scholarly journals CHARACTERISTICS OF miRNA INTERACTION WITH mRNA OF ISCHEMIC HEART DISEASE CANDIDATE GENES

REPORTS ◽  
2021 ◽  
Vol 335 (1) ◽  
pp. 74-82
Author(s):  
D.D. Mukushkina ◽  
S. Labeit ◽  
A.T. Ivashchenko
Keyword(s):  
Free Energy ◽  
Heart Disease ◽  
Ischemic Heart Disease ◽  
Candidate Genes ◽  
Target Genes ◽  
Molecular Genetic ◽  
Ischemic Heart ◽  
Development Processes ◽  
Energy Of Interaction ◽  
And Oxidative Stress

Ischemic heart disease (IHD) is the most serious cardiovascular disease and one of the leading causes of death worldwide. An important role in the pathophysiology of IHD play such processes as the processes of inflammation and immune response, metabolism of homocysteine and folate, development processes of endothelial dysfunction and oxidative stress and homeostasis system. Accordingly, the identified genes that are directly involved in these processes. In addition, miRNA (mRNA-inhibiting RNA) may affect the expression of these candidate genes. Using bioinformatics methods, the most efficient associations of miRNA and target genes were established. This research presents the characteristics of miRNA interactions with mRNA of candidate IHD genes. Candidate genes were identified that had a free energy of interaction with miRNA equal to -120 kJ / mole and higher in the following interactions: in 5’UTR - ALDH2 and ID02142.3p-miR; CELSR2 and ID00457.3p-miR; DDAH2 and ID01272.3p-miR; DNMT1 and ID02052.5p-miR; DOCK7 and ID00061.3p-miR; EGFR and ID02457.3p-miR; FOLH1 and ID01428.3p-miR; IL6R and miR-6089; NOS3 and ID02363.5p-miR; NPC1 and ID00551.3p-miR; PPP1R17 and ID01693.5p-miR; PRKCH and ID00520.5p-miR; SERPINE1 and ID01098.3p-miR; in CDS - ABCG8 and ID03064.3p-miR; ADORA2A and ID02697.3p-miR; APOA1 and ID00457.3p-miR; CDKN2B and ID02899.3p-miR; IL6R and ID01806.3p-miR; TIMP2 and ID00098.5p-miR; TNF and ID02050.3p-miR; TRIB1 and ID03208.5p-miR; VWF and ID01238.5p-miR. Associations were also revealed in the 3'UTR region with an interaction free energy of -115 kJ/mole and higher: AGTR2 and ID01213.5p-miR; APLNR and ID00616.5p-miR; CXCL12 and ID00483.3p-miR; FADS2 and miR-1224-3p; FCGR2A and miR-1273g-3p; GCKR and ID02928.3p-miR; IL6R and ID00913.5p-miR; KCNJ11 and ID03288.5p-miR; PPP1R3B and ID00913.5p-miR; TFPI and miR-1273g-3p; TIMP2 and ID01941.5p-miR. The results obtained could be used as molecular genetic markers of IHD for the diagnosis of this disease.

scholarly journals Association of metabolic syndrome and oxidative stress with ischemic heart disease in middle-aged persons

Medicina ◽  
2008 ◽  
Vol 44 (5) ◽  
pp. 392 ◽  
Author(s):  
Liucija Černiauskienė ◽  
Dalia Lukšienė ◽  
Abdonas Tamošiūnas ◽  
Regina Rėklaitienė ◽  
Lilija Margevičienė
Keyword(s):  
Oxidative Stress ◽  
Metabolic Syndrome ◽  
Heart Disease ◽  
Vitamin E ◽  
Ischemic Heart Disease ◽  
Odds Ratio ◽  
Ischemic Heart ◽  
Middle Aged ◽  
Men And Women ◽  
And Oxidative Stress

The aim of this study was to evaluate the association of metabolic syndrome and oxidative stress with ischemic heart disease in middle-aged persons (men and women aged 45–64 years). Material and methods. In this study, we have used data of 533 persons (247 men and 286 women) aged 45– 64 years from Kaunas population cohort investigated according to WHO MONICA study protocol during 2001–2002 in whom concentrations of malondialdehyde, a marker of lipid peroxidation, and antioxidant vitamin E in blood serum were determined. Metabolic syndrome was defined by the criteria of Adult Treatment Panel III. Oxidative stress was determined in the presence of increased level of malondialdehyde (≥5 mmol/L) and decreased level of lipid-standardized vitamin E (<20 mmol/L). The levels of malondialdehyde and vitamin E were measured by fluorimetric methods. Results. Ischemic heart disease was diagnosed in 19.8% of men and 21.3% of women. The frequency of ischemic heart disease among persons (men and women) with metabolic syndrome was significantly higher than among persons without metabolic syndrome (27.4% vs. 17.8%, respectively; odds ratio, 1.63; P=0.032). The frequency of ischemic heart disease among persons with oxidative stress was significantly higher than among persons without oxidative stress (29.1% vs. 17.6%, respectively; odds ratio, 1.88; P=0.029). The highest prevalence of ischemic heart disease (30.0%) was among persons with metabolic syndrome and oxidative stress, and the lowest prevalence of this disease (13.8%) was among persons without metabolic syndrome and without oxidative stress (odds ratio, 2.54; P=0.017). These data were adjusted by sex and age. Conclusion. Metabolic syndrome and oxidative stress are significantly associated with ischemic heart disease among 45–64-year-old persons.

Ischemic Heart Disease in Subjects of Young Age: Current State of the Problem. Features of Etiology, Clinical Manifestation and Prognosis

Kardiologiia ◽  
2018 ◽  
Vol 58 (11) ◽  
pp. 24-34 ◽  
Author(s):  
E. Yu. Andreenko ◽  
I. S. Yavelov ◽  
М. М. Loukianov ◽  
A. N. Vernohaeva ◽  
O. M. Drapkina ◽  
...  
Keyword(s):  
Risk Factors ◽  
Heart Disease ◽  
Ischemic Heart Disease ◽  
Molecular Genetic ◽  
Young Patients ◽  
Ischemic Heart ◽  
Coronary Syndrome ◽  
Current State ◽  
Monogenic Diseases ◽  
Conventional Risk Factors

In addition to conventional risk factors in young patients with ischemic heart disease (IHD) numerous other risk factors including genetics play an important role in its causation. Molecular genetic testing is recommended for the detection of monogenic diseases with a high risk of developing IHD, such as familial hypercholesterolemia. In majority ofyoung patients, the first manifestation of IHD is an acute coronary syndrome. Young patients with IHD more often have normal coronary arteries or single-vessel coronary disease, and in up to 20% of them cause of myocardial ischemia is not related to atherosclerosis. In general, young patients with IHD have better prognosis. However, there are sex differences in IHD outcomes the prognosis of patients with premature IHD and reason for this is still unclear.

scholarly journals Features of gene polymorphism in comorbid patients with arterial hypertension and coronary heart disease

2021 ◽  
Vol 2 (4) ◽  
pp. 60-64
Author(s):  
L. V. Arutyunyan ◽  
N. V. Drobotya ◽  
A. A. Pirozhenko ◽  
V. V. Kaltukova
Keyword(s):  
Coronary Heart Disease ◽  
Heart Disease ◽  
High Risk ◽  
Gene Polymorphism ◽  
Ischemic Heart Disease ◽  
Arterial Hypertension ◽  
Molecular Genetic ◽  
Genetic Research ◽  
Ischemic Heart ◽  
Early Screening

Objective: to assess the prospects of using genetic testing for early detection of patients with arterial hypertension (AH) with a high risk of developing coronary heart disease (IHD).Material and methods: we examined 100 patients with hypertension, who were divided into 2 groups: patients with hypertension without ischemic heart disease (62%, 62 people) and patients with hypertension with ischemic heart disease (38%, 38 people). All patients underwent a standard volume of diagnostic procedures, as well as molecular genetic research. Results: the specificity of the carriage of gene polymorphism was revealed, depending on the presence of isolated AH or AH in combination with IHD in patients. Patients with AH and IHD are characterized by the presence of the CC genotype and the C allele of the ΝΟS3 gene (p = 0,040 and p = 0,035), while the TT genotype of the T‑786C polymorphic marker of the NOS3 gene is characteristic of patients with isolated AH. Conclusion: the study of the genetic aspects of comorbidity is theoretically important for understanding the mechanisms of its formation. From a practical point of view, the opportunity to use modern genetic approaches for early screening of hypertensive patients with a high risk of developing cardiac comorbid pathology is valuable.

scholarly journals Characteristics of miRNA interaction with 5'UTR, CDS and 3'UTR mRNA candidate genes of myocardial infarction and ischemic heart disease

2018 ◽  
Vol 76 (3) ◽  
pp. 62-82
Author(s):  
D. D. Baizhigitova ◽  
◽  
Sh. A. Atambayeva ◽  
R. E. Niyazova ◽  
A. T. Ivashchenko ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Heart Disease ◽  
Ischemic Heart Disease ◽  
Candidate Genes ◽  
Ischemic Heart

scholarly journals Serum miRNA Profile in Diabetic Patients with Ischemic Heart Disease as a Promising non-invasive Biomarker

2020 ◽  
Author(s):  
Agnieszka Bielska ◽  
Witold Bauer ◽  
Anna Szalkowska ◽  
Iwona Sidorkiewicz ◽  
Anna Skwarska ◽  
...  
Keyword(s):  
Heart Disease ◽  
Ischemic Heart Disease ◽  
Target Genes ◽  
Diagnostic Value ◽  
Functional Enrichment ◽  
Ischemic Heart ◽  
Individual Risk ◽  
Diabetic Patients ◽  
Serum Samples ◽  
Potential Biomarker

Abstract Background The increasing morbidity and mortality of type 2 diabetic mellitus (T2DM) patients with ischemic heart disease (IHD) highlights an urgent need to identify early biomarkers, which would help to predict individual risk of development of IHD. Here, we postulate that circulating serum-derived miRNAs may serve as potential biomarkers for early IHD diagnosis and help to identify diabetic individuals with a predisposition to undergo IHD. Methods We obtained serum samples from T2DM patients either with IHD or IHD-free and analysed the expression levels of 798 miRNAs using the NanoString nCounter Technology Platform. The prediction of the putative miRNAs targets was performed using the Ingenuity Pathway Analysis (IPA) software. Receiver operating characteristic (ROC) analysis was used to assess the diagnostic value of identified miRNAs. Results Our data showed that 9 miRNAs (miR-1224-5p, miR-1303, miR-3147, miR-4455, miR-498, miR-548b-3p, miR-548d-3p, miR-615-3p, miR-651-5p) were significantly upregulated in T2DM IHD patients compared to T2DM patients without IHD. In patients with upregulated miRNA, functional enrichment analysis of target genes by IPA indicated networks and canonical pathways involved in the pathology of the cardiovascular system. All tested miRNAs showed high diagnostic value (AUC > 0.8). Conclusions Taken together, our findings suggest that circulating miRNAs might have a crucial role in the development of IHD in diabetic patients and may be used as a potential biomarker for early diagnosis.

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