scholarly journals Artificial Autotetraploidy Induction Possibility of Two Iranian Endemic Mint (Mentha mozaffarianii) Ecotypes

2014 ◽  
Vol 6 (2) ◽  
pp. 185-191 ◽  
Author(s):  
Askar GHANI ◽  
Seyyed Hossein NEAMATI ◽  
Majid AZIZI ◽  
Mohammad Jamal SAHARKHIZ ◽  
Mohammad FARSI
Keyword(s):  
Apical Meristem ◽  
Colchicine Treatment ◽  
Factorial Experiment ◽  
The Other ◽  
Chromosome Counts ◽  
Soaking Time ◽  
The Third ◽  
Randomized Design ◽  
Two Factors ◽  
Completely Randomized Design

The present study was conducted to polyploidy possibility induction of two Iranian endemic mint (Mentha mozaffarianii) ecotypes. For this purpose, three experiments were done. The first experiment was factorial, based on completely randomized design with three factors and three replications that rhizomes were used for treatment. The first factor including different colchicine concentrations (0, 0.025, 0.05, 0.1 and 0.2% that 0 as control). The second factor including two Mentha ecotypes (Ecotype A: Kamarej region and Ecotype B: Pirmohlat region) and the third factors consist of two soaking time (6 h and 12 h). In second experiment, apical meristem was treated. The factorial experiment based on randomized completely design with two factors and five replications. The factors including different colchicine concentrations (0, 0.035, 0.07, 0.15, 0.3 and 0.7%) and two ecotypes. In the third experiment, colchicine as combined with irrigation was applied. For this purpose, a factorial experiment in randomized complete design with two factors (colchicine concentrations: 0, 0.025, 0.012, 0.006% and two mint ecotypes) and three replications was conducted. At the end of all experiments, survival rate and tetraploidy percentage (by morphological change, stomata traits, flow cytometry and chromosome counts) were measured. The results showed that different treatment had significant effects on survival percent on all experiments and increasing of colchicine concentration caused decreasing plants survival. On the other hand, tetraploidy changes only in the first experiments were observed. Only in 0.025% colchicine treatment with 6 h soaking time on ecotype A, 12.66% tetraploidy was identified. Totally, it is seems that Mentha mozaffarianiihardly response to colchicine for tetraploidy induction.

scholarly journals Analisis Efektifitas Tepung Jamur sebagai Larvasida Aedes aegypti

2012 ◽  
Vol 14 (1) ◽  
pp. 126
Author(s):  
Yekki Yasmin ◽  
Lenni Fitri ◽  
Betty Mauliya Bustam
Keyword(s):  
Aedes Aegypti ◽  
Beauveria Bassiana ◽  
Storage Time ◽  
Natural Sciences ◽  
The Other ◽  
Biology Department ◽  
Randomized Design ◽  
Two Factors ◽  
Result Show ◽  
Completely Randomized Design

This research is about effectivity analysis of two kinds of fungi i.e. Beauveria bassiana and Metharrizium anisopliae as Aedes aegyptilarvacide, it has been conducted at laboratories Zoology in Biology Department, Mathematics and Natural Sciences Faculty, Syiah KualaUniversity. Aims of this research were estimating maximum time storage of fungi powder as effective larvacide and the number of bacteriacolonies were found in water used for experiment. This research was used Completely Randomized Design (CRD). The experiments consistof two factors i.e kind and saving time of fungi. The result show that the longer the storage time of Beauveria bassiana the more ineffectiveit was as larvacide and the least fungi colonies were found. Metharrizium anisopliae on the other hand, the longer it was kept, the moreeffective it was as larvacide and the more fungi colonies were found.

scholarly journals Mutasi Induksi Dendrobium sylvanum var. flava Menggunakan Kolkisin secara In Vitro

2018 ◽  
Vol 9 (1) ◽  
pp. 54-62
Author(s):  
Musalamah , ◽  
Ni Made Armini Wiendi ◽  
Sri Rianawati
Keyword(s):  
Analysis Of Variance ◽  
Colchicine Treatment ◽  
Chloroplast Number ◽  
The Third ◽  
Proliferation Medium ◽  
Randomized Design ◽  
Completely Randomized Design

ABSTRACTIn Vitro mutation using colchicine on 2 month of self-pollinated protocorm like bodies of Dendrobium sylvanum var. flava was conducted to determine the effects of concentration and immersion duration in colchicine on proliferation of PLBs, and to identify of ploidy variants based on stomatal variable. Research was arranged using factorial completely randomized design with three factors in three replications. The first factor was concentration of colchicine, consisted of five concentrations (0.02; 0.04, 0.06; 0.08; dan 0.1%). The second factor was duration of immersion in the colchicine, consisted of four durations (1; 24; 48; 72 hours). The third factor was proliferation medium consisted of two concentrations of BAP (1; 0.5 mg L-1). Analysis of variance showed the significant effect of colchicine treatment on percentage of survived explants. LD50 in media 1 mg L-1 BAP was obtained at a colchicine concentration of 0.069% with duration immersion of 58.19 hours. On Media 0.5 mg L-1 BAP, LD50 was obtained at colchicine concentration of 0.054% with duration immersion of 47.63 hours. Percentage of solid polyploid mutant of Dendrobium sylvanum var. flava can not be determined on MV2 generation because the stomata leaf showed chimeras based on the chloroplast number in cell guard and stomata size.Keywords: colchicines, Dendrobium sylvanum, mutation, number of chloroplast stomatal density.ABSTRAKMutasi dengan kolkisin pada PLBs hasil selfing Dendrobium sylvanum var. flava umur 2 bulan dilakukan secara In Vitro dengan tujuan mempelajari pengaruh konsentrasi kolkisin, durasi perendaman dalam kolkisin, media proliferasi terhadap pertumbuhan PLBs Dendrobium sylvanum var. flava serta mengidentifikasi variasi ploidi berdasarkan variabel stomata. Penelitian disusun dalam Rancangan Acak Lengkap Faktorial 3 Faktor dengan 3 ulangan. Faktor pertama ialah konsentrasi kolkisin yang terdiri atas 5 taraf (0.02; 0.04, 0.06; 0.08; dan 0.1%). Faktor kedua ialah durasi perendaman yang terdiri atas 4 taraf (1; 24; 48; 72 jam). Faktor ketiga ialah media proliferasi media V&W yang ditambah BAP terdiri atas 2 taraf (1; 0.5 mg L-1). Hasil analisis ragam menunjukkan pengaruh kolkisin yang nyata terhadap variabel persentase hidup. LD50 pada media 1 mg L-1 BAP diperoleh pada konsentrasi 0.069 % dengan durasi perendaman 58.19 jam. Pada media 0.5 mg L-1 BAP, LD50 diperoleh pada konsentrasi 0.054 % dengan durasi perendaman 47.63 jam. Persentase mutan poliploid pada MV2 Dendrobium sylvanum var. flava ini belum dapat ditentukan karena stomata daunnya masih kimera berdasarkan karakter jumlah kloroplas sel penjaga dan ukuran stomata.Kata kunci: Dendrobium sylvanum, jumlah kloroplas, kerapatan stomata, kolkisin, mutasi.

scholarly journals Potential Changes in Watermelon (Citrullus lannatus) Ploidy Treated By Colchicine

2018 ◽  
Vol 1 (1) ◽  
pp. 10
Author(s):  
Rd. Selvy Handayani ◽  
Muhamad Yusuf ◽  
Ajmir Akmal
Keyword(s):  
Soaking Time ◽  
Growth Point ◽  
Seed Soaking ◽  
Watermelon Seed ◽  
Randomized Design ◽  
Two Factors ◽  
Colchicine Solution ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Sprout Growth

The purpose of this study was to determine the effect of colchicine on changes in ploidy watermelon. The research was conducted in Agroecotechnology laboratory Universitas Malikussaleh, Microtechnique laboratory Agronomy and Horticulture Departement, Bogor Agricultural University, and Beuringen, Murah Mulia subdistrict, North Aceh. This research used Completely Randomized Design (CRD) two factors. The first factor was watermelon seed soaking time in colchicines 0,02% ie.0, 24,36, and 48 hours. The second factor was the concentration of the colchicine solution on the sprout growth point i.e. 0, 0,1, and 0,2 %. The results showed that plants were given colchicine became to shorter and fewer number of leaves than plants without any treatment. Colchicine could increase the size of the stomata, but it did not change the shape of stomata. Plants that were given colchicine had the potential to ploidy multiplication.

scholarly journals Analisis Lemak Susu Edamame dari Olahan Biji Edamame

2019 ◽  
Vol 2 (1) ◽  
Author(s):  
Nufan Muwafiq Sukiran ◽  
Hari Santoso ◽  
Ahmad Syauqi
Keyword(s):  
Fat Content ◽  
The Other ◽  
Soy Milk ◽  
Average Value ◽  
The Third ◽  
Randomized Design ◽  
Completely Randomized Design

Edamame milk (Glycin max. L var. edamame) is one product with the advantages; easily produced and highly nutritious. This study aims was to determine the fat content of edamame milk prepared by edamame bean. The method used in this study is the experiment using a completely randomized design with 4 treatments and 5 replications. Fat analysis was done using the Gerber method. The fat levels of edamame milk at the first treatment, the average value of Edamame milk was 5.74% , the second 5.28%, the third 4.56% and the last treatment or the the fourth is fat at 2.0%. The results are compared with the other fat content of the other varieties, then it can be compared the fat content of Edamame varietes lower than the variants of Malika and Grobogan which are variants of Edamame fat content which is 5.74%, Malika fat content was 13.2%, and the Grobogan variance averaged 12.83%. The results of the analysis show that there are significant differences between treatments. For the average edamame fat content that is good is equal to 2.02% and the results close to the standard of soy milk that is equal to 2.0%.Keywords: fat content, edamame milk, Gerber method.ABSTRAKSusu edamame (Glycin max. L var. edamame) merupakan salah satu produk dengan kelebihan; mudah diproduksi dan bergizi tinggi. Penelitian ini bertujuan adalah menentukan kadar lemak susu edamame olahan biji Edamame. Metode yang digunakan dalam penelitian ini yaitu eksperimen dengan Rancangan Acak Lengkap dengan 4 perlakuan dan 5 kali ulangan. Analisis lemak dilakukan menggunakan metode Gerber. Hasil penelitian kadar lemak susu edamame pada perlakuan pertama, nilai rata-rata ulangan lemak susu edamame yaitu sebesar 5,74%, kedua 5,28% ketiga 4,56% dan keempat 2,02% Jika hasilnya dibandingkan dengan kadar lemak varietes lainnya maka bisa di bandingkan kadar lemak varietes edamame lebih rendah dari varietes malika dan grobogan yang mana varietes edamame kadar lemaknya yaitu sebesar 5,74%. Malika kadar lemaknya sebesar 13,2%, dan varietes Grobogan rata-ratanya sebesar 12,83%. Hasil analisis menunjukkan bahwa terdapat perbedaan yang signifikan diantara perlakuan. Rata-rata kadar lemak edamame yang baik yaitu sebesar 2,02%  dan hasilnya mendekati standar susu kedelai sebesar 2,0%Kata kunci: kadar lemak, susu edamame, metode Gerber.

scholarly journals UJI BEBERAPA JENIS DAN DOSIS CENDAWAN MIKORIZA ARBUSKULA SERTA ADOPSI METODA SRI ( The System of Rice Intensification) TERHADAP PERTUMBUHAN DAN PRODUKSI PADI GOGO PADA ULTISOL

Jurnal Solum ◽  
2009 ◽  
Vol 6 (2) ◽  
pp. 74
Author(s):  
Yusnaweti Yusnaweti ◽  
Sevindrajuta Sevindrajuta
Keyword(s):  
Arbuscular Mycorrhizae ◽  
Upland Rice ◽  
Application Rate ◽  
Factorial Experiment ◽  
System Of Rice Intensification ◽  
Gigaspora Rosea ◽  
Randomized Design ◽  
Two Factors ◽  
Rice Intensification ◽  
Completely Randomized Design

An experiment about “Effects of Arbuscular Mychorhizae Fungi and Adoption of SRI Method on the Growth and Production of Upland Rice on Ultisol” was conducted in glasshouse and soil laboratory of Agriculture Faculty, University of Andalas, Padang. The objective was to obtain the best species and optimum rate of Arbuscular Mycorrhizaze Fungi (AMF) for increasing growth and productivity of upland rice in Ultisol. This was designed in a Factorial Experiment which was allocated in Completely Randomized Design, with two factors and four replications. The first factor was species of AMF (Glomus manihotis, Glomus fasiculatum, and Gigaspora rosea), and the second factor was the application rate of the AMF (0, 5, and 10 g/pot). Variety of upland rice being used was Danau Gaung. The results of the experiment indicated that AMF from species Glomus fasiculatum with 5 g/pot or planting hole showed the best growth and highest production of upland rice in Ultisol.Key words: Arbuscular Mycorrhizae Fungi (AMF), upland rice, Ultisol

scholarly journals Analisis Kadar Asam Asetat Dalam Media Limbah Fermentasi Biji Kakao Akibat Penambahan Konsentrasi Acetobacter Aceti Dan Waktu Inkubasi

2017 ◽  
Vol 23 (1) ◽  
pp. 67-72
Author(s):  
Wiwik Kusmawati
Keyword(s):  
Acetic Acid ◽  
Incubation Time ◽  
Acid Content ◽  
The Other ◽  
Test Results ◽  
Acetobacter Aceti ◽  
Cocoa Beans ◽  
Randomized Design ◽  
Two Factors ◽  
Completely Randomized Design

This study aims to determine the effect of Acetobacteraceti concentration and incubation time on levels of acetic acid in the vinegar fermentation of cocoa beans waste media. This study uses a completely randomized design (CRD) arranged as factorial, consisting of two factors and repeated three times. The frst factor is the concentration of Acetobacteraceti which consists of three levels, namely Acetobacteraceti starter concentration of 10% (A1), 13% (A2) and 16% (A3). While the second factor is the time of incubation for 6 days (W1), 8 days (W2) and 10 days (W3). Results of analysis of variance showed that the concentration of Acetobacteraceti and incubation time affect the concentration of acetic acid in the vinegar fermentation of cocoa beans waste media. While Duncan’s test results showed the treatment inoculation 13% starter Acetobacteraceti with 6 days of incubation time signifcantly different from the other treatments with maximum acetic acid content is 4,02%. So that these results meet the standards of vinegar.

scholarly journals Effects of Various Irrigation and Fertilizer on Water Efficiency and Tomato Yield (Solanum lycopersicum) in Alfisols

2021 ◽  
Vol 18 (2) ◽  
pp. 152
Author(s):  
Rahayu Rahayu ◽  
Aktavia Herawati ◽  
Aktavia Herawati ◽  
Nur Faizaturrohmah ◽  
Nur Faizaturrohmah
Keyword(s):  
Water Supply ◽  
Solanum Lycopersicum ◽  
Drip Irrigation ◽  
The Other ◽  
Water Efficiency ◽  
Plant Yield ◽  
Tomato Yield ◽  
Randomized Design ◽  
Two Factors ◽  
Completely Randomized Design

<p>Investing in irrigation is very important and strategic in the context of water supply for agriculture. Therefore, this study aims to identify the most efficient irrigation technique and type of fertilizer to maximize the yield of tomatoes (Solanum lycopersicum) in Alfisols. This study was a greenhouse pot experiment with a factorial completely randomized design with two factors consisting of four irrigation techniques (pottery, drip, 75% ETc manual, and 100% ETc manual) and types of fertilizers (without, organic, inorganic, mixed fertilizer). The results showed that pottery irrigation had the highest Nitrogen content in the soil and saved up to 50% water compared to 100% ETc. On the other hand, drip irrigation uses water of 75% ETc with similar plant yield results. The pottery irrigation was the most efficient irrigation method for growing tomato than 75% ETc manual irrigation, and 100% ETc manual irrigation with mixed fertilizers (I4P3) was the highest on tomato yield.</p>

scholarly journals PENELITIAN PERUBAHAN WARNA DAN ETILEN SELAMA PEMERAMAN DAUN TEMBAKAU KULTIVAR MADURA SERTA PENGARUHNYA TERHADAP MUTU TEMBAKAU RAJANGAN

2020 ◽  
Vol 7 (3) ◽  
pp. 93
Author(s):  
JOKO PURDIYANTO
Keyword(s):  
Carboxylic Acid ◽  
Nicotiana Tabacum ◽  
Colour Change ◽  
The Other ◽  
Tobacco Leaves ◽  
Ripening Process ◽  
The Third ◽  
Randomized Design ◽  
Completely Randomized Design

<p><strong>Colour alternation and et/tylene changes during ripening tobacco leaves cultivar Madura and its effect to the quality of slice Madura tobacco</strong></p><p>The colour change and elhylene production in the leaves of Madura tobacco (Nicotiana tabacum) during ripening was studied at "Labora¬ torium Biokimia Pusat Antar Universitas Pangan dan Gizi Universitas Gadjahmada Yogyakata" and "Laboratorium Inti dan Atom Jurusan I'isika, FMIPA Universitas Gadjahmada Yogyakata". The change of colour was measured with Chrommameter CR-200. while the production of ethylcne, ACC (1-aminocyclopropanc-l-carboxylic acid) and MACC (malonyl-ACC) were measured with Photoaccuslic Spectroscopy, from the sample of 100 kg tobacco leaves cv. Jepon grown from April to September 1995 in Plakpak, Pcgantenan, Pamekasan, East Java. The experiment was designed as a completely randomized design in three replicates. The leaves samples were taken randomly from the mature middle leaves Results showed that endogenous ethylcne production al the irst day al a level of 169 ppb. was able to initiate ripening. Conversion percentage of ACC to ethylcne for 8 days ripening increased from 7.7 up lo 61.4 %. F tests least signiicantly different (1%). showed that yellow intensity signiicantly increased on the second day. This indicated thai ripening process was stated on the second day. On the other hand green intensity significantly decreased at the ifth day, thus no chlorophyl degradation. Brightness was signiicantly observed on Ihe third day of ripening, meaning that ripening stated on the third day. Based on the three criteria, the best ripening period was from the third lo the ilth day. This implies that the farmers will have three day periods of ripening which can be arranged to overcome the shotage of man power for slicing the leaves.</p>

scholarly journals PENGARUH LAMA PERENDAMAN DAN KONSENTRASI ASAP CAIR TERHADAP PENILAIAN ORGANOLEPTIK DAN KIMIA POKEA (Batissa violacea var. celebensis, von Martens 1897) ASAP

2019 ◽  
Vol 2 (1) ◽  
pp. 110
Author(s):  
Prilya Zhatrawati Mentang ◽  
Moh Nuh Ibrahim ◽  
Kobajashi Togo Isamu
Keyword(s):  
Moisture Content ◽  
Protein Content ◽  
Water Content ◽  
Soaking Time ◽  
Liquid Smoke ◽  
Randomized Design ◽  
Two Factors ◽  
The Mean ◽  
Completely Randomized Design

AbstrakPenelitian ini bertujuan Untuk mengetahui pengaruh lama perendaman asap cair, konsentrasi asap cair interaksi antara lama perendaman dan konsentrasi asap cair terhadap mutu pokea asap.Penelitian ini menggunakan Rancangan Acak Lengkap Faktorial (RALF) yang terdiri dari dua faktor. Faktor pertama yaitu waktu perendaman (5, 10, 15 menit), faktor kedua yaitu konsentrasi asap cair (2%, 4%, 6%) dengan tiga kali ulangan. Pokea asapterhadap interaksi lama perendaman dan konsentrasi asap cair terhadap organoleptikrupa, bau, tekstur, rasa, kadar air dan kadar protein berpengaruh sangat nyata. Kadar benzo(a)pyrene pada T3N1 sebesar 2,47 mg/kg, T3N2 sebesar            5,46 mg/kg dan T3N3 sebesar 3,84 mg/kg. Rerata organoleptik rupa diperoleh nilai tertinggi pada T2N2 sebesar 4 (suka), bau pada perlakuan T2N3 sebesar 4 (suka), rasa pada perlakuan T2N3 sebesar 3 (agak suka), tekstur pada perlakuan T2N3 sebesar           4 (suka), kadar air pada perlakuan T3N1 sebesar 9,91% dan kadar protein pada perlakuan T2N3 sebesar 45,69%. Kata kunci: Pokea, asap cair, kadar air, kadar protein, organoleptik  AbstractThe aims of this study was to determine the effect of liquid smoke soaking time, liquid smoke concentration and interaction between soaking time and liquid smoke concentration on the quality of smoke pokea. This research used a Factorial Completely Randomized Design (FCRD) consisting of two factors. The first factor is soaking time (5, 10, 15 minutes), the second factor is the concentration of liquid smoke (2%, 4%, 6%) with three replications.Smokedpokea for interaction of soaking time and liquid smoke concentration on organoleptic appearance, odor, texture, taste, water content and protein content had a very significant effect. The level of benzo(a)pyrene on T3N1 was 2.47 mg/kg, T3N2 was 5.46 mg/kg and T3N3 was 3.84 mg/kg. The mean organoleptic obtained the highest value on T2N2 of 4 (likes), odor at T2N3 of 4 (likes), taste on T2N3 of 3 (rather like), texture on T2N3 of 4 (likes), water content on T3N1 was 9.91% and protein content in T2N3 was 45.69%.Key words: Pokea clam, liquid smoke, moisture content, protein content, organoleptic

Pengaruh Pemberian Moringa oleifera Terhadap Kuantitas dan Kualitas Semen Sapi Peranakan Ongole

2020 ◽  
Vol 7 (2) ◽  
pp. 119
Author(s):  
Lukman Affandhy ◽  
Muchamad Luthfi ◽  
Dian Ratnawati ◽  
Frediansyah Firdaus
Keyword(s):  
Data Analysis ◽  
Analysis Of Variance ◽  
Research Method ◽  
Moringa Oleifera ◽  
The Third ◽  
Randomized Design ◽  
Leaf Powder ◽  
Completely Randomized Design ◽  
Second Period

ABSTRAK  Penelitian bertujuan untuk mengetahui pengaruh pemberian bubuk daun Moringa oleifera (MO) terhadap kuantitas dan kualitas semen sapi peranakan ongole (PO). Metode penelitian menggunakan percobaan lapang dengan rancangan acak lengkap dengan tiga perlakuan pemberian MO sebesar 0 kg/ekor/hari (P1); 0,05 kg/ekor/hari (P2) dan 0,1 kg/ekor/hari (P3). Analsis data menggunakan one way analysis of variance. Masing-masing perlakuan terdiri atas empat ekor dengan tiga periode pengamatan sebagai ulangan, yaitu periode pertama dan ketiga tanpa MO, dan periode kedua diberikan MO pada P2 dan P3. Hasil penelitian menunjukan bahwa volume dan pH semen perlakuan P1, P2 dan P3 tidak berbeda semua periode pengamatan. Motilitas massa spermatozoa P2 dan P3 periode kedua dan ketiga menunjukan nilai +++ berbeda nyata (P<0,05) dibandingkan dengan P1 (++); motilitas individu  perlakuan P1 menunjukkan nilai terendah 60,56±2,94 % (P<0,05) dibandingkan P2 dan P3 pada periode kedua dan ketiga (75,50±2,29 dan 72,50±2,34%). Konsentrasi spermatozoa P2 dan P3 periode kedua dan ketiga menunjukkan rata-rata  >1.350 juta/ml berbeda nyata (P<0,05) daripada P1 (876±152juta/ml), sedangkan total spermatozoa motil menunjukkan nilai tertinggi 5.647±829 juta/ml pada P3 periode kedua.Viabilitas spermatozoa P2 dan P3 menunjukkan nilai >88-89 % beda nyata (P<0,05) daripada P1 (<84 %) pada periode kedua dan ketiga, sedangkan nilai abnormalitas spermatozoa yang terbaik adalah P2 sebesar 4,30% pada periode kedua dan P3 sebesar 5,33% pada periode ketiga. Disimpulkan pemberian bubuk daun MO dengan dosis 0,1 kg/ekor/hari dapat memperbaiki kuantitas dan kualitas semen (viabilitas dan total spermatozoa motil) sapi pejantan PO.Kata kunci:  kuantitas dan kualitas semen, Moringa oleifera, sapi pejantan ABSTRACTThe research aimed to determine the effect of Moringa oleifera (MO) leaf powder on the quantity and quality of semen of ongole crossbreed. The research method used was a field experiment with a completely randomized design with three treatments giving MO of 0 kg/head/day (P1); 0.05 kg/head/day (P2) and 0.1 kg/head/day (P3). Each treatment consisted of four heads with three periods of observation as replications, namely the first and third periods without MO, and the second period was given MO on P2 and P3. Data analysis used a one-way analysis of variance. The results showed that the volume and pH of semen treated P1, P2, and P3 didn’t different in all observation periods. The second and third period spermatozoa mass motility of P2 and P3 showed that the value of +++ was significantly different (P<0.05) compared to P1 (++); Individual motility of P1 treatment showed the lowest value 60,56±2,94 % (P<0.05) compared to P2 and P3 in the second and third periods (75,50±2,29 and 72,50±2,34%). The second and third periods of P2 and P3 spermatozoa concentrations showed an average of >1,350 million/cc significantly different (P<0.05) than P1 (876±152 million/cc), while the total motile spermatozoa showed the highest value of 5,647±829 million/cc in the second period P3. The spermatozoa viability of P2 and P3 showed values >88-89% significantly different (P<0.05) than P1 (<84%) in the second and third periods, while the best spermatozoa abnormalities were P2 at 4.30% in the second period and P3 at 5.33% in the third period. It was concluded that the giving of MO leaf powder at a dose of 0,1 kg/head/day could improve the quantity and quality of semen (viability and total motile spermatozoa) of Ongole Crossbreed bulls. Keywords: bulls, Moringa oleifera, quantity and quality of semen

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