scholarly journals Isolation, Identification, Molecular Characterization and Antibiotic Susceptibility Testing of Uro- Pathogenic E.Coli (UPEC) Isolated From Non-Hospitalized Urinary tract infections (UTI)

2020 ◽  
Vol 2 (4) ◽  
pp. 69-73
Author(s):  
Zahoor Ahmed Badini ◽  
Abdu Rauf ◽  
Mumtaz Ali Sanjrani ◽  
Mohammad Rahim Niazi ◽  
Zia-Ud-Din ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Antibiotic Susceptibility ◽  
Clinical Samples ◽  
Specific Gene ◽  
Virulence Determinants ◽  
Zone Size ◽  
Sensitivity Testing ◽  
Tract Infections

Background of the study: Urinary tract infections (UTIs) caused by Uro-pathogenic E.coli (UPEC) considered as most serious infections with increased mortality and morbidity. The ability of UPEC to encode variety of virulence determinants correlated with high recurrence rates and antibiotic resistance. Purpose of the study: The present study focuses on DNA Extraction from E.coli by rapid PCR method and also characterization of Emboli’s molecules. E coli has the carrying ability of many mobile genes, these mobile genes carry the virulence factors. The present study was designed to detect these virulence genetic factors, using phenotypic method like multiplex PCR and detecting capsule synthesis, invasions toxin’s, Adhesions and side-rophores, The study also focused on a specific gene CHUA having ability in heme iron acquisition system and investigation of various virulence determinants expressed by UPEC and their relationship with antibiotic resistance. Methods: Total 15 clinical samples of UPEC were isolated, identified and screened for antibiotic susceptibility pattern. Kirby Bauer disc diffusion test and micro broth dilution method were used to measure the antibiotic sensitivity testing of UPEC isolates. The susceptibility was tested by measuring the zone size after impregnated with antibiotic discs. Results: The interpretation of zone size was done according to the proposed protocol of Clinical Laboratory and Standard Institute (CLSI). Majority of UPEC isolates (22%) were sensitive to tetracycline followed by Norfloxacin (18%). However, least sensitivity was observed against ampicillin (2%) and no sensitivity was experienced against cephalosporin (0%) and penicillin (0%). Conclusion: It has been concluded that majority of UTI patient were suffering from UPEC. Resistance of UPEC against frontline drugs increasing rapidly. Thereby rational and appropriate use of antibiotics is the only way to save important therapeutic options.

scholarly journals Prevalence of antibiotic resistance and blaIMP-1 gene among Pseudomonas aeruginosa strains isolated from burn and urinary tract infections in Isfahan, central Iran

2017 ◽  
Vol 8 (2) ◽  
Author(s):  
Naeimeh Sadat Hashemi ◽  
Meysam Mojiri ◽  
Parivash Yazdani Kachouyi ◽  
Shiva Eskandari ◽  
Mehrsa Mohammadian ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibiotic Resistance ◽  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Diffusion Method ◽  
Clinical Samples ◽  
Resistance Pattern ◽  
Public Health Issue ◽  
High Prevalence ◽  
Tract Infections

Pseudomonas aeruginosa is one of the most important opportunistic pathogens responsible for various types of hospital infections. High prevalence of antibiotic resistance in P. aeruginosa strains of human clinical samples cause more severe diseases for a longer period of time. The current research was done in order to study the distribution of blaIMP-1 gene among the imipenem-resistant P. aeruginosa strains isolated from burn and urinary tract infections of hospitalized patients. Two-hundred and forty-three P. aeruginosa isolates recovered from the cases of burn and urinary tract infections of inpatients and outpatients were analysis for antibiotic resistance pattern using the disk diffusion method. Then, imipenem-resistant isolates were further analyzed for distribution of blaIMP-1 gene using the PCR. Of 243 P. aeruginosa isolates, 146 strains (60.08%) were taken from outpatients and 97 strains (39.91%) were taken from inpatients. P. aeruginosa isolates harbored the highest levels of resistance against streptomycin (100%), nalidixic acid (100%), aztreonam (100%), cotrimoxazole (95.47%), ciprofloxacin (88.47%), cefotaxime (84.36%) and gentamycin (83.95%). Inpatients had a relatively higher levels of antibiotic resistance. One-hundred and twenty-one out of 126 (96.03%) imipenem-resistant P. aeruginosa isolates harbored the blaIMP-1 gene. Inpatients also had a relatively higher prevalence of blaIMP-1 gene. High prevalence of blaIMP-1 gene and also imipenemresistant P. aeruginosa are important public health issue. Clinical laboratories should consider the detection of the blaIMP-1 gene among the P. aeruginosa isolates of clinical samples.

scholarly journals Comparative Genomics of Escherichia coli Strains Causing Urinary Tract Infections

2011 ◽  
Vol 77 (10) ◽  
pp. 3268-3278 ◽  
Author(s):  
Rebecca Munk Vejborg ◽  
Viktoria Hancock ◽  
Mark A. Schembri ◽  
Per Klemm
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Asymptomatic Bacteriuria ◽  
Genomic Islands ◽  
Comparative Genomic ◽  
Specific Gene ◽  
Virulence Determinants ◽  
Content Type ◽  
Tract Infections

ABSTRACTThe virulence determinants of uropathogenicEscherichia colihave been studied extensively over the years, but relatively little is known about what differentiates isolates causing various types of urinary tract infections. In this study, we compared the genomic profiles of 45 strains from a range of different clinical backgrounds, i.e., urosepsis, pyelonephritis, cystitis, and asymptomatic bacteriuria (ABU), using comparative genomic hybridization analysis. A microarray based on 31 completeE. colisequences was used. It emerged that there is little correlation between the genotypes of the strains and their disease categories but strong correlation between the genotype and the phylogenetic group association. Also, very few genetic differences may exist between isolates causing symptomatic and asymptomatic infections. Only relatively few genes that could potentially differentiate between the individual disease categories were identified. Among these were two genomic islands, namely, pathogenicity island (PAI)-CFT073-serUand PAI-CFT073-pheU, which were significantly more associated with the pyelonephritis and urosepsis isolates than with the ABU and cystitis isolates. These two islands harbor genes encoding virulence factors, such as P fimbriae (pyelonephritis-associated fimbriae) and an important immunomodulatory protein, TcpC. It seems that both urovirulence and growth fitness can be attributed to an assortment of genes rather than to a specific gene set. Taken together, urovirulence and fitness are the results of the interplay of a mixture of factors taken from a rich menu of genes.

scholarly journals Community- and Hospital-Acquired Klebsiella pneumoniae Urinary Tract Infections in Portugal: Virulence and Antibiotic Resistance

2019 ◽  
Vol 7 (5) ◽  
pp. 138 ◽  
Author(s):  
Cátia Caneiras ◽  
Luis Lito ◽  
José Melo-Cristino ◽  
Aida Duarte
Keyword(s):  
Antibiotic Resistance ◽  
Urinary Tract ◽  
Klebsiella Pneumoniae ◽  
Urinary Tract Infections ◽  
Multidrug Resistant ◽  
Hospital Environment ◽  
Virulence Determinants ◽  
Antibiotic Resistance Profile ◽  
Tract Infections ◽  
Hospital Acquired

Klebsiella pneumoniae is a clinically relevant pathogen and a frequent cause of hospital-acquired (HA) and community-acquired (CA) urinary tract infections (UTI). The increased resistance of this pathogen is leading to limited therapeutic options. To investigate the epidemiology, virulence, and antibiotic resistance profile of K. pneumoniae in urinary tract infections, we conducted a multicenter retrospective study for a total of 81 isolates (50 CA-UTI and 31 HA-UTI) in Portugal. The detection and characterization of resistance and virulence determinants were performed by molecular methods (PCR, PCR-based replicon typing, and multilocus sequence typing (MLST)). Out of 50 CA-UTI isolates, six (12.0%) carried β-lactamase enzymes, namely blaTEM-156 (n = 2), blaTEM-24 (n = 1), blaSHV-11 (n = 1), blaSHV-33 (n = 1), and blaCTX-M-15 (n = 1). All HA-UTI were extended-spectrum β-lactamase (ESBL) producers and had a multidrug resistant profile as compared to the CA-UTI isolates, which were mainly resistant to ciprofloxacin, levofloxacin, tigecycline, and fosfomycin. In conclusion, in contrast to community-acquired isolates, there is an overlap between virulence and multidrug resistance for hospital-acquired UTI K. pneumoniae pathogens. The study is the first to report different virulence characteristics for hospital and community K. pneumoniae pathogens, despite the production of β-lactamase and even with the presence of CTX-M-15 ESBL, a successful international ST15 clone, which were identified in both settings. This highlights that a focus on genomic surveillance should remain a priority in the hospital environment.

scholarly journals Improving the Management of Pediatric Upper Urinary Tract Infections at Odorheiu Secuiesc Hospital

2020 ◽  
Vol 93 (2) ◽  
pp. 71-76
Author(s):  
Koncsag-Szász Előd ◽  
Szőcs-Gazdi Uzonka ◽  
Magyari Izabella ◽  
Jancsó Réka ◽  
Szalai Mária ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Antibiotic Susceptibility ◽  
Diagnostic Errors ◽  
Upper Urinary Tract ◽  
Study Group ◽  
Protocol Adherence ◽  
E Coli ◽  
Tract Infections

Abstract The primary goal of our retrospective analysis was to check the correctness of the diagnosis, and to identify the potential diagnostic errors among patients coded with N10 diagnosis in 2009, 2014 and 2019 in the pediatric ward of Odorheiu Secuiesc Hospital; we also assessed protocol-adherence in treatment and prophylaxis, and changes in antibiotic resistance of the isolated E. coli strains. During this 10 year-period significant improvements have been achieved in the treatment of upper urinary tract infections: in 2009 the diagnosis could be disputed in 31 out of 61 cases, while in 2019 this ratio is only 2 out of 31 cases; the number of cases pretreated with antibiotics decreased to a third, contaminated urine samples are rare to occur, the unmotivated antibiotics prophylaxis is essentially nonexistent, and there are no major documentation deficiencies either. In our study we present the antibiotic susceptibility of 50 E. coli strains isolated from the study group, which suggests some favorable changes.

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