scholarly journals EFEKTIVITAS INSEKTISIDA NABATI DAUN SALAM (SYZYGIUM POLYANTHUM) TERHADAP MORTALITAS NYAMUK AEDES AEGYPTI

2021 ◽  
Vol 20 (2) ◽  
Author(s):  
Ana Windari ◽  
Mimatun Nasihah ◽  
Nur Lathifah Syakbanah
Keyword(s):  
Aedes Aegypti ◽  
Hemorrhagic Fever ◽  
Negative Effects ◽  
Randomized Design ◽  
Mosquito Mortality ◽  
Quasi Experimental ◽  
Completely Randomized Design ◽  
The Right ◽  
Mortality Percentage ◽  
Leaf Vegetable

Dengue Hemorrhagic Fever (DHF) is an acute disease in the form of dengue virus infection which is spread through mosquito bites. The use of synthetic insecticides actually causes negative effects on the environment, so another alternative is needed, namely the use of bay leaf vegetable insecticides. The purpose of this study was to determine the effectiveness of bay leaf insecticide (Syzygium polyanthum) as an insecticide against the mortality of Aedes Aegypti mosquitoes with the liquid electric method. This study used a quasi-experimental (quasi-experimental) design with a completely randomized design (CRD) method where the experiment was conducted on 150 mosquitoes in 5 treatments and 3 replications. The results of the percentage of bay leaf solution (Syzgium polyanthum) which has the largest number of mosquito mortality at a concentration of 80 ml/200 ml of water with a mortality percentage of 60%. The result of probit LC50 analysis of bay leaf solution was 64,315 g/ml. The higher the concentration of the solution given, the greater the number of mosquito mortality. It is necessary to modify the right tools and methods to make a solution of bay leaf (Syzgium polyanthum) so that it is more effectively applied in the community.Keywords: bioinsecticide, bay leaf, liquid electric, mortality, Aedes aegypti

scholarly journals EFEKTIFITAS CAMPURAN RENDAMAN JERAMI (ORYZA SATIVA L) DAN TEMEFOS SEBAGAI ATRAKTAN TERHADAP LETHAL OVITRAP AEDES AEGYPTI L

2020 ◽  
Vol 19 (2) ◽  
pp. 112-118
Author(s):  
Muhammad Rasyid Ridha ◽  
Budi Hairani ◽  
Gusti Meliyanie ◽  
Wulan Rasna Giri Sembiring ◽  
Abdullah Fadilly ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Dengue Hemorrhagic Fever ◽  
Control Strategy ◽  
Oryza Sativa L ◽  
Larval Mortality ◽  
Hemorrhagic Fever ◽  
Alternative Control ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Global Health Problem

ABSTRACT Dengue hemorrhagic fever is a global health problem and can be transmitted through vectors, namely Aedes aegypti. One of the controls can be through lethal ovitrap combined with attractant. This study aims to compare the percentage of trapped eggs, the number of eggs hatched, and larval mortality in the lethal ovitrap that were given two additional types of attractants. The insecticides used were temephos, while the attractants used were 20% straw soaking water, and water that Ae. aegypti have used to lay eggs. The type of research is an experiment with a completely randomized design. The sample used was female mosquito of Ae. aegypti that is full of blood from laboratory colonization. The results showed that straw soaking water was more influential attractant than the former Ae. aegypti colonization water in attracting Ae. aegypti mosquitoes to lay eggs. The highest larval mortality was found in a combination of lethal ovitrap with straw soaking water. Statistically there is an influence of the type of attractant on hatchability and the development of Ae. aegypti into adult mosquitoes. The combination of lethal ovitrap and attractant of straw soaking water can be an alternative control strategy for DHF program managers to reduce the density of Ae. aegypti mosquitoes and minimize transmission of dengue hemorrhagic fever in an area. Keywords: Aedes aegypti, attractant, lethal ovitrap, straw soaking water   ABSTRAK Demam berdarah dengue merupakan masalah kesehatan global dan dapat ditularkan melalui vektor yaitu Aedes aegypti. Salah satu pengendaliannya dapat melalui lethal ovitrap yang dipadukan dengan atraktan. Penelitian ini bertujuan untuk membandingkan persentase jumlah telur terperangkap, jumlah telur menetas, dan mortalitas larva pada lethal ovitrap yang diberi tambahan dua jenis atraktan. Insektisida yang digunakan adalah temefos, sedangkan atraktan yang digunakan adalah air rendaman jerami dengan konsentrasi 20%, dan air bekas kolonisasi/telur larva Ae. aegypti. Jenis penelitian ini adalah eksperimen dengan rancangan acak lengkap. Sampel yang digunakan adalah nyamuk Ae. aegypti betina yang kenyang darah hasil kolonisasi di laboratorium. Hasil penelitian menunjukkan bahwa air rendaman jerami merupakan atraktan yang lebih berpengaruh dibandingkan air bekas kolonisasi Ae. aegypti dalam menarik nyamuk Ae. aegypti untuk bertelur. Mortalitas larva tertinggi terdapat pada kombinasi lethal ovitrap dengan air rendaman jerami. Secara statistik ada pengaruh jenis atraktan terhadap daya tetas dan perkembangan Ae. aegypti menjadi nyamuk dewasa. Kombinasi lethal ovitrap dengan atraktan air rendaman jerami dapat menjadi strategi pengendalian alternatif bagi pengelola program DBD untuk mengurangi kepadatan nyamuk Ae. aegypti dan meminimalisasi transmisi penyakit demam berdarah dengue di suatu wilayah. Kata kunci: Aedes aegypti, atraktan, lethal ovitrap, rendaman jerami

scholarly journals Isolasi dan Uji Patogenitas Isolat Fungi Entomopatogen Terhadap Stadium Dewasa Nyamuk Aedes aegypti

2019 ◽  
Vol 11 (1) ◽  
pp. 33-41
Author(s):  
Supiyanto Supiyanto ◽  
Emantis Rosa ◽  
Bambang Irawan ◽  
Nismah Nukmal
Keyword(s):  
Aedes Aegypti ◽  
Key Words ◽  
Entomopathogenic Fungi ◽  
Microbiology Laboratory ◽  
Penicillium Sp ◽  
Randomized Design ◽  
Mosquito Mortality ◽  
Completely Randomized Design ◽  
Aspergillus Sp

This research aims to determine the effectiveness of four types of entomopathogenic fungi isolated from Ae. aegypti mosquitoes from Bandar Lampung to the mortality of adult stages of Ae. aegypti mosquitoes. This research was conducted in October 2018-January 2019 at the Microbiology Laboratory of FMIPA, University of Lampung. This research using factorial completely randomized design with two factor treatment. The first factor is type of isolate (Mucor sp., Penicillium sp., IL3 (unidentified), Aspergillus sp.) and second faktor is dilution (control, 10 (without dilution), 10-1, 10-2, 10-3). Data were analyzed using ANOVA. If there are significant differences, then it is continued by the Duncan Test at the level of 5%. The results showed that the four types of fungi (Mucor sp., Penicillium sp., Aspergillus sp. and IL3) were able to cause mortality of Ae. aegypti mosquitoes with the highest killing power was  in Mucor sp. 10 (without dilution) is 43.33%. But the most effective is Mucor sp. 10-3 because the highest dilution has been able to cause mosquito mortality by 30%.  Key words: Ae. aegypti, DHF, entomopathogenic fungi, isolate, dilution. 

scholarly journals Isolasi dan Aplikasi Fungi Entomopatogen dari Larva Nyamuk Aedes aegypti L.

2019 ◽  
Vol 11 (2) ◽  
pp. 87-93
Author(s):  
Wuri Artikasari ◽  
Emantis Rosa ◽  
Bambang Irawan
Keyword(s):  
Aedes Aegypti ◽  
Entomopathogenic Fungi ◽  
Negative Impact ◽  
Hemorrhagic Fever ◽  
Mosquito Larvae ◽  
Chemical Insecticide ◽  
Randomized Design ◽  
Significant Difference ◽  
Completely Randomized Design ◽  
Difference Test

DHF (Dengue Hemorrhagic Fever) is a serious problem in Indonesia. DHF disease control has been applied so far, one of which is the use of larvacide temephos (abate). However larvacide is a chemical insecticide that has a negative impact on human health and causes resistance. Therefore in this research, biological control is carried out by utilizing entomopathogenic fungi as a larvacide. The purpose of this study was to determine the effectiveness of entomopathogenic fungi isolated from Ae aegypti larvae. Against the death of Ae. Aegypti mosquito larvae with the moist chamber method. This study used a completely randomized design (CRD) with a factorial pattern and performed two repetitions. Factor A is a type of fungi with 3 levels, namely A1: Aspergillus sp1, A2: Aspergillus sp2, and A3: Syncephalastrum sp. Factor B is a dilution with 7 levels, namely B0: Control, B1: 100 (without dilution), B2: 10-1, B3: 10-2, B4: 10-3, B5: 10-4, B6: 10-5 with every treatment was applied in 2 repetitions. Observations were made 24 hours after treatment for 3 days. Data were analyzed using analysis of variances (anova) and continued with the Least Significant Difference Test (LSD) at 5%. The results indicate that fungi isolates are the most effective in killing Ae. Aegypti mosquito larvae is Aspergillus sp1 and Aspergillus sp2 on the treatment of spores without dilution. Key words: DHF; larvacide; Aedes aegypti; Entomopathogenic Fungi.

scholarly journals EFEKTIVITAS ABATE DAN EKSTRAK DAUN SIRIH (Piper betle) DALAM MEMATIKAN LARVA Aedes Aegypti L INSTAR III

Masker Medika ◽  
2020 ◽  
Vol 8 (1) ◽  
pp. 226-232
Author(s):  
Triana Oktaviani ◽  
Zairinayati Zairinayati
Keyword(s):  
Aedes Aegypti ◽  
Leaf Extract ◽  
Hemorrhagic Fever ◽  
Piper Betle ◽  
Mosquito Larvae ◽  
Betel Leaf ◽  
Preventive Effort ◽  
Randomized Design ◽  
Natural Insecticides ◽  
Completely Randomized Design

Latar Belakang: Aedes aegypti merupakan nyamuk yang dapat berperan sebagai vektor Demam Berdarah Dengue (DBD). Upaya promotif dan preventif menjadi mutlak dilakukan untuk memutus mata rantai penularan dengan membunuh larva nyamuk dengan larvasida alami menggunakan ekstrak daun sirih (Piper betle) sebagai insektisida alami. Tujuan dari penelitian: ini adalah untuk mengetahui perbedaan abate dan ekstrak daun sirih (Piper betle) dalam mematikan larva Aedes aegypti L instar III. Desain penelitian: ini bersifat eksperimen dengan menggunakan rancangan acak lengkap (RAL) yang terdiri dari 3 perlakuan 1 kontrol dan 8 kali pengulangan percobaan. Hasil Penelitian: dapat diketahui bahwa untuk penggunaan ekstrak daun sirih (Piper betle) dengan konsentrasi 5% dapat mematikan larva sebanyak 8 ekor, sedangkan konsentrasi 10% tidak dapat mematikan larva (0 larva) dan konsentrasi 15% dapat mematikan larva sebanyak 17 (85%) sedangkan untuk penggunaan abate dengan konsentrasi 5%, 10% dan 15% dapat mematikan larva sebanyak 160 larva (100%). Kesimpulan: bahwa abate mempunyai efektifitas lebih tinggi di banding dengan ekstrak daun sirh (Piper betle).     Background: Aedes aegypti is a mosquito that can act as a vector of dengue Hemorrhagic Fever (DHF). Promotove and preventive effort are absolutely necessary to break the chain to transmission by killing mosquito larvae with natural larvacides using betel leaf extract (Piper betle) as a natural insecticides. The purposes of this study: this was to determine the differences in abate and betel leaf extract (Piper batle) in kliing Aedes aegypti instar III larvae. Research methods this is an experimental study using a completely randomized design (CRD) consisting of 3 treatments 1 control and 8 repetitions of the experiment. The results: it can be seen that for the use of betel leaf extract(Piper betle) with a concentration cannot kill larvae in 17 (85%) whilke for the use of abate with a concentration of 5%, 10% and 15% can kill larvae of 160 (100%). Conclusion: that the abate has a higher effectiveness compared to sirih leaf extract.

scholarly journals Effectiveness of Beauveria bassiana Powder as Larvacide to Aedes aegypti Linnaeus, 1762 Larvae

2018 ◽  
Vol 19 (1) ◽  
pp. 45
Author(s):  
Nova Hariani ◽  
Eka Wulandari ◽  
Bodhi Dharma
Keyword(s):  
Experimental Design ◽  
Aedes Aegypti ◽  
Beauveria Bassiana ◽  
Natural Sciences ◽  
Product Concentration ◽  
Percentage Mortality ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Mortality Percentage ◽  
Third Instar Larvae

Study of effectiveness of Beauveria bassiana Powder as Larvacide to Aedes aegypti larvae was conducted on September 2016 to May 2017 in Ecologi and Systematics Animal Laboratory, Faculty of Mathematics and Natural Sciences, University of Mulawarman. This study aimed to determine the effect of Beauveria bassiana powder with use flour rice media on differents concentration as larvacide to mortality of larvae Aedes aegypti. Stages of study include arranged experimental design (Completely Randomized Design) with concentration of 0%, 0.125%, 0.25%. 0.5% and 0.75%, taking sample, rearing third instar larvae of Aedes aegypti and application Beauveria bassiana powder in 48 hours observation. The mortality value of larvae willbe presented on percentage tabel and graph. The result showed, this product is capable of killing larvae for 48 hours with the percentage mortality 0%, 10%, 28%, 70% and 80% in each concentration. Mortality of larvae is influenced by concentration and period of exposure. Increased of product concentration that given and period of exposure can effected increased of mortality percentage. Keywords: mortality, Beauveria bassiana powder, Aedes aegypti larvae

The resistance status of Aedes aegypti larvae to Temephos in Depok, Sleman, Yogyakarta

2021 ◽  
Vol 2 (1) ◽  
Author(s):  
Mia Munawaroh Yuniyanti ◽  
Sitti Rahmah Umniyati ◽  
Ernaningsih
Keyword(s):  
Aedes Aegypti ◽  
Dengue Hemorrhagic Fever ◽  
Experimental Test ◽  
Larval Mortality ◽  
Dengue Infection ◽  
Hemorrhagic Fever ◽  
Long Time ◽  
Quasi Experimental ◽  
Mortality Percentage

There are still many cases of dengue hemorrhagic fever and tend to increaseover time. One strategy to reduce the increase in cases of dengue infection isto eradicate Aedes aegypti as a vector using insecticides. The use of insecticidesfor a long time can cause resistance. The purpose of this study was to determinethe resistance status of Aedes aegypti larvae against temephos in Depok, Sleman.This quasi experimental test was carried out on 3rd instar larvae of aedesaegypti from RW 9 and 10 Minomartani, Depok, Sleman. The treatment groupwas exposed 0.02 ppm temephos for 24 hours. The analysis was presented in theform of percentage of larval mortality and resistance categories based on WHOguidelines. The mortality percentage of Aedes aegypti larvae in RW 9 was 100%,while the mortality from RW 10 was 97%. Aedes aegypti larvae in Depok district,Sleman are still susceptible to temephos at a dose of 0.02 ppm.

scholarly journals Uji Insektisida Serai (Cymbopogon nardus) dan Daun Zodia (Evodia Suaveolens) Terhadap Mortalitas Nyamuk (Aedes aegypti)

2019 ◽  
Vol 2 (1) ◽  
Author(s):  
Mahmudi Mahmudi ◽  
Hari Santoso ◽  
Saimul Laili
Keyword(s):  
Aedes Aegypti ◽  
Chemical Compounds ◽  
Control Treatment ◽  
Cymbopogon Nardus ◽  
Randomized Design ◽  
Mosquito Mortality ◽  
Natural Insecticides ◽  
Completely Randomized Design ◽  
The One ◽  
One Way Anova

Natural insecticides made from lemongrass plants and zodia which are environmentally friendly insecticides and contribute to mosquito mortality. The purpose of this study was to determine the effect of lemongrass insecticides  (Cymbopogon nardus) and leaves of zodiac (Evodia suaveolens) on mosquito mortality (Aedes aegypti), and to determine the treatment of insecticides that were more effective against the mortality of mosquitoes. The research method used the experimental completely randomized design (CRD) with 4 treatments and 5 replications, namely treatment 1 control; treatment of 2 insecticides zodia; treatment of 3 lemongrass insecticides; and treatment of 4 combination lemongrass insecticides and zodia. The results showed that the control treatment did not effect, whereas in the treatment of zodia insecticides, lemongrass and combinations there were influences, presumably this happened because of difference in the chemical compounds of the 2 plants against mosquitoes. The results showed that mosquito mortality was controlled by 0%; mosquito mortality with zodia insecticide of 28%; mosquito mortality with lemongrass insecticide of 16.4%; and mosquito mortality with combination lemongrass insecticides and zodiac of 43.2%. The results of the One Way ANOVA test showed that there were significant differences and tended to experience increased mosquito mortality after being treated. Insecticides that are more effective in causing mosquito mortality are combination insecticides of lemongrass and zodia leaves.Keywords: Vegetable Insecticides, Lemongrass Plants, Zodia Plants, Mosquitoes.ABSTRAKInsektisida alami yang terbuat dari tanaman serai dan zodia merupakan insektisida alami yang ramah lingkungan dan berperan terhadap mortalitas nyamuk. Tujuan dari penelitian ini untuk mengetahui pengaruh insektisida serai (Cymbopogon nardus) dan daun zodia (Evodia suaveolens) terhadap mortalitas nyamuk (Aedes aegypti) dan untuk mengetahui perlakuan insektisida yang lebih efektif terhadap mortalitas nyamuk (Aedes aegypti). Metode penelitian menggunkan eksperimental Rancangan Acak Lengkap (RAL) dengan 4 perlakuan dan 5 ulangan yaitu perlakuan 1 kontrol; perlakuan 2 insektisida zodia; perlakuan 3 insektisida serai; dan perlakuan 4 insektisida kombinasi serai dan zodia. Hasil penelitian menunjukkan perlakuan kontrol tidak ada pengaruh terhadap mortalitas nyamuk, sedangkan pada perlakuan insektisida zodia, serai serta kombinasi terdapat pengaruh, diduga hal tersebut terjadi karena adanya perbedaan kandungan senyawa kimia dari 2 tanaman tersebut terhadap nyamuk. Hasil penelitian menunjukkan mortalitas nyamuk perlakuan kontrol 0%; mortalitas nyamuk dengan insektisida zodia 28%; mortalitas nyamuk dengan insektisida serai 16,4%; dan mortalitas nyamuk dengan insektisida kombinasi serai dan zodia 43,2%. Hasil analisis uji One Way ANOVA menunjukkan terdapat perbedaan yang signifikan dan cenderung mengalami peningkatan mortalitas nyamuk setelah diberi perlakuan. Insektisida yang lebih efektif dalam mengakibatkan mortalitas pada nyamuk yaitu insektisida kombinasi serai dan daun zodia.Kata kunci: Insektisida Nabati, Tanaman Serai, Tanaman Zodia, Nyamuk

scholarly journals Daya Predasi Ikan Lemon (Labidochromis caeruleus) dan Ikan Kapiat (Barbonymus schwanenfeldii) Terhadap Larva Nyamuk Aedes aegypti

2021 ◽  
Vol 13 (1) ◽  
pp. 37-46
Author(s):  
Nathania Disa Ariesta Andriani ◽  
Hebert Adrianto ◽  
Arief Gunawan Darmanto
Keyword(s):  
Aedes Aegypti ◽  
Cichlid Fish ◽  
Hemorrhagic Fever ◽  
Fish Predation ◽  
Randomized Design ◽  
Biological Vector ◽  
Post Test ◽  
Vector Borne ◽  
Completely Randomized Design ◽  
The Difference

Dengue hemorrhagic fever (DHF) is a vector-borne disease that still happens every year in Indonesia. This disease is caused by the dengue virus and transmitted by the Aedes aegypti as its vector. One of the biological vector controls that can be implemented is using fish as a predator of mosquito larvae, such as using Electric Yellow Cichlid fish (Labidochromis caeruleus) and Tinfoil Barb fish (Barbonymus schwanenfeldii). This study aimed to determine the difference in predation capacities of Electric Yellow Cichlid and Tinfoil Barb on Ae. aegypti larvae. This research is quasiexperimental with a completely randomized design (CRD) with Post Test Only research design. One aquarium with three liters of water containing one fish is given 25 Ae. aegypti larvae. Testing of each fish species is carried out in five replications. Fish predation was observed every 30 seconds until all larvae were eaten. The result showed that Electric Yellow Cichlid could prey on 25 larvae in an averagetime of 5.7 minutes, while Tinfoil Barb spent 11.6 minutes. The results of the Friedman test showed that there was a significant time difference in preying time of Electric Yellow Cichlid and Tinfoil Barb. It’s concluded that Electric Yellow Cichlid was more effective as an Ae. aegypti larvae predator.

scholarly journals Analisis Efektifitas Tepung Jamur sebagai Larvasida Aedes aegypti

2012 ◽  
Vol 14 (1) ◽  
pp. 126
Author(s):  
Yekki Yasmin ◽  
Lenni Fitri ◽  
Betty Mauliya Bustam
Keyword(s):  
Aedes Aegypti ◽  
Beauveria Bassiana ◽  
Storage Time ◽  
Natural Sciences ◽  
The Other ◽  
Biology Department ◽  
Randomized Design ◽  
Two Factors ◽  
Result Show ◽  
Completely Randomized Design

This research is about effectivity analysis of two kinds of fungi i.e. Beauveria bassiana and Metharrizium anisopliae as Aedes aegyptilarvacide, it has been conducted at laboratories Zoology in Biology Department, Mathematics and Natural Sciences Faculty, Syiah KualaUniversity. Aims of this research were estimating maximum time storage of fungi powder as effective larvacide and the number of bacteriacolonies were found in water used for experiment. This research was used Completely Randomized Design (CRD). The experiments consistof two factors i.e kind and saving time of fungi. The result show that the longer the storage time of Beauveria bassiana the more ineffectiveit was as larvacide and the least fungi colonies were found. Metharrizium anisopliae on the other hand, the longer it was kept, the moreeffective it was as larvacide and the more fungi colonies were found.

scholarly journals PENGARUH JENIS PELARUT TERHADAP AKTIVITAS ANTIOKSIDAN EKSTRAK MENIRAN (Phyllanthus niruri L.)

2020 ◽  
Vol 9 (4) ◽  
pp. 458
Author(s):  
Rizky Amalia Rachmawati ◽  
Ni Wayan Wisaniyasa ◽  
I Ketut Suter
Keyword(s):  
Antioxidant Activity ◽  
Total Phenol ◽  
Total Phenol Content ◽  
Flavonoid Content ◽  
Phyllanthus Niruri ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Four Levels ◽  
Effect On Yield ◽  
The Right

This study was conducted to determine the effect of different solvents on the antioxidant activity of gale of the wind extract and to find out the right type of solvent used to extract gale of the wind with the highest antioxidant activity using maceration method. The experimental design used in this study was a completely randomized design (CRD) with a type of solvent as a treatment. The treatment consisting of four levels, namely methanol, ethanol, acetone, isopropanol. All treatments were repeated four times so that they were obtained 16 experimental units. The data obtained were analyzed by variance and if the treatment had significant effect followed by Duncan test. The results showed that the type of solvent treatment had a very significant effect on yield, total phenol, total flavonoids, total tannin, and antioxidant activity (IC50). The results showed that acetone solvent had the best treatment which produced a yield of 22.58%, total phenol content of 188.77 mg GAE/g, total flavonoid content of 247.60 mg QE/g, total tannin content of 297.51mg TAE/g, and antioxidant activity (IC50) of 15.19 ppm.

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