scholarly journals Susceptibility of Klebsiella Pneumoniae Isolated from Pus Specimens of Post-Surgery Patients in Medan, Indonesia to Selected Antibiotics

2019 ◽  
Vol 7 (22) ◽  
pp. 3861-3864 ◽  
Author(s):  
Popi Patilaya ◽  
Dadang Irfan Husori ◽  
Lany Marhafanny
Keyword(s):  
Klebsiella Pneumoniae ◽  
Klebsiella Pneumonia ◽  
Good Sensitivity ◽  
Bacterial Isolates ◽  
Inhibitory Zone ◽  
Biochemical Tests ◽  
Post Surgery ◽  
Low Sensitivity

AIM: This study was to determine the sensitivity of Klebsiella pneumonia isolated from pus specimens of post-surgery patients in Medan, Indonesia to selected antibiotics. METHODS: Samples were collected at the Laboratory of Microbiology, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia. The isolated bacteria were identified by Gram’s stain, colony characteristics, and biochemical tests. Susceptibility of K. pneumoniae isolates were tested to selected antibiotics including amikacin, meropenem, levofloxacin, ciprofloxacin, co-trimoxazole, ceftazidime, cefoperazone, cefuroxime, cefepime, cefotaxime, tetracycline, chloramphenicol, amoxicillin and ampicillin with Kirby Bauer method by measuring the inhibitory zone. RESULTS: A total of 20 K. pneumoniae isolates were obtained in this study. The results showed that K. Pneumonia isolates exhibited good sensitivity to amikacin (100%) and meropenem (80%). Sensitivity of levofloxacin (60%), ceftazidime (55%), ciprofloxacin (55%), cefoperazone (50%), and co-trimoxazole (50%) were moderate for the bacterial isolates. K. Pneumoniae isolates indicated low sensitivity to cefuroxime (45%), chloramphenicol (35%), cefepime (30%), cefotaxime (30%), tetracycline (30%), amoxicillin (5%), and ampicillin (5%). CONCLUSION: This study concludes that K. pneumoniae isolates are most sensitive to amikacin and less sensitive to ampicillin and amoxicillin.

scholarly journals Detection of Polymorphism in the Gene blaKPC2 of Local Klebsiella pneumoniae Isolated from Iraqi Patients

2017 ◽  
Vol 11 (1) ◽  
pp. 26-33
Author(s):  
Saadi Abd-Alkareem Jasim ◽  
Mohammed Maaroof ◽  
Najwa Ahmed
Keyword(s):  
Environmental Management ◽  
Klebsiella Pneumoniae ◽  
Dna Sequences ◽  
Microscopic Examination ◽  
Gene Bank ◽  
Bacterial Isolates ◽  
Wild Type ◽  
Biochemical Tests ◽  
Specific Primers ◽  
Severe Infections

Carbapenemases are clinically important because they destroy and may confer a resistance to carbapenems, severe infections caused by carbapenemase producers is associated with increased mortality. To achieve this goal, 180 samples were collected from different clinical sources included 92 urine, 33 smears of wounds, 13 smears of burns and 42 sputum. The samples were taken from patients attended Al-Yarmouk Teaching Hospital and Ibin Baladi Hospital in Baghdad Governorate. Diagnosis of bacterial isolates was done depending upon the microscopic examination, the cultured characteristics and biochemical tests. DNA extracted from 84 samples. Accordingly, detection of blaKPC2 gene was conducted by using specific primers for amplification of blaKPC2 gene. Moreover, the sequencing of 910 bp for blaKPC2 gene was performed by the biotechnology lab. at the National Instrumentation Center for Environmental Management (NICEM). Such test has been implanted by using 3730XL as a DNA sequences. The obtained results were analyzed by blast at the National Center Biotechnology Information (NCBI) and detect polymorphism in blaKPC2 based on the Bio Edit. Consequently, 94 variations between 47 transversions, 43 transitions and 4 deletions nucleotide were noticed. In a sense the test showed 79% under sequence ID gb|CP009872.1| from 3037673 -3038166 number of nucleotide from K. pneumoniae subsp. pneumoniae strain KPNIH30 of Gene Bank, score (329) and expect 5e-86 with the wild type of blaKPC2 gene from Gene Bank. Finally, the results illustrated polymorphism between local strains of

scholarly journals SKRINING BAKTERI TERMOFILIK POTENSIAL AMILOLITIK DARI SUMBER AIR PANAS WAY BELERANG KALIANDA LAMPUNG SELATAN

2021 ◽  
Vol 6 (1) ◽  
pp. 1-7
Author(s):  
Sefi Desfeni Mawati ◽  
Esti Harpen ◽  
Hilma Putri Fidyandini
Keyword(s):  
Incubation Temperature ◽  
Hot Spring ◽  
Thermophilic Bacteria ◽  
Pseudomonas Stutzeri ◽  
Optimum Temperature ◽  
Bacterial Isolates ◽  
Inhibitory Zone ◽  
Biochemical Tests ◽  
Amylolytic Enzyme ◽  
Thermostable Amylase

Thermophilic bacteria that produced amylase and protease have been isolated from Way Belerang hot spring, Kalianda, South Lampung. This research aims to screen and identify thermophilic bacteria that have the potential to produce thermostable amylase and protease enzymes.The research procedures included sampling, isolation of enzyme-producing thermophilic bacteria, a series of phenotypic and biochemical tests, and molecular identification by 16s rRNA. This study used 2 treatments, namely incubation temperature 37 and 50 ºC with 3 repetitions. The results showed that the optimum temperature for growth of thermophilic bacterial isolates and thermophilic bacterial isolates producing amylase enzymes was 50ºC. The bacteria isolate that had the best amylolytic enzyme activity was Isolate A.WB.50.1 with a diameter of the inhibitory zone was 15.44 mm. Isolate A.WB.50.1 has been identified by the species Pseudomonas stutzeri.

scholarly journals Frequency of bacterial isolates and antibiotics resistance patterns in urine and pus samples.

2021 ◽  
Vol 28 (11) ◽  
pp. 1571-1577
Author(s):  
Sajid Ali ◽  
Hina Khalid ◽  
Noor Muhammad ◽  
Syed Luqman Shuaib ◽  
Anees Muhammad
Keyword(s):  
Culture Media ◽  
Cross Sectional Study ◽  
Urine Samples ◽  
Antibiotics Resistance ◽  
Klebsiella Pneumonia ◽  
Bacterial Isolates ◽  
Biochemical Tests ◽  
Cross Sectional ◽  
E Coli ◽  
Antibiotic Resistance Profile

Objectives: The present study is aimed to determine frequency of bacteria in urine and pus sample along with the antibiotic resistance profile of isolated bacteria. Study Design: Cross-Sectional Study. Setting: Real-Time PCR Laboratory at Dabgari Garden, Peshawar with the Collaboration of Health Biotechnology Laboratory, Department of Biotechnology, Abdul Wali Khan University, Mardan. Period: June 2018 to July 2019. Material & Methods: The urine and pus samples were collected from suspected patients and were cultured on appropriate culture media. The biochemical tests were also performed after growing on culture plates for identification and confirmation of bacterial isolates. The disc diffusion technique was used to evaluate the antibiotic pattern of retrieved isolates. Result: A total of 525 samples of pus and urine samples were collected from different regions of Peshawar. Out of the total, 237 (45.1%) samples were found positive for bacterial growth whereas the remaining (54.9%) were observed negative. Among total positive isolates, 220 (92.8%) were from urine samples, and 17 (7.2%) were from pus samples. The predominant isolate was Escherichia coli (E. coli) (90.3%) retrieved from positive samples, followed by Pseudomonas aeruginosa (7.2%), Klebsiella pneumonia (1.3%), and Staphylococcus aureus (1.3%). The most effective antibiotic was Fosfomycin against bacteria whereas Nalidixic acid, Nitrofurantoin, and Amoxicillin/clavulanic acid were found less effective against bacterial isolates. Conclusion: Most frequent bacteria isolated was E. coli and the most efficient drug was Fosmomycin and the least was Nitrofurantoin, and Amoxicillin.

scholarly journals Antibiotic resistance pattern and frequency of some beta lactamase genes in Klebsiella pneumoniae isolated from raw milk samples in Iran

2021 ◽  
Vol 71 (4) ◽  
pp. 2455
Author(s):  
E. ENFERAD ◽  
S. MAHDAVI
Keyword(s):  
Antibiotic Resistance ◽  
Klebsiella Pneumoniae ◽  
Raw Milk ◽  
Cow Milk ◽  
Resistance Pattern ◽  
Klebsiella Pneumonia ◽  
Beta Lactamase ◽  
Biochemical Tests ◽  
Milk Samples ◽  
Antibiotic Resistance Pattern

Klebsiella pneumoniae have become an important cause of mastitis in dairy cows. Resistance to beta lactam antibiotics resulted from beta lactamases enzyme production. The aim of this study was to investigate the antibiotic resistance pattern and frequency of some beta lactamase genes in Klebsiella pneumoniae isolated from raw milk samples in Iran. 200 raw cow milk samples were collected from different villages of north west of Iran. The samples were cultured and biochemical tests were performed for phenotypic diagnosis. Then, antibiotic resistance pattern was determined by antibiogram test. Finally, the presence of CTX, SHV and TEM genes in Klebsiella pneumoniae isolates was found by PCR method. Of total 200 raw cow milk samples, 80 samples (40%) contained Klebsiella pneumoniae. The frequency of CTX, SHV and TEM genes in Klebsiella pneumoniae isolates was 50 (62.5%), 34 (42.5%) and 70 (87.5%), respectively. 14 Klebsiella pneumoniae isolates (17.5%) possessed all three intended genes simultaneously. All strains of Klebsiella pneumoniae (100%) were resistant to ampicillin. The most strains were resistant to ceftriaxone (75%), gentamicin (70%) and nitrofurantoin (70%). 4 Klebsiella pneumoniae strains (5%) were resistant to all of tested antibiotics. The results showed high frequency of ESBLs and antibiotic resistance in Klebsiella pneumonia samples isolated from raw milk. It may occur exchange of resistance genes within and across species and with commensal bacteria of the human and animals.

scholarly journals Investigation of the presence of NDM-1, VIM-1 and OXA-1 genes in MDR strains of Klebsiella pneumoniae isolated from clinical specimens

2019 ◽  
Author(s):  
Melika Taleb ◽  
Farzaneh Hosseini
Keyword(s):  
Klebsiella Pneumoniae ◽  
Opportunistic Pathogen ◽  
New Drugs ◽  
Diffusion Method ◽  
Klebsiella Pneumonia ◽  
Beta Lactam ◽  
Biochemical Tests ◽  
Disk Diffusion Method ◽  
Enzyme Gene

Abstract Background:Klebsiella pneumoniae has emerged as a significant opportunistic pathogen responsible for nosocomial infections. This bacteria is frequently resistant to multiple classes of antibiotics such as the family of beta-lactam antibiotics. The aim of this study was the molecular evaluation of resistant strains of Klebsiella pneumonia isolation of multiple resistant drugs from clinical specimens.Methods:60 clinical isolates of Klebsiella pneumoniae were isolated and identified by staining and biochemical tests during fall season from a laboratory in Tehran. Antimicrobial susceptibility testing was carried out using a disk diffusion method with CLSI standards. All isolates were examined by PCR test for the presence of NDM-1, VIM-1, and OXA-1 genes.Results:The highest resistance in the strains was related to antibiotic ampicillin (90%), followed by the highest resistance to ciprofloxacin (70%), cefixime (66.66%), trimethoprim-sulfamethoxazole (65%), piperacillin (50%). Also, most susceptibilities were to ampicillin antibiotics (85%), gentamicin (70%), piperacillin-tazobactam (60%) and amikacin (56.66%). Out of the 60 isolated strains, 3 isolates (5%) had the VIM-1 enzyme gene, 2 isolates (3.3%) had the OXA-1 enzyme gene and no isolates were found with the NDM-1 enzyme gene. Conclusion:The high levels of antibiotic resistance among the organisms that cause clinical infections suggest that the mechanisms involved in the development of resistance and the evaluation of antimicrobial activity of new drugs in vitro can help the process of effective treatment of these infections.

Identification Pseudomonas aeruginosa by OprD Gene for Differentiation from Other Pseudomonas Species that Isolated from Clinical Samples

2019 ◽  
Vol 9 (01) ◽  
pp. 46-50
Author(s):  
Ashwak B Al-Hashimy ◽  
Huda S Alagely ◽  
Akeel K Albuaji ◽  
Khalid R Majeed
Keyword(s):  
Pseudomonas Aeruginosa ◽  
General Hospital ◽  
Culture Media ◽  
Final Diagnosis ◽  
Clinical Samples ◽  
Bacterial Isolates ◽  
Molecular Method ◽  
Biochemical Tests ◽  
Pseudomonas Species ◽  
Specific Sequences

The present study included the collection of 100 samples from various clinical sources for investigating the presence of P. aeruginosa in those sources, the samples have been collected from some hospitals in Baghdad and Hillah city (Al-qassim General Hospital, ,Al-hillah teaching hospital,and Al-hashimya General hospital ) which included wounds, burns, ear and sputum infections. The study was carried out through October 2017 till the end of March 2018. The samples were identified based on the morphological and microscopically characteristics of the colonies when they were culturing or number of culture media as well as biochemical tests, molecular identification were also used as a final diagnostic test for isolates that were positive as they belong to P.aeruginosa bacteria during previous tests based on the OprD gene which has specific sequences for P.aeruginosa bacteria as a detection gene and also consider as virulence factor so it have a synonyms mechanism to antibiotic resistance . The results of the final diagnosis showed that 38 isolates belong to target bacteria were distributed as 18 of burns, 11 isolates of wounds, 6 isolates of ear infection and 3 isolates of sputum, The examination of the sensitivity of all bacterial isolates was done for elected 38 isolation towards the 9 antibiotic by a Bauer - Kirby and the isolates were resistant for a number of antibiotics used such as Ciprofloxacin 65.7%, Norflaxacin 71%, Imipenem 63.1% Meropenem 68.4%, Gentamicin 65.7%, Amikacin 26.3%, Cefepime 68.4%, Ceftazidime 65.7% and Piperacillin 57.8%.Molecular method , All isolates (38) of P. aeruginosa positive for the diagnostic special gene (OprD) genes (100%).

scholarly journals Klebsiella pneumoniae do Serotipo K1 e do Clone Hipervirulento ST23: Primeiro Caso Documentado em Portugal

2017 ◽  
Vol 30 (6) ◽  
pp. 496 ◽  
Author(s):  
Aida Pereira ◽  
Tiago Petrucci ◽  
Maria João Simões
Keyword(s):  
Klebsiella Pneumoniae ◽  
Clinical Cure ◽  
Virulent Strain ◽  
Pyogenic Liver Abscess ◽  
Caucasian Woman ◽  
Klebsiella Pneumonia ◽  
Asian Countries ◽  
Catheter Drainage ◽  
Epidemiological Risk ◽  
Old Portuguese

The hypervirulent K1 serotype Klebsiella pneumoniae is responsible for a new invasive syndrome, typically associated to hepatic abscesses with extra-hepatic complications. Initially described in Taiwan, it has significantly spread to several Asian countries and more recently to Europe and North America, thus constituting an emerging and global problem. The authors describe a case report of a 64-years-old portuguese caucasian woman without any previous diseases or epidemiological risk factors such as trips or contact with Asian products or population, diagnosed with a pyogenic liver abscess with pleural effusion caused by this hyper-virulent strain. A successful clinical cure was achieved after the etiological identification and treatment with antimicrobial therapy combined with catheter drainage. This is the first identification of hypervirulent Klebsiella pneumonia ST 23 clone in Portugal in the context of an invasive syndrome.

scholarly journals Comparison of the Superpolymyxin and ChromID Colistin R Screening Media for the Detection of Colistin-Resistant Enterobacteriaceae from Spiked Rectal Swabs

2018 ◽  
Vol 63 (1) ◽  
Author(s):  
Delphine Girlich ◽  
Thierry Naas ◽  
Laurent Dortet
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
Confidence Interval ◽  
Enterobacter Cloacae ◽  
Bacterial Isolates ◽  
Colistin Resistance ◽  
Gram Negative ◽  
Content Type ◽  
Rectal Swabs ◽  
Stool Samples

ABSTRACT The dissemination of carbapenemase-producing Enterobacteriaceae (CPE) has led to the increased use of colistin, which has resulted in the emergence of colistin-resistant Enterobacteriaceae worldwide. One of the most threatening scenarios is the dissemination of colistin resistance in CPE, particularly the plasmid-encoded resistance element MCR. Thus, it has now become mandatory to possess reliable media to screen for colistin-resistant Gram-negative bacterial isolates, especially Enterobacteriaceae. In this study, we evaluated the performances of the Superpolymyxin medium (ELITechGroup) and the ChromID Colistin R medium (bioMérieux) to screen for colistin-resistant Enterobacteriaceae from spiked rectal swabs. Stool samples were spiked with a total of 94 enterobacterial isolates (Escherichia coli, Klebsiella pneumoniae, Salmonella enterica, Enterobacter cloacae), including 53 colistin-resistant isolates. ESwabs (Copan Diagnostics) were then inoculated with those spiked fecal suspensions, and culture proceeded as recommended by both manufacturers. The sensitivity of detection of colistin-resistant Enterobacteriaceae was 86.8% (95% confidence interval [95% CI] = 74.0% to 94.0%) using both the Superpolymyxin medium and the ChromID Colistin R plates. Surprisingly, the isolates that were not detected were not the same for both media. The specificities were high for both media, at 97.9% (95% CI = 87.3% to 99.9%) for the Superpolymyxin medium and 100% (95% CI = 90.4% to 100%) for the ChromID Colistin R medium. Both commercially available media, ChromID Colistin R and Superpolymyxin, provide useful tools to screen for colistin-resistant Enterobacteriaceae from patient samples (rectal swabs) regardless of the level and mechanism of colistin resistance.

scholarly journals Prevalence and antibiotic susceptibility of Aeromonas hydrophila isolated from freshwater fishes

2016 ◽  
Vol 4 (3) ◽  
pp. 411 ◽  
Author(s):  
Halima Sarder ◽  
Tahsin Khan ◽  
Mihir Lal Saha ◽  
Nusrat Jahan Punom ◽  
Shankar Chandra Mandal ◽  
...  
Keyword(s):  
Aeromonas Hydrophila ◽  
Antibiotic Susceptibility ◽  
Reference Strain ◽  
Bacterial Count ◽  
Freshwater Fishes ◽  
Total Bacterial Count ◽  
Bacterial Isolates ◽  
Biochemical Tests ◽  
Anabas Testudineus ◽  
Significant Difference

Aeromonas hydrophila is an opportunistic microorganism. It is a secondary biological agent that contributes to the occurrence of fish diseases and its deterioration. This research was undertaken to determine the prevalence of A. hydrophila in some freshwater fishes collected from three different fish markets of Dhaka City and to test their antibiotic susceptibility. Total bacterial count and total aeromonas on different aeromonas selective media were enumerated using serial dilution technique. Bacterial isolates were characterized to identify A. hydrophila using biochemical tests and with comparison to reference strain (ATCC 7966). The lowest Aeromonas count was detected to be 2.83±0.40×102 cfu/g in Anabas testudineus and the highest was 1.03±0.153×103 cfu/g in Oreochromis mossambicus. On market basis highest aeromonas count was found in Anando Bazar (8.10±1.09×102 cfu/g) and lowest in Hatirpool Bazar (5.63±0.90×102 cfu/g) with no significant difference. Maximum susceptibility to amikacin and gentamicin was observed whereas all of the isolates were found resistant to a commonly used antibiotic amoxycillin. The obtained results point that antimicrobial susceptibility was more or less similar regardless of the origin of the samples collected. All the fishes investigated in this study contained A. hydrophila in their different organs.

scholarly journals Detection of pathogenic bacteria associated with earphones used by students of Stamford University Bangladesh

2020 ◽  
Vol 10 (1) ◽  
pp. 1-4
Author(s):  
Omor Ahmed Chowdhury ◽  
Md Raihan Ahmed ◽  
Md Raihan Dipu ◽  
Md Aftab Uddin
Keyword(s):  
Pathogenic Bacteria ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Bacterial Isolates ◽  
Biochemical Tests ◽  
Disk Diffusion Method ◽  
Antibiotic Resistant ◽  
Potential Source ◽  
Different Types ◽  
Staphylococcus Spp

The use of earphones has increased in recent times throughout the world especially among the different level of students such as school, college or university who have a higher tendency of sharing these among them. Unlike airline headsets, headphones and stethoscope ear-pieces, ear phones are often shared by multiple users and can be a potential medium for transmission of pathogens, which can give rise to various ear related infections. The objective of this study was to detect the pathogenic bacteria from the ear-phones used by the students of Stamford University Bangladesh. A total of 16 ear-phone swabs were collected by sterile cotton swabs. The swabs were inoculated onto blood agar and incubated aerobically overnight at 37oC. Microscopic observation and standard biochemical tests were performed to confirm the identification of all the bacterial isolates. Six presumptively identified Staphylococcus spp. (38%) were tested against six different types of antibiotics following Kirby-Bauer disk diffusion method. Isolates were found to be 84% resistant against Cotrimoxazole and demonstrated 100% sensitivity to Vancomycin and Ciprorofloxacin. The findings of this study suggest the users to disinfect their respective ear phones and not to exchange them as they may act as a potential source to transfer pathogenic and antibiotic resistant bacteria among the ear phone users. Stamford Journal of Microbiology, Vol.10 (1) 2020: 1-4

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