scholarly journals Identification of Bacteria on Postcesarean Section Patient’s Wound Operation

2021 ◽  
pp. 38-41
Author(s):  
Renny Aditya ◽  
Risa Dirgagita
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Cesarean Section ◽  
Staphylococcus Epidermidis ◽  
Surgical Patient ◽  
Growth Media ◽  
Surgical Wound ◽  
Cross Sectional ◽  
Purposive Sampling ◽  
Wound Swab

Abstract Objective: To determine the type of bacteria in post-cesarean surgical patient wounds obtained through the patient's surgical wound swab in the Ward Obstetrics and Gynecology RSUD Ulin Banjarmasin in the period August-October 2019.Methods: This study was a descriptive study with a cross-sectional approach. A total of 36 samples were taken using a purposive sampling method, but only 32 bacterial isolates were obtained. Samples in the form of clean operating wound contaminated post-cesarean patients were planted on growth media and identified microscopically to be further classified based on responses to biochemical tests.Results: Descriptive analysis shows that there are three types of bacteria, namely Staphylococcus aureus as much as 59.3%, Staphylococcus epidermidis as much as 25.0%, and Escherichia coli as much as 15.6%.Conclusion: Obtained 3 types of bacteria in the results of surgical wound swab in post-cesarean section patients who were hospitalized in the Ward and Obstetrics Hospital of Ulin Hospital Banjarmasin, namely Staphylococcus aureus, Staphylococcus epidermidis, and Escherichia coli.Keywords: cesarean section, contaminant bacteria, surgery wound swab.   Abstrak Tujuan: Mengetahui gambaran jenis bakteri pada luka operasi pasien pascaseksio sesarea yang didapat melalui swab luka operasi pasien di Bangsal Kandungan dan Kebidanan RSUD Ulin Banjarmasin dalam periode Agustus-Oktober 2019.Metode: Penelitian ini merupakan studi dekskriptif dengan pendekatan potong lintang. Sebanyak 36 sampel diambil menggunakan metode purposive sampling, tetapi hanya didapatkan 32 isolat bakteri. Sampel swab luka operasi bersih terkontaminasi pasien paska seksio sesarea ditanam pada media pertumbuhan dan diidentifikasi secara mikroskopis untuk selanjutnya diklasifikasikan berdasarkan respon terhadap uji biokimia.Hasil : Analisis secara deksriptif menunjukkan bahwa terdapat tiga jenis bakteri, yaitu Staphylococcus aureus sebanyak 59,3%, Staphylococcus epidermidis sebanyak 25,0%, dan Escherichia coli sebanyak 15,6%.Kesimpulan : Didapatkan 3 jenis bakteri pada hasil swab luka operasi pasien pascaseksio sesarea yang dirawat inap di Bangsal Kandungan dan Kebidanan RSUD Ulin Banjarmasin, yaitu Staphylococcus aureus, Staphylococcus epidermidis, dan Escherichia coli.Kata kunci: seksio sesarea, bakteri kontaminan, swab luka operasi.

scholarly journals Bacteria Sensitivity Pattern of Caesarean Section Wound on Selected Antibiotics in Ulin General Hospital Banjarmasin

2020 ◽  
Vol 16 (2) ◽  
pp. 73
Author(s):  
Nurhafizah Rafiani ◽  
Renny Aditya ◽  
Noor Muthmainah
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Caesarean Section ◽  
Staphylococcus Epidermidis ◽  
Antibiotic Sensitivity ◽  
Cross Sectional ◽  
Surgical Wounds ◽  
Sensitivity Pattern ◽  
Antibiotic Sensitivity Test ◽  
Bacterial Sensitivity

Abstract: A cesarean delivery increases the risk of wound infection it should be prevented by using antibiotics. This study was to determine the pattern of bacterial sensitivity in surgical wounds of cesarean section patients for selected antibiotics, i.e ceftriaxone, cefixime, sulbactam ampicillin, ciprofloxacin, clindamycin and gentamicin.This observational study was conducted at the Ulin Public Hospital in Banjarmasin from July to September 2019. Using a cross sectional approach Samples of bacteria were identidied from 36 that were pusposively sampled, i.e., Staphylococcus aureus, Staphylococcus epidermidis and Escherichia coli. Antibiotic sensitivity test showed that Staphylococcus aureus was sensitive against gentamicin (100%),whereas Staphylococcus epidermidis and Escherichia coli. Were intermediately sensitive towards gentamicin (62.5%) and ceftriaxone (80%) Keywords: Antibiotic susceptibility, caesarean section, surgical wound

scholarly journals Análise Microbiológica em Aparelhos de Celular de Acadêmicos e Professores da Universidade de Cuiabá (UNIC) Campus Primavera do Leste – MT

UNICIÊNCIAS ◽  
2019 ◽  
Vol 23 (2) ◽  
pp. 105
Author(s):  
Vivian Tallita Pinheiro de Santana ◽  
Phelipe Magalhães Duarte ◽  
Uvleique Alves Fernandes ◽  
Gabriel Moreno Damião ◽  
Amanda Lourença Da Silva
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Staphylococcus Epidermidis ◽  
Cell Phones ◽  
Control Measures ◽  
Growth Media ◽  
Staphylococcus Lugdunensis ◽  
Nutrient Culture ◽  
Microbiological Agents ◽  
Staphylococcus Spp

Rápidos, portáteis e acessíveis, os aparelhos celulares foram incorporados às atividades e rotinas diárias da população. Diante de tal intimidade, esses aparelhos também passaram a se constituir em um meio de crescimento para agentes microbiológicos comensais e acidentais. Medidas de controle como a adoção de boas práticas de higiene são imprescindíveis para a diminuição da carga microbiológica e, por consequência, decréscimo dos riscos de transmissão destes agentes ao homem, principalmente em imunodeprimidos. Dessa forma, o presente trabalho objetivou identificar os micro-organismos presentes em aparelhos celulares de acadêmicos e professores da Universidade de Cuiabá (UNIC), Campus Primavera do Leste – MT, através de amostragens obtidas de 24 aparelhos de telefones celulares de voluntários. Foram realizadas coletas para cada um dos aparelhos celulares disponibilizados pelos voluntários, através de um swab de transporte. Este foi deslizado na parte externa do aparelho, cobrindo toda a área da tela, conectores e alto-falante e, em seguida, inseridos em meio Stuart e remetidos ao laboratório. Do total de amostras coletadas, 19 (79,1%) apresentaram crescimento bacteriano em meio de cultura nutritivo. Os micro-organismos isolados foram Staphylococcus aureus em seis amostras (31,6%), Staphylococcus lugdunensis em outras seis (31,6%), Staphylococcus epidermidis em três (15,8%), Staphylococcus spp. em três (5,8%) e Escherichia coli em uma (5,2%) das amostras. Diante dos resultados obtidos se pôde constatar que os aparelhos celulares podem atuar como meio de multiplicação microbiológica, assim se deve adotar medidas preventivas de higiene e antissepsia das mãos e destes aparelhos, a fim de evitar a proliferação e veiculação de micro-organismos por meio destes. Palavras-chave: Telefone Celular. Staphylococcus aureus.  Escherichia coli. AbstractFast, portable and affordable, mobile phones have been incorporated into the daily activities and routines of the population. Faced with such intimacy, these devices have also become a growth medium for commensal and accidental microbiological agents. Control measures such as the adoption of good hygiene practices are essential to reduce the microbiological load and, consequently, decrease the risks of transmission of these agents to humans, especially in immunosuppressed ones. Thus, the present work aimed to identify the microorganisms present in cell phones of academics and professors of the University of Cuiabá (UNIC), Campus Primavera do Leste - MT, through samples obtained from 24 cell phones of volunteers. The samples  were collected for each of the mobile devices made available by the volunteers through a transport swab. This was slid on the external part of the device, covering the entire display area, connectors and speaker, then inserted into Stuart medium and sent to the lab. Of the total samples collected, only 19 (79.1%) showed bacterial growth in nutrient culture medium. Microorganisms isolated from growth media were Staphylococcus aureus in six samples (31.6%), Staphylococcus lugdunensis in six (31.6%), Staphylococcus epidermidis in three (15.8%), Staphylococcus spp. in three (5.8%) and Escherichia coli in one (5.2%) of the samples. Given the results obtained, it can be seen that cell phones can act as a means of microbiological multiplication, so preventive measures of hygiene and antisepsis of  hands should be adopted, in order to prevent the proliferation and spread of microorganisms through them . Keyword: Cell Phone. Staphylococcus aureus.  Escherichia coli. 

scholarly journals UJI SENSITIVITAS BAKTERI PENYEBAB INFEKSI SALURAN KEMIH PADA PASIEN DIABETES MELITUS TERHADAP SEFTRIAKSON, LEVOFLOKSASIN, DAN GENTAMISIN

2016 ◽  
Vol 12 (2) ◽  
pp. 205
Author(s):  
Nova Octavianty Rachman ◽  
Muhamad Darwin Prenggono ◽  
Lia Yuli Budiarti
Keyword(s):  
Diabetes Mellitus ◽  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Staphylococcus Epidermidis ◽  
Sensitivity Test ◽  
Cross Sectional ◽  
Diabetes Melitus

Abstract: Urinary Tract Infection (UTI) is a general term that indicates the presences or propagation of microorganism in urine. The urinary tract infection is a very common condition that occurs in both women and men of all ages. Diabetes Mellitus (DM) can lead to the development of bacteriuria. Bacterial resistance to antibiotics prolongs the UTI recover. The aim of this research was to find out bacterial sensitivity urinary tract infection in patients diabetes mellitus to selected antibiotic during July-September 2015. It was a descriptional study with cross-sectional. The subject of this research is all patients diabetes mellitus with urinary tract infection in internal medicine wards Ulin General Hospital Banjarmasin. The sampling technique in this research was a total sampling. The sensitivity test to antibiotic test in the research used Kirby Bauer method and analyzed according to CLSI 2013 standard.This type of bacteria found in this research are Escherichia coli, Pseudomonas aerugenosa, Staphylococcus epidermidis, and Staphylococcus aureus. The sensitivity test result the percentage type of bacteria are sensitive to ceftriaxone 73%, levofloxaxin 84,6%, and gentamicin 73%. The conclution is that most sensitive antibiotic is levofloxaxin (84,6%) Keywords: diabetes mellitus (DM), urinary tract infection (UTI), ceftriaxone, levofloxaxin, gentamicin. Abstrak: Infeksi Saluran Kemih (ISK) didefinisikan sebagai adanya infeksi bakteri dalam urin yang ditandai dengan bakteriuria bermakna. Infeksi saluran kemih merupakan kondisi yang sangat umum terjadi baik pada wanita maupun pria pada semua usia. Diabetes Melitus (DM) dapat menyebabkan perkembangan bakteriuria. Resistensi bakteri terhadap antibiotik menyebabkan lamanya penyembuhan dari ISK. Penelitian ini bertujuan mengetahui sensitivitas bakteri penyebab ISK pada pasien DM terhadap antibiotik terpilih periode Juli-September 2015. Penelitian ini merupakan penelitian deskriptif dengan pendekatan Cross Sectional. Subjek penelitian ini adalah seluruh pasien DM dengan ISK Rawat Inap di Bangsal Penyakit Dalam RSUD Ulin Banjarmasin. Teknik pengambilan sampel pada penelitian ini adalah Total Sampling. Uji sensitivitas bakteriuria terhadap antibiotik uji pada penelitian ini menggunakan metode Kirby-Bauer dan dianalisis secara deskriptif sesuai dengan standar CLSI 2013. Jenis bakteri yang ditemukan pada penelitian ini adalah Escherichia coli, Pseudomonas aerugenosa, Staphylococcus epidermidis, Staphylococcus aureus. Hasil uji sensitivitas menunjukkan bahwa presentase jenis bakteri yang sensitif terhadap Seftriakson 73%, Levofloksasin 84,6%, dan Gentamisin 73%. Persentase isolat bakteri yang resisten terhadap Seftriakson 15,3%, Levofloksasin 3,8%, dan Gentamisin 3,8%. Kesimpulan antibiotik yang paling sensitif terhadap bakteri penyebab ISK pada penilitian ini adalah Levofloksasin (84,6%). Kata-Kata kunci: diabetes melitus (DM), infeksi saluran kemih (ISK), seftriaxon, levofloksasin, gentamisin

scholarly journals Epidemiology and Microbiology of Surgical Wound Infections

2000 ◽  
Vol 38 (2) ◽  
pp. 918-922 ◽  
Author(s):  
A. Giacometti ◽  
O. Cirioni ◽  
A. M. Schimizzi ◽  
M. S. Del Prete ◽  
F. Barchiesi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Staphylococcus Epidermidis ◽  
Signs And Symptoms ◽  
Etiologic Agent ◽  
Aerobic Bacteria ◽  
Wound Infections ◽  
Surgical Wound ◽  
The Common

This study included 676 surgery patients with signs and symptoms indicative of wound infections, who presented over the course of 6 years. Bacterial pathogens were isolated from 614 individuals. A single etiologic agent was identified in 271 patients, multiple agents were found in 343, and no agent was identified in 62. A high preponderance of aerobic bacteria was observed. Among the common pathogens wereStaphylococcus aureus (191 patients, 28.2%),Pseudomonas aeruginosa (170 patients, 25.2%),Escherichia coli (53 patients, 7.8%), Staphylococcus epidermidis (48 patients, 7.1%), and Enterococcus faecalis (38 patients, 5.6%).

scholarly journals Atividade antibacteriana de extratos de folhas de Montrichardia linifera (Arruda) Schott (Araceae)

2015 ◽  
Vol 17 (4 suppl 3) ◽  
pp. 1142-1149 ◽  
Author(s):  
J.A.L MIRANDA ◽  
J.A. ROCHA ◽  
K.M. ARAÚJO ◽  
P.V. QUELEMES ◽  
S.J. MAYO ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Klebsiella Pneumoniae ◽  
Enterococcus Faecalis ◽  
Staphylococcus Epidermidis

RESUMO O uso de plantas medicinais no tratamento de doenças é uma estratégia antiga utilizada por praticamente todas as populações do mundo, e, embora novos antibióticos tenham sido desenvolvidos para o controle de micro-organismos infecciosos, às vezes são ineficazes. Diversos extratos de plantas medicinais têm efeitos antimicrobianos, principalmente quando associados à antibióticos de uso clínico, representando alternativa terapêutica para doenças infecciosas. Montrichardia linifera, conhecida popularmente como aninga, é espécie macrófita, aquática emergente de hábito herbáceo, pertencente a família Araceae e ocorre em áreas alagáveis. A utilidade farmacológica desta espécie é diversificada tendo sido relatada como cicatrizante, antirreumático, antidiurético e expectorante. Devido à relevância no campo etnofarmacológico, ampla utilização na medicina popular e escassez de trabalhos relacionados à atividade antibacteriana desta espécie, objetivou-se com este trabalho avaliar a atividade antibacteriana de extratos alcoólicos de folhas de Montrichardia linifera, coletadas na margem do rio Igaraçu, Parnaíba-PI. O extrato foi testado em oito cepas de bactérias: Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis, Escherichia coli, Klebsiella pneumoniae e Pseudomonas aeruginosa. Foram utilizadas as técnicas de verificação da formação de halos de inibição e determinação das concentrações inibitórias e bactericidas mínimas. Os testes antibacterianos evidenciaram como principais resultados que o extrato metanólico seco (EMS), extrato metanólico fresco (EMF), e o extrato etanólico seco (EES), apresentaram ação antibacteriana, enquanto o extrato etanólico fresco (EEF) não apresentou atividade para as bactérias testadas. O EMS foi o mais eficiente, inibindo o crescimento bacteriano na concentração de 200 μg/mL para E. faecalis, 400 μg/mL para S. aureus, 400 μg/mL para S. epidermidis e 2.000 μg/mL para P. aeruginosa. O EMF obteve CIM de 2.000 μg/mL para E. faecalis e EES obteve CIM de 250 μg/mL para E. faecalis. Os resultados demonstraram que M. linifera constitui fonte eficiente de compostos bioativos antibacterianos. Os estudos sobre as propriedades farmacológicas de plantas da família Araceae são escassos, e os resultados deste trabalho são pioneiros em relação a atividade antibacteriana desta espécie.

scholarly journals Perfil microbiológico y resistencia antibiótica en el servicio de pediatría de una clínica privada, 2016

2019 ◽  
Vol 12 (2) ◽  
pp. 159-165
Author(s):  
Francheska Coricaza-Cuaresma ◽  
Moisés Apolaya-Segura ◽  
Cristian Díaz-Vélez
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Staphylococcus Epidermidis ◽  
E Coli

Objetivo: Describir el perfil microbiológico y de resistencia antibiótica en el servicio de pediatría de la Clínica Jesús del Norte, 2016. Material y métodos: Se diseñó un estudio descriptivo de serie de casos. El estudio se desarrolló en el Servicio de Pediatría de la Clínica Jesús del Norte. Resultados: Se analizaron 1264 muestras de pacienres <15 años con cultivos positivos de orina, heces, sangre y secreción faríngea. El 67,7% fue en <5 años y predomino en mujeres con 59,22%. El germen aislado en mayor frecuencia fue Escherichia coli con 46,68%. Por tipo de muestra el más frecuente fueron: En Coprocultivo: Escherichia coli enteropatógena, Urocultivo: Escherichia coli, Hemocultivo: Staphylococcus epidermidis y en secreción faríngea: Staphylococcus aureus. Llamó la atención la presencia de 105 casos (7,27%) de E. coli BLEE en urocultivos. Conclusiones: Los cultivos positivos fueron más frecuentes en mujeres, en menores de 5 años, atendidos en emergencia y de muestra de heces. Apreciándose alta frecuencia de resistencia a fármacos de primera y segunda línea en diversos agentes etiológicos aislados en las muestras.

scholarly journals Νέες τεχνικές ανάλυσης ειδικών αλληλουχιών DNA και RNA

2006 ◽  
Author(s):  
Δέσποινα Καλογιάννη
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Streptococcus Pneumoniae ◽  
Staphylococcus Epidermidis ◽  
Rt Pcr ◽  
Haemophilus Influenza

Οι μέθοδοι ανίχνευσης και προσδιορισμού ειδικών αλληλουχιών νουκλεϊκών οξέων βασίζονται στην υβριδοποίηση (μοριακή αναγνώριση) του αναλύτη με συμπληρωματικό μόριο DNA/RNA, το οποίο είναι συνδεδεμένο, άμεσα ή έμμεσα, με κατάλληλο ιχνηθέτη για παραγωγή σήματος. Το φάσμα εφαρμογών των μεθόδων υβριδοποίησης συνεχώς διευρύνεται, κυρίως ως αποτέλεσμα της προόδου των προγραμμάτων προσδιορισμού της αλληλουχίας του γονιδιώματος του ανθρώπου και άλλων οργανισμών. Σκοπός της παρούσας διατριβής είναι αφενός η ανάπτυξη μεθόδων υβριδοποίησης που παρέχουν τη δυνατότητα ταυτόχρονου προσδιορισμού πολλών αλληλουχιών νουκλεϊκών οξέων και αφετέρου η απλοποίηση των μεθόδων υβριδοποίησης με τη χρήση βιοαισθητήρα DNA. Στο Κεφάλαιο 1 της διατριβής περιγράφεται η αρχή της αλυσιδωτής αντίδρασης πολυμεράσης (PCR) και η αντίστροφη μεταγραφή-PCR (RT-PCR) ως οι πιο σημαντικές τεχνικές εκθετικού πολλαπλασιασμού ειδικών αλληλουχιών DNA και RNA, οι οποίες αποτελούν αναπόσπαστο μέρος κάθε σύγχρονης μεθόδου ανάλυσης νουκλεϊκών οξέων. Παρουσιάζεται επίσης η τεχνική της ποσοτικής PCR και διάφορες μέθοδοι προσδιορισμού των προϊόντων της PCR. Στο Κεφάλαιο 2 γίνεται παρουσίαση των δοκιμασιών υβριδοποίησης νουκλεϊκών οξέων, οι οποίες αποτελούν και τον ακρογωνιαίο λίθο της αναλυτικής χημείας του DNA και RNA. Περιγράφεται η εξέλιξη των ιχνηθετών από τα ραδιοϊσότοπα μέχρι τους πιο σύγχρονους μη ραδιενεργούς ιχνηθέτες που παρέχουν υψηλή ανιχνευσιμότητα και δυνατότητες αυτοματοποίησης. Παρουσιάζονται οι ομογενείς και ετερογενείς δοκιμασίες υβριδοποίησης και διάφορες παραλλαγές που διευκολύνουν την εκτέλεση τους. Στο Κεφάλαιο 3 παρέχεται σύντομη περιγραφή της αρχής λειτουργίας και της οργανολογίας της κυτταρομετρίας ροής, η οποία αποτελεί ένα ισχυρό αναλυτικό εργαλείο για τη διερεύνηση ιδιοτήτων μικροσωματιδίων (π.χ. κυττάρων αλλά και συνθετικών μικροσφαιριδίων). Αντικείμενο του Κεφαλαίου 4 είναι η ανάπτυξη πολλαπλών φθορισμομετρικών δοκιμασιών υβριδοποίησης DNA και RNA σε εναιώρημα φασματικά διακριτών μικροσφαιριδίων πολυστυρενίου. Κάθε μικροσφαιρίδιο περιέχει δύο φθορίζουσες χρωστικές σε συγκεκριμένη αναλογία και με αυτό τον τρόπο έχει καταστεί ένα φασματικά κωδικοποιημένο στερεό υπόστρωμα για την εκτέλεση δοκιμασιών υβριδοποίησης. Ειδικά ολιγονουκλεοτίδια-ανιχνευτές προσδένονται ομοιοπολικά στην επιφάνεια κάθε μικροσφαιριδίου. Η αλληλουχία του DNA- ή RNA-στόχου (αναλύτης) υβριδοποιείται με τα ακινητοποιημένα ολιγονουκλεοτίδια και τα υβρίδια ποσοτικοποιούνται με άλλη φθορίζουσα ουσία. Το εναιώρημα αναλύεται με κυτταρομετρητή ροής με ανιχνευτή φθορισμού επαγώμενου με δύο πηγές λέιζερ. Μελετήθηκε ο προσδιορισμός μονόκλωνου DNA, δίκλωνου DNA και RNA και πραγματοποιήθηκαν πειράματα ανάλυσης γονιδιακής έκφρασης σε ασθενείς με λευχαιμία. Στο Κεφάλαιο 5 παρουσιάζεται ο πρώτος βιοαισθητήρας DNA τύπου εμβαπτιζόμενης ταινίας ξηρών αντιδραστηρίων για την ανίχνευση 7 χρωμοσωμικών αλληλομεταθέσεων, οι οποίες σχετίζονται άμεσα με την οξεία ή τη χρόνια λευχαιμία. Ο βιοαισθητήρας βασίζεται σε ολιγονουκλεοτίδια συζευγμένα με νανοσωματίδια χρυσού και επιτρέπει την ταυτοποίηση των αλληλομεταθέσεων δια γυμνού οφθαλμού, έπειτα από πολλαπλασιασμό με RT-PCR. Σε αντίθεση με τις μέχρι τώρα χρησιμοποιούμενες μεθόδους ανίχνευσης αλληλομεταθέσεων, η ανάλυση με το βιοαισθητήρα είναι απλή, δεν απαιτεί ειδικά όργανα και αποφεύγει πολλά στάδια επωάσεων και εκπλύσεων. Αντικείμενο του Κεφαλαίου 6 της διατριβής είναι η ανάπτυξη μεθόδου υβριδοποίησης DNA για την ταχεία ανίχνευση βακτηριακής επιμόλυνσης σε αρθροπλαστικές επεμβάσεις, ένα σημαντικό πρόβλημα στην ορθοπεδική. Απομονώνεται DNA από δείγματα αρθρικού υγρού, ακολουθεί πολλαπλασιασμός με PCR χρησιμοποιώντας καθολικούς εκκινητές και ανίχνευση των προϊόντων με βιοαισθητήρα DNA. Ανιχνεύονται οι μικροοργανισμοί Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis, Haemophilus influenza, Enterococcus faesium και Streptococcus pneumoniae. Στο Κεφάλαιο 7 περιγράφεται η εφαρμογή του βιοαισθητήρα DNA για την ανίχνευση γενετικά τροποποιημένων οργανισμών (GMO) σε τρόφιμα. Αν και η παρουσία GMO μπορεί να διαπιστωθεί με ανίχνευση είτε της νέας πρωτεΐνης που συνθέτει το διαγονιδιακό φυτό ή της αλληλουχίας DNA που έχει εισαχθεί σ’ αυτό, το DNA είναι ο προτιμότερος αναλύτης λόγω της μεγαλύτερης σταθερότητάς του, ιδιαίτερα σε επεξεργασμένα τρόφιμα. Χαρακτηριστικές αλληλουχίες DNA που περιέχονται στα περισσότερα GMO, δηλαδή ο προαγωγέας 35S και ο τερματιστής NOS, πολλαπλασιάζονται με PCR και τα προϊόντα ανιχνεύονται με δοκιμασία υβριδοποίησης στο βιοαισθητήρα DNA. Η παρούσα εργασία εισάγει, για πρώτη φορά στο πεδίο της ανάλυσης GMO, έναν βιοαισθητήρα DNA τύπου εμβαπτιζόμενης ταινίας ξηρών αντιδραστηρίων.

scholarly journals Combination Effects of Cymbopogon sp. Essential Oil on Selected Bacteria

2018 ◽  
Vol 1 (1) ◽  
Author(s):  
Nur Aishah Abdul Wahab ◽  
Hairul Shahril Muhamad ◽  
Nabilah Ahmad Alhadi ◽  
Salina Mat Radzi ◽  
Maryam Mohamed Rehan ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Bacillus Subtilis ◽  
Essential Oils ◽  
Staphylococcus Epidermidis ◽  
Antimicrobial Agents ◽  
Synergistic Effects ◽  
Combination Effects ◽  
Cymbopogon Flexuosus ◽  
Combination Study

Combination effects between Cymbopogon flexuosus and Cymbopogon nardus essential oils were studied to determine whether the combination could emerge as better and more powerful antimicrobial agents against six selected bacteria includes Bacillus subtilis, Escherichia coli, Salmonella typhimurium, Serratia marcescens, Staphylococcus aureus, and Staphylococcus epidermidis. This combination study exhibited 40.67% additive, 28.67% antagonistic, 16.00% indifferent and 14.66% synergistic effects. C. flexuosus and C. nardus essential oils in combination showed a high inhibitory activity against S. aureus with 16% synergistic, 64% additive and 20% indifferent effects.

scholarly journals ESTUDO FITOQUÍMICO E DE ATIVIDADE ANTIMICROBIANA DE Ptychopetalum olacoides Bentham

2002 ◽  
Vol 3 (2) ◽  
Author(s):  
P. D. MONTRUCCHIO ◽  
O. G. MIGUEL
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Fusarium Oxysporum ◽  
Staphylococcus Epidermidis ◽  
Antimicrobial Properties ◽  
Colletotrichum Acutatum ◽  
Alcoholic Extract ◽  
Phytochemical Study ◽  
North Brazil ◽  
Long Time

A espécie Ptychopetalum olacoides Bentham, popularmente conhecida como “marapuama” ou “muirapuama”, é uma Olacaceae nativa da região norte do Brasil, há muito conhecida e utilizada por suas propriedades estimulantes e afrodisíacas, sendo inclusive exportada para diversos países. O estudo fitoquímico do lenho desta árvore, neste trabalho representado pelos galhos da planta, revelou a presença majoritária de vários ácidos graxos, esteróides e xantinas, sendo eles: ácido palmítico, ácido esteárico, -sitosterol, estigmasterol, lupeol, glutinol, a-amirina, cafeína, teobromina e adenina, sendo que as três últimas não haviam ainda sido reportadas na espécie. O estudo das atividades antimicrobianas revelou que os extratos da planta não apresentam atividade inibitória sobre o desenvolvimento de cepas de Escherichia coli, Staphylococcus aureus ou Staphylococcus epidermidis. Porém, o resíduo do extrato alcoólico apresenta uma significativa ação inibitória do crescimento micelial de Colletotrichum acutatum, e ação menos pronunciada sobre o crescimento de Fusarium oxysporum. PHYTOCHEMICAL STUDY AND ANTIMICROBIAL ACTIVITY OF Ptychopetalumolacoides Bentham Abstract Ptychopetalum olacoides Bentham, popularly known as “marapuama” or “muirapuama”, is an Olacaceae native from north Brazil, known and used for its stimulating and aphrodisiac properties for a long time, being also exported to several countries all over the world. The phytochemical study of the wood of this tree, represented by its stems, revealed the presence of several fatty acids, sterols and xanthines, including palmitic acid, stearic acid, -sitosterol, stigmasterol, lupeol, glutinol, a-amirin, caffeine, theobromine and adenine, and the latest three substances have not been reported in this specie yet. The study of antimicrobial properties revealed that the plant extracts don’t have any inhibitory activity against strains of Escherichia coli, Staphylococcus aureus or Staphylococcus epidermidis. However, the alcoholic extract residue does have a remarkable inhibitory action on the mycelial growth of Colletotrichum acutatum and Fusarium oxysporum.26

scholarly journals Actividad antibacteriana in vitro de aceites esenciales de diferentes especies del género Citrus

2017 ◽  
Vol 46 (2) ◽  
Author(s):  
Miladys Esther Torrenegra Alarcón ◽  
Nerlis Paola Pájaro ◽  
Glicerio León Méndez
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Klebsiella Pneumoniae ◽  
Staphylococcus Epidermidis ◽  
E Coli

Se evaluó la actividad antibacteriana in vitro de aceites esenciales de diferentes especiesdel género Citrus frente a cepas ATCC de Staphylococcus aureus, Staphylococcus epidermidis,Klebsiella pneumoniae, Pseudomonas aeruginosa y Escherichia coli, determinandola concentración mínima inhibitoria (CMI) y la concentración mínima bactericida(CMB). Las bacterias se replicaron en medios de agar y caldos específicos. Se determinóel momento de máxima densidad óptica (DO620) para emplearlo como tiempode incubación; luego se hicieron pruebas de evaluación de sensibilidad con la exposiciónde las cepas a concentraciones a 1000 g/mL del extracto en caldo. Para solubilizarse empleó dimetilsulfóxido (DMSO) al 1%. Posteriormente, se le determinó laconcentración mínima inhibitoria mediante metodologías de microdilución en caldoy la concentración mínima bactericida. Encontrándose una actividad de los aceitesesenciales del género Citrus, con valores de CMI ≥ 600 mg/mL frente a S. aureus,S. epidermidis, K. pneumoniae, P. aeruginosa y E. coli. En función a los resultados obtenidos,se concluye que las diferentes especies del género Citrus son consideradas comopromisorias para el control del componente bacteriano.

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