Produk Urikase dari Bacillus sp. Kontaminan Laboratorium

Urease is an enzyme catalyst in the reaction of oxidation of uric acid into Allantoin. Oxidation of uric acid by the enzyme has become a basic principle of measurement of uric acid levels in the human body. This research to know urikase derived from the bacterium Bacillus sp that contaminate the air Laboratory of Microbiology Department of Health Analyst Banjarmasin. The research is descriptive survey. Airborne bacteria were isolated and identified for Bacillus sp. The identification is done by examination of macroscopic, microscopic and biochemical tests subsequently performed tests using nutrient agar medium containing 0.2% of uric acid. Results of the study showed there were two isolates of Bacillus sp and obtained clear zone on both these isolates. Conclusions of research there are contaminants Bacillus sp. as much as 40% in the laboratory. 100% of the yield urikase Bacillus sp. Urikase advice from Bacillus sp can be used as an alternative reagent uric acid probes spectophotometric method.

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Optimization of alkali-thermostable and cellulase-free eylanase production from Bacillus Sp.

Journal of Bio-Science ◽

10.3329/jbs.v19i0.12994 ◽

2012 ◽

Vol 19 ◽

pp. 7-14

Author(s):

SCD Sharma ◽

MS Shovon ◽

AKM Asaduzzaman ◽

MG Sarowar Jahan ◽

T Yeasmin ◽

...

Keyword(s):

Wheat Bran ◽

Agar Medium ◽

Xylanase Activity ◽

Effect Of Temperature ◽

Bacillus Sp ◽

Dilution Technique ◽

Isolation And Identification ◽

Xylanase Production ◽

Optimization Of Process Parameters ◽

Bacterium Bacillus

Context: To analyze the nutritional and physicochemical parameters for the production of alkali-thermostable and cellulase free xylanase from bacteria. Objectives: The aim of this study was to isolation and identification and of alkali-thermostable and cellulase free xylanase producing bacteria from soil as well as optimization of process parameters for xylanase production. Materials and Methods: The bacterium Bacillus sp. was isolated from soil by serial dilution technique on xylan agar medium and identified by morphological and biochemical studies. The production of xylanase was carried out on xylan broth medium and xylanase activity was assayed by dinitrosalicylic acid (DNS) method. The effect of cultural parameters on the production of xylanase was determined by measuring the activity of xylanase. The effect of temperature and pH on the activity of partially purified xylanase as well as substrate specificity of xylanase were examined. Results: The maximum xylanase production (4000 U/L) by a Bacillus sp. was attained when the medium containing 0.5% wheat bran xylan and peptone at pH 8.0 and 50-55°C within 48-60 h. The partially purified xylanase was optimally active at pH 9.0 and 55°C. The xylanase showed high substrate activity towards wheat bran xylan but no activity towards cellulose, carboxymethyl cellulose and starch. Thus the enzyme was alkali-thermostable and cellulase free xylanase. Conclusion: The results obtained in this study suggest that the Bacillus sp. used is highly potential and useful for the production of cellulase free xylanase. DOI: http://dx.doi.org/10.3329/jbs.v19i0.12994 J. bio-sci. 19: 7-14, 2011

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Aktivitas Enzim Amilase Isolat Bakteri Amilolitik dari Tepung Sagu Basah dan Lingkungan Tempat Penyediaannya Secara Tradisional di Jayapura

JURNAL BIOLOGI PAPUA ◽

10.31957/jbp.457 ◽

2018 ◽

Vol 6 (2) ◽

pp. 47-52

Author(s):

Suprapto Surapto ◽

Tri Gunaedi ◽

Basa T. Rumahorbo

Keyword(s):

Enzyme Activity ◽

Agar Medium ◽

Amylase Activity ◽

Soluble Starch ◽

Bacterial Isolates ◽

Clear Zone ◽

Plate Method ◽

Crude Enzyme Extract ◽

Nutrient Agar Medium ◽

Bacillus Subtilis Atcc

The study about the activity of the enzyme amylase from amylolytic bacterial isolates from wet sagoo starch and its traditional provision environment had been done in Jayapura. The purposes of this study were to determine the activity of amylase enzyme and to identify the bacteria isolated from wet sagoo starch and its processing environment in Jayapura district. The method used was an experimental laboratorium in which isolation of amylolytic bacteria was performed by using nutrient agar medium with 1% soluble starch on spreed pour plate method. The enzyme activity was detected with 0.2% iodine in 2% potassium iodide which were able to form a clear zone. The protein content of the crude enzyme extract was determined by the Bradford method using bovine serum albumin (BSA). Amylase enzyme activity was determined by the formula: DUN/ml = [(R0-R1)/R0] [dilution factor] DUN/ml (dextrinizing units per ml). The results showed that there were 15 isolates amylolytic bacteria. Four (4) bacterial isolates have amylolytic power of more than 30 mm. The amilase activity of amylolytic bacterial of all isolates were quite high: which were 35 577, 18 903, 32 106 and 46 600 U/mg for SU4, SU13, SU23 and SU40 respectively. The identification of isolates indicated that the three isolates are members of the Bacillus cereus ATCC 14 579 types with a similarity value of 71.70% to 81.10%, and one isolate is Bacillus subtilis ATCC 6501 members with a similarity value of 94.30%. Keywords: Amylolytic bacteria, amylase activity, characterization, sago flour.

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Isolation and screening of resistant bacteria of heavy metal (Fe) at ship dismantling

MATEC Web of Conferences ◽

10.1051/matecconf/201819713020 ◽

2018 ◽

Vol 197 ◽

pp. 13020 ◽

Cited By ~ 1

Author(s):

Harmin Sulistiyaning Titah ◽

Herman Pratikno ◽

Atiek Moesriati ◽

Rizky Islami Putera ◽

Muhammad Fauzul Imron

Keyword(s):

Sea Water ◽

Agar Medium ◽

Iron Concentration ◽

Ferrous Metal ◽

Resistant Bacteria ◽

Qualitative And Quantitative Analysis ◽

Clear Zone ◽

Qualitative And Quantitative ◽

Ferrous Metals ◽

Nutrient Agar Medium

The activity of ship dismantling is one of the sources of metal pollutant that polluted the environment. The aims of this study were analyze the iron concentration from the ship dismantling area, to isolate the bacteria from those area, and to determine the bacteria resistant on iron. Samplings was conducted in three point sampling at sea water and soil coast, respectively. Isolation of bacteria was carried out using pour plate methods. All isolated bacteria in seawater and soil samples were inoculated on nutrient agar medium (NA) containing ferrous metals (Fe2+) with various concentrations (0; 1,000; 2,000; 3,000; 4,000; 5,000; and 6,000 μg/mL). Based on the results, sea water and soil indicated that those area have contaminated with iron. The concentration of iron in seawater was 1.03, 1.01 and 1.00 μg/mL, respectively. Meanwhile, the concentration of iron in soil was 962.0, 966.05, 981.00 mg/kg, respectively. The result of qualitative and quantitative analysis showed that the isolates of AT, AL and CL coded bacteria have high resistance to the effect of iron. It indicated with clear zone of 6.00-7.00 mm at 6,000 μg/mL. In conclusion, both of bacteria are potential to be used for bioremediation of the ferrous metal (Fe2+) in further investigation.

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CELLULOLYTIC BACTERIA MANGROVE LEAF LITTER IN BANGKA ISLAND

Samakia : Jurnal Ilmu Perikanan ◽

10.35316/jsapi.v9i1.218 ◽

2018 ◽

Vol 9 (1) ◽

pp. 06-11

Author(s):

Ardiansyah Kurniawan ◽

Asep Awaludin Prihanto ◽

Suci Puspitasari ◽

Andi Kurniawan ◽

Euis Asriani ◽

...

Keyword(s):

Bacillus Subtilis ◽

Leaf Litter ◽

Agar Medium ◽

Cellulose Degradation ◽

Bacterial Isolates ◽

Cellulolytic Bacteria ◽

Clear Zone ◽

Biochemical Tests ◽

Positive Isolate ◽

Degradation Ability

The study aimed to obtain isolate of cellulolytic bacteria from leaf litter on mangrove in Bangka Island. Sampling was conducted on mangroves in Sungailiat, Bangka and Tukak Sadai, South Bangka district. The isolation was carried out using 1% enriched agarmedia of Carboxymetyl Cellulose (CMC). The bacterial isolates were tested with cellulolytic growth on 1% enriched CMC agar medium and lugol added at 72 hours. The clear zone resultingindicates cellulose degradation ability. The positive isolate of cellulolyticwas identified by biochemical tests. 1 of 5 isolates in Sungailiat positive cellulolytic mangroves and identified as Bacillus subtilis. 2 of 5 isolates in mangrove Tukak Sadai is positive as a cellulolytic bacteria and identified as Staphylococcus saproviticus and Bacillus cereus.

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Production and Characterization of Extracellular Protease from Bacillus sp. 140-B Isolated from Pineapple Plantation in Lampung, Indonesia

KnE Life Sciences ◽

10.18502/kls.v3i4.701 ◽

2017 ◽

Vol 3 (4) ◽

pp. 170

Author(s):

Nur Laili ◽

Sarjiya Antonius

Keyword(s):

Protease Activity ◽

Extracellular Enzymes ◽

Agar Medium ◽

Skim Milk ◽

Bacillus Sp ◽

Enzyme Properties ◽

Clear Zone ◽

Optimum Ph ◽

Effects Of Temperature

Bacillus sp. being industrially important organisms produces a wide variety of extracellular enzymes including protease. Bacillus sp. 140-B isolated from rhizosphere area of pineapple plantation in Lampung Province, Indonesia was tested for production of protease on skim milk agar medium. The aims of this study were to characterize and investigate some properties of protease activity from Bacillus sp. 140-B. Bacillus sp. 140-B showed protease activity qualitatively by clear zone diameter of 15.0 mm and the highest protease activity was 35.02 Unit · mg–1 protein at 14 h after incubation. Some properties of protease activities from Bacillus sp. 140-B including effects of temperature, pH and several metals were observed in this experiment. The protease activity from Bacillus sp. 140-B had optimum pH of 7,0 and the optimum temperature was 60 °C. Several metals which were evaluated on protease activity showed that Mn could increase protease activity, while other metals (Ca, Co, and Hg) showed decreasing protease activity of Bacillus sp. 140-B.<div> Keywords: Bacillus sp. 140-B; enzyme activity; enzyme properties; protease</div>

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Isolation, Screening and Methionine Production by Bacillus species isolated from Poultry Waste

Abasyn Journal Life Sciences ◽

10.34091/ajls.1.2.6 ◽

2018 ◽

pp. 95-101

Keyword(s):

Escherichia Coli ◽

Agar Medium ◽

Bacillus Species ◽

Poultry Waste ◽

Bacillus Sp ◽

Biochemical Tests ◽

Gram Staining ◽

Solid Agar ◽

Hemolysis Test ◽

Oxidase Test

The current study was focused on isolating and identifying methionine producing bacteria from poultry waste. The bacteria were isolated from poultry waste and the ones which specifically produced methionine on solid agar medium, using a methionine requiring auxotroph (Escherichia coli) were kept for further analysis. Methionine assay was also carried out after fermentation to quantify methionine produced by each isolate. A total of 10 bacteria were isolated of which 2 were confirmed as active methionine producers after halo growth was observed on the solid agar medium. These 2 isolates which produced higher amount of methionine were ultimately selected for further investigation. The methionine producing isolates which were designated PW1 and PW3 were identified as Bacillus sp. respectively after biochemical tests were carried out. The test carried out include; gram staining, motility test, catalase test, citrate test, hemolysis test, indole test, penicillin sensitivity, oxidase test and crystal colony formation. Where PW3 (Bacillus sp.) produced greater amount of methionine (0.54 mg/l), while PW1 (Bacillus sp.) produced 0.20 mg/l after the completion of the methionine assay. Keywords: Keywords: Methionine production, Bacillus species, poultry waste.

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The detection of streptococci in air

Epidemiology and Infection ◽

10.1017/s0022172400015254 ◽

1950 ◽

Vol 48 (4) ◽

pp. 504-524 ◽

Cited By ~ 21

Author(s):

R. E. O. Williams ◽

Ann Hirch

Keyword(s):

Crystal Violet ◽

Agar Medium ◽

Horse Serum ◽

Great Majority ◽

The Other ◽

Standard Errors ◽

Airborne Bacteria ◽

Potassium Tellurite ◽

Nutrient Agar Medium ◽

The Mean

To estimate the number of viable streptococci present in the air of occupied places, airborne bacteria were collected in a slit-sampler on a nutrient agar medium containing 5% sucrose, 5% horse serum, 0·25 mg./100 ml. crystal violet, and 1·0/100 ml. potassium tellurite. This medium inhibits the great majority of the staphylococci and micrococci found in the air. On it colonies ofStrep. salivariusare readily recognized by their mucoid form. The number of other streptococci was estimated by picking and examining a random sample of the colonies.Streptococci were recognized in the sample by three different methods: (1) by microscopic examination of a nigrosin film made from the original colony; (2) by examination of a nigrosin film and also subculturing the colony to a ditch plate having blood agar on one side and serum agar containing 40% or bile on the other; or (3) by subculture to bile-agar ditch plates alone, the bile agar in this case containing aesculin and ferric citrate. By method (1) streptococci could be distinguished from micrococci; by methods (2) and (3) this distinction could be made with more confidence, and in addition enterococci could be distinguished from other streptococci.A number of tests of the efficiency of the method were carried out, and it was concluded that some 20–40% of the viable streptococci in the air were missed.The standard errors of the mean of a number of streptococcal counts in schoolrooms were calculated and found to be of the order of 14% of the mean when our standard routine was followed; increasing the size of the sample of colonies picked for examination by 30% would probably have had only a trivial effect on the standard error of the mean, since the greater part of the variation was due to differences between the counts in different rooms.

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