scholarly journals SKRINING BAKTERI TERMOFILIK POTENSIAL AMILOLITIK DARI SUMBER AIR PANAS WAY BELERANG KALIANDA LAMPUNG SELATAN

2021 ◽  
Vol 6 (1) ◽  
pp. 1-7
Author(s):  
Sefi Desfeni Mawati ◽  
Esti Harpen ◽  
Hilma Putri Fidyandini
Keyword(s):  
Incubation Temperature ◽  
Hot Spring ◽  
Thermophilic Bacteria ◽  
Pseudomonas Stutzeri ◽  
Optimum Temperature ◽  
Bacterial Isolates ◽  
Inhibitory Zone ◽  
Biochemical Tests ◽  
Amylolytic Enzyme ◽  
Thermostable Amylase

Thermophilic bacteria that produced amylase and protease have been isolated from Way Belerang hot spring, Kalianda, South Lampung. This research aims to screen and identify thermophilic bacteria that have the potential to produce thermostable amylase and protease enzymes.The research procedures included sampling, isolation of enzyme-producing thermophilic bacteria, a series of phenotypic and biochemical tests, and molecular identification by 16s rRNA. This study used 2 treatments, namely incubation temperature 37 and 50 ºC with 3 repetitions. The results showed that the optimum temperature for growth of thermophilic bacterial isolates and thermophilic bacterial isolates producing amylase enzymes was 50ºC. The bacteria isolate that had the best amylolytic enzyme activity was Isolate A.WB.50.1 with a diameter of the inhibitory zone was 15.44 mm. Isolate A.WB.50.1 has been identified by the species Pseudomonas stutzeri.

scholarly journals PRODUCTION AND CHARACTERIZATION OF CHITINASE ENZYMES FROM SULILI HOT SPRING IN SOUTH SULAWESI, Bacillus sp. HSA,3-1a

2010 ◽  
Vol 10 (2) ◽  
pp. 256-260 ◽  
Author(s):  
Hasnah Natsir ◽  
Abd. Rauf Patong ◽  
Maggy Thenawidjaja Suhartono ◽  
Ahyar Ahmad
Keyword(s):  
Hot Spring ◽  
Thermophilic Bacteria ◽  
Extracellular Enzyme ◽  
Colloidal Chitin ◽  
Bacillus Sp ◽  
Optimum Temperature ◽  
South Sulawesi ◽  
Sds Page ◽  
Physiological Analysis

Chitinase is an extracellular enzyme which is capable in hydrolyzing insoluble chitin to its oligomeric and monomeric components. The enzyme produced by thermophilic bacteria was screened and isolated from Sulili hot spring in Pinrang, South Sulawesi, Indonesia. The gram positive spore forming rod shape bacteria was identified as Bacillus sp. HSA,3-1a through morphological and physiological analysis. The production of chitinase enzyme was conducted at various concentration of colloidal chitin at a pH of 7.0 and a temperature of 55 °C. The bacteria optimally was produced the enzyme at a colloidal chitin concentration of 0.5% after 72 h of incubation. The optimum temperature, pH and substrate concentration of chitinase were 60 °C, 7.0 and 0.3%, respectively. The enzyme was stable at a pH of 7.0 and a temperature of 60 °C after 2 h of incubation. The chitinase activities was increased by addition of 1 mM Mg2+, Ca2+ and Mn2+ ions, whereas the activities were  decreased by 1 mM Co2+, Fe2+ and Zn2 ions. The molecular weight of the crude enzyme was determined using SDS-PAGE analysis. Five protein fractions were obtained from SDS-PAGE, with MWs of 79, 71, 48, 43 and 22 kDa.   Keywords: colloidal chitin, thermophilic bacteria, chitinase

scholarly journals PRODUKSI PROTEASE ALKALI DAN KERATINASE DARI Brevibacillus agri A-03 TERMOFILIK

2015 ◽  
Vol 4 (1) ◽  
pp. 7
Author(s):  
Anthoni Agustien ◽  
Jetty Nurhajati ◽  
Linar Z. Udin ◽  
Pingkan Aditiawati
Keyword(s):  
Protein Content ◽  
Incubation Temperature ◽  
Specific Activity ◽  
Hot Spring ◽  
Thermophilic Bacteria ◽  
Exponential Phase ◽  
Medium Ph ◽  
Enzym Activity ◽  
Medium Type ◽  
Brevibacillus Agri

 ABSTRAK Protease alkaline and keratinase are a group of protease enzym which have important value in detergen industry and skin tannery. Brevibacillus agri A-03 is thermophilic bacteria isolate that comes from Ambayan Sumatera Barat hot spring and has the ability to produce protease and keratinase. The purpose of this research is to get protease alkaline and thermostable keratinase from Brevibacilus agri-A03. thermostable enzym is produced from enzym production enzym that contains kasein and keratin at various medium pH, inoculum incubation temperature and medium type. Enzym activity is measured by modified Walker methode, protein content is measured by Lowry methode. Protease alkaline is produced at exponential phase, maximum at 18th hours of incubation and keratinase is produced at stationer phase, maximum at 22nd hours. Both enzym is produced optimically at medium pH condition 9.0; incubation temperature 55°C, inoculum 5% by using modified Johnvesly and Naik medium with each protease and keratinase specific activity 1.927 and 1.047 U/mg  Keywords: Protease alkaline, Keratinase, Thermofilic, Brevibacillus agri A-03   

scholarly journals Susceptibility of Klebsiella Pneumoniae Isolated from Pus Specimens of Post-Surgery Patients in Medan, Indonesia to Selected Antibiotics

2019 ◽  
Vol 7 (22) ◽  
pp. 3861-3864 ◽  
Author(s):  
Popi Patilaya ◽  
Dadang Irfan Husori ◽  
Lany Marhafanny
Keyword(s):  
Klebsiella Pneumoniae ◽  
Klebsiella Pneumonia ◽  
Good Sensitivity ◽  
Bacterial Isolates ◽  
Inhibitory Zone ◽  
Biochemical Tests ◽  
Post Surgery ◽  
Low Sensitivity

AIM: This study was to determine the sensitivity of Klebsiella pneumonia isolated from pus specimens of post-surgery patients in Medan, Indonesia to selected antibiotics. METHODS: Samples were collected at the Laboratory of Microbiology, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia. The isolated bacteria were identified by Gram’s stain, colony characteristics, and biochemical tests. Susceptibility of K. pneumoniae isolates were tested to selected antibiotics including amikacin, meropenem, levofloxacin, ciprofloxacin, co-trimoxazole, ceftazidime, cefoperazone, cefuroxime, cefepime, cefotaxime, tetracycline, chloramphenicol, amoxicillin and ampicillin with Kirby Bauer method by measuring the inhibitory zone. RESULTS: A total of 20 K. pneumoniae isolates were obtained in this study. The results showed that K. Pneumonia isolates exhibited good sensitivity to amikacin (100%) and meropenem (80%). Sensitivity of levofloxacin (60%), ceftazidime (55%), ciprofloxacin (55%), cefoperazone (50%), and co-trimoxazole (50%) were moderate for the bacterial isolates. K. Pneumoniae isolates indicated low sensitivity to cefuroxime (45%), chloramphenicol (35%), cefepime (30%), cefotaxime (30%), tetracycline (30%), amoxicillin (5%), and ampicillin (5%). CONCLUSION: This study concludes that K. pneumoniae isolates are most sensitive to amikacin and less sensitive to ampicillin and amoxicillin.

Isolation and Characterization of Thermophilic Bacteria as Cellulolytic Enzyme Producer from the Hot Spring of Ie Seuum Aceh Besar, Indonesia

2020 ◽  
Vol 14 (1) ◽  
pp. 4
Author(s):  
RUHUL KHALILA ◽  
Lenni Fitri ◽  
SUHARTONO SUHARTONO
Keyword(s):  
Sampling Method ◽  
Bacterial Isolate ◽  
Hot Spring ◽  
Thermophilic Bacteria ◽  
Cellulolytic Enzyme ◽  
Biochemical Tests ◽  
Cellulase Enzymes ◽  
Isolation And Characterization

Cellulase enzymes can be isolated from thermophile bacteria obtained from the hot spring Ie Seuum, Aceh Besar. This research aimed to recover and characterize the isolates morphologically and biochemically followed by determination of the thermophile bacterial isolates potential as cellulolytic enzyme producers,. The sampling method in this research was conducted by a purposive sampling at temperature of 70 oC, 60 oC and 50 oC. Isolation of thermophilic bacteria was carried out on nutrient agar (NA) media. There were four isolates of thermophilic bacteria isolated recovered at 70 oC, five isolates at 60 oC, and seven isolates at 50 oC. Of the 18 isolates obtained, 15 of them were able to produce cellulase enzymes. Cellulase enzyme production can be determined by the presence of clear zones around bacterial colonies on CMC media after addition of 1% congo red drops and wash with 1 M NaCl. The highest five Cellulolytic Index (CI) values ​​were obtained from isolates ISB75; ISB64; ISB52; ISB54; ISB56 that were 1.23; 2.22; 1.39; 1.59; 1.10, respectively. Biochemical tests carried out on 5 isolates with the highest cellulolytic index values showed that the bacterial isolate were suspected to be from the genera of Bacillus sp.

scholarly journals Identification, Characterization, and Hydrolase Producing Performance of Thermophilic Bacteria: Geothermal Hot Springs in Eastern and Southeastern Anatolia Region of Turkey

2021 ◽  
Author(s):  
Orhan Uluçay ◽  
Arzu Görmez ◽  
Cem Öziç
Keyword(s):  
Hot Springs ◽  
Hot Spring ◽  
Thermophilic Bacteria ◽  
Hydrolytic Enzymes ◽  
Bacillus Coagulans ◽  
Rrna Gene ◽  
Bacterial Isolates ◽  
Thermophilic Bacilli ◽  
Geobacillus Kaustophilus ◽  
The Rich

Abstract In the last twenty years, researchers have increasingly focused on the rich microorganism-based diversity of natural hot spring resources to explore the benefits of thermophiles in industrial and biotechnological fields. For this purpose, a total of 83 thermophilic bacilli were isolated from 7 different geothermal hot springs (at temperatures ranging between 40 and 85 ◦ C) located in the east and Southeastern of Turkey. These isolates were identified by different methods such as physiological, morphological, biochemical, molecular properties. According to 16S rRNA gene sequence analysis, 5 different isolates ( Bacillus coagulans , Bacillus licheniformis , Bacillus subtilis , Bacillus thuringiensis , and Geobacillus kaustophilus ) were identified. It was observed that B. licheniformis and B. subtilis were the most species obtained from the researched hot spring sources. Phylogenetic relationships of isolates were evaluated with the help of a phylogenetic tree. The conditions of bacterial isolates to synthesize various hydrolytic enzymes such as protease, cellulase, lipase, and amylase were investigated. When the potential of isolates to produce hydrolytic enzymes was examined, protease 73 (88%), cellulase 34 (41%), lipase 69 (83%), and amylase 68 (82%) were detected. All isolates have at least one or more extracellular protease, cellulase, amylase, or lipase activity. Besides, 32.8% (27) of bacterial isolates were able to synthesize all of the hydrolytic enzymes.

Identification Pseudomonas aeruginosa by OprD Gene for Differentiation from Other Pseudomonas Species that Isolated from Clinical Samples

2019 ◽  
Vol 9 (01) ◽  
pp. 46-50
Author(s):  
Ashwak B Al-Hashimy ◽  
Huda S Alagely ◽  
Akeel K Albuaji ◽  
Khalid R Majeed
Keyword(s):  
Pseudomonas Aeruginosa ◽  
General Hospital ◽  
Culture Media ◽  
Final Diagnosis ◽  
Clinical Samples ◽  
Bacterial Isolates ◽  
Molecular Method ◽  
Biochemical Tests ◽  
Pseudomonas Species ◽  
Specific Sequences

The present study included the collection of 100 samples from various clinical sources for investigating the presence of P. aeruginosa in those sources, the samples have been collected from some hospitals in Baghdad and Hillah city (Al-qassim General Hospital, ,Al-hillah teaching hospital,and Al-hashimya General hospital ) which included wounds, burns, ear and sputum infections. The study was carried out through October 2017 till the end of March 2018. The samples were identified based on the morphological and microscopically characteristics of the colonies when they were culturing or number of culture media as well as biochemical tests, molecular identification were also used as a final diagnostic test for isolates that were positive as they belong to P.aeruginosa bacteria during previous tests based on the OprD gene which has specific sequences for P.aeruginosa bacteria as a detection gene and also consider as virulence factor so it have a synonyms mechanism to antibiotic resistance . The results of the final diagnosis showed that 38 isolates belong to target bacteria were distributed as 18 of burns, 11 isolates of wounds, 6 isolates of ear infection and 3 isolates of sputum, The examination of the sensitivity of all bacterial isolates was done for elected 38 isolation towards the 9 antibiotic by a Bauer - Kirby and the isolates were resistant for a number of antibiotics used such as Ciprofloxacin 65.7%, Norflaxacin 71%, Imipenem 63.1% Meropenem 68.4%, Gentamicin 65.7%, Amikacin 26.3%, Cefepime 68.4%, Ceftazidime 65.7% and Piperacillin 57.8%.Molecular method , All isolates (38) of P. aeruginosa positive for the diagnostic special gene (OprD) genes (100%).

scholarly journals Prevalence and antibiotic susceptibility of Aeromonas hydrophila isolated from freshwater fishes

2016 ◽  
Vol 4 (3) ◽  
pp. 411 ◽  
Author(s):  
Halima Sarder ◽  
Tahsin Khan ◽  
Mihir Lal Saha ◽  
Nusrat Jahan Punom ◽  
Shankar Chandra Mandal ◽  
...  
Keyword(s):  
Aeromonas Hydrophila ◽  
Antibiotic Susceptibility ◽  
Reference Strain ◽  
Bacterial Count ◽  
Freshwater Fishes ◽  
Total Bacterial Count ◽  
Bacterial Isolates ◽  
Biochemical Tests ◽  
Anabas Testudineus ◽  
Significant Difference

Aeromonas hydrophila is an opportunistic microorganism. It is a secondary biological agent that contributes to the occurrence of fish diseases and its deterioration. This research was undertaken to determine the prevalence of A. hydrophila in some freshwater fishes collected from three different fish markets of Dhaka City and to test their antibiotic susceptibility. Total bacterial count and total aeromonas on different aeromonas selective media were enumerated using serial dilution technique. Bacterial isolates were characterized to identify A. hydrophila using biochemical tests and with comparison to reference strain (ATCC 7966). The lowest Aeromonas count was detected to be 2.83±0.40×102 cfu/g in Anabas testudineus and the highest was 1.03±0.153×103 cfu/g in Oreochromis mossambicus. On market basis highest aeromonas count was found in Anando Bazar (8.10±1.09×102 cfu/g) and lowest in Hatirpool Bazar (5.63±0.90×102 cfu/g) with no significant difference. Maximum susceptibility to amikacin and gentamicin was observed whereas all of the isolates were found resistant to a commonly used antibiotic amoxycillin. The obtained results point that antimicrobial susceptibility was more or less similar regardless of the origin of the samples collected. All the fishes investigated in this study contained A. hydrophila in their different organs.

scholarly journals Detection of pathogenic bacteria associated with earphones used by students of Stamford University Bangladesh

2020 ◽  
Vol 10 (1) ◽  
pp. 1-4
Author(s):  
Omor Ahmed Chowdhury ◽  
Md Raihan Ahmed ◽  
Md Raihan Dipu ◽  
Md Aftab Uddin
Keyword(s):  
Pathogenic Bacteria ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Bacterial Isolates ◽  
Biochemical Tests ◽  
Disk Diffusion Method ◽  
Antibiotic Resistant ◽  
Potential Source ◽  
Different Types ◽  
Staphylococcus Spp

The use of earphones has increased in recent times throughout the world especially among the different level of students such as school, college or university who have a higher tendency of sharing these among them. Unlike airline headsets, headphones and stethoscope ear-pieces, ear phones are often shared by multiple users and can be a potential medium for transmission of pathogens, which can give rise to various ear related infections. The objective of this study was to detect the pathogenic bacteria from the ear-phones used by the students of Stamford University Bangladesh. A total of 16 ear-phone swabs were collected by sterile cotton swabs. The swabs were inoculated onto blood agar and incubated aerobically overnight at 37oC. Microscopic observation and standard biochemical tests were performed to confirm the identification of all the bacterial isolates. Six presumptively identified Staphylococcus spp. (38%) were tested against six different types of antibiotics following Kirby-Bauer disk diffusion method. Isolates were found to be 84% resistant against Cotrimoxazole and demonstrated 100% sensitivity to Vancomycin and Ciprorofloxacin. The findings of this study suggest the users to disinfect their respective ear phones and not to exchange them as they may act as a potential source to transfer pathogenic and antibiotic resistant bacteria among the ear phone users. Stamford Journal of Microbiology, Vol.10 (1) 2020: 1-4

scholarly journals KERAGAMAN BAKTERI PENAMBAT N PADA RHIZOSFIR TITONIA (Tithonia diversifolia) YANG TUMBUH PADA TANAH MASAM ULTISOL

Jurnal Solum ◽  
2012 ◽  
Vol 9 (2) ◽  
pp. 98
Author(s):  
Agustian Agustian ◽  
Rimadhani Syafei ◽  
Lusi Maira
Keyword(s):  
Incubation Temperature ◽  
Acid Soil ◽  
Growth Conditions ◽  
Stem Diameter ◽  
Optimum Growth ◽  
Tithonia Diversifolia ◽  
Bacterial Isolates ◽  
Optimum Range ◽  
Plant Soil ◽  
Soil Rhizosphere

Research on biodiversity of  N-fix bacteria was performed on rhizosphere  of Tithonia diversifolia grown at acid soil Ultisol. This study aimed to determine the biodiversity and populations of N-fix bacteria along with the growth rate of Tithonia and characterized the bacterial isolates obtained from the rhizosphere of this plant. Soil rhizosphere samples were taken from rhizospheres of Tithonia with different criteria of stem diameter i.e Ø <3 cm, and 3 to 6 cm that grown  at Faculty of Agriculture Andalas University experimental station.From these results it can be concluded that the diverse and larger population were  found in Tithonia with 3 to 6 cm stem diameter  an average of 19.7 x 103 cfu per g of soil. N-fix bacterial isolates obtained have a round, slimy, slippery and convex colonies and gram variable. Based on the color of their colonies, N-fix bacterial isolates obtained were classified into 3 groups with the following characteristics: (1) white milk isolates (A1ps, a2ps, B3ps), flourescent white and yellow, have flagella and produce auxin, (2) yellow isolate (B2K and B3K), with yellow flourescent, have flagella and produce auxin, and (3) the clear isolates that could separated into two groups i.e the flourescent group and produce auxin and has flagella isolates (A2b, A3b, and B2b) and non flourescent group, no flagella and does not produce auxin isolates (B1b, B3B). The optimum growth conditions for the all isolates were pH media nearly 7 with 35o C incubation temperature. The translucent isolates (A3b and B3B) have a optimum range pH from 4.36 to 6.17, while isolates with a yellow colonies (B2K) has a range of incubation temperature 25oC to 35oC. However, from the characterization performed could not permit to specify the isolates obtained into species.Key words : Biodiversity, N-fix bacteria, rhizosphere, Tithonia diversifolia

scholarly journals Detection of Polymorphism in the Gene blaKPC2 of Local Klebsiella pneumoniae Isolated from Iraqi Patients

2017 ◽  
Vol 11 (1) ◽  
pp. 26-33
Author(s):  
Saadi Abd-Alkareem Jasim ◽  
Mohammed Maaroof ◽  
Najwa Ahmed
Keyword(s):  
Environmental Management ◽  
Klebsiella Pneumoniae ◽  
Dna Sequences ◽  
Microscopic Examination ◽  
Gene Bank ◽  
Bacterial Isolates ◽  
Wild Type ◽  
Biochemical Tests ◽  
Specific Primers ◽  
Severe Infections

Carbapenemases are clinically important because they destroy and may confer a resistance to carbapenems, severe infections caused by carbapenemase producers is associated with increased mortality. To achieve this goal, 180 samples were collected from different clinical sources included 92 urine, 33 smears of wounds, 13 smears of burns and 42 sputum. The samples were taken from patients attended Al-Yarmouk Teaching Hospital and Ibin Baladi Hospital in Baghdad Governorate. Diagnosis of bacterial isolates was done depending upon the microscopic examination, the cultured characteristics and biochemical tests. DNA extracted from 84 samples. Accordingly, detection of blaKPC2 gene was conducted by using specific primers for amplification of blaKPC2 gene. Moreover, the sequencing of 910 bp for blaKPC2 gene was performed by the biotechnology lab. at the National Instrumentation Center for Environmental Management (NICEM). Such test has been implanted by using 3730XL as a DNA sequences. The obtained results were analyzed by blast at the National Center Biotechnology Information (NCBI) and detect polymorphism in blaKPC2 based on the Bio Edit. Consequently, 94 variations between 47 transversions, 43 transitions and 4 deletions nucleotide were noticed. In a sense the test showed 79% under sequence ID gb|CP009872.1| from 3037673 -3038166 number of nucleotide from K. pneumoniae subsp. pneumoniae strain KPNIH30 of Gene Bank, score (329) and expect 5e-86 with the wild type of blaKPC2 gene from Gene Bank. Finally, the results illustrated polymorphism between local strains of

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