparison, 15 external quality assessment (EQA) samples were selected from the Clinical Laboratory Center of the Ministry of Health of China. SPSS software was used for statistical analysis. The average values and deviations of all of the indicators in each group were calculated, and a p-value threshold of <0.05 was used to indicate statistical significance. (3) Results: The iron ion concentrations of the acute myocardial infarction (AMI) group were significantly lower than the control group (p < 0.05, AUC = 0.724, AUC = 0.702), irrespective of tendency matching. Compared to the data from the stable coronary artery disease (SCAD) group, the concentration of iron ions in the acute myocardial infarction group was significantly lower (p < 0.05, AUC = 0.710, AUC = 0.682). Furthermore, the iron ion concentrations in the (AMI + SCAD) group were significantly lower (p < 0.05) than in the control group. (4) Conclusions: The data presented in this study strongly indicate that the concentration of iron ions in the peripheral blood is related to coronary atherosclerosis. Decreases in the levels of iron ions in the peripheral blood can be used as a predictive biomarker of coronary atherosclerosis.

scholarly journals Locally produced relaxin may affect testis and vas deferens function in rats

Reproduction ◽  
2010 ◽  
Vol 139 (1) ◽  
pp. 185-196 ◽  
Author(s):  
Lais Cavalca Cardoso ◽  
Aline Rosa Nascimento ◽  
Carine Royer ◽  
Catarina Segreti Porto ◽  
Maria Fatima Magalhaes Lazari
Keyword(s):  
Cystic Fibrosis ◽  
Sertoli Cells ◽  
Vas Deferens ◽  
Seminal Fluid ◽  
Fluid Composition ◽  
Mrna Levels ◽  
Ici 182,780 ◽  
Epithelial Secretion ◽  
Tissue Reorganization ◽  
Oxide Synthase

We have previously shown that the rat testis and vas deferens contain high levels of the relaxin receptor, RXFP1. The present study was undertaken to determine the expression of relaxin in these tissues, and the effect of exogenous relaxin on Sertoli cell proliferation and on the mRNA levels of some proteins that may contribute to epithelial secretion and tissue reorganization in the vas deferens. Relaxin mRNA levels in testis and vas deferens were much lower than in the prostate. Sertoli cells seem to be an important source of relaxin mRNA in testis. Relaxin immunoreactivity was detected in the seminiferous epithelium but not in the interstitial compartment. The relaxin precursor was expressed in the vas deferens, and relaxin immunoreactivity was detected in apical cells of the vas deferens. Castration, but not treatment with the anti-estrogen ICI 182,780, dramatically reduced relaxin mRNA levels in the prostate and vas deferens, and this effect was prevented by testosterone.Rxfp1mRNA levels in the vas deferens and prostate were not affected by castration or treatment with ICI 182,780. Exogenous relaxin increased the incorporation of3H-thymidine in cultured Sertoli cells, and treatment of the vas deferens with 100 ng/ml relaxin increased the mRNA levels for the cystic fibrosis chloride channel (cystic fibrosis transmembrane regulator) about three times, and doubled mRNA levels for the inducible form of nitric oxide synthase and metalloproteinase 7. These results suggest that locally produced relaxin acts as an autocrine or paracrine agent in the testis and vas deferens to affect spermatogenesis and seminal fluid composition.

scholarly journals Cyclic Protein-2, a Secretory Product of Rat Sertoli Cells, is the Proenzyme Form of Cathepsin L

1991 ◽  
Vol 5 (12) ◽  
pp. 1789-1798 ◽  
Author(s):  
Moira Erickson-Lawrence ◽  
Sonya D. Zabludoff ◽  
William W. Wright
Keyword(s):  
Sertoli Cells ◽  
Cathepsin L ◽  
Secretory Product
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