Potential Changes in Watermelon (Citrullus lannatus) Ploidy Treated By Colchicine

The purpose of this study was to determine the effect of colchicine on changes in ploidy watermelon. The research was conducted in Agroecotechnology laboratory Universitas Malikussaleh, Microtechnique laboratory Agronomy and Horticulture Departement, Bogor Agricultural University, and Beuringen, Murah Mulia subdistrict, North Aceh. This research used Completely Randomized Design (CRD) two factors. The first factor was watermelon seed soaking time in colchicines 0,02% ie.0, 24,36, and 48 hours. The second factor was the concentration of the colchicine solution on the sprout growth point i.e. 0, 0,1, and 0,2 %. The results showed that plants were given colchicine became to shorter and fewer number of leaves than plants without any treatment. Colchicine could increase the size of the stomata, but it did not change the shape of stomata. Plants that were given colchicine had the potential to ploidy multiplication.

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Response of Shallots of Batu Ijo Variety on Doses of N And K Fertilizers

Akta Agrosia ◽

10.31186/aa.24.1.19-24 ◽

2021 ◽

Vol 24 (1) ◽

pp. 19-24

Author(s):

Widodo Widodo ◽

Marlin Marlin ◽

Nancy B. Sitio

Keyword(s):

Plant Growth ◽

N Fertilizer ◽

Tuber Weight ◽

Plant Weight ◽

Randomized Design ◽

K Fertilizer ◽

Two Factors ◽

Number Of Leaves ◽

Completely Randomized Design ◽

Higher Doses

Shallots (Allium cepa var. Aggregatum L) have many varieties that can be grown in the highlands and lowlands and one of them is the Batu Ijo variety. Nutrients N and K play an important role for plant needs. This research was conducted from March to June 2019 in Medan Baru, Bengkulu City. This study used a completely randomized design with two factors. The first factor was the dose of N fertilizer and the second factor was the application of K fertilizer. The application of N fertilizer had a significant effect on the number of shallots at week 2. The Urea dose given decreases the number of spring onions. For the best treatment at plant height is the treatment of Urea 300 kg/ha and KCl 50 kg/ha. Plant growth from week 2 to 5 increased significantly. The application of K fertilizer has an effect on the number of shallots at week 2 and the number of tubers. The higher the plant age, the more the number of leaves produced, but at higher doses, the number of leaves and the number of tubers decreased. The best treatment for the number of leaves produced was Urea 300 kg/ha and without giving KCl. For the best treatment of fresh plant weight, namely Urea 300 kg/ha and KCl 150 kg/ha, the best treatment fresh tuber weight was Urea 300 kg/ha and KCl 150 kg/ha, for tuber diameter the best treatment was 200 kg/ha of urea and KCl 100 kg/ha and for the number of tubers of Urea 0 kg/ha and KCl 150 kg/ha.Keywords: shallots, N fertilizer, K fertilizer

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Growth Induction of Cavendish Buds (Musa Acuminata L.) on Difference Concentration of IBA and BA in Vitro

Asian Journal of Agriculture and Rural Development ◽

10.18488/journal.1005/2018.8.2/1005.2.178.187 ◽

2019 ◽

Vol 8 (2) ◽

pp. 178-187

Author(s):

Subandi M ◽

Arkhan Jannata ◽

Sofiya Hasani

Keyword(s):

Musa Acuminata ◽

Laboratory Research ◽

Independent Effect ◽

Randomized Design ◽

Two Factors ◽

Number Of Leaves ◽

Completely Randomized Design ◽

Range Test ◽

Growth Induction

The propagation of Cavendish (Musa acuminata L.) seedling conventionally relatively took a longer time, not uniformed in quality and possibly has a disease that is derived from unhealthy mother stock. This research aimed to find the effect of IBA and BA on difference concentrations on the growth of plantlet, also to determine the optimum concentration of IBA and BA on the growth of Cavendish bud plantlets in vitro. This research had been carried out started from April to July 2018 in Tissue Culture Laboratory, Research Institute of Horticultural Seed Development of Pasir Banteng, Sumedang, West Java. Indonesia. The method used in this research was Completely Randomized Design with two factors. The first factor was IBA (i0 = 0 mg L-1, i1= 0,75 mg L-1, dan i2= 1,5 mg L1), and the second factor was BA (b1= 1 mg L-1 b2= 3 mg L-1 b3= 5 mg L-1 b4= 7 mg L-1), replicated three times. Duncan Multiple Range Test (DMRT) was used as a further test. The result showed an interaction between IBA and BA on the parameters of shoot growth and the number of leaves at 6 WAC. IBA showed an independent effect on the number of buds. The addition of 1,5 mg L-1 IBA and 3 mg L-1 BA was optimum for the growth of Cavendish bud explants.

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The Influence Of Giving Fertilizer And Fertilizer (N) Fertilizer Time On Plant Growth And Production Sawi Pakcoy (Brassica rapa L.)

Nabatia ◽

10.21070/nabatia.v6i1.980 ◽

2018 ◽

Vol 6 (1) ◽

pp. 43

Author(s):

Abdul Wachid ◽

Achmad Sairi

Keyword(s):

N Fertilization ◽

Fertilizer N ◽

Randomized Design ◽

Affected Plant ◽

Significant Difference ◽

Two Factors ◽

Wet Weight ◽

Number Of Leaves ◽

Completely Randomized Design ◽

Goat Manure

The average pakcoy mustard production in Indonesia is still quite low at 20 tons / ha. This study aims to determine the effect of goat manure and nitrogen fertilizer (N) on the growth and production of mustard greens. This research was carried out in Plaosan village, Wonoayu sub-district, Sidoarjo regency, using factorial completely randomized design with 2 factors, factor 1: without goat manure (control) (P0), goat manure 100 grams / polybag (P1 ), 200 gram goat manure / polybag (P2). Factor 2: giving nitrogen at 7 hst (K1), giving nitrogen at 14 hst (K2), giving nitrogen at 21 hst (K3). Of the two factors, 9 treatment combinations were repeated and repeated 3 times so that 27 experimental units were obtained. The results showed that goat manure significantly affected plant height, number of leaves and wet weight. However, when giving nitrogen (N) there was no significant difference in each treatment, while there was interaction between goat manure and nitrogen (N) fertilization time on the wet weight of mustard pakcoy plants with the best treatment in K2P2 with an average of 231,8.

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APLIKASI KOMPOS TANDAN KOSONG KELAPA SAWIT DAN DOLOMIT PADA MEDIUM SUB SOIL INCEPTISOL TERHADAP BIBIT KELAPA SAWIT (Elaeis guineensis Jacq.) DI PEMBIBITAN UTAMA

JURNAL AGROTEKNOLOGI ◽

10.24014/ja.v8i2.3349 ◽

2018 ◽

Vol 8 (2) ◽

pp. 1 ◽

Cited By ~ 1

Author(s):

Al Ichsan Amri ◽

Armaini Armaini ◽

Mazmur Rahmat Amindo Purba

Keyword(s):

Analysis Of Variance ◽

Palm Oil ◽

Elaeis Guineensis ◽

Dry Weight ◽

Real Effect ◽

The Real ◽

Randomized Design ◽

Two Factors ◽

Number Of Leaves ◽

Completely Randomized Design

The purpose of this research is determining the effect of some treatment combinations and to determine the combination of best compost bunches empty palm oil and dolomite that could support growth seeds palm oil plantations in a main nursery. This research has been conducted in the experimental field, Agriculture Faculty, University of Riau from April to July 2015. The research used Completely Randomized Design Factorial that consisting of two factors. The first factor is giving compost bunches empty palm oil : (37,5, 75 and 112,5 g/polybag). The second factor is giving lime dolomite (CaMg (CO3)2) : (9, 18 and 30 g/polybag). Each combination made three replications and two plants. The data were statistically analyzed using analysis of variance and the means were tested with Duncan 5%. The research results show that combination of compost bunches empty palm oil and dolomite had show the real effect on the increase in diameter hump of seedlings , but the effect not real on the parameters height of seedlings,increase number of leaves, volume roots, ratio crown rootsand and dry weight of seedlings. Combination compost bunches empty palm oil 112,5 g/polybag and dolomite 18 g/polybag showed better results for the growth of palm oil seedlings.

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Effect of Urea and SP-36 on the Growth of Grafted Rubber Seedling (Hevea brasiliensis Muell.Arg.) Clone PB 260

AGRIFOR ◽

10.31293/af.v18i1.4112 ◽

2019 ◽

Vol 18 (1) ◽

pp. 109

Author(s):

Helilinawati Helilinawati ◽

Hery Sutejo ◽

Abdul Fatah

Keyword(s):

Plant Height ◽

Stem Diameter ◽

Seedling Height ◽

East Kalimantan ◽

Randomized Design ◽

Two Factors ◽

Number Of Leaves ◽

Completely Randomized Design ◽

Four Levels ◽

The Village

The aim of research to determine the effect of urea and SP-36 fertilizer as well as their interaction on the graftedrubber seedling growth, and to find appropriate doses of urea and SP-36 fertilizers for better growth of grafted rubber seedlings.The research was conducted from February 2015 until May 2015 in the village of Empas, Sub District of Melak, West Kutai Regency, East Kalimantan Province.The study design used was completely randomized design (CRD) in a factorial 4 x 4, and repeat 3 times, which comprises two factors research. The first factor was Urea (N), consisted of four levels, namely: no urea application or control (n0), 2,5g/polybag (n1), 5g/polybag (n2), and 7,5g/polybag (n3). The second factor was SP-36 fertilizer (P), consisting of 4 levels, namely: no SP-36 fertilizers application or control (p0), 2,5g/polybag (p1), 5g/polybag (p2), and 7,5g/polybag (p3).The results showed that the treatment of urea (N) affects highly significant on the plant height at ages of 2, 3 and 4 months after grafting, number of leaf at 4 months after grafting and stem diameter at 3 and 4 months after grafting. It affected significantly on the number of leaf at 3 months after grafting. But it did not affect significantly on the number of leaf at 2 months after grafting, and stem diameter at 2 months after grafting.SP-36 fertilizer treatments (P) affected very significantlyon the stem diameter at 3 and 4 months after grafting. It affected significantly on the plant height at 4 months after grafting. But it did not affect significantly on the seedling height at 2 and 3 months after grafting, number of leaves at 2, 3 and 4 months after grafting, and stem diameter at 2 months after grafting.The interaction treatment (NXP) affected significantly on the stem diameter at 4 months after grafting. But it did not affect significantly on the seedling height at 2, 3 and 4 months after grafting, number of leaves at 2, 3 and 4 months after grafting, and stem diameter at 2 and 3 months after grafting

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Pengaruh Pemberian Pupuk Kandang Kotoran Ayam dan Pupuk NPK Mutiara Yaramila Terhadap Pertumbuhan dan Hasil Tanaman Terung (Solanum Melongena L.) pada Tanah Berpasir

Anterior Jurnal ◽

10.33084/anterior.v14i2.179 ◽

2015 ◽

Vol 14 (2) ◽

pp. 147-153 ◽

Cited By ~ 2

Author(s):

Mohammad Hertos

Keyword(s):

Sandy Soil ◽

Solanum Melongena ◽

Chicken Manure ◽

Growth And Yield ◽

Randomized Design ◽

Two Factors ◽

Number Of Leaves ◽

Completely Randomized Design ◽

Experimental Treatments

The purpose of this research was to find Chicken Manure and NPK Mutiara Yaramila Fertilizer on the growth and yield eggplant (Solanum melongena L.) in sandy soil. The research was arranged in a Completely Randomized Design with two factors and three duplications. The first factor was to find chicken manure (K) on the three levels : 20 ton/ha (K1), 30 ton/ha (K2) and 40 ton/ha (K3), the second factor was NPK Mutiara Yaramila Fertilizer (Y) on the three level : 200 kg/ha (Y1), 300 kg/ha (Y2) and 400 kg/ha (Y3). The results show the experimental treatments of chicken manure and NPK Mutiara Yaramila Fertilizer was significantly on the parameters: plant high, number of leaves, number of productive branches, number of fruit and weight of fruit. The highest yield to parameters of plant high in old 2, 4 and 6 MST (25,30 cm, 36,37 cm and 60,83 cm) number of leaf in old 2, 4 and 6 MST (8,67 sheet, 11,00 sheet and 23,33 sheet), number of productive branches (8,67 branches), number of fruit (4,33 fruits) and weight of fruit (417,23 g), obtainable on the treatments to find chicken manure as big as 40 ton/ha and NPK Mutiara Yaramila Fertilizer as big as 300 kg/ha.

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Budi daya Terapung Tanaman Sawi Hijau dengan Perbedaan Dosis Pupuk NPK, Ukuran Polibag, dan Waktu Pemupukan

Jurnal Ilmu Pertanian Indonesia ◽

10.18343/jipi.26.1.136 ◽

2021 ◽

Vol 26 (1) ◽

pp. 136-142

Author(s):

Erna Siaga ◽

Benyamin Lakitan

Keyword(s):

Growth And Yield ◽

Riparian Wetland ◽

Floating Raft ◽

Yield Parameters ◽

Randomized Design ◽

Two Factors ◽

Number Of Leaves ◽

Completely Randomized Design ◽

Flooding Period ◽

Plot Design

Cultivation of mustards green on floating raft is one of alternative cultivations which is applied by traditional farmers during flooding period in Riparian Wetland. The cultivation will increase Cropping Index and farmer’s income. The objective of this research is to evaluate growth and yield of mustards green on floating cultivation with the application of different NPK doses, polybag sizes, and fertilization times to find cultivation of mustards green which effective and efficient. This research was conducted on October 2016–February 2017 in Jakabaring Facility (104°46’44” E; 3°01’35” S) and Kimia Hasil Pertanian (LKHP) Laboratory, Fakultas Pertanian, Universitas Sriwijaya, Palembang. This research consisted of two steps. The first experiment used a completely randomized design with four doses of NPK (16:16:16) namely 0 (control), 5, 10, and 15 g plant-1. The second experiment used a Split Plot Design consisted of two factors namely polybag size (A1 and A2) and fertilization time (F1, F2, and F3). The result of the first experiment showed that utilization three doses of NPK significantly increased shoot length, number of leaves, and fresh weight compared control. The second experiment showed that polybag size and fertilization time did not significantly affect growth and yield parameters. Utilization of 5 g plant-1 of NPK on 1 week after transplanting (WAT) which used polybag size of 30 x 30 cm on growing media consisted of soil, manure, and rice husk (1:1:1/v:v:v) produced an effective and efficient cultivation of mustards green. Keywords: fertilizing, floating cultivation, green mustard, NPK

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PENGARUH KONSENTRASI DAN LAMA PERENDAMAN STEK LADA (Piper nigrum) DALAM LARUTAN ZAT PENGATUR TUMBUH (AUKSIN)

JURNAL ILMIAH AGRINECA ◽

10.36728/afp.v20i2.1086 ◽

2020 ◽

Vol 20 (2) ◽

pp. 158-169

Author(s):

Teguh Supriyadi ◽

Tyas Soemarah K.D ◽

Endang Suprapti ◽

Agus Budiyono

Keyword(s):

Growth Regulators ◽

Dry Weight ◽

Piper Nigrum ◽

Fresh Weight ◽

Soaking Time ◽

Root Dry Weight ◽

Randomized Design ◽

Number Of Leaves ◽

Completely Randomized Design ◽

Number Of Shoots

The research entitled "The Effect of Concentration and Soaking Time of Pepper Cutters (Piper nigrum) in a Solution of Growth Regulatory Substances (auxin)" was carried out. Research was held on April – July 2020 in Green House of Agriculture Faculty, Tunas Pembangunan University, Jl. Balekambang Lor No. 1 Manahan, Surakarta. This study used a factorial experimental method based on a completely randomized design (CRD) consisting of 2 factors as treatment and 3 replications. The first factor, the concentration of growth regulators (auxin) (K), is divided into 3 levels, namely: 100 ppm (K1), 200 ppm (K2) and 300 ppm (K3); while the second factor, immersion time (P), also consists of 4 levels, namely: 2 hours (P1), 2.5 hours (P2), 3 hours (P3) and 3.5 hours (P4). The parameters observed in this study amounted to 9, with details as follows: Number of shoots, number of leaves, length of shoots, fresh and dry weight of shoots, number and length of roots, fresh weight and root dry weight. Based on the results of research on pepper cuttings due to the concentration of growth regulators (auxin) and the duration of immersion, the results showed that: Soaking time had a significant effect on the observed parameters, namely plant length in P2 treatment (2.5 hours). And it did not affect the parameters of the number of shoots, number of leaves, fresh and dry weight of shoots, number and length of roots, fresh weight and root dry weight.

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Pengaruh Konsentrasi Zat Pengatur Tumbuh Naphthalene Acetic Acid dan Benzil Amino Purin terhadap Pertumbuhan Protokorm Anggrek Dendrobium spectabile pada Kultur In Vitro

Paspalum: Jurnal Ilmiah Pertanian ◽

10.35138/paspalum.v7i1.107 ◽

2019 ◽

Vol 7 (1) ◽

pp. 16

Author(s):

Ujang Siron ◽

Noertjahyani Noertjahyani ◽

Yana Taryana ◽

Romiyadi Romiyadi

Keyword(s):

Research Method ◽

Dry Weight ◽

Naphthalene Acetic Acid ◽

Ms Medium ◽

Randomized Design ◽

Two Factors ◽

Number Of Leaves ◽

Completely Randomized Design ◽

Medium Culture

The aim of the article is to study the interaction effect between NAA and BAP concentration on protocorm growth and to know the proper concentration for growth of Dendrobium spectabile orchid protocorm. This research method using an experimental method which is conducted in the Laboratory of Agriculture Faculty of Winaya Mukti University, Tanjungsari Subdistrict, Sumedang District. The experiment was conducted from June 2017 until September 2017. The experiment used was a Completely Randomized Design (CRD) with the factorial pattern, consisting of two factors and repeated twice. the first factor was the effect of NAA concentration which consisted of five levels, namely without NAA, 0.5 mg kg-1, 1.0 mg kg-1, 1.5 mg kg-1, and 2.0 mg kg-1. The second factor is the BAP concentration which consists of five levels, namel without BAP, 1.0 mg kg-1, 2.0 mg kg-1, 3.0 mg kg-1, and 4.0 mg kg-1. Eksplant is protocorm from orchid D. spectabile which is grown on MS medium (Murashig and Skoog) half recipe as base medium accompanied by each treatment for 12 weeks. The experimental results show that there is an interaction between the effect of NAA and BAP concentration on the number of leaves only. Without NAA or 1.5 mg kg-1 NAA concentration with BAP 2.0 mg kg-1 gives more leaves. Independent of NAA or BAP concentrations did not affect the number of buds, number of roots, root length, fresh and dry weight of plantlets, and also growth ability of plantlets. BAP concentration only affect plant height, and the highest plantlet height is found without add of BAP in medium culture

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Artificial Autotetraploidy Induction Possibility of Two Iranian Endemic Mint (Mentha mozaffarianii) Ecotypes

Notulae Scientia Biologicae ◽

10.15835/nsb629129 ◽

2014 ◽

Vol 6 (2) ◽

pp. 185-191 ◽

Cited By ~ 4

Author(s):

Askar GHANI ◽

Seyyed Hossein NEAMATI ◽

Majid AZIZI ◽

Mohammad Jamal SAHARKHIZ ◽

Mohammad FARSI

Keyword(s):

Apical Meristem ◽

Colchicine Treatment ◽

Factorial Experiment ◽

The Other ◽

Chromosome Counts ◽

Soaking Time ◽

The Third ◽

Randomized Design ◽

Two Factors ◽

Completely Randomized Design

The present study was conducted to polyploidy possibility induction of two Iranian endemic mint (Mentha mozaffarianii) ecotypes. For this purpose, three experiments were done. The first experiment was factorial, based on completely randomized design with three factors and three replications that rhizomes were used for treatment. The first factor including different colchicine concentrations (0, 0.025, 0.05, 0.1 and 0.2% that 0 as control). The second factor including two Mentha ecotypes (Ecotype A: Kamarej region and Ecotype B: Pirmohlat region) and the third factors consist of two soaking time (6 h and 12 h). In second experiment, apical meristem was treated. The factorial experiment based on randomized completely design with two factors and five replications. The factors including different colchicine concentrations (0, 0.035, 0.07, 0.15, 0.3 and 0.7%) and two ecotypes. In the third experiment, colchicine as combined with irrigation was applied. For this purpose, a factorial experiment in randomized complete design with two factors (colchicine concentrations: 0, 0.025, 0.012, 0.006% and two mint ecotypes) and three replications was conducted. At the end of all experiments, survival rate and tetraploidy percentage (by morphological change, stomata traits, flow cytometry and chromosome counts) were measured. The results showed that different treatment had significant effects on survival percent on all experiments and increasing of colchicine concentration caused decreasing plants survival. On the other hand, tetraploidy changes only in the first experiments were observed. Only in 0.025% colchicine treatment with 6 h soaking time on ecotype A, 12.66% tetraploidy was identified. Totally, it is seems that Mentha mozaffarianiihardly response to colchicine for tetraploidy induction.

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