Measurement of viscoelastic properties of treated and untreated cancer cells using passive microrheology

2021 ◽  
Author(s):  
Devesh Bekah
Keyword(s):  
Cancer Cells ◽  
Viscoelastic Properties ◽  
Elastic Moduli ◽  
Fold Increase ◽  
Glycerol Water ◽  
Particle Tracking Microrheology ◽  
Ultrasound Backscatter ◽  
One Step ◽  
Poly Ethylene ◽  
Mcf 7

Experiments have shown that there is an increase in ultrasound backscatter from cells during cell death. Since cell scattering depends on the mechanical property variations, one step towards a better understanding of the phenomenon involves measuring the cells' viscoelastic properties. Two promising techniques used for such studies are particle tracking microrheology (1P) and two-point microrheology (2P). The main aim of this work is to develop and test the ability of both to measure changes in viscous and elastic moduli of breast cancer cells during chemotherapeutic treatments. First, the viscosities of glycerol-water mixtures measured using microrheology were found to be within 5% of rheometer values. The viscous and elastic moduli of 4% and 6% poly(ethylene oxide) solutions were successfully measured at 30°C and 37°C. For MCF-7 cells, a 10-fold increase in the elastic modulus was observed using 1P, without a corresponding increase in the viscous modulus. Thus, it was shown that MCF-7 cells undergo an increase in stiffness during apoptosis.

scholarly journals Measurement of viscoelastic properties of treated and untreated cancer cells using passive microrheology

2021 ◽  
Author(s):  
Devesh Bekah
Keyword(s):  
Cancer Cells ◽  
Viscoelastic Properties ◽  
Elastic Moduli ◽  
Fold Increase ◽  
Glycerol Water ◽  
Particle Tracking Microrheology ◽  
Ultrasound Backscatter ◽  
One Step ◽  
Poly Ethylene ◽  
Mcf 7

Experiments have shown that there is an increase in ultrasound backscatter from cells during cell death. Since cell scattering depends on the mechanical property variations, one step towards a better understanding of the phenomenon involves measuring the cells' viscoelastic properties. Two promising techniques used for such studies are particle tracking microrheology (1P) and two-point microrheology (2P). The main aim of this work is to develop and test the ability of both to measure changes in viscous and elastic moduli of breast cancer cells during chemotherapeutic treatments. First, the viscosities of glycerol-water mixtures measured using microrheology were found to be within 5% of rheometer values. The viscous and elastic moduli of 4% and 6% poly(ethylene oxide) solutions were successfully measured at 30°C and 37°C. For MCF-7 cells, a 10-fold increase in the elastic modulus was observed using 1P, without a corresponding increase in the viscous modulus. Thus, it was shown that MCF-7 cells undergo an increase in stiffness during apoptosis.

Effet radiosensibilisateur de l'acide linoléique conjugué chez les cellules cancéreuses du sein MCF-7 et MDA-MB-231

2004 ◽  
Vol 82 (2) ◽  
pp. 94-102 ◽  
Author(s):  
Geneviève Drouin ◽  
Annie Douillette ◽  
Pierre Lacasse ◽  
Benoit Paquette
Keyword(s):  
Breast Cancer ◽  
Linoleic Acid ◽  
Cancer Cells ◽  
Conjugated Linoleic Acid ◽  
Breast Cancer Cells ◽  
Fold Increase ◽  
Breast Cancer Cell Lines ◽  
Apoptotic Pathways ◽  
Induced Apoptosis ◽  
Mcf 7

Apoptotic pathways in breast cancer cells are frequently altered, reducing the efficiency of radiotherapy. Conjugated linoleic acid (CLA), known to trigger apoptosis, was tested as radiosensitizer in breast cancer cells MCF-7 and MDA-MB-231. The CLA-mix, made up of the isomers CLA-9cis 11trans and CLA-10trans 12cis, was compared to three purified isomers, i.e., the CLA-9cis 11cis, CLA-9cis 11trans, and CLA-10trans 12cis. Using the apoptotic marker YO-PRO®-1, the CLA-9cis 11cis at 50 µmol/L turned out to be the best apoptotic inducer leading to a 10-fold increase in MCF-7 cells and a 2,5-fold increase in MDA-MB-231 cells, comparatively to the CLA-mix. Contrary to previous studies on colorectal and prostate cancer cells, CLA-10trans 12cis does not lead to an apoptotic response on breast cancer cell lines MCF-7 and MDA-MB-231. Our results also suggest that the main components of the CLA-mix (CLA-9cis 11trans and CLA-10trans 12cis) are not involved in the induction of apoptosis in the breast cancer cells studied. A dose of 5 Gy did not induce apoptosis in MCF-7 and MDA-MB-231 cells. The addition of CLA-9cis 11cis or CLA-mix has allowed us to observe a radiation-induced apoptosis, with the CLA-9cis 11cis being about 8-fold better than the CLA-mix. CLA-9cis 11cis turned out to be the best radiosensitizer, although the isomers CLA-9cis 11trans and CLA-10trans 12cis have also reduced the cell survival following irradiation, but using a mechanism not related to apoptosis. In conclusion, the radiosensitizing property of CLA-9cis 11cis supports its potential as an agent to improve radiotherapy against breast carcinoma.Key words: breast cancer, conjugated linoleic acid (CLA), radiotherapy, apoptosis.

scholarly journals Regulation of cell cycle and cyclins by 16alpha-hydroxyestrone in MCF-7 breast cancer cells

2001 ◽  
Vol 27 (3) ◽  
pp. 293-307 ◽  
Author(s):  
JS Lewis ◽  
TJ Thomas ◽  
CM Klinge ◽  
MA Gallo ◽  
T Thomas
Keyword(s):  
Breast Cancer ◽  
Cell Cycle ◽  
Dna Synthesis ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Cell Cycle Progression ◽  
Fold Increase ◽  
Cycle Progression ◽  
Synchronized Cells ◽  
Mcf 7

It has been suggested that alterations in estradiol (E(2)) metabolism, resulting in increased production of 16alpha-hydroxyestrone (16alpha-OHE(1)), is associated with an increased risk of breast cancer. In the present study, we examined the effects of 16alpha-OHE(1)on DNA synthesis, cell cycle progression, and the expression of cell cycle regulatory genes in MCF-7 breast cancer cells. G(1) synchronized cells were treated with 1 to 25 nM 16alpha-OHE(1) for 24 and 48 h. [(3)H]Thymidine incorporation assay showed that 16alpha-OHE(1) caused an 8-fold increase in DNA synthesis compared with that of control cells, whereas E(2) caused a 4-fold increase. Flow cytometric analysis of cell cycle progression also demonstrated the potency of 16alpha-OHE(1) in stimulating cell growth. When G(1) synchronized cells were treated with 10 nM 16alpha-OHE(1) for 24 h, 62+/-3% of cells were in S phase compared with 14+/-3% and 52+/-2% of cells in the control and E(2)-treated groups respectively. In order to explore the role of 16alpha-OHE(1) in cell cycle regulation, we examined its effects on cyclins (D1, E, A, B1), cyclin dependent kinases (Cdk4, Cdk2), and retinoblastoma protein (pRB) using Western and Northern blot analysis. Treatment of cells with 10 nM 16alpha-OHE(1) resulted in 4- and 3-fold increases in cyclin D1 and cyclin A, respectively, at the protein level. There was also a significant increase in pRB phosphorylation and Cdk2 activation. In addition, transient transfection assay using an estrogen response element-driven luciferase reporter vector showed a 15-fold increase in estrogen receptor-mediated transactivation compared with control. These results show that 16alpha-OHE(1) is a potent estrogen capable of accelerating cell cycle kinetics and stimulating the expression of cell cycle regulatory proteins.

scholarly journals Essential oil loaded pectin/chitosan nanoparticles preparation and optimization via Box–Behnken design against MCF-7 breast cancer cell lines

RSC Advances ◽  
2020 ◽  
Vol 10 (15) ◽  
pp. 8703-8708 ◽  
Author(s):  
Olivia A. Attallah ◽  
Amro Shetta ◽  
Fatma Elshishiny ◽  
Wael Mamdouh
Keyword(s):  
Breast Cancer ◽  
Essential Oil ◽  
Cell Viability ◽  
Cancer Cells ◽  
Chitosan Nanoparticles ◽  
Fold Increase ◽  
Breast Cancer Cell Lines ◽  
Normal Cells ◽  
Box Behnken Design ◽  
Mcf 7

Pec/CS/JO formulated nanocomposite showed a 13-fold increase in potency against cancer cells, whereas cell viability of normal cells wasn't affected.

PEGylation of Conjugated Linoleic Acid and its Application as an Anti-Cancer Prodrug

2007 ◽  
Vol 342-343 ◽  
pp. 441-444 ◽  
Author(s):  
Ji Hye Seo ◽  
Hyun Seuk Moon ◽  
Ding Ding Guo ◽  
Hong Gu Lee ◽  
Yun Jaie Choi ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Linoleic Acid ◽  
Cancer Cells ◽  
Conjugated Linoleic Acid ◽  
Breast Cancer Cells ◽  
Cancer Cell Proliferation ◽  
Anti Cancer ◽  
Ester Linkage ◽  
Poly Ethylene ◽  
Mcf 7

The objective of this study is to investigate whether the PEGylated conjugated linoleic acid (PCLA) as an anti-cancer prodrug can have favorable stability, biological activity, and prevention of proliferation in MCF-7 breast cancer cells for anti-cancer when compared with conjugated linoleic acid (CLA) itself. The CLA was simply coupled to poly(ethylene glycol) (PEG) at melting state without solvent or catalyst through ester linkage between carboxylic group of CLA and hydroxyl one of PEG. The results showed that the half life of PCLA was 55h in cell culture medium at pH 7.4 and 37°C. Apoptosis of MCF-7 breast cancer cells were induced by not only CLA- but PCLA-treatment with increasing concentrations whereas PCLA increased cell viability when compared with CLA itself. These results indicate that the PCLA is a more stable and valuable prodrug in that it has good stability and inhibition of cancer cell proliferation.

scholarly journals 2-Methoxy-estradiol Loaded Alpha Lipoic Acid Nanoparticles Augment Cytotoxicity in MCF-7 Breast Cancer Cells

Dose-Response ◽  
2021 ◽  
Vol 19 (4) ◽  
pp. 155932582110550
Author(s):  
Nabil A. Alhakamy ◽  
Mohammed W. Al-Rabia ◽  
Hani Z. Asfour ◽  
Samah Alshehri ◽  
Waleed S. Alharbi ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Anticancer Drugs ◽  
Lipoic Acid ◽  
Breast Cancer Cells ◽  
Entrapment Efficiency ◽  
Fold Increase ◽  
Alpha Lipoic Acid ◽  
P53 Expression ◽  
Mcf 7

The therapeutic effectiveness of anticancer drugs with a selective target for the nucleus of cancer cells may be improved by experimental approaches. In this regard, the formulation of anticancer drugs is considered one of the best ways to improve their effectiveness in targeting cancerous tissues. To enhance the anticancer activity of 2-methoxy-estradiol (2 ME) for breast cancer, 2-methoxyestradiol loaded alpha lipoic acid nanoparticles have been formulated. The prepared formula was observed to be spherical with a nanometer-scale and low PDI size (.234). The entrapment efficiency of the 2ME-ALA NPs was 87.32 ± 2.21% with > 85% release of 2 ME within 24 h. There was a 1.2-fold increase in apoptosis and a 3.46-fold increase in necrosis of the MCF-7 cells when incubated with 2ME-ALA NPs when compared to control cells. This increased apoptosis was also associated with increased ROS and increased p53 expression in 2ME-ALA NPs treated cells compared to the raw-2 ME group. Evaluation of cell-cycle data showed a substantial arrest of the G2-M phase of the MCF-7 cells when incubated with 2ME-ALA NPs. At the same time, a dramatically increased number of pre-G1 cells showed the increased apoptotic potential of the 2 ME when administered via the proposed formulation. In the end, the differential upregulation of caspase-3, p53, and ROS in MCF-7 cells established the superiority of the 2ME-ALA-Ms approach in targeting breast cancer. In summary, these results demonstrate that 2ME-ALA NPs are an efficient delivery tool for controlling the growth of breast cancer cells.

scholarly journals Multi-Modulation of Doxorubicin Resistance in Breast Cancer Cells by Poly(l-histidine)-Based Multifunctional Micelles

Pharmaceutics ◽  
2019 ◽  
Vol 11 (8) ◽  
pp. 385 ◽  
Author(s):  
Li Jia ◽  
Nan Jia ◽  
Yan Gao ◽  
Haiyang Hu ◽  
Xiuli Zhao ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Multi Drug Resistance ◽  
Glycoprotein P ◽  
Passive Targeting ◽  
Poly Ethylene ◽  
Targeting Ability ◽  
Mcf 7

Even though the reversal of multi-drug resistance (MDR) by numerous nanoparticles has been extensively studied, limited success has been achieved. To overcome this barrier, we report a rationally-designed pH-sensitive micelle, in which doxorubicin (Dox) and resveratrol (Res) were co-loaded. The micelle was based on methoxy poly (ethylene glycol)-poly(d,l-lactide)-poly(l-histidine) (mPEG-PLA-PHis), which integrated passive targeting, endo-lysosomal escape and pH-responsive payloads release. At a physiological pH of 7.4 (slightly alkali), Dox and Res were incorporated into the micelles core using the thin-film hydration method (pH-endoSM/Dox/Res). After cellular uptake, the micelles exhibited an enhanced dissociation in response to the acidic endosomes, triggering the release of Res and Dox. Furthermore, Res was observed to synergistically improve the cytotoxicity of Dox by down-regulating the P-glycoprotein (P-gp) expression, decreasing the membrane potential of the mitochondrial and ATP level, as well as inducing cell apoptosis mediated by mitochondria. The pH-endoSM/Dox/Res showed a prominent ability to decrease the IC50 of Dox by a factor of 17.38 in cell cytotoxicity against the MCF-7/ADR cell line. In vivo distribution demonstrated the excellent tumor-targeting ability of the pH-endoSM/Dox/Res. All results indicated that pH-endoSM/Dox/Res held great potential for the treatment of Dox-resistance breast cancer cells.

scholarly journals Utilization of Lithium Incorporated Mesoporous Silica for Preventing Necrosis and Increase Apoptosis in Different Cancer Cells.

2018 ◽  
Author(s):  
Kamel A. Saleh ◽  
Sharah A. Aldulmani ◽  
Nasser Awwad ◽  
Mohammad Y. Alfaifi ◽  
Mohamed S. Hamdy ◽  
...  
Keyword(s):  
Mesoporous Silica ◽  
Cancer Cells ◽  
Active Sites ◽  
Drug Carrier ◽  
Sol Gel ◽  
Ic50 Value ◽  
One Step ◽  
Synthesis Procedure ◽  
New Compounds ◽  
Mcf 7

There are many molecules used as drug carrier. TUD-1 is a newly synthesized mesoporous silica (SM) molecule possess two important features; consists of mesoporous so it is very suitable to be drug carrier in addition to that it has the ability to induce apoptosis in cancer cells. However, the effect of TUD-1 appears to act as cell death inducer, regardless of whether it is necrosis or apoptosis. Unfortunately, recent studies indicate that a proportion of cells undergo necrosis rather than apoptosis, which limits the use of TUD-1 as a secure treatment. On the other hand, lithium considered as necrosis inhibitor element. Hence, current study based on the idea of production a new Li/TUD-1 by incorporated mesoporous silica (TUD-1 type) with lithium in order to produce a new compound that has the ability to activate apoptosis by mesoporous silica (TUD-1 type) and at the same time can inhibit the activity of necrosis by lithium. Herein, lithium was incorporated in TUD-1 mesoporous silica by using sol-gel technique in one step synthesis procedure. Moreover, lithium was incorporated in TUD-1 with different loading in order to form different active sites such as isolated lithium ions, nanoparticles of Li2O, and bulky crystals of Li2O. The ability of the new compounds to induce apoptosis and prevent necrosis was evaluated on three different types of cancer cell lines which are; liver HepG-2, Breast MCF-7 and colon HCT116. The obtained results show that Li/TUD-1has the ability to control necrosis and thus reduce the side effects of treatments containing silica in the case of lithium has been added to them, especially in chronic cases. This has been demonstrated by the significant increase in the IC50 value and cell viability comparing to control groups. Consequently, the idea is new, so it definitely needs more develop and test with materials that have more apoptotic impact than silica in order to induce apoptosis without induction of necrosis.

scholarly journals Cytotoxic Effect of Paclitaxel and Lapatinib Co-Delivered in Polylactide-co-Poly(ethylene glycol) Micelles on HER-2-Negative Breast Cancer Cells

Pharmaceutics ◽  
2019 ◽  
Vol 11 (4) ◽  
pp. 169 ◽  
Author(s):  
Alicja Zajdel ◽  
Adam Wilczok ◽  
Katarzyna Jelonek ◽  
Monika Musiał-Kulik ◽  
Aleksander Foryś ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Binary Mixture ◽  
Ethylene Glycol ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Cytotoxic Effect ◽  
Poly Ethylene Glycol ◽  
Her 2 ◽  
Poly Ethylene ◽  
Mcf 7

To find better strategies to enhance the cytotoxic effect of paclitaxel (PTX) and lapatinib (LAP) against breast cancer cells, we analyzed the efficacy of a novel delivery system containing polylactide-co-poly(ethylene glycol) (PLA-PEG) filomicelles of over 100 nm in length and spherical micelles of approximately 20 nm in diameter. The 1H NMR measurements confirmed the incorporation of PTX and LAP into micelles. Analysis of the drug release mechanism revealed the diffusion-controlled release of LAP and anomalous transport of PTX. Drug content analysis in lyophilized micelles and micellar solution showed their good storage stability for at least 6 weeks. Blank micelles, LAP-loaded micelles and free LAP did not affect MCF-7 breast cancer cell proliferation, suggesting that the cytotoxicity of PTX-, PTX/LAP-loaded micelles, and the binary mixture of free PTX and LAP was solely caused by PTX. PTX/LAP-loaded micelles showed greater toxicity compared to the binary mixture of PTX and LAP after 48 h and 72 h. Only free PTX alone induced P-gp activity. This study showed the feasibility of using a LAP and PTX combination to overcome MDR in MCF-7 cells, particularly when co-loaded into micelles. We suggest that PTX/LAP micelles can be applicable not only for the therapy of HER-2-positive, but also HER-2-negative breast cancers.

Sign in / Sign up

Export Citation Format

Share Document