Effect of accelerated solvent extraction conditions on the isolation of bioactive compounds from fennel (Foeniculum vulgare Mill.) seeds

This study focused to establish the optimal conditions of accelerated solvent extraction (ASE), i.e., temperature, static extraction time and number of extraction cycles, for the isolation of fennel (Foeniculum vulgare Mill.) seeds phenols and pigments using 96% ethanol as an extraction solvent. Hence, extraction conditions of temperature (80 and 110 °C), static extraction time (5 and 10 min) and number of extraction cycle (1, 2, 3 and 4) were varied. Obtained extracts were spectrophotometrically analyzed for the content of total phenols (TP), total chlorophylls (TCHL) and total carotenoids (TCAR). Applied extraction conditions had a significant (p<0.01) influence on the yields of analyzed compounds, except for static extraction time on TCAR (p=0.11). Based on the results of statistical analysis, the highest levels of TP and TCHL were achieved at the most invasive conditions (110 °C/10 min/4 cycles), while shorter period was sufficient to reach the highest TCAR yield (110 °C/5 min/3 cycles). In summary, ASE demonstrated to be effective extraction technique for the isolation of fennel seeds hydrophilic and lipophilic bioactive compounds.

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Effect of Particle Size on the Oil Yield and Catechin Compound Using Accelerated Solvent Extraction

Jurnal Teknologi ◽

10.11113/jt.v60.1436 ◽

2013 ◽

Vol 60 (1) ◽

Author(s):

Mohd Azizi Che Yunus ◽

Manzurudin Hasan ◽

Norasikin Othman ◽

Siti Hamidah Mohd-Setapar ◽

Liza Md.-Salleh ◽

...

Keyword(s):

Particle Size ◽

Solvent Extraction ◽

Accelerated Solvent Extraction ◽

Extraction Time ◽

Oil Yield ◽

Particle Sizes ◽

Experimental Range ◽

Areca Catechu ◽

Effect Of Particle Size ◽

Extraction Cycle

Kajian ini bertujuan untuk mengkaji kesan saiz zarah ke atas pengekstrakan sebatian catechin daripada biji Areca catechu L. dengan menggunakan Pengekstrakan Pelarut Terpecut (PPT). Saiz zarah biji Areca catechu dipelbagaikan dari 75 μm sehingga 500 μm. Pengekstrakan telah dijalankan padaparameter tetap iaitu suhu (140oC), tekanan (1500 psi), masa (10 minit), isipadu semburan (60%) dan satu kitaran pengekstrakan, masing-masing. Hasil minyak peratusan yang lebih tinggi adalah 300 mg minyak / gram sampel (30.00% pati minyak) ditemui pada 125 μm. Walaubagaimanapun, kandungan catechin dalam pati minyak hanya 0.0375 mg catechin / gram sampel. Saiz zarah yang terbaik dalam julat uji kaji ini telah dikenal pasti pada 500 μm yang memberikan kandungan catechin yang tinggi iaitu 0.0515 mg catechin / gram sampel dari 247.5 mg minyak / gram sampel (24.75% pati minyak). Kata kunci: Saiz zarah; catechin; LC-MS-TOF; pengekstrakan pelarut terpecut The purpose of this work is to investigate the effects of particle size on the extraction of catechincompound from Areca catechu L. seeds by using Accelerated Solvent Extraction (ASE). The particle sizes of Areca catechu L. seeds are varied from 75 µm until 500 µm. The extraction is conducted at fixed parameters which are temperature (140oC), pressure (1500 psi), extraction time (10 minutes), flush volume (60%) and the static cycle is done for 1 extraction cycle respectively. Higher percentage oil yield of 300mg oil/gram of sample (30.00% oil yield) is found at 125 µm. However, the amount of catechin in oil yields is only 0.0375 mg of catechin/gram of sample. The best of particle size within the experimental range has been identified at 500 µm which gives a high content of catechin with 0.0515 mg Catechin/gram of sample from 247.5 mg oil/gram of sample (24.75% oil yield). Keywords: Particle size; catechin; LC-MS-TOF; accelerated solvent extraction

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Accelerated Solvent Extraction of Insecticides from Rice Hulls, Rice Bran, and Polished Rice Grains

Journal of AOAC International ◽

10.5740/jaoacint.16-0119 ◽

2017 ◽

Vol 100 (2) ◽

pp. 566-572 ◽

Cited By ~ 2

Author(s):

Gustavo Mack Teló ◽

Scott Allen Senseman ◽

Enio Marchesan ◽

Edinalvo Rabaioli Camargo ◽

Katherine Carson

Keyword(s):

Solvent Extraction ◽

Cell Size ◽

Rice Bran ◽

Accelerated Solvent Extraction ◽

Extraction Time ◽

Extraction Temperature ◽

Rice Hulls ◽

Extraction Solvent ◽

Rice Grains ◽

Polished Rice

Abstract Analysis of pesticide residues in irrigated rice grains is important for food security. In this study, we analyzed accelerated solvent extraction (ASE) conditions for the extraction of thiamethoxam and chlorantraniliprole insecticides from rice hulls, rice bran, and polished rice grains. Several variables, including extraction solvent, extraction temperature, extraction pressure, cell size, static extraction time, and sample concentration, were investigated. The average recoveries of the three matrixes were between 89.7 and 109.7% at the fortification level of 0.75 mg/kg. The optimum ASE operating conditions were acetonitrile (100%) as extraction solvent, extraction temperature of 75°C for rice hulls and 100°C for rice bran and polished rice grains, extraction cell pressure of 10.3 MPa, 22 mL cell size, and two extraction cycles. The total extraction time was approximately 25 min. The extracted volume was evaporated to dryness and the residues were redissolved in 2 mL acetonitrile after 1 min of vortex-shaking. Thiamethoxam and chlorantraniliprole were analyzed by ultra-HPLC with tandem MS. In conclusion, ASE in rice hulls, rice bran, and polished rice grains offers the possibility of a fast and simple method for obtaining a quantitative extraction of the studied pesticides.

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Study on Diesel Combustion Dust with ASE and HPLC

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.724-725.1258 ◽

2013 ◽

Vol 724-725 ◽

pp. 1258-1263

Author(s):

Shu Jun Liu ◽

Hao Nan Xu ◽

Kun Feng ◽

Ying Wang

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Solvent Extraction ◽

High Performance ◽

Accelerated Solvent Extraction ◽

Extraction Time ◽

Diesel Combustion ◽

Promising Technique ◽

Extraction Solvent ◽

Optimum Extraction

Diesel combustion dust was analyzed by accelerated solvent extraction-high performance liquid chromatography (ASE-HPLC) in this article. The optimum extraction conditions was that N-hexane was the optimum extraction solvent, extraction time was 30min, temperature was 140°C and the pressure was 120Mpa. it could be a promising technique for the confirmation of the diesel combustion dusts.

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Study on Gasoline Combustion Dust with ASE and HPLC

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.750-752.1852 ◽

2013 ◽

Vol 750-752 ◽

pp. 1852-1854

Author(s):

Shu Jun Liu ◽

Kun Feng ◽

Hao Nan Xu ◽

Ying Wang

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Solvent Extraction ◽

Extraction Method ◽

High Performance ◽

Accelerated Solvent Extraction ◽

Extraction Time ◽

Efficient Extraction ◽

Optimum Extraction

The gasoline combustion dusts was analyzed by accelerated solvent extraction-high performance liquid chromatography(ASE-HPLC) in this paper. The optimum extraction conditions were that extraction time was 30min, temperature was 120°C and the pressure was 100Mpa. It is a efficient extraction method for a laboratory to quickly extract gasoline combustion dust samples.

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Extraction of total carotenoids from peach palm fruit (Bactris gasipaes) peel by means of ultrasound application and vegetable oil

DYNA ◽

10.15446/dyna.v86n209.74840 ◽

2019 ◽

Vol 86 (209) ◽

pp. 91-96 ◽

Cited By ~ 1

Author(s):

Jader Martínez Girón ◽

Luis Eduardo Ordoñez Santos ◽

Diana Ximena Rodríguez-Rodríguez

Keyword(s):

Extraction Time ◽

Solvent Ratio ◽

Total Carotenoids ◽

Palm Fruit ◽

Peach Palm ◽

Extraction Solvent ◽

Ultrasonic Assisted ◽

Ultrasound Application ◽

Predicted Values ◽

Experimental Values

Ultrasonic-assisted extraction (UAE) was applied to extract total carotenoids from dried peach palm peel using soy oil as extraction solvent. Optimization of UAE parameters of total carotenoid extraction from dried peel was done using Response Surface Methodology (RSM) and Central Composite Design (CCD). The extraction parameters were temperature, extraction time and solid to solvent ratio. The optimum UAE conditions were 48°C, extraction time of 28 min, and solid to solvent ratio of 0.0037 g/mL, with a predicted carotenoids content of 151.50 mg/100 g dried peel. To validate the optimized model, the experimental values were compared with the predicted values to check the adequacy of the model. Ultrasound extraction was 33.60% higher than maceration technique. This study indicated that UAE should contribute to a green process for valorization of peach palm by-products.

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Accelerated Solvent Extraction as a Green Tool for the Recovery of Polyphenols and Pigments from Wild Nettle Leaves

Processes ◽

10.3390/pr8070803 ◽

2020 ◽

Vol 8 (7) ◽

pp. 803

Author(s):

Maja Repajić ◽

Ena Cegledi ◽

Valentina Kruk ◽

Sandra Pedisić ◽

Fırat Çınar ◽

...

Keyword(s):

Solvent Extraction ◽

Antioxidant Capacity ◽

Accelerated Solvent Extraction ◽

Ultrasound Assisted Extraction ◽

Extraction Solvent ◽

Green Approach ◽

Assisted Extraction ◽

Chlorophylls A And B ◽

Different Temperatures ◽

Ultrasound Assisted

This study aimed to investigate the performance of accelerated solvent extraction (ASE) as a green approach for the recovery of polyphenols and pigments from wild nettle leaves (NL). ASE was operated at different temperatures (20, 50, 80 and 110 °C), static times (5 and 10 min) and cycle numbers (1–4) using ethanol (96%) as an extraction solvent. In order to compare the efficiency of ASE, ultrasound assisted extraction (UAE) at 80 °C for 30 min was performed as a referent. Polyphenol and pigment analyses were carried out by HPLC and antioxidant capacity was assessed by ORAC. Seven polyphenols from subclasses of hydroxycinnamic acids and flavonoids, along with chlorophylls a and b and their derivatives and six carotenoids and their derivatives were identified and quantified. Chlorogenic acid was the most abundant polyphenol and chlorophyll a represented the dominant pigment. ASE conditions at 110 °C/10 min/3 or 4 cycles proved to be the optimal for achieving the highest yields of analyzed compounds. In comparison with UAE, ASE showed better performance in terms of yields and antioxidants recovery, hence delivering extract with 60% higher antioxidant capacity. Finally, the potential of NL as a functional ingredient from natural sources can be successfully accessed by ASE.

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The Content in Bioactive Compounds of Different Brewers' Spent Grain Aqueous Extracts

Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca Food Science and Technology ◽

10.15835/buasvmcn-fst:12356 ◽

2016 ◽

Vol 73 (2) ◽

pp. 143 ◽

Cited By ~ 3

Author(s):

Anca FARCAS ◽

Sonia SOCACI ◽

Maria TOFANA ◽

Elena MUDURA ◽

Liana SALANTA

Keyword(s):

Antioxidant Activity ◽

Bioactive Compounds ◽

Extraction Time ◽

Aqueous Extracts ◽

Food Ingredients ◽

Extraction Solvent ◽

Methanolic Extracts ◽

Water As Solvent ◽

Food And Beverage ◽

Spent Grain

In the last decade by-products of food and beverage processing have attracted much attention due to their functionality and potential as food ingredients. Brewers’ spent grain is the major by-product of the brewing industry representing a valuable source of bioactive ingredients. The aim of this study was to assess the effect of extraction time and temperature on the efficiency of water as solvent for the extraction of bioactive compounds from brewers’ spent grain (BSG). In terms of extraction efficiency, the results from polyphenols, flavonoids and antioxidant activity, showed that the best extraction parameters for aqueous extracts are 90⁰C and 60 minutes. In comparison with the control, the best extraction method generated 87% of the phenolics and 43.46% of the flavonoids obtained by a methanol extraction. The preliminary results for the aqueous extracts showed that water can be used as extraction solvent, but a higher extraction time and temperature are needed in order to have a content in bioactive compounds similar to that of methanolic extracts. The obtained values for polyphenols, flavonoids and antioxidant activity, emphasize the importance and the opportunities of the reuse of this agro-industrial waste.

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Optimization of accelerated solvent extraction of fatty acids from Coix seeds using chemometrics methods

10.1101/496935 ◽

2018 ◽

Author(s):

Xing Liu ◽

Kai Fan ◽

Wei-Guo Song ◽

Zhengwu Wang

Keyword(s):

Fatty Acids ◽

Solvent Extraction ◽

Accelerated Solvent Extraction ◽

Gas Chromatography Mass Spectrometry ◽

Least Squares Regression ◽

Extraction Solvent ◽

Coix Seed ◽

Relationship Of ◽

The Relationship ◽

Temperature Time

This study investigated the optimization of accelerated solvent extraction (ASE) of fatty acids (FAs) from three Coix seeds (small Coix seed, SCS; big Coix seed, BCS; translucent Coix seed, TCS) by chemometrics methods. Partial least-squares regression (PLSR) and backpropagation neural network (BPNN) were applied to build models that reflect the relationship between content of FAs and extraction conditions (temperature, time, and extraction solvent). Genetic algorithms (GAs) and particle swarm optimization (PSO) were utilized to optimize the combination of extraction conditions. The composition of FAs was analysed by gas chromatography-mass spectrometry (GC-MS). The PLSR models could reflect the relationship of FA content in both BCS and SCS and extraction conditions well, while the BPNN model was more suitable for TCS. The optimal extraction conditions for BCS and SCS were obtained by GAs, whereas those of TCS were obtained by PSO. The FA compositions of the three Coix seeds exhibited differences. The results show that ASE combined with chemometrics methods can rapidly and effectively obtain the optimal conditions for the extraction of FAs from Coix seed and there are differences in the extraction conditions and compositions of FAs among different varieties of Coix seed, but all the extraction time is shorter than other extractions methods.

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Effect of Static Extraction Time on Supercritical Fluid Extraction of Bioactive Compounds from Phyllanthus niruri

Journal of Computational and Theoretical Nanoscience ◽

10.1166/jctn.2020.8742 ◽

2020 ◽

Vol 17 (2) ◽

pp. 918-924 ◽

Cited By ~ 1

Author(s):

Norsyamimi Hassim ◽

Masturah Markom ◽

Masli Irwan Rosli ◽

Shuhaida Harun

Keyword(s):

Supercritical Fluid Extraction ◽

Supercritical Fluid ◽

Bioactive Compounds ◽

Total Content ◽

Operating Conditions ◽

Extraction Time ◽

Fluid Extraction ◽

Active Components ◽

Phyllanthus Niruri ◽

Static Extraction

Supercritical fluid extraction (SFE) is an effective method to extract active components from plants. SFE process can be conducted by using a static or/and dynamic extraction. Static extraction is important for the diffusion of solvated analyte to the matrix surface. Therefore, the main objective of this study is to evaluate the static extraction time influence on SFE extraction of Phyllanthus niruri and the main bioactive compounds. The extraction was conducted by using supercritical carbon dioxide (SC-CO2) with 50% ethanol-water as a co-solvent at selected operating conditions (200 bar, 60 °C, 10% co-solvent content and 3 ml/min solvent total flow rate). The static extraction time was investigated in the range of 15 min–75 min by allowing the plant matrix to immerse in a mixture of SC-CO2 and 50% ethanol-water at the operating condition. The quantification of targeted compounds, which were gallic acid (GA), corilagin (CO) and ellagic acid (EA) were analysed by using high performance liquid chromatography (HPLC). The observation on the physical characteristics of the plant matrix was performed by using the scanning electron microscopy (SEM). The best result for total extraction yield was obtained at 60 min of static extraction time, which was 20.7% g/g sample. It also gave a better result for all three targeted compounds in terms of total content percentage (0.35% g GA/g extract, 3.05% g CO/g extract, and 5.17% g EA/g extract). In conclusion, static extraction time was crucial in extracting bioactive compounds in P. niruri by using SFE method and should be considered as the main parameter other than pressure and temperature.

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