scholarly journals Antimicrobial Activity of Ethanolic Extract of Sempur (Dillenia suffruticosa (Griff.) Martelli) Leaves against Pathogenic Microorganisms

2021 ◽  
Vol 4 (2) ◽  
pp. 135-144
Author(s):  
Vilya Syafriana ◽  
Amelia Febriani ◽  
Suyatno Suyatno ◽  
Nurfitri Nurfitri ◽  
Fathin Hamida
Keyword(s):  
Antimicrobial Activity ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Antimicrobial Test ◽  
The People ◽  
Phytochemical Constituents ◽  
Boiled Water

Sempur (Dillenia suffruticosa) leaves are known as a traditional medicine for the people of Bangka-Belitung Island. The local people empirically utilize the boiled water of D. suffruticosa leaves as anti-diarrhea. However, the antimicrobial activity of the ethanol extract of D. suffruticosa leaves has not been reported. This study aims to determine the antimicrobial activity of the ethanol extract of D. suffruticosa leaves against several microorganisms: Staphylococcus aureus as Gram-positive bacteria, Escherichia coli as Gram-negative bacteria, and Candida albicans as fungi. Extraction was carried out by maceration method with 70% ethanol, then screened for phytochemical constituents. The antimicrobial test was carried out by the disc diffusion method using Nutrient Agar (NA) for bacteria, and Sabouraud Dextrose Agar (SDA) for fungi. The results of phytochemical screening showed that the ethanol extract of D. suffruticosa leaves contained alkaloids, flavonoids, tannins, and saponins. The antimicrobial test showed that the extract of D. suffruticosa leaves could inhibit the growth of S. aureus at concentrations of 10%, 20%, and 40% were 8.35±0.05; 9.34±0.32; and 10.52±0.22, respectively. The ethanol extract of D. suffruticosa leaves could inhibit the growth of S. aureus, whereas E. coli and C. albicans did not show any activity.

scholarly journals PRELIMINARY PHYTOCHEMICAL SCREENING AND IN VITRO ANTI-MICROBIAL ACTIVITY OF ETHANOLIC EXTRACT OF DELONIX REGIA (HOOK) RAF. BARK (FAMILY-LEGUMINOSAE)

2018 ◽  
Vol 10 (4) ◽  
pp. 43
Author(s):  
Bikash Gupta ◽  
Biswajit Dash ◽  
Sujata Paul ◽  
Aditya Bora
Keyword(s):  
Antimicrobial Activity ◽  
Microbial Activity ◽  
Ethanolic Extract ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Delonix Regia ◽  
Gram Positive Bacteria ◽  
Phytochemical Constituents ◽  
Anti Fungal

Objective: To estimate the anti-microbial activity of ethanolic extract of Delonix regia (Hook.) Raf (family-Leguminosae) in association with phytochemical analysis.Methods: The ethanolic extract of the bark of Delonix regia (Hook.) Raf was prepared and investigate for phytochemical constituents using standard methods. Antimicrobial activity of ethanolic extract of were carried out against one Gram positive bacteria= Staphylococcus aureus and one Gram negative bacteria= Escherichia coli. The anti-fungal activity of the plant extract was evaluated on Candida albicans. The testing was done by the disc diffusion method. A zone of inhibition of ethanolic extract was compared with that of standard Amikacin for anti-bacterial activities and Fluconazole for anti-fungal activity.Results: The present investigation shows the phytochemical analysis, antimicrobial activity of the ethanolic extract of the bark of Delonix regia (Hook.) Raf. Various phytochemical analyses revealed the presence of alkaloids, anthraquinone, phenol, terpenes, cardenolides, phlobatannins, tannin, chalcones, steroids. The anti-microbial activity of the ethanolic extract of the plant showed significant results against all three of the test organisms.Conclusion: The present study concluded that ethanolic extract of the bark of Delonix regia (Hook.) Raf contains the high existence of phytochemicals. The ethanolic extract of the plant was found to possess promising antimicrobial activity when compared with the standards.

Antioxidant and Antimicrobial Activities of the Fresh aerial parts of Ammi visnaga L. from Algeria

2016 ◽  
Vol 6 (2) ◽  
pp. 70-76
Author(s):  
Soumia Keddari ◽  
Narimen Benaoum ◽  
Yasmina Mokhtaria Boufadi ◽  
Mansouria Belhocine ◽  
Ali Riazi
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Phenolic Compounds ◽  
Biological Activities ◽  
Antibacterial Properties ◽  
Ethanolic Extract ◽  
Diffusion Method ◽  
E Coli ◽  
Ammi Visnaga ◽  
Wide Range

Medicinal plants have been used for countries as cures for human diseases because they contain components of therapeutic value. Among these medi-cinal plants, Ammi visnage which have an immense reservoir of potential compounds attributed to the secondary metabolites which have the advan-tage of being of great diversity of chemical structure and have a very wide range of biological activities. The objectives of the present work were to stu-dy the antioxidant and antimicrobial activity of phenolic compounds ex-tracted from A. visnaga L. Its extraction is performed by two methods, etha-nol extraction and water extraction. The results showed that A. visnaga L.. ethanolic extract contains a mixture of phytochemical classes as polyphenol, flavonoids and revealed that this plant has high antioxidant activity (IC50 0.069 mg/ml). Regarding the antimicrobial activity results expressed by the diameter of the inhibition zones by diffusion method AWDT, the most signifi-cant inhibition was observed against to Staphylococcus aureus (12 mm) to the ethanol extract at concentration of 100mg / ml. Thus the aqueous ex-tract had a significant inhibitory activity against on the strains Staphylococ-cus aureus (8 mm), E. coli ATCC 10536 (8 mm) to a concentration of 100 mg / ml. The results for the antibacterial properties have shown that Gram-positive bacteria (Staphylococcus aureus, Listeria monocytogenes and M. luteus.) were more sensitive than gram-negative (Pseudomonas aeruginosa, E. coli ATCC 10536) against from the action of phenolic compounds of the Ammi visnaga ethanolic extract.

scholarly journals PRELIMINARY PHYTOCHEMICAL SCREENING AND IN VITRO ANTI-MICROBIAL ACTIVITY OF ETHANOLIC EXTRACT OF STEMS OF THE PLANT TAGETES ERECTA L. (FAMILY-ASTERACEAE)

2018 ◽  
Vol 10 (4) ◽  
pp. 64
Author(s):  
Jafar Ali ◽  
Dibyendu Shil
Keyword(s):  
Antimicrobial Activity ◽  
Microbial Activity ◽  
Ethanolic Extract ◽  
Diffusion Method ◽  
Tagetes Erecta ◽  
Phytochemical Analysis ◽  
Standard Drug ◽  
Phytochemical Constituents ◽  
Tagetes Erecta L

Objective: To estimate the anti-microbial activity of ethanolic extract of Tagetes erecta L. (family-Asteraceae) in association with phytochemical analysis.Methods: The ethanolic extract of the stems of TageteseErecta L. was prepared and investigate for phytochemical constituents using standard methods. Antimicrobial activity of ethanolic extract of has been carried out against one Gram positive bacteria-Staphylococcus aureus and one Gram negative bacteria-Escherichia coli, and the antifungal activity of the plant extract was evaluated on Candida albicans species. The testing was done by the disc diffusion method to visualize the zone of inhibition of ethanolic extract was compared with that of standard drug i.e., Ciprofloxacin for anti-bacterial activity and Terbinafine for anti-fungal activity. The MIC was determined by the microdilution method (Cup-plate method).Results: The present investigation shows the phytochemical analysis, antimicrobial activity of the ethanolic extract of the stems of Tagetes erecta L. Various phytochemical analysis confirmed the presence of alkaloids, saponin glycosides, flavonoids, phenol, proteins and amino acids, cardiac glycosides, steroids, tanin and phenolic compounds. The anti-microbial activity of the ethanolic extract of the plant showed significant results against all three of the test organisms in compare to the standard drugs.Conclusion: The present study concluded that ethanolic extract of-of the stems of Tagetes erecta L. Proved the existence of phytochemical constituents. The ethanolic extract of the plant was found to possess promising antimicrobial activity when compared with the standards and it can be useful for the development of the antibiotics.

scholarly journals PRELIMINARY PHYTOCHEMICAL SCREENING AND IN VITRO ANTI-MICROBIAL ACTIVITY OF ETHANOLIC EXTRACTS OF SEEDS OF PUNICA GRANATUM AGAINST STANDARD PATHOGENIC STRAINS

2018 ◽  
Vol 10 (4) ◽  
pp. 55
Author(s):  
Aditya Jyoti Bora ◽  
Biswajit Dash ◽  
Sujata Paul ◽  
Bikash Gupta
Keyword(s):  
Antimicrobial Activity ◽  
Microbial Activity ◽  
Sulfhydryl Group ◽  
Ethanolic Extract ◽  
Punica Granatum ◽  
Diffusion Method ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Anti Fungal

Objective: To estimate the anti-microbial activity of ethanolic extract of Punica granatum seeds (family-Punicaceae) in association with phytochemical analysis.Methods: The ethanolic extract of the seeds of Punica granatum was prepared and investigate for phytochemical constituents using standard methods. Antimicrobial activities of ethanolic extract were carried out against one Gram positive bacteria (Staphylococcus aureus) and one Gram negative bacteria (Escherichia coli). The anti-fungal activity of the plant extract was evaluated on Candida albicans. The testing was done by the disc diffusion method. A zone of inhibition of ethanolic extract was compared with that of standard Tetracycline for anti-bacterial activities and Fluconazole for anti-fungal activity.Results: The result of this study showed a good antibacterial effect of pomegranate juice against both Gram negative and Gram positive bacteria. The antimicrobial activity of the fruit may be due to its high content of polyphenols which causes phenolic toxicity by interfering with the sulfhydryl group of the bacterial proteins and high content of tannins which interferes with bacterial membrane stability.Conclusion: The present study concluded that ethanolic extract of the seeds of Punica granatum contains the high existence of phytochemicals. The ethanolic extract of the plant was found to possess promising antimicrobial activity when compared with the standards.

scholarly journals Antimicrobial Activity of Some Medicinal Plants from East and Central Part of Nepal

2014 ◽  
Vol 2 (1) ◽  
pp. 88-92 ◽  
Author(s):  
Bimala Subba ◽  
Prakash Basnet
Keyword(s):  
Antimicrobial Activity ◽  
Ethanolic Extract ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Proteus Vulgaris ◽  
Disc Diffusion Method ◽  
E Coli ◽  
Ethanolic Extracts ◽  
Inhibitory Activities

To evaluate the antimicrobial activities of extract of eleven plants were examined against four common bacterials. The ethanolic extracts of various plants such as  Cissus repens, Hedyotis scandens, Jatropha curcas, Morus alba, Inula cappa, Equisetum ramosissimum, Osyris wightiana, Alternantheria sessilis and Hibiscus lampas  investigated individually for antimicrobial activity by disc diffusion method. These were investigated against selected species of Staphylococcus aureus, Escherichia coli, Proteus vulgaris and Klebsiella pneumoniae to find the inhibitory activities of the microbes. The ethanolic extract of C. repens showed considerably high activity against P. vulgaris, E. coli and S. aureus than other extracts.DOI: http://dx.doi.org/10.3126/ijasbt.v2i1.9697Int J Appl Sci Biotechnol, Vol. 2(1): 88-92

scholarly journals STUDY OF THE BIOLOGICAL AND ANTI-MICROBIAL ACTIVITY OF AN ETHANOLIC EXTRACT-CALLIGONUM LEUCOCLADUM B.

2020 ◽  
Vol 5 (3) ◽  
Author(s):  
Sayan Zharylgassynov
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Elemental Composition ◽  
Dosage Form ◽  
Chemical Elements ◽  
Arid Zone ◽  
Antibacterial Properties ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
E Coli

The biological and antimicrobial activity of the white zhuzgun plant (Calligonum leucocladum), which grows in the arid zone of South Kazakhstan, was studied. Qualitative reactions revealed the presence in the dosage form of juzgun flavonoids, coumarins, alkaloids, tannins. The activity of a phytopreparation against Staphylococcus aureus, E. coli was established.During assessment of the antibacterial properties of the ethanol extract of Calligonum leucocladum B., was established that higher degrees of dilution determine a pronounce antimicrobial action in relation to the microflora under study. The elemental composition of Calligonum leucocladum was studied. In the dosage form of Calligonum leucocladum revealed the presence of 32 chemical elements.

scholarly journals ANTIMICROBIAL ACTIVITY OF ETHANOLIC EXTRACTS FROM LEAVES OF PISTACIA INTEGERRIMA (ANACARDIACEAE) STEW EX BRAND

2020 ◽  
pp. 90-92
Author(s):  
HEM RAJ VASHIST ◽  
SHIVANI DOGRA
Keyword(s):  
Antimicrobial Activity ◽  
Herbal Remedy ◽  
Ethanol Extract ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
Coagulase Negative Staphylococcus ◽  
Klebsiella Pneumonia ◽  
Zone Of Inhibition ◽  
Disk Diffusion Method ◽  
E Coli

Purpose: The ethanol extract of leaves of Pistacia integerrima (EEPI) was evaluated for antibacterial activity. Methods: The leaves of P. integerrima were collected from wild source and authenticated by Dr. Manoj Joshi, botanist environmental education expert, Una, HP, India. Antimicrobial activity was performed using the Mueller–Hinton (MH) agar using disk diffusion method. In the MH agar media 38 g after dissolving in 1000 ml of distilled water (pH 7.3±0.2), the extract was incorporated such that concentration per ml will be 66.67 μg, 133.33 and, 166.66. Results: The minimum inhibitory concentration recorded was 133.33 for Pseudomonas aeruginosa, Salmonella Typhi, and Klebsiella pneumonia, whereas 166.6 for Staphylococcus aureus, Coagulase-negative Staphylococcus, and Escherichia coli. The maximum zone of inhibition was found 19 mm and 18 mm for S. aureus and E. coli, respectively. S. aureus have been reported for skin pneumonia, heart valve, and bone infections whereas E. coli causes diarrhea and many other infections in children. Conclusion: After reporting the better zone of inhibition for these two bacteria, EEPI can be used to formulate better herbal remedy against them.

scholarly journals Phytochemical profiling, antioxidant potentiality and antibacterial activity of the ethanolic extracts of Rosenvingea sp. of Bay of Bengal

2021 ◽  
Vol 45 (1) ◽  
pp. 59-71
Author(s):  
Md Abdul Alim ◽  
Md Farzanoor Rahman ◽  
Tamim Ahsan ◽  
Toufiqul Islam ◽  
Md Morshedul Alam ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Bay Of Bengal ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Klebsiella Pneumonia ◽  
Agar Disc

The objective of the present study was to explore the phytochemical profiling, antioxidant and antimicrobial activity of 50% ethanolic extract of Rosenvingea sp. found in the Bay of Bengal of Bangladesh. Seven phytochemicals were tested from the ethanolic extract of Rosenvingea, where four phytochemicals, namely steroid, glycosides, alkaloids, and tannins were present. However, ethanol extract exhibited low antioxidant activity compared to standard ascorbic acid as measured by DPPH-Free radical scavenging assay. In Brine Shrimp lethality bioassay, 50% ethanolic extract showed an LC50 value of 10.88 mg/mL, whereas positive control (K2Cr2O7) showed LC50 59.97 µg/mL suggesting the less toxic property of the ethanolic extract. In-vitro antimicrobial activity of ethanolic extract of Rosenvingea sp. was investigated against gram-positive and gram-negative bacteria species (Staphylococcus, Bacillus, Pseudomonas, Salmonella, and Klebsiella) by agar disc diffusion method. The highest antibacterial activity was noticed against Klebsiella pneumonia, Pseudomonas aeruginosa, Staphylococcus hominis with a zone of inhibition of 5.00±1.00 mm, 3.66±0.57 mm, and 3.33±0.57 mm, respectively. This is the first study on Rosenvingea sp. from the Bay of Bengal, reporting its phytochemical, antioxidant, and antimicrobial potentiality. However, more study is required to elucidate its commercial viability in the food and medicine industries. J. Bangladesh Acad. Sci. 45(1); 59-71: June 2021

scholarly journals Comparative antimicrobial potential of Tribhuvana-Mishrana and its ingredients against clinical bacteria

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Vashisht Kiran ◽  
Thakur Vivek ◽  
Kumar Deepak ◽  
Sharma Khemchand
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Effect ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Single Drug ◽  
Microbial Infections ◽  
Agar Well Diffusion Method

There are many single drug and compound formulations prescribed for Jwara (~ fever) in Ayurvedic classics. Tribhuvanakirti Rasa (TKR), Godanti Bhasma (GB) and Sudarshana Ghana Vati (SGV) are widely used compound formulations that are separately indicated for the treatment of Jwara (~fever) in different dosage schedules. In Ayurvedic Formulary of India Tribhuvana-Mishrana (TM) has been chiefly indicated in treatment of Sarva Jwara (~ fever). Therefore, in this study comparative antimicrobial activity of TM and its individual ingredients viz. TKR, GB and SGV were evaluated against four fever-causing microbes such as gram-positive bacteria Staphylococcus aureus and the gram-negative bacteria Salmonella typhii, Escherichia coli and Pseudomonas aeruginosa. The aqueous extract of all the four samples (formulation as whole and the three individual ingredients separately) in a dose 125mcg/ml were tested for their antimicrobial activity against four different microbes by agar well diffusion method. The study shows that TM and its ingredients independently show significant antimicrobial activity. S. aureus was found to be most sensitive to SGV and moderately sensitive to TKR and TM while S. typhii was found to be most sensitive to TM as compared to others. TKR was found to be most effective against E.coli while SGV and TM both showed significant antimicrobial effect against E. coli. TM could provide an alternative to synthetic antibiotics against human microbial infections.

Bioenhancing Effect of Cow Urine Distillate and Pepper Extract on Antibacterial Activity of Azadirachta Indica Leaves

1970 ◽  
Vol 9 (2) ◽  
pp. 3212-3214
Author(s):  
Pramod Dhakal ◽  
Ankit a Achary ◽  
Vedamurthy Joshi
Keyword(s):  
Antibacterial Activity ◽  
Azadirachta Indica ◽  
Pathogenic Bacteria ◽  
Ethanol Extract ◽  
Watery Diarrhea ◽  
Diffusion Method ◽  
E Coli ◽  
Drug Molecules ◽  
Cow Urine

Bioenhancers are drug facilitator which do not show the typical drug activity but in combination to enhance the activity of other molecule in several way including increase the bioavailability of drug across the membrane, potentiating the drug molecules by conformational interaction, acting as receptor for drug molecules and making target cell more receptive to drugs and promote and increase the bioactivity or bioavailability or the uptake of drugs in combination therapy. The objective of the present study was to evaluate the antibacterial and activity of combination in Azadirachta indica extract with cow urine distillate and pepper extract against common pathogenic bacteria, a causative agent of watery diarrhea. It has been found that Indian indigenous cow urine and its distillate also possess bioenhancing ability. Bioenhancing role of cow urine distillate (CUD) and pepper extract was investigated on antibacterial activity of ethanol extract of Azadirachta indica. Antibacterial activity of ethanol extract neem alone and in combination with CUD and pepper extract were determined the ATCC strains against Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa and E-coli by cup plate diffusion method. Ethanol extract of neem has showed more effect on P. aeruginosa, E-coli than S. aureus and K. pneumonia with combination of CUD and pepper extract. CUD and pepper did not show any inhibition of test bacteria in low concentration. The antibacterial effect of combination of extract and CUD was higher than the inhibition caused by extract alone and is suggestive of the bioenhancing role of cow urine distillate and pepper. Moreover, inhibition of test bacteria was observed with less concentration of extract on combining with CUD

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