scholarly journals EFEKTIVITAS LARVASIDA ANTARA ABATE, EKSTRAK DAUN SIRSAK (Annona muricata Linn) DAN EKSTRAK DAUN SIRIH (Piper betle Linn) TERHADAP KEMATIAN LARVA Aedes aegypti INSTAR 3 TAHUN 2015

2015 ◽  
Vol 34 (3) ◽  
pp. 175-179
Author(s):  
Kurnia Agung Indra Pastha ◽  
Aris Santjaka
Keyword(s):  
Aedes Aegypti ◽  
Piper Betle ◽  
Annona Muricata ◽  
Group Design

Larvasida kimiayang digunakan untuk pemberantasan vektor DBD berdampak terjadinya resistensi.Larvasida nabati mampu mematikan larva Aedes aegypti pada penelitian sebelumnya.Larvasida nabati yangdigunakan yaitu ekstrak daun sirsak dan ekstrak daun sirih. Tujuan penelitian ini dapat diketahuinya efektifitaslarvasida antara abate, ekstrak daun sirsak (Annona muricata Linn) dan ekstrak daun sirih (Piper betle Linn)terhadap kematian larva A. aegypti.Jenis penelitian ini true experimental dengan desain the randomized posttestonlycontrol group design. Variabel dependent berupa jumlah larva A. aegypti, variabel independent berupaberbagai dosis larvasida. Analisis statistik yang digunakan Anova one way dengan uji lanjut Least SignificantDifference. Hasil penelitian ini rata-rata kematian larva A. aegypti yang tertinggi pada larvasida abate dosis 0,1gr/l yaitu 25 (100%), ekstrak daun sirsak dosis 1 gr/l yaitu 23,3 (93,3%) dan ekstrak daun sirih dosis 1 gr/l yaitu17,6 (70,6%). Hasil analisis statistik terbukti signifikan dengan nilai p=0,003 α (0,05), sehingga ada perbedaankematian larva A. aegypti terhadap jenis larvasida. Hasil penelitian ini dapat disimpulkan larvasida paling efektifabate, larvasida nabati paling efektif ekstrak daun sirsak dibanding ekstrak daun sirih. Abate tetap bisa digunakandalam pemberantasan larva A. aegypti, tetapi jika tidak ada abate bisa dengan ekstrak daun sirsak atau ekstrakdaun sirih.

scholarly journals EFEKTIVITAS DAYA LARVASIDA EKSTRAK DAUN DAN BIJI SIRSAK (Annona muricata L.) TERHADAP MORTALITAS LARVA NYAMUK Aedes aegypti (Studi di Laboratorium Parasitologi)

2019 ◽  
Vol 6 (1) ◽  
pp. 34-38
Author(s):  
Wiji Santi Yuliani ◽  
Awaluddin Susanto ◽  
Hindyah Ike Suhariati
Keyword(s):  
Aedes Aegypti ◽  
Control Group ◽  
Annona Muricata ◽  
Control Group Design ◽  
Purposive Sampling ◽  
Group Design ◽  
Post Test ◽  
Test Control

Pendahuluan:Sirsak (Annona muricata L.) memiliki bagian tanaman sirsak seperti buah, daun, biji, dan batang dapat dimanfaatkan untuk kesehatan karena mengandung antifeedant, insektisida, larvasida, antioksidan, antikanker, dan antivirus. Nyamuk Aedes aegypti  dapat menyebabkan penyakit DBD (Demam Berdarah Dengue). Dalam pemeriksaan ini bertujuan untuk mengetahui efektivitas daya larvasida ekstrak daun dan biji sirsak (Annona muricata L.) terhadap mortalitas larva Aedes aegypti.Metode:Penelitian yang digunakan adalah jenis penelitian trueeksperimental dengan desain penelitian post test control group design. Populasinya adalah  larvaAedes aegypti dengan menggunakan teknik Purposive sampling. Analisa data yang digunakan adalah uji Faktorial dengan nilai p<0,05.Hasil:Berdasarkan data penelitian didapatkan hasil ekstrak daun sirsak (Annona muricata L.) 10% memiliki persentase 50%, ekstrak biji sirsak (Annona muricata L.) 10%memiliki persentase 78,13%, ekstrak kombinasi biji dan sirsak (Annona muricata L.) 10%memiliki persentase 51% terhadap mortalitas larva Aedes aegypti. Simpulan:Didapatkan kesimpulan bahwa ekstrak biji sirsak (Annona muricata L.) 10% paling efektif digunakan sebagai larvasida

scholarly journals Potensi Infusa Daun Sirsak (Annona Muricata) Sebagai Daya Larvasida Larva Aedes aegypti

2017 ◽  
Vol 4 (1) ◽  
pp. 55
Author(s):  
Desto Arisandi ◽  
Maria Theresia Doe Bay ◽  
Nurlaili Farida Muhajir
Keyword(s):  
Aedes Aegypti ◽  
Larvicidal Activity ◽  
Hemorrhagic Fever ◽  
Annona Muricata ◽  
Group Design ◽  
The Third ◽  
Activity Test ◽  
Quasi Experimental ◽  
Global Health Problem ◽  
Active Substances

Background: Dengue hemorrhagic fever (DHF) which is caused by dengue virus remains a global health problem that belongs to Indonesia also. Transmission works through the bite of female Aedes aegypti. An attempt is developing bioinsecticide with no harm which is involved to vector control such as leaves of the soursop (Annona muricata) that contains active substances such as saponins, tannins, and alkaloids that serves as larvicides. Aims: This research attempted to discover soursop leaves infuse with various concentrations served as larvacide to mortality of larva of A. aegypti. Method: This was a quasi-experimental research with posttest group design which conducted in parasitology laboratory of Poltekkes Kemenkes Yogyakarta. The subjects of research that infuse soursop leaves with a concentration of 15% and 20%, while the object of research is the number of deaths the third instar larvae of A. eagypti after addition of various concentrations of soursop leaf infusion. Result: Larvicidal activity test soursop leaf infusion using 15% concentration can kill larvae of A. aegypti by 75%, while 20% concentration can kill larvae of A. aegypti by 85%. Statistical analysis using ANOVA test is known that administration of soursop leaves infuse a significant influence against A. aegypti larvae mortality (p <0.001). Conclusion: Soursop leaves infuse with a concentration of 15% and 20% having a larvicidal activity of A. aegypti.

scholarly journals The Effect Of Bioinsecticide Granule Mixture Of Sugar Apple Seed (Annonae Squamosa) And Betel Leaf (Piper Betle) On Morphology Of Eggs Aedes Aegypti

2021 ◽  
Vol 9 (1) ◽  
pp. 26-30
Author(s):  
Firdha Firdha Novitasari ◽  
Dwi Wahyuni Dwi Wahyuni ◽  
Erma Sulistyaningsih Erma Sulistyaningsih
Keyword(s):  
Aedes Aegypti ◽  
Public Health Problem ◽  
Ordinal Regression ◽  
Seed Extract ◽  
Control Group ◽  
Piper Betle ◽  
Betel Leaf ◽  
Apple Seed ◽  
Regression Test ◽  
Apple Seeds

Introduction: Dengue hemorrhagic fever (DHF) is an important public health problem in Indonesia because its morbidity and mortality are quite high. Prevention by vector control methods with chemical insecticides adversely affects the environment. Research on granular bioinsectide mixture of betel leaf extract and sugar apple seeds against Aedes aegypti is expected to be used to break the chain of Aedes aegypti mosquitoes starting  from the juvenile stage  (eggs) as an effort to control the vector population naturally. Objective: This study aims to analyze the effect of granule bioinsecticide mixture of sugar apple seed extract and betel leaf on changes in the morphology of Aedes aegypti eggs. Methods: This study is a true-experimental study with a Post test Only Control Group Design design. The treatment group will be given a granular bioinsecticide, a mixture of sugar apple seed extract and betel leaf, positive control will be given abate while negative control will be given aquades. Each group uses 25 eggs with 3 repetitions. On day 3, all eggs will be observed using a stereo microscope to determine morphological changes. The data obtained will be analyzed using ordinal regression test. Results: the ordinal regression test got the value of ρ in the Wald test between (0.012 - 0.955)> α = 0.01. Conclusion: there is no effect of mixed granules of betel leaf and sugar apple seeds on the mortality of Aedes aegypti eggs. Further research is needed on new formulations using other natural ingredients to increase the effect of ovicides on Aedes aegypti.

scholarly journals Status Kerentanan Aedes Aegypti Vektor Demam Berdarah Dengue di Kota Padang

2017 ◽  
Vol 6 (2) ◽  
pp. 440
Author(s):  
Kharisma Putra D ◽  
Hasmiwati Hasmiwati ◽  
Arni Amir
Keyword(s):  
Aedes Aegypti ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Post Test ◽  
One Way Anova

Salah satu upaya untuk mengurangi kasus DBD adalah dengan pengendalian vektor DBD dengan larvisida. Temephos merupakan salah satu insektisida yang telah digunakan lebih dari 30 tahun dan berfungsi mengendalikan larva vektor. Penggunaan temephos yang tidak sesuai aturan dapat menyebabkan penurunan kerentanan pada vektor DBD.  Tujuan penelitian ini  adalah untuk  menilai  status  kerentanan larva Aedes aegypti di tiga kecamatan di Kota Padang. Penelitian ini menggunakan post  test  only  with  control  group design. Telur diambil dan dipelihara di laboratorium hingga mencapai larva instar III/IV. Uji kerentanan untuk temephos dilakukan berdasarkan standar WHO. Hasil penelitian menunjukkan pada Kecamatan Kuranji, kematian larva pada konsentrasi 0,005 mg/L sebesar 10%, 0,01 mg/L sebesar 45%, 0,02 mg/L sebesar 86%, dan pada konsentrasi 0,03 mg/L sebesar 100%. Pada Kecamatan Koto Tangah, kematian larva pada konsentrasi 0,005 mg/L sebesar 24%, 0,01 mg/L sebesar 48%, 0,02 mg/L sebesar 99%, dan pada konsentrasi 0,03 mg/L sebesar 100%. Pada Kecamatan Padang Timur pada konsentrasi 0,005 mg/L didapatkan kematian larva sebesar 12%, pada 0,01 mg/L sebesar 43%, pada 0,02 mg/L sebesar 99%, dan pada 0,03 mg/L sebesar 100%. Hasil uji One way-Anova adalah bermakna dengan nilai p<0,05 pada ketiga kecamatan dan LC99 sedikit diatas 0,02 mg/L. Simpulan penelitian ini adalah status kerentanan Aedes aegypti terhadap temephos di tiga kecamatan berkisar antara rentan dan toleran, belum mencapai resisten sehingga temephos masih dapat digunakan dalam pengendalian vektor DBD.

scholarly journals Mosquito Larvacidal Activity of Zingiber montanum Rhizome Extract Against Aedes aegypti larvae

2019 ◽  
pp. 33-40
Author(s):  
Dwi Sulistia Ningrum ◽  
Siwi Pramatama Mars Wijayanti ◽  
Kuswanto Kuswanto
Keyword(s):  
Aedes Aegypti ◽  
Negative Impact ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Larvicidal Effect ◽  
Natural Insecticide ◽  
Post Test ◽  
Quasi Experimental ◽  
Lc50 And Lc90

Aedes aegypti is the main vector which transmits Dengue virus as causing agent of Dengue Haemmorhagic Fever (DHF). Chemical control of Ae. aegypti have an impact on the environment and humans, also burden a high cost. One of the efforts to reduce the negative impact of synthetic insecticide, which is to find out alternative natural insecticide from plant-based insecticides. The purpose of this research is to determine the killing power of the rhizome bangle extract to Ae. aegypti larvae. This research was a quasi-experimental design with post test only control group design. The concentration of extract rhizome bangle used were 0%; 0.125%; 0.25%; 0.5%; 0.75% and 1%. The mosquito sample used in this study were instar III of Ae. aegypti larvae as many as 600 larvae. Data analysis used univariate and bivariate (probit and Kruskal Wallis test). The results showed that there was an effect of the concentration of extract rhizome bangle against the mortality of Ae. aegypti larvae with p=0,002 (p<0,05). Extract rhizome bangle effectively killed Ae. aegypti larvae with LC50 and LC90 were 0.148 % and 0.338 %, with the most effective concentration is 1%. Based on this research, extract rhizome bangle has a larvicidal effect on Ae. aegypti, so it can be used as an alternative method to minimize the usage of chemical larvicides that easily applied by the community.

scholarly journals Efecto tóxico de los extractos de semillas de Annona muricata potenciados con dimetilsulfóxido sobre larvas IV y pupas de Aedes aegypti

2020 ◽  
Vol 27 (2) ◽  
pp. 215-224
Author(s):  
Miguel Constante Bobadilla Alvarez ◽  
Sonia Melinda Reyes Castro
Keyword(s):  
Aedes Aegypti ◽  
Annona Muricata ◽  
Knock Down

El objetivo de este estudio fue demostrar que los principios activos de las semillas de Annona muricata combinados con el extracto etanólico y dimetilsulfóxido (EE-DMSO), incrementan la mortalidad de larvas IV y pupas de Aedes aegypti con relación a extractos acuosos (EA) y extractos etanólicos (EE). Las bioactividades se calcularon por comparación de los porcentajes de mortalidad a las 6, 12, 24 y 48 horas in vitro y campo simulado. Los resultados indicaron mortalidad progresiva dependiente de las concentraciones y tiempos de exposición en larvas y reacción knock-down en pupas. In vitro a 5 mg.L-1, EA y EE ejercieron 100% de mortalidad larvaria en 24 horas de exposición (CL50=46.16 y 19.28 mg.L-1 respectivamente), en contraste con EE-DMSO, que inició sobre 62% con 0.5 mg.L-1 a las 6 horas (CL50=20.33 mg.L-1). La acción pupicida de EA y EE reveló 100% de mortalidad desde 24 horas en todas las concentraciones, a diferencia de EE-DMSO que se alcanzó entre 6 y 12 horas. En campo simulado, EA y EE ejercieron 100% de mortalidad a las 24 horas en larvas (16.91 y 21.21 mg.L-1 ), mientras que en pupas (20.44 y 23.03 mg.L-1) ocurrió a las 12 horas, entretanto, la actividad pupicida de EE-DMSO fue 100% en 6 horas. Los efectos comparativos in vitro y campo simulado denotaron patrones similares de respuestas larvicida y pupicida, pero con mayor sensibilidad en pupas. Los principios activos de las semillas de A. muricata combinados con EE-DMSO potencian la respuesta mortal de larvas y pupas de A. aegypti in vitro y campo simulado.

scholarly journals Effectiveness of Kelor Dung Extract on Aedes aegypti Larvae Mortality

2018 ◽  
pp. 10-15
Author(s):  
Alfina Baharuddin
Keyword(s):  
Aedes Aegypti ◽  
Exposure Time ◽  
Design Method ◽  
Narrative Form ◽  
Control Group ◽  
Control Group Design ◽  
Control Effort ◽  
Average Percentage ◽  
Group Design ◽  
Post Test

The high number of cases of dengue fever in Indonesia, so it needs to be held vector control effort. Moringa is used as a natural anti-mosquito capable of supplying larvae. This is because there are compounds of steroids, triterpenoids, alkaloids, saponins, flavanoids, and tannins in the stem of moringa. The type of research used was experimental with post test only control group design method. The data were collected by counting the number of Aedes aegypti instar III larvae that died in each type of salinity concentration during the exposure time. The experiment was conducted 4 times, Observation, calculation and recording of the number of live and dead larvae every 24, 32, 40 and 48 hours after treatment. The data that has been collected and analyzed is then presented in tabular and narrative form. At the concentration of 3% in the Moringa leather bark estrants at the time of 24 hour measurements in each treatment of 1 (3), 2, 3, 3 (3) treatment, 4 (1) treatment with an average percentage of larva death 27 , 5%. The concentration of 3% extract of Moringa leaf bark effectively kills Aedes aegepty larvae by 45% within 48 hours. Concentration of 3.5% extract of Moringa leaf bark effectively kills Aedes aegepty larva by 45% within 24 hours. The concentration of 4% extract of Moringa leaf bark effectively kills Aedes aegepty larva by 67.5% within 24 hours. The effective exposure time of 24 hours resulted in the death of 67.5% Aedes aegypti.  

scholarly journals Efektivitas Ekstrak Daun Sirih (Piper betle L) dan Ekstrak Bawang Putih (Allium sativum) terhadap Mortalitas Larva Nyamuk Aedes aegypti

bionature ◽  
2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Amirullah Amirullah ◽  
Nurhayu Malik ◽  
Rosmaya Rosmaya
Keyword(s):  
Aedes Aegypti ◽  
Endemic Area ◽  
Leaf Extract ◽  
Allium Sativum ◽  
Larval Mortality ◽  
Hemorrhagic Fever ◽  
Garlic Extract ◽  
Piper Betle ◽  
Mosquito Larvae ◽  
Betel Leaf

Abstract. The study of the efficacy of betel leaf extract (Piper betle L.) and garlic extract (Allium sativum) on the mortality of Aedes aegypti mosquito larvae was carried out at the Zoology Laboratory of the Faculty of Mathematics and Natural Sciences, Halu Oleo Kendari University. Aedes aegypti mosquito larvae were obtained from the rearing of F2 from Aedes aegypti eggs that were taken by ovitrap from in Kambu Village, Kendari City, which is an endemic area of Dengue Hemorrhagic Fever (DHF). Extrac of betel leaf (Piper betle L.) and garlic extract (Allium sativum) at concentrations of 1.0%, 2.0% and 3.0% respectively and their combinations were given to Aedes aegypti larvae (L4). The results showed that the highest larval mortality (88%) occurred after 24 hours of exposure to betel leaf extract (Piper betle L.) at a concentration of 3%, whereas with garlic extract (Allium sativum) the highest larval mortality (98%) occurred at concentration 3 %, and for the highest larval mortality (99%) found in a combination of 3% betel leaf extract (Piper betle L.) and 3% garlic extract (Allium sativum). Based on WHO (2009), the most effective treatment for killing Aedes aegyti larvae is 3% garlic (Allium sativum) extract and a combination of 3% betel leaf extract (Piper betle L.) and 3% garlic extract (Allium sativum).Keywords:  Biopesticide, Mortality, Aedes aegypti, betle leaf (Piper betle L.), garlic (Allium sativum).

scholarly journals Hemostatic Effect of Ethanol Extract of Piper betle, Linn Leaves to Male Mice

Molekul ◽  
2017 ◽  
Vol 12 (1) ◽  
pp. 23
Author(s):  
Sadakata Sinulingga ◽  
Subandrate Subandrate ◽  
Bebbi Arisya Kesumaputri ◽  
Galuh Anggraini
Keyword(s):  
Bleeding Time ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Piper Betle ◽  
Control Group Design ◽  
Minimum Concentration ◽  
Group Design ◽  
Hemostatic Effect

Hemorrhage occurs in most of the dental care. Untreated hemorrhage could cause excessive blood loss, hypotension, and cyanosis. A Natural resource that reported has an hemostatic effect is ethanol extract of betel leaves (Piper betel, Linn).The aim of this study is to find the minimum concentration of ethanol extract of betel leaves which capable of shortening the bleeding time in mice. The experimental study used pretest-posttest with control group design was conducted on 35 mice that divided into 7 group which are negative control, positive control (feracrylum 1%), the ethanol extract of betel leaves 1%, 5%, 10%, 15%, and 20%. All mice were injected heparin intravenously. Mice’s tail was cut at diameter 3 mm and pretest bleeding time was counted. Mice’s tail was recut at diameter 4 mm, given treatment for 5 seconds and posttest bleeding time was counted. Results of paired t-test showed that reduction of bleeding time between pretest and posttest was significant (p<0,050). The enhancement of ethanol extract of betel leaves concentration leads to better hemostatic effect. Results of ANOVA test showed that comparison of posttest bleeding time among groups was significant (p<0,050). The minimum concentration of ethanol extract of betel leaves which capable of shortening the bleeding time in mice is 5%.

scholarly journals STATUS RESISTENSI LARVA AEDES SP TERHADAP LARVASIDA SEBAGAI FAKTOR RISIKO KEJADIAN DEMAM BERDARAH DENGUE DI WILAYAH BUFFER PELABUHAN LAUT TANJUNG PERAK SURABAYA

2020 ◽  
Vol 4 (1) ◽  
pp. 46-54
Author(s):  
Fawandi Eta Rachmawati
Keyword(s):  
Aedes Aegypti ◽  
Resistance Ratio ◽  
Susceptibility Test ◽  
Control Group ◽  
Control Group Design ◽  
Purposive Sampling ◽  
Group Design

Pelabuhan baik darat maupun udara selain sebagai pintu masuk orang, alat dan barang juga sebagai media transportasi vektor penyebab penyakit dalam siklus penularan penyakit pada manusia khususnya penyakit demam berdarah. Vektor penyakit (Aedes aegypti) dapat berpindah dari daerah endemis ke daerah lain akibat terbawa oleh barang atau alat angkut dari adanya aktifitas di pelabuhan. IHR 2005 menyatakan wilayah perimeter pelabuhan harus terbebas dari jentik dengan House Index (HI)=0. Pada wilayah buffer nilai House Index (HI) >1, jika melebihi dari angka tersebut maka diharuskan melakukan upaya pengendalian vektor. Dari fenomena tersebut peneliti ingin mengetahui status resistensi dari larva Aedes Sp di wilayah buffer pelabuhan Tanjung Perak Surabaya. Penelitian bersifat eksperimen murni dengan rancangan Postest Only with Control Group Design menggunakan metode susceptibility test WHO. Pengambilan data menggunakan metode purposive sampling di wilayah buffer pelabuhan Tanjung Perak. Jumlah larva yang mati saat percobaan dilakukan perhitungan nilai LC 50, LC 90 dan LC 99 menggunakan regresi logistik Probit test, sehingga dapat diketahui nilai Resistance Ratio (RR) Nilai RR larva Aedes Sp di wilayah buffer termasuk dalam kategori rentan hingga resisten sedang. Penggunaan larvasida temephos masih bisa dilakukan di wilayah buffer, namun perlu upaya edukasi kepada masyarakat di wilayah pelabuhan agar dapat menggunakan larvasida dengan aman dan tepat guna.

Sign in / Sign up

Export Citation Format

Share Document