scholarly journals Prevalence and antibiotic susceptibility of Aeromonas hydrophila isolated from freshwater fishes

2016 ◽  
Vol 4 (3) ◽  
pp. 411 ◽  
Author(s):  
Halima Sarder ◽  
Tahsin Khan ◽  
Mihir Lal Saha ◽  
Nusrat Jahan Punom ◽  
Shankar Chandra Mandal ◽  
...  
Keyword(s):  
Aeromonas Hydrophila ◽  
Antibiotic Susceptibility ◽  
Reference Strain ◽  
Bacterial Count ◽  
Freshwater Fishes ◽  
Total Bacterial Count ◽  
Bacterial Isolates ◽  
Biochemical Tests ◽  
Anabas Testudineus ◽  
Significant Difference

Aeromonas hydrophila is an opportunistic microorganism. It is a secondary biological agent that contributes to the occurrence of fish diseases and its deterioration. This research was undertaken to determine the prevalence of A. hydrophila in some freshwater fishes collected from three different fish markets of Dhaka City and to test their antibiotic susceptibility. Total bacterial count and total aeromonas on different aeromonas selective media were enumerated using serial dilution technique. Bacterial isolates were characterized to identify A. hydrophila using biochemical tests and with comparison to reference strain (ATCC 7966). The lowest Aeromonas count was detected to be 2.83±0.40×102 cfu/g in Anabas testudineus and the highest was 1.03±0.153×103 cfu/g in Oreochromis mossambicus. On market basis highest aeromonas count was found in Anando Bazar (8.10±1.09×102 cfu/g) and lowest in Hatirpool Bazar (5.63±0.90×102 cfu/g) with no significant difference. Maximum susceptibility to amikacin and gentamicin was observed whereas all of the isolates were found resistant to a commonly used antibiotic amoxycillin. The obtained results point that antimicrobial susceptibility was more or less similar regardless of the origin of the samples collected. All the fishes investigated in this study contained A. hydrophila in their different organs.

scholarly journals Pengaruh pemberian mikrokapsul probiotik Bacillus cereus P22 dan Staphylococcus lentus L1k pada pakan terhadap kinerja pertumbuhan, respons imun, dan resistensi ikan lele, Clarias gariepinus Burchell 1822 yang diinfeksi Aeromonas hydrophila

2018 ◽  
Vol 17 (2) ◽  
pp. 143
Author(s):  
Lilik Setiyaningsih ◽  
Widanarni Widanarni ◽  
Angela Mariana Lusiastuti3 ◽  
Munti Yuhana
Keyword(s):  
Bacillus Cereus ◽  
Growth Performance ◽  
Aeromonas Hydrophila ◽  
Clarias Gariepinus ◽  
Bacterial Count ◽  
Negative Control ◽  
Total Bacterial Count ◽  
African Catfish ◽  
Feed Conversion ◽  
Significant Difference

The aimed of this research was to evaluate the effects of dietary probiotic microcapsules B.cereus P22 and S. lentus (L1k) at different dose and frequency on growth performance, immune response and resistance of African catfish infected with A. Hydrophila. Probiotics used in this study were B. cereus P22 and S. lentus L1k encapsulated by spray draying method. The research was carried out for 56 days with eight treatments and four replications. The treatments were K- (negative control), K+ (positive control), A (feed supplemented with 0,5% of microencapsulated probiotic, fedevery day), B (feed supplemented with 0,5% of microencapsulated probiotic, fed once every three days), C (feed supplemented with 1% of microencapsulated probiotic, fed every day), D (feed supplemented with 1% of microencap-sulated probiotic with an administration once every three days), E (feed with 2% of microencapsulated probiotic with an administration every day) and F (feed with 2% of microencapsulated probiotic with an administration once every three days). On day 42, all of the fish except K- were challenged by intramuscular injection of A. hydrophila (108 CFU ml-1). In 40 days after infection, there were no significant difference on survival rate (SR) between treatments (p> 0.05). Treatment E displayed the higher growth rate (4,54±0,02%), total probiotic B. cereus (P22) and S . lentus (L1k) (4.06± 0.09 log CFU g-1; 4.02±0.08 log CFU g-1) than other treatments; whereas treatment D showed the best feed conversion rasio(1.191±0.013), and treatment F offered the highest total bacterial count (7.11±0.53 log CFU g-1). An administration of 2% microencapsulated probiotic in every day frequency demonstrated the better growth performance, immune res-ponse and resistance of African catfish to A. hydrophila compare with other treatments. AbstrakPenelitian ini bertujuan untuk mengkaji dosis dan frekuensi pemberian mikrokapsul probiotik melalui pakan terhadap kinerja pertumbuhan, respons imun, dan resistensi ikan lele yang diinfeksi Aeromonas hydrophila. Penelitian ini meng-gunakan Bacillus cereus P22 dan Staphylococcus lentus L1k yang telah dienkapsulasi melalui metode spray draying. Penelitian ini dilaksankan selama 56 hari dengan delapan perlakuan dan empat ulangan, terdiri atas perlakuan K- (kon-trol negatif), K+ (kontrol positif), A (pakan+mikrokapsul probiotik dosis 0,5% frekuensi setiap hari), B (pakan+mikro-kapsul probiotik dosis 0,5% frekuensi tiga hari sekali), C (pakan+mikrokapsul probiotik dosis 1% frekuensi setiap hari), D (pakan+mikrokapsul probiotik dosis 1% frekuensi tiga hari sekali), E (pakan+mikrokapsul probiotik dosis 2% fre-kuensi setiap hari) dan F (pakan+mikrokapsul probiotik dosis 2% frekuensi tiga hari sekali). Ikan diuji tantang dengan A. hydrophila pada hari ke 42 dengan kepadatan 108 CFU ml-1 secara intramuscular (kecuali K- diinjeksi dengan phos-phate buffer saline). Setelah 40 hari pascainjeksi, laju sintasan tidak menunjukkan perbedaan yang signifikan antarperlakuan (p>0,05).Perlakuan E menunjukkan laju pertumbuhan (4,54±0,02%) dan total probiotik B. cereus (P22) dan S. lentus (L1k) (4,06±0,09 log CFU g-1; 4,02±0,08 log CFU g-1) tertinggi; sementara perlakuan D memberikan hasil nisbah konversi pakan terbaik (1,191±0,013), perlakuan F menunjukkan total bacterial count tertinggi (7,11±0,53 log CFU g-1). Hasil penelitian menunjukkan bahwa dosis 2% yang diberikan setiap hari memberikan hasil yang lebih baik dalam meningkatkan laju pertumbuhan, respons imun, dan resistensi ikan lele terhadap A. hydrophila. 

scholarly journals Antibiogram of Bacteria Isolated from Tympanotonus fuscatus Var. Radula (Prosobranchia:Potamididae) Sold in Markets in Nasarawa State, Nigeria

2020 ◽  
pp. 1-9
Author(s):  
U. K. Asemota ◽  
M. D. Makut ◽  
S. O. Obiekezie ◽  
J. E. Owuna ◽  
M. O. Adamu
Keyword(s):  
Escherichia Coli ◽  
Bacterial Load ◽  
Bacterial Count ◽  
Total Bacterial Count ◽  
Bacterial Isolates ◽  
Salmonella Spp ◽  
Pseudomonas Spp ◽  
Gram Positive Bacteria ◽  
Bacillus Spp ◽  
Gram Negative Organisms

The aim of this study was to determine the antibiogram of bacterial isolates from Tympanotonus fuscatus var. radula sold in markets in Nasarawa State. Nigeria. Samples of Tympanotonus fuscatus var. radula (periwinkles) were bought from soup ingredient sellers at different sale locations in Keffi, Masaka and Orange markets and were analyzed using standard bacteriological methods. The bacterial isolates were identified using morphological, cultural and biochemical techniques. The total bacteria count varied from 1.18–3.20 x 108 CFU/g for the raw samples while the total bacterial count for the boiled samples varied from 0–1.57 x 108 CFU/g. Periwinkle samples with shells from Masaka market had the highest bacterial load with a mean total bacterial count of 2.94 x 10⁸ CFU/g and mean total coliform count of 2.80 x 10⁶ CFU/g. Raw periwinkle samples with shells had a higher bacterial load than samples without shells. There was also a drastic reduction in the bacterial load in the periwinkle samples after boiling under laboratory conditions. The bacteria isolated were Bacillus spp. and Staphylococcus aureus were the Gram-positive bacteria isolated. Enterobacter spp., Escherichia coli, Salmonella spp., Pseudomonas spp., Serratia spp. and Proteus spp. The most frequently occurring gram positive bacteria was Escherichia coli with an isolation frequency of 6(24%), the least frequently occurring was Bacillus spp., 8(32)%. Antibiotic susceptibility test showed that all the gram negative organisms exhibited sensitivity to ciprofloxacin: Escherichia coli (32 mm), Enterobacter spp. (41.5 mm), Proteus spp. (40.0 mm), Salmonella spp. (37.0 mm), Serratia spp. (26.0 mm), Pseudomonas spp. (23.0 mm). All the gram negative organisms showed marked resistance to vancomycin: Escherichia coli (12.0 mm), Enterobacter spp. (10.0 mm), Proteus spp. (11.0 mm), Salmonella spp. (5.0 mm), Serratia spp. (10.0 mm) and Pseudomonas spp. (4.5 mm).

scholarly journals Load and Antibiotic Susceptibility Pattern of Microorganisms in Muscle Foods Sold in Akure, Southwest Nigeria

2019 ◽  
Author(s):  
R.S. Bodunde ◽  
C.O. Ogidi ◽  
B.J. Akinyele
Keyword(s):  
Antibiotic Susceptibility ◽  
Bacterial Count ◽  
Poultry Meat ◽  
Resistant Bacteria ◽  
Susceptibility Pattern ◽  
Biochemical Tests ◽  
Antibiotic Susceptibility Pattern ◽  
Muscle Foods ◽  
First Choice ◽  
Southwest Nigeria

Background: Muscle foods, notably red meat, poultry meat, and fish are the first choice of animal source food with adequate protein for human. The present study was undertaken to analyze the load and antibiotic susceptibility pattern of microorganisms in muscle foods sold in Akure, Southwest Nigeria. Methods: Hundred muscle food samples, including meat and fish were collected from different locations (A-E) of Akure, Nigeria and examined microbiologically using cultural techniques, biochemical tests, and analytical profile index. Antibiotic susceptibility patterns were also determined in isolated microorganisms from muscle foods against different antibiotics. Data were analyzed using SPSS software version 17.0. Results: The highest (p<0.05) total viable bacterial count (8.3×106 CFU/g) were obtained from pork, including with 6.0×105 CFU/g for Staphylococcus and 5.8×105 CFU/g for Salmonella-Shigella. Mackerel collected from location D (Kings market) had the highest (p<0.05) bacterial count of 9.97×105 CFU/g, followed by 8.57×105 CFU/g, and 7.03×105 CFU/g in locations C and E, respectively. Low fungal counts were recorded ranged from 1.0×102 to 1.30×102 spore forming unit/g. The highest (p<0.05) occurrence of 26.50% was observed for Escherichia coli. The isolated microorganisms displayed varying degree of resistance (33.3 to 100%) to commonly used antibiotics. Conclusion: The microorganisms found in muscle foods from Akure, Nigeria could be considered as result of poor hygiene of the retailers or handlers. Also, presence of the multidrug resistant bacteria in muscle foods distributed in this region could pose a serious risk factor to public health. 

scholarly journals Effect of use varying ratios from fat with minced meat on bacterial load

2010 ◽  
Vol 34 (2) ◽  
pp. 142-147
Author(s):  
Fouad Kamil Salum
Keyword(s):  
Bacterial Load ◽  
Bacterial Count ◽  
Total Bacterial Count ◽  
The Third ◽  
Significant Difference ◽  
The Mean ◽  
Minced Meat

This study designed to know the effect of use different ration from mixing between minced meat With fat on total bacterial count of meat ( 80) sample was examine through the period of this study (40 sample for minced meat and 40 sample for minced fat) collected from shops in Baghdad city. The results showed the means of bacterial count was rise for the group contained just minced meat without any mixing in means reach to 9.6x 10 cfu \ml follow it with second group which include only minced fat by count 5.5x10 cfu\ml, and the study showed also decrease in bacterial count for (3) mixing groups ( fat with meat ), so the bacteria count for the third group which included 5%, from fat 1.1x10 cfu\ml and 1.3x10 for each of fourth and fifth group which contain the percent of fat10%,20%0n respectively. Also the study shows presence of a significant difference in ( p<0.05) between different mixing group (meat with fat) from one side to two qroups which included meat and fat only. Also recorded significant difference and in the same level in the mean of bacterial count between the first group which contain minced meat only, and the second one which contain fat only, there is no significant difference in mean of bacterial count among varying mixing group from statistical side but record obvious and important decline hygienically in bacterial count for each group. The study showed the best mixing percent for fat with minced meat were 10%,20% respectively which recorded the less bacterial count in each of these two groups. Conclusion mix fat with meat in ratios (10% - 20%) to get the beast decreas in bacterial load of meat .

scholarly journals Identification and antibiotic susceptibility of bacterial microbiota of freshwater fish

10.5219/1063 ◽  
2019 ◽  
Vol 13 (1) ◽  
pp. 408-414
Author(s):  
Alina Klūga ◽  
Miroslava Kačániová ◽  
Margarita Terentjeva
Keyword(s):  
Antibiotic Susceptibility ◽  
Negative Impact ◽  
Bacterial Species ◽  
Bacterial Count ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Total Bacterial Count ◽  
Pseudomonas Spp ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Fish Samples

The fish meat is an essential part of human diet. However, fish may be contaminated with different microorganisms, including pathogens. Antimicrobial resistance of fish microbiota may facilitate the spread of resistant microorganisms causing serious consequences for human health. The aim of the present study was to detect bacterial contamination in fish gill, gut and skin and to determine antimicrobial susceptibility of the bacterial isolates. Rainbow trout (Oncorhynchus mykiss) and bream (Abramis bram) were obtained from the market in Jelgava city. Chub (Leuciscus cephalus), crucian carp (Carassius carassius) and tench (Tinca tinca) were collected from fishermen. Fish samples were examined for the total bacterial count (TBC), coliforms, Enterobacteriaceae, Pseudomonas spp. and Aeromonas spp. Testing was done in accordance with International Organization for Standardization (ISO) standards. Identification of all bacteria was accomplished with the Matrix Assisted Laser Desorption Ionization – Time of Flight Mass Spectrometry (MALDI-TOF MS) method. The disc diffusion method was used for the detection of antibiotic susceptibility of isolated bacterial species. TBC ranged from 2.70 to 7.00 log CFU.g-1, coliforms from 0 to 2.67 log CFU.g-1, Enterobacteriaceae from 0 to 2.85 log CFU.g-1. The highest contamination with Pseudomonas spp. and Aeromonas spp. was observed in chub gut samples with 1.60 log CFU.g-1 and 2.23 log CFU.g-1, respectively. Altogether, 16 microbial genera and 31 bacterial species were identified. The dominant bacterial species belonged to Pseudomonas spp. (54%) and Enterobacteriaceae. Pseudomonas spp. were resistant to ticarcillin, susceptibility to ciprofloxacin showed 88% of isolates. All Enterobacteriaceae isolates were susceptible to imipenem. The microbial quality of the fish was acceptable, but the presence of antibiotic resistant bacteria may further cause a negative impact on public health.

scholarly journals ISOLASI DAN IDENTIFIKASI BAKTERI Aeromonas sp. DARI LELE DUMBO (Clarias sp.) DI KABUPATEN NGAWI

2016 ◽  
Vol 18 (2) ◽  
pp. 55
Author(s):  
Sri Rejeki ◽  
Triyanto Triyanto ◽  
Murwantoko Murwantoko
Keyword(s):  
Freshwater Fish ◽  
Aeromonas Hydrophila ◽  
Clinical Signs ◽  
Freshwater Fishes ◽  
Skin Ulcer ◽  
African Catfish ◽  
Bacterial Isolates ◽  
Disease Symptoms ◽  
Lethal Dosage

African catfish (Clarias sp.) is one of important freshwater fish which prefer consumed by people due to many advantages. Aeromonas sp. bacteria are dangerous patogen for freshwater fishes. This objective of the research was to isolate, identify and determine pathogenicity of Aeromonas sp. bacteria from African catfish from District Ngawi. The samples of catfish with 20 - 25 cm length showing clinical signs disease were obtained from three sub districts. Bacteria were isolated from kidney and inoculated into GSP medium. Characterization and identification through morphology of bacterial colonies, cells and biochemical test. Postulate Koch was conducted to verify abaility to couse disease. Pathogenicity was analyzed by determination of value of Lethal Dosage-50 on catfish on 7 - 9 cm length. The result showed that the disease symptoms of fish infected by the bacteria were skin ulcer, abdominal swelling and kidney damage. Fifteen bacterial isolates were collected which five, six and four isolates were from Kecamatan Karang Jati, Geneng dan Paron.sub-district respectively. The result showed 12 isolates (GKJ1, GKJ3, GKJ4, GGN1, GGN2, GGN3, GGN4, GGN5, GGN6, GPR2, GPR3 and GPR4) were identified as Aeromonas hydrophila. Three isolates (GKJ2, GKJ5 and GPR1) were identified as A. salmonicida. Isolate of A. hydrophila GKJ1, GKJ4, GGN2, GGN5, GPR2 and GPR4 were virulent to African catfish with LD50 values of 1,55 x 105, 3,89 x 105, 7,24 x 105, 2,39 x 105, 6,61 x 104 and 1,95 x 104 cfu/fish.

scholarly journals A study of bacterial pathogens and antibiotic susceptibility patterns in chronic suppurative otitis media

2017 ◽  
Vol 132 (1) ◽  
pp. 41-45 ◽  
Author(s):  
M R Mofatteh ◽  
F Shahabian Moghaddam ◽  
M Yousefi ◽  
M H Namaei
Keyword(s):  
Otitis Media ◽  
Antibiotic Susceptibility ◽  
Chronic Suppurative Otitis Media ◽  
Bacterial Isolates ◽  
Suppurative Otitis Media ◽  
Microbiological Methods ◽  
Significant Difference ◽  
Antibiotic Susceptibility Patterns ◽  
Periodic Evaluation ◽  
Susceptibility Patterns

AbstractObjective:To assess the frequency of bacterial agents in chronic suppurative otitis media and the antibiotic susceptibility patterns of isolates among patients.Methods:A total of 185 patients clinically diagnosed with chronic suppurative otitis media were interviewed and middle-ear effusion samples were collected using sterile swabs. All bacterial isolates were identified by conventional microbiological methods. Antibiotic susceptibility patterns of the isolates were determined by Kirby–Bauer disc diffusion.Results:Staphylococci spp. (64.9 per cent) were the most prevalent bacteria isolated, followed by Klebsiella spp. (12.9 per cent) and Pseudomonas aeruginosa (10.3 per cent). The most effective antibiotic for treatment of bacterial chronic suppurative otitis media was ciprofloxacin. Statistical analysis showed no significant difference in bacterial infestations among chronic suppurative otitis media patients and the antimicrobial susceptibility patterns of the bacterial isolates based on gender and age (p > 0.05).Conclusion:Our findings highlight the importance of a continuous and periodic evaluation of the bacteriological profile and antibiotic susceptibility patterns in chronic suppurative otitis media patients for efficacious treatment of the infection.

Microbial Aspects of the Inuvik Sewage Lagoon

1986 ◽  
Vol 18 (2) ◽  
pp. 117-128 ◽  
Author(s):  
J. G. Henry ◽  
D. Prasad
Keyword(s):  
Bacterial Count ◽  
Total Alkalinity ◽  
Total Bacterial Count ◽  
Bacterial Number ◽  
Psychrotrophic Bacteria ◽  
Volatile Solids ◽  
Significant Difference ◽  
Order Of Magnitude ◽  
Plate Counts

A facultative sewage lagoon in Inuvik, N.W.T. was studied in August (ice free) and November (ice covered) 1984. Emphasis was placed on the microbiological aspects and their relation to lagoon performance. Analyses performed included, pH, total alkalinity, total solids, suspended solids, volatile solids, COD and BOD5 determinations. The microbiological tests included total plate counts (20°C) and psychrotrophic counts (1°C). Results indicated that the Inuvik sewage lagoon system achieved removal efficiencies, ranging from 90% BOD5 removal in summer to about 80% in winter. The total bacterial count was found to decrease in the liquid of the primary cells in the winter, but the bacterial count in the secondary cell was similar under both winter and summer conditions. No significant difference existed in bacterial counts in the sludge samples between primary and secondary cells. The total bacterial number decreased by one order of magnitude in the winter in sludges from all cells. A shift from mesophilic to psychrotrophic bacteria occurred in the winter. The percentage of psychrotrophs increased from less than 1% to over 80% with the change in season. The role of psychrotrophs in lagoon performance is discussed.

scholarly journals Microbial Incidence and Antibiotic susceptibility for Bacterial isolates in The Mobile Phone of Healthcare workers and University Employments in Basrah City

2020 ◽  
Vol 14 (3) ◽  
pp. 1863-1870
Author(s):  
Rana Mohammad Abd-ulnabi ◽  
Zuhair G. Alshaheen ◽  
Rafeef A. Abdul-jabbar
Keyword(s):  
Mobile Phone ◽  
Mobile Phones ◽  
Healthcare Workers ◽  
Pathogenic Bacteria ◽  
Bacterial Isolates ◽  
Biochemical Tests ◽  
Candida Spp ◽  
Significant Difference ◽  
Field Samples ◽  
Bacillus Spp

The mobile phones come to be an important device all people use it for communication and phones can be contaminated with bacterial isolates. this study tries to do a little investigation about kinds and percent of pathogenic bacteria and the detection of some antibiotic resistance one and difference between that found in the healthcare worker’s mobile phone and peoples who work out of that field. Samples collection were done using mobile phones belongs to healthcare workers and Basra University employments. Swabs were cultured on different media then the suspected colonies were identified by recognizing the morphology of colonies, staining with Gram’s stain then tested with biochemical tests. Our study shows bacterial isolates in all the screened mobile phone, The highest proportions were recorded by Bacillus spp (66%) and Pseudomonas spp (56%) while the lowest proportions were Staphylococcus aureus (8%) and Candida spp (12%). And there was a significant difference in the kinds and percent of bacterial isolates between the healthcare workers and employments mobile phones, besides the appearance of Cefotaxime and Cefatizoxime resistance Proteus sp. and Trimethoprim + Sulfamethoxazole resistance Klebsiella spp. also the intermediate susceptibility to the different antibiotic was shown in all isolates. All the mobile phones can be contaminated by various kinds of bacteria some of these were resistant to some antibiotics and there was a significant difference in the percent and kinds of bacterial isolates between the health care worker and people who work out of this field.

scholarly journals Antibiotic Susceptibility Profile of Enterobacteriaceae Isolates from a Locally Preferred Drink – Kunun-Zaki in Kaduna State

2021 ◽  
Vol 16 (2) ◽  
pp. 149-157
Author(s):  
S.K. Parom ◽  
G.D. Gamaleon ◽  
S.G. Ishaku ◽  
J.C. Igwe ◽  
O.F. Obajuluwa
Keyword(s):  
Antibiotic Susceptibility ◽  
Susceptibility Testing ◽  
Bacterial Isolates ◽  
Proteus Vulgaris ◽  
Biochemical Tests ◽  
Gram Staining ◽  
Potential Source ◽  
The People ◽  
Antibiotics Susceptibility ◽  
Potential Public Health

Background: ‘Kunun-zaki’ is a local drink made from slightly fermented corn or maize, with water as the medium. It is commonly taken by the people in the northern part of Nigeria. It has high nutritional benefit, even though it can also serve as a potential source of waterborne disease if not prepared under strict hygienic condition.Objective: This study was carried out to isolate Enterobacteriaceae bacteria from ‘kunun-zaki’ drinks and to determine their antibiotic susceptibility to commonly prescribed antibiotics within Kaduna metropolis of Kaduna State, Nigeria.Methods: A total of 20 samples were randomly collected from different locations (within a period of two weeks), analysed and coliform count determined. The bacterial isolates were identified using gram staining, selective media and standard biochemical tests. Antibiotics susceptibility testing was carried out using Kirby-Bauer agar diffusion method.Results: The following bacteria were isolated: Enterobacteriaceae (50%), Staphylococcus aureus (30%), Pseudomonas aeruginosa (20%). The Enterobacteriaceae bacteria isolated were: Klebsiella pneumoniae 4 (20%), Escherichia coli 4, (20%), Citrobacter freundii 1 (5%) and Proteus vulgaris 1 (5%). The antibiotic susceptibility testrevealed that ciprofloxacin, gentamicin, imipenem and ofloxacin (each 100%) chloramphenicol (90%), amoxicillinclavulanate (80%) and streptomycin (70%) were active against the isolates while the isolates were resistant to ceftazidime (100%), meropenem (80%), cefoxitin and ceftriaxone (70%).Conclusion: This study showed the presence of Enterobacteriaceae contaminants in locally consumed drink (kununzaki) sold within Kaduna metropolis which pose a potential public health risk including the risk of transmission of strains which can lead to the spread of antibiotic resistance in the society.

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