scholarly journals Análise Microbiológica em Aparelhos de Celular de Acadêmicos e Professores da Universidade de Cuiabá (UNIC) Campus Primavera do Leste – MT

UNICIÊNCIAS ◽  
2019 ◽  
Vol 23 (2) ◽  
pp. 105
Author(s):  
Vivian Tallita Pinheiro de Santana ◽  
Phelipe Magalhães Duarte ◽  
Uvleique Alves Fernandes ◽  
Gabriel Moreno Damião ◽  
Amanda Lourença Da Silva
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Staphylococcus Epidermidis ◽  
Cell Phones ◽  
Control Measures ◽  
Growth Media ◽  
Staphylococcus Lugdunensis ◽  
Nutrient Culture ◽  
Microbiological Agents ◽  
Staphylococcus Spp

Rápidos, portáteis e acessíveis, os aparelhos celulares foram incorporados às atividades e rotinas diárias da população. Diante de tal intimidade, esses aparelhos também passaram a se constituir em um meio de crescimento para agentes microbiológicos comensais e acidentais. Medidas de controle como a adoção de boas práticas de higiene são imprescindíveis para a diminuição da carga microbiológica e, por consequência, decréscimo dos riscos de transmissão destes agentes ao homem, principalmente em imunodeprimidos. Dessa forma, o presente trabalho objetivou identificar os micro-organismos presentes em aparelhos celulares de acadêmicos e professores da Universidade de Cuiabá (UNIC), Campus Primavera do Leste – MT, através de amostragens obtidas de 24 aparelhos de telefones celulares de voluntários. Foram realizadas coletas para cada um dos aparelhos celulares disponibilizados pelos voluntários, através de um swab de transporte. Este foi deslizado na parte externa do aparelho, cobrindo toda a área da tela, conectores e alto-falante e, em seguida, inseridos em meio Stuart e remetidos ao laboratório. Do total de amostras coletadas, 19 (79,1%) apresentaram crescimento bacteriano em meio de cultura nutritivo. Os micro-organismos isolados foram Staphylococcus aureus em seis amostras (31,6%), Staphylococcus lugdunensis em outras seis (31,6%), Staphylococcus epidermidis em três (15,8%), Staphylococcus spp. em três (5,8%) e Escherichia coli em uma (5,2%) das amostras. Diante dos resultados obtidos se pôde constatar que os aparelhos celulares podem atuar como meio de multiplicação microbiológica, assim se deve adotar medidas preventivas de higiene e antissepsia das mãos e destes aparelhos, a fim de evitar a proliferação e veiculação de micro-organismos por meio destes. Palavras-chave: Telefone Celular. Staphylococcus aureus.  Escherichia coli. AbstractFast, portable and affordable, mobile phones have been incorporated into the daily activities and routines of the population. Faced with such intimacy, these devices have also become a growth medium for commensal and accidental microbiological agents. Control measures such as the adoption of good hygiene practices are essential to reduce the microbiological load and, consequently, decrease the risks of transmission of these agents to humans, especially in immunosuppressed ones. Thus, the present work aimed to identify the microorganisms present in cell phones of academics and professors of the University of Cuiabá (UNIC), Campus Primavera do Leste - MT, through samples obtained from 24 cell phones of volunteers. The samples  were collected for each of the mobile devices made available by the volunteers through a transport swab. This was slid on the external part of the device, covering the entire display area, connectors and speaker, then inserted into Stuart medium and sent to the lab. Of the total samples collected, only 19 (79.1%) showed bacterial growth in nutrient culture medium. Microorganisms isolated from growth media were Staphylococcus aureus in six samples (31.6%), Staphylococcus lugdunensis in six (31.6%), Staphylococcus epidermidis in three (15.8%), Staphylococcus spp. in three (5.8%) and Escherichia coli in one (5.2%) of the samples. Given the results obtained, it can be seen that cell phones can act as a means of microbiological multiplication, so preventive measures of hygiene and antisepsis of  hands should be adopted, in order to prevent the proliferation and spread of microorganisms through them . Keyword: Cell Phone. Staphylococcus aureus.  Escherichia coli. 

scholarly journals Detección fenotípica de susceptibilidad a meticilina, eritromicina y clindamicina en aislados de Staphylococcus spp. de un hospital de Valledupar (Colombia)

2017 ◽  
Vol 23 (1-2) ◽  
pp. 65-74
Author(s):  
Gloria I. Morales-Parra ◽  
María C. Yaneth-Giovanetti ◽  
Andrés B. Zuleta-Hernández ◽  
Marilyn L. Núñez-Carrillo
Keyword(s):  
Staphylococcus Aureus ◽  
Staphylococcus Epidermidis ◽  
Staphylococcus Lugdunensis ◽  
Staphylococcus Saprophyticus ◽  
Staphylococcus Haemolyticus ◽  
Staphylococcus Hominis ◽  
Staphylococcus Spp

Introducción: las infecciones por Staphylococcus spp. multirresistentes están asociadas con una mayor morbimortalidad de los pacientes afectados. Objetivo: caracterizar fenotipos de resistencia a meticilina, macrólidos y lincosamidas de 50 aislados de Staphylococcus spp. provenientes de pacientes de un centro hospitalario en la ciudad de Valledupar (Colombia). Materiales y métodos: las pruebas de susceptibilidad a meticilina, eritromicina y clindamicina se realizaron por los métodos de microdilución en caldo y difusión en agar. La resistencia a meticilina se tamizó por la técnica de dilución en agar y la resistencia inducible a clindamicina por la prueba D. Resultados: los aislados de Staphylococcus fueron obtenidos principalmente de heridas (58%) y orinas (12%) y en las áreas de consulta externa (40%), cirugía (24%) y urgencias (10%). Staphylococcus aureus se aisló en un 68%, seguido de Staphylococcus epidermidis (14%), Staphylococcus lugdunensis (8%), Staphylococcus saprophyticus (4%), Staphylococcus haemolyticus (4%) y Staphylococcus hominis (2%). La resistencia a meticilina se encontró en el 36% de los aislados de Staphylococcus aureus y el 8% de los estafilococos coagulasa negativos. Se evidenciaron cinco fenotipos de resistencia; el fenotipo con sensibilidad a eritromicina y clindamicina fue el más frecuente (54%), seguido del de resistencia a ambos antibióticos (14%). La resistencia inducible a clindamicina fue del 12%, encontrándose en el 8% de aislados de Staphylococcus aureus y el 4% de los de Staphylococcus epidermidis. Conclusiones: la prueba D es esencial para detectar el fenotipo de resistencia inducible a clindamicina en aislados de Staphylococcus spp. y evitar su administración frente al inminente fracaso terapéutico.

scholarly journals Identification of Bacteria on Postcesarean Section Patient’s Wound Operation

2021 ◽  
pp. 38-41
Author(s):  
Renny Aditya ◽  
Risa Dirgagita
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Cesarean Section ◽  
Staphylococcus Epidermidis ◽  
Surgical Patient ◽  
Growth Media ◽  
Surgical Wound ◽  
Cross Sectional ◽  
Purposive Sampling ◽  
Wound Swab

Abstract Objective: To determine the type of bacteria in post-cesarean surgical patient wounds obtained through the patient's surgical wound swab in the Ward Obstetrics and Gynecology RSUD Ulin Banjarmasin in the period August-October 2019.Methods: This study was a descriptive study with a cross-sectional approach. A total of 36 samples were taken using a purposive sampling method, but only 32 bacterial isolates were obtained. Samples in the form of clean operating wound contaminated post-cesarean patients were planted on growth media and identified microscopically to be further classified based on responses to biochemical tests.Results: Descriptive analysis shows that there are three types of bacteria, namely Staphylococcus aureus as much as 59.3%, Staphylococcus epidermidis as much as 25.0%, and Escherichia coli as much as 15.6%.Conclusion: Obtained 3 types of bacteria in the results of surgical wound swab in post-cesarean section patients who were hospitalized in the Ward and Obstetrics Hospital of Ulin Hospital Banjarmasin, namely Staphylococcus aureus, Staphylococcus epidermidis, and Escherichia coli.Keywords: cesarean section, contaminant bacteria, surgery wound swab.   Abstrak Tujuan: Mengetahui gambaran jenis bakteri pada luka operasi pasien pascaseksio sesarea yang didapat melalui swab luka operasi pasien di Bangsal Kandungan dan Kebidanan RSUD Ulin Banjarmasin dalam periode Agustus-Oktober 2019.Metode: Penelitian ini merupakan studi dekskriptif dengan pendekatan potong lintang. Sebanyak 36 sampel diambil menggunakan metode purposive sampling, tetapi hanya didapatkan 32 isolat bakteri. Sampel swab luka operasi bersih terkontaminasi pasien paska seksio sesarea ditanam pada media pertumbuhan dan diidentifikasi secara mikroskopis untuk selanjutnya diklasifikasikan berdasarkan respon terhadap uji biokimia.Hasil : Analisis secara deksriptif menunjukkan bahwa terdapat tiga jenis bakteri, yaitu Staphylococcus aureus sebanyak 59,3%, Staphylococcus epidermidis sebanyak 25,0%, dan Escherichia coli sebanyak 15,6%.Kesimpulan : Didapatkan 3 jenis bakteri pada hasil swab luka operasi pasien pascaseksio sesarea yang dirawat inap di Bangsal Kandungan dan Kebidanan RSUD Ulin Banjarmasin, yaitu Staphylococcus aureus, Staphylococcus epidermidis, dan Escherichia coli.Kata kunci: seksio sesarea, bakteri kontaminan, swab luka operasi.

scholarly journals Atividade antibacteriana de extratos de folhas de Montrichardia linifera (Arruda) Schott (Araceae)

2015 ◽  
Vol 17 (4 suppl 3) ◽  
pp. 1142-1149 ◽  
Author(s):  
J.A.L MIRANDA ◽  
J.A. ROCHA ◽  
K.M. ARAÚJO ◽  
P.V. QUELEMES ◽  
S.J. MAYO ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Klebsiella Pneumoniae ◽  
Enterococcus Faecalis ◽  
Staphylococcus Epidermidis

RESUMO O uso de plantas medicinais no tratamento de doenças é uma estratégia antiga utilizada por praticamente todas as populações do mundo, e, embora novos antibióticos tenham sido desenvolvidos para o controle de micro-organismos infecciosos, às vezes são ineficazes. Diversos extratos de plantas medicinais têm efeitos antimicrobianos, principalmente quando associados à antibióticos de uso clínico, representando alternativa terapêutica para doenças infecciosas. Montrichardia linifera, conhecida popularmente como aninga, é espécie macrófita, aquática emergente de hábito herbáceo, pertencente a família Araceae e ocorre em áreas alagáveis. A utilidade farmacológica desta espécie é diversificada tendo sido relatada como cicatrizante, antirreumático, antidiurético e expectorante. Devido à relevância no campo etnofarmacológico, ampla utilização na medicina popular e escassez de trabalhos relacionados à atividade antibacteriana desta espécie, objetivou-se com este trabalho avaliar a atividade antibacteriana de extratos alcoólicos de folhas de Montrichardia linifera, coletadas na margem do rio Igaraçu, Parnaíba-PI. O extrato foi testado em oito cepas de bactérias: Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis, Escherichia coli, Klebsiella pneumoniae e Pseudomonas aeruginosa. Foram utilizadas as técnicas de verificação da formação de halos de inibição e determinação das concentrações inibitórias e bactericidas mínimas. Os testes antibacterianos evidenciaram como principais resultados que o extrato metanólico seco (EMS), extrato metanólico fresco (EMF), e o extrato etanólico seco (EES), apresentaram ação antibacteriana, enquanto o extrato etanólico fresco (EEF) não apresentou atividade para as bactérias testadas. O EMS foi o mais eficiente, inibindo o crescimento bacteriano na concentração de 200 μg/mL para E. faecalis, 400 μg/mL para S. aureus, 400 μg/mL para S. epidermidis e 2.000 μg/mL para P. aeruginosa. O EMF obteve CIM de 2.000 μg/mL para E. faecalis e EES obteve CIM de 250 μg/mL para E. faecalis. Os resultados demonstraram que M. linifera constitui fonte eficiente de compostos bioativos antibacterianos. Os estudos sobre as propriedades farmacológicas de plantas da família Araceae são escassos, e os resultados deste trabalho são pioneiros em relação a atividade antibacteriana desta espécie.

scholarly journals Contaminación con bacterias patógenas de estetoscopios del personal médico en un hospital de nivel III en Lima, Perú

2016 ◽  
Vol 27 (2) ◽  
pp. 83 ◽  
Author(s):  
José Enrique Oliva-Menacho ◽  
Marco Antonio García-Hjarles ◽  
José Arturo Oliva-Candela ◽  
Hugo Saturnino De la Cruz-Roca
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Klebsiella Pneumoniae ◽  
Enterobacter Aerogenes ◽  
Macconkey Agar ◽  
Acinetobacter Spp ◽  
Staphylococcus Spp ◽  
Hospital General

Objetivos: Determinar el grado de contaminación bacteriana con bacterias patógenas de los estetoscopios del personal médico en un hospital general de Lima, Perú. Material y métodos: Estudio de tipo observacional, descriptivo y transversal, realizado en el Hospital Nacional Arzobispo Loayza, entre los meses de enero y juniodel 2013. Se estudiaron 124 muestras de estetoscopios del personal médico en las siguientes áreas: UCI 20; neonatología 13; quemados 3; medicina 52; emergencia 36. Se recolectaron las muestras con hisopos humedecidos, en condiciones estériles (En presencia de un mechero de vidrio para alcohol) y luego fueron introducidos en tuboscon preparado de caldo BHI (Infusión cerebro corazón) para ser incubados por 24 horas a 37°C; se cultivó en Agar sangre, Agar MacConkey, Agar manitol y Agar cetrimidepara su posterior determinación de bacterias patógenas por procedimientos bioquímicos ,luego se identificó la susceptibilidad bacteriana con la técnica de Kirby- Bauer. Resultados: De los 124 estetoscopios estudiados; 114 (91,9%) estuvieron contaminados; se aislaron 123 cepasbacterianas: Staphylococcus spp coagulasa negativa 106(86,1%), Staphylococcus aureus 5(4,0%), Enterobacter aerogenes 4 (3,2%), Acinetobacter spp 2(1,6%), Pseudomonas aeruginosa 4(3,2%), Klebsiella Pneumoniae 1(0,8%) y Escherichia coli 1(0,8%). Conclusiones: El aislamiento de bacterias patógenas sugiere que el estetoscopio debe ser considerado como un vector de la infección nosocomial.

scholarly journals Perfil microbiológico y resistencia antibiótica en el servicio de pediatría de una clínica privada, 2016

2019 ◽  
Vol 12 (2) ◽  
pp. 159-165
Author(s):  
Francheska Coricaza-Cuaresma ◽  
Moisés Apolaya-Segura ◽  
Cristian Díaz-Vélez
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Staphylococcus Epidermidis ◽  
E Coli

Objetivo: Describir el perfil microbiológico y de resistencia antibiótica en el servicio de pediatría de la Clínica Jesús del Norte, 2016. Material y métodos: Se diseñó un estudio descriptivo de serie de casos. El estudio se desarrolló en el Servicio de Pediatría de la Clínica Jesús del Norte. Resultados: Se analizaron 1264 muestras de pacienres <15 años con cultivos positivos de orina, heces, sangre y secreción faríngea. El 67,7% fue en <5 años y predomino en mujeres con 59,22%. El germen aislado en mayor frecuencia fue Escherichia coli con 46,68%. Por tipo de muestra el más frecuente fueron: En Coprocultivo: Escherichia coli enteropatógena, Urocultivo: Escherichia coli, Hemocultivo: Staphylococcus epidermidis y en secreción faríngea: Staphylococcus aureus. Llamó la atención la presencia de 105 casos (7,27%) de E. coli BLEE en urocultivos. Conclusiones: Los cultivos positivos fueron más frecuentes en mujeres, en menores de 5 años, atendidos en emergencia y de muestra de heces. Apreciándose alta frecuencia de resistencia a fármacos de primera y segunda línea en diversos agentes etiológicos aislados en las muestras.

scholarly journals Νέες τεχνικές ανάλυσης ειδικών αλληλουχιών DNA και RNA

2006 ◽  
Author(s):  
Δέσποινα Καλογιάννη
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Streptococcus Pneumoniae ◽  
Staphylococcus Epidermidis ◽  
Rt Pcr ◽  
Haemophilus Influenza

Οι μέθοδοι ανίχνευσης και προσδιορισμού ειδικών αλληλουχιών νουκλεϊκών οξέων βασίζονται στην υβριδοποίηση (μοριακή αναγνώριση) του αναλύτη με συμπληρωματικό μόριο DNA/RNA, το οποίο είναι συνδεδεμένο, άμεσα ή έμμεσα, με κατάλληλο ιχνηθέτη για παραγωγή σήματος. Το φάσμα εφαρμογών των μεθόδων υβριδοποίησης συνεχώς διευρύνεται, κυρίως ως αποτέλεσμα της προόδου των προγραμμάτων προσδιορισμού της αλληλουχίας του γονιδιώματος του ανθρώπου και άλλων οργανισμών. Σκοπός της παρούσας διατριβής είναι αφενός η ανάπτυξη μεθόδων υβριδοποίησης που παρέχουν τη δυνατότητα ταυτόχρονου προσδιορισμού πολλών αλληλουχιών νουκλεϊκών οξέων και αφετέρου η απλοποίηση των μεθόδων υβριδοποίησης με τη χρήση βιοαισθητήρα DNA. Στο Κεφάλαιο 1 της διατριβής περιγράφεται η αρχή της αλυσιδωτής αντίδρασης πολυμεράσης (PCR) και η αντίστροφη μεταγραφή-PCR (RT-PCR) ως οι πιο σημαντικές τεχνικές εκθετικού πολλαπλασιασμού ειδικών αλληλουχιών DNA και RNA, οι οποίες αποτελούν αναπόσπαστο μέρος κάθε σύγχρονης μεθόδου ανάλυσης νουκλεϊκών οξέων. Παρουσιάζεται επίσης η τεχνική της ποσοτικής PCR και διάφορες μέθοδοι προσδιορισμού των προϊόντων της PCR. Στο Κεφάλαιο 2 γίνεται παρουσίαση των δοκιμασιών υβριδοποίησης νουκλεϊκών οξέων, οι οποίες αποτελούν και τον ακρογωνιαίο λίθο της αναλυτικής χημείας του DNA και RNA. Περιγράφεται η εξέλιξη των ιχνηθετών από τα ραδιοϊσότοπα μέχρι τους πιο σύγχρονους μη ραδιενεργούς ιχνηθέτες που παρέχουν υψηλή ανιχνευσιμότητα και δυνατότητες αυτοματοποίησης. Παρουσιάζονται οι ομογενείς και ετερογενείς δοκιμασίες υβριδοποίησης και διάφορες παραλλαγές που διευκολύνουν την εκτέλεση τους. Στο Κεφάλαιο 3 παρέχεται σύντομη περιγραφή της αρχής λειτουργίας και της οργανολογίας της κυτταρομετρίας ροής, η οποία αποτελεί ένα ισχυρό αναλυτικό εργαλείο για τη διερεύνηση ιδιοτήτων μικροσωματιδίων (π.χ. κυττάρων αλλά και συνθετικών μικροσφαιριδίων). Αντικείμενο του Κεφαλαίου 4 είναι η ανάπτυξη πολλαπλών φθορισμομετρικών δοκιμασιών υβριδοποίησης DNA και RNA σε εναιώρημα φασματικά διακριτών μικροσφαιριδίων πολυστυρενίου. Κάθε μικροσφαιρίδιο περιέχει δύο φθορίζουσες χρωστικές σε συγκεκριμένη αναλογία και με αυτό τον τρόπο έχει καταστεί ένα φασματικά κωδικοποιημένο στερεό υπόστρωμα για την εκτέλεση δοκιμασιών υβριδοποίησης. Ειδικά ολιγονουκλεοτίδια-ανιχνευτές προσδένονται ομοιοπολικά στην επιφάνεια κάθε μικροσφαιριδίου. Η αλληλουχία του DNA- ή RNA-στόχου (αναλύτης) υβριδοποιείται με τα ακινητοποιημένα ολιγονουκλεοτίδια και τα υβρίδια ποσοτικοποιούνται με άλλη φθορίζουσα ουσία. Το εναιώρημα αναλύεται με κυτταρομετρητή ροής με ανιχνευτή φθορισμού επαγώμενου με δύο πηγές λέιζερ. Μελετήθηκε ο προσδιορισμός μονόκλωνου DNA, δίκλωνου DNA και RNA και πραγματοποιήθηκαν πειράματα ανάλυσης γονιδιακής έκφρασης σε ασθενείς με λευχαιμία. Στο Κεφάλαιο 5 παρουσιάζεται ο πρώτος βιοαισθητήρας DNA τύπου εμβαπτιζόμενης ταινίας ξηρών αντιδραστηρίων για την ανίχνευση 7 χρωμοσωμικών αλληλομεταθέσεων, οι οποίες σχετίζονται άμεσα με την οξεία ή τη χρόνια λευχαιμία. Ο βιοαισθητήρας βασίζεται σε ολιγονουκλεοτίδια συζευγμένα με νανοσωματίδια χρυσού και επιτρέπει την ταυτοποίηση των αλληλομεταθέσεων δια γυμνού οφθαλμού, έπειτα από πολλαπλασιασμό με RT-PCR. Σε αντίθεση με τις μέχρι τώρα χρησιμοποιούμενες μεθόδους ανίχνευσης αλληλομεταθέσεων, η ανάλυση με το βιοαισθητήρα είναι απλή, δεν απαιτεί ειδικά όργανα και αποφεύγει πολλά στάδια επωάσεων και εκπλύσεων. Αντικείμενο του Κεφαλαίου 6 της διατριβής είναι η ανάπτυξη μεθόδου υβριδοποίησης DNA για την ταχεία ανίχνευση βακτηριακής επιμόλυνσης σε αρθροπλαστικές επεμβάσεις, ένα σημαντικό πρόβλημα στην ορθοπεδική. Απομονώνεται DNA από δείγματα αρθρικού υγρού, ακολουθεί πολλαπλασιασμός με PCR χρησιμοποιώντας καθολικούς εκκινητές και ανίχνευση των προϊόντων με βιοαισθητήρα DNA. Ανιχνεύονται οι μικροοργανισμοί Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis, Haemophilus influenza, Enterococcus faesium και Streptococcus pneumoniae. Στο Κεφάλαιο 7 περιγράφεται η εφαρμογή του βιοαισθητήρα DNA για την ανίχνευση γενετικά τροποποιημένων οργανισμών (GMO) σε τρόφιμα. Αν και η παρουσία GMO μπορεί να διαπιστωθεί με ανίχνευση είτε της νέας πρωτεΐνης που συνθέτει το διαγονιδιακό φυτό ή της αλληλουχίας DNA που έχει εισαχθεί σ’ αυτό, το DNA είναι ο προτιμότερος αναλύτης λόγω της μεγαλύτερης σταθερότητάς του, ιδιαίτερα σε επεξεργασμένα τρόφιμα. Χαρακτηριστικές αλληλουχίες DNA που περιέχονται στα περισσότερα GMO, δηλαδή ο προαγωγέας 35S και ο τερματιστής NOS, πολλαπλασιάζονται με PCR και τα προϊόντα ανιχνεύονται με δοκιμασία υβριδοποίησης στο βιοαισθητήρα DNA. Η παρούσα εργασία εισάγει, για πρώτη φορά στο πεδίο της ανάλυσης GMO, έναν βιοαισθητήρα DNA τύπου εμβαπτιζόμενης ταινίας ξηρών αντιδραστηρίων.

scholarly journals Combination Effects of Cymbopogon sp. Essential Oil on Selected Bacteria

2018 ◽  
Vol 1 (1) ◽  
Author(s):  
Nur Aishah Abdul Wahab ◽  
Hairul Shahril Muhamad ◽  
Nabilah Ahmad Alhadi ◽  
Salina Mat Radzi ◽  
Maryam Mohamed Rehan ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Bacillus Subtilis ◽  
Essential Oils ◽  
Staphylococcus Epidermidis ◽  
Antimicrobial Agents ◽  
Synergistic Effects ◽  
Combination Effects ◽  
Cymbopogon Flexuosus ◽  
Combination Study

Combination effects between Cymbopogon flexuosus and Cymbopogon nardus essential oils were studied to determine whether the combination could emerge as better and more powerful antimicrobial agents against six selected bacteria includes Bacillus subtilis, Escherichia coli, Salmonella typhimurium, Serratia marcescens, Staphylococcus aureus, and Staphylococcus epidermidis. This combination study exhibited 40.67% additive, 28.67% antagonistic, 16.00% indifferent and 14.66% synergistic effects. C. flexuosus and C. nardus essential oils in combination showed a high inhibitory activity against S. aureus with 16% synergistic, 64% additive and 20% indifferent effects.

scholarly journals Bacteria Sensitivity Pattern of Caesarean Section Wound on Selected Antibiotics in Ulin General Hospital Banjarmasin

2020 ◽  
Vol 16 (2) ◽  
pp. 73
Author(s):  
Nurhafizah Rafiani ◽  
Renny Aditya ◽  
Noor Muthmainah
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Caesarean Section ◽  
Staphylococcus Epidermidis ◽  
Antibiotic Sensitivity ◽  
Cross Sectional ◽  
Surgical Wounds ◽  
Sensitivity Pattern ◽  
Antibiotic Sensitivity Test ◽  
Bacterial Sensitivity

Abstract: A cesarean delivery increases the risk of wound infection it should be prevented by using antibiotics. This study was to determine the pattern of bacterial sensitivity in surgical wounds of cesarean section patients for selected antibiotics, i.e ceftriaxone, cefixime, sulbactam ampicillin, ciprofloxacin, clindamycin and gentamicin.This observational study was conducted at the Ulin Public Hospital in Banjarmasin from July to September 2019. Using a cross sectional approach Samples of bacteria were identidied from 36 that were pusposively sampled, i.e., Staphylococcus aureus, Staphylococcus epidermidis and Escherichia coli. Antibiotic sensitivity test showed that Staphylococcus aureus was sensitive against gentamicin (100%),whereas Staphylococcus epidermidis and Escherichia coli. Were intermediately sensitive towards gentamicin (62.5%) and ceftriaxone (80%) Keywords: Antibiotic susceptibility, caesarean section, surgical wound

scholarly journals UTILIZAÇÃO DE GÁS OZÔNIO NA DESINFECÇÃO DE RESÍDUOS DE SERVIÇOS DE SAÚDE

2018 ◽  
Vol 7 (2) ◽  
pp. 125-139
Author(s):  
Thais Nogueira Gonzaga ◽  
Dora Inés Kozusny-Andreani
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Candida Albicans ◽  
Health Care Services ◽  
Pathogenic Bacteria ◽  
Ozone Treatment ◽  
Microbiological Analysis ◽  
Before And After ◽  
Staphylococcus Spp

Nesta pesquisa objetivou-se avaliar a viabilidade técnica da aplicação de ozônio como bactericida e fungicida em amostras de resíduos de serviços de saúde potencialmente infectantes. Foram determinados os     micro-organismos presentes nos resíduos gerados em um hospital particular. Para realização das análises microbiológicas e o tratamento com ozônio o material foi particulado e homogeneizado. As análises microbiológicas foram realizadas antes e após a ozonização.Para os testes de desinfecção foram retirados 10,0g de amostra que foi submetida à ozonização por 5, 10, 15, 20 e 25 minutos com doses de 140,0; 280,0; 420,0; 560,0 e 700,0mg L-1 de ozônio, respectivamente. Verificou-se presença de mesófilos totais, coliformes totais e termotolerantes, Escherichia coli, Pseudomonas aeruginosa, Proteus spp., Staphylococcus aureus, Staphylococcus spp, Candida albicans e Rhizopus spp. O ozônio foi eficiente para eliminação de todos os micro-organismos em 20 minutos; nos primeiros cinco minutos de exposição ao gás verificou-se redução superior a 98%.Palavras-chave: Bactérias patogênicas. Fungos. Ozonização. USING OZONE GAS FOR DISINFECTION OF SOLID WASTE FROM HEALTH CARE SERVICES ABSTRACT: The aim of this research was to evaluate the technical viability of the application of ozone as bactericide and fungicide in samples of potentially infectious health services residues. The microorganisms present in the waste generated in a private hospital were determined. The material was particulated and homogenized to perform the microbiological analysis and to undergo ozone treatment. Microbiological analysis was performed before and after ozonization. For the disinfection tests, 10.0g of sample were removed and submitted to ozonization for 5, 10, 15, 20 and 25 minutes with 140,0; 280,0; 420,0; 560,0 and 700,0mg doses of L-1 of ozone, respectively. It was verified the presence of total mesophiles, total and thermotolerant coliforms, Escherichia coli, Pseudomonas aeruginosa, Proteus spp., Staphylococcus aureus, Staphylococcus spp, Candida albicans and Rhizopus spp. Ozone was efficient while eliminating all microorganisms in 20 minutes; in the first five minutes of gas exposure, the reduction was greater than 98%.Keywords: Pathogenic bacteria. Fungi. Ozonization.

Persistent contamination of Salmonella, Campylobacter, Escherichia coli, and Staphylococcus aureus at a broiler farm in New Zealand

2020 ◽  
Vol 66 (3) ◽  
pp. 171-185 ◽  
Author(s):  
Kristine Castañeda-Gulla ◽  
Evelyn Sattlegger ◽  
Anthony N. Mutukumira
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Quantitative Polymerase Chain Reaction ◽  
Control Measures ◽  
Poultry Production ◽  
Chain Reaction ◽  
Pathogen Control ◽  
Broiler Farm ◽  
Polymerase Chain ◽  
And Staphylococcus Aureus

Intensive poultry production due to public demand raises the risk of contamination, creating potential foodborne hazards to consumers. The prevalence and microbial load of the pathogens Campylobacter, Salmonella, Staphylococcus aureus, and Escherichia coli was determined by standard methods at the farm level. After disinfection, swab samples collected from wall crevices, drinkers, and vents were heavily contaminated, as accumulated organic matter and dust likely protected the pathogens from the disinfectants used. The annex floor also showed high microbial concentrations, suggesting the introduction of pathogens from external environments, highlighting the importance of erecting hygiene barriers at the entrance of the main shed. Therefore, pathogen control measures and proper application of disinfectants are recommended as intervention strategies. Additionally, quantitative polymerase chain reaction (qPCR) was evaluated as a quantification tool. qPCR showed limitations with samples containing low microbial counts because of the low detection limit of the method. Thus, bacterial pre-enrichment of test samples may be necessary to improve the detection of pathogens by qPCR.

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