scholarly journals Combination treatment of vildagliptin and bay leaf (Syzygium polyanthum) extract increased pancreatic beta cells number but have no effect toward glycated albumin levels in diabetic male Wistar rats (Rattus norvegicus)

2019 ◽  
Vol 3 (2) ◽  
pp. 33
Author(s):  
Meiyati Panambunan ◽  
Wimpie Pangkahila ◽  
Anak Agung Gede Budhiarta
Keyword(s):  
Treatment Group ◽  
Beta Cells ◽  
Combination Treatment ◽  
Rattus Norvegicus ◽  
Pancreatic Beta Cells ◽  
Wistar Rats ◽  
Glycated Albumin ◽  
Field Of View ◽  
Control Group ◽  
Male Wistar Rats

Introduction: Diabetes mellitus is a complex and progressive disease which often lead to several debilitating complications that partly caused by by free radicals which can be overcome with antioxidants. Bay leaves contain essential oils, tannins, flavonoids and terpenoids which have considerable antioxidant properties. Therefore, this study aims to determine the effect combination treatment of vildagliptin and bay leaf (Syzygium polyanthum) extract toward pancreatic beta cells density and glycated albumin levels in diabetic male Wistar rats (Rattus norvegicus). Methods: An experimental posttest only control group study was conducted using 36 albino male rats, aged 2-3 months, weighing 180-200 grams. The rats were divided into 2 groups (n= 18) with control group treated with 1.8mg/200g body weight vildagliptin and 2cc placebo (aquabidest) while the treatment group received 1.8mg/200g BW vildagliptin and 250 mg/200g BW bay leaves extract. All treatment lasted for 21 days. Results: The results showed that pancreatic beta cell counts in the treatment group  was significantly higher than the control group  (109.07 ± 20.47 cells/field of view vs 90.87 ± 13.91 cells/field of view; p<0.01). However, the levels of Glycated albumin between two groups were not significantly different (treatment vs control: 17.33 ± 4.51 vs 20.18 ± 4.57; p=0.068). Conclusion: This study suggested that combination treatment of vildagliptin and bay leaf extract increased pancreatic beta cells but did not reduce glycated albumin levels in diabetic male Wistar rats.

scholarly journals Ekstrak Etanol Daun Jati Belanda (Guazuma ulmifolia Lamk) Memperbaiki Profil Lipid Tikus (Rattus norvegicus) Wistar Jantan Dislipidemia

2020 ◽  
Vol 1 (1) ◽  
pp. 25-30
Author(s):  
Melita Hidajat ◽  
I Gusti Made Aman ◽  
Hendro Sukoco ◽  
Ferbian Milas Siswanto
Keyword(s):  
Treatment Group ◽  
Lipid Profile ◽  
Total Cholesterol ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Control Group ◽  
Wistar Strain ◽  
Before And After ◽  
Male Wistar Rats ◽  
Guazuma Ulmifolia

The purpose of this study was to prove that the administration of Jati (Guazuma ulmifolia Lamk) leaves extract improves the lipid profile of dyslipidemic male Wistar rats. Subjects were 20 rats (Rattus norvegicus), male, Wistar strain, dyslipidemia (total cholesterol ≥ 200 mg dl-1), aged 2 months old, weighing 180-200 grams. The control group (10 rats) were given a placebo of 3 ml aquadest (P0) and the treatment group was given extracts of the Jati (Guazuma ulmifolia Lamk) leaves extract of 25 mg kg-1 BW (P1). Before and after treatment for 14 days, total cholesterol, triglyceride, LDL, and HDL levels were examined. The results showed that in the P0 group there were no changes in total cholesterol, triglyceride, LDL, and HDL levels (p>0.05), whereas the P1 group experienced a decrease in total cholesterol, triglyceride and LDL levels (p<0.05) and an increase in HDL levels (p<0.05). The results of this study indicated that the Jati leaves extract was effective to improve the lipid profile of dyslipidemic rats. It was necessary to compare the effectiveness of Jati leaves extract with synthetic dyslipidemia drugs used in the community such as statin.

scholarly journals Pemberian Ekstrak Daun Gandarusa (Justicia gendarussa Burm. f.) Menurunkan Kadar F2-isoprostan Urin Tikus Wistar (Rattus norvegicus) Jantan yang Diinduksi Latihan Fisik Berlebih

2017 ◽  
Vol 9 (3) ◽  
Author(s):  
DG Ika Akpriyanti ◽  
Wimpie Pangkahila ◽  
IGM Aman
Keyword(s):  
Treatment Group ◽  
Physical Training ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Control Group ◽  
Oxygen Species ◽  
Post Test ◽  
Male Wistar Rats ◽  
The Mean

Abstract: Excessive physical activity increases the consumption of oxygen which can result in increased levels of reactive oxygen species (ROS) causing oxidative stress characterized by the elevation of F2-isoprostane as a byproduct of lipid peroxidation. Willow (Justicia gendarussa Burm. f.) has an antioxidant activity that is able to inhibit and break the chain reaction of free radicals and prevent lipid peroxidation. This study was aimed to prove that willow leaf extract could inhibit the elevation of F2-isoprostane levels in male Wistar rats (Rattus norvegicus) urin induced by excessive physical training. This was a true experimental study using 16 male Wistar rats (Rattus norvegicus), aged 2-3 months, weighing 180-200 g as subjects. The control group (P0) was treated with a placebo and excessive physical training, and the treatment group (P1) was treated with the willow leaf extract of 200mg/rat/day and excessive physical training. Before and after 14 days of treatment, the urine samples were collected and their levels of F2-isoprostane 8-isoPGF2α were examined by using enzyme immunoassay kit (EIA). The results showed that the mean level of F2-isoprostane in the control group (P0) before treatment (pretest) was 5.20±0.483 ng / mL while in the treatment group (P1) was 5.46±0.655 ng / mL (P > 0.05). On the other hand, after treatment for 7 days (post-test), the mean level of F2-isoprostane in the control group (P0) was 5.34±0.476 ng/mL and in the treatment group (P1) was 3.61±0.389 ng/mL (P < 0.01). Conclusion: The extract of willow (Justicia gendarussa Burm. f.) leaf could inhibit the elevation of F2-isoprostane levels in male Wistar rats (Rattus norvegicus) urin induced by excessive physical training.Keywords: willow leaf, F2-isoprostane, excessive physical trainingAbstrak: Aktivitas fisik berlebih akan meningkatkan komsumsi oksigen yang dapat mengakibatkan peningkatan kadar reactive oxygen species (ROS) sehingga menyebabkan stres oksidatif yang ditandai dengan meningkatnya F2-isoprostan sebagai salah satu produk peroksidasi lipid. Tanaman gandarusa (Justicia gendarussa Burm. f.) mengandung antioksidan yang dapat mencegah reaksi peroksidasi lipid. Penelitian ini bertujuan untuk membuktikan bahwa pemberian ekstrak daun gandarusa dapat menurunkan kadar F2-isoprostan urin tikus Wistar (Rattus norvegicus) jantan yang diinduksi latihan fisik berlebih. Jenis penelitian ialah eksperimental dengan subjek 16 ekor tikus (Rattus norvegicus), galur Wistar, sehat, berumur 6 bulan, berat badan 180-200 gr, dengan kadar F2-isoprostan >2ng/mL. Kelompok kontrol (P0) diberikan pelatihan fisik berlebih dan plasebo aquadest 2ml sedangkan kelompok perlakuan (P1) diberikan pelatihan fisik berlebih dan ekstrak daun gandarusa dosis 200mg/ekor/hari. Sebelum dan setelah 14 hari perlakuan, sampel urin ditampung dan diperiksa kadar F2-isoprostan menggunakan 8-isoPGF2α enzyme immunoassay kit (EIA) dari assay design untuk data pretest dan post-test. Hasil penelitian menunjukkan rerata kadar F2-isoprostan pada kelompok P0 sebelum perlakuan (pretest) ialah 5,20±0,483 ng/mL, sedangkan pada kelompokP1 ialah 5,46±0,655 ng/mL (P > 0,05). Setelah perlakuan selama 14 hari (post-test), rerata kadar F2-isoprostan pada kelompok P0 ialah 5,34±0,476 ng/mL dan pada kelompok P1 ialah 3,61±0,389 ng/mL (P < 0,01). Simpulan: Ekstrak daun gandarusa (Justicia gendarussa burm. f.) dapat menurunkan kadar F2-isoprostan urin tikus Wistar (Rattus norvegicus) jantan yang diinduksi latihan fisik berlebih.Kata kunci: daun gandarusa, F2-isoprostan, pelatihan fisik berlebih

scholarly journals Oral administration of Bali Robusta coffee (Coffea canephora) extract prevented the reduction of Leydig cells and testosterone levels in male Wistar rats (Rattus norvegicus) with excessive physical training

2021 ◽  
Vol 4 (1) ◽  
pp. 37-41
Author(s):  
Kandy Rosa Ismalia ◽  
Wimpie Pangkahila ◽  
Ni Putu Sriwidyani
Keyword(s):  
Treatment Group ◽  
Physical Training ◽  
Rattus Norvegicus ◽  
Leydig Cells ◽  
Wistar Rats ◽  
Coffea Canephora ◽  
Control Group ◽  
Testosterone Levels ◽  
Robusta Coffee ◽  
Male Wistar Rats

Background: Excessive exercise cause a deterioration of organ function and structure. Overtraining will cause reduce antioxidant reserve and excess free radical production. In this condition, there will be a decrease in the number of Leydig cells in the testis and followed by a decrease in testosterone level. Bali robusta coffee extract contains active compounds such as alkaloids, saponins, flavonoids, tannins and polyphenols. This study aims to prove whether Bali robusta coffee extract (Coffea canephora) can prevent a decrease in the number of Leydig cells and testosterone levels in male Wistar rats (Rattus norvegicus) with excessive physical training. Methods: Thirty-six rats were randomly divided into 2 groups: the control group (placebo aquabidest 2 ml) and the treatment group (Bali robusta coffee extract of 1 gram/kg BW). Both groups received excessive physical training in the form of swimming until the subjects experienced fatigue for 21-days. Results: The results showed that the mean of Leydig cell number in the treatment group was significantly higher, which was 3.70 ± 0.89 cell/field of view, compared to the control group, which was 2.92 ± 0.65 cell/field of view (p = 0.005). The mean testosterone level in the treatment group was also significantly higher, which was 6.32 ± 0.21 nmol/ml, compared to the control group, which was 1.95 ± 0.32 nmol/ml (p < 0.001).     Conclusion: The administration of Bali robusta coffee extract (Coffea canephora) prevented a decrease in the number of Leydig cells and testosterone levels in male Wistar rats (Rattus norvegicus) with excessive physical training.

scholarly journals Histologi Lumen Dan Endotelium Arteri Dorsal Penis Tikus Wistar (Rattus Novergicus) Yang Diinduksi Pakan Tinggi Lemak

2020 ◽  
Vol 7 (1) ◽  
pp. 73
Author(s):  
I Wayan Rosiana ◽  
I Gede Widhiantara
Keyword(s):  
Treatment Group ◽  
High Fat Diet ◽  
Wistar Rats ◽  
Wistar Rat ◽  
Lumen Diameter ◽  
Control Group ◽  
High Fat ◽  
Arterial Lumen ◽  
Male Wistar Rats ◽  
The Difference

This study aims to look at the histopathological picture of the dorsal arteries of the penis of the hiperlipidemic wistar rats (Rattus novergicus) induction by high-fat diet that seen in terms of lumen diameter and thickness of the arterial endotelium wall. Hyperlipidemia is a risk factor for ateriosclerosis in the penile arteries causing erectile dysfunction in men. This study is an experimental study with a randomized posttest only control goup design. The sample are  10 individuals adult male wistar rats aged 3-4 months with a range of body weight 150-200 grams. Before treatment, adaptation was carried out for 7 days. After that the sample rats in the treatment group were made hyperlidemic by feeding lard for 50 days. Then surgery is performed for histopathological preparations at the posttest. To determine the differences in endotelium thickness and arterial lumen diameter in the two groups, an independent t-test was used. Thick diameter data of the endotelium and dorsal arteries of the penis of the wistar rat between the lower treatment group and the control group. The difference that occurred was statistically significant (p <0.05). So it can be concluded that the provision of high-fat diet (hyperlipidemia) decreases the lumen diameter and endotelium thickness of dorsal arteries penis. Keywords: Dorsal arteries, high-fat diet, Wistar rats

scholarly journals The Effect of Soy Milk on Mounting Latency, Mounting Frequency, and Reproductive Development in Male Wistar Rats (Rattus Norvegicus)

2021 ◽  
Vol 9 (B) ◽  
pp. 670-678
Author(s):  
Nurdiana Nurdiana ◽  
Pradnyawati Chania ◽  
Rifzi Nurvitasari ◽  
Azmiatun Nisa ◽  
Styan Wahyu Diana ◽  
...  
Keyword(s):  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Reproductive Development ◽  
Normal Diet ◽  
Male Rats ◽  
Male Rat ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Soy Milk ◽  
Male Wistar Rats

AIM: This research aims to examine the effects of soy milk on mounting latency (ML), mounting frequency (MF), estrogen levels, androgen-binding protein (ABP) expression, and spermatogenesis in male rats (Rattus norvegicus). METHODS: Twenty-four male wistar rats (Rattus norvegicus) aged 4 weeks were divided into four groups. Control group (given a normal diet), P1; P2; P3 (given the normal diet and soy milk powder at doses of 7.1; 14.2; 21.3 g/KgBW/day, respectively) for 6 weeks. Observation of ML and MF were performed at 9 weeks 5 days of age, and rat surgery was performed at 10 weeks of age. Analysis of estrogen hormone levels was conducted by enzyme-linked immunosorbent assay (ELISA), ABP staining was using immunohistochemistry method, testicular spermatogenesis was observed using histopathological methods, and observation of spermatozoa was performed under the microscope.  RESULTS: The results showed no significant reduction of ML and MF, estrogen levels, and ABP expression (p ≤ 0.256; 0.865; 0.959, respectively) in male rat, but there was a significant decrease in the number, morphology, motility of spermatozoa, and testicular histophatology, (p ≤ 0.000, 0.003, 0.008, 0.000, respectively). CONCLUSION: The administrassion of soy milk in various doses (7.1;14.2;21.3 g/KgBW/day) in male Wistar rats (Rattus norvegicus) had showed significantly difference on histopathological evaluation using Johnson’s scoring system, sperm quantity and quality, while on mounting latency and frequency, estrogen levels, and ABP expressions did not show significantly difference between groups. That describe of isoflavone in soy milk can affect several aspects related to male endocrine and reproductive development.

scholarly journals PENGARUH JAMU DENGAN Tribulus terrestris TERHADAP KUALITAS SPERMA TIKUS WISTAR JANTAN (Rattus norvegicus)

2015 ◽  
Vol 3 (2) ◽  
Author(s):  
Delano Pelealu ◽  
Lydia Tendean ◽  
Benny Wantouw
Keyword(s):  
Experimental Study ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Sperm Morphology ◽  
Sperm Quality ◽  
Control Group ◽  
Tribulus Terrestris ◽  
Normal Morphology ◽  
Normal Sperm ◽  
Male Wistar Rats

Abstract: It is known that Tribulus terrestris can improve sperm quality. One of the Indonesian traditional medicine, jamu, contains this Tribulus terrestris. This study aimed to investigate the effect of jamu containing Tribulus terrestris on sperm quality which consisted of concentration, motility, and morphology of spermatozoa of male wistar rats (Rattus norvegicus). This was an experimental study. Nine male wistar rats were randomly divided into 3 groups of 3 rats each: Group P0 (control), group P1 (given 5 mg of Tribulus terrestris), and group P2 (given 10 mg of Tribulus terrestris). This study was conducted for 52 days. The results showed that the spermatozoa concentration of P1 was 49x106 sperm/ml suspension and of P2 53x106 sperm/ml suspension; both were lower than of P0 (59x106 sperm/ml suspension). The sperm motility category (A+B) of P1 was 33% and of P2 (37%); both were lower than of P0 (45%). The motility category A was not found in P1 and P2. The percentage of spermatozoa with normal morphology of P1 was 45% and the abnormal ones was 55%, meanwhile in P2 there was 49% of normal sperm morphology and 51% of abnormal morphology. Conclusion: Jamu containing Tribulus terrestris had no effect in improvement of the sperm quality.Keywords: jamu, Tribulus terrestris, sperm qualityAbstrak: Tribulus terrestris dikenal sebagai bahan yang dapat memperbaiki kualitas sperma. Salah satu jenis jamu yang diproduksi di Indonesia mengandung Tribulus terrestris Penelitian ini bertujuan untuk mengetahui pengaruh jamu dengan Tribulus terrestris terhadap konsentrasi, motilitas, dan morfologi spermatozoa tikus wistar jantan (Rattus norvegicus). Penelitian ini menggunakan metode eksperimental. Sampel 9 ekor tikus wistar jantan (Rattus norvegicus) dibagi menjadi 3 kelompok yakni, kelompok P0 (kontrol); kelompok P1 (pemberian Tribulus terrestris 5 mg); dan kelompok P2 (pemberian Tribulus terrestris 10 mg). Penelitian dilakukan selama 52 hari. Hasil penelitian menunjukkan bahwa konsentrasi spermatozoa P1 49x106 sperma/ml suspensi dan P2 53x106 sperma/ml suspensi lebih rendah dibandingkan P0 59x106 sperma/ml suspensi. Motilitas spermatozoa kategori A+B pada P1 (33%) dan P2 (37%) lebih rendah dibandingkan pada P0 (45%). Tidak ditemukan motilitas kategori A pada P1 dan P2. Morfologi spermatozoa normal pada P1 sebesar 45% dan abnormal 55%. P2 memiliki morfologi spermatozoa normal sebesar 49% dan abnormal 51%. Simpulan: Pemberian jamu dengan Tribulus terrestris tidak meningkatkan kualitas sperma yang mencakup konsentrasi, motilitas, dan morfologi spermatozoa.Kata kunci: jamu, Tribulus terrestris, kualitas sperma

scholarly journals Combination treatment of metformin and red fruit (Pandanus conoideus l.) extract increased pancreatic β cell density but had no effect toward fasting blood glucose and glycated albumin levels in diabetic male wistar rats (Rattus norvegicus)

2019 ◽  
Vol 3 (2) ◽  
pp. 38
Author(s):  
Ingrid Rotinsulu ◽  
Wimpie I. Pangkahila ◽  
A.A. Gede Budhiarta
Keyword(s):  
Blood Glucose ◽  
Treatment Group ◽  
Wistar Rats ◽  
Glycated Albumin ◽  
Fasting Blood Glucose ◽  
Albumin Level ◽  
Control Group ◽  
Pancreatic Β Cell ◽  
Β Cell ◽  
Fruit Extract

Background: Diabetes mellitus (DM) is a degenerative disease associated with premature aging characterized by hyperglycemia. Hyperglycemia in people with DM causes oxidative stress and increases Glycated Albumin level, which is the initial precursor to the formation of AGEs. In turn, AGEs will lead to pancreatic β cell damage and apoptosis. Red fruit (Pandanus conoideus L) contains phytochemicals with antioxidant that has the potential to reduce diabetic complications. Therefore, this study aimed to evaluate the effect of metformin and red fruit extract combination toward blood glucose, glycated albumin and pancreatic β cells density in diabetic rats (Rattus norvegicus). Methods: A post-test only control group study was conducted using 36 male Wistar rats as subject. All subjects were induced for type-2 DM with Streptozotocin and Nicotinamide. The diabetic rats were then divided into 2 groups: the positive control group treated with metformin + placebo and the treatment group treated with metformin + red fruit extract. Pancreatic β cell, fasting blood glucose and glycated albumin assessments were performed after 21 days of treatment. Results: The results showed the mean number of pancreatic β cells in the treatment group was higher than the control group (116.11±33.14 vs 83.20±23.94 cells/visual field; p=0.002). But the mean fasting blood glucose in the treatment group was not significantly different compared to the control group (107.67±14.93 vs. 113.67±11.19 mg/dl; p=0.181). The same result also found in the gycated albumin level (Treatment vs control: 6.97±3.63 vs 6.42±4.01 ng/ml; p=0.666). Conclusion: It can be concluded that the administration of red fruit extract increased the density of pancreatic β cell but did not reduce fasting blood glucose and glycated albumin levels in diabetic Wistar rats.

scholarly journals Intradermal injection of umbilical cord mesenchymal stem cell was more effective than platelet rich plasma in increasing amount of collagen and fibroblasts in Wistar rats (Rattus norvegicus) back skin exposed to ultraviolet B rays

2021 ◽  
Vol 4 (1) ◽  
pp. 42-46
Author(s):  
Anne Carolina Kencanawati ◽  
Wimpie Pangkahila ◽  
Anak Agung Gde Putra Wiraguna
Keyword(s):  
Treatment Group ◽  
Umbilical Cord ◽  
Wistar Rats ◽  
Platelet Rich Plasma ◽  
Intradermal Injection ◽  
Ultraviolet B ◽  
Field Of View ◽  
Control Group ◽  
Skin Collagen ◽  
The Mean

Background: Ultraviolet B exposure is one of the causes of extrinsic skin aging. Ultraviolet exposure may lead to skin collagen damage. This study about mesenchymal stem cells from umbilical cord (UCMSC) compared with platelet rich plasma (PRP) with the aim was to prove that the intradermal injection of UCMSC was more effective than PRP in increasing amount of collagen and fibroblasts in Wistar rats' back skin exposed to Ultraviolet B rays. Methods: This study used post-test only control group design. Subjects were 36 Wistar rats aged 2.5 months. A control group consisting of 18 Wistar rats treated with intradermal injection of PRP and the treatment group with UCMSC. Both groups were exposed to UVB with a total radiation dose of 840 mJ/cm2. The parameter of collagen was determined by picro sirius red staining while fibroblasts by Haematoxylin-Eosin staining. Result: The mean amount of collagen in the control group was lower (57,82±6.52%) than the treatment group (65.69±4.51%) with p<0.001. The result showed there were differences in the number of collagen in both groups (p<0.05). The mean number of fibroblasts in the control group was lower (22.63±5.98 cell/field of view) than the treatment group (28.20±8.90 cell/field of view) with p=0.034. The result showed there were differences in mean of fibroblasts in both groups (p<0.05). Conclusion: It can be concluded that the intradermal injection of UCMSC was more effective in increasing the amount of collagen and fibroblasts than intradermal injection of PRP in Wistar rats' back skin exposed to UVB rays.

scholarly journals THE EFFECT OF SEMBUNG LEAF EXTRACT (BLUMEA BALSAMIFERA) ON THE NUMBER AND DIAMETER OF RATS LEYDIG CELLS INDUCED BY HIGH-FAT DIET

2021 ◽  
Vol 21 (no 1) ◽  
Author(s):  
Gede Widhiantara ◽  
Anak Agung Ayu Putri Permatasari ◽  
Putu Angga Wiradana
Keyword(s):  
Treatment Group ◽  
Leydig Cells ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Control Group ◽  
Control Group Design ◽  
Core Diameter ◽  
Post Test ◽  
Male Wistar Rats ◽  
The Mean

This study aims to determine the morphology of Leydig cells in hyperlipidemic wistar rats after having administered with sembung (Blumea balsamifera) extract orally. This study utilised a randomized post-test only control group design. The sample in this study were 16 adult male wistar rats (Rattus novergicus) aged 3-4 months with a body weight of 150-200 grams, which were equally and randomly divided into two groups, namely control group (hyperlipidaemia and sterile aquadest) and treatment group (hyperlipidaemia and sembung leaf extract). Hyperlipidaemia was induced with lard administration for 50 days. Data were analysed statistically using the Independent t-test. The results showed that the number of Leydig cells in the treatment group was higher than the control group with 68.13 ± 1.89 and 55.63 ± 1.92 cells respectively (P <0.05). In addition, the mean Leydig cell core diameter of the control group, 5.00 ± 0.34 µm, was smaller compared to the treatment group which was 5.80 ± 0.20 µm (P <0.05). It can be inferred that sembung leaf extract provides a protective effect against damage to Leydig cells due to hyperlipidaemia.

ASTAXANTHIN MENGHAMBAT PERLEMAKAN HATI DAN PENINGKATAN KADAR SERUM GAMMA-GLUTAMYLTRANSFERASE PADA TIKUS WISTAR JANTAN YANG DIBERI MINYAK JELANTAH

2020 ◽  
Vol 3 (1) ◽  
pp. 46-55
Author(s):  
Astrid Amanda Pangalela ◽  
I Wayan Weta ◽  
Iin Indrayani Maker
Keyword(s):  
Treatment Group ◽  
Fatty Liver ◽  
Wistar Rats ◽  
Liver Steatosis ◽  
Serum Levels ◽  
Control Group ◽  
Gamma Glutamyltransferase ◽  
Cooking Oil ◽  
Used Cooking Oil ◽  
Male Wistar Rats

ABSTRACT Non-alcoholic fatty liver can be triggered by used cooking oil consumption due to the formation of free radicals and the accumulation of fatty acids in the body. Astaxanthin is a powerful antioxidant that may be able to inhibit the pathogenesis of fatty liver. This study aims to determine the effect of astaxanthin in inhibiting fatty liver (steatosis) and levels of Gamma-Glutamyltransferase (GGT) in male Wistar rats given used cooking oil. An experimental study with Post-test Only Control Group Design was conducted on 36 male Wistar rats aged 3.5-4 months with an approximate bodyweight of 200-210 grams divided randomly into 2 groups. The control group was given 0.42 ml of used cooking oil + 0.5 ml of distilled water, and the treatment group was given 0.42 ml of used cooking oil + 0.2 mg of astaxanthin each day for 14 days. On day 15, blood tests and hepatic histopathology were performed to check  GGT serum levels and steatosis. The comparative test was conducted to compare the results of the control and treatment groups. The results showed that the mean steatosis and GGT levels in the treatment group were significantly lower than the control group. It can be concluded that giving astaxanthin can inhibit fatty liver (steatosis) and increase GGT serum levels in male Wistar rats given used cooking oil.   Keywords: Astaxanthin, Fatty liver, Gamma-glutamyltransferase, Used cooking oil

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