scholarly journals IDENTIFIKASI PATOGEN PENYEBAB BUSUK BATANG PADA BIBIT EUCALYPTUS PELLITA DI PERSEMAIAN

2018 ◽  
Vol 2 (1) ◽  
Author(s):  
Iin Arsensi

The pathogen attacks the host plant in various ways to obtain the food substance needed by the host pathogen. To get into the host pathogen is able to break the host's defense reaction. The aim of the study was to know the symptoms and signs of stem rot disease in E. pellita in the nursery. In addition, also to determine the frequency and intensity of rodent pathogen attack in plant seeds E. pellita from shoot cuttings. Research in the nursery begins by determining the sample of plant seeds to be observed. Seed determination is done by census. Seedlings consisted of 120 plants in a plastic rack that was repeated as many as 3 replications so that the total number of seedlings observed was 120 plant seed x 3 replicates x 4 families = 1.440 plant seeds. The pathogen that causes stem rot on E. pellita seed is Fusarium sp which is transmitted through soil.

2018 ◽  
Vol 2 (1) ◽  
Author(s):  
Iin Arsensi

The pathogen attacks the host plant in various ways to obtain the food substance needed by the host pathogen. To get into the host pathogen is able to break the host's defense reaction. The aim of the study was to know the symptoms and signs of stem rot disease in E. pellita in the nursery. In addition, also to determine the frequency and intensity of rodent pathogen attack in plant seeds E. pellita from shoot cuttings. Research in the nursery begins by determining the sample of plant seeds to be observed. Seed determination is done by census. Seedlings consisted of 120 plants in a plastic rack that was repeated as many as 3 replications so that the total number of seedlings observed was 120 plant seed x 3 replicates x 4 families = 1.440 plant seeds. The pathogen that causes stem rot on E. pellita seed is Fusarium sp which is transmitted through soil.


2019 ◽  
Vol 1 (1) ◽  
pp. 53
Author(s):  
Nur Adi

This study aims to determine the intensity of stem rot disease in dragon fruit plants in Samboja District Kutai Kartanegara Regency. This research was condu cted from November to December 2017 which is represented by four land i.e. two land located in Sungai Merdeka Village and two land located in Kelurahan Karya Merdeka. The method used in this research was interview and direct observation (survey). The sample was determined by purposive sampling or judgemental sampling method, that is method that chooses subject based on specified specific criteria (symptomatic plants) and sample of dragon fruits tems that attack disease in the field. The sample then isolated and identified the pathogen causing the disease at the Pest and Plant Disease Laboratory, Faculty of Agriculture Mulawarman University. The results of this study indicated that the percentage of stem rot disease in the District of Samboja KutaiKartanegara Regency of 60.83% with the severity of disease 36.12%. Results from the isolation of the ten drills of dragon fruit plants affected by stem rot disease identified the fungus Fusarium sp., Aspergillus sp. And Trichoderma sp.


Author(s):  
Weiguo Dong ◽  
Wenqing Ren ◽  
Xuan Wang ◽  
He Yuke

Abstract MicroRNA319a (miR319a) controls cell division arrest in plant leaves by inhibiting the expression of TCP (TEOSINTE BRANCHED 1/CYCLOIDEA/PCF) family genes. However, it is unclear whether miR319a influences infections by necrotrophic pathogens and host susceptibility. In this study, we revealed that miR319a affected plant resistance to stem rot disease of Sclerotinia sclerotiorum. In the plants of Brassica rapa infected with S. sclerotiorum, miR319a levels increased while expression levels of several BraTCP genes significantly decreased compared with those of the uninfected plants. The overexpression of BraMIR319a in B. rapa increased the susceptibility of the plants to S. sclerotiorum and aggravated stem rot disease, whereas the overexpression of BraTCP4-1 promoted the plant resistance. Our RNA-sequencing data revealed a potential relationship between miR319a and pathogen-related WRKY genes. Chromatin immunoprecipitation (ChIP) assay, electrophoretic mobility shift assay (EMSA) and reporter transaction assay showed that BraTCP4-1 was bound to the promoters of WRKY75, WRKY70, and WRKY33 genes and directly activated these pathogen-related genes. Moreover, the expression levels of WRKY75, WRKY70, and WRKY33 in the plants overexpressing BraMIR319a declined significantly whereas those of the plants overexpressing BraTCP4-1 increased significantly. These results suggest that miR319a and its targeted gene BraTCP4 regulate stem rot resistance through pathways of WRKY genes.


1999 ◽  
Vol 18 (2) ◽  
pp. 127-130 ◽  
Author(s):  
W.D. Branch ◽  
T.B. Brenneman
Keyword(s):  
Stem Rot ◽  

2014 ◽  
Vol 11 (10) ◽  
pp. 1841-1859 ◽  
Author(s):  
Fabien Fonguimgo Tengoua ◽  
Mohamed M. Hanafi ◽  
A. S. Idris ◽  
Kadir Jugah ◽  
Jamaludin Nurul Mayziatul Azwa ◽  
...  

2021 ◽  
Vol 17 (1) ◽  
pp. 35
Author(s):  
Wartono Wartono

<p>Chili (Capsicum annuum L.) is a vegetable commodity with high economic value which is widely cultivated by farmers in Indonesia. One of the obstacles faced in chili cultivation is stem rot disease. This study aimed to identify the pathogens that caused stem rot in chili plants obtained from one location in Sindangjaya Village, Cipanas District, Cianjur Regency, West Java Province based on morphological and molecular analyses. Pathogen identification was performed with morphological and molecular approaches. The morphological characters observed included colony shape, sporangium diameter, and mating type. The pathogenicity of the isolates was assayed by inoculating chili stems aged 40 days. Molecular identification was carried out using two pairs of primers for ITS regions and TEF-1 gene. Based on the results of morphological and molecular identification, as well as pathogenicity tests, it was confirmed that Phytophthora capsici pathogen was the causal agent of stem rot in chili plants collected from Sindangjaya Village. Further study is needed to determine the spread of the disease, damage, and yield loss caused by stem rot disease, as well as how to prevent and control the disease.</p>


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