The Effect Of Bioinsecticide Granule Mixture Of Sugar Apple Seed (Annonae Squamosa) And Betel Leaf (Piper Betle)  On Morphology Of Eggs Aedes Aegypti

Introduction: Dengue hemorrhagic fever (DHF) is an important public health problem in Indonesia because its morbidity and mortality are quite high. Prevention by vector control methods with chemical insecticides adversely affects the environment. Research on granular bioinsectide mixture of betel leaf extract and sugar apple seeds against Aedes aegypti is expected to be used to break the chain of Aedes aegypti mosquitoes starting from the juvenile stage (eggs) as an effort to control the vector population naturally. Objective: This study aims to analyze the effect of granule bioinsecticide mixture of sugar apple seed extract and betel leaf on changes in the morphology of Aedes aegypti eggs. Methods: This study is a true-experimental study with a Post test Only Control Group Design design. The treatment group will be given a granular bioinsecticide, a mixture of sugar apple seed extract and betel leaf, positive control will be given abate while negative control will be given aquades. Each group uses 25 eggs with 3 repetitions. On day 3, all eggs will be observed using a stereo microscope to determine morphological changes. The data obtained will be analyzed using ordinal regression test. Results: the ordinal regression test got the value of ρ in the Wald test between (0.012 - 0.955)> α = 0.01. Conclusion: there is no effect of mixed granules of betel leaf and sugar apple seeds on the mortality of Aedes aegypti eggs. Further research is needed on new formulations using other natural ingredients to increase the effect of ovicides on Aedes aegypti.

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The Effect of Sugar Apple (Annona squamosa L.) Seeds Solution as an Insecticide Against Mortality of Aedes aegypti Mosquito with Liquid Electric Method

JURNAL KESEHATAN LINGKUNGAN ◽

10.20473/jkl.v9i2.2017.200-208 ◽

2018 ◽

Vol 9 (2) ◽

pp. 200

Author(s):

Amalinda Mega Novasari ◽

Retno Sasongkowati

Keyword(s):

Aedes Aegypti ◽

Public Health Problem ◽

Hemorrhagic Fever ◽

Cross Sectional Study ◽

Primary Data ◽

Control Group ◽

Annona Squamosa ◽

Control Group Design ◽

Cross Sectional ◽

Apple Seeds

Dengue hemorrhagic fever is a public health problem in Indonesia that the number of DHF cases has been increased. Morbidity number of DHF per 100,000 population at 2011 was 26.67 became 34.3 in 2012. The control of DHF has been attempted, but a lot of synthetic insecticide used has been negatively affected to environment. So, it need the insecticide alternative. The aim of study was to identify the effect of solvent sugar apple (Annona squamosa L.) seeds to the death of Aedes aegypti mosquitoes by electric liquid method. Type of this research was experimental with cross sectional study design as well as the post test only control group design. The study was conducted from February to July 2013 in the Laboratory of Entomology of the Provincial Health Office of East Java. The population of this research was the Aedes aegypti with 560 sample of mosquitoes were divided into five treatments and four repetitions for each treatment. The collection of quantitative data drawn from primary data through observation by calculating the death of Aedes aegypti of each the sugar apple seeds concentration of 10%, 20%, 30%, 40% and 50% for 24 hours. The results showed that the concentration of 50% solvent of sugar apple seeds provide the greatest effect in causing the death of Aedes aegypti mosquitoes as many as 36 died after exposure. It can be concluded that there are effected by giving solvent of sugar apple (Annona squamosa L.) seeds to the death of Aedes aegypti mosquitoes by electric liquid method.

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Efektivitas Ekstrak Biji Bintaro (Cerbera manghas) sebagai Larvasida Hayati pada Larva Aedes aegypti Instar III

Jurnal Kesehatan ◽

10.26630/jk.v9i2.889 ◽

2018 ◽

Vol 9 (2) ◽

pp. 218

Author(s):

Khoiriyanti Wulandari ◽

Mei Ahyanti

Keyword(s):

Aedes Aegypti ◽

Dengue Hemorrhagic Fever ◽

Contact Time ◽

Statistical Tests ◽

Hemorrhagic Fever ◽

Seed Extract ◽

Control Group ◽

Control Group Design ◽

Post Test ◽

Cerbera Manghas

<p>Dengue Hemorrhagic Fever (DHF) or Dengue Hemorrhagic Fever (DHF) is a disease caused by a virus which is very dangerous because it can cause the patient to die within a few days. Bandar Lampung city itself is a dengue endemic area. According to data from the Lampung Provincial Health Office, in 2015, from 15 there were 2,996 deaths in 31 people. The study aimed to knowing the effectiveness of bintaro seed extract (Cerbera manghas) as larvacide in III instars Aedes aegypti larvacides experimental research with post test only control group design. The sampling method was purposive sampling. Independent variables were bintaro seed extract (dose and time), the dependent variable was Aedes aegypti larvAedes The results showed that from 5 types of doses with 5 repetitions, the death result of Aedes aegypti larvae with a dose of 0% had no mortality, a dose of 0.1% with an average of 12.55, a dose of 0.5% with an average of 15.15 , 1% dose with an average of 18.20, a dose of 1.5% with an average of 22.15, and a dose of 2% with an average of 25. At the time of contact, the death result of the larvae was found with an average 6-hour contact time. an average of 8.70, 12 hours with an average of 14.13, 24 hours with an average of 18.37 and 48 hours with an average of 20.83. According to the results of statistical tests, the most effective effect of bintaro seed extract dosage is at a dose of 1.5% and contact time of 6 hours.<strong></strong></p>

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Efektivitas Ekstrak Daun Sirih (Piper betle L) dan Ekstrak Bawang Putih (Allium sativum) terhadap Mortalitas Larva Nyamuk Aedes aegypti

bionature ◽

10.35580/bionature.v20i1.9760 ◽

2019 ◽

Vol 20 (1) ◽

Author(s):

Amirullah Amirullah ◽

Nurhayu Malik ◽

Rosmaya Rosmaya

Keyword(s):

Aedes Aegypti ◽

Endemic Area ◽

Leaf Extract ◽

Allium Sativum ◽

Larval Mortality ◽

Hemorrhagic Fever ◽

Garlic Extract ◽

Piper Betle ◽

Mosquito Larvae ◽

Betel Leaf

Abstract. The study of the efficacy of betel leaf extract (Piper betle L.) and garlic extract (Allium sativum) on the mortality of Aedes aegypti mosquito larvae was carried out at the Zoology Laboratory of the Faculty of Mathematics and Natural Sciences, Halu Oleo Kendari University. Aedes aegypti mosquito larvae were obtained from the rearing of F2 from Aedes aegypti eggs that were taken by ovitrap from in Kambu Village, Kendari City, which is an endemic area of Dengue Hemorrhagic Fever (DHF). Extrac of betel leaf (Piper betle L.) and garlic extract (Allium sativum) at concentrations of 1.0%, 2.0% and 3.0% respectively and their combinations were given to Aedes aegypti larvae (L4). The results showed that the highest larval mortality (88%) occurred after 24 hours of exposure to betel leaf extract (Piper betle L.) at a concentration of 3%, whereas with garlic extract (Allium sativum) the highest larval mortality (98%) occurred at concentration 3 %, and for the highest larval mortality (99%) found in a combination of 3% betel leaf extract (Piper betle L.) and 3% garlic extract (Allium sativum). Based on WHO (2009), the most effective treatment for killing Aedes aegyti larvae is 3% garlic (Allium sativum) extract and a combination of 3% betel leaf extract (Piper betle L.) and 3% garlic extract (Allium sativum).Keywords: Biopesticide, Mortality, Aedes aegypti, betle leaf (Piper betle L.), garlic (Allium sativum).

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EFEKTIVITAS ABATE DAN EKSTRAK DAUN SIRIH (Piper betle) DALAM MEMATIKAN LARVA Aedes Aegypti L INSTAR III

Masker Medika ◽

10.52523/maskermedika.v8i1.398 ◽

2020 ◽

Vol 8 (1) ◽

pp. 226-232

Author(s):

Triana Oktaviani ◽

Zairinayati Zairinayati

Keyword(s):

Aedes Aegypti ◽

Leaf Extract ◽

Hemorrhagic Fever ◽

Piper Betle ◽

Mosquito Larvae ◽

Betel Leaf ◽

Preventive Effort ◽

Randomized Design ◽

Natural Insecticides ◽

Completely Randomized Design

Latar Belakang: Aedes aegypti merupakan nyamuk yang dapat berperan sebagai vektor Demam Berdarah Dengue (DBD). Upaya promotif dan preventif menjadi mutlak dilakukan untuk memutus mata rantai penularan dengan membunuh larva nyamuk dengan larvasida alami menggunakan ekstrak daun sirih (Piper betle) sebagai insektisida alami. Tujuan dari penelitian: ini adalah untuk mengetahui perbedaan abate dan ekstrak daun sirih (Piper betle) dalam mematikan larva Aedes aegypti L instar III. Desain penelitian: ini bersifat eksperimen dengan menggunakan rancangan acak lengkap (RAL) yang terdiri dari 3 perlakuan 1 kontrol dan 8 kali pengulangan percobaan. Hasil Penelitian: dapat diketahui bahwa untuk penggunaan ekstrak daun sirih (Piper betle) dengan konsentrasi 5% dapat mematikan larva sebanyak 8 ekor, sedangkan konsentrasi 10% tidak dapat mematikan larva (0 larva) dan konsentrasi 15% dapat mematikan larva sebanyak 17 (85%) sedangkan untuk penggunaan abate dengan konsentrasi 5%, 10% dan 15% dapat mematikan larva sebanyak 160 larva (100%). Kesimpulan: bahwa abate mempunyai efektifitas lebih tinggi di banding dengan ekstrak daun sirh (Piper betle). Background: Aedes aegypti is a mosquito that can act as a vector of dengue Hemorrhagic Fever (DHF). Promotove and preventive effort are absolutely necessary to break the chain to transmission by killing mosquito larvae with natural larvacides using betel leaf extract (Piper betle) as a natural insecticides. The purposes of this study: this was to determine the differences in abate and betel leaf extract (Piper batle) in kliing Aedes aegypti instar III larvae. Research methods this is an experimental study using a completely randomized design (CRD) consisting of 3 treatments 1 control and 8 repetitions of the experiment. The results: it can be seen that for the use of betel leaf extract(Piper betle) with a concentration cannot kill larvae in 17 (85%) whilke for the use of abate with a concentration of 5%, 10% and 15% can kill larvae of 160 (100%). Conclusion: that the abate has a higher effectiveness compared to sirih leaf extract.

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Biolarvasides of Cananga odorata Flower and Zyzygium polyanthum Leaves Ethanol Extract on Aedes aegypti Larva

Medical Laboratory Technology Journal ◽

10.31964/mltj.v1i1.304 ◽

2020 ◽

Vol 1 (1) ◽

Author(s):

Nurlailah Nurlailah ◽

Anny Thuraidah

Keyword(s):

Aedes Aegypti ◽

Leaf Extract ◽

Public Health Problem ◽

Ethanol Extract ◽

Hemorrhagic Fever ◽

Wilcoxon Test ◽

Control Group ◽

Control Group Design ◽

Flower Extract ◽

Negative Impacts

Dengue hemorrhagic fever (DHF) is one of the diseases caused by Aedes Aegypti mosquitoes. DHF is still a public health problem, including in Indonesia, due to the high mortality rate caused by this mosquito. The risk caused by the bite of the Aedes sp mosquito is quite dangerous, including acute fever, bleeding, shock, and even death, so that efforts are needed to control it. One of the natural ingredients that can use to eradicate Aedes Aegypti mosquitoes without causing negative impacts on the environment is the Cananga flower and Zyzigium Leaves. This study purposes of determining the optimum concentration of Cananga Flowers and Zyzigium Leaves Ethanol extract of which was having biolarvaside effect to Aedes Aegypti larvae. The method used a simple experiment with Posttest Only With Control Group Design. The data result of the research analyzed with Logit and Wilcoxon test. Achievement of those tests was LC50 Zyzigium leaf extract on contact for 24 hours was 0.609-0.935, and LC99 was between 1.064-1.973 while Cananga flower extract obtained LC50 of 0.228-0.261 and the LC99 was 0.446 to 0.558. Wilcoxon test for compared bioalarvaside effect results between Cananga Flower and Zyzigium Leaf received a significance value less than α (0.001<0.05), so it can ascertain that the Cananga Flower extract was stronger biolarvaside than Zyzigium Leaf extract.

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EFEKTIVITAS KOMBINASI EKSTRAK DAUN SIRIH MERAH (Piper Crocatum) DAN EKSTRAK BIJI ALPUKAT (Persea americana) DALAM MENGHAMBAT PERTUMBUHAN Candida albicans

Jurnal Kimia Riset ◽

10.20473/jkr.v2i2.6196 ◽

2017 ◽

Vol 2 (2) ◽

pp. 86 ◽

Cited By ~ 1

Author(s):

Vini Anggraini ◽

Masfufatun Masfufatun

Keyword(s):

Candida Albicans ◽

Leaf Extract ◽

Seed Extract ◽

Persea Americana ◽

Control Group ◽

Control Group Design ◽

Group Design ◽

Betel Leaf ◽

Post Test ◽

Avocado Seed

Abstrak Candida albicans merupakan salah satu mikrooganisme penyebab infeksi kandidiasis. Daun sirih merah (Piper crocatum) dan biji alpukat (Persea americana) telah diteliti dapat menghambat pertumbuhan Candida albicans. Tujuan penelitian ini adalah untuk mengetahui efektivitas kombinasi daun sirih merah (Piper crocatum) dan ekstrak biji alpukat (Persea americana) dalam menghambat pertumbuhan Candida albicans. Penelitian bersifat eksperimental laboratorium (true experiment) dengan pendekatan post test control group design only. Daun sirih merah dan biji alpukat diekstraksi secara maserasi menggunakan pelarut etanol. Pada masing-masing ekstrak etanol dilakukan uji pendahuluan untuk mengetahui konsentrasi optimum ektrak dalam memnghambat pertumbuhan C. albicans. Daya Hambat pertumbuhan C. albicans diuji menggunakan metode difusi. Hasil penelitian menunjukkan bahwa ekstraks daun sirih merah dan biji alpukat masing-masing memiliki daya hambat terbesar pada konsentrasi 10%. Kombinasi ekstrak daun sirih merah dan ekstrak biji alpukat memiliki daya hambat yang signifikan terhadap pertumbuhan Candida albicans dibandingakan kontrol negatif, kontrol positif dan ekstrak daun sirih merah. Dengan demikian kombinasi ekstrak daun sirih dan biji aplukat etanol berpotensi sebagai antifungi dalam menghambat pertumbuhan C. albicans sehingga diharapkan bisa mengurangi prevalensi kandidiasis. Kata Kunci : daun sirih merah, biji alpukat, Candida albicans, Abstract Candida albicans is one of the microbialism that causes candidiasis infection. Red betel leaf (Piper crocatum) and avocado seed (Persea americana) have been studied to inhibit the growth of C. albicans. The purpose of this study was to determine the effectiveness of red betel leaf (Piper crocatum) and avocado seed extract (Persea americana) combination in inhibiting the growth of C. albicans. Research is experimental laboratory (true experiment) with post test approach control group design only. Red betel leaf and avocado seeds are extracted by maceration using ethanol solvent. In each ethanol extract, a preliminary test was performed to find out the optimum concentration of extract in inhibiting the growth of C. albicans. C. albicans growth retardant was tested using diffusion method. The results showed that the extract of red betel leaf and avocado seeds each had the greatest inhibitory concentration at 10%. The combination of red betel leaf extract and avocado seed extract have significant inhibitory effect on C. albicans growth compared to negative control, positive control and red betel leaf extract. Thus, the combination of betel leaf extract and ethanol seed has the potential as an antifungal in inhibiting the growth of C. albicans so it is expected to reduce the prevalence of candidiasis. Keywords: red betel leaf, avocado seed, Candida albicans,

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Perbandingan Efektivitas Pencampuran Ekstrak Daun Sirih (Piper Betle) dan Daun Bengkuang (Pachyrhizus Erosus) dengan Abate Terhadap Mortalitas Larva Aedes Aegypti

Jurnal Ilmu Kesehatan ◽

10.30650/jik.v5i2.60 ◽

2017 ◽

Vol 5 (2) ◽

pp. 113-120

Author(s):

Marjan Wahyuni ◽

Rusdi Rusdi

Keyword(s):

Aedes Aegypti ◽

Leaf Extract ◽

Disease Vectors ◽

Piper Betle ◽

Air Humidity ◽

The Public ◽

Betel Leaf ◽

Pachyrhizus Erosus ◽

Number Of Treatment ◽

Different Doses

Dengue Haemorrhagic Fever (DHF) is a disease that is still a major problem in Indonesia. Until now there is no cure or vaccine to combat dengue, so the only way is to break the chain of transmission of mosquito Aedes aegypti is the prevention or control of disease vectors. This study aims to determine the Efficiency of Mixed Leaf Extract (Pipper Betle) and Leaf Yam Extract with Temephos On Mortality of Aedes aegypti. This research is experimental and research will be conducted in the laboratory of environmental health. The number of treatment in this study was 3 treatment with different doses of 0.5 mg / l ; 1.0 mg / l ; 1.5 g / l ; 2m g / l and 1 control (0 mg / liter) were treated in the larvae mosquitoes. The results showed that the air temperature at the study site averaged 27oC, the air humidity between 78-79%, the water temperature that became the Aedes aegypti breeding 26-27oC, the average pH 6.8-7. The results showed that the leaf extract beta and bengkuang leaves at a dose of 2.0 mg / l for 24 hours were able to kill Aedes aegypti larvae of 96.6%, while abates with a dose of 2.0 mg / l for 24 hours were able to kill Aedes aegypti larva by 70%. After statistical analysis with Independent t-test showed that betel leaf extract and bengkuang leaf gave a significant effect on the mortality of Aedes aegypti larvae. So it can be concluded that there is a significant effectiveness difference between bengkuang leaf extract (Pachyrrhyzus erosus) and betel leaf (Piper betle) with abate to mortality of Aedes aegypti larvae. for the mortality of Aedes aegypti larvae both in terms of average mortality (p = 0.003) and in terms of mean duration (p = 0,000) and there was a significant relationship (p = 0,000) between giving both larvacides to Aedes aegypti larvae mortality. This research suggests to the public to use organic larvacides and to reduce the use of abate.

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Uji Toksisitas Ekstrak Biji Kelor (Moringa Oleifera) Terhadap Larva Nyamuk Aedes Aegypti

Jurnal Farmasi Tinctura ◽

10.35316/tinctura.v1i2.987 ◽

2020 ◽

Vol 1 (2) ◽

pp. 34-44

Author(s):

Neneng Bisyaroh

Keyword(s):

Aedes Aegypti ◽

Moringa Oleifera ◽

Negative Control Group ◽

Negative Control ◽

Seed Extract ◽

Control Group ◽

Group Approach ◽

Larvicidal Effect ◽

Post Test ◽

Moringa Oleifera Seed

In Indonesia, there are many plants that contain secondary metabolite compounds which can be a source of vegetable insecticides which can be used for vector control. Plants that can be used as vegetable larvicides include moringa seed extract containing secondary metabolites in the form of alkaloids and phenolic compounds, namely saponins and tannins. These compounds are larvicidal against Aedes aegypti larvae. The purpose of this study was to determine the level of toxicity of Moringa oleifera seed extract to mortality of Aedes aegypti mosquito larvae. This type of research is a laboratory experimental study with a post test only control group approach, using 6 groups (negative control group, 500 ppm moringa seed extract, 750 ppm moringa seed extract, 1000 ppm moringa seed extract, 1250 ppm moringa seed extract, and 1250 ppm seed extract. moringa 1500 ppm). The results showed that the extract of Moringa oleifera seeds had a larvicidal effect on 3rd / IV instar Aedes aegypti larvae. 62.64%) while the lowest value was at a concentration of 500 ppm with 3.33 dead larvae (13.32%). Keywords: Moringa seeds, toxicity, Aedes aegypti larvae ABSTRAK Di Indonesia banyak memiliki tanaman yang memiliki kandungan senyawa metabolit sekunder yang dapat menjadi sumber bahan insektisida nabati yang dapat dimanfaatkan untuk pengendalian vektor. Tanaman yang dapat digunakan sebagai larvasida nabati antara lain yaitu ekstrak biji kelor mengandung senyawa metabolit sekunder berupa senyawa alkaloid dan senyawa fenolik yaitu saponin dan tannin. Senyawa-senyawa tersebut bersifat larvasida terhadap larva Aedes aegypti. Tujuan penelitian ini untuk Tujuan dari penelitian ini yaitu untuk mengetahui tingkat toksisitas dari ekstrak biji kelor (Moringa oleifera) terhadap mortalitas larva nyamuk Aedes aegypti. Jenis penelitian ini adalah penelitian eksperimental laboratoris dengan pendekatan Post test only control group, menggunakan 6 kelompok (kelompok kontrol negatif, ekstrak biji kelor 500 ppm, ekstrak biji kelor 750 ppm, ekstrak biji kelor 1000 ppm, ekstrak biji kelor 1250 ppm, dan ekstrak biji kelor 1500 ppm). Penelitian ini diperoleh hasil yaitu ekstrak biji kelor (Moringa oleifera) memiliki efek larvasida terhadap larva Aedes aegypti instar III/IV jumlah kematian larva Aedes aegypti instar III/ IV memperlihatkan bahwa jumlah kematian larva tertinggi terdapat pada konsentrasi 1500 ppm yaitu sebanyak 15,66 ekor (62,64%) sedangkan nilai terendah terdapat pada konsentrasi 500 ppm dengan larva yang mati sebanyak 3,33 ekor (13,32%). Kata Kunci: Biji Kelor, Toksisitas, Larva Aedes aegypti

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The Effect of Green Betel Leaves (Piper betle L.) Extract on Wounding Healing in Mice (Mus musculus L.)

JURNAL BIOLOGI TROPIS ◽

10.29303/jbt.v21i1.2282 ◽

2021 ◽

Vol 21 (1) ◽

pp. 103

Author(s):

Nurul Atika Zar’ah ◽

Syachruddin Syachruddin ◽

Kusmiyati Kusmiyati

Keyword(s):

Wound Healing ◽

Mus Musculus ◽

Leaf Extract ◽

Block Design ◽

Average Diameter ◽

Control Group ◽

P Value ◽

Piper Betle ◽

Betel Leaf ◽

The One

Green betel leaf (Piper betle L.) contains secondary metabolites, namely alkaloids, flavonoids, steroids, terpenoids, saponins, tannins. Green betel leaf (Piper betle L.) is widely used for wound healing, especially in rural Indonesians. This study aims to determine the effect of green betel leaves extract (Piper betle L.) on wound healing in mice (Mus musculus L.). The samples were determined using a randomized block design (RBD) using mice (Mus musculus L.) which consisted of three treatment groups using a concentration of 20%, 30%, 40% and one control group. The Measure of the average diameter of the wound was using a ruler on each side of the diameter. The data measurement for the average diameter of wound restriction was analyzed by using the one way. The one way Anova test showed that the sig or p-value is 0.000 <0.05 (at the alpha level of 5%) so that Ho is rejected which indicates that there is an effect of giving green betel leaf extract (Piper betel L.) on wound healing in mice (Mus musculus L.). Based on the results of the LSD or LSD test, it shows that it is not significant / the same because the sig value is 0.074> 0.05, which means that no change occurs in mice when given 30% or 40% extract. Green betel leaf extract (Piper betle L.) has an effect on wound healing in mice (Mus musculus L.).

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PENGARUH LARUTAN EKSTRAK DAUN SIRIH (Piper betle L.) 50% TERHADAP PELEPASAN ION METAL (Ni, Cr dan Fe) PADA BREKET ORTODONTIK

ODONTO Dental Journal ◽

10.30659/odj.4.1.32-37 ◽

2017 ◽

Vol 4 (1) ◽

pp. 32

Author(s):

Marzuki Akbar J. Dundu ◽

Grahita Aditya ◽

Eko Hadianto

Keyword(s):

Inductively Coupled Plasma ◽

Metal Ion ◽

Leaf Extract ◽

Artificial Saliva ◽

Negative Control ◽

Control Group ◽

Piper Betle ◽

Orthodontic Bracket ◽

Betel Leaf ◽

Significant Difference

Background: Orthodontic bracket is one of the components in a fixed orthodontic treatment. The influence of the oral cavity of an orthodontic bracket is a type IV hypersensitivity reaction due to corrosion on the bracket. The solution of extracts of betel leaf (Piper betle L.) of 50% which could affect corrosion. This study aimed to know the effect of betel leaf extract solution (piper betle L.) 50% against metal ion release.Method: The research method was “The Post Test Control Group Desain”, consists of four large groups respectively soaked with 6 units of brackets, consisting of a solution of betel leaf extract (piper betle L.) 50% (treatment), aquabidest (negative control), artificial saliva (negative control) and Naf (positive control) incubated for 7 days with a temperature of 370 C. Then ion measurement using the tool inductively coupled plasma – optical emission spectrophotometry (ICP-OES).Results: this study showed that the average release of ion the largest was on the solution of betel leaf extract (0.157167) then aquabides (0.086967), artificial saliva (0,08833) and sodium flouride (0.005533). Test results of Mann-Withney showed the presence of significant difference (p<0.05) release of ion Ni, Cr and Fe on four groups except among artificial saliva groups and sodium fluoride but the group that was soaked in a solution of betel leaf extract (piper betle L.) 50% occured a release of ion Ni, Cr and Fe higher. This is because the samples werestored in solution of betel leaf extract (Piper betle L.) of 50% which is a liquid electrolyte that can cause ion release.Conclusion: Betel leaf extract (piper betle L.) 50% is not effective to be used as an alternative to slow corrosive to metal ion (Ni, Cr and Fe) on a metal bracket.

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