Isolasi dan Uji Patogenitas Isolat Fungi Entomopatogen Terhadap Stadium Dewasa Nyamuk Aedes aegypti

This research aims to determine the effectiveness of four types of entomopathogenic fungi isolated from Ae. aegypti mosquitoes from Bandar Lampung to the mortality of adult stages of Ae. aegypti mosquitoes. This research was conducted in October 2018-January 2019 at the Microbiology Laboratory of FMIPA, University of Lampung. This research using factorial completely randomized design with two factor treatment. The first factor is type of isolate (Mucor sp., Penicillium sp., IL3 (unidentified), Aspergillus sp.) and second faktor is dilution (control, 10 (without dilution), 10-1, 10-2, 10-3). Data were analyzed using ANOVA. If there are significant differences, then it is continued by the Duncan Test at the level of 5%. The results showed that the four types of fungi (Mucor sp., Penicillium sp., Aspergillus sp. and IL3) were able to cause mortality of Ae. aegypti mosquitoes with the highest killing power was in Mucor sp. 10 (without dilution) is 43.33%. But the most effective is Mucor sp. 10-3 because the highest dilution has been able to cause mosquito mortality by 30%. Key words: Ae. aegypti, DHF, entomopathogenic fungi, isolate, dilution.

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Isolasi dan Aplikasi Fungi Entomopatogen dari Larva Nyamuk Aedes aegypti L.

JURNAL BIOLOGI PAPUA ◽

10.31957/jbp.833 ◽

2019 ◽

Vol 11 (2) ◽

pp. 87-93

Author(s):

Wuri Artikasari ◽

Emantis Rosa ◽

Bambang Irawan

Keyword(s):

Aedes Aegypti ◽

Entomopathogenic Fungi ◽

Negative Impact ◽

Hemorrhagic Fever ◽

Mosquito Larvae ◽

Chemical Insecticide ◽

Randomized Design ◽

Significant Difference ◽

Completely Randomized Design ◽

Difference Test

DHF (Dengue Hemorrhagic Fever) is a serious problem in Indonesia. DHF disease control has been applied so far, one of which is the use of larvacide temephos (abate). However larvacide is a chemical insecticide that has a negative impact on human health and causes resistance. Therefore in this research, biological control is carried out by utilizing entomopathogenic fungi as a larvacide. The purpose of this study was to determine the effectiveness of entomopathogenic fungi isolated from Ae aegypti larvae. Against the death of Ae. Aegypti mosquito larvae with the moist chamber method. This study used a completely randomized design (CRD) with a factorial pattern and performed two repetitions. Factor A is a type of fungi with 3 levels, namely A1: Aspergillus sp1, A2: Aspergillus sp2, and A3: Syncephalastrum sp. Factor B is a dilution with 7 levels, namely B0: Control, B1: 100 (without dilution), B2: 10-1, B3: 10-2, B4: 10-3, B5: 10-4, B6: 10-5 with every treatment was applied in 2 repetitions. Observations were made 24 hours after treatment for 3 days. Data were analyzed using analysis of variances (anova) and continued with the Least Significant Difference Test (LSD) at 5%. The results indicate that fungi isolates are the most effective in killing Ae. Aegypti mosquito larvae is Aspergillus sp1 and Aspergillus sp2 on the treatment of spores without dilution. Key words: DHF; larvacide; Aedes aegypti; Entomopathogenic Fungi.

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EFEKTIVITAS INSEKTISIDA NABATI DAUN SALAM (SYZYGIUM POLYANTHUM) TERHADAP MORTALITAS NYAMUK AEDES AEGYPTI

VISIKES: Jurnal Kesehatan Masyarakat ◽

10.33633/visikes.v20i2.4814 ◽

2021 ◽

Vol 20 (2) ◽

Author(s):

Ana Windari ◽

Mimatun Nasihah ◽

Nur Lathifah Syakbanah

Keyword(s):

Aedes Aegypti ◽

Hemorrhagic Fever ◽

Negative Effects ◽

Randomized Design ◽

Mosquito Mortality ◽

Quasi Experimental ◽

Completely Randomized Design ◽

The Right ◽

Mortality Percentage ◽

Leaf Vegetable

Dengue Hemorrhagic Fever (DHF) is an acute disease in the form of dengue virus infection which is spread through mosquito bites. The use of synthetic insecticides actually causes negative effects on the environment, so another alternative is needed, namely the use of bay leaf vegetable insecticides. The purpose of this study was to determine the effectiveness of bay leaf insecticide (Syzygium polyanthum) as an insecticide against the mortality of Aedes Aegypti mosquitoes with the liquid electric method. This study used a quasi-experimental (quasi-experimental) design with a completely randomized design (CRD) method where the experiment was conducted on 150 mosquitoes in 5 treatments and 3 replications. The results of the percentage of bay leaf solution (Syzgium polyanthum) which has the largest number of mosquito mortality at a concentration of 80 ml/200 ml of water with a mortality percentage of 60%. The result of probit LC50 analysis of bay leaf solution was 64,315 g/ml. The higher the concentration of the solution given, the greater the number of mosquito mortality. It is necessary to modify the right tools and methods to make a solution of bay leaf (Syzgium polyanthum) so that it is more effectively applied in the community.Keywords: bioinsecticide, bay leaf, liquid electric, mortality, Aedes aegypti

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Uji Insektisida Serai (Cymbopogon nardus) dan Daun Zodia (Evodia Suaveolens) Terhadap Mortalitas Nyamuk (Aedes aegypti)

Jurnal SAINS ALAMI (Known Nature) ◽

10.33474/j.sa.v2i1.3741 ◽

2019 ◽

Vol 2 (1) ◽

Author(s):

Mahmudi Mahmudi ◽

Hari Santoso ◽

Saimul Laili

Keyword(s):

Aedes Aegypti ◽

Chemical Compounds ◽

Control Treatment ◽

Cymbopogon Nardus ◽

Randomized Design ◽

Mosquito Mortality ◽

Natural Insecticides ◽

Completely Randomized Design ◽

The One ◽

One Way Anova

Natural insecticides made from lemongrass plants and zodia which are environmentally friendly insecticides and contribute to mosquito mortality. The purpose of this study was to determine the effect of lemongrass insecticides (Cymbopogon nardus) and leaves of zodiac (Evodia suaveolens) on mosquito mortality (Aedes aegypti), and to determine the treatment of insecticides that were more effective against the mortality of mosquitoes. The research method used the experimental completely randomized design (CRD) with 4 treatments and 5 replications, namely treatment 1 control; treatment of 2 insecticides zodia; treatment of 3 lemongrass insecticides; and treatment of 4 combination lemongrass insecticides and zodia. The results showed that the control treatment did not effect, whereas in the treatment of zodia insecticides, lemongrass and combinations there were influences, presumably this happened because of difference in the chemical compounds of the 2 plants against mosquitoes. The results showed that mosquito mortality was controlled by 0%; mosquito mortality with zodia insecticide of 28%; mosquito mortality with lemongrass insecticide of 16.4%; and mosquito mortality with combination lemongrass insecticides and zodiac of 43.2%. The results of the One Way ANOVA test showed that there were significant differences and tended to experience increased mosquito mortality after being treated. Insecticides that are more effective in causing mosquito mortality are combination insecticides of lemongrass and zodia leaves.Keywords: Vegetable Insecticides, Lemongrass Plants, Zodia Plants, Mosquitoes.ABSTRAKInsektisida alami yang terbuat dari tanaman serai dan zodia merupakan insektisida alami yang ramah lingkungan dan berperan terhadap mortalitas nyamuk. Tujuan dari penelitian ini untuk mengetahui pengaruh insektisida serai (Cymbopogon nardus) dan daun zodia (Evodia suaveolens) terhadap mortalitas nyamuk (Aedes aegypti) dan untuk mengetahui perlakuan insektisida yang lebih efektif terhadap mortalitas nyamuk (Aedes aegypti). Metode penelitian menggunkan eksperimental Rancangan Acak Lengkap (RAL) dengan 4 perlakuan dan 5 ulangan yaitu perlakuan 1 kontrol; perlakuan 2 insektisida zodia; perlakuan 3 insektisida serai; dan perlakuan 4 insektisida kombinasi serai dan zodia. Hasil penelitian menunjukkan perlakuan kontrol tidak ada pengaruh terhadap mortalitas nyamuk, sedangkan pada perlakuan insektisida zodia, serai serta kombinasi terdapat pengaruh, diduga hal tersebut terjadi karena adanya perbedaan kandungan senyawa kimia dari 2 tanaman tersebut terhadap nyamuk. Hasil penelitian menunjukkan mortalitas nyamuk perlakuan kontrol 0%; mortalitas nyamuk dengan insektisida zodia 28%; mortalitas nyamuk dengan insektisida serai 16,4%; dan mortalitas nyamuk dengan insektisida kombinasi serai dan zodia 43,2%. Hasil analisis uji One Way ANOVA menunjukkan terdapat perbedaan yang signifikan dan cenderung mengalami peningkatan mortalitas nyamuk setelah diberi perlakuan. Insektisida yang lebih efektif dalam mengakibatkan mortalitas pada nyamuk yaitu insektisida kombinasi serai dan daun zodia.Kata kunci: Insektisida Nabati, Tanaman Serai, Tanaman Zodia, Nyamuk

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EFEKTIFITAS CAMPURAN RENDAMAN JERAMI (ORYZA SATIVA L) DAN TEMEFOS SEBAGAI ATRAKTAN TERHADAP LETHAL OVITRAP AEDES AEGYPTI L

Jurnal Ekologi Kesehatan ◽

10.22435/jek.v19i2.3060 ◽

2020 ◽

Vol 19 (2) ◽

pp. 112-118

Author(s):

Muhammad Rasyid Ridha ◽

Budi Hairani ◽

Gusti Meliyanie ◽

Wulan Rasna Giri Sembiring ◽

Abdullah Fadilly ◽

...

Keyword(s):

Aedes Aegypti ◽

Dengue Hemorrhagic Fever ◽

Control Strategy ◽

Oryza Sativa L ◽

Larval Mortality ◽

Hemorrhagic Fever ◽

Alternative Control ◽

Randomized Design ◽

Completely Randomized Design ◽

Global Health Problem

ABSTRACT Dengue hemorrhagic fever is a global health problem and can be transmitted through vectors, namely Aedes aegypti. One of the controls can be through lethal ovitrap combined with attractant. This study aims to compare the percentage of trapped eggs, the number of eggs hatched, and larval mortality in the lethal ovitrap that were given two additional types of attractants. The insecticides used were temephos, while the attractants used were 20% straw soaking water, and water that Ae. aegypti have used to lay eggs. The type of research is an experiment with a completely randomized design. The sample used was female mosquito of Ae. aegypti that is full of blood from laboratory colonization. The results showed that straw soaking water was more influential attractant than the former Ae. aegypti colonization water in attracting Ae. aegypti mosquitoes to lay eggs. The highest larval mortality was found in a combination of lethal ovitrap with straw soaking water. Statistically there is an influence of the type of attractant on hatchability and the development of Ae. aegypti into adult mosquitoes. The combination of lethal ovitrap and attractant of straw soaking water can be an alternative control strategy for DHF program managers to reduce the density of Ae. aegypti mosquitoes and minimize transmission of dengue hemorrhagic fever in an area. Keywords: Aedes aegypti, attractant, lethal ovitrap, straw soaking water ABSTRAK Demam berdarah dengue merupakan masalah kesehatan global dan dapat ditularkan melalui vektor yaitu Aedes aegypti. Salah satu pengendaliannya dapat melalui lethal ovitrap yang dipadukan dengan atraktan. Penelitian ini bertujuan untuk membandingkan persentase jumlah telur terperangkap, jumlah telur menetas, dan mortalitas larva pada lethal ovitrap yang diberi tambahan dua jenis atraktan. Insektisida yang digunakan adalah temefos, sedangkan atraktan yang digunakan adalah air rendaman jerami dengan konsentrasi 20%, dan air bekas kolonisasi/telur larva Ae. aegypti. Jenis penelitian ini adalah eksperimen dengan rancangan acak lengkap. Sampel yang digunakan adalah nyamuk Ae. aegypti betina yang kenyang darah hasil kolonisasi di laboratorium. Hasil penelitian menunjukkan bahwa air rendaman jerami merupakan atraktan yang lebih berpengaruh dibandingkan air bekas kolonisasi Ae. aegypti dalam menarik nyamuk Ae. aegypti untuk bertelur. Mortalitas larva tertinggi terdapat pada kombinasi lethal ovitrap dengan air rendaman jerami. Secara statistik ada pengaruh jenis atraktan terhadap daya tetas dan perkembangan Ae. aegypti menjadi nyamuk dewasa. Kombinasi lethal ovitrap dengan atraktan air rendaman jerami dapat menjadi strategi pengendalian alternatif bagi pengelola program DBD untuk mengurangi kepadatan nyamuk Ae. aegypti dan meminimalisasi transmisi penyakit demam berdarah dengue di suatu wilayah. Kata kunci: Aedes aegypti, atraktan, lethal ovitrap, rendaman jerami

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Analisis Efektifitas Tepung Jamur sebagai Larvasida Aedes aegypti

Jurnal Natur Indonesia ◽

10.31258/jnat.14.1.126-130 ◽

2012 ◽

Vol 14 (1) ◽

pp. 126

Author(s):

Yekki Yasmin ◽

Lenni Fitri ◽

Betty Mauliya Bustam

Keyword(s):

Aedes Aegypti ◽

Beauveria Bassiana ◽

Storage Time ◽

Natural Sciences ◽

The Other ◽

Biology Department ◽

Randomized Design ◽

Two Factors ◽

Result Show ◽

Completely Randomized Design

This research is about effectivity analysis of two kinds of fungi i.e. Beauveria bassiana and Metharrizium anisopliae as Aedes aegyptilarvacide, it has been conducted at laboratories Zoology in Biology Department, Mathematics and Natural Sciences Faculty, Syiah KualaUniversity. Aims of this research were estimating maximum time storage of fungi powder as effective larvacide and the number of bacteriacolonies were found in water used for experiment. This research was used Completely Randomized Design (CRD). The experiments consistof two factors i.e kind and saving time of fungi. The result show that the longer the storage time of Beauveria bassiana the more ineffectiveit was as larvacide and the least fungi colonies were found. Metharrizium anisopliae on the other hand, the longer it was kept, the moreeffective it was as larvacide and the more fungi colonies were found.

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Pengaruh Edible Coating dari Kitosan dengan Konsentrasi Berbeda Terhadap Keumamah selama Masa Penyimpanan

Jurnal Ilmiah Mahasiswa Pertanian ◽

10.17969/jimfp.v4i1.6566 ◽

2019 ◽

Vol 4 (1) ◽

pp. 538-546

Author(s):

Sifa' Fauziyani ◽

Fahrizal Fahrizal ◽

Anshar Patria

Keyword(s):

Thiobarbituric Acid ◽

Edible Coating ◽

Plate Count ◽

Tuna Fish ◽

Penicillium Sp ◽

Randomized Design ◽

Completely Randomized Design ◽

The Difference ◽

Primary Substance ◽

Long Storage

Abstrak. Keumamah (ikan kayu) merupakan makanan tradisional khas Aceh yang dibuat dari ikan tuna.Tetapi ikan kayu (keumamah) tersebut tidak dikemas, yang menyebabkan ikan kayu (keumamah) cepat terserangbakteri dan pertumbuhan jamur jenis Penicillium sp. Untuk itu diperlukan alternatif pengawet alami dalampenelitian ini yaitu kitosan yang berasal dari cangkang kulit udang sebagai bahan dasar pembuatan ediblecoating. Tujuan penelitian ini adalah untuk mengetahui konsentrasi kitosan terbaik sebagai edible coating untukmemperpanjang masa simpan keumamah pada suhu ruang. Penelitian ini menggunakan Rancangan AcakLengkap (RAL) Faktorial dengan 2 faktor. Faktor pertama adalah konsentrasi Kitosan (K) yaitu K0 = 0%kontrol, K2 = 1% Kitosan, K2 = 2% kitosan. Faktor kedua adalah lama penimpanan (P) yaitu P1 =4 minggu,P2= 6 minggu, dan P3=8 minggu. Nilai a w yang dihasilkan pada penelitian ini 0,65, kadar air 4,35%, totalmikroba 7,51 cfu ml/gram, TBA 0,25 manoldehida/gram, kadar protein 36,79%, dan organoleptik tekstur 2,48.Perbedaan konsentrasi kitosan pada keumamah berpengaruh sangat nyata terhadap a w , dan lama penyimpananberpengaruh nyata terhadap kadar air, total mikroba, Thiobarbituric Acid (TBA), analisis protein, danorganoleptik deskriptif aroma . Berpengaruh nyata terhadap nilai organoleptik warna. Dan tidak berpengaruhnyata terhadap uji organoletik tekstur serta tidak terdapatnya bakteri E.coli pada keumamah berkonsentrasikitosan. Pada keumamah konsentrasi terbaik di tunjukkan pada konsentrasi 1% dan 2%.Abstract. Keumamah (wooden fish) is Acehnese traditional food made from tuna fish. But, tradisionalkeumamah was unpackaged, this caused easily attacked by pest and growth penicillium sp. Natural preservativeneeded in this study is chitosan shrimps skin shells as primary substance to make edible coating. The objective ofthis study is to obtain the best chitosan concretations as edible coating to extend keumamah shelf life in roomtemperature. This study used completely randomized design consisting of 2 factors. First factor is chitosanconsentration (K) K0=0% control, K1=1% Chitosan, K2=2% Chitosan. Second factor is long storage (P) P1= 4weaks, P2= 6 weaks, P3= 8 weaks. A w produced in this study is 0,65, moisture 4,35, total plate count 7,51, TBA,0,25 manoldehida/gram, Protein 36,79%, and organoleptic test texture 2,48. The difference of chitosanconsentration in keumamah influenced significantly to a w , and long storage influenced to moisture, TotalPlate Count (TPC), and fragrance and texture organoleptic and not influenced to Thiobarbituric Acid (TBA) andE. coli bacteria not found in keumamah coated by edible coating.

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Comparison between Single and Double Injection of Pituitary Gland (PG) on the Breeding Performance of Climbing Perch, Anabas Testudineus (Bloch)

Journal of Bio-Science ◽

10.3329/jbs.v14i0.443 ◽

1970 ◽

Vol 14 ◽

pp. 57-60

Author(s):

Shahid Uddin Mahmood

Keyword(s):

Pituitary Gland ◽

Key Words ◽

Single Injection ◽

Breeding Performance ◽

Female Fish ◽

Anabas Testudineus ◽

Double Injection ◽

Climbing Perch ◽

Randomized Design ◽

Completely Randomized Design

An investigation was carried out to study the effects of single and double injection of given dose of pituitary gland (PG) on the breeding performance viz., ovulation response, fertilization, and hatching of climbing perch, Anabas testudineus was tested. Pituitary gland dose of 1.2 mg/100g was used in this experiment. Twenty female fish was arranged into two treatments: T1 and T2, each with 10 replications. In T1, the entire dose was administered in a single injection and in T2 the same dose was given in two equal injections three hr apart. The experiment was laid in completely randomized design (CRD). It was found that administration of PG in split doses (double injection) had no additional advantages (ovulation 100%, fertilization 81.56% and hatching 72.68%) over the single injection (ovulation 100%, fertilization 83.03% and hatching 75.48%) treatment. Similarly, the ovulation time of the fish did not vary significantly in either single or double injection of PG (single injection 7.12 hr; double injection 7.20 hr). The results demonstrated that splitting of pituitary gland dose (double injection) has no additional advantages on breeding performance of A. testudineus. Therefore, single injection may be preferred over the second injection for breeding of this fish. Key words: Breeding, pituitary gland (PG), fertilization, hatching J. bio-sci. 14: 57-60, 2006

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Nanoparticle of Silver Nitrate (Ag2NO3) and Carbamate (CO2NH3 derivatives) For Vector Control of Aedes aegypti

E3S Web of Conferences ◽

10.1051/e3sconf/201912516005 ◽

2019 ◽

Vol 125 ◽

pp. 16005

Author(s):

Mursid Raharjo ◽

Agus Subagyo ◽

Sulistyani

Keyword(s):

Aedes Aegypti ◽

Vector Control ◽

Toxicity Test ◽

Experimental Approach ◽

Infectious Agent ◽

Laboratory Research ◽

Nano Silver ◽

Randomized Design ◽

New Form ◽

Completely Randomized Design

The climate change has an impact on various lives, including in the vector breeding. The Aedes aegypti resistance as an infectious agent of dengue fever is harmful for life, especially to non-target organisms and the environment. Nano-silver (Ag2NO3), is a new form of engineering in vector control. The aim of this research was the toxicity test of nano-silver (Ag2NO3) and carbamate (CO2NH3 derivatives) on Aedes aegypti larvae. This was a laboratory research (true experiment), used for 3-4 instar stage larvae toxicity test . The number of samples was calculated using an experimental approach, with a completely randomized design. As simply, can formulated as follows, (t-1) (r-1)> 15. The amount of this samples were 14. The toxicity test with carbamate compounds, at concentration 0.01 mg / l as many as 80% larvae were death, whereas on concentrations 0.5 mg / l as many as 92% of the test animals were death. Toxicity test with nano-silver on concentration 0.3 mg / l, all of the test animals (100%) experienced were death. The results of the observations also showed that the instar more active in the low cocentration of Ag2NO3 (0.01 mg/l - 0.1 mg/l). The conclusion are, nano-silver is more effective than carbamate compounds, and there is a potential for resistance.

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The Insecticide Toxicity and Repelling Ability of Jeruk Purut (Citrus hystrix D.C) Leaf Extract on Maize Weevil (Sitophilus zeamais Motsch)

JURNAL AGRONIDA ◽

10.30997/jag.v5i1.1849 ◽

2019 ◽

Vol 5 (1) ◽

Author(s):

Alfia Wulansari ◽

Nur Rochman ◽

Setyono Setyono

Keyword(s):

Mortality Rate ◽

Key Words ◽

Leaf Extract ◽

Insect Pest ◽

Sitophilus Zeamais ◽

Maize Weevil ◽

Insecticide Toxicity ◽

Randomized Design ◽

Completely Randomized Design ◽

Sitophilus Zeamais Motsch

Sitophilus zeamais Motsch. is a primary storage insect pest damaging maize grains. Control of S. zeamais can be done by applying biopesticide. This study was aimed at assessing the ability of Jeruk Purut leaf extract, as biopesticide, to kill and repel Sitophilus zeamais Motsch. The study was conducted at Entomology Laboratory, Seameo Biotrop in March to August 2018. A completely randomized design with three replicates in each rate of Jeruk Purut leaf extract was used. In preliminary pesticide toxicity experiment, five rates of Jeruk Purut leaf extract, namely 0%, 2%, 4%, 6%, and 8% were applied. In preliminary repelling ability experiment, five rates of Jeruk Purut leaf extract, namely 1%, 2%, 4%, 6%, and 8% were applied. In the main pesticide toxicity and repelling ability trial, five rates of Jeruk Purut leaf extract, namely 3%, 6%, 9%, 12%, and 15% were applied. Results showed that in 24 hour after treating (HAT) no significant effects were found. Significant effects of Jeruk Purut leaf extract as insecticide on S. zeamais were found in 48 and 72 HAT. The highest mortality rate (82.2%) of S. zeamais was found in 15% rate of Jeruk Purut leaf extract in 72 HAT. The application of Jeruk Purut leaf extract was not found to give significant effects on repelling S. zeamais in 24, 48, and 72 HAT as indicated in their sharply fluctuating repellent values. The highest repelling ability (95%) was found in the application of 9% Jeruk Purut leaf extract in 24 HAT. It was concluded that Jeruk Purut leaf extract was more potential as insecticide repellent for Sitophilus zeamais Motsch. Key words: biopesticide, repellent, insecticide, maize, primary storage insect pest

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JAMUR ENTOMOPATOGEN ASAL TANAH LEBAK DI SUMATERA SELATAN DAN POTENSINYA SEBAGAI AGENSIA HAYATI WALANG SANGIT (LEPTOCORISA ORATORIUS (F.))

JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA ◽

10.23960/j.hptt.210154-161 ◽

2010 ◽

Vol 10 (2) ◽

pp. 154-161

Author(s):

Effendy TA ◽

Robby Septiadi ◽

Abdullah Salim ◽

Abdul Mazid

Keyword(s):

Beauveria Bassiana ◽

Analysis Of Variance ◽

Entomopathogenic Fungi ◽

Probit Analysis ◽

Biological Agents ◽

Biocontrol Agents ◽

Stink Bug ◽

Randomized Design ◽

Completely Randomized Design ◽

Leptocorisa Oratorius

Entomopathogenic fungi from the lowland soil of South Sumatera and their potential as biocontrol agents of Stink Bug (Leptocorisa oratorius (F.)). The purpose of this research was to explore and examine the potential of soil borne entomopathogenic fungi from South Sumatera as biological agents of Leptocorisa oratorius (F). Soil used for trapping entomopatogenic fungi, was taken from five locations in South Sumatera. Percentage of nymph mortality was analyzed by using analysis of variance and arranged in Completely Randomized Design. LT50 of nymph mortality was determined with Probit Analysis. Entomopathogenic fungi found in this research were Beauveria bassiana and Metarhizium sp. Stink bug mortality due to B. bassiana isolates reached 40-73.3% and Metarhizium sp. reached 56.7-70%. The results showed that Metarhizium isolates were more virulent than B. bassiana isolates. The viability of B. bassiana conidia did not decrease during sub-culturing. Sub-culturing did not influence the mortality of stink bug nymph but it prolonged the LT50 of the stink bug.

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